                         SEQUENCE LISTING

<110>  Yale University
       Massachusetts Institute of Technology
       Chen, Sidi
       Platt, Randall  J
       Chow, Ryan
       Guzman, Christopher
 
<120>  AAV-Mediated Direct In vivo CRISPR Screen in Glioblastoma

<130>  047162-7116WO1(00690)

<150>  62/600,793
<151>  2017-03-03

<160>  290   

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  1
gggcagtgtt tcaaaatcca                                                   20


<210>  2
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<212>  DNA
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<223>  sgRNA

<400>  2
ggaacacgag ccgtcagcgg                                                   20


<210>  3
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<400>  3
ggatttctgt tctgcatcaa                                                   20


<210>  4
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<400>  4
ggtcccatct gttgcctcca                                                   20


<210>  5
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<400>  5
gtttggagat ccactcgata                                                   20


<210>  6
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<223>  sgRNA

<400>  6
gtacccagtg caagctacag                                                   20


<210>  7
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<223>  sgRNA

<400>  7
gggtaccccg ctatatgttg                                                   20


<210>  8
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<212>  DNA
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<220>
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<400>  8
gcatctggcc cccaggagat                                                   20


<210>  9
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  9
ggtccgtact gagacatctg                                                   20


<210>  10
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  10
gattctgact ggcttccagg                                                   20


<210>  11
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  11
gtgaaatact ctccatcaag                                                   20


<210>  12
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<212>  DNA
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<223>  sgRNA

<400>  12
gggagaggcg cttgtgcagg                                                   20


<210>  13
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<212>  DNA
<213>  Artificial sequence

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<400>  13
gcaaagagag gtacgcaggc                                                   20


<210>  14
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  14
gcggttcatg ccccccatgc                                                   20


<210>  15
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<212>  DNA
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<223>  sgRNA

<400>  15
ggtatactca gagccggcct                                                   20


<210>  16
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<212>  DNA
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<400>  16
gccatggctg aaggggagcc                                                   20


<210>  17
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  17
gactctgtag gtgacagcaa                                                   20


<210>  18
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  18
gctcctaaac ctacttgttc                                                   20


<210>  19
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  19
gcatgctgta tggtaccagg                                                   20


<210>  20
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<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  20
gaaggcatct accgggtcag                                                   20


<210>  21
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  21
ggacgatgat gaggacgatg                                                   20


<210>  22
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  22
gtgtaatttc aaagccccct                                                   20


<210>  23
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  23
gtggacagca tctagtaggt                                                   20


<210>  24
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<212>  DNA
<213>  Artificial sequence

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<400>  24
gggcggttct cttcccaaag                                                   20


<210>  25
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  25
ggaagagaac cgccctattg                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  26
gcatatgctc gtaaagtgga                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  27
gatgagtgaa aatgctggtg                                                   20


<210>  28
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  28
ggctgagctg ctgttggcaa                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  29
gccctaggtg ctagtcctat                                                   20


<210>  30
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  30
gctagtccta tgggtgtaaa                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  31
gccggctatg tcgggcctgt                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  32
gggattcagt tctgctgagc                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  33
gtgtgtgaga catgtgacaa                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  34
gcaggcaggt cctccatagg                                                   20


<210>  35
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  35
gcctcctctg ccggagaaag                                                   20


<210>  36
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  36
gactgtgaat ggacagctga                                                   20


<210>  37
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  37
gtcttctcaa aacatttcag                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  38
gcactgatgg aaaatggtga                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  39
gactaccagt tccaaagata                                                   20


<210>  40
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  40
gaagcttctg gttactttcc                                                   20


<210>  41
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  41
gggattggcc gcgaagttcc                                                   20


<210>  42
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  42
ggggtacgac cgaaagagtt                                                   20


<210>  43
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  43
gggtcgcctg ccgctcgact                                                   20


<210>  44
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  44
gggaacgtcg cccagaccga                                                   20


<210>  45
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  45
ggggtacgac cgaaagagtt                                                   20


<210>  46
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  46
gtacctgcac caggaaaacc                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  47
gtggagccat acattgcatg                                                   20


<210>  48
<211>  20
<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  48
gggtgagttt tctgtctagt                                                   20


<210>  49
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  49
gcctttgtca tcagaattcg                                                   20


<210>  50
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  50
gaaggcaaag cactatcaca                                                   20


<210>  51
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  51
gcaatggtct tgagatctat                                                   20


<210>  52
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  52
gaccattgct cagaggatac                                                   20


