﻿<110> University of Essex Enterprises Limited

<120> Modified Globin Proteins

<130> P031087WO

<150> GB 1704006.4
<151> 2017-03-13

<160> 18

<170> PatentIn version 3.5

<210> 1
<211> 146
<212> PRT
<213> Homo sapiens

<400> 1
Val His Leu Thr Pro Glu Glu Lys Ser Ala Val Thr Ala Leu Trp Gly 
1               5                   10                  15      
Lys Val Asn Val Asp Glu Val Gly Gly Glu Ala Leu Gly Arg Leu Leu 
            20                  25                  30          
Val Val Tyr Pro Trp Thr Gln Arg Phe Phe Glu Ser Phe Gly Asp Leu 
        35                  40                  45              
Ser Thr Pro Asp Ala Val Met Gly Asn Pro Lys Val Lys Ala His Gly 
    50                  55                  60                  
Lys Lys Val Leu Gly Ala Phe Ser Asp Gly Leu Ala His Leu Asp Asn 
65                  70                  75                  80  
Leu Lys Gly Thr Phe Ala Thr Leu Ser Glu Leu His Cys Asp Lys Leu 
                85                  90                  95      
His Val Asp Pro Glu Asn Phe Arg Leu Leu Gly Asn Val Leu Val Cys 
            100                 105                 110         
Val Leu Ala His His Phe Gly Lys Glu Phe Thr Pro Pro Val Gln Ala 
        115                 120                 125             
Ala Tyr Gln Lys Val Val Ala Gly Val Ala Asn Ala Leu Ala His Lys 
    130                 135                 140                 
Tyr His 
145     

<210> 2
<211> 141
<212> PRT
<213> Homo sapiens

<400> 2
Val Leu Ser Pro Ala Asp Lys Thr Asn Val Lys Ala Ala Trp Gly Lys 
1               5                   10                  15      
Val Gly Ala His Ala Gly Glu Tyr Gly Ala Glu Ala Leu Glu Arg Met 
            20                  25                  30          
Phe Leu Ser Phe Pro Thr Thr Lys Thr Tyr Phe Pro His Phe Asp Leu 
        35                  40                  45              
Ser His Gly Ser Ala Gln Val Lys Gly His Gly Lys Lys Val Ala Asp 
    50                  55                  60                  
Ala Leu Thr Asn Ala Val Ala His Val Asp Asp Met Pro Asn Ala Leu 
65                  70                  75                  80  
Ser Ala Leu Ser Asp Leu His Ala His Lys Leu Arg Val Asp Pro Val 
                85                  90                  95      
Asn Phe Lys Leu Leu Ser His Cys Leu Leu Val Thr Leu Ala Ala His 
            100                 105                 110         
Leu Pro Ala Glu Phe Thr Pro Ala Val His Ala Ser Leu Asp Lys Phe 
        115                 120                 125             
Leu Ala Ser Val Ser Thr Val Leu Thr Ser Lys Tyr Arg 
    130                 135                 140     

<210> 3
<211> 146
<212> PRT
<213> Homo sapiens

<400> 3
Gly His Phe Thr Glu Glu Asp Lys Ala Thr Ile Thr Ser Leu Trp Gly 
1               5                   10                  15      
Lys Val Asn Val Glu Asp Ala Gly Gly Glu Thr Leu Gly Arg Leu Leu 
            20                  25                  30          
Val Val Tyr Pro Trp Thr Gln Arg Phe Phe Asp Ser Phe Gly Asn Leu 
        35                  40                  45              
Ser Ser Ala Ser Ala Ile Met Gly Asn Pro Lys Val Lys Ala His Gly 
    50                  55                  60                  
Lys Lys Val Leu Thr Ser Leu Gly Asp Ala Ile Lys His Leu Asp Asp 
65                  70                  75                  80  
Leu Lys Gly Thr Phe Ala Gln Leu Ser Glu Leu His Cys Asp Lys Leu 
                85                  90                  95      
His Val Asp Pro Glu Asn Phe Lys Leu Leu Gly Asn Val Leu Val Thr 
            100                 105                 110         
Val Leu Ala Ile His Phe Gly Lys Glu Phe Thr Pro Glu Val Gln Ala 
        115                 120                 125             
Ser Trp Gln Lys Met Val Thr Ala Val Ala Ser Ala Leu Ser Ser Arg 
    130                 135                 140                 
Tyr His 
145     

