                         SEQUENCE LISTING

<110>  The Trustees of the University of Pennsylvania
       Song, Wenchao
       Sato, Sakaya
       Miwa, Takashi
       Gullipalli, Damodar
 
<120>  ANTI-C5 ANTIBODIES AND USES THEREOF

<130>  046483-6156-00-WO.607244

<150>  US 62/467,498
<151>  2017-03-06

<160>  104   

<170>  PatentIn version 3.5

<210>  1
<211>  432
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of VH of mAb 2G1

<400>  1
atgggatgga gctggatctt tctcctcttc ctgtcaggaa ctgcaggtgt cctctctgag       60

gtccagctgc aacagtctgg accagagctg gtgaagcctg gggcttcagt gaagatatcc      120

tgcaaggctt ctggatacac aatcacagac tacaatttgg actgggtgaa gcagagccat      180

ggaaagagcc ttgagtggat tggagatatt agtcctaact atggttatac tatctacaac      240

cagaaattca aggacaaggc cacattgact gtagacaggt cctccagcac agcctacatg      300

gagctccgca gcctgacatc tgaggacact gcagtttatt actgtgcaag aagggacatt      360

cgttactccg gtaattccta caaatggtac ttcgatgtct ggggcacagg gaccacggtc      420

accgtctcct ca                                                          432


<210>  2
<211>  144
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VH of mAb 2G1

<400>  2

Met Gly Trp Ser Trp Ile Phe Leu Leu Phe Leu Ser Gly Thr Ala Gly 
1               5                   10                  15      


Val Leu Ser Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Ile 
        35                  40                  45              


Thr Asp Tyr Asn Leu Asp Trp Val Lys Gln Ser His Gly Lys Ser Leu 
    50                  55                  60                  


Glu Trp Ile Gly Asp Ile Ser Pro Asn Tyr Gly Tyr Thr Ile Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Val Asp Arg Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Arg Asp Ile Arg Tyr Ser Gly Asn Ser Tyr Lys 
        115                 120                 125             


Trp Tyr Phe Asp Val Trp Gly Thr Gly Thr Thr Val Thr Val Ser Ser 
    130                 135                 140                 


<210>  3
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR1 of VH of mAb 2G1

<400>  3

Gly Tyr Thr Ile Thr Asp Tyr Asn Leu Asp 
1               5                   10  


<210>  4
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR2 of VH of mAb 2G1

<400>  4

Asp Ile Ser Pro Asn Tyr Gly Tyr Thr Ile Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Asp 
    


<210>  5
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR3 of VH of mAb 2G1

<400>  5

Arg Asp Ile Arg Tyr Ser Gly Asn Ser Tyr Lys Trp Tyr Phe Asp Val 
1               5                   10                  15      


<210>  6
<211>  381
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of VL of mAb 2G1

<400>  6
atgaggttcc aggttcaggt tctggggctc cttctgctct ggatatcagg tgcccagtgt       60

gatgtccaga taacccagtc tccatcttat cttgctgcat ctcctggaga aaccattact      120

attaattgca ggacaagtaa gagcataagc aaatatttag cctggtatca agagaaacct      180

gggaaaacta ataagcttct tatctactct ggatccacct tgcaatctgg aattccatca      240

aggttcaggg gcagtggatc tggtacagat ttcactctca ccatcagtag cctggagcct      300

gaagattttg caatgtatta ctgtcaacaa cataatgaat acccgtacac gttcggaggg      360

gggaccaagc tggaaataaa a                                                381


<210>  7
<211>  127
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VL of mAb 2G1

<400>  7

Met Arg Phe Gln Val Gln Val Leu Gly Leu Leu Leu Leu Trp Ile Ser 
1               5                   10                  15      


Gly Ala Gln Cys Asp Val Gln Ile Thr Gln Ser Pro Ser Tyr Leu Ala 
            20                  25                  30          


Ala Ser Pro Gly Glu Thr Ile Thr Ile Asn Cys Arg Thr Ser Lys Ser 
        35                  40                  45              


Ile Ser Lys Tyr Leu Ala Trp Tyr Gln Glu Lys Pro Gly Lys Thr Asn 
    50                  55                  60                  


Lys Leu Leu Ile Tyr Ser Gly Ser Thr Leu Gln Ser Gly Ile Pro Ser 
65                  70                  75                  80  


Arg Phe Arg Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
                85                  90                  95      


Ser Leu Glu Pro Glu Asp Phe Ala Met Tyr Tyr Cys Gln Gln His Asn 
            100                 105                 110         


Glu Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
        115                 120                 125         


<210>  8
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR1 of VL of mAb 2G1

<400>  8

Arg Thr Ser Lys Ser Ile Ser Lys Tyr Leu Ala 
1               5                   10      


<210>  9
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR2 of VL of mAb 2G1

<400>  9

Ser Gly Ser Thr Leu Gln Ser 
1               5           


<210>  10
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR3 of VL of mAb 2G1

<400>  10

Gln Gln His Asn Glu Tyr Pro Tyr Thr 
1               5                   


<210>  11
<211>  414
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of VH of mAb 8E1

<400>  11
atggctgtcc tggggctgct tctctgcctg gtgactttcc caagctgtgt cctgtcccag       60

gtgcagctga aggagtcagg acctggcctg gtggcgccct cacagagcct gtccatcaca      120

tgcaccgtct cagggttccc tttaaacaac tatggaatac actgggttcg ccagcctcca      180

ggaaagggtc tggagtggct ggcagtgata tggagtgatg gaagaacaac ctataattca      240

gctctcaact ccagactgag catcagcaag gacaactcca agagccaagt tttctttaaa      300

atgaacagtc tccaaactga tgacacagcc atgtactatt gtgccagaca tgatggtcgg      360

ggggactata gtatggacta ctggggtcaa ggaacctcag tcaccgtctc ctca            414


<210>  12
<211>  138
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VH of mAb 8E1

