                         SEQUENCE LISTING

<110>  KWS SAAT SE
 
<120>  Haploidisierung in Sorghum

<130>  KWS0259PCT

<150>  EP17158439.4
<151>  2017-02-28

<160>  45    

<170>  PatentIn version 3.5

<210>  1
<211>  2031
<212>  DNA
<213>  Sorghum bicolor

<400>  1
acgcagtgcc tatatacaca agccaccatc tgtcgttgcc tcctcctcct cagctcatca       60

ctagtcacac gtacacccgc aatcgattga aaatttatcg cgttcgtcca aacaatacca      120

gagctcgacg atcatcttaa ttgttggatc gatctgaata atctttcttc attcgtggcc      180

tcgacgagta agtaggtagc tcatttgatc gatcaaccgc gagtgtgtgc ttgatcgaag      240

gcggcaatgg cgacctacta ctcttcgcgg cgtccatgca acgcctgcag cacgaaggcg      300

atggccggga gcgtggtcgg cgagcccgtc gtgctggggc agagggtgac ggtgctgacg      360

gtggacggcg gcggcatccg tggtctcatc cccggaacca tccttgcctt cctcgaggcc      420

cggctgcagg agctggacgg gccggaggtt aggctcgcgg actacttcga ctacatcgcc      480

gggacgagca ccggcgggct catcaccgcc atgctcaccg cgcccggcaa ggacaggcgg      540

cctctctacg ctgccaagga catcaaccaa ttctacatgg agaattgccc tcgcatcttc      600

cctcaaaagt gagtccgatc atcccggccg gccgccattg ttggcctcgc attattgatc      660

gatcatgcat gcatgcatgc atgtgatgtg taggagcagc aggcttgcgg ccgccatgtc      720

cgcgctgagg aagccaaggt acaacggcaa gtgcctccgt aacctgatca tgagcatgct      780

cggcgagacg agggtgagcg acacgctcac caacgtcatc atccctacct tcgacgtcag      840

gctgctgcag cccatcatct tctccaccta cgacgtacgt acgccgccgg ccgccgtcgt      900

catgaataat caatcagctg tacattattg cacatggctg catgcctact gaacatgttt      960

aatttgcagg ccaagagcat gcctctgaag aacgcgctgc tctccgacgt gtgcatcggc     1020

acgtccgccg cgccgaccta cctcccggcg cactacttcc agaccaagga cgccggcagt     1080

ggcaaggaac gcgagtacaa cctcatcgac ggcggtgtcg ccgccaacaa tccggtaatt     1140

aattaactga aaacgaacga acgcaattcc atatccattg tccagaagat cgatgctaat     1200

tgttgcttag gcacgtcgtt gccgccggcc gattgcattg cacttgcaca tgcgtagacg     1260

atggttgcga tgacgcagat caccaagaag atgcttgcca gcaaggagaa ggccgaggag     1320

ctgtacccag tgaagccgtg gaactgccgc aagttcctgg tgctgtccat cgggacgggg     1380

tcgacgtcgg agcagggcct gtacacggcg cggcagtgct cgcggtgggg catctgccgg     1440

tggatccgga acaacggcat ggcccccatc atcgacatct tcatggcggc gagctcggac     1500

ctggtggaca tccacgtcgc cgcgatgttc cagtcgctcc acagcgacgg cgactacctg     1560

cgcatccagg acaactcgct gcacggcgcc gcggccaccg tggacgcggc gacgccggag     1620

aacatgcgga cgctcgtcgg gatcggggag cggatgctgg cgcagcgggt gtccagggtc     1680

aacgtggaga cagggaggta cgaaccggtg cctggggaag gaagcaacgc tgatgcgctc     1740

gctgggatcg caaggcagct ctcggaggag aggaggacaa ggctcgcgcg ccgcacctcc     1800

gccatcgtca gctccggtgg tgcctctaga cgtacgtgtg cctcaaaggt ctccaatgtc     1860

taagagaagt gaatttgttg cctgattaaa atcttaatta attaattctg tgggctgtgg     1920

ctcaaataaa actatatcat taaaaaaccc gccatgaact agttctagga aaatacgcat     1980

ggttttagaa agaaaaacat aaactaattc taacaaaatg tacacacctc t              2031


<210>  2
<211>  1314
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of Sorghum bicolor Phospholipase

<400>  2
atggcgacct actactcttc gcggcgtcca tgcaacgcct gcagcacgaa ggcgatggcc       60

gggagcgtgg tcggcgagcc cgtcgtgctg gggcagaggg tgacggtgct gacggtggac      120

ggcggcggca tccgtggtct catccccgga accatccttg ccttcctcga ggcccggctg      180

caggagctgg acgggccgga ggttaggctc gcggactact tcgactacat cgccgggacg      240

agcaccggcg ggctcatcac cgccatgctc accgcgcccg gcaaggacag gcggcctctc      300

tacgctgcca aggacatcaa ccaattctac atggagaatt gccctcgcat cttccctcaa      360

aagagcagca ggcttgcggc cgccatgtcc gcgctgagga agccaaggta caacggcaag      420

tgcctccgta acctgatcat gagcatgctc ggcgagacga gggtgagcga cacgctcacc      480

aacgtcatca tccctacctt cgacgtcagg ctgctgcagc ccatcatctt ctccacctac      540

gacgccaaga gcatgcctct gaagaacgcg ctgctctccg acgtgtgcat cggcacgtcc      600

gccgcgccga cctacctccc ggcgcactac ttccagacca aggacgccgg cagtggcaag      660

gaacgcgagt acaacctcat cgacggcggt gtcgccgcca acaatccgac gatggttgcg      720

atgacgcaga tcaccaagaa gatgcttgcc agcaaggaga aggccgagga gctgtaccca      780

gtgaagccgt ggaactgccg caagttcctg gtgctgtcca tcgggacggg gtcgacgtcg      840

gagcagggcc tgtacacggc gcggcagtgc tcgcggtggg gcatctgccg gtggatccgg      900

aacaacggca tggcccccat catcgacatc ttcatggcgg cgagctcgga cctggtggac      960

atccacgtcg ccgcgatgtt ccagtcgctc cacagcgacg gcgactacct gcgcatccag     1020

gacaactcgc tgcacggcgc cgcggccacc gtggacgcgg cgacgccgga gaacatgcgg     1080

acgctcgtcg ggatcgggga gcggatgctg gcgcagcggg tgtccagggt caacgtggag     1140

acagggaggt acgaaccggt gcctggggaa ggaagcaacg ctgatgcgct cgctgggatc     1200

gcaaggcagc tctcggagga gaggaggaca aggctcgcgc gccgcacctc cgccatcgtc     1260

agctccggtg gtgcctctag acgtacgtgt gcctcaaagg tctccaatgt ctaa           1314


<210>  3
<211>  437
<212>  PRT
<213>  Sorghum bicolor

<400>  3

Met Ala Thr Tyr Tyr Ser Ser Arg Arg Pro Cys Asn Ala Cys Ser Thr 
1               5                   10                  15      


Lys Ala Met Ala Gly Ser Val Val Gly Glu Pro Val Val Leu Gly Gln 
            20                  25                  30          


Arg Val Thr Val Leu Thr Val Asp Gly Gly Gly Ile Arg Gly Leu Ile 
        35                  40                  45              


Pro Gly Thr Ile Leu Ala Phe Leu Glu Ala Arg Leu Gln Glu Leu Asp 
    50                  55                  60                  


Gly Pro Glu Val Arg Leu Ala Asp Tyr Phe Asp Tyr Ile Ala Gly Thr 
65                  70                  75                  80  


Ser Thr Gly Gly Leu Ile Thr Ala Met Leu Thr Ala Pro Gly Lys Asp 
                85                  90                  95      


Arg Arg Pro Leu Tyr Ala Ala Lys Asp Ile Asn Gln Phe Tyr Met Glu 
            100                 105                 110         


Asn Cys Pro Arg Ile Phe Pro Gln Lys Ser Ser Arg Leu Ala Ala Ala 
        115                 120                 125             


Met Ser Ala Leu Arg Lys Pro Arg Tyr Asn Gly Lys Cys Leu Arg Asn 
    130                 135                 140                 


Leu Ile Met Ser Met Leu Gly Glu Thr Arg Val Ser Asp Thr Leu Thr 
145                 150                 155                 160 


Asn Val Ile Ile Pro Thr Phe Asp Val Arg Leu Leu Gln Pro Ile Ile 
                165                 170                 175     


Phe Ser Thr Tyr Asp Ala Lys Ser Met Pro Leu Lys Asn Ala Leu Leu 
            180                 185                 190         


Ser Asp Val Cys Ile Gly Thr Ser Ala Ala Pro Thr Tyr Leu Pro Ala 
        195                 200                 205             


His Tyr Phe Gln Thr Lys Asp Ala Gly Ser Gly Lys Glu Arg Glu Tyr 
    210                 215                 220                 


Asn Leu Ile Asp Gly Gly Val Ala Ala Asn Asn Pro Thr Met Val Ala 
225                 230                 235                 240 


Met Thr Gln Ile Thr Lys Lys Met Leu Ala Ser Lys Glu Lys Ala Glu 
                245                 250                 255     


Glu Leu Tyr Pro Val Lys Pro Trp Asn Cys Arg Lys Phe Leu Val Leu 
            260                 265                 270         


Ser Ile Gly Thr Gly Ser Thr Ser Glu Gln Gly Leu Tyr Thr Ala Arg 
        275                 280                 285             


Gln Cys Ser Arg Trp Gly Ile Cys Arg Trp Ile Arg Asn Asn Gly Met 
    290                 295                 300                 


Ala Pro Ile Ile Asp Ile Phe Met Ala Ala Ser Ser Asp Leu Val Asp 
305                 310                 315                 320 


Ile His Val Ala Ala Met Phe Gln Ser Leu His Ser Asp Gly Asp Tyr 
                325                 330                 335     


Leu Arg Ile Gln Asp Asn Ser Leu His Gly Ala Ala Ala Thr Val Asp 
            340                 345                 350         


Ala Ala Thr Pro Glu Asn Met Arg Thr Leu Val Gly Ile Gly Glu Arg 
        355                 360                 365             


Met Leu Ala Gln Arg Val Ser Arg Val Asn Val Glu Thr Gly Arg Tyr 
    370                 375                 380                 


Glu Pro Val Pro Gly Glu Gly Ser Asn Ala Asp Ala Leu Ala Gly Ile 
385                 390                 395                 400 


Ala Arg Gln Leu Ser Glu Glu Arg Arg Thr Arg Leu Ala Arg Arg Thr 
                405                 410                 415     


Ser Ala Ile Val Ser Ser Gly Gly Ala Ser Arg Arg Thr Cys Ala Ser 
            420                 425                 430         


Lys Val Ser Asn Val 
        435         


<210>  4
<211>  2031
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic DNA of mutated Sorghum bicolor phospholipase R59Q

<400>  4
acgcagtgcc tatatacaca agccaccatc tgtcgttgcc tcctcctcct cagctcatca       60

ctagtcacac gtacacccgc aatcgattga aaatttatcg cgttcgtcca aacaatacca      120

gagctcgacg atcatcttaa ttgttggatc gatctgaata atctttcttc attcgtggcc      180

tcgacgagta agtaggtagc tcatttgatc gatcaaccgc gagtgtgtgc ttgatcgaag      240

gcggcaatgg cgacctacta ctcttcgcgg cgtccatgca acgcctgcag cacgaaggcg      300

atggccggga gcgtggtcgg cgagcccgtc gtgctggggc agagggtgac ggtgctgacg      360

gtggacggcg gcggcatccg tggtctcatc cccggaacca tccttgcctt cctcgaggcc      420

cagctgcagg agctggacgg gccggaggtt aggctcgcgg actacttcga ctacatcgcc      480

gggacgagca ccggcgggct catcaccgcc atgctcaccg cgcccggcaa ggacaggcgg      540

cctctctacg ctgccaagga catcaaccaa ttctacatgg agaattgccc tcgcatcttc      600

cctcaaaagt gagtccgatc atcccggccg gccgccattg ttggcctcgc attattgatc      660

gatcatgcat gcatgcatgc atgtgatgtg taggagcagc aggcttgcgg ccgccatgtc      720

cgcgctgagg aagccaaggt acaacggcaa gtgcctccgt aacctgatca tgagcatgct      780

cggcgagacg agggtgagcg acacgctcac caacgtcatc atccctacct tcgacgtcag      840

gctgctgcag cccatcatct tctccaccta cgacgtacgt acgccgccgg ccgccgtcgt      900

catgaataat caatcagctg tacattattg cacatggctg catgcctact gaacatgttt      960

aatttgcagg ccaagagcat gcctctgaag aacgcgctgc tctccgacgt gtgcatcggc     1020

acgtccgccg cgccgaccta cctcccggcg cactacttcc agaccaagga cgccggcagt     1080

ggcaaggaac gcgagtacaa cctcatcgac ggcggtgtcg ccgccaacaa tccggtaatt     1140

aattaactga aaacgaacga acgcaattcc atatccattg tccagaagat cgatgctaat     1200

tgttgcttag gcacgtcgtt gccgccggcc gattgcattg cacttgcaca tgcgtagacg     1260

atggttgcga tgacgcagat caccaagaag atgcttgcca gcaaggagaa ggccgaggag     1320

ctgtacccag tgaagccgtg gaactgccgc aagttcctgg tgctgtccat cgggacgggg     1380

tcgacgtcgg agcagggcct gtacacggcg cggcagtgct cgcggtgggg catctgccgg     1440

tggatccgga acaacggcat ggcccccatc atcgacatct tcatggcggc gagctcggac     1500

ctggtggaca tccacgtcgc cgcgatgttc cagtcgctcc acagcgacgg cgactacctg     1560

cgcatccagg acaactcgct gcacggcgcc gcggccaccg tggacgcggc gacgccggag     1620

aacatgcgga cgctcgtcgg gatcggggag cggatgctgg cgcagcgggt gtccagggtc     1680

aacgtggaga cagggaggta cgaaccggtg cctggggaag gaagcaacgc tgatgcgctc     1740

gctgggatcg caaggcagct ctcggaggag aggaggacaa ggctcgcgcg ccgcacctcc     1800

gccatcgtca gctccggtgg tgcctctaga cgtacgtgtg cctcaaaggt ctccaatgtc     1860

taagagaagt gaatttgttg cctgattaaa atcttaatta attaattctg tgggctgtgg     1920

ctcaaataaa actatatcat taaaaaaccc gccatgaact agttctagga aaatacgcat     1980

ggttttagaa agaaaaacat aaactaattc taacaaaatg tacacacctc t              2031


<210>  5
<211>  1314
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of Sorghum bicolor phospholipase mutant R59Q