<210>  53
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  53
gcctgggtct caagtattca                                                   20


<210>  54
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  54
gccaaaacat acaatgagcc                                                   20


<210>  55
<211>  20
<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  55
gtgcgaagga cctgtcagcc                                                   20


<210>  56
<211>  20
<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  56
gactgcatgg gcagaaggga                                                   20


<210>  57
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  57
gagacggcac tttccttgtc                                                   20


<210>  58
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  58
gttggctaca gtagtgggct                                                   20


<210>  59
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  59
ggcagtgctg caggcaaaag                                                   20


<210>  60
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  60
ggctgacgca gaaaggtgtg                                                   20


<210>  61
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  61
ggccgcaagc tgacgcctca                                                   20


<210>  62
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  62
gcctcaggga cagagagacc                                                   20


<210>  63
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  63
gtccctgagg cgtcagcttg                                                   20


<210>  64
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  64
gggccgcaag ctgacgcctc                                                   20


<210>  65
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  65
gttgaaacag agcggggggg                                                   20


<210>  66
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  66
gtggatgaaa ggctcttcat                                                   20


<210>  67
<211>  20
<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  67
ggttttgcac agtctcttcc                                                   20


<210>  68
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  68
gacctcaggc tgaacagcct                                                   20


<210>  69
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  69
ggcccaggct gttcagcctg                                                   20


<210>  70
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  70
gtccaccacc ccctggtcac                                                   20


<210>  71
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  71
gttggcactg atttcataac                                                   20


<210>  72
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<212>  DNA
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<223>  sgRNA

<400>  72
gggagaagat agcaagatgc                                                   20


<210>  73
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<212>  DNA
<213>  Artificial sequence

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<400>  73
gtggctactg accaaaccca                                                   20


<210>  74
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<212>  DNA
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<223>  sgRNA

<400>  74
gagaattcct aacagctatg                                                   20


<210>  75
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  75
gctgccgata ctccaaactt                                                   20


<210>  76
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<212>  DNA
<213>  Artificial sequence

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<400>  76
gcaactattt tacaacaatt                                                   20


<210>  77
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<212>  DNA
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<400>  77
ggtaaattaa aacactcacc                                                   20


<210>  78
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  78
gtaaattaaa acactcacct                                                   20


<210>  79
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<212>  DNA
<213>  Artificial sequence

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<400>  79
gcagcatttt cagttagctt                                                   20


<210>  80
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  80
ggctattaaa gcatttcagg                                                   20


<210>  81
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<212>  DNA
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<400>  81
gtgattttga tctcgtgcct                                                   20


<210>  82
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<212>  DNA
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<400>  82
gcaaggtaca ctgtaatcag                                                   20


<210>  83
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<212>  DNA
<213>  Artificial sequence

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<400>  83
gtgcttatga atccatgaaa                                                   20


<210>  84
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<212>  DNA
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<400>  84
gtccaaatat atagtaaggt                                                   20


<210>  85
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<212>  DNA
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<223>  sgRNA

<400>  85
gagacttgag gaaaatgtta                                                   20


<210>  86
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  86
ggggccaagg gtatgccaga                                                   20


<210>  87
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<212>  DNA
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<400>  87
gactgtggga tcccagtttc                                                   20


<210>  88
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<212>  DNA
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<400>  88
gtaggtagga ggtgaactca                                                   20


<210>  89
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<212>  DNA
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<400>  89
gcatgttcaa catcgtaggt                                                   20


<210>  90
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<212>  DNA
<213>  Artificial sequence

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<400>  90
ggagtcttct gcctggttcc                                                   20


<210>  91
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<212>  DNA
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<400>  91
ggactaccca agtgtgcgga                                                   20


<210>  92
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<212>  DNA
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<400>  92
gcaccttgag agtcagcacc                                                   20


<210>  93
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<212>  DNA
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<400>  93
ggttaaccag aagtccatca                                                   20


<210>  94
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<212>  DNA
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<400>  94
gtgccatccc tcaatgtcga                                                   20


<210>  95
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  95
gtcctgagga gatggaggct                                                   20


<210>  96
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<212>  DNA
<213>  Artificial sequence

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<400>  96
gtctccttct tctagaggca                                                   20


<210>  97
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  97
ggcaaaacag aataggagag                                                   20


<210>  98
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<212>  DNA
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<223>  sgRNA

<400>  98
ggcaatttga tacaagtaac                                                   20


<210>  99
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<212>  DNA
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<400>  99
gcaatttgat acaagtaact                                                   20