<400>  12

Met Ala Val Leu Gly Leu Leu Leu Cys Leu Val Thr Phe Pro Ser Cys 
1               5                   10                  15      


Val Leu Ser Gln Val Gln Leu Lys Glu Ser Gly Pro Gly Leu Val Ala 
            20                  25                  30          


Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Pro Leu 
        35                  40                  45              


Asn Asn Tyr Gly Ile His Trp Val Arg Gln Pro Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Leu Ala Val Ile Trp Ser Asp Gly Arg Thr Thr Tyr Asn Ser 
65                  70                  75                  80  


Ala Leu Asn Ser Arg Leu Ser Ile Ser Lys Asp Asn Ser Lys Ser Gln 
                85                  90                  95      


Val Phe Phe Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Met Tyr 
            100                 105                 110         


Tyr Cys Ala Arg His Asp Gly Arg Gly Asp Tyr Ser Met Asp Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
    130                 135             


<210>  13
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR1 of VH of mAb 8E1

<400>  13

Gly Phe Pro Leu Asn Asn Tyr Gly Ile His 
1               5                   10  


<210>  14
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR2 of VH of mAb 8E1

<400>  14

Val Ile Trp Ser Asp Gly Arg Thr Thr Tyr Asn Ser Ala Leu Asn Ser 
1               5                   10                  15      


<210>  15
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR3 of VH of mAb 8E1

<400>  15

His Asp Gly Arg Gly Asp Tyr Ser Met Asp Tyr 
1               5                   10      


<210>  16
<211>  390
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of VL of mAb 8E1

<400>  16
atggagacag acacactcct gttatgggta ctgctgctct gggcccttag ttccgagctc       60

gtgatgacac agtctcctgc ttccttagtt gtatctctgg ggcagagggc caccatctca      120

tgcagggcca gcaaaggtgt cagtacatct gtctacagtt atatgcactg gtaccaacag      180

aaaccaggac agccacccaa actcctcatc tatcttgcat ccaacctaga atctggggtc      240

cctgccaggt tcagtggcag tgggtctggg acagacttct ccctcaacat ccatcctgtg      300

gaggaggagg atgctgcaac ctattactgt cagcaaagtg gggagcttcc gctcacgttc      360

ggtgctggga ccaagctgga gctgaaacgg                                       390


<210>  17
<211>  130
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VL of mAb 8E1

<400>  17

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Ala Leu 
1               5                   10                  15      


Ser Ser Glu Leu Val Met Thr Gln Ser Pro Ala Ser Leu Val Val Ser 
            20                  25                  30          


Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Lys Gly Val Ser 
        35                  40                  45              


Thr Ser Val Tyr Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Gln 
    50                  55                  60                  


Pro Pro Lys Leu Leu Ile Tyr Leu Ala Ser Asn Leu Glu Ser Gly Val 
65                  70                  75                  80  


Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn 
                85                  90                  95      


Ile His Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln 
            100                 105                 110         


Ser Gly Glu Leu Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 
        115                 120                 125             


Lys Arg 
    130 


<210>  18
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR1 of VL of mAb 8E1

<400>  18

Arg Ala Ser Lys Gly Val Ser Thr Ser Val Tyr Ser Tyr Met His 
1               5                   10                  15  


<210>  19
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR2 of VL of mAb 8E1

<400>  19

Leu Ala Ser Asn Leu Glu Ser 
1               5           


<210>  20
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR3 of VL of mAb 8E1

<400>  20

Gln Gln Ser Gly Glu Leu Pro Leu Thr 
1               5                   


<210>  21
<211>  429
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of VH of mAb 4E7

<400>  21
atgtcctctc ctcagacact gaacacactg actctaacca tgggatggag ctggatcttt       60

ctctttctcc tgtcagaaac tgcaggagtc ctctctgagg tccagctgca acagggggct      120

tcagtgaaga tgtcctgtaa gacttctcga tactcattca ctgactacat catgcactgg      180

gtgaagctga gccatggaaa gagccttgag tggattggat atattaaccc taacaatggt      240

ggtactatct acaaccagaa gttcaaggac aaggccacat tgactgtaaa caagtcctcc      300

agcacagcct acatggaatt ccgcagcctg acatcggagg attctgcagt ctatttctgt      360

tcaagagggg gggtttatta ccgggggttt gcttactggg gccaagggac actggtcact      420

gtctctgca                                                              429


<210>  22
<211>  143
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VH of mAb 4E7

<400>  22

Met Ser Ser Pro Gln Thr Leu Asn Thr Leu Thr Leu Thr Met Gly Trp 
1               5                   10                  15      


Ser Trp Ile Phe Leu Phe Leu Leu Ser Glu Thr Ala Gly Val Leu Ser 
            20                  25                  30          


Glu Val Gln Leu Gln Gln Gly Ala Ser Val Lys Met Ser Cys Lys Thr 
        35                  40                  45              


Ser Arg Tyr Ser Phe Thr Asp Tyr Ile Met His Trp Val Lys Leu Ser 
    50                  55                  60                  


His Gly Lys Ser Leu Glu Trp Ile Gly Tyr Ile Asn Pro Asn Asn Gly 
65                  70                  75                  80  


Gly Thr Ile Tyr Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Val 
                85                  90                  95      


