                         SEQUENCE LISTING

<110>  Danisco US Inc.
 
<120>  A NOVEL PENICILLIUM OXALICUM GLUCOAMYLASE (CRC04687, POXGA4) WITH
        BROAD SUBSTRATE SPECIFICITY AND HIGH THERMOSTABILITY FOR 
       SACCHARIFICATION AT HIGHER TEMPERATURE, ENHANCED COLD COOK 
       FERMENTATION, AND LOW PH FERMENTATION PROCESSES

<130>  NB40932-WO-PCT[2]

<140>  PCT/US17/60744
<141>  2017-11-09

<150>  PCT/CN2017/075872
<151>  2017-03-17

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  1842
<212>  DNA
<213>  Penicillium russellii

<400>  1
atgcgctata ctcttttaac gagtattgcc agcgtcctta gcgtggggcc gctggcatct       60

gcgagcccta catcgaagga tggaaatttg gcatcctata tagcgaaaga gggacaacga      120

tctattgtgg ggatcactga aaacctcggt ggtaaaggca gcaagacacc gggcacggcc      180

gctggcttat tcattgctag tcctaatatg gcgaacccca attactatta tacgtggact      240

cgtgattcgg cccttacatt caaatgcttg atcgacctgt ttgaaacttc tgatcaggac      300

tatatcagtc gtaaagagtt ggaaaccgac attcggaatt atgtctcatc acaagcagtg      360

ctgcagaatg tatccaatcc atctgggact ctgaaagatg gctctggact cggtgagccc      420

aagtttgaga tcgacctgaa cccattcagt ggttcctggg gccggcctca gcgtgacggt      480

cccgccctgc gagcgaccgc catgatcact tacgcagact ggttggtttc ccacggtcag      540

aaatccgaag ccaccaacat catgtggccc atcattgcaa atgacttggc atatgtaggc      600

cagtactgga acaaaactgg attcgacctc tgggaagagg tagatggatc ttccttttac      660

accttggctg tccaacaccg agcactggtc cagggagcaa gccttgcgaa gaagcttggc      720

aagtcgtgca ctgcttgtgt atcccaagca cctcagattc tttgctttct ccagagcttc      780

tggaacggaa attacatcac cgccaacatc aatcttgaca caagtcgctc cggtatcgat      840

ttgaactcga tcttgggaag catccacacc tttgatcccg aagcttcatg tgatgattcg      900

accttccaac cttgctcagc cagagcgctt gcaaaccata aggtctatgt tgacgcgttc      960

cgttcaatct atggtgtcaa tgctggcctt tcaaacggga cagccgccaa tgtcggtcgg     1020

tacccagaag atgtttacca aggaggtaac ccatggtatc tcgccactct agcagcagct     1080

gaactgctgt acgatgctct ataccaatgg aaccaaattg gcaagctcga tgtcaccaag     1140

acctcgctag cgttcttcaa agattttgac gcggccgtca aaacaggcac atactcagcg     1200

catagctcag cctacaggac tctcacctca gccatccgaa cttatgcaga tgatttcatc     1260

agtctcgtcc aacactatac tccttctaat gggtccttgg ccgagcaata tgatcgggat     1320

actggtatcc cactgtcagc caatgatcta acttggtctt acgcttcctt catcacagct     1380

atcgagcgtc gtgcttccgt cgtgcccgcc tcatggggcg aaaaatctgc aaatgtggtt     1440

cccactacct gctcagcctc tcctgtcact ggaacttacg tagctgctac ttcagtattc     1500

ccgacaacca ctggatgtgt tccggcgaca agcatcgttc cgatcacatt ctacttgact     1560

gagagcacat tctatggaga aaatgtctac atgactggca acattagtgc acttggcaac     1620

tgggacacga gcagtggttt ccccctcaca gcaaacttat acactgattc agaccatttg     1680

tggtttgcca gtgttgagct tgttccggcg gggacaccgt ttgaatacaa atactacaag     1740

gtagagccga atggtactgt catttgggag aacggtgaga acagggtata cgttgctcct     1800

actgggtgtc cgatccagcc tagccaaacc gatatttggc gt                        1842


<210>  2
<211>  614
<212>  PRT
<213>  Penicillium russellii

<400>  2

Met Arg Tyr Thr Leu Leu Thr Ser Ile Ala Ser Val Leu Ser Val Gly 
1               5                   10                  15      


Pro Leu Ala Ser Ala Ser Pro Thr Ser Lys Asp Gly Asn Leu Ala Ser 
            20                  25                  30          


Tyr Ile Ala Lys Glu Gly Gln Arg Ser Ile Val Gly Ile Thr Glu Asn 
        35                  40                  45              


Leu Gly Gly Lys Gly Ser Lys Thr Pro Gly Thr Ala Ala Gly Leu Phe 
    50                  55                  60                  


