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<110>  ϾƼ޹˾
 
<120>  NKG2DLǶϿԭ弰CAR-TϸԼǵӦ

<130>  2017-NKG2DL-CAR-T

<160>  21    

<170>  PatentIn version 3.3

<210>  1
<211>  216
<212>  PRT
<213>  

<400>  1

Met Gly Trp Ile Arg Gly Arg Arg Ser Arg His Ser Trp Glu Met Ser 
1               5                   10                  15      


Glu Phe His Asn Tyr Asn Leu Asp Leu Lys Lys Ser Asp Phe Ser Thr 
            20                  25                  30          


Arg Trp Gln Lys Gln Arg Cys Pro Val Val Lys Ser Lys Cys Arg Glu 
        35                  40                  45              


Asn Ala Ser Pro Phe Phe Phe Cys Cys Phe Ile Ala Val Ala Met Gly 
    50                  55                  60                  


Ile Arg Phe Ile Ile Met Val Thr Ile Trp Ser Ala Val Phe Leu Asn 
65                  70                  75                  80  


Ser Leu Phe Asn Gln Glu Val Gln Ile Pro Leu Thr Glu Ser Tyr Cys 
                85                  90                  95      


Gly Pro Cys Pro Lys Asn Trp Ile Cys Tyr Lys Asn Asn Cys Tyr Gln 
            100                 105                 110         


Phe Phe Asp Glu Ser Lys Asn Trp Tyr Glu Ser Gln Ala Ser Cys Met 
        115                 120                 125             


Ser Gln Asn Ala Ser Leu Leu Lys Val Tyr Ser Lys Glu Asp Gln Asp 
    130                 135                 140                 


Leu Leu Lys Leu Val Lys Ser Tyr His Trp Met Gly Leu Val His Ile 
145                 150                 155                 160 


Pro Thr Asn Gly Ser Trp Gln Trp Glu Asp Gly Ser Ile Leu Ser Pro 
                165                 170                 175     


Asn Leu Leu Thr Ile Ile Glu Met Gln Lys Gly Asp Cys Ala Leu Tyr 
            180                 185                 190         


Ala Ser Ser Phe Lys Gly Tyr Ile Glu Asn Cys Ser Thr Pro Asn Thr 
        195                 200                 205             


Tyr Ile Cys Met Gln Arg Thr Val 
    210                 215     


<210>  2
<211>  651
<212>  DNA
<213>  

<400>  2
atggggtgga ttcgtggtcg gaggtctcga cacagctggg agatgagtga atttcataat     60

tataacttgg atctgaagaa gagtgatttt tcaacacgat ggcaaaagca aagatgtcca    120

gtagtcaaaa gcaaatgtag agaaaatgca tctccatttt ttttctgctg cttcatcgct    180

gtagccatgg gaatccgttt cattattatg gtaacaatat ggagtgctgt attcctaaac    240

tcattattca accaagaagt tcaaattccc ttgaccgaaa gttactgtgg cccatgtcct    300

aaaaactgga tatgttacaa aaataactgc taccaatttt ttgatgagag taaaaactgg    360

tatgagagcc aggcttcttg tatgtctcaa aatgccagcc ttctgaaagt atacagcaaa    420

gaggaccagg atttacttaa actggtgaag tcatatcatt ggatgggact agtacacatt    480

ccaacaaatg gatcttggca gtgggaagat ggctccattc tctcacccaa cctactaaca    540

ataattgaaa tgcagaaggg agactgtgca ctctatgcct cgagctttaa aggctatata    600

gaaaactgtt caactccaaa tacgtacatc tgcatgcaaa ggactgtgta a             651


<210>  3
<211>  21
<212>  PRT
<213>  

<400>  3

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  4
<211>  119
<212>  PRT
<213>  

<400>  4

Pro Cys Pro Lys Asn Trp Ile Cys Tyr Lys Asn Asn Cys Tyr Gln Phe 
1               5                   10                  15      


Phe Asp Glu Ser Lys Asn Trp Tyr Glu Ser Gln Ala Ser Cys Met Ser 
            20                  25                  30          


Gln Asn Ala Ser Leu Leu Lys Val Tyr Ser Lys Glu Asp Gln Asp Leu 
        35                  40                  45              


Leu Lys Leu Val Lys Ser Tyr His Trp Met Gly Leu Val His Ile Pro 
    50                  55                  60                  


Thr Asn Gly Ser Trp Gln Trp Glu Asp Gly Ser Ile Leu Ser Pro Asn 
65                  70                  75                  80  


Leu Leu Thr Ile Ile Glu Met Gln Lys Gly Asp Cys Ala Leu Tyr Ala 
                85                  90                  95      


Ser Ser Phe Lys Gly Tyr Ile Glu Asn Cys Ser Thr Pro Asn Thr Tyr 
            100                 105                 110         


Ile Cys Met Gln Arg Thr Val 
        115                 


<210>  5
<211>  136
<212>  PRT
<213>  

<400>  5

Ser Leu Phe Asn Gln Glu Val Gln Ile Pro Leu Thr Glu Ser Tyr Cys 
1               5                   10                  15      