<400>  5
atggcgacct actactcttc gcggcgtcca tgcaacgcct gcagcacgaa ggcgatggcc       60

gggagcgtgg tcggcgagcc cgtcgtgctg gggcagaggg tgacggtgct gacggtggac      120

ggcggcggca tccgtggtct catccccgga accatccttg ccttcctcga ggcccagctg      180

caggagctgg acgggccgga ggttaggctc gcggactact tcgactacat cgccgggacg      240

agcaccggcg ggctcatcac cgccatgctc accgcgcccg gcaaggacag gcggcctctc      300

tacgctgcca aggacatcaa ccaattctac atggagaatt gccctcgcat cttccctcaa      360

aagagcagca ggcttgcggc cgccatgtcc gcgctgagga agccaaggta caacggcaag      420

tgcctccgta acctgatcat gagcatgctc ggcgagacga gggtgagcga cacgctcacc      480

aacgtcatca tccctacctt cgacgtcagg ctgctgcagc ccatcatctt ctccacctac      540

gacgccaaga gcatgcctct gaagaacgcg ctgctctccg acgtgtgcat cggcacgtcc      600

gccgcgccga cctacctccc ggcgcactac ttccagacca aggacgccgg cagtggcaag      660

gaacgcgagt acaacctcat cgacggcggt gtcgccgcca acaatccgac gatggttgcg      720

atgacgcaga tcaccaagaa gatgcttgcc agcaaggaga aggccgagga gctgtaccca      780

gtgaagccgt ggaactgccg caagttcctg gtgctgtcca tcgggacggg gtcgacgtcg      840

gagcagggcc tgtacacggc gcggcagtgc tcgcggtggg gcatctgccg gtggatccgg      900

aacaacggca tggcccccat catcgacatc ttcatggcgg cgagctcgga cctggtggac      960

atccacgtcg ccgcgatgtt ccagtcgctc cacagcgacg gcgactacct gcgcatccag     1020

gacaactcgc tgcacggcgc cgcggccacc gtggacgcgg cgacgccgga gaacatgcgg     1080

acgctcgtcg ggatcgggga gcggatgctg gcgcagcggg tgtccagggt caacgtggag     1140

acagggaggt acgaaccggt gcctggggaa ggaagcaacg ctgatgcgct cgctgggatc     1200

gcaaggcagc tctcggagga gaggaggaca aggctcgcgc gccgcacctc cgccatcgtc     1260

agctccggtg gtgcctctag acgtacgtgt gcctcaaagg tctccaatgt ctaa           1314


<210>  6
<211>  437
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of mutated Sorghum bicolor phospholipase R59Q

<400>  6

Met Ala Thr Tyr Tyr Ser Ser Arg Arg Pro Cys Asn Ala Cys Ser Thr 
1               5                   10                  15      


Lys Ala Met Ala Gly Ser Val Val Gly Glu Pro Val Val Leu Gly Gln 
            20                  25                  30          


Arg Val Thr Val Leu Thr Val Asp Gly Gly Gly Ile Arg Gly Leu Ile 
        35                  40                  45              


Pro Gly Thr Ile Leu Ala Phe Leu Glu Ala Gln Leu Gln Glu Leu Asp 
    50                  55                  60                  


Gly Pro Glu Val Arg Leu Ala Asp Tyr Phe Asp Tyr Ile Ala Gly Thr 
65                  70                  75                  80  


Ser Thr Gly Gly Leu Ile Thr Ala Met Leu Thr Ala Pro Gly Lys Asp 
                85                  90                  95      


Arg Arg Pro Leu Tyr Ala Ala Lys Asp Ile Asn Gln Phe Tyr Met Glu 
            100                 105                 110         


Asn Cys Pro Arg Ile Phe Pro Gln Lys Ser Ser Arg Leu Ala Ala Ala 
        115                 120                 125             


Met Ser Ala Leu Arg Lys Pro Arg Tyr Asn Gly Lys Cys Leu Arg Asn 
    130                 135                 140                 


Leu Ile Met Ser Met Leu Gly Glu Thr Arg Val Ser Asp Thr Leu Thr 
145                 150                 155                 160 


Asn Val Ile Ile Pro Thr Phe Asp Val Arg Leu Leu Gln Pro Ile Ile 
                165                 170                 175     


Phe Ser Thr Tyr Asp Ala Lys Ser Met Pro Leu Lys Asn Ala Leu Leu 
            180                 185                 190         


Ser Asp Val Cys Ile Gly Thr Ser Ala Ala Pro Thr Tyr Leu Pro Ala 
        195                 200                 205             


His Tyr Phe Gln Thr Lys Asp Ala Gly Ser Gly Lys Glu Arg Glu Tyr 
    210                 215                 220                 


Asn Leu Ile Asp Gly Gly Val Ala Ala Asn Asn Pro Thr Met Val Ala 
225                 230                 235                 240 


Met Thr Gln Ile Thr Lys Lys Met Leu Ala Ser Lys Glu Lys Ala Glu 
                245                 250                 255     


Glu Leu Tyr Pro Val Lys Pro Trp Asn Cys Arg Lys Phe Leu Val Leu 
            260                 265                 270         


Ser Ile Gly Thr Gly Ser Thr Ser Glu Gln Gly Leu Tyr Thr Ala Arg 
        275                 280                 285             


Gln Cys Ser Arg Trp Gly Ile Cys Arg Trp Ile Arg Asn Asn Gly Met 
    290                 295                 300                 


Ala Pro Ile Ile Asp Ile Phe Met Ala Ala Ser Ser Asp Leu Val Asp 
305                 310                 315                 320 


Ile His Val Ala Ala Met Phe Gln Ser Leu His Ser Asp Gly Asp Tyr 
                325                 330                 335     


Leu Arg Ile Gln Asp Asn Ser Leu His Gly Ala Ala Ala Thr Val Asp 
            340                 345                 350         


Ala Ala Thr Pro Glu Asn Met Arg Thr Leu Val Gly Ile Gly Glu Arg 
        355                 360                 365             


Met Leu Ala Gln Arg Val Ser Arg Val Asn Val Glu Thr Gly Arg Tyr 
    370                 375                 380                 


Glu Pro Val Pro Gly Glu Gly Ser Asn Ala Asp Ala Leu Ala Gly Ile 
385                 390                 395                 400 


Ala Arg Gln Leu Ser Glu Glu Arg Arg Thr Arg Leu Ala Arg Arg Thr 
                405                 410                 415     


Ser Ala Ile Val Ser Ser Gly Gly Ala Ser Arg Arg Thr Cys Ala Ser 
            420                 425                 430         


Lys Val Ser Asn Val 
        435         


<210>  7
<211>  2031
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic DNA of mutated Sorghum bicolor phospholipase V162I

<400>  7
acgcagtgcc tatatacaca agccaccatc tgtcgttgcc tcctcctcct cagctcatca       60

ctagtcacac gtacacccgc aatcgattga aaatttatcg cgttcgtcca aacaatacca      120

gagctcgacg atcatcttaa ttgttggatc gatctgaata atctttcttc attcgtggcc      180

tcgacgagta agtaggtagc tcatttgatc gatcaaccgc gagtgtgtgc ttgatcgaag      240

gcggcaatgg cgacctacta ctcttcgcgg cgtccatgca acgcctgcag cacgaaggcg      300

atggccggga gcgtggtcgg cgagcccgtc gtgctggggc agagggtgac ggtgctgacg      360

gtggacggcg gcggcatccg tggtctcatc cccggaacca tccttgcctt cctcgaggcc      420

cggctgcagg agctggacgg gccggaggtt aggctcgcgg actacttcga ctacatcgcc      480

gggacgagca ccggcgggct catcaccgcc atgctcaccg cgcccggcaa ggacaggcgg      540

cctctctacg ctgccaagga catcaaccaa ttctacatgg agaattgccc tcgcatcttc      600

cctcaaaagt gagtccgatc atcccggccg gccgccattg ttggcctcgc attattgatc      660

gatcatgcat gcatgcatgc atgtgatgtg taggagcagc aggcttgcgg ccgccatgtc      720

cgcgctgagg aagccaaggt acaacggcaa gtgcctccgt aacctgatca tgagcatgct      780

cggcgagacg agggtgagcg acacgctcac caacatcatc atccctacct tcgacgtcag      840

gctgctgcag cccatcatct tctccaccta cgacgtacgt acgccgccgg ccgccgtcgt      900

catgaataat caatcagctg tacattattg cacatggctg catgcctact gaacatgttt      960

aatttgcagg ccaagagcat gcctctgaag aacgcgctgc tctccgacgt gtgcatcggc     1020

acgtccgccg cgccgaccta cctcccggcg cactacttcc agaccaagga cgccggcagt     1080

ggcaaggaac gcgagtacaa cctcatcgac ggcggtgtcg ccgccaacaa tccggtaatt     1140

aattaactga aaacgaacga acgcaattcc atatccattg tccagaagat cgatgctaat     1200

tgttgcttag gcacgtcgtt gccgccggcc gattgcattg cacttgcaca tgcgtagacg     1260

atggttgcga tgacgcagat caccaagaag atgcttgcca gcaaggagaa ggccgaggag     1320

ctgtacccag tgaagccgtg gaactgccgc aagttcctgg tgctgtccat cgggacgggg     1380

tcgacgtcgg agcagggcct gtacacggcg cggcagtgct cgcggtgggg catctgccgg     1440

tggatccgga acaacggcat ggcccccatc atcgacatct tcatggcggc gagctcggac     1500

ctggtggaca tccacgtcgc cgcgatgttc cagtcgctcc acagcgacgg cgactacctg     1560

cgcatccagg acaactcgct gcacggcgcc gcggccaccg tggacgcggc gacgccggag     1620

aacatgcgga cgctcgtcgg gatcggggag cggatgctgg cgcagcgggt gtccagggtc     1680

aacgtggaga cagggaggta cgaaccggtg cctggggaag gaagcaacgc tgatgcgctc     1740

gctgggatcg caaggcagct ctcggaggag aggaggacaa ggctcgcgcg ccgcacctcc     1800

gccatcgtca gctccggtgg tgcctctaga cgtacgtgtg cctcaaaggt ctccaatgtc     1860

taagagaagt gaatttgttg cctgattaaa atcttaatta attaattctg tgggctgtgg     1920

ctcaaataaa actatatcat taaaaaaccc gccatgaact agttctagga aaatacgcat     1980

ggttttagaa agaaaaacat aaactaattc taacaaaatg tacacacctc t              2031


<210>  8
<211>  1314
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA Sorghum bicolor Phospholipase mutant V162I