Asn Lys Ser Ser Ser Thr Ala Tyr Met Glu Phe Arg Ser Leu Thr Ser 
            100                 105                 110         


Glu Asp Ser Ala Val Tyr Phe Cys Ser Arg Gly Gly Val Tyr Tyr Arg 
        115                 120                 125             


Gly Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala 
    130                 135                 140             


<210>  23
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR1 of VH of mAb 4E7

<400>  23

Arg Tyr Ser Phe Thr Asp Tyr Ile Met 
1               5                   


<210>  24
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR2 of VH of mAb 4E7

<400>  24

Tyr Ile Asn Pro Asn Asn Gly Gly Thr Ile Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Asp 
    


<210>  25
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR3 of VH of mAb 4E7

<400>  25

Gly Gly Val Tyr Tyr Arg Gly Phe Ala Tyr 
1               5                   10  


<210>  26
<211>  411
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of VL of mAb 4E7

<400>  26
atggcctgga tttcacttat actctctctc ctggctctca gctcaggggc catttcccag       60

gctgttgtga ctcaggaatc tgcactcacc acatcacctg gtgaaacagt cacattcact      120

tgtcgctcaa gtactggggc tgttacaaac agtaactatg ccaactgggt ccaagaaaaa      180

ccagatcatt ttttcactgg tctaataggt gttaccaaca accgaggtcc aggtgttcct      240

gcccgattct caggctccct gattggagac aaggctgccc tcaccatcac aggggcacag      300

actgaggatg aggcaatata tttctgtgct ctatggtaca gcaaccactt gggtgttcgg      360

tggaggaacc aaactgactg tcctaggcca gcccaagtct tcgccatcag t               411


<210>  27
<211>  137
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VL of mAb 4E7

<400>  27

Met Ala Trp Ile Ser Leu Ile Leu Ser Leu Leu Ala Leu Ser Ser Gly 
1               5                   10                  15      


Ala Ile Ser Gln Ala Val Val Thr Gln Glu Ser Ala Leu Thr Thr Ser 
            20                  25                  30          


Pro Gly Glu Thr Val Thr Phe Thr Cys Arg Ser Ser Thr Gly Ala Val 
        35                  40                  45              


Thr Asn Ser Asn Tyr Ala Asn Trp Val Gln Glu Lys Pro Asp His Phe 
    50                  55                  60                  


Phe Thr Gly Leu Ile Gly Val Thr Asn Asn Arg Gly Pro Gly Val Pro 
65                  70                  75                  80  


Ala Arg Phe Ser Gly Ser Leu Ile Gly Asp Lys Ala Ala Leu Thr Ile 
                85                  90                  95      


Thr Gly Ala Gln Thr Glu Asp Glu Ala Ile Tyr Phe Cys Ala Leu Trp 
            100                 105                 110         


Tyr Ser Asn His Leu Gly Val Arg Trp Arg Asn Gln Thr Asp Cys Pro 
        115                 120                 125             


Arg Pro Ala Gln Val Phe Ala Ile Ser 
    130                 135         


<210>  28
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR1 of VL of mAb 4E7

<400>  28

Arg Ser Ser Thr Gly Ala Val Thr Asn Ser Asn Tyr Ala Asn 
1               5                   10                  


<210>  29
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR2 of VL of mAb 4E7

<400>  29

Val Thr Asn Asn Arg Gly Pro 
1               5           


<210>  30
<211>  423
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of VH of mAb 9G6

<400>  30
atggattggg tgtggacctt ggtattcctg atagcagctg cccaaagtgc ccaagcacag       60

atccagttgg tgcagtctgg acctgagctg aagaagcctg gagagacagt caagatctcc      120

tgcaaggctt ctgggtatac cttcacagaa tatccaatgc actgggtgaa gcaggctcca      180

ggaaagagtt tcaagtggat gggcatccta tacaccgaca ctggagagcc aacatatgct      240

gaagagttca ggggacggtt tgccttctct ttggagacct ctgccagcac tgcctatttg      300

cagatcaaca acctcaaaaa tgaggacacg gctacatatt tctgtgttca ctcaggctac      360

gtaggctact ggggccaagg caccactctc acagtctcat cagccaaaac aacaccccca      420

tca                                                                    423


<210>  31
<211>  134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VH of mAb 9G6

<400>  31

Met Asp Trp Val Trp Thr Leu Val Phe Leu Ile Ala Ala Ala Gln Ser 
1               5                   10                  15      


Ala Gln Ala Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys 
            20                  25                  30          


Pro Gly Glu Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Glu Tyr Pro Met His Trp Val Lys Gln Ala Pro Gly Lys Ser Phe 
    50                  55                  60                  


Lys Trp Met Gly Ile Leu Tyr Thr Asp Thr Gly Glu Pro Thr Tyr Ala 
65                  70                  75                  80  


Glu Glu Phe Arg Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser 
                85                  90                  95      


Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr 
            100                 105                 110         


Tyr Phe Cys Val His Ser Gly Tyr Val Gly Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Thr Leu Thr Val Ser Ser 
    130                 


<210>  32
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR1 of VH of mAb 9G6

<400>  32

Gly Tyr Thr Phe Thr Glu Tyr Pro Met His 
1               5                   10  


<210>  33
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR2 of VH of mAb 9G6

<400>  33

Ile Leu Tyr Thr Asp Thr Gly Glu Pro Thr Tyr Ala Glu Glu Phe Arg 
1               5                   10                  15      


Gly 
    


<210>  34
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR3 of VH of mAb 9G6