<210> 4
<211> 146
<212> PRT
<213> Homo sapiens

<400> 4
Gly His Phe Thr Glu Glu Asp Lys Ala Thr Ile Thr Ser Leu Trp Gly 
1               5                   10                  15      
Lys Val Asn Val Glu Asp Ala Gly Gly Glu Thr Leu Gly Arg Leu Leu 
            20                  25                  30          
Val Val Tyr Pro Trp Thr Gln Arg Phe Phe Asp Ser Phe Gly Asn Leu 
        35                  40                  45              
Ser Ser Ala Ser Ala Ile Met Gly Asn Pro Lys Val Lys Ala His Gly 
    50                  55                  60                  
Lys Lys Val Leu Thr Ser Leu Gly Asp Ala Ile Lys His Leu Asp Asp 
65                  70                  75                  80  
Leu Lys Gly Thr Phe Ala Gln Leu Ser Glu Leu His Cys Asp Lys Leu 
                85                  90                  95      
His Val Asp Pro Glu Asn Phe Lys Leu Leu Gly Asn Val Leu Val Thr 
            100                 105                 110         
Val Leu Ala Ile His Phe Gly Lys Glu Phe Thr Pro Glu Val Gln Ala 
        115                 120                 125             
Ser Trp Gln Lys Met Val Thr Gly Val Ala Ser Ala Leu Ser Ser Arg 
    130                 135                 140                 
Tyr His 
145     

<210> 5
<211> 146
<212> PRT
<213> Artificial Sequence

<220> 
<223> βV1M (reference protein)

<400> 5
Met His Leu Thr Pro Glu Glu Lys Ser Ala Val Thr Ala Leu Trp Gly 
1               5                   10                  15      
Lys Val Asn Val Asp Glu Val Gly Gly Glu Ala Leu Gly Arg Leu Leu 
            20                  25                  30          
Val Val Tyr Pro Trp Thr Gln Arg Phe Phe Glu Ser Phe Gly Asp Leu 
        35                  40                  45              
Ser Thr Pro Asp Ala Val Met Gly Asn Pro Lys Val Lys Ala His Gly 
    50                  55                  60                  
Lys Lys Val Leu Gly Ala Phe Ser Asp Gly Leu Ala His Leu Asp Asn 
65                  70                  75                  80  
Leu Lys Gly Thr Phe Ala Thr Leu Ser Glu Leu His Cys Asp Lys Leu 
                85                  90                  95      
His Val Asp Pro Glu Asn Phe Arg Leu Leu Gly Asn Val Leu Val Cys 
            100                 105                 110         
Val Leu Ala His His Phe Gly Lys Glu Phe Thr Pro Pro Val Gln Ala 
        115                 120                 125             
Ala Tyr Gln Lys Val Val Ala Gly Val Ala Asn Ala Leu Ala His Lys 
    130                 135                 140                 
Tyr His 
145     

<210> 6
<211> 141
<212> PRT
<213> Artificial Sequence


<220> 
<223> αV1M mutant (reference protein)

<400> 6
Met Leu Ser Pro Ala Asp Lys Thr Asn Val Lys Ala Ala Trp Gly Lys 
1               5                   10                  15      
Val Gly Ala His Ala Gly Glu Tyr Gly Ala Glu Ala Leu Glu Arg Met 
            20                  25                  30          
Phe Leu Ser Phe Pro Thr Thr Lys Thr Tyr Phe Pro His Phe Asp Leu 
        35                  40                  45              
Ser His Gly Ser Ala Gln Val Lys Gly His Gly Lys Lys Val Ala Asp 
    50                  55                  60                  
Ala Leu Thr Asn Ala Val Ala His Val Asp Asp Met Pro Asn Ala Leu 
65                  70                  75                  80  
Ser Ala Leu Ser Asp Leu His Ala His Lys Leu Arg Val Asp Pro Val 
                85                  90                  95      
Asn Phe Lys Leu Leu Ser His Cys Leu Leu Val Thr Leu Ala Ala His 
            100                 105                 110         
Leu Pro Ala Glu Phe Thr Pro Ala Val His Ala Ser Leu Asp Lys Phe 
        115                 120                 125             
Leu Ala Ser Val Ser Thr Val Leu Thr Ser Lys Tyr Arg 
    130                 135                 140     

<210> 7
<211> 146
<212> PRT
<213> Artificial Sequence


<220> 
<223> γ1G1M (reference protein)