<400>  8
atggcgacct actactcttc gcggcgtcca tgcaacgcct gcagcacgaa ggcgatggcc       60

gggagcgtgg tcggcgagcc cgtcgtgctg gggcagaggg tgacggtgct gacggtggac      120

ggcggcggca tccgtggtct catccccgga accatccttg ccttcctcga ggcccggctg      180

caggagctgg acgggccgga ggttaggctc gcggactact tcgactacat cgccgggacg      240

agcaccggcg ggctcatcac cgccatgctc accgcgcccg gcaaggacag gcggcctctc      300

tacgctgcca aggacatcaa ccaattctac atggagaatt gccctcgcat cttccctcaa      360

aagagcagca ggcttgcggc cgccatgtcc gcgctgagga agccaaggta caacggcaag      420

tgcctccgta acctgatcat gagcatgctc ggcgagacga gggtgagcga cacgctcacc      480

aacatcatca tccctacctt cgacgtcagg ctgctgcagc ccatcatctt ctccacctac      540

gacgccaaga gcatgcctct gaagaacgcg ctgctctccg acgtgtgcat cggcacgtcc      600

gccgcgccga cctacctccc ggcgcactac ttccagacca aggacgccgg cagtggcaag      660

gaacgcgagt acaacctcat cgacggcggt gtcgccgcca acaatccgac gatggttgcg      720

atgacgcaga tcaccaagaa gatgcttgcc agcaaggaga aggccgagga gctgtaccca      780

gtgaagccgt ggaactgccg caagttcctg gtgctgtcca tcgggacggg gtcgacgtcg      840

gagcagggcc tgtacacggc gcggcagtgc tcgcggtggg gcatctgccg gtggatccgg      900

aacaacggca tggcccccat catcgacatc ttcatggcgg cgagctcgga cctggtggac      960

atccacgtcg ccgcgatgtt ccagtcgctc cacagcgacg gcgactacct gcgcatccag     1020

gacaactcgc tgcacggcgc cgcggccacc gtggacgcgg cgacgccgga gaacatgcgg     1080

acgctcgtcg ggatcgggga gcggatgctg gcgcagcggg tgtccagggt caacgtggag     1140

acagggaggt acgaaccggt gcctggggaa ggaagcaacg ctgatgcgct cgctgggatc     1200

gcaaggcagc tctcggagga gaggaggaca aggctcgcgc gccgcacctc cgccatcgtc     1260

agctccggtg gtgcctctag acgtacgtgt gcctcaaagg tctccaatgt ctaa           1314


<210>  9
<211>  437
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of mutated Sorghum bicolor phospholipase 
       V162I

<400>  9

Met Ala Thr Tyr Tyr Ser Ser Arg Arg Pro Cys Asn Ala Cys Ser Thr 
1               5                   10                  15      


Lys Ala Met Ala Gly Ser Val Val Gly Glu Pro Val Val Leu Gly Gln 
            20                  25                  30          


Arg Val Thr Val Leu Thr Val Asp Gly Gly Gly Ile Arg Gly Leu Ile 
        35                  40                  45              


Pro Gly Thr Ile Leu Ala Phe Leu Glu Ala Arg Leu Gln Glu Leu Asp 
    50                  55                  60                  


Gly Pro Glu Val Arg Leu Ala Asp Tyr Phe Asp Tyr Ile Ala Gly Thr 
65                  70                  75                  80  


Ser Thr Gly Gly Leu Ile Thr Ala Met Leu Thr Ala Pro Gly Lys Asp 
                85                  90                  95      


Arg Arg Pro Leu Tyr Ala Ala Lys Asp Ile Asn Gln Phe Tyr Met Glu 
            100                 105                 110         


Asn Cys Pro Arg Ile Phe Pro Gln Lys Ser Ser Arg Leu Ala Ala Ala 
        115                 120                 125             


Met Ser Ala Leu Arg Lys Pro Arg Tyr Asn Gly Lys Cys Leu Arg Asn 
    130                 135                 140                 


Leu Ile Met Ser Met Leu Gly Glu Thr Arg Val Ser Asp Thr Leu Thr 
145                 150                 155                 160 


Asn Ile Ile Ile Pro Thr Phe Asp Val Arg Leu Leu Gln Pro Ile Ile 
                165                 170                 175     


Phe Ser Thr Tyr Asp Ala Lys Ser Met Pro Leu Lys Asn Ala Leu Leu 
            180                 185                 190         


Ser Asp Val Cys Ile Gly Thr Ser Ala Ala Pro Thr Tyr Leu Pro Ala 
        195                 200                 205             


His Tyr Phe Gln Thr Lys Asp Ala Gly Ser Gly Lys Glu Arg Glu Tyr 
    210                 215                 220                 


Asn Leu Ile Asp Gly Gly Val Ala Ala Asn Asn Pro Thr Met Val Ala 
225                 230                 235                 240 


Met Thr Gln Ile Thr Lys Lys Met Leu Ala Ser Lys Glu Lys Ala Glu 
                245                 250                 255     


Glu Leu Tyr Pro Val Lys Pro Trp Asn Cys Arg Lys Phe Leu Val Leu 
            260                 265                 270         


Ser Ile Gly Thr Gly Ser Thr Ser Glu Gln Gly Leu Tyr Thr Ala Arg 
        275                 280                 285             


Gln Cys Ser Arg Trp Gly Ile Cys Arg Trp Ile Arg Asn Asn Gly Met 
    290                 295                 300                 


Ala Pro Ile Ile Asp Ile Phe Met Ala Ala Ser Ser Asp Leu Val Asp 
305                 310                 315                 320 


Ile His Val Ala Ala Met Phe Gln Ser Leu His Ser Asp Gly Asp Tyr 
                325                 330                 335     


Leu Arg Ile Gln Asp Asn Ser Leu His Gly Ala Ala Ala Thr Val Asp 
            340                 345                 350         


Ala Ala Thr Pro Glu Asn Met Arg Thr Leu Val Gly Ile Gly Glu Arg 
        355                 360                 365             


Met Leu Ala Gln Arg Val Ser Arg Val Asn Val Glu Thr Gly Arg Tyr 
    370                 375                 380                 


Glu Pro Val Pro Gly Glu Gly Ser Asn Ala Asp Ala Leu Ala Gly Ile 
385                 390                 395                 400 


Ala Arg Gln Leu Ser Glu Glu Arg Arg Thr Arg Leu Ala Arg Arg Thr 
                405                 410                 415     


Ser Ala Ile Val Ser Ser Gly Gly Ala Ser Arg Arg Thr Cys Ala Ser 
            420                 425                 430         


Lys Val Ser Asn Val 
        435         


<210>  10
<211>  2031
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic DNA of mutated Sorghum bicolor phospholipase S291L

<400>  10
acgcagtgcc tatatacaca agccaccatc tgtcgttgcc tcctcctcct cagctcatca       60

ctagtcacac gtacacccgc aatcgattga aaatttatcg cgttcgtcca aacaatacca      120

gagctcgacg atcatcttaa ttgttggatc gatctgaata atctttcttc attcgtggcc      180

tcgacgagta agtaggtagc tcatttgatc gatcaaccgc gagtgtgtgc ttgatcgaag      240

gcggcaatgg cgacctacta ctcttcgcgg cgtccatgca acgcctgcag cacgaaggcg      300

atggccggga gcgtggtcgg cgagcccgtc gtgctggggc agagggtgac ggtgctgacg      360

gtggacggcg gcggcatccg tggtctcatc cccggaacca tccttgcctt cctcgaggcc      420

cggctgcagg agctggacgg gccggaggtt aggctcgcgg actacttcga ctacatcgcc      480

gggacgagca ccggcgggct catcaccgcc atgctcaccg cgcccggcaa ggacaggcgg      540

cctctctacg ctgccaagga catcaaccaa ttctacatgg agaattgccc tcgcatcttc      600

cctcaaaagt gagtccgatc atcccggccg gccgccattg ttggcctcgc attattgatc      660

gatcatgcat gcatgcatgc atgtgatgtg taggagcagc aggcttgcgg ccgccatgtc      720

cgcgctgagg aagccaaggt acaacggcaa gtgcctccgt aacctgatca tgagcatgct      780

cggcgagacg agggtgagcg acacgctcac caacgtcatc atccctacct tcgacgtcag      840

gctgctgcag cccatcatct tctccaccta cgacgtacgt acgccgccgg ccgccgtcgt      900

catgaataat caatcagctg tacattattg cacatggctg catgcctact gaacatgttt      960

aatttgcagg ccaagagcat gcctctgaag aacgcgctgc tctccgacgt gtgcatcggc     1020

acgtccgccg cgccgaccta cctcccggcg cactacttcc agaccaagga cgccggcagt     1080

ggcaaggaac gcgagtacaa cctcatcgac ggcggtgtcg ccgccaacaa tccggtaatt     1140

aattaactga aaacgaacga acgcaattcc atatccattg tccagaagat cgatgctaat     1200

tgttgcttag gcacgtcgtt gccgccggcc gattgcattg cacttgcaca tgcgtagacg     1260

atggttgcga tgacgcagat caccaagaag atgcttgcca gcaaggagaa ggccgaggag     1320

ctgtacccag tgaagccgtg gaactgccgc aagttcctgg tgctgtccat cgggacgggg     1380

tcgacgtcgg agcagggcct gtacacggcg cggcagtgct tgcggtgggg catctgccgg     1440

tggatccgga acaacggcat ggcccccatc atcgacatct tcatggcggc gagctcggac     1500

ctggtggaca tccacgtcgc cgcgatgttc cagtcgctcc acagcgacgg cgactacctg     1560

cgcatccagg acaactcgct gcacggcgcc gcggccaccg tggacgcggc gacgccggag     1620

aacatgcgga cgctcgtcgg gatcggggag cggatgctgg cgcagcgggt gtccagggtc     1680

aacgtggaga cagggaggta cgaaccggtg cctggggaag gaagcaacgc tgatgcgctc     1740

gctgggatcg caaggcagct ctcggaggag aggaggacaa ggctcgcgcg ccgcacctcc     1800

gccatcgtca gctccggtgg tgcctctaga cgtacgtgtg cctcaaaggt ctccaatgtc     1860

taagagaagt gaatttgttg cctgattaaa atcttaatta attaattctg tgggctgtgg     1920

ctcaaataaa actatatcat taaaaaaccc gccatgaact agttctagga aaatacgcat     1980

ggttttagaa agaaaaacat aaactaattc taacaaaatg tacacacctc t              2031


<210>  11
<211>  1314
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of Sorghum bicolor phospholipase mutant S291L

<400>  11
atggcgacct actactcttc gcggcgtcca tgcaacgcct gcagcacgaa ggcgatggcc       60

gggagcgtgg tcggcgagcc cgtcgtgctg gggcagaggg tgacggtgct gacggtggac      120

ggcggcggca tccgtggtct catccccgga accatccttg ccttcctcga ggcccggctg      180

caggagctgg acgggccgga ggttaggctc gcggactact tcgactacat cgccgggacg      240

agcaccggcg ggctcatcac cgccatgctc accgcgcccg gcaaggacag gcggcctctc      300

tacgctgcca aggacatcaa ccaattctac atggagaatt gccctcgcat cttccctcaa      360

aagagcagca ggcttgcggc cgccatgtcc gcgctgagga agccaaggta caacggcaag      420

tgcctccgta acctgatcat gagcatgctc ggcgagacga gggtgagcga cacgctcacc      480

aacgtcatca tccctacctt cgacgtcagg ctgctgcagc ccatcatctt ctccacctac      540

gacgccaaga gcatgcctct gaagaacgcg ctgctctccg acgtgtgcat cggcacgtcc      600

gccgcgccga cctacctccc ggcgcactac ttccagacca aggacgccgg cagtggcaag      660

gaacgcgagt acaacctcat cgacggcggt gtcgccgcca acaatccgac gatggttgcg      720

atgacgcaga tcaccaagaa gatgcttgcc agcaaggaga aggccgagga gctgtaccca      780

gtgaagccgt ggaactgccg caagttcctg gtgctgtcca tcgggacggg gtcgacgtcg      840

gagcagggcc tgtacacggc gcggcagtgc ttgcggtggg gcatctgccg gtggatccgg      900

aacaacggca tggcccccat catcgacatc ttcatggcgg cgagctcgga cctggtggac      960

atccacgtcg ccgcgatgtt ccagtcgctc cacagcgacg gcgactacct gcgcatccag     1020

gacaactcgc tgcacggcgc cgcggccacc gtggacgcgg cgacgccgga gaacatgcgg     1080

acgctcgtcg ggatcgggga gcggatgctg gcgcagcggg tgtccagggt caacgtggag     1140

acagggaggt acgaaccggt gcctggggaa ggaagcaacg ctgatgcgct cgctgggatc     1200

gcaaggcagc tctcggagga gaggaggaca aggctcgcgc gccgcacctc cgccatcgtc     1260

agctccggtg gtgcctctag acgtacgtgt gcctcaaagg tctccaatgt ctaa           1314


<210>  12
<211>  437
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of mutated Sorghum bicolor phospholipase 
       S291L

<400>  12

Met Ala Thr Tyr Tyr Ser Ser Arg Arg Pro Cys Asn Ala Cys Ser Thr 
1               5                   10                  15      


Lys Ala Met Ala Gly Ser Val Val Gly Glu Pro Val Val Leu Gly Gln 
            20                  25                  30          