<210>  100
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<212>  DNA
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<400>  100
gcacagtatc aaaatacttg                                                   20


<210>  101
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<212>  DNA
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<400>  101
gacaaagttg atgaaactgg                                                   20


<210>  102
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<212>  DNA
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<400>  102
gccgatttcc ttatccaaag                                                   20


<210>  103
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<212>  DNA
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<400>  103
gacccaagtg catcaagaca                                                   20


<210>  104
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<212>  DNA
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<400>  104
gcacttgggt ctattctttc                                                   20


<210>  105
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  105
gggcgactgt tggatctgta                                                   20


<210>  106
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  106
gtccagtaaa agctggagga                                                   20


<210>  107
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<212>  DNA
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<400>  107
gagtggttct gaaatccaca                                                   20


<210>  108
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<212>  DNA
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<400>  108
ggagagcaat gttaagctct                                                   20


<210>  109
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<212>  DNA
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<400>  109
gactgtgtgc agagagcaac                                                   20


<210>  110
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  110
gtcacttctc attacagttt                                                   20


<210>  111
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  111
gatgccctgc agaggaaagg                                                   20


<210>  112
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  112
ggcagagcgc ttcagtgagc                                                   20


<210>  113
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<212>  DNA
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<400>  113
gacagtgtgc tgagagaccg                                                   20


<210>  114
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<212>  DNA
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<400>  114
ggccggaaat tcccagcttc                                                   20


<210>  115
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<212>  DNA
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<400>  115
gtgtttcttt tggtcttagg                                                   20


<210>  116
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<212>  DNA
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<400>  116
gtgtttctcc ctttaagtct                                                   20


<210>  117
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<212>  DNA
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<223>  sgRNA

<400>  117
ggcagcccca attctgctca                                                   20


<210>  118
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<212>  DNA
<213>  Artificial sequence

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<400>  118
gatattagcc gtgactcaga                                                   20


<210>  119
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<212>  DNA
<213>  Artificial sequence

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<400>  119
gaagacaaag atgattttaa                                                   20


<210>  120
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<212>  DNA
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<400>  120
ggtttcctac aaaagagtta                                                   20


<210>  121
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<212>  DNA
<213>  Artificial sequence

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<223>  sgRNA

<400>  121
gctcattggt tggtttctgc                                                   20


<210>  122
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  122
gcctttctcc aaaaggtatg                                                   20


<210>  123
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  123
ggttagtatg gctccgcgtg                                                   20


<210>  124
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  124
gcgttacttc cagattaacc                                                   20


<210>  125
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  125
gcgttacttc cagattaacc                                                   20


<210>  126
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  126
gataaacctc ttaggattac                                                   20


<210>  127
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  127
ggaacgggct ggtgttaaaa                                                   20


<210>  128
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  128
gtcttccctt ttcaacaatc                                                   20


<210>  129
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  129
gaaaagggaa gaccagcccc                                                   20


<210>  130
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  130
gttagcatac aagacctttc                                                   20


<210>  131
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  131
ggaggcggtg agtagtaagg                                                   20


<210>  132
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  132
gaggaggcgg tgagtagtaa                                                   20


<210>  133
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  133
gaattttgga catgctaggg                                                   20


<210>  134
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  134
gtgagcctgt tttctcctct                                                   20


<210>  135
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  135
ggttacctag aggagaaaac                                                   20


<210>  136
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  136
gtatacacct tcataacctg                                                   20


<210>  137
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  137
gcagtggcca ttgtgcagac                                                   20


<210>  138
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  138
ggcacctggt gaaagaggca                                                   20


<210>  139
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  139
gccaaccctt gtgagcacgc                                                   20


<210>  140
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  140
gagcacactc atccacgtcc                                                   20


<210>  141
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  141
ggtgacaagg gtgtcgtcta                                                   20


<210>  142
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  142
gtgtcgtcta tggaacggat                                                   20


<210>  143
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  143
ggtaaacttt gtctgaagtc                                                   20


<210>  144
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  144
ggagttgggt tatgtcttcc                                                   20


<210>  145
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  145
gactcactgt cttgttctct                                                   20


<210>  146
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  146
gagtgtttgt acatagatac                                                   20


<210>  147
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  147
gcagaacgga ataaaatgat                                                   20


<210>  148
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  148
gatgactgct ttggtaaatg                                                   20


<210>  149
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  149
gattacccac ttaccatggc                                                   20


<210>  150
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  150
gaggaccagc catggtaagt                                                   20