<400> 7
Met His Phe Thr Glu Glu Asp Lys Ala Thr Ile Thr Ser Leu Trp Gly 
1               5                   10                  15      
Lys Val Asn Val Glu Asp Ala Gly Gly Glu Thr Leu Gly Arg Leu Leu 
            20                  25                  30          
Val Val Tyr Pro Trp Thr Gln Arg Phe Phe Asp Ser Phe Gly Asn Leu 
        35                  40                  45              
Ser Ser Ala Ser Ala Ile Met Gly Asn Pro Lys Val Lys Ala His Gly 
    50                  55                  60                  
Lys Lys Val Leu Thr Ser Leu Gly Asp Ala Ile Lys His Leu Asp Asp 
65                  70                  75                  80  
Leu Lys Gly Thr Phe Ala Gln Leu Ser Glu Leu His Cys Asp Lys Leu 
                85                  90                  95      
His Val Asp Pro Glu Asn Phe Lys Leu Leu Gly Asn Val Leu Val Thr 
            100                 105                 110         
Val Leu Ala Ile His Phe Gly Lys Glu Phe Thr Pro Glu Val Gln Ala 
        115                 120                 125             
Ser Trp Gln Lys Met Val Thr Ala Val Ala Ser Ala Leu Ser Ser Arg 
    130                 135                 140                 
Tyr His 
145     

<210> 8
<211> 146
<212> PRT
<213> Artificial Sequence


<220> 
<223> γ2G1M (reference protein)

<400> 8
Met His Phe Thr Glu Glu Asp Lys Ala Thr Ile Thr Ser Leu Trp Gly 
1               5                   10                  15      
Lys Val Asn Val Glu Asp Ala Gly Gly Glu Thr Leu Gly Arg Leu Leu 
            20                  25                  30          
Val Val Tyr Pro Trp Thr Gln Arg Phe Phe Asp Ser Phe Gly Asn Leu 
        35                  40                  45              
Ser Ser Ala Ser Ala Ile Met Gly Asn Pro Lys Val Lys Ala His Gly 
    50                  55                  60                  
Lys Lys Val Leu Thr Ser Leu Gly Asp Ala Ile Lys His Leu Asp Asp 
65                  70                  75                  80  
Leu Lys Gly Thr Phe Ala Gln Leu Ser Glu Leu His Cys Asp Lys Leu 
                85                  90                  95      
His Val Asp Pro Glu Asn Phe Lys Leu Leu Gly Asn Val Leu Val Thr 
            100                 105                 110         
Val Leu Ala Ile His Phe Gly Lys Glu Phe Thr Pro Glu Val Gln Ala 
        115                 120                 125             
Ser Trp Gln Lys Met Val Thr Gly Val Ala Ser Ala Leu Ser Ser Arg 
    130                 135                 140                 
Tyr His 
145     

<210> 9
<211> 146
<212> PRT
<213> Artificial Sequence


<220> 
<223> βT84Y modified haemoglobin subunit

<400> 9
Val His Leu Thr Pro Glu Glu Lys Ser Ala Val Thr Ala Leu Trp Gly 
1               5                   10                  15      
Lys Val Asn Val Asp Glu Val Gly Gly Glu Ala Leu Gly Arg Leu Leu 
            20                  25                  30          
Val Val Tyr Pro Trp Thr Gln Arg Phe Phe Glu Ser Phe Gly Asp Leu 
        35                  40                  45              
Ser Thr Pro Asp Ala Val Met Gly Asn Pro Lys Val Lys Ala His Gly 
    50                  55                  60                  
Lys Lys Val Leu Gly Ala Phe Ser Asp Gly Leu Ala His Leu Asp Asn 
65                  70                  75                  80  
Leu Lys Gly Tyr Phe Ala Thr Leu Ser Glu Leu His Cys Asp Lys Leu 
                85                  90                  95      
His Val Asp Pro Glu Asn Phe Arg Leu Leu Gly Asn Val Leu Val Cys 
            100                 105                 110         
Val Leu Ala His His Phe Gly Lys Glu Phe Thr Pro Pro Val Gln Ala 
        115                 120                 125             
Ala Tyr Gln Lys Val Val Ala Gly Val Ala Asn Ala Leu Ala His Lys 
    130                 135                 140                 
Tyr His 
145     

<210> 10
<211> 146
<212> PRT
<213> Artificial Sequence


<220> 
<223> βT84Y modified haemoglobin subunit (V1M mutant)