Arg Val Thr Val Leu Thr Val Asp Gly Gly Gly Ile Arg Gly Leu Ile 
        35                  40                  45              


Pro Gly Thr Ile Leu Ala Phe Leu Glu Ala Arg Leu Gln Glu Leu Asp 
    50                  55                  60                  


Gly Pro Glu Val Arg Leu Ala Asp Tyr Phe Asp Tyr Ile Ala Gly Thr 
65                  70                  75                  80  


Ser Thr Gly Gly Leu Ile Thr Ala Met Leu Thr Ala Pro Gly Lys Asp 
                85                  90                  95      


Arg Arg Pro Leu Tyr Ala Ala Lys Asp Ile Asn Gln Phe Tyr Met Glu 
            100                 105                 110         


Asn Cys Pro Arg Ile Phe Pro Gln Lys Ser Ser Arg Leu Ala Ala Ala 
        115                 120                 125             


Met Ser Ala Leu Arg Lys Pro Arg Tyr Asn Gly Lys Cys Leu Arg Asn 
    130                 135                 140                 


Leu Ile Met Ser Met Leu Gly Glu Thr Arg Val Ser Asp Thr Leu Thr 
145                 150                 155                 160 


Asn Val Ile Ile Pro Thr Phe Asp Val Arg Leu Leu Gln Pro Ile Ile 
                165                 170                 175     


Phe Ser Thr Tyr Asp Ala Lys Ser Met Pro Leu Lys Asn Ala Leu Leu 
            180                 185                 190         


Ser Asp Val Cys Ile Gly Thr Ser Ala Ala Pro Thr Tyr Leu Pro Ala 
        195                 200                 205             


His Tyr Phe Gln Thr Lys Asp Ala Gly Ser Gly Lys Glu Arg Glu Tyr 
    210                 215                 220                 


Asn Leu Ile Asp Gly Gly Val Ala Ala Asn Asn Pro Thr Met Val Ala 
225                 230                 235                 240 


Met Thr Gln Ile Thr Lys Lys Met Leu Ala Ser Lys Glu Lys Ala Glu 
                245                 250                 255     


Glu Leu Tyr Pro Val Lys Pro Trp Asn Cys Arg Lys Phe Leu Val Leu 
            260                 265                 270         


Ser Ile Gly Thr Gly Ser Thr Ser Glu Gln Gly Leu Tyr Thr Ala Arg 
        275                 280                 285             


Gln Cys Leu Arg Trp Gly Ile Cys Arg Trp Ile Arg Asn Asn Gly Met 
    290                 295                 300                 


Ala Pro Ile Ile Asp Ile Phe Met Ala Ala Ser Ser Asp Leu Val Asp 
305                 310                 315                 320 


Ile His Val Ala Ala Met Phe Gln Ser Leu His Ser Asp Gly Asp Tyr 
                325                 330                 335     


Leu Arg Ile Gln Asp Asn Ser Leu His Gly Ala Ala Ala Thr Val Asp 
            340                 345                 350         


Ala Ala Thr Pro Glu Asn Met Arg Thr Leu Val Gly Ile Gly Glu Arg 
        355                 360                 365             


Met Leu Ala Gln Arg Val Ser Arg Val Asn Val Glu Thr Gly Arg Tyr 
    370                 375                 380                 


Glu Pro Val Pro Gly Glu Gly Ser Asn Ala Asp Ala Leu Ala Gly Ile 
385                 390                 395                 400 


Ala Arg Gln Leu Ser Glu Glu Arg Arg Thr Arg Leu Ala Arg Arg Thr 
                405                 410                 415     


Ser Ala Ile Val Ser Ser Gly Gly Ala Ser Arg Arg Thr Cys Ala Ser 
            420                 425                 430         


Lys Val Ser Asn Val 
        435         


<210>  13
<211>  2031
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic DNA of mutated Sorghum bicolor phospholipase Q372stop

<400>  13
acgcagtgcc tatatacaca agccaccatc tgtcgttgcc tcctcctcct cagctcatca       60

ctagtcacac gtacacccgc aatcgattga aaatttatcg cgttcgtcca aacaatacca      120

gagctcgacg atcatcttaa ttgttggatc gatctgaata atctttcttc attcgtggcc      180

tcgacgagta agtaggtagc tcatttgatc gatcaaccgc gagtgtgtgc ttgatcgaag      240

gcggcaatgg cgacctacta ctcttcgcgg cgtccatgca acgcctgcag cacgaaggcg      300

atggccggga gcgtggtcgg cgagcccgtc gtgctggggc agagggtgac ggtgctgacg      360

gtggacggcg gcggcatccg tggtctcatc cccggaacca tccttgcctt cctcgaggcc      420

cggctgcagg agctggacgg gccggaggtt aggctcgcgg actacttcga ctacatcgcc      480

gggacgagca ccggcgggct catcaccgcc atgctcaccg cgcccggcaa ggacaggcgg      540

cctctctacg ctgccaagga catcaaccaa ttctacatgg agaattgccc tcgcatcttc      600

cctcaaaagt gagtccgatc atcccggccg gccgccattg ttggcctcgc attattgatc      660

gatcatgcat gcatgcatgc atgtgatgtg taggagcagc aggcttgcgg ccgccatgtc      720

cgcgctgagg aagccaaggt acaacggcaa gtgcctccgt aacctgatca tgagcatgct      780

cggcgagacg agggtgagcg acacgctcac caacgtcatc atccctacct tcgacgtcag      840

gctgctgcag cccatcatct tctccaccta cgacgtacgt acgccgccgg ccgccgtcgt      900

catgaataat caatcagctg tacattattg cacatggctg catgcctact gaacatgttt      960

aatttgcagg ccaagagcat gcctctgaag aacgcgctgc tctccgacgt gtgcatcggc     1020

acgtccgccg cgccgaccta cctcccggcg cactacttcc agaccaagga cgccggcagt     1080

ggcaaggaac gcgagtacaa cctcatcgac ggcggtgtcg ccgccaacaa tccggtaatt     1140

aattaactga aaacgaacga acgcaattcc atatccattg tccagaagat cgatgctaat     1200

tgttgcttag gcacgtcgtt gccgccggcc gattgcattg cacttgcaca tgcgtagacg     1260

atggttgcga tgacgcagat caccaagaag atgcttgcca gcaaggagaa ggccgaggag     1320

ctgtacccag tgaagccgtg gaactgccgc aagttcctgg tgctgtccat cgggacgggg     1380

tcgacgtcgg agcagggcct gtacacggcg cggcagtgct cgcggtgggg catctgccgg     1440

tggatccgga acaacggcat ggcccccatc atcgacatct tcatggcggc gagctcggac     1500

ctggtggaca tccacgtcgc cgcgatgttc cagtcgctcc acagcgacgg cgactacctg     1560

cgcatccagg acaactcgct gcacggcgcc gcggccaccg tggacgcggc gacgccggag     1620

aacatgcgga cgctcgtcgg gatcggggag cggatgctgg cgtagcgggt gtccagggtc     1680

aacgtggaga cagggaggta cgaaccggtg cctggggaag gaagcaacgc tgatgcgctc     1740

gctgggatcg caaggcagct ctcggaggag aggaggacaa ggctcgcgcg ccgcacctcc     1800

gccatcgtca gctccggtgg tgcctctaga cgtacgtgtg cctcaaaggt ctccaatgtc     1860

taagagaagt gaatttgttg cctgattaaa atcttaatta attaattctg tgggctgtgg     1920

ctcaaataaa actatatcat taaaaaaccc gccatgaact agttctagga aaatacgcat     1980

ggttttagaa agaaaaacat aaactaattc taacaaaatg tacacacctc t              2031


<210>  14
<211>  1116
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of Sorghum bicolor phospholipase mutant Q372stop

<400>  14
atggcgacct actactcttc gcggcgtcca tgcaacgcct gcagcacgaa ggcgatggcc       60

gggagcgtgg tcggcgagcc cgtcgtgctg gggcagaggg tgacggtgct gacggtggac      120

ggcggcggca tccgtggtct catccccgga accatccttg ccttcctcga ggcccggctg      180

caggagctgg acgggccgga ggttaggctc gcggactact tcgactacat cgccgggacg      240

agcaccggcg ggctcatcac cgccatgctc accgcgcccg gcaaggacag gcggcctctc      300

tacgctgcca aggacatcaa ccaattctac atggagaatt gccctcgcat cttccctcaa      360

aagagcagca ggcttgcggc cgccatgtcc gcgctgagga agccaaggta caacggcaag      420

tgcctccgta acctgatcat gagcatgctc ggcgagacga gggtgagcga cacgctcacc      480

aacgtcatca tccctacctt cgacgtcagg ctgctgcagc ccatcatctt ctccacctac      540

gacgccaaga gcatgcctct gaagaacgcg ctgctctccg acgtgtgcat cggcacgtcc      600

gccgcgccga cctacctccc ggcgcactac ttccagacca aggacgccgg cagtggcaag      660

gaacgcgagt acaacctcat cgacggcggt gtcgccgcca acaatccgac gatggttgcg      720

atgacgcaga tcaccaagaa gatgcttgcc agcaaggaga aggccgagga gctgtaccca      780

gtgaagccgt ggaactgccg caagttcctg gtgctgtcca tcgggacggg gtcgacgtcg      840

gagcagggcc tgtacacggc gcggcagtgc tcgcggtggg gcatctgccg gtggatccgg      900

aacaacggca tggcccccat catcgacatc ttcatggcgg cgagctcgga cctggtggac      960

atccacgtcg ccgcgatgtt ccagtcgctc cacagcgacg gcgactacct gcgcatccag     1020

gacaactcgc tgcacggcgc cgcggccacc gtggacgcgg cgacgccgga gaacatgcgg     1080

acgctcgtcg ggatcgggga gcggatgctg gcgtag                               1116


<210>  15
<211>  371
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of mutated Sorghum bicolor phospholipase 
       Q372stop

<400>  15

Met Ala Thr Tyr Tyr Ser Ser Arg Arg Pro Cys Asn Ala Cys Ser Thr 
1               5                   10                  15      


Lys Ala Met Ala Gly Ser Val Val Gly Glu Pro Val Val Leu Gly Gln 
            20                  25                  30          


Arg Val Thr Val Leu Thr Val Asp Gly Gly Gly Ile Arg Gly Leu Ile 
        35                  40                  45              


Pro Gly Thr Ile Leu Ala Phe Leu Glu Ala Arg Leu Gln Glu Leu Asp 
    50                  55                  60                  


Gly Pro Glu Val Arg Leu Ala Asp Tyr Phe Asp Tyr Ile Ala Gly Thr 
65                  70                  75                  80  


Ser Thr Gly Gly Leu Ile Thr Ala Met Leu Thr Ala Pro Gly Lys Asp 
                85                  90                  95      


Arg Arg Pro Leu Tyr Ala Ala Lys Asp Ile Asn Gln Phe Tyr Met Glu 
            100                 105                 110         


Asn Cys Pro Arg Ile Phe Pro Gln Lys Ser Ser Arg Leu Ala Ala Ala 
        115                 120                 125             


Met Ser Ala Leu Arg Lys Pro Arg Tyr Asn Gly Lys Cys Leu Arg Asn 
    130                 135                 140                 


Leu Ile Met Ser Met Leu Gly Glu Thr Arg Val Ser Asp Thr Leu Thr 
145                 150                 155                 160 


Asn Val Ile Ile Pro Thr Phe Asp Val Arg Leu Leu Gln Pro Ile Ile 
                165                 170                 175     


Phe Ser Thr Tyr Asp Ala Lys Ser Met Pro Leu Lys Asn Ala Leu Leu 
            180                 185                 190         


Ser Asp Val Cys Ile Gly Thr Ser Ala Ala Pro Thr Tyr Leu Pro Ala 
        195                 200                 205             


His Tyr Phe Gln Thr Lys Asp Ala Gly Ser Gly Lys Glu Arg Glu Tyr 
    210                 215                 220                 


Asn Leu Ile Asp Gly Gly Val Ala Ala Asn Asn Pro Thr Met Val Ala 
225                 230                 235                 240 


Met Thr Gln Ile Thr Lys Lys Met Leu Ala Ser Lys Glu Lys Ala Glu 
                245                 250                 255     


Glu Leu Tyr Pro Val Lys Pro Trp Asn Cys Arg Lys Phe Leu Val Leu 
            260                 265                 270         


Ser Ile Gly Thr Gly Ser Thr Ser Glu Gln Gly Leu Tyr Thr Ala Arg 
        275                 280                 285             


Gln Cys Ser Arg Trp Gly Ile Cys Arg Trp Ile Arg Asn Asn Gly Met 
    290                 295                 300                 


Ala Pro Ile Ile Asp Ile Phe Met Ala Ala Ser Ser Asp Leu Val Asp 
305                 310                 315                 320 