Gly Pro Cys Pro Lys Asn Trp Ile Cys Tyr Lys Asn Asn Cys Tyr Gln 
            20                  25                  30          


Phe Phe Asp Glu Ser Lys Asn Trp Tyr Glu Ser Gln Ala Ser Cys Met 
        35                  40                  45              


Ser Gln Asn Ala Ser Leu Leu Lys Val Tyr Ser Lys Glu Asp Gln Asp 
    50                  55                  60                  


Leu Leu Lys Leu Val Lys Ser Tyr His Trp Met Gly Leu Val His Ile 
65                  70                  75                  80  


Pro Thr Asn Gly Ser Trp Gln Trp Glu Asp Gly Ser Ile Leu Ser Pro 
                85                  90                  95      


Asn Leu Leu Thr Ile Ile Glu Met Gln Lys Gly Asp Cys Ala Leu Tyr 
            100                 105                 110         


Ala Ser Ser Phe Lys Gly Tyr Ile Glu Asn Cys Ser Thr Pro Asn Thr 
        115                 120                 125             


Tyr Ile Cys Met Gln Arg Thr Val 
    130                 135     


<210>  6
<211>  152
<212>  PRT
<213>  

<400>  6

Ile Arg Phe Ile Ile Met Val Thr Ile Trp Ser Ala Val Phe Leu Asn 
1               5                   10                  15      


Ser Leu Phe Asn Gln Glu Val Gln Ile Pro Leu Thr Glu Ser Tyr Cys 
            20                  25                  30          


Gly Pro Cys Pro Lys Asn Trp Ile Cys Tyr Lys Asn Asn Cys Tyr Gln 
        35                  40                  45              


Phe Phe Asp Glu Ser Lys Asn Trp Tyr Glu Ser Gln Ala Ser Cys Met 
    50                  55                  60                  


Ser Gln Asn Ala Ser Leu Leu Lys Val Tyr Ser Lys Glu Asp Gln Asp 
65                  70                  75                  80  


Leu Leu Lys Leu Val Lys Ser Tyr His Trp Met Gly Leu Val His Ile 
                85                  90                  95      


Pro Thr Asn Gly Ser Trp Gln Trp Glu Asp Gly Ser Ile Leu Ser Pro 
            100                 105                 110         


Asn Leu Leu Thr Ile Ile Glu Met Gln Lys Gly Asp Cys Ala Leu Tyr 
        115                 120                 125             


Ala Ser Ser Phe Lys Gly Tyr Ile Glu Asn Cys Ser Thr Pro Asn Thr 
    130                 135                 140                 


Tyr Ile Cys Met Gln Arg Thr Val 
145                 150         


<210>  7
<211>  170
<212>  PRT
<213>  

<400>  7

Arg Glu Asn Ala Ser Pro Phe Phe Phe Cys Cys Phe Ile Ala Val Ala 
1               5                   10                  15      


Met Gly Ile Arg Phe Ile Ile Met Val Thr Ile Trp Ser Ala Val Phe 
            20                  25                  30          


Leu Asn Ser Leu Phe Asn Gln Glu Val Gln Ile Pro Leu Thr Glu Ser 
        35                  40                  45              


Tyr Cys Gly Pro Cys Pro Lys Asn Trp Ile Cys Tyr Lys Asn Asn Cys 
    50                  55                  60                  


Tyr Gln Phe Phe Asp Glu Ser Lys Asn Trp Tyr Glu Ser Gln Ala Ser 
65                  70                  75                  80  


Cys Met Ser Gln Asn Ala Ser Leu Leu Lys Val Tyr Ser Lys Glu Asp 
                85                  90                  95      


Gln Asp Leu Leu Lys Leu Val Lys Ser Tyr His Trp Met Gly Leu Val 
            100                 105                 110         


His Ile Pro Thr Asn Gly Ser Trp Gln Trp Glu Asp Gly Ser Ile Leu 
        115                 120                 125             


Ser Pro Asn Leu Leu Thr Ile Ile Glu Met Gln Lys Gly Asp Cys Ala 
    130                 135                 140                 


Leu Tyr Ala Ser Ser Phe Lys Gly Tyr Ile Glu Asn Cys Ser Thr Pro 
145                 150                 155                 160 


Asn Thr Tyr Ile Cys Met Gln Arg Thr Val 
                165                 170 


<210>  8
<211>  45
<212>  PRT
<213>  

<400>  8

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  9
<211>  24
<212>  PRT
<213>  

<400>  9

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      

Ser Leu Val Ile Thr Leu Tyr Cys 
            20                  


<210>  10
<211>  42
<212>  PRT
<213>  

<400>  10

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  11
<211>  112
<212>  PRT
<213>  

<400>  11

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  12
<211>  63
<212>  DNA
<213>  