<400> 10
Met His Leu Thr Pro Glu Glu Lys Ser Ala Val Thr Ala Leu Trp Gly 
1               5                   10                  15      
Lys Val Asn Val Asp Glu Val Gly Gly Glu Ala Leu Gly Arg Leu Leu 
            20                  25                  30          
Val Val Tyr Pro Trp Thr Gln Arg Phe Phe Glu Ser Phe Gly Asp Leu 
        35                  40                  45              
Ser Thr Pro Asp Ala Val Met Gly Asn Pro Lys Val Lys Ala His Gly 
    50                  55                  60                  
Lys Lys Val Leu Gly Ala Phe Ser Asp Gly Leu Ala His Leu Asp Asn 
65                  70                  75                  80  
Leu Lys Gly Tyr Phe Ala Thr Leu Ser Glu Leu His Cys Asp Lys Leu 
                85                  90                  95      
His Val Asp Pro Glu Asn Phe Arg Leu Leu Gly Asn Val Leu Val Cys 
            100                 105                 110         
Val Leu Ala His His Phe Gly Lys Glu Phe Thr Pro Pro Val Gln Ala 
        115                 120                 125             
Ala Tyr Gln Lys Val Val Ala Gly Val Ala Asn Ala Leu Ala His Lys 
    130                 135                 140                 
Tyr His 
145     

<210> 11
<211> 141
<212> PRT
<213> Artificial Sequence


<220> 
<223> αL91Y modified haemoglobin subunit (V1M mutant)

<400> 11
Met Leu Ser Pro Ala Asp Lys Thr Asn Val Lys Ala Ala Trp Gly Lys 
1               5                   10                  15      
Val Gly Ala His Ala Gly Glu Tyr Gly Ala Glu Ala Leu Glu Arg Met 
            20                  25                  30          
Phe Leu Ser Phe Pro Thr Thr Lys Thr Tyr Phe Pro His Phe Asp Leu 
        35                  40                  45              
Ser His Gly Ser Ala Gln Val Lys Gly His Gly Lys Lys Val Ala Asp 
    50                  55                  60                  
Ala Leu Thr Asn Ala Val Ala His Val Asp Asp Met Pro Asn Ala Leu 
65                  70                  75                  80  
Ser Ala Leu Ser Asp Leu His Ala His Lys Tyr Arg Val Asp Pro Val 
                85                  90                  95      
Asn Phe Lys Leu Leu Ser His Cys Leu Leu Val Thr Leu Ala Ala His 
            100                 105                 110         
Leu Pro Ala Glu Phe Thr Pro Ala Val His Ala Ser Leu Asp Lys Phe 
        115                 120                 125             
Leu Ala Ser Val Ser Thr Val Leu Thr Ser Lys Tyr Arg 
    130                 135                 140     

<210> 12
<211> 141
<212> PRT
<213> Artificial Sequence


<220> 
<223> αL91Y modified haemoglobin subunit

<400> 12
Val Leu Ser Pro Ala Asp Lys Thr Asn Val Lys Ala Ala Trp Gly Lys 
1               5                   10                  15      
Val Gly Ala His Ala Gly Glu Tyr Gly Ala Glu Ala Leu Glu Arg Met 
            20                  25                  30          
Phe Leu Ser Phe Pro Thr Thr Lys Thr Tyr Phe Pro His Phe Asp Leu 
        35                  40                  45              
Ser His Gly Ser Ala Gln Val Lys Gly His Gly Lys Lys Val Ala Asp 
    50                  55                  60                  
Ala Leu Thr Asn Ala Val Ala His Val Asp Asp Met Pro Asn Ala Leu 
65                  70                  75                  80  
Ser Ala Leu Ser Asp Leu His Ala His Lys Tyr Arg Val Asp Pro Val 
                85                  90                  95      
Asn Phe Lys Leu Leu Ser His Cys Leu Leu Val Thr Leu Ala Ala His 
            100                 105                 110         
Leu Pro Ala Glu Phe Thr Pro Ala Val His Ala Ser Leu Asp Lys Phe 
        115                 120                 125             
Leu Ala Ser Val Ser Thr Val Leu Thr Ser Lys Tyr Arg 
    130                 135                 140     

<210> 13
<211> 146
<212> PRT
<213> Artificial Sequence


<220> 
<223> γ2L96Y modified haemoglobin subunit (G1M Mutant)