<210>  151
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  151
gctctgcaga gtatattccc                                                   20


<210>  152
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  152
gcatgtaggt gatgcagggc                                                   20


<210>  153
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  153
gtttgtcatc ttcatctcct                                                   20


<210>  154
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  154
gtatgccgaa tgtgttcccg                                                   20


<210>  155
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  155
gaccttccta gaaggcaagg                                                   20


<210>  156
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  156
gtccatttca aagtaagcaa                                                   20


<210>  157
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  157
gcaatggagc accagtactc                                                   20


<210>  158
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  158
gatgattgga aatgggaggc                                                   20


<210>  159
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  159
gtcacaacag ggcagcttga                                                   20


<210>  160
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  160
gatggctatg tggatccttc                                                   20


<210>  161
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  161
gggctcccgt gggcacttca                                                   20


<210>  162
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  162
gaaaaccctg aagtgcccac                                                   20


<210>  163
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  163
gaagattccc cgggtgggcc                                                   20


<210>  164
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  164
gggtgggccc ggaacatctc                                                   20


<210>  165
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  165
gattgcgatg cgctcatggc                                                   20


<210>  166
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  166
gcgcgcgggg ggcatgttgg                                                   20


<210>  167
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  167
gcctcctcca ggcgcgcggg                                                   20


<210>  168
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  168
gtcctagtgt agggaccggg                                                   20


<210>  169
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  169
gagggttggg cgtgggggct                                                   20


<210>  170
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  170
gtagaggtgc gtatctgtca                                                   20


<210>  171
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  171
gttcgaggtg aaccattaat                                                   20


<210>  172
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  172
gaggtcagaa caggagcgct                                                   20


<210>  173
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  173
ggctctctga gtagtgcagg                                                   20


<210>  174
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  174
gaatcatgga atcccttgca                                                   20


<210>  175
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  175
gaaccttttt attcctagga                                                   20


<210>  176
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  176
gaggcagaac gtcgtaaaga                                                   20


<210>  177
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  177
gttctcttcc ggcgaggaga                                                   20


<210>  178
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  178
ggaggtggac tcggagtgcg                                                   20


<210>  179
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  179
gagatgggaa ggacagaggc                                                   20


<210>  180
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  180
gactttctca gagaaggtga                                                   20


<210>  181
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  181
gaaccgacaa acagtcctgg                                                   20


<210>  182
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  182
ggtcaggcac cactgccatc                                                   20


<210>  183
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  183
gtcctctccc cagggcccta                                                   20


<210>  184
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  184
gtggacagat aaagctcgaa                                                   20


<210>  185
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  185
gctatgtgcc tatcacaggg                                                   20


<210>  186
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  186
gggataccta cctgaatcca                                                   20


<210>  187
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  187
gggaggtggg ggactccacg                                                   20


<210>  188
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  188
gtcccctttg tagatctaag                                                   20


<210>  189
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  189
ggagatccca tgacttctac                                                   20


<210>  190
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  190
ggggagggga cacctacaga                                                   20


<210>  191
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  191
gagattattc tctgtatttt                                                   20


<210>  192
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  192
gtcttaatgt ctttccttta                                                   20


<210>  193
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  193
gatcttcttc tcggccctaa                                                   20


<210>  194
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  194
gttcacaatg agttagaaga                                                   20


<210>  195
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  195
ggacactgag atatatctat                                                   20


<210>  196
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  196
gtccatggta gttgatctta                                                   20


<210>  197
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  197
gctgcagcca agagctcttg                                                   20


<210>  198
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  198
gattatccga attcttagca                                                   20


<210>  199
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  199
gacaatctga tgctatatct                                                   20


<210>  200
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  200
ggtatatttt ccaagtcttg                                                   20


<210>  201
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  201
gtggagagct gtctcaccag                                                   20


<210>  202
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  202
gggtgtggag gtgtctgatg                                                   20


<210>  203
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  203
ggtcatgcac aggtggcggc                                                   20


<210>  204
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  204
gatggcacag ctctgaagag                                                   20


<210>  205
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  205
gctctggaag tgcaggcttg                                                   20


<210>  206
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  206
ggtggtgagg tccgaaggag                                                   20


<210>  207
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  207
ggaagggtgg tgaggtccga                                                   20


<210>  208
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  208
gcccacaggc attgcagacc                                                   20


<210>  209
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  209
gaggaacgct aatggggacc                                                   20


<210>  210
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  210
gtaccatctc gccgccacag                                                   20