Ile His Val Ala Ala Met Phe Gln Ser Leu His Ser Asp Gly Asp Tyr 
                325                 330                 335     


Leu Arg Ile Gln Asp Asn Ser Leu His Gly Ala Ala Ala Thr Val Asp 
            340                 345                 350         


Ala Ala Thr Pro Glu Asn Met Arg Thr Leu Val Gly Ile Gly Glu Arg 
        355                 360                 365             


Met Leu Ala 
    370     


<210>  16
<211>  2979
<212>  DNA
<213>  Helianthus annuus

<400>  16
atgaataaca tcaatcttgt aatagtttcg cttgtaatcg cgattgtagc catccaaccc       60

cttgcgcaag agcaaaccga tgtaggtgag gcaaatttcg tcactgttct tagcatcgat      120

ggtgggggtg ttcgtggcat tgttcccgcc accttgcttg cttttcttga atccaaaatt      180

caggtactcg aacttaaaat gcacatgtgc atcatattac aagctgtaac ttattattga      240

aatgtgccgt ctcttcggat aggaaataga tgggccagat gcacgaattg cggattattt      300

tgatgtaata gccggaacaa gcacaggagg gctgatgaca actatgcttg cagctcctaa      360

tgagaaaaat cgtcccatgt tcgccgcaaa agacattacc aacttctact ttcaacattc      420

gcctaggatc ttccctaaaa tagggtaaac tctaactagt ttccggatct ataagatcat      480

cattaaatac aagtttcatt ttctttttcg aatcaaatac agacacacat ttgatgaggc      540

gcaaccttat ccttctcaaa acgaagcctg cgaaatgggg tattctccta caaagacttt      600

tgtaattcat gttctagtgg gtgtttggat gtgcgtttta aaactgatta ttatttacat      660

gtagtttctg aagaaaataa aacagttatt caaacacttt ttgttaataa ttctactaga      720

aaaaaaaaat ccttgtcagg aaattaatta aaaaaaagtt accatctatt aaagttcttt      780

cttactaatc aaaagttttt aaattttatt atcatgttat tataactaaa catacacatc      840

caaacactat ctcataccac atgattacac aagtctatta tttgaatatg ctaacttagt      900

attttcatat aataagtttt taaaacgcca catccaaaac ccttgattct tattttacat      960

tgtgtagcta aaacagtgtt tatacataaa aacaatcagt tatataaatc aaagcattat     1020

ttaactaaag taagctcggt tcaaactcga taagagaatt aatatatacg agtcgagttc     1080

ctgttgacca gatttcgcta gtgttaagtt tcgagttcaa aattgtatat gaacttgaac     1140

ctgtgtatca tcatacttga catttaaacc ataatgttgt tgaataaata aagtgatttt     1200

attttgtagt cggaccaaat tcatgaattc ggtagtaacc gtacttggtg aggccaccgg     1260

accaaagtat gatggtaaat atcttcgagc catggcaaag atgatgttaa aaaacctcac     1320

tattaaagat acgttgacga atgttgtcat acctgctttc gacattaggc ggcttcaacc     1380

tgttatcttc tcctctgctc aagtaattaa actcgttttt tatatttata gcagttctct     1440

atttaaaatt gattgtgtat cataaaatgg tttctgtttg atacgtttag ggaaaagagg     1500

tcgcgtggaa aaatgctttg ctagcagacg tatgcattag taccgcggcg gcaccaacat     1560

ttttcccgcc atactatttt gagactagag acgtcgatgg aaccaagcac acttttgatc     1620

taatcgatgg cggggtagct gcaaacaatc cggtagttac atttcaacaa tattgagttt     1680

gcattttatt tttaggacaa gtagtcacat tagggtgaag ggtgtgttca agctcatccc     1740

gaaggtggga gcggtgttcc cactcgtacc tcatggcgca ttttcttctt tgttgcagct     1800

ccaattttaa aaagccaccc cgccttttcc attccatagc gccacgtcaa ctgggaaatg     1860

gtgttcccac tggtattgga gatttggagg cgctacgcca cctctgtcat cccgaagcca     1920

caccctccac ccttaggagt gttatcggtt cagttttcgg tttatacagt ttaaaggttt     1980

ttttttggtt gaaaccaaaa accgaactga actgaacgga attcgggtag ttcacaactg     2040

aaccaaaaac tgaatccata ttcggttttc tgtttgaccg aataatcatt atttgttatt     2100

tgattgtgcg aggttaaata agattgagca aaaattgtaa ttaattggat ttggctaaat     2160

gacaatttaa ataaccgtta tttgttatcc gattcaaacc gaacacccaa ctcgatttgg     2220

tttaataccg aaccgacaac cggatacgta attcagttcg ctattaaaag gttcgggttt     2280

ggtagggttt aacccatttg aaatcgaatc ttcagagaaa gggttcgcta atgtcaccca     2340

cataaccaaa gaaatcttgt ttaaatgtta atggcagaca catttggcta tcacacatat     2400

aaccaaagaa gcggtgatgg ggaaatacag gttctctggc ccggaggttt tcgacggaag     2460

acggatgctt gtgctttcac tcggcactgg tacgcagacg tacaatgact tatatactgc     2520

acaaaaggct gcaaaatggg ggttgcttag ttggatcttt accaatggta ctgcgccaat     2580

cctccgcatt tttggtgatg ccatgtcaga tatggtcgac atccatgtgt caactatatt     2640

ccaatcgttg caagtcgaaa aaaactatct gcgtattcag gtataactaa gaacatataa     2700

atataatgtt gtataggtta catgtttagt aacaaggagt ttttttatgg gcaggaagat     2760

aacttgaaag gggaagcaac tgcaatggat atttcatcac ctgagaacat gagggcgcta     2820

gaggacattg gcaagaaatt gttgaagaaa ccgttgtcga gattggatgt ggagacaggc     2880

aagcttgaac cagttaaagg agaaggtacg aatgctgatg cattagcacg tttcgccact     2940

ttgctttgtg ccgaacgaaa gcgccgcaat ccagcttaa                            2979


<210>  17
<211>  1305
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of Helianthus annuus phospholipase

<400>  17
atgaataaca tcaatcttgt aatagtttcg cttgtaatcg cgattgtagc catccaaccc       60

cttgcgcaag agcaaaccga tgtaggtgag gcaaatttcg tcactgttct tagcatcgat      120

ggtgggggtg ttcgtggcat tgttcccgcc accttgcttg cttttcttga atccaaaatt      180

caggaaatag atgggccaga tgcacgaatt gcggattatt ttgatgtaat agccggaaca      240

agcacaggag ggctgatgac aactatgctt gcagctccta atgagaaaaa tcgtcccatg      300

ttcgccgcaa aagacattac caacttctac tttcaacatt cgcctaggat cttccctaaa      360

ataggacaca catttgatga ggcgcaacct tatccttctc aaaacgaagc ctgcgaaatg      420

ggtcggacca aattcatgaa ttcggtagta accgtacttg gtgaggccac cggaccaaag      480

tatgatggta aatatcttcg agccatggca aagatgatgt taaaaaacct cactattaaa      540

gatacgttga cgaatattgt catacctgct ttcgacatca ggcggcttca acctgttatc      600

ttctcctctg ctcaaggaaa agaggtcgcg tggaaaaatg ctttgctagc agacgtatgc      660

attagtaccg cggcggcacc aacgtttttc ccgccatact attttgagac tagagatgtc      720

gatggaacca agcacacttt tgatctaatc gatggcgggg tagctgcaaa caatccgaca      780

catttggcta tcacacatat aaccaaagaa gcggtgatgg ggaaatacag gttctctggc      840

ccggaggttt tcgacggcag acggatgctt gtgctttcac tcggcactgg tacgcagacg      900

tacaatgact tatacactgc acaaaaggct gcaaaatggg ggttgcttag ttggatcttt      960

accaatggta ctgcgccaat cctccgcatt tttggtgatg ccatgtcaga tatggtcgac     1020

atccatgtgt caactatatt ccaatcgttg caagtcgaaa aaaactatct gcgtattcag     1080

gaagataact tgaaagggga agcaactgca atggatattt catcacccga gaacatgagg     1140

gcgctagagg acattggcaa gaaattgttg aagaaaccgt tgtcgagatt ggatgtggag     1200

acaggcaagc ttgaaccagt taaaggagaa ggtacgaatg ctgatgcatt agcacgtttc     1260

gccactttgc tttgtgccga acgaaagcgc cgcaatccag cttaa                     1305


<210>  18
<211>  434
<212>  PRT
<213>  Helianthus annuus

<400>  18

Met Asn Asn Ile Asn Leu Val Ile Val Ser Leu Val Ile Ala Ile Val 
1               5                   10                  15      


Ala Ile Gln Pro Leu Ala Gln Glu Gln Thr Asp Val Gly Glu Ala Asn 
            20                  25                  30          


Phe Val Thr Val Leu Ser Ile Asp Gly Gly Gly Val Arg Gly Ile Val 
        35                  40                  45              


Pro Ala Thr Leu Leu Ala Phe Leu Glu Ser Lys Ile Gln Glu Ile Asp 
    50                  55                  60                  


Gly Pro Asp Ala Arg Ile Ala Asp Tyr Phe Asp Val Ile Ala Gly Thr 
65                  70                  75                  80  


Ser Thr Gly Gly Leu Met Thr Thr Met Leu Ala Ala Pro Asn Glu Lys 
                85                  90                  95      


Asn Arg Pro Met Phe Ala Ala Lys Asp Ile Thr Asn Phe Tyr Phe Gln 
            100                 105                 110         


His Ser Pro Arg Ile Phe Pro Lys Ile Gly His Thr Phe Asp Glu Ala 
        115                 120                 125             


Gln Pro Tyr Pro Ser Gln Asn Glu Ala Cys Glu Met Gly Arg Thr Lys 
    130                 135                 140                 


Phe Met Asn Ser Val Val Thr Val Leu Gly Glu Ala Thr Gly Pro Lys 
145                 150                 155                 160 


Tyr Asp Gly Lys Tyr Leu Arg Ala Met Ala Lys Met Met Leu Lys Asn 
                165                 170                 175     


Leu Thr Ile Lys Asp Thr Leu Thr Asn Ile Val Ile Pro Ala Phe Asp 
            180                 185                 190         


Ile Arg Arg Leu Gln Pro Val Ile Phe Ser Ser Ala Gln Gly Lys Glu 
        195                 200                 205             


Val Ala Trp Lys Asn Ala Leu Leu Ala Asp Val Cys Ile Ser Thr Ala 
    210                 215                 220                 


Ala Ala Pro Thr Phe Phe Pro Pro Tyr Tyr Phe Glu Thr Arg Asp Val 
225                 230                 235                 240 


Asp Gly Thr Lys His Thr Phe Asp Leu Ile Asp Gly Gly Val Ala Ala 
                245                 250                 255     


Asn Asn Pro Thr His Leu Ala Ile Thr His Ile Thr Lys Glu Ala Val 
            260                 265                 270         


Met Gly Lys Tyr Arg Phe Ser Gly Pro Glu Val Phe Asp Gly Arg Arg 
        275                 280                 285             


Met Leu Val Leu Ser Leu Gly Thr Gly Thr Gln Thr Tyr Asn Asp Leu 
    290                 295                 300                 


Tyr Thr Ala Gln Lys Ala Ala Lys Trp Gly Leu Leu Ser Trp Ile Phe 
305                 310                 315                 320 


Thr Asn Gly Thr Ala Pro Ile Leu Arg Ile Phe Gly Asp Ala Met Ser 
                325                 330                 335     


Asp Met Val Asp Ile His Val Ser Thr Ile Phe Gln Ser Leu Gln Val 
            340                 345                 350         


Glu Lys Asn Tyr Leu Arg Ile Gln Glu Asp Asn Leu Lys Gly Glu Ala 
        355                 360                 365             


Thr Ala Met Asp Ile Ser Ser Pro Glu Asn Met Arg Ala Leu Glu Asp 
    370                 375                 380                 


Ile Gly Lys Lys Leu Leu Lys Lys Pro Leu Ser Arg Leu Asp Val Glu 
385                 390                 395                 400 


Thr Gly Lys Leu Glu Pro Val Lys Gly Glu Gly Thr Asn Ala Asp Ala 
                405                 410                 415     


Leu Ala Arg Phe Ala Thr Leu Leu Cys Ala Glu Arg Lys Arg Arg Asn 
            420                 425                 430         