<400>  12
atggccctgc ccgtcaccgc tctgctgctg ccccttgctc tgcttcttca tgcagcaagg     60

ccg                                                                   63


<210>  13
<211>  357
<212>  DNA
<213>  

<400>  13
ccctgcccaa agaactggat atgttacaaa aataattgct accagttttt cgacgagtcc     60

aagaattggt atgaatcaca agccagctgc atgtcccaaa atgcgtcatt gttgaaggta    120

tattctaagg aggaccaaga tttgttgaag ttggttaaat cctatcattg gatggggttg    180

gtccatatac ctacaaatgg ttcatggcag tgggaagatg gatctatact gagcccaaat    240

cttctgacaa taattgaaat gcaaaaaggc gattgtgccc tttacgctag tagcttcaaa    300

ggttatattg agaactgtag cacaccgaac acttatatct gtatgcagag aacggtt       357


<210>  14
<211>  408
<212>  DNA
<213>  

<400>  14
tctctgttca accaagaggt gcagatacca cttaccgaat catattgtgg cccctgccca     60

aagaactgga tatgttacaa aaataattgc taccagtttt tcgacgagtc caagaattgg    120

tatgaatcac aagccagctg catgtcccaa aatgcgtcat tgttgaaggt atattctaag    180

gaggaccaag atttgttgaa gttggttaaa tcctatcatt ggatggggtt ggtccatata    240

cctacaaatg gttcatggca gtgggaagat ggatctatac tgagcccaaa tcttctgaca    300

ataattgaaa tgcaaaaagg cgattgtgcc ctttacgcta gtagcttcaa aggttatatt    360

gagaactgta gcacaccgaa cacttatatc tgtatgcaga gaacggtt                 408


<210>  15
<211>  456
<212>  DNA
<213>  

<400>  15
attaggttta ttattatggt cacaatatgg tctgccgtat ttctcaactc tctgttcaac     60

caagaggtgc agataccact taccgaatca tattgtggcc cctgcccaaa gaactggata    120

tgttacaaaa ataattgcta ccagtttttc gacgagtcca agaattggta tgaatcacaa    180

gccagctgca tgtcccaaaa tgcgtcattg ttgaaggtat attctaagga ggaccaagat    240

ttgttgaagt tggttaaatc ctatcattgg atggggttgg tccatatacc tacaaatggt    300

tcatggcagt gggaagatgg atctatactg agcccaaatc ttctgacaat aattgaaatg    360

caaaaaggcg attgtgccct ttacgctagt agcttcaaag gttatattga gaactgtagc    420

acaccgaaca cttatatctg tatgcagaga acggtt                              456


<210>  16
<211>  510
<212>  DNA
<213>  

<400>  16
agagaaaatg ctagtccctt tttcttctgt tgctttattg cggtcgcaat ggggattagg     60

tttattatta tggtcacaat atggtctgcc gtatttctca actctctgtt caaccaagag    120

gtgcagatac cacttaccga atcatattgt ggcccctgcc caaagaactg gatatgttac    180

aaaaataatt gctaccagtt tttcgacgag tccaagaatt ggtatgaatc acaagccagc    240

tgcatgtccc aaaatgcgtc attgttgaag gtatattcta aggaggacca agatttgttg    300

aagttggtta aatcctatca ttggatgggg ttggtccata tacctacaaa tggttcatgg    360

cagtgggaag atggatctat actgagccca aatcttctga caataattga aatgcaaaaa    420

ggcgattgtg ccctttacgc tagtagcttc aaaggttata ttgagaactg tagcacaccg    480

aacacttata tctgtatgca gagaacggtt                                     510


<210>  17
<211>  135
<212>  DNA
<213>  

<400>  17
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg     60

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg    120

gacttcgcct gtgat                                                     135


<210>  18
<211>  72
<212>  DNA
<213>  

<400>  18
atctacatct gggcgccctt ggccgggact tgtggggtcc ttctcctgtc actggttatc     60

accctttact gc                                                         72


<210>  19
<211>  126
<212>  DNA
<213>  

<400>  19
aaacggggca gaaagaaact cctgtatata ttcaaacaac catttatgag accagtacaa     60

actactcaag aggaagatgg ctgtagctgc cgatttccag aagaagaaga aggaggatgt    120

gaactg                                                               126


<210>  20
<211>  336
<212>  DNA
<213>  

<400>  20
agagtgaagt tcagcaggag cgcagacgcc cccgcgtaca agcagggcca gaaccagctc     60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc    120

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat    180

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc    240

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc    300

tacgacgccc ttcacatgca ggccctgccc cctcgc                              336


<210>  21
<211>  1259
<212>  DNA
<213>  

<400>  21
tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg     60

tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca    120

cagtccccga gaagttgggg ggaggggtcg gcaattgatc cggtgcctag agaaggtggc    180

gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg    240

gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg    300

ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg    360

gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc    420

ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc    480

gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc    540

ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg    600

ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg    660

gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt    720

cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg    780

gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa    840

ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg    900

cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac    960

aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg   1020

cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg   1080

gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc   1140

cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt   1200

tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtga    1259