<210>  211
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  211
ggttcattgt cactaacatc                                                   20


<210>  212
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  212
gaatgctgat cttcatcaaa                                                   20


<210>  213
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  213
gaacttgtcc tcccgccgcg                                                   20


<210>  214
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  214
gttcttcata ccaggaccag                                                   20


<210>  215
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  215
gggaattgtg actccctgat                                                   20


<210>  216
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  216
gctgcagaag aaaaagatac                                                   20


<210>  217
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  217
gcgccacttt tgggggtaag                                                   20


<210>  218
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  218
gaacctagat tttgagacag                                                   20


<210>  219
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  219
gaattttctt cagcctctcc                                                   20


<210>  220
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  220
gagggctgcg ccacttttgg                                                   20


<210>  221
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  221
ggctacccaa atatgaatca                                                   20


<210>  222
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  222
ggacccccat atcctatggg                                                   20


<210>  223
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  223
gctgcctagg atagcctcct                                                   20


<210>  224
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  224
gacgcatgag ccattctccc                                                   20


<210>  225
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  225
gaagtgtact ggggcatctg                                                   20


<210>  226
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  226
ggagagagtt tacttccgag                                                   20


<210>  227
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  227
gtctttgtcc tgaggcctta                                                   20


<210>  228
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  228
gtggagtgct gcactggccc                                                   20


<210>  229
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  229
gctgtgagtg aatgatgttg                                                   20


<210>  230
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  230
gccagtgttt tgagttctag                                                   20


<210>  231
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  231
gtctacaagc ataatcacac                                                   20


<210>  232
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  232
gattatgctt gtagacaggt                                                   20


<210>  233
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  233
gatggcgtag agggggaaaa                                                   20


<210>  234
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  234
gataactgtg ctggtccaga                                                   20


<210>  235
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  235
gctatgacag tgtcacaatg                                                   20


<210>  236
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  236
gtacaggcag gaggcaactg                                                   20


<210>  237
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  237
gcaggaggca actggggact                                                   20


<210>  238
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  238
ggggtgcaca gtcttgatgg                                                   20


<210>  239
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  239
gtgtagccgt tctgctccac                                                   20


<210>  240
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  240
gtaccttggc cactagtggg                                                   20


<210>  241
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  241
gtggaacggc acatgtgtga                                                   20


<210>  242
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  242
ggaacggcac atgtgtgatg                                                   20


<210>  243
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  243
gacttcagga attagtacgc                                                   20


<210>  244
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  244
gaaggtcact gggcttagga                                                   20


<210>  245
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  245
gtctgcagat gaaggtcact                                                   20


<210>  246
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  246
gactccttgt ctgaccccac                                                   20


<210>  247
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  247
gaggaccatt gtcatccgcc                                                   20


<210>  248
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  248
ggatgtaatg gagatagtcc                                                   20


<210>  249
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  249
gtcacctgaa acagggggac                                                   20


<210>  250
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  250
gtcggatcct gtctggtgag                                                   20


<210>  251
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  251
ggagcccgag gaggggtttg                                                   20


<210>  252
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  252
gggcgcaggc cttcctggag                                                   20


<210>  253
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  253
gaagaaacac cctctggctg                                                   20


<210>  254
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  254
gtgtctgggc ttggtgggat                                                   20


<210>  255
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  255
gtgctgccta atctgtcgga                                                   20


<210>  256
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  256
gctccacagt gccagcgttc                                                   20


<210>  257
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  257
gcgaagaaga atctaagagg                                                   20


<210>  258
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  258
ggagaagcac tgccgggata                                                   20


<210>  259
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  259
ggttagcgga gcagtgtcca                                                   20


<210>  260
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  260
ggtgctggcg caggagagcc                                                   20


<210>  261
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  261
gaaaacagcc aaggtttgta                                                   20


<210>  262
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  262
gggtcaagtg cctgagaatg                                                   20


<210>  263
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  263
gagttaccct acatacactc                                                   20


<210>  264
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  264
gttcaggctg ctgaccttca                                                   20


<210>  265
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  265
ggaggttcct gtcaaaggag                                                   20


<210>  266
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  266
ggtcttgggc tcggccatac                                                   20


<210>  267
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  267
gggtgaattc agtgtgagcc                                                   20


<210>  268
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  268
gagcccaaga ccgtctactg                                                   20


<210>  269
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  269
gtatgtatca gtctcagtgg                                                   20


<210>  270
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  270
ggtcgcttca gtcgtcagca                                                   20