Ile Ala Ser Pro Asn Met Ala Asn Pro Asn Tyr Tyr Tyr Thr Trp Thr 
65                  70                  75                  80  


Arg Asp Ser Ala Leu Thr Phe Lys Cys Leu Ile Asp Leu Phe Glu Thr 
                85                  90                  95      


Ser Asp Gln Asp Tyr Ile Ser Arg Lys Glu Leu Glu Thr Asp Ile Arg 
            100                 105                 110         


Asn Tyr Val Ser Ser Gln Ala Val Leu Gln Asn Val Ser Asn Pro Ser 
        115                 120                 125             


Gly Thr Leu Lys Asp Gly Ser Gly Leu Gly Glu Pro Lys Phe Glu Ile 
    130                 135                 140                 


Asp Leu Asn Pro Phe Ser Gly Ser Trp Gly Arg Pro Gln Arg Asp Gly 
145                 150                 155                 160 


Pro Ala Leu Arg Ala Thr Ala Met Ile Thr Tyr Ala Asp Trp Leu Val 
                165                 170                 175     


Ser His Gly Gln Lys Ser Glu Ala Thr Asn Ile Met Trp Pro Ile Ile 
            180                 185                 190         


Ala Asn Asp Leu Ala Tyr Val Gly Gln Tyr Trp Asn Lys Thr Gly Phe 
        195                 200                 205             


Asp Leu Trp Glu Glu Val Asp Gly Ser Ser Phe Tyr Thr Leu Ala Val 
    210                 215                 220                 


Gln His Arg Ala Leu Val Gln Gly Ala Ser Leu Ala Lys Lys Leu Gly 
225                 230                 235                 240 


Lys Ser Cys Thr Ala Cys Val Ser Gln Ala Pro Gln Ile Leu Cys Phe 
                245                 250                 255     


Leu Gln Ser Phe Trp Asn Gly Asn Tyr Ile Thr Ala Asn Ile Asn Leu 
            260                 265                 270         


Asp Thr Ser Arg Ser Gly Ile Asp Leu Asn Ser Ile Leu Gly Ser Ile 
        275                 280                 285             


His Thr Phe Asp Pro Glu Ala Ser Cys Asp Asp Ser Thr Phe Gln Pro 
    290                 295                 300                 


Cys Ser Ala Arg Ala Leu Ala Asn His Lys Val Tyr Val Asp Ala Phe 
305                 310                 315                 320 


Arg Ser Ile Tyr Gly Val Asn Ala Gly Leu Ser Asn Gly Thr Ala Ala 
                325                 330                 335     


Asn Val Gly Arg Tyr Pro Glu Asp Val Tyr Gln Gly Gly Asn Pro Trp 
            340                 345                 350         


Tyr Leu Ala Thr Leu Ala Ala Ala Glu Leu Leu Tyr Asp Ala Leu Tyr 
        355                 360                 365             


Gln Trp Asn Gln Ile Gly Lys Leu Asp Val Thr Lys Thr Ser Leu Ala 
    370                 375                 380                 


Phe Phe Lys Asp Phe Asp Ala Ala Val Lys Thr Gly Thr Tyr Ser Ala 
385                 390                 395                 400 


His Ser Ser Ala Tyr Arg Thr Leu Thr Ser Ala Ile Arg Thr Tyr Ala 
                405                 410                 415     


Asp Asp Phe Ile Ser Leu Val Gln His Tyr Thr Pro Ser Asn Gly Ser 
            420                 425                 430         


Leu Ala Glu Gln Tyr Asp Arg Asp Thr Gly Ile Pro Leu Ser Ala Asn 
        435                 440                 445             


Asp Leu Thr Trp Ser Tyr Ala Ser Phe Ile Thr Ala Ile Glu Arg Arg 
    450                 455                 460                 


Ala Ser Val Val Pro Ala Ser Trp Gly Glu Lys Ser Ala Asn Val Val 
465                 470                 475                 480 


Pro Thr Thr Cys Ser Ala Ser Pro Val Thr Gly Thr Tyr Val Ala Ala 
                485                 490                 495     


Thr Ser Val Phe Pro Thr Thr Thr Gly Cys Val Pro Ala Thr Ser Ile 
            500                 505                 510         


Val Pro Ile Thr Phe Tyr Leu Thr Glu Ser Thr Phe Tyr Gly Glu Asn 
        515                 520                 525             


Val Tyr Met Thr Gly Asn Ile Ser Ala Leu Gly Asn Trp Asp Thr Ser 
    530                 535                 540                 


Ser Gly Phe Pro Leu Thr Ala Asn Leu Tyr Thr Asp Ser Asp His Leu 
545                 550                 555                 560 