<400> 13
Met His Phe Thr Glu Glu Asp Lys Ala Thr Ile Thr Ser Leu Trp Gly 
1               5                   10                  15      
Lys Val Asn Val Glu Asp Ala Gly Gly Glu Thr Leu Gly Arg Leu Leu 
            20                  25                  30          
Val Val Tyr Pro Trp Thr Gln Arg Phe Phe Asp Ser Phe Gly Asn Leu 
        35                  40                  45              
Ser Ser Ala Ser Ala Ile Met Gly Asn Pro Lys Val Lys Ala His Gly 
    50                  55                  60                  
Lys Lys Val Leu Thr Ser Leu Gly Asp Ala Ile Lys His Leu Asp Asp 
65                  70                  75                  80  
Leu Lys Gly Thr Phe Ala Gln Leu Ser Glu Leu His Cys Asp Lys Tyr 
                85                  90                  95      
His Val Asp Pro Glu Asn Phe Lys Leu Leu Gly Asn Val Leu Val Thr 
            100                 105                 110         
Val Leu Ala Ile His Phe Gly Lys Glu Phe Thr Pro Glu Val Gln Ala 
        115                 120                 125             
Ser Trp Gln Lys Met Val Thr Gly Val Ala Ser Ala Leu Ser Ser Arg 
    130                 135                 140                 
Tyr His 
145     

<210> 14
<211> 141
<212> PRT
<213> Artificial Sequence


<220> 
<223> αL29F modified haemoglobin subunit (V1M mutant)

<400> 14
Met Leu Ser Pro Ala Asp Lys Thr Asn Val Lys Ala Ala Trp Gly Lys 
1               5                   10                  15      
Val Gly Ala His Ala Gly Glu Tyr Gly Ala Glu Ala Phe Glu Arg Met 
            20                  25                  30          
Phe Leu Ser Phe Pro Thr Thr Lys Thr Tyr Phe Pro His Phe Asp Leu 
        35                  40                  45              
Ser His Gly Ser Ala Gln Val Lys Gly His Gly Lys Lys Val Ala Asp 
    50                  55                  60                  
Ala Leu Thr Asn Ala Val Ala His Val Asp Asp Met Pro Asn Ala Leu 
65                  70                  75                  80  
Ser Ala Leu Ser Asp Leu His Ala His Lys Leu Arg Val Asp Pro Val 
                85                  90                  95      
Asn Phe Lys Leu Leu Ser His Cys Leu Leu Val Thr Leu Ala Ala His 
            100                 105                 110         
Leu Pro Ala Glu Phe Thr Pro Ala Val His Ala Ser Leu Asp Lys Phe 
        115                 120                 125             
Leu Ala Ser Val Ser Thr Val Leu Thr Ser Lys Tyr Arg 
    130                 135                 140     

<210> 15
<211> 146
<212> PRT
<213> Artificial Sequence


<220> 
<223> γ2V67F modified haemoglobin subunit (G1M Mutant)

<400> 15
Met His Phe Thr Glu Glu Asp Lys Ala Thr Ile Thr Ser Leu Trp Gly 
1               5                   10                  15      
Lys Val Asn Val Glu Asp Ala Gly Gly Glu Thr Leu Gly Arg Leu Leu 
            20                  25                  30          
Val Val Tyr Pro Trp Thr Gln Arg Phe Phe Asp Ser Phe Gly Asn Leu 
        35                  40                  45              
Ser Ser Ala Ser Ala Ile Met Gly Asn Pro Lys Val Lys Ala His Gly 
    50                  55                  60                  
Lys Lys Phe Leu Thr Ser Leu Gly Asp Ala Ile Lys His Leu Asp Asp 
65                  70                  75                  80  
Leu Lys Gly Thr Phe Ala Gln Leu Ser Glu Leu His Cys Asp Lys Leu 
                85                  90                  95      
His Val Asp Pro Glu Asn Phe Lys Leu Leu Gly Asn Val Leu Val Thr 
            100                 105                 110         
Val Leu Ala Ile His Phe Gly Lys Glu Phe Thr Pro Glu Val Gln Ala 
        115                 120                 125             
Ser Trp Gln Lys Met Val Thr Gly Val Ala Ser Ala Leu Ser Ser Arg 
    130                 135                 140                 
Tyr His 
145     

<210> 16
<211> 141
<212> PRT
<213> Artificial Sequence


<220> 
<223> αL29F and αL91Y modified haemoglobin subunit (V1M mutant)