<210>  271
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  271
gccgcttgca gcaggtcttt                                                   20


<210>  272
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  272
gcagcaggtc tttgggttcc                                                   20


<210>  273
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  273
gagtgtatac atactttata                                                   20


<210>  274
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  274
gtatgcatct ccatgaaaaa                                                   20


<210>  275
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  275
gatctgtaca cttttcttat                                                   20


<210>  276
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  276
gcggggcgca ctgggcagcg                                                   20


<210>  277
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  277
gccaccgctg cccactgaga                                                   20


<210>  278
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  278
gacggcaaac cctgccaggc                                                   20


<210>  279
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  279
gccatgcagc agcacgccgt                                                   20


<210>  280
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  280
gccgtggggg gctactgcaa                                                   20


<210>  281
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  281
acggaggcta agcgtcgcaa                                                   20


<210>  282
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  282
cgcttccgcg gcccgttcaa                                                   20


<210>  283
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  283
atcgtttccg cttaacggcg                                                   20


<210>  284
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  284
gtaggcgcgc cgctctctac                                                   20


<210>  285
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  285
ccatatcggg gcgagacatg                                                   20


<210>  286
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  286
tactaacgcc gctcctacag                                                   20


<210>  287
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  287
tgaggatcat gtcgagcgcc                                                   20


<210>  288
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sgRNA

<400>  288
gggcccgcat aggatatcgc                                                   20


<210>  289
<211>  7006
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Vector

<400>  289
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc       60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca      120

actccatcac taggggttcc tgcggccgca cgcgtgaggg cctatttccc atgattcctt      180

catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa      240

acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg      300

cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt      360

tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgtgtaa tagctcctgc      420

atgggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa      480

aagtggcacc gagtcggtgc tttttttcta gaagagggcc tatttcccat gattccttca      540

tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac      600

acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca      660

gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc      720

gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccggaagagc gagctcttct      780

gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt      840

ggcaccgagt cggtgctttt ttggtaccag gatcccacct ccctctctgt gctgggactc      900

acagagggag acctcaggag gcagtctgtc catcacatgt ccaaatgcag agcataccct      960

gggctgggcg cagtggcgca caactgtaat tccagcactt tgggaggctg atgtggaagg     1020

atcacttgag cccagaagtt ctagaccagc ctgggcaaca tggcaagacc ctatctctac     1080

aaaaaaagtt aaaaaatcag ccacgtgtgg tgacacacac ctgtagtccc agctattcag     1140

gaggctgagg tgaggggatc acttaaggct gggaggttga ggctgcagtg agtcgtggtt     1200

gcgccactgc actccagcct gggcaacagt gagaccctgt ctcaaaagac aaaaaaaaaa     1260

aaaaaaaaaa aaagaacata tcctggtgtg gagtagggga cgctgctctg acagaggctc     1320

gggggcctga gctggctctg tgagctgggg aggaggcaga cagccaggcc ttgtctgcaa     1380

gcagacctgg cagcattggg ctggccgccc cccagggcct cctcttcatg cccagtgaat     1440

gactcacctt ggcacagaca caatgttcgg ggtgggcaca gtgcctgctt cccgccgcac     1500

cccagccccc ctcaaatgcc ttccgagaag cccattgagc agggggcttg cattgcaccc     1560

cagcctgaca gcctggcatc ttgggataaa agcagcacag ccccctaggg gctgcccttg     1620

ctgtgtggcg ccaccggcgg tggagaacaa ggctctattc agcctgtgcc caggaaaggg     1680

gatcagggga tgcccaggca tggacagtgg gtggcagggg gggagaggag ggctgtctgc     1740

ttcccagaag tccaaggaca caaatgggtg aggggactgg gcagggttct gaccctgtgg     1800

gaccagagtg gagggcgtag atggacctga agtctccagg gacaacaggg cccaggtctc     1860

aggctcctag ttgggcccag tggctccagc gtttccaaac ccatccatcc ccagaggttc     1920

ttcccatctc tccaggctga tgtgtgggaa ctcgaggaaa taaatctcca gtgggagacg     1980

gaggggtggc cagggaaacg gggcgctgca ggaataaaga cgagccagca cagccagctc     2040

atgtgtaacg gctttgtgga gctgtcaagg cctggtctct gggagagagg cacagggagg     2100

ccagacaagg aaggggtgac ctggagggac agatccaggg gctaaagtcc tgataaggca     2160

agagagtgcc ggccccctct tgccctatca ggacctccac tgccacatag aggccatgat     2220

tgacccttag acaaagggct ggtgtccaat cccagccccc agccccagaa ctccagggaa     2280

tgaatgggca gagagcagga atgtgggaca tctgtgttca agggaaggac tccaggagtc     2340

tgctgggaat gaggcctagt aggaaatgag gtggcccttg agggtacaga acaggttcat     2400

tcttcgccaa attcccagca ccttgcaggc acttacagct gagtgagata atgcctgggt     2460

tatgaaatca aaaagttgga aagcaggtca gaggtcatct ggtacagccc ttccttccct     2520

tttttttttt ttttttttgt gagacaaggt ctctctctgt tgcccaggct ggagtggcgc     2580

aaacacagct cactgcagcc tcaacctact gggctcaagc aatcctccag cctcagcctc     2640

ccaaagtgct gggattacaa gcatgagcca ccccactcag ccctttcctt cctttttaat     2700

tgatgcataa taattgtaag tattcatcat ggtccaacca accctttctt gacccacctt     2760

cctagagaga gggtcctctt gcttcagcgg tcagggcccc agacccatgg tctggctcca     2820

ggtaccacct gcctcatgca ggagttggcg tgcccaggaa gctctgcctc tgggcacagt     2880

gacctcagtg gggtgagggg agctctcccc atagctgggc tgcggcccaa ccccaccccc     2940

tcaggctatg ccagggggtg ttgccagggg cacccgggca tcgccagtct agcccactcc     3000

ttcataaagc cctcgcatcc caggagcgag cagagccaga gcaggatgga gaggagacgc     3060

atcacctccg ctgctcgccg ggcgtacggc caccatgccc aagaagaaga ggaaggtgtc     3120

caatctcctg actgttcacc agaacctccc tgcgctgcca gtagatgcca ctagcgatga     3180

ggtcaggaaa aatctcatgg atatgtttag ggatagacag gcgttttctg aacacacctg     3240

gaaaatgctg cttagcgtgt gccgatcctg ggcagcctgg tgtaagctga acaatcgcaa     3300

atggttcccc gccgagccgg aggacgtgcg cgattacctg ctgtatctcc aggcaagagg     3360

gctggctgtc aagactatcc agcagcactt gggccaactg aatatgctgc atcgacgcag     3420

cgggctcccc cggcctagcg attcaaacgc agtctccctt gttatgagga gaattagaaa     3480

ggaaaacgta gatgcgggtg agagggctaa gcaggctctc gcttttgagc ggactgattt     3540

cgaccaggtc agatccctga tggagaacag cgatcggtgc caggacatca ggaacctcgc     3600

atttctggga attgcatata acacacttct gcgcatagct gagatcgccc ggatcagagt     3660

gaaagacatc agtcgaacgg acggcggccg gatgcttatt catattggac gcacaaagac     3720

attggtcagc accgctggcg ttgaaaaggc cttgtccctg ggcgtaacga agctggtgga     3780

aagatggatc tcagtgtccg gcgtggctga cgaccctaat aattacttgt tctgtcgagt     3840

gagaaaaaac ggagtcgccg cgccctctgc caccagccaa ttgagtacac gggcccttga     3900

agggatcttt gaggcaaccc accgactcat atacggagcc aaggatgaca gtggccagag     3960

gtatctcgcc tggtcaggtc attctgctag ggtgggggcc gcacgagaca tggcgcgggc     4020

aggagtctcc ataccagaga ttatgcaagc tggaggttgg acaaatgtga acatcgttat     4080

gaactatatc cgcaatcttg actctgaaac cggggccatg gtgagactgc tcgaagatgg     4140

tgactaccca tacgatgttc cagattacgc ttaagaattc gatatcaagc ttaataaaag     4200

atctttattt tcattagatc tgtgtgttgg ttttttgtgt ggtaaccacg tgcggaccga     4260

gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct     4320

cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt     4380

gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca     4440

tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc     4500

gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc     4560

ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc     4620

cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc     4680

gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg     4740

gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact     4800

ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt     4860

tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa     4920

atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag     4980

ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc     5040

ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt     5100

tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag     5160

gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg     5220

cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga     5280

caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat     5340

ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca     5400

gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc     5460

gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca     5520

atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg     5580

caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca     5640

gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata     5700

accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag     5760

ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg     5820

gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca     5880

acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta     5940

atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct     6000

ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca     6060

gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag     6120

gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat     6180

tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt     6240

taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa     6300

cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga     6360

gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg     6420

gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc     6480

agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag     6540

aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc     6600

agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg     6660

cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac     6720

accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga     6780

aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt     6840

ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag     6900

cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg     6960

gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt                    7006


<210>  290
<211>  2209
<212>  DNA
<213>  Homo sapiens

<400>  290
cccacctccc tctctgtgct gggactcaca gagggagacc tcaggaggca gtctgtccat       60