Pro Ala 
        


<210>  19
<211>  1531
<212>  DNA
<213>  Hordeum vulgare

<400>  19
atggcaagct actggtgccg gcgaccgtgc gagtcgtgca gcacgagggc gatggcgggc       60

agcgtggtcg gccagccggt ggcgccgggg cagagggtga cggtgctgac catcgacggc      120

ggcggcatcc gcggcctcat cccgggcacc atcctcgcct tcctcgaggc caggctgcag      180

gagctggacg ggccggacgc gcgcctggcc gactacttcg actgcatcgc cggcaccagc      240

accggcgggc tcatcaccgc catgctcacc gcgcccggcc aggacggccg cccgctcttc      300

gccgccaagg acgtcaaccg cttctacctc gacaacgggc cctacatctt cccccaaagg      360

cgagagcacg agcgatctca tggccatgga tcatgccagc tgaaccggtg attgatgtgt      420

gaattgtgtt gagctgtgcc aggaggtgcg cgctcgccgc ggtgaccgcg tcgctgaggc      480

ggccgaggta cagcggcaag tacctgcacg gcaagatcag gagcatgctg ggcgagacga      540

ggctgtgcga cgcgctcacc gacgtcgtca tccccacctt cgacgtcaag cttctccagc      600

ccatcatctt ctccacatac gacgtatgct aatttacgcc attgatcaga tagatccatc      660

ctccttggat cagacagaca tggggctaaa atcgttgaag gatcatgcgt gcaggccagg      720

aacatgcccc tgaagaacgc gcggcttgcc gacatctgca tcggcacctc cgccgccccg      780

acctacctcc cggcgcacca cttccacacc caagacgaca acggtaagga gcgcgagtac      840

aacctcatcg acggcggcgt cgccgccaac aatccggtaa ccaaccaatc aagcgtccat      900

cagtcatcac atattcagat acgctgcccg agcacactga tgaactgagt tgtgagagat      960

gcagacgatg gtgaccatga cgcagatcac caagaagatg atggtcaagg acagggagga     1020

gctgtacccg gtgaagccgt cggactgcgg caagttcctg gtgctgtcca tcgggaccgg     1080

ctcgacgtcg gaccaggggc tgtacacggc caagcagtgc tcccagtggg gcatcatccg     1140

ctggctgcgc aacaagggca tggcgcccat catcgacatc ttcatggcgg ccagctccga     1200

cctcgtcgac atccacgccg ccgtgctctt ccagtcgctg cacagcgacg gtaactacct     1260

ccgcatccag gacaactcgc tccacggccc agccgcgacg gtggacgccg ccacgcccga     1320

gaacatggcg gagctcctca ggatcggcga gcggatgttg gcacagaggg tgtccagggt     1380

gaacgtcgag accgggaggt acgaggagat acggggcgcc gggagcaacg ccgacgcgct     1440

cgccggcttc gccaaacagc tctccgacga gaggaggaca aggctcggcc gccggcgcgt     1500

tggcgccggc cgtctgaaat ccagacgctg a                                    1531


<210>  20
<211>  1269
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of Hordeum vulgare phospholipase

<400>  20
atggcaagct actggtgccg gcgaccgtgc gagtcgtgca gcacgagggc gatggcgggc       60

agcgtggtcg gccagccggt ggcgccgggg cagagggtga cggtgctgac catcgacggc      120

ggcggcatcc gcggcctcat cccgggcacc atcctcgcct tcctcgaggc caggctgcag      180

gagctggacg ggccggacgc gcgcctggcc gactacttcg actgcatcgc cggcaccagc      240

accggcgggc tcatcaccgc catgctcacc gcgcccggcc aggacggccg cccgctcttc      300

gccgccaagg acgtcaaccg cttctacctc gacaacgggc cctacatctt cccccaaagg      360

aggtgcgcgc tcgccgcggt gaccgcgtcg ctgaggcggc cgaggtacag cggcaagtac      420

ctgcacggca agatcaggag catgctgggc gagacgaggc tgtgcgacgc gctcaccgac      480

gtcgtcatcc ccaccttcga cgtcaagctt ctccagccca tcatcttctc cacatacgac      540

gccaggaaca tgcccctgaa gaacgcgcgg cttgccgaca tctgcatcgg cacctccgcc      600

gccccgacct acctcccggc gcaccacttc cacacccaag acgacaacgg taaggagcgc      660

gagtacaacc tcatcgacgg cggcgtcgcc gccaacaatc cgacgatggt gaccatgacg      720

cagatcacca agaagatgat ggtcaaggac agggaggagc tgtacccggt gaagccgtcg      780

gactgcggca agttcctggt gctgtccatc gggaccggct cgacgtcgga ccaggggctg      840

tacacggcca agcagtgctc ccagtggggc atcatccgct ggctgcgcaa caagggcatg      900

gcgcccatca tcgacatctt catggcggcc agctccgacc tcgtcgacat ccacgccgcc      960

gtgctcttcc agtcgctgca cagcgacggt aactacctcc gcatccagga caactcgctc     1020

cacggcccag ccgcgacggt ggacgccgcc acgcccgaga acatggcgga gctcctcagg     1080

atcggcgagc ggatgttggc acagagggtg tccagggtga acgtcgagac cgggaggtac     1140

gaggagatac ggggcgccgg gagcaacgcc gacgcgctcg ccggcttcgc caaacagctc     1200

tccgacgaga ggaggacaag gctcggccgc cggcgcgttg gcgccggccg tctgaaatcc     1260

agacgctga                                                             1269


<210>  21
<211>  422
<212>  PRT
<213>  Hordeum vulgare

<400>  21

Met Ala Ser Tyr Trp Cys Arg Arg Pro Cys Glu Ser Cys Ser Thr Arg 
1               5                   10                  15      


Ala Met Ala Gly Ser Val Val Gly Gln Pro Val Ala Pro Gly Gln Arg 
            20                  25                  30          


Val Thr Val Leu Thr Ile Asp Gly Gly Gly Ile Arg Gly Leu Ile Pro 
        35                  40                  45              


Gly Thr Ile Leu Ala Phe Leu Glu Ala Arg Leu Gln Glu Leu Asp Gly 
    50                  55                  60                  


Pro Asp Ala Arg Leu Ala Asp Tyr Phe Asp Cys Ile Ala Gly Thr Ser 
65                  70                  75                  80  


Thr Gly Gly Leu Ile Thr Ala Met Leu Thr Ala Pro Gly Gln Asp Gly 
                85                  90                  95      


Arg Pro Leu Phe Ala Ala Lys Asp Val Asn Arg Phe Tyr Leu Asp Asn 
            100                 105                 110         


Gly Pro Tyr Ile Phe Pro Gln Arg Arg Cys Ala Leu Ala Ala Val Thr 
        115                 120                 125             


Ala Ser Leu Arg Arg Pro Arg Tyr Ser Gly Lys Tyr Leu His Gly Lys 
    130                 135                 140                 


Ile Arg Ser Met Leu Gly Glu Thr Arg Leu Cys Asp Ala Leu Thr Asp 
145                 150                 155                 160 


Val Val Ile Pro Thr Phe Asp Val Lys Leu Leu Gln Pro Ile Ile Phe 
                165                 170                 175     


Ser Thr Tyr Asp Ala Arg Asn Met Pro Leu Lys Asn Ala Arg Leu Ala 
            180                 185                 190         


Asp Ile Cys Ile Gly Thr Ser Ala Ala Pro Thr Tyr Leu Pro Ala His 
        195                 200                 205             


His Phe His Thr Gln Asp Asp Asn Gly Lys Glu Arg Glu Tyr Asn Leu 
    210                 215                 220                 


Ile Asp Gly Gly Val Ala Ala Asn Asn Pro Thr Met Val Thr Met Thr 
225                 230                 235                 240 


Gln Ile Thr Lys Lys Met Met Val Lys Asp Arg Glu Glu Leu Tyr Pro 
                245                 250                 255     


Val Lys Pro Ser Asp Cys Gly Lys Phe Leu Val Leu Ser Ile Gly Thr 
            260                 265                 270         


Gly Ser Thr Ser Asp Gln Gly Leu Tyr Thr Ala Lys Gln Cys Ser Gln 
        275                 280                 285             


Trp Gly Ile Ile Arg Trp Leu Arg Asn Lys Gly Met Ala Pro Ile Ile 
    290                 295                 300                 


Asp Ile Phe Met Ala Ala Ser Ser Asp Leu Val Asp Ile His Ala Ala 
305                 310                 315                 320 


Val Leu Phe Gln Ser Leu His Ser Asp Gly Asn Tyr Leu Arg Ile Gln 
                325                 330                 335     


Asp Asn Ser Leu His Gly Pro Ala Ala Thr Val Asp Ala Ala Thr Pro 
            340                 345                 350         


Glu Asn Met Ala Glu Leu Leu Arg Ile Gly Glu Arg Met Leu Ala Gln 
        355                 360                 365             


Arg Val Ser Arg Val Asn Val Glu Thr Gly Arg Tyr Glu Glu Ile Arg 
    370                 375                 380                 


Gly Ala Gly Ser Asn Ala Asp Ala Leu Ala Gly Phe Ala Lys Gln Leu 
385                 390                 395                 400 


Ser Asp Glu Arg Arg Thr Arg Leu Gly Arg Arg Arg Val Gly Ala Gly 
                405                 410                 415     


Arg Leu Lys Ser Arg Arg 
            420         


<210>  22
<211>  9001
<212>  DNA
<213>  Beta vulgaris


<220>
<221>  misc_feature
<222>  (2702)..(2702)
<223>  n is a, c, g, or t

<400>  22
ttttccaaca acataccgag tagttttctc caagtttaag ttatttatgt tattgtaatt       60