<400>  34

Ser Gly Tyr Val Gly Tyr 
1               5       


<210>  35
<211>  384
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of VL of mAb 9G6

<400>  35
atgagtgtgc ccactcaggt cctggggttg ctgctgctgt ggcttacagg tgccagatgt       60

gacatccaga tgactcagtc tccagcctcc ctatcagcat ctgtgggaga aactgtcacc      120

ttcacatgtc gggcaagtga gattatttac agttatttag tttggtatca gcagaaacag      180

ggaaaatctc ctcagctcct ggtctctaat gcaaaaacct tagcagaggg tgtgccatca      240

aggttcagtg gcagtggatc aggcacacag ttttctctga agatcaatag cctgcagcct      300

gaagattttg ggagttatta ctgtcaacat tattatggta atcctcccac gttcggaggg      360

gggaccaagc tggaaataaa acgg                                             384


<210>  36
<211>  128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VL of mAb 9G6

<400>  36

Met Ser Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr 
1               5                   10                  15      


Gly Ala Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ala Ser Leu Ser 
            20                  25                  30          


Ala Ser Val Gly Glu Thr Val Thr Phe Thr Cys Arg Ala Ser Glu Ile 
        35                  40                  45              


Ile Tyr Ser Tyr Leu Val Trp Tyr Gln Gln Lys Gln Gly Lys Ser Pro 
    50                  55                  60                  


Gln Leu Leu Val Ser Asn Ala Lys Thr Leu Ala Glu Gly Val Pro Ser 
65                  70                  75                  80  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Gln Phe Ser Leu Lys Ile Asn 
                85                  90                  95      


Ser Leu Gln Pro Glu Asp Phe Gly Ser Tyr Tyr Cys Gln His Tyr Tyr 
            100                 105                 110         


Gly Asn Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
        115                 120                 125             


<210>  37
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR1 of VL of mAb 9G6

<400>  37

Arg Ala Ser Glu Ile Ile Tyr Ser Tyr Leu Val 
1               5                   10      


<210>  38
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR2 of VL of mAb 9G6

<400>  38

Asn Ala Lys Thr Leu Ala Glu 
1               5           


<210>  39
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR3 of VL of mAb 9G6

<400>  39

Gln His Tyr Tyr Gly Asn Pro Pro Thr 
1               5                   


<210>  40
<211>  447
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of VH of mAb 11C5

<400>  40
atgagcactg aacacggacc cctcaccatg aacttcgggc tcagcttgat tttccttgtc       60

cttgttttaa aaggtgtcca gtgtgaagtg cagctagtgg agtctggggg aggcttagtg      120

aagcctggag ggtccctgaa actctcctgt gcagcctctg gattcacttt cactacctat      180

gccatgtctt gggttcgcca gactccggaa aagaggctgg agtgggtcgc aaccattagt      240

gctggtggta cttacaccta ctattcagac aatgtaaagg gccgattcac catctccaga      300

gacaatgcca agaacaacct gtacctgcaa atgagccatc tgaagtctga ggacacagcc      360

atgttttact gtgcaagaga tcccgattac tacggtagaa gcccgtttgc ttactggggc      420

caagggactc tggtcactgt ctctgca                                          447


<210>  41
<211>  149
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VH of mAb 11C5

<400>  41

Met Ser Thr Glu His Gly Pro Leu Thr Met Asn Phe Gly Leu Ser Leu 
1               5                   10                  15      


Ile Phe Leu Val Leu Val Leu Lys Gly Val Gln Cys Glu Val Gln Leu 
            20                  25                  30          


Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly Ser Leu Lys Leu 
        35                  40                  45              


Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Thr Tyr Ala Met Ser Trp 
    50                  55                  60                  


Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val Ala Thr Ile Ser 
65                  70                  75                  80  


Ala Gly Gly Thr Tyr Thr Tyr Tyr Ser Asp Asn Val Lys Gly Arg Phe 
                85                  90                  95      


Thr Ile Ser Arg Asp Asn Ala Lys Asn Asn Leu Tyr Leu Gln Met Ser 
            100                 105                 110         


His Leu Lys Ser Glu Asp Thr Ala Met Phe Tyr Cys Ala Arg Asp Pro 
        115                 120                 125             


Asp Tyr Tyr Gly Arg Ser Pro Phe Ala Tyr Trp Gly Gln Gly Thr Leu 
    130                 135                 140                 


Val Thr Val Ser Ala 
145                 


<210>  42
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR1 of VH of mAb 11C5

<400>  42

Gly Phe Thr Phe Thr Thr Tyr Ala Met Ser 
1               5                   10  


<210>  43
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR2 of VH of mAb 11C5

<400>  43

Thr Ile Ser Ala Gly Gly Thr Tyr Thr Tyr Tyr Ser Asp Asn Val Lys 
1               5                   10                  15      


Gly 
    


<210>  44
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR3 of VH of mAb 11C5

<400>  44

Asp Pro Asp Tyr Tyr Gly Arg Ser Pro Phe Ala Tyr 
1               5                   10          


<210>  45
<211>  384
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of VL of mAb 11C5

<400>  45
atggttttca cacctcagat acttggactt atgctttttt ggatttcagc ctccagaggt       60

gaaattgtgc tcactcagtc tccagccacc ctgtctgtga ctccaggaga gagcgtcagt      120

ctttcctgta gggccagcca aagtattagc aacaacctac agtggtatca acaaaaatca      180

catgagtctc caagacttct catcaaatat gcttcccagt ccatctctgg gatcccctcc      240

aggttcagtg gcagtggatc agggacagat ttcactctca gtatcaacag tgtggagact      300

gaagattttg gaatgtattt ctgtcaacag agtaacagct ggcctctcac gttcggtggt      360

gggaccaagg tggagctgaa acgg                                             384


<210>  46
<211>  128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequenceof VL of mAb 11C5