Trp Phe Ala Ser Val Glu Leu Val Pro Ala Gly Thr Pro Phe Glu Tyr 
                565                 570                 575     


Lys Tyr Tyr Lys Val Glu Pro Asn Gly Thr Val Ile Trp Glu Asn Gly 
            580                 585                 590         


Glu Asn Arg Val Tyr Val Ala Pro Thr Gly Cys Pro Ile Gln Pro Ser 
        595                 600                 605             


Gln Thr Asp Ile Trp Arg 
    610                 


<210>  3
<211>  590
<212>  PRT
<213>  Penicillium russellii

<400>  3

Ser Lys Asp Gly Asn Leu Ala Ser Tyr Ile Ala Lys Glu Gly Gln Arg 
1               5                   10                  15      


Ser Ile Val Gly Ile Thr Glu Asn Leu Gly Gly Lys Gly Ser Lys Thr 
            20                  25                  30          


Pro Gly Thr Ala Ala Gly Leu Phe Ile Ala Ser Pro Asn Met Ala Asn 
        35                  40                  45              


Pro Asn Tyr Tyr Tyr Thr Trp Thr Arg Asp Ser Ala Leu Thr Phe Lys 
    50                  55                  60                  


Cys Leu Ile Asp Leu Phe Glu Thr Ser Asp Gln Asp Tyr Ile Ser Arg 
65                  70                  75                  80  


Lys Glu Leu Glu Thr Asp Ile Arg Asn Tyr Val Ser Ser Gln Ala Val 
                85                  90                  95      


Leu Gln Asn Val Ser Asn Pro Ser Gly Thr Leu Lys Asp Gly Ser Gly 
            100                 105                 110         


Leu Gly Glu Pro Lys Phe Glu Ile Asp Leu Asn Pro Phe Ser Gly Ser 
        115                 120                 125             


Trp Gly Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala Thr Ala Met 
    130                 135                 140                 


Ile Thr Tyr Ala Asp Trp Leu Val Ser His Gly Gln Lys Ser Glu Ala 
145                 150                 155                 160 


Thr Asn Ile Met Trp Pro Ile Ile Ala Asn Asp Leu Ala Tyr Val Gly 
                165                 170                 175     


Gln Tyr Trp Asn Lys Thr Gly Phe Asp Leu Trp Glu Glu Val Asp Gly 
            180                 185                 190         


Ser Ser Phe Tyr Thr Leu Ala Val Gln His Arg Ala Leu Val Gln Gly 
        195                 200                 205             


Ala Ser Leu Ala Lys Lys Leu Gly Lys Ser Cys Thr Ala Cys Val Ser 
    210                 215                 220                 


Gln Ala Pro Gln Ile Leu Cys Phe Leu Gln Ser Phe Trp Asn Gly Asn 
225                 230                 235                 240 


Tyr Ile Thr Ala Asn Ile Asn Leu Asp Thr Ser Arg Ser Gly Ile Asp 
                245                 250                 255     


Leu Asn Ser Ile Leu Gly Ser Ile His Thr Phe Asp Pro Glu Ala Ser 
            260                 265                 270         


Cys Asp Asp Ser Thr Phe Gln Pro Cys Ser Ala Arg Ala Leu Ala Asn 
        275                 280                 285             


His Lys Val Tyr Val Asp Ala Phe Arg Ser Ile Tyr Gly Val Asn Ala 
    290                 295                 300                 


Gly Leu Ser Asn Gly Thr Ala Ala Asn Val Gly Arg Tyr Pro Glu Asp 
305                 310                 315                 320 


Val Tyr Gln Gly Gly Asn Pro Trp Tyr Leu Ala Thr Leu Ala Ala Ala 
                325                 330                 335     


Glu Leu Leu Tyr Asp Ala Leu Tyr Gln Trp Asn Gln Ile Gly Lys Leu 
            340                 345                 350         


Asp Val Thr Lys Thr Ser Leu Ala Phe Phe Lys Asp Phe Asp Ala Ala 
        355                 360                 365             


Val Lys Thr Gly Thr Tyr Ser Ala His Ser Ser Ala Tyr Arg Thr Leu 
    370                 375                 380                 


Thr Ser Ala Ile Arg Thr Tyr Ala Asp Asp Phe Ile Ser Leu Val Gln 
385                 390                 395                 400 


His Tyr Thr Pro Ser Asn Gly Ser Leu Ala Glu Gln Tyr Asp Arg Asp 
                405                 410                 415     


Thr Gly Ile Pro Leu Ser Ala Asn Asp Leu Thr Trp Ser Tyr Ala Ser 
            420                 425                 430         


Phe Ile Thr Ala Ile Glu Arg Arg Ala Ser Val Val Pro Ala Ser Trp 
        435                 440                 445             