gtaatgtaag agctagtaaa gctcagttta gtttctttgt ttttagtatg aatgtaccat      120

ttttagtatg taattatgct tttaattaat gcaaaatttt ccgttgtaaa aaaaagtcta      180

catgtccctc ttgcagagat aaatgtaagg gacattattg cttagttctc gactaattta      240

caataagttt ataattggtt atcatggttc atgagtactc ctgttcatag aaggacaata      300

atttataaag gtttcttagt agcttccaat gacggagctc aatgaccctg ttaattagct      360

aagtaataag ttggccatta gccaataaca actgtattat tagctagtaa gttcattaga      420

tcgatataac ttataagtct aatgaatata aatatgaata agatggaact gaaatgagtt      480

ttatatttga aaaattagtg gcatacgtgc ttaaacgttt ttttgattat ggctagaaga      540

cttagaacga agttggagga attcttcatt catataaatt tatgctagat aaactaatag      600

ccagtcaagt caagtttatg tttttaaaaa aaatcttatc tcatgtagta cgagaatctt      660

tattttataa tttcgttata ataaatatgc atttcactcc tttcaaatcg ttagctacat      720

cacttctgca accaagcaag caagccataa tatttaatca caggttcttt aatactatag      780

tctatatata catgcaatcc acttacatta attatcatct tattctccca tcatcaagaa      840

aacacattac aatccaaaga aataaaacat ggagaattct agatctctgg gtaaccctgt      900

tagccctaga ccccctaatc atggtaatct cataacaatt ttaagtattg atgggggagg      960

cattagggga ataatccctg cagttatgct tgaattcctt gaatcacaac tccaggtatg     1020

tacataatta agaaatgatg atatacgatc atctttagta ctcttgttaa atgatttgac     1080

gtaagaaatc atgtatatat tatgcatgcc ttactataat attgcgtgca tgttcataat     1140

cacaacagaa gttagatggt gaggaagcaa gattggctga ttattttgat gttattgccg     1200

gaacgagcac cggtggcctc ataactgcca tgttaaccgc ccctaacgac aaaactcggc     1260

cactctacgc cgccaaggag attactcctt tttatcttga gcattgtccg aagatcttcc     1320

gccaaccaag gtaaataaat caaatttatc atctatgtat atcaggattg gtaaattaga     1380

gaggatgaaa aacccttaaa tgcttggaac tattaacgct cgagttccaa ttatgggcca     1440

ttgtctctta taggtctcaa ctattgattt ttttctatag caggcgcgga tgtaagattt     1500

tgaatttgga agggtcctaa ctaaaccaaa gaaataaatt acaatgacca tattaacata     1560

taagaagttt agttttaaca ataagaaaac caacagttgg tcttgtaaga gaccgtctac     1620

ttttaattta aaagtaggcg cggcccaagc ccaatattaa gaagagaata gatcatattt     1680

cttaatatag tgactacttt aatctattta gtgaccattt taatctattt agtgaccagt     1740

ttaatctatt cattaataac attctttaat tcatttagtg actgatttaa tccatttagt     1800

gactagctta atccattttg taagactgac ggactatttt taaaaattgt ttgttctaat     1860

tcaaaattta gaagggaaat aaaagaagga gaagagaatg gacgactagg atggtgcaag     1920

atacctaaaa aaagtgttag gttagagaga aattgaatgg gcttcttagg agaatgtagg     1980

tttgcattgt ctaattttat attaaaatta gaccgtctat tataataatt tgtggaaaac     2040

aaaactttat attatttgaa tcatgtatta gatatacctt aattatattg atatcttaat     2100

gttatcttca tttttagtaa attcaacaga tttgatttgt acatccgtta acatatgcga     2160

cactaacatt tgatatcttc agttatatgt tcataatttt tttttttaaa aagttggaca     2220

taaatttggt aaacgaaaat agagacaaat tcacaatttt acatagtaat attattttca     2280

agtatttgga aacaagcttg gtcaaactta atatagcaat accgtagaaa gtcaaattat     2340

tgaatttatt tgaaacaaaa ggagaataat cgttatatca caaaaaaaaa agtattaaaa     2400

ttagagctaa attaagagag attggtagca ttcaagataa tgaaatgatg attagatata     2460

aaaaagaaaa gatgataaaa gagaaaagtt aaagaacagg aagggcatgt tctcttcagc     2520

ataaaacagc tgaactgaac taacctgaat tgaactgatt ggagctgaac tgaactgaac     2580

tgaactgaac tgaattgaat ttaatttaat ttaattgtat tgaattaaat taaatttaat     2640

ttaatttaat tgaattgaat taaattgaat gtaataaata tataaataat atttaaacaa     2700

tnagtacagt tattataata ataattgtgc ccttctgaaa ttgttaaggc acgtcgtttg     2760

caagatttgt ccttttgcat taaaatttgg gtgcaaatcc tgcacaaatt gaaatatata     2820

gtattatctc tccactcatt tttatcttct tgtatcattt taggctcgac aataatgact     2880

ctgccagagg ggtagttgaa ccttcttata tcatcatcat aattcataat aataatttta     2940

ttatatatac tccctccgtt ccaaaatata gttctcgttt ctttttttta cgatgatttg     3000

tgcaagtaga atctaagtgg aataataaaa tttttttgtt tatttaaata aatattgtat     3060

ggaaaaggat gattttagga gagaaaaatg gaaaataatt ggtgaaagag tattaattgt     3120

aatattttag ttgaataaat aacagaaaaa ccaaaatata agaagcaaat aaatgatggg     3180

acacgatctt tgtagacaaa ttacagaaaa atttggaaac gaatatggaa atgggaacta     3240

tattttggga cacccaaata gaaatgggaa ctatattttg ggacggaggg agtaatatta     3300

ataatcataa attattaata ataatagtaa taattaatga tactaatgat aattaataac     3360

cgaagatcgc gtgattattt gtgttggagt tgtagtggga tatttggttc aatagggact     3420

ctaatgaagc tgctaagcgg gccgaaatac gatggaaagt accttcacaa tctagtaaag     3480

gaattgctcg gacaaagaag gttgcatcaa gcgttgacta atgtggttat cccaactttt     3540

gatatcaaga atcttcagcc tgttctcttt tctacttata tggtatgttg ttgaatttcc     3600

aatttattga agtacattat tattgtaccc ttggttatct tattcattag caaatactag     3660

cattcaaata gcatttatag tgatttgtca agaaaaaaaa taagtagagg aatcatagtt     3720

atcacttatt agaactacct cttttagaag aatatacttc ctctattttt ttagatggaa     3780

catttacctt tttggtgagt agattaaaat aatcatatgt tgtatttaaa aaaacagaat     3840

tagtatatat cttcttttgt gtcatataat acacttgatc aatttaaaag ataacatgat     3900

tccaatattg acttttttat acacataatt tcacatgtca aataatagaa aaaaagtcaa     3960

ctttgctgaa tttggagatg ataatacata gtttatgtgt tggttaagta atttcatact     4020

actacattga tagattatga aattgatcaa gaaaactaat caataattaa tgattataat     4080

gatctaggtc ccaattgcta aagaattgga cgtgttgctg tcagacatat gcataggcac     4140

gtctgcagct ccaacgttcc taccagcgca tcattttgaa aacaaagatg atcagggcaa     4200

tgtcaaagaa ttcaacctca ttgatggtgg tattgctgcc aataatcctg taagattatt     4260

tcttcatcca tgtttggatt ttctctccat ccctaaattt aatttgatta ggaattagtc     4320

attcttaata aaaattatga atcatcaatt aaattatcta ataaaaagta attcaaacat     4380

ttgttcgata taatgtactt atttttttta ttaaatttta attttcagac atattccaaa     4440

tgtcgtccta caaaagaaat tgcaaatcca tctaaaaact tcgtttgata catctaataa     4500

tgtatgttcg agtcaatcat taaattttca tatcaagata aatatcaaag taagatgttt     4560

caaactttta cttagtcgat taattctatt aattccagtc aatttaccat caacttcttc     4620

ggtcttccca aaacaaatgg aaaaatatat attcactgca tacgtgtctt tgtgttatat     4680

tcctatctct aaagtattta agtttttatg atattaggac atatatattc tctagtattt     4740

aatgagaaag atcgaaaagt tttcgtatat tcataaaacc ttaacaactg ctattcaaaa     4800

tatacgataa gtttcatcaa cattacccac taattaaatg taatttcact gtataagtac     4860

agtcattagt ggcaataagc gaagtgacaa aacaaatagt gaagagcaac ccgaatttct     4920

tcccgataaa ggccacggat cgtgaacggt tgctggtgat atcgttaggg actggatccg     4980

acaaggtaga gcagctctat aatgctaaaa gcgcagcaaa gtggggaatt atttcatggt     5040

tatttgacaa tggtaatact ccactacttg atgcttttaa tcaatcaaag gctgatatgg     5100

tcgatttcca caattctgtg gcctttcaag catatggttc tcttgacaat tatcttcgca     5160

tccaagtaag ttatttttat tatacggatt tggatgttta tgtacgtata tatacttctg     5220

ttaaaggttg ataagatgag agttacgcgc tcattttacc aaggatcttt ggtttcaatg     5280

gtaatttggg accggtcaga cccgtggctg cgggcctatc acggtcaaga atttttattt     5340

caggctcgca ccgtcacgag cctgcctaga tggctcgcga gccgaacact catttccttt     5400

gctttttagc ctaaaactgt caaaagtcca aaaaacgaca taaaacacgg gccagctcgc     5460

aggagccacg agcccagtcc gtggataaaa tcttgaaatt tcaggcccga cccaccattt     5520

ccccaaaccc gcgggccgag cccgtgaaca agctcatgcc ctgatgccca ggtctaggtc     5580

tgcctcgcac ggagccttca tcccgaagcc cattacaagc cagacaccca actccctaca     5640

acaccacatc aacccttcca atccactcgc cttccctctt cactgctgcc ggctgccctc     5700

taggtttatc attttttcta agctcttttg tctaagttaa atccactggt ttctttcatt     5760

ttctttttga tttaagttcg attaccaatg tttctttaac tttgcaactt ttggggagat     5820

accttattgt aatttgctct aatgaaagtt tcttatgctt ataataatgc tcaattgtct     5880

ttcttgatct agtaatctga gagttatcca gttttaaata gttagagcta gtttcactaa     5940

tggtggcaat aagggtgtgg cagggggcga tgcttggttt tggactttgg taatggtggt     6000

aatgaaggga ggttacacag tgaggatggt ggttggtgta attggtgggg aaactcgggg     6060

tagagagata acccctaaag gcgagattat gcttagtcaa tcgaaaggtg tgcctatttt     6120

aagcggacca cctaaaggtc ggattattat aatcaatttt tcctaaataa attgcaattt     6180

gtggttggat ttctagtttg aaactaaatc tcaattgcaa gtctgcaacc gtatgaacta     6240

tgaaccattt tgaggttaat atatctatag atttttattt caaaaaaata taaaaatata     6300

ttatttcaaa aaggtccaag aatacgtctt ttaagagtgt ccaagaacat ctaaccacga     6360

gttatatatt tgtctattat gtggtgatca ctttttcaag atttgaggtc tttaattagg     6420

ccttgatgat atagatttgt tagagaaaaa catttgcttg attaaaaaag gaaataaatg     6480

atatgacaaa tgactaattg actcatgtct aaccttttaa atgtggtgtg atttaactcg     6540

acatttaata tgtggttggt ttgataaatt ttagcgagca cgctttatat aagtgtaact     6600

tatatatctt gtgcataagt ttagttattt attttttact ttattaaaca aaattgataa     6660

atatgatata gttaattaaa tatattactc tcataaatca agctacccta aatctgtaaa     6720

tgcaaattat attgcaggat gatacactaa agggagtatc agcatcagtc gatgtggcaa     6780

ccacagaaaa tttggcaaat cttgtcacaa ttggaaaggc actcttgaaa aagccagtat     6840

ccaggattaa tttcgatacg ggtagatatc aacctatccc taatggaggg accaatgagg     6900

aagccttaat taggtacttg atttaaataa atcattcata aagaatcacg ctcattgctt     6960

gtcaataaaa tatttatgtt tatgtttaat actaatgctt gttttctaat acttttaaca     7020

ctttactttc ttacatgaga tgccgagacg agtgtagagt aatttattgt gtaggtaact     7080

ttttgctcta tatgagtcac ttatataaaa gtgacatata tatggtaata agttacctgt     7140

aagacgaggt taactatttg ccataaatag gtcactttac ttatatagaa gtgactcatt     7200

taagattaaa attaagttac cttttattta aaattgaccc atattgattt gaaaagtgac     7260

ccatattgat ttgaaaagtg acccatttaa gctttaagtg aactgatgtg acaataaatt     7320

tattatacac ccggtgcaca taatacaaat tgtttaaatt atcaagggtt tgttggacaa     7380

aagtaattgt tagttgtaag cttctagcta tgagtttgtg agcttttaga tgcctaggta     7440

gtttctaact tttagttatg aaactagttg tctaaagtgt ttggtaataa taattattga     7500

cgaaatttta acatgtaaat aatatttaaa tttggcaact tttgataaaa gctaccgaaa     7560

agcttaatca tcttagattt tgaccaagac tactcaaaag ctaaaagtta tctaaaagtt     7620

acatccaaaa gctactttga ggaatattta aacaattttt tcaaagacta aaagctatct     7680

taactacata aaaagtatgt caaaagttac accaaacatg tcctacattg accacgttat     7740

tctaacctac aaaactgact gggcaaacct gctactttgc aagaggtatc agtttattag     7800

ggtgtttcat cttacctacc taaacagtaa aagtaaatat ataaataaaa caaatattaa     7860

ttttatataa atattttatg agattagtat taatgtatat cctataatat caacattcaa     7920

aaaactttta cgaatagata aaaagatata ttaagaatta aaattatgca aattcaaaag     7980

tcaattcaaa ttactcctat taaaaattgg cggtatatga tttaaacttt caaaaataag     8040

aaaattataa aattatgtca ctctttgtta ctactgaata catgtttgtc caattgacag     8100

gtttgcgaaa ctccttaccg aggaaaagag cagacgcgaa caatatcgga cgaaccgaag     8160

tgactaatat cacatttttt aaacccatgt ttcaatggat actatgatta tatagtatat     8220

atatatgtat acaatacatc atgatctgga aaaaaactta cgctcgattt atgataaata     8280

aatttgtcaa accatgtagc tctcacaaaa tctgtaatat ttaatgaatt ttagtatgcg     8340

tagatatggc ctatgggcgt ggaatattta tccatgtaac tcatttattt ttacagctaa     8400

gtcattgcat tttaatctac gaaatttctc ttttaatcaa taacctaaaa ataaatagtt     8460

tgtgcatcaa tttctctcta tttactctgt aagtatttgt tgtttttctt tattgaacga     8520

taactacagt gccactccta tatatacaag agatcacttt ctaaacctag ggataactat     8580

acaagaagag aaactaaccg ttaaaatctt tctacgatca ataaaaggag gaatatataa     8640

tcacatgaaa gtgcgaaata atcaatacaa gtgagtttaa ttccctccgc cactctaact     8700

catttcccta ctctacactt aattctccca cgctatcaat cccattcatt tactccagta     8760

tcagatgcta gtaattcttc atagaatatg aaatatacta ggattatatt ttgcgtttgg     8820

atatagtttt ggtagaataa caattctact ctagttacgt tttctactat tattgggata     8880

tcctttagaa tgtaacctat aaaatatgat caataaaaac actcaaatca aggaagttta     8940

cgcaaatata gcctggtatc aaagctatac taatcttaaa gattattttt tccttcgatc     9000

c                                                                     9001


<210>  23
<211>  1224
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of Beta vulgaris phospholipase 1