cacatgtcca aatgcagagc ataccctggg ctgggcgcag tggcgcacaa ctgtaattcc      120

agcactttgg gaggctgatg tggaaggatc acttgagccc agaagttcta gaccagcctg      180

ggcaacatgg caagacccta tctctacaaa aaaagttaaa aaatcagcca cgtgtggtga      240

cacacacctg tagtcccagc tattcaggag gctgaggtga ggggatcact taaggctggg      300

aggttgaggc tgcagtgagt cgtggttgcg ccactgcact ccagcctggg caacagtgag      360

accctgtctc aaaagacaaa aaaaaaaaaa aaaaaaaaaa gaacatatcc tggtgtggag      420

taggggacgc tgctctgaca gaggctcggg ggcctgagct ggctctgtga gctggggagg      480

aggcagacag ccaggccttg tctgcaagca gacctggcag cattgggctg gccgcccccc      540

agggcctcct cttcatgccc agtgaatgac tcaccttggc acagacacaa tgttcggggt      600

gggcacagtg cctgcttccc gccgcacccc agcccccctc aaatgccttc cgagaagccc      660

attgagcagg gggcttgcat tgcaccccag cctgacagcc tggcatcttg ggataaaagc      720

agcacagccc cctaggggct gcccttgctg tgtggcgcca ccggcggtgg agaacaaggc      780

tctattcagc ctgtgcccag gaaaggggat caggggatgc ccaggcatgg acagtgggtg      840

gcaggggggg agaggagggc tgtctgcttc ccagaagtcc aaggacacaa atgggtgagg      900

ggactgggca gggttctgac cctgtgggac cagagtggag ggcgtagatg gacctgaagt      960

ctccagggac aacagggccc aggtctcagg ctcctagttg ggcccagtgg ctccagcgtt     1020

tccaaaccca tccatcccca gaggttcttc ccatctctcc aggctgatgt gtgggaactc     1080

gaggaaataa atctccagtg ggagacggag gggtggccag ggaaacgggg cgctgcagga     1140

ataaagacga gccagcacag ccagctcatg tgtaacggct ttgtggagct gtcaaggcct     1200

ggtctctggg agagaggcac agggaggcca gacaaggaag gggtgacctg gagggacaga     1260

tccaggggct aaagtcctga taaggcaaga gagtgccggc cccctcttgc cctatcagga     1320

cctccactgc cacatagagg ccatgattga cccttagaca aagggctggt gtccaatccc     1380

agcccccagc cccagaactc cagggaatga atgggcagag agcaggaatg tgggacatct     1440

gtgttcaagg gaaggactcc aggagtctgc tgggaatgag gcctagtagg aaatgaggtg     1500

gcccttgagg gtacagaaca ggttcattct tcgccaaatt cccagcacct tgcaggcact     1560

tacagctgag tgagataatg cctgggttat gaaatcaaaa agttggaaag caggtcagag     1620

gtcatctggt acagcccttc cttccctttt tttttttttt tttttgtgag acaaggtctc     1680

tctctgttgc ccaggctgga gtggcgcaaa cacagctcac tgcagcctca acctactggg     1740

ctcaagcaat cctccagcct cagcctccca aagtgctggg attacaagca tgagccaccc     1800

cactcagccc tttccttcct ttttaattga tgcataataa ttgtaagtat tcatcatggt     1860

ccaaccaacc ctttcttgac ccaccttcct agagagaggg tcctcttgct tcagcggtca     1920

gggccccaga cccatggtct ggctccaggt accacctgcc tcatgcagga gttggcgtgc     1980

ccaggaagct ctgcctctgg gcacagtgac ctcagtgggg tgaggggagc tctccccata     2040

gctgggctgc ggcccaaccc caccccctca ggctatgcca gggggtgttg ccaggggcac     2100

ccgggcatcg ccagtctagc ccactccttc ataaagccct cgcatcccag gagcgagcag     2160

agccagagca ggatggagag gagacgcatc acctccgctg ctcgccggg                 2209