Gly Glu Lys Ser Ala Asn Val Val Pro Thr Thr Cys Ser Ala Ser Pro 
    450                 455                 460                 


Val Thr Gly Thr Tyr Val Ala Ala Thr Ser Val Phe Pro Thr Thr Thr 
465                 470                 475                 480 


Gly Cys Val Pro Ala Thr Ser Ile Val Pro Ile Thr Phe Tyr Leu Thr 
                485                 490                 495     


Glu Ser Thr Phe Tyr Gly Glu Asn Val Tyr Met Thr Gly Asn Ile Ser 
            500                 505                 510         


Ala Leu Gly Asn Trp Asp Thr Ser Ser Gly Phe Pro Leu Thr Ala Asn 
        515                 520                 525             


Leu Tyr Thr Asp Ser Asp His Leu Trp Phe Ala Ser Val Glu Leu Val 
    530                 535                 540                 


Pro Ala Gly Thr Pro Phe Glu Tyr Lys Tyr Tyr Lys Val Glu Pro Asn 
545                 550                 555                 560 


Gly Thr Val Ile Trp Glu Asn Gly Glu Asn Arg Val Tyr Val Ala Pro 
                565                 570                 575     


Thr Gly Cys Pro Ile Gln Pro Ser Gln Thr Asp Ile Trp Arg 
            580                 585                 590 


<210>  4
<211>  1845
<212>  DNA
<213>  Penicillium russellii

<400>  4
atgcgctata ctcttttaac gagtattgcc agcgtcctta gcgtggggcc gctggcatct       60

gcgagcccta catcgaagga tggaaatttg gcatcctata tagcgaaaga gggacaacga      120

tctattgtgg ggatcactga aaacctcggt ggtaaaggca gcaagacacc gggcacggcc      180

gctggcttat tcattgctag tcctaatatg gcgaacccca attactatta tacgtggact      240

cgtgattcgg cccttacatt caaatgcttg atcgacctgt ttgaaacttc tgatcaggac      300

tatatcagtc gtaaagagtt ggaaaccgac attcggaatt atgtctcatc acaagcagtg      360

ctgcagaatg tatccaatcc atctgggact ctgaaagatg gctctggact cggtgagccc      420

aagtttgaga tcgacctgaa cccattcagt ggttcctggg gccggcctca gcgtgacggt      480

cccgccctgc gagcgaccgc catgatcact tacgcagact ggttggtttc ccacggtcag      540

aaatccgaag ccaccaacat catgtggccc atcattgcaa atgacttggc atatgtaggc      600

cagtactgga acaaaactgg attcgacctc tgggaagagg tagatggatc ttccttttac      660

accttggctg tccaacaccg agcactggtc cagggagcaa gccttgcgaa gaagcttggc      720

aagtcgtgca ctgcttgtgt atcccaagca cctcagattc tttgctttct ccagagcttc      780

tggaacggaa attacatcac cgccaacatc aatcttgaca caagtcgctc cggtatcgat      840

ttgaactcga tcttgggaag catccacacc tttgatcccg aagcttcatg tgatgattcg      900

accttccaac cttgctcagc cagagcgctt gcaaaccata aggtctatgt tgacgcgttc      960

cgttcaatct atggtgtcaa tgctggcctt tcaaacggga cagccgccaa tgtcggtcgg     1020

tacccagaag atgtttacca aggaggtaac ccatggtatc tcgccactct agcagcagct     1080

gaactgctgt acgatgctct ataccaatgg aaccaaattg gcaagctcga tgtcaccaag     1140

acctcgctag cgttcttcaa agattttgac gcggccgtca aaacaggcac atactcagcg     1200

catagctcag cctacaggac tctcacctca gccatccgaa cttatgcaga tgatttcatc     1260

agtctcgtcc aacactatac tccttctaat gggtccttgg ccgagcaata tgatcgggat     1320

actggtatcc cactgtcagc caatgatcta acttggtctt acgcttcctt catcacagct     1380

atcgagcgtc gtgcttccgt cgtgcccgcc tcatggggcg aaaaatctgc aaatgtggtt     1440

cccactacct gctcagcctc tcctgtcact ggaacttacg tagctgctac ttcagtattc     1500

ccgacaacca ctggatgtgt tccggcgaca agcatcgttc cgatcacatt ctacttgact     1560

gagagcacat tctatggaga aaatgtctac atgactggca acattagtgc acttggcaac     1620

tgggacacga gcagtggttt ccccctcaca gcaaacttat acactgattc agaccatttg     1680

tggtttgcca gtgttgagct tgttccggcg gggacaccgt ttgaatacaa atactacaag     1740

gtagagccga atggtactgt catttgggag aacggtgaga acagggtata cgttgctcct     1800

actgggtgtc cgatccagcc tagccaaacc gatatttggc gttaa                     1845