<400>  46

Met Val Phe Thr Pro Gln Ile Leu Gly Leu Met Leu Phe Trp Ile Ser 
1               5                   10                  15      


Ala Ser Arg Gly Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser 
            20                  25                  30          


Val Thr Pro Gly Glu Ser Val Ser Leu Ser Cys Arg Ala Ser Gln Ser 
        35                  40                  45              


Ile Ser Asn Asn Leu Gln Trp Tyr Gln Gln Lys Ser His Glu Ser Pro 
    50                  55                  60                  


Arg Leu Leu Ile Lys Tyr Ala Ser Gln Ser Ile Ser Gly Ile Pro Ser 
65                  70                  75                  80  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Ser Ile Asn 
                85                  90                  95      


Ser Val Glu Thr Glu Asp Phe Gly Met Tyr Phe Cys Gln Gln Ser Asn 
            100                 105                 110         


Ser Trp Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Leu Lys Arg 
        115                 120                 125             


<210>  47
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR1 of VL of mAb 11C5

<400>  47

Arg Ala Ser Gln Ser Ile Ser Asn Asn Leu Gln 
1               5                   10      


<210>  48
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR2 of VL of mAb 11C5

<400>  48

Tyr Ala Ser Gln Ser Ile Ser 
1               5           


<210>  49
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR3 of VL of mAb 11C5

<400>  49

Gln Gln Ser Asn Ser Trp Pro Leu Thr 
1               5                   


<210>  50
<211>  465
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of VH of mAb 11D9

<400>  50
atgtcctcac cacagacact gaacacactg actctaacca tgggatggag ctggatcttt       60

ctctttctcc tgtcaggaac tgcaggtgtc ctctctgagg tccagctgca acaatctgga      120

cctgaggtgg tgaagcctgg ggcttcagtg aagatatcct gtaaggcttc tggatacacg      180

ttcactgact tctacatgaa ctgggtgaaa cagagccatg gaaagagcct tgagtggatt      240

ggatatatta atcctaacaa tggtgatact agttacaacc agaagttcaa gggcaaggcc      300

acatcgactg tagacaggtc ctccaacaca gcctacatgg agctccgcag cctgacatct      360

gaggactctg cagtctatta ctgtgcaaga ctcatcttct atggtaactg gtactttgat      420

gtctggggca cagggaccac ggtcaccgtc tcctcagcca aaaca                      465


<210>  51
<211>  155
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VH of mAb 11D9

<400>  51

Met Ser Ser Pro Gln Thr Leu Asn Thr Leu Thr Leu Thr Met Gly Trp 
1               5                   10                  15      


Ser Trp Ile Phe Leu Phe Leu Leu Ser Gly Thr Ala Gly Val Leu Ser 
            20                  25                  30          


Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Val Val Lys Pro Gly Ala 
        35                  40                  45              


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Phe 
    50                  55                  60                  


Tyr Met Asn Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile 
65                  70                  75                  80  


Gly Tyr Ile Asn Pro Asn Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
                85                  90                  95      


Lys Gly Lys Ala Thr Ser Thr Val Asp Arg Ser Ser Asn Thr Ala Tyr 
            100                 105                 110         


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
        115                 120                 125             


Ala Arg Leu Ile Phe Tyr Gly Asn Trp Tyr Phe Asp Val Trp Gly Thr 
    130                 135                 140                 


Gly Thr Thr Val Thr Val Ser Ser Ala Lys Thr 
145                 150                 155 


<210>  52
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR1 of VH of mAb 11D9

<400>  52

Gly Tyr Thr Phe Thr Asp Phe Tyr Met Asn 
1               5                   10  


<210>  53
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR2 of VH of mAb 11D9

<400>  53

Tyr Ile Asn Pro Asn Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  54
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR3 of VH of mAb 11D9

<400>  54

Leu Ile Phe Tyr Gly Asn Trp Tyr Phe Asp Val 
1               5                   10      


<210>  55
<211>  393
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of VL of mAb 11D9

<400>  55
atggattttc agatgcagat tatcagcttg ctgctaatca gtgtcacagt catagtgtct       60

aatggagaaa ttgtgctcac ccagtctcca cccaccatgg ctgcatctcc cggggagaag      120

atcactatca cctgcagtgc cagctcaagt ataagttcca attacgtgca ttggtatcag      180

cagaagccag gattctcccc taaactcttg atttatagga catccaatct ggcttctgga      240

gtcccagttc gcttcagtgg cagtgggtct gggacctctt actctctcac aattggcacc      300

atggaggctg aagatgttgc cacttactac tgccagcagg gtactagtat accgtggacg      360

ttcggtggag gcaccaaggt ggaaattaat cgg                                   393


<210>  56
<211>  131
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VL of mAb 11D9

<400>  56

Met Asp Phe Gln Met Gln Ile Ile Ser Leu Leu Leu Ile Ser Val Thr 
1               5                   10                  15      


Val Ile Val Ser Asn Gly Glu Ile Val Leu Thr Gln Ser Pro Pro Thr 
            20                  25                  30          


Met Ala Ala Ser Pro Gly Glu Lys Ile Thr Ile Thr Cys Ser Ala Ser 
        35                  40                  45              


Ser Ser Ile Ser Ser Asn Tyr Val His Trp Tyr Gln Gln Lys Pro Gly 
    50                  55                  60                  