<400> 16
Met Leu Ser Pro Ala Asp Lys Thr Asn Val Lys Ala Ala Trp Gly Lys 
1               5                   10                  15      
Val Gly Ala His Ala Gly Glu Tyr Gly Ala Glu Ala Phe Glu Arg Met 
            20                  25                  30          
Phe Leu Ser Phe Pro Thr Thr Lys Thr Tyr Phe Pro His Phe Asp Leu 
        35                  40                  45              
Ser His Gly Ser Ala Gln Val Lys Gly His Gly Lys Lys Val Ala Asp 
    50                  55                  60                  
Ala Leu Thr Asn Ala Val Ala His Val Asp Asp Met Pro Asn Ala Leu 
65                  70                  75                  80  
Ser Ala Leu Ser Asp Leu His Ala His Lys Tyr Arg Val Asp Pro Val 
                85                  90                  95      
Asn Phe Lys Leu Leu Ser His Cys Leu Leu Val Thr Leu Ala Ala His 
            100                 105                 110         
Leu Pro Ala Glu Phe Thr Pro Ala Val His Ala Ser Leu Asp Lys Phe 
        115                 120                 125             
Leu Ala Ser Val Ser Thr Val Leu Thr Ser Lys Tyr Arg 
    130                 135                 140     

<210> 17
<211> 146
<212> PRT
<213> Artificial Sequence


<220> 
<223> γ2V67F and γ2L96Y modified haemoglobin subunit (G1M Mutant)

<400> 17
Met His Phe Thr Glu Glu Asp Lys Ala Thr Ile Thr Ser Leu Trp Gly 
1               5                   10                  15      
Lys Val Asn Val Glu Asp Ala Gly Gly Glu Thr Leu Gly Arg Leu Leu 
            20                  25                  30          
Val Val Tyr Pro Trp Thr Gln Arg Phe Phe Asp Ser Phe Gly Asn Leu 
        35                  40                  45              
Ser Ser Ala Ser Ala Ile Met Gly Asn Pro Lys Val Lys Ala His Gly 
    50                  55                  60                  
Lys Lys Phe Leu Thr Ser Leu Gly Asp Ala Ile Lys His Leu Asp Asp 
65                  70                  75                  80  
Leu Lys Gly Thr Phe Ala Gln Leu Ser Glu Leu His Cys Asp Lys Tyr 
                85                  90                  95      
His Val Asp Pro Glu Asn Phe Lys Leu Leu Gly Asn Val Leu Val Thr 
            100                 105                 110         
Val Leu Ala Ile His Phe Gly Lys Glu Phe Thr Pro Glu Val Gln Ala 
        115                 120                 125             
Ser Trp Gln Lys Met Val Thr Gly Val Ala Ser Ala Leu Ser Ser Arg 
    130                 135                 140                 
Tyr His 
145     

<210> 18
<211> 153
<212> PRT
<213> Artificial Sequence


<220> 
<223> Human myoglobin

<400> 18
Gly Leu Ser Asp Gly Glu Trp Gln Leu Val Leu Asn Val Trp Gly Lys 
1               5                   10                  15      
Val Glu Ala Asp Ile Pro Gly His Gly Gln Glu Val Leu Ile Arg Leu 
            20                  25                  30          
Phe Lys Gly His Pro Glu Thr Leu Glu Lys Phe Asp Lys Phe Lys His 
        35                  40                  45              
Leu Lys Ser Glu Asp Glu Met Lys Ala Ser Glu Asp Leu Lys Lys His 
    50                  55                  60                  
Gly Ala Thr Val Leu Thr Ala Leu Gly Gly Ile Leu Lys Lys Lys Gly 
65                  70                  75                  80  
His His Glu Ala Glu Ile Lys Pro Leu Ala Gln Ser His Ala Thr Lys 
                85                  90                  95      
His Lys Ile Pro Val Lys Tyr Leu Glu Phe Ile Ser Glu Cys Ile Ile 
            100                 105                 110         
Gln Val Leu Gln Ser Lys His Pro Gly Asp Phe Gly Ala Asp Ala Gln 
        115                 120                 125             
Gly Ala Met Asn Lys Ala Leu Glu Leu Phe Arg Lys Asp Met Ala Ser 
    130                 135                 140                 
Asn Tyr Lys Glu Leu Gly Phe Gln Gly 
145                 150             