Phe Ser Pro Lys Leu Leu Ile Tyr Arg Thr Ser Asn Leu Ala Ser Gly 
65                  70                  75                  80  


Val Pro Val Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu 
                85                  90                  95      


Thr Ile Gly Thr Met Glu Ala Glu Asp Val Ala Thr Tyr Tyr Cys Gln 
            100                 105                 110         


Gln Gly Thr Ser Ile Pro Trp Thr Phe Gly Gly Gly Thr Lys Val Glu 
        115                 120                 125             


Ile Asn Arg 
    130     


<210>  57
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR1 of VL of mAb 11D9

<400>  57

Ser Ala Ser Ser Ser Ile Ser Ser Asn Tyr Val His 
1               5                   10          


<210>  58
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR2 of VL of mAb 11D9

<400>  58

Arg Thr Ser Asn Leu Ala Ser 
1               5           


<210>  59
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR3 of VL of mAb 11D9

<400>  59

Gln Gln Gly Thr Ser Ile Pro Trp Thr 
1               5                   


<210>  60
<211>  327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of human IgG4 Constant Heavy chain region 
       with S228P mutation

<400>  60

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Pro Gly Lys 
                325         


<210>  61
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of human Kappa Constant Light region

<400>  61

Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
1               5                   10                  15      


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
            20                  25                  30          


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
        35                  40                  45              


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
    50                  55                  60                  


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
65                  70                  75                  80  


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
                85                  90                  95      


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105     


<210>  62
<211>  432
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of Humanized 2G1 VH-11801

<400>  62
atggactgga cctgggtctt tctcttcctc ctgtcagtaa ctgcaggtgt ccactcccag       60

gttcagctgg tgcagtctgg agctgaggtg aagaagcctg gggcctcagt gaaggtctcc      120

tgcaaggctt ctggatacac aatcacagac tacaatttgg actgggtgcg acaggcccct      180

ggacaagggc ttgagtggat gggagatatt agtcctaact atggttatac tatctacaac      240

cagaaattca aggacagagt caccatgacc acagacacat ccacgagcac agcctacatg      300

gagctgagga gcctgagatc tgacgacacg gccgtgtatt actgtgcgag aagggacatt      360

cgttactccg gtaattccta caaatggtac ttcgatgtct ggggccaagg gacaatggtc      420

accgtctctt ca                                                          432


<210>  63
<211>  144
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of Humanized 2G1 VH-11801

<400>  63

Met Asp Trp Thr Trp Val Phe Leu Phe Leu Leu Ser Val Thr Ala Gly 
1               5                   10                  15      


Val His Ser Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Ile 
        35                  40                  45              


Thr Asp Tyr Asn Leu Asp Trp Val Arg Gln Ala Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Met Gly Asp Ile Ser Pro Asn Tyr Gly Tyr Thr Ile Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Lys Asp Arg Val Thr Met Thr Thr Asp Thr Ser Thr Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Asp Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Arg Asp Ile Arg Tyr Ser Gly Asn Ser Tyr Lys 
        115                 120                 125             


Trp Tyr Phe Asp Val Trp Gly Gln Gly Thr Met Val Thr Val Ser Ser 
    130                 135                 140                 


<210>  64
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR1 of Humanized 2G1 VH-11801

<400>  64

Gly Tyr Thr Ile Thr Asp Tyr Asn Leu Asp 
1               5                   10  


<210>  65
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR2 of Humanized 2G1 VH-11801

<400>  65

Gly Asp Ile Ser Pro Asn Tyr Gly Tyr Thr Ile Tyr Asn Gln Lys Phe 
1               5                   10                  15      


Lys Asp 
        


<210>  66
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR3 of Humanized 2G1 VH-11801

<400>  66

Arg Asp Ile Arg Tyr Ser Gly Asn Ser Tyr Lys Trp Tyr Phe Asp Val 
1               5                   10                  15      


<210>  67
<211>  432
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of Humanized 2G1 VH-16901

<400>  67
atggactgga cctgggtctt tctcttcctc ctgtcagtaa ctgcaggtgt ccactcccag       60

gtgcagctgg tgcagtctgg ggctgaggtg aagaagcctg ggtcctcggt gaaggtctcc      120

tgcaaggctt ctggatacac aatcacagac tacaatttgg actgggtgcg acaggcccct      180

ggacaagggc ttgagtggat gggagatatt agtcctaact atggttatac tatctacaac      240

cagaaattca aggacagagt cacgattacc gcggacgaat ccacgagcac agcctacatg      300

gagctgagca gcctgagatc tgaggacacg gccgtgtatt actgtgcgag aagggacatt      360

cgttactccg gtaattccta caaatggtac ttcgatgtct ggggccaagg gacaatggtc      420

accgtctctt ca                                                          432


<210>  68
<211>  144
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of Humanized 2G1 VH-16901

<400>  68

Met Asp Trp Thr Trp Val Phe Leu Phe Leu Leu Ser Val Thr Ala Gly 
1               5                   10                  15      


Val His Ser Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys 
            20                  25                  30          


Pro Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Ile 
        35                  40                  45              


Thr Asp Tyr Asn Leu Asp Trp Val Arg Gln Ala Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Met Gly Asp Ile Ser Pro Asn Tyr Gly Tyr Thr Ile Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Lys Asp Arg Val Thr Ile Thr Ala Asp Glu Ser Thr Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Arg Asp Ile Arg Tyr Ser Gly Asn Ser Tyr Lys 
        115                 120                 125             


Trp Tyr Phe Asp Val Trp Gly Gln Gly Thr Met Val Thr Val Ser Ser 
    130                 135                 140                 


<210>  69
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR1 of Humanized 2G1 VH-16901

<400>  69

Gly Tyr Thr Ile Thr Asp Tyr Asn Leu Asp 
1               5                   10  


<210>  70
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR2 of Humanized 2G1 VH-16901

<400>  70

Asp Ile Ser Pro Asn Tyr Gly Tyr Thr Ile Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Asp 
    


<210>  71
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR3 of Humanized 2G1 VH-16901

<400>  71

Arg Asp Ile Arg Tyr Ser Gly Asn Ser Tyr Lys Trp Tyr Phe Asp Val 
1               5                   10                  15      


<210>  72
<211>  381
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of Humanized 2G1 VL-1901

<400>  72
atggaagccc cagctcagct tctcttcctc ctgctactct ggctcccaga taccaccgga       60

gacatccagt tgacccagtc tccatccttc ctgtctgcat ctgtaggaga cagagtcacc      120

atcacttgca ggacaagtaa gagcataagc aaatatttag cctggtatca gcaaaaacca      180

gggaaagccc ctaagctcct gatctattct ggatccacct tgcaatctgg ggtcccatca      240

aggttcagcg gcagtggatc tgggacagaa ttcactctca caatcagcag cctgcagcct      300

gaagattttg caacttatta ctgtcaacaa cataatgaat acccgtacac gtttggccag      360

gggaccaagc tggagatcaa a                                                381


<210>  73
<211>  127
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of Humanized 2G1 VL-1901

<400>  73

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      


Asp Thr Thr Gly Asp Ile Gln Leu Thr Gln Ser Pro Ser Phe Leu Ser 
            20                  25                  30          


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Thr Ser Lys Ser 
        35                  40                  45              


Ile Ser Lys Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
    50                  55                  60                  


Lys Leu Leu Ile Tyr Ser Gly Ser Thr Leu Gln Ser Gly Val Pro Ser 
65                  70                  75                  80  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser 
                85                  90                  95      


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Asn 
            100                 105                 110         


Glu Tyr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
        115                 120                 125         


<210>  74
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR1 of Humanized 2G1 VL-1901

<400>  74

Arg Thr Ser Lys Ser Ile Ser Lys Tyr Leu Ala 
1               5                   10      


<210>  75
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR2 of Humanized 2G1 VL-1901

<400>  75

Ser Gly Ser Thr Leu Gln Ser 
1               5           


<210>  76
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CDR3 of Humanized 2G1 VL-1901

<400>  76

Gln Gln His Asn Glu Tyr Pro Tyr Thr 
1               5                   


<210>  77
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer


<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  A or T

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  A or T

<400>  77
gaggtgaagc tggtggagnc nagg                                              24


<210>  78
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  78
ggggccagtg gatagac                                                      17


<210>  79
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  79
ccagttccga gctccagatg acccagactc ca                                     32


<210>  80
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  80
ccagttccga gctcgtgctc acccagtctc ca                                     32


<210>  81
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  81
ccagttccga gctccagatg acccagtctc ca                                     32


<210>  82
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  82
ccagttccga gctcgtgatg acacagtctc ca                                     32


<210>  83
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  83
gttggtgcag catcagc                                                      17


<210>  84
<211>  104
<212>  PRT
<213>  Homo sapiens

<400>  84

Gln Glu Gln Thr Tyr Val Ile Ser Ala Pro Lys Ile Phe Arg Val Gly 
1               5                   10                  15      


Ala Ser Glu Asn Ile Val Ile Gln Val Tyr Gly Tyr Thr Glu Ala Phe 
            20                  25                  30          


Asp Ala Thr Ile Ser Ile Lys Ser Tyr Pro Asp Lys Lys Phe Ser Tyr 
        35                  40                  45              


Ser Ser Gly His Val His Leu Ser Ser Glu Asn Lys Phe Gln Asn Ser 
    50                  55                  60                  


Ala Ile Leu Thr Ile Gln Pro Lys Gln Leu Pro Gly Gly Gln Asn Pro 
65                  70                  75                  80  


Val Ser Tyr Val Tyr Leu Glu Val Val Ser Lys His Phe Ser Lys Ser 
                85                  90                  95      


Lys Arg Met Pro Ile Thr Tyr Asp 
            100                 


<210>  85
<211>  101
<212>  PRT
<213>  Homo sapiens

<400>  85

Asn Gly Phe Leu Phe Ile His Thr Asp Lys Pro Val Tyr Thr Pro Asp 
1               5                   10                  15      


Gln Ser Val Lys Val Arg Val Tyr Ser Leu Asn Asp Asp Leu Lys Pro 
            20                  25                  30          


Ala Lys Arg Glu Thr Val Leu Thr Phe Ile Asp Pro Glu Gly Ser Glu 
        35                  40                  45              


Val Asp Met Val Glu Glu Ile Asp His Ile Gly Ile Ile Ser Phe Pro 
    50                  55                  60                  


Asp Phe Lys Ile Pro Ser Asn Pro Arg Tyr Gly Met Trp Thr Ile Lys 
65                  70                  75                  80  


Ala Lys Tyr Lys Glu Asp Phe Ser Thr Thr Gly Thr Ala Tyr Phe Glu 
                85                  90                  95      


Val Lys Glu Tyr Val 
            100     


<210>  86
<211>  127
<212>  PRT
<213>  Homo sapiens

<400>  86

Leu Pro His Phe Ser Val Ser Ile Glu Pro Glu Tyr Asn Phe Ile Gly 
1               5                   10                  15      


Tyr Lys Asn Phe Lys Asn Phe Glu Ile Thr Ile Lys Ala Arg Tyr Phe 
            20                  25                  30          


Tyr Asn Lys Val Val Thr Glu Ala Asp Val Tyr Ile Thr Phe Gly Ile 
        35                  40                  45              


Arg Glu Asp Leu Lys Asp Asp Gln Lys Glu Met Met Gln Thr Ala Met 
    50                  55                  60                  


Gln Asn Thr Met Leu Ile Asn Gly Ile Ala Gln Val Thr Phe Asp Ser 
65                  70                  75                  80  


Glu Thr Ala Val Lys Glu Leu Ser Tyr Tyr Ser Leu Glu Asp Leu Asn 
                85                  90                  95      


Asn Lys Tyr Leu Tyr Ile Ala Val Thr Val Ile Glu Ser Thr Gly Gly 
            100                 105                 110         


Phe Ser Glu Glu Ala Glu Ile Pro Gly Ile Lys Tyr Val Leu Ser 
        115                 120                 125         


<210>  87
<211>  108
<212>  PRT
<213>  Homo sapiens

<400>  87

Pro Tyr Lys Leu Asn Leu Val Ala Thr Pro Leu Phe Leu Lys Pro Gly 
1               5                   10                  15      


Ile Pro Tyr Pro Ile Lys Val Gln Val Lys Asp Ser Leu Asp Gln Leu 
            20                  25                  30          


Val Gly Gly Val Pro Val Thr Leu Asn Ala Gln Thr Ile Asp Val Asn 
        35                  40                  45              


Gln Glu Thr Ser Asp Leu Asp Pro Ser Lys Ser Val Thr Arg Val Asp 
    50                  55                  60                  


Asp Gly Val Ala Ser Phe Val Leu Asn Leu Pro Ser Gly Val Thr Val 
65                  70                  75                  80  


Leu Glu Phe Asn Val Lys Thr Asp Ala Pro Asp Leu Pro Glu Glu Asn 
                85                  90                  95      


Gln Ala Arg Glu Gly Tyr Arg Ala Ile Ala Tyr Ser 
            100                 105             


<210>  88
<211>  108
<212>  PRT
<213>  Homo sapiens

<400>  88

Ser Leu Ser Gln Ser Tyr Leu Tyr Ile Asp Trp Thr Asp Asn His Lys 
1               5                   10                  15      


Ala Leu Leu Val Gly Glu His Leu Asn Ile Ile Val Thr Pro Lys Ser 
            20                  25                  30          


Pro Tyr Ile Asp Lys Ile Thr His Tyr Asn Tyr Leu Ile Leu Ser Lys 
        35                  40                  45              


Gly Lys Ile Ile His Phe Gly Thr Arg Glu Lys Phe Ser Asp Ala Ser 
    50                  55                  60                  


Tyr Gln Ser Ile Asn Ile Pro Val Thr Gln Asn Met Val Pro Ser Ser 
65                  70                  75                  80  


Arg Leu Leu Val Tyr Tyr Ile Val Thr Gly Glu Gln Thr Ala Glu Leu 
                85                  90                  95      


Val Ser Asp Ser Val Trp Leu Asn Ile Glu Glu Lys 
            100                 105             


<210>  89
<211>  107
<212>  PRT
<213>  Homo sapiens

<400>  89

Cys Gly Asn Gln Leu Gln Val His Leu Ser Pro Asp Ala Asp Ala Tyr 
1               5                   10                  15      


Ser Pro Gly Gln Thr Val Ser Leu Asn Met Ala Thr Gly Met Asp Ser 
            20                  25                  30          


Trp Val Ala Leu Ala Ala Val Asp Ser Ala Val Tyr Gly Val Gln Arg 
        35                  40                  45              


Gly Ala Lys Lys Pro Leu Glu Arg Val Phe Gln Phe Leu Glu Lys Ser 
    50                  55                  60                  


Asp Leu Gly Cys Gly Ala Gly Gly Gly Leu Asn Asn Ala Asn Val Phe 
65                  70                  75                  80  


His Leu Ala Gly Leu Thr Phe Leu Thr Asn Ala Asn Ala Asp Asp Ser 
                85                  90                  95      


Gln Glu Asn Asp Glu Pro Cys Lys Glu Ile Leu 
            100                 105         


<210>  90
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  90
tccccaggtt ttccccagga agat                                              24


<210>  91
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  91
aatggatttc tcttcattca tac                                               23


<210>  92
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  92
gtcataggtt attggcattc tt                                                22


<210>  93
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  93
ttgccacatt tttctgtctc aatc                                              24


<210>  94
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  94
gacatattct ttaacttcaa aatatg                                            26


<210>  95
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  95
ccctacaaac tgaatttggt tg                                                22


<210>  96
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  96
agagaggaca tatttgatgc cag                                               23


<210>  97
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  97
tctctcagcc aaagttacct t                                                 21


<210>  98
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  98
tgagtatgct attgctcggt aac                                               23


<210>  99
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  99
tgtggcaacc agctccaggt tc                                                22


<210>  100
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  100
tttttcttca atatttaacc ag                                                22


<210>  101
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  101
aggccaagaa gaacgctgca aaag                                              24


<210>  102
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  102
ttttggcaaa gaattcgcca ccatgggcct tttgggaata c                           41


<210>  103
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  103
cctgaggagt gaattcttaa tggtgatggt gatggtggag aatttcttta caaggttc         58


<210>  104
<211>  327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of human IgG4 Constant Heavy chain region

<400>  104

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Pro Gly Lys 
                325         