<400>  23
atggagaatt ctagatctct gggtaaccct gttagcccta gaccccctaa tcatggtaat       60

ctcataacaa ttttaagtat tgatggggga ggcattaggg gaataatccc tgcagttatg      120

cttgaattcc ttgaatcaca actccagaag ttagatggtg aggaagcaag attggctgat      180

tattttgatg ttattgccgg aacgagcacc ggtggcctca taactgccat gttaaccgcc      240

cctaacgaca aaactcggcc actctacgcc gccaaggaga ttactccttt ttatcttgag      300

cattgtccga agatcttccg ccaaccaagt gggatatttg gttcaatagg gactctaatg      360

aagctgctaa gcgggccgaa atacgatgga aagtaccttc acaatctagt aaaggaattg      420

ctcggacaaa gaaggttgca tcaagcgttg actaatgtgg ttatcccaac ttttgatatc      480

aagaatcttc agcctgttct cttttctact tatatggtcc caattgctaa agaattggac      540

gtgttgctgt cagacatatg cataggcacg tctgcagctc caacgttcct accagcgcat      600

cattttgaaa acaaagatga tcagggcaat gtcaaagaat tcaacctcat tgatggtggt      660

attgctgcca ataatccttc attagtggca ataagcgaag tgacaaaaca aatagtgaag      720

agcaacccga atttcttccc gataaaggcc acggatcgtg aacggttgct ggtgatatcg      780

ttagggactg gatccgacaa ggtagagcag ctctataatg ctaaaagcgc agcaaagtgg      840

ggaattattt catggttatt tgacaatggt aatactccac tacttgatgc ttttaatcaa      900

tcaaaggctg atatggtcga tttccacaat tctgtggcct ttcaagcata tggttctctt      960

gacaattatc ttcgcatcca agatgataca ctaaagggag tatcagcatc agtcgatgtg     1020

gcaaccacag aaaatttggc aaatcttgtc acaattggaa aggcactctt gaaaaagcca     1080

gtatccagga ttaatttcga tacgggtaga tatcaaccta tccctaatgg agggaccaat     1140

gaggaagcct taattaggtt tgcgaaactc cttaccgagg aaaagagcag acgcgaacaa     1200

tatcggacga accgaagtga ctaa                                            1224


<210>  24
<211>  407
<212>  PRT
<213>  Beta vulgaris

<400>  24

Met Glu Asn Ser Arg Ser Leu Gly Asn Pro Val Ser Pro Arg Pro Pro 
1               5                   10                  15      


Asn His Gly Asn Leu Ile Thr Ile Leu Ser Ile Asp Gly Gly Gly Ile 
            20                  25                  30          


Arg Gly Ile Ile Pro Ala Val Met Leu Glu Phe Leu Glu Ser Gln Leu 
        35                  40                  45              


Gln Lys Leu Asp Gly Glu Glu Ala Arg Leu Ala Asp Tyr Phe Asp Val 
    50                  55                  60                  


Ile Ala Gly Thr Ser Thr Gly Gly Leu Ile Thr Ala Met Leu Thr Ala 
65                  70                  75                  80  


Pro Asn Asp Lys Thr Arg Pro Leu Tyr Ala Ala Lys Glu Ile Thr Pro 
                85                  90                  95      


Phe Tyr Leu Glu His Cys Pro Lys Ile Phe Arg Gln Pro Ser Gly Ile 
            100                 105                 110         


Phe Gly Ser Ile Gly Thr Leu Met Lys Leu Leu Ser Gly Pro Lys Tyr 
        115                 120                 125             


Asp Gly Lys Tyr Leu His Asn Leu Val Lys Glu Leu Leu Gly Gln Arg 
    130                 135                 140                 


Arg Leu His Gln Ala Leu Thr Asn Val Val Ile Pro Thr Phe Asp Ile 
145                 150                 155                 160 


Lys Asn Leu Gln Pro Val Leu Phe Ser Thr Tyr Met Val Pro Ile Ala 
                165                 170                 175     


Lys Glu Leu Asp Val Leu Leu Ser Asp Ile Cys Ile Gly Thr Ser Ala 
            180                 185                 190         


Ala Pro Thr Phe Leu Pro Ala His His Phe Glu Asn Lys Asp Asp Gln 
        195                 200                 205             


Gly Asn Val Lys Glu Phe Asn Leu Ile Asp Gly Gly Ile Ala Ala Asn 
    210                 215                 220                 


Asn Pro Ser Leu Val Ala Ile Ser Glu Val Thr Lys Gln Ile Val Lys 
225                 230                 235                 240 


Ser Asn Pro Asn Phe Phe Pro Ile Lys Ala Thr Asp Arg Glu Arg Leu 
                245                 250                 255     


Leu Val Ile Ser Leu Gly Thr Gly Ser Asp Lys Val Glu Gln Leu Tyr 
            260                 265                 270         


Asn Ala Lys Ser Ala Ala Lys Trp Gly Ile Ile Ser Trp Leu Phe Asp 
        275                 280                 285             


Asn Gly Asn Thr Pro Leu Leu Asp Ala Phe Asn Gln Ser Lys Ala Asp 
    290                 295                 300                 


Met Val Asp Phe His Asn Ser Val Ala Phe Gln Ala Tyr Gly Ser Leu 
305                 310                 315                 320 


Asp Asn Tyr Leu Arg Ile Gln Asp Asp Thr Leu Lys Gly Val Ser Ala 
                325                 330                 335     


Ser Val Asp Val Ala Thr Thr Glu Asn Leu Ala Asn Leu Val Thr Ile 
            340                 345                 350         


Gly Lys Ala Leu Leu Lys Lys Pro Val Ser Arg Ile Asn Phe Asp Thr 
        355                 360                 365             


Gly Arg Tyr Gln Pro Ile Pro Asn Gly Gly Thr Asn Glu Glu Ala Leu 
    370                 375                 380                 


Ile Arg Phe Ala Lys Leu Leu Thr Glu Glu Lys Ser Arg Arg Glu Gln 
385                 390                 395                 400 


Tyr Arg Thr Asn Arg Ser Asp 
                405         


<210>  25
<211>  14701
<212>  DNA
<213>  Beta vulgaris


<220>
<221>  misc_feature
<222>  (10783)..(10802)
<223>  n is a, c, g, or t

<400>  25
gagtgtggag tagttgcttt gtgcgagcgt gttttgtttg tgcgaacttc tgctgtcacg       60

ttttgtttgt ggaattgctg tcacgtttaa ttaccattca agtgtgatct ccttctagta      120

gattgtgatc ttgcttgtgg tatattggat ggtgtgagcc ccagacgtga ttttgttcgg      180

ttgatgcggt tgtgcatcgt ttctccaaaa ccctagttgg tttggtgcgg ttacgcatcg      240

ttattttgat gggttatggt ggcccatcca agctattgtg ttggtgcggt tgtacatagt      300

tgatgcccta agtgacgaat tgaatcatga aggttggttg gttgtccata attcaaaaag      360

ggtatttctt ggttgtccat aattcaaaaa aggtatttct tggttgtccc taatccaaaa      420

tggtgtgtat tggttgtccc taatcttaaa agctggttat tgaaggcgtt gatttcacgt      480

cttattggtc tcctagtaat aattctttta tatatttttt caattggagt gttggtgtta      540

ctatcctatg attgaggggg gagtattaga gttatggtaa gtatattata ttagagtatt      600

atgtttggag tattatattt attaggagtc ttgtattata tacctacgag ctttcatatg      660

cacgcgatgc gtgcgttaat ttcaaaattt gttattctat tagatactcc attcgtactt      720

tatttatgat taaacttaaa tgtgaaaaat tcaacagtta gatgaattta gatatattaa      780

ttcgctaatg taaaaaaata aaaaggttgt gagtgattgt taacattaat tttttgtgaa      840

tgaatattga acagaaagaa caaaaataat gagttggttt aactaaatta aatagtatca      900

aaagtagtga atgttcagac ttttcatgtg cacgttatgt atgccatctt ttttttataa      960

gttataaata ttttgtccat ttaatttttg attgaagtat ttgatgcata taccatttta     1020

aacttgtttt aaatggcaag ttggaatgag aattttttaa atgatttgct tttgtaattt     1080

tttatattct attgattaga tcattaaggg tattttggtc actttaaaag aggacaccac     1140

acctcctccc ttggcttata taatagagat ctgttaagtt gttagtacgt atatctagtt     1200

cagtaaggat ttctgatatt aggttgtata tatatattcc tcaaaagttt gataagtttc     1260

ccatattcta aaccttaaca ctttgagtga gtgaaatgtg gcagaagcta atttctttta     1320

tgtatattga tgatatacac agaatgcatg catgtgttta tagaagactt accattcaaa     1380

atcaacaata tacatggatt agaacaattt ttaaaatgta cttcaatttt ctctcactgg     1440

acttggacac gattggggag aagaaggtgg tggacaccgt gtaggaggag aagactgaag     1500

agaagacata caccatacct ttcaagaaga aggatagagt atgtgtgtat gtattagcta     1560

ggcagactgt tggagtctag aggttgagat gtggaataag aaggagaata atacgggtga     1620

gaggttttag ttaattgtca aatgttgcgc tctagcatat gtccttattg atagatgact     1680

gtcaaagttt ttgatcacta gctccttatt tcactactaa atactaatta cactaatcga     1740

ctgtacatac acccacacac atatatatac atatatgaga atgagggata acctagtggt     1800

taatgcttct ctcccggttc caggagtcct atgatcgatt ctcacccttg acaagttagg     1860

ttctccttta ttccccaagc ttatcacacg tacacacgtc gctgtcatag acatgtcatc     1920

tttaagttta agttaaaaat ttataataaa ttaattaagt gtatggcatg catgtatagt     1980

tgcttccagc aattaattcc tcctaaaatt aaatagttag cttaccagtc cacaatgtag     2040

gaataacaaa gaataaatgg gggaagtaga gtgaatatat tatataattc atataatagt     2100

ctcgcggcta ttaagaagac gatttttgct aagctggtta tggtgtgttg tttcttcttt     2160

tttttctctc ttgctcaggt aattaggttg gtgtttgaga aataatactg aaaatgacga     2220

acttacaaag ctctcctcca tccttcaatt actacaacag gaagaaattg gtcacaatcc     2280

ttagcatcga cggtggtggc attcgaggca ttattcctgc tgtcattctt gcttttcttg     2340

agcgacttct tcaggtacgt acataaaaca taatcatact acatatacat atctcctata     2400

tatccattca tctctcgcac tcatttgtac tcatttagcg tgtgaatcgt gcatatcaca     2460

tcgaacaatg ttattttgat atgaataatg ataattaggt tcctagtaga gttgggcatg     2520

atagccgggc tagtccaaca cgttccacca tgactttatc cggttatccc cacacgatac     2580

aattttgttc gaaagtgaaa gacactgtta tgggtcatat atagacatca tttttaaaat     2640

ttggcataaa tatgagaact atgtaatttc tatcttgtta tcattgatac cttgtaaaac     2700

tatgcaatcg tgcatgcacc taatgaataa taacaataaa caataaagta actaattaag     2760

tataatgagt agtgtacaat tgtacatgtg cacaataatt tcagatgcga aatgaaaata     2820

ttatttacta cgttcataat atgttaaaaa aagcactcct gagacataat gtgatgcata     2880

gttaccccgc aaaaaacaat taattaatat gatgtcaggt taattggtta taattatgta     2940

ggagttggat ggagaggaag taagactagc agattatttt gatgtgatag caggaacaag     3000

cacgggaggg ttgattacag ccatgttaac tgcaccagat gactataatc gtcctctata     3060

tgctgcgaaa gatatcacaa cattttactt gaagcatggt cctcaaatat ttcctcagga     3120

aaggtaaaaa atttaaatgt cagaatattc cataccacaa tataattttt aataagtagc     3180

ctataaatgg tggtcacacg ctaagcatga caattttatt aaaagatcaa tcgaattttt     3240

attaaatctt ataggattaa aaattagaat tcattgggtg gaaaaaaaaa tgaaaaaggt     3300

tgtgattttt cgtccatcgt taaatcgaaa caaaaagaaa agtcaaagta aaggcttatt     3360

atttctcatt tataaatatc caaattttag tacccaatac ccataaatag ttcgaaccat     3420

atagacacaa taatcaattt tcgtgtgaat ggtttgtagg gaaaccctat cctttcttat     3480

ccataatggt gtataaataa attttaccca caaaaatata caagtttctt catatactat     3540

atcatgaaaa taactattat gacatatatc tacttagtta cccgtcgagt aggtttacta     3600

aacttatcca tacatcaaat atgtcaaaaa gaatccagtt aattgtttga ggatgtgagt     3660

catgtgattg gtgagctatc aattgttcat cactttctaa attcaaactc gaggaagtaa     3720

tcaattggtc ttggcatagt ggttgtccac tttgtccctt aatcaattat cgaggattcg     3780

attctcaatt tttgggaatg gaaagatatt tttgaccagt cctttaccct taagtgtagc     3840

actcgaagcg agaggattaa tcattgctgt cggcggtgaa tacttagatt gtttttaaaa     3900

aaaacttgag ggagtgatac tagcagatca tgtatcctca tgtttgtcaa tgaaggtgtg     3960

caaagtatat gctagtatgc tactaccgcc catgaccctg aaattcaatt tttcattgtt     4020

tgttttagtt gcattgtcct atttggctat ttcaatactt tagtaacttt aaaaaaaaaa     4080

tacttcctcc atctcttttt actcgctaca ctttttatta tgggaagttt cacattactt     4140

gctacacttc ttttttgggc aataaactat ctctatctct ctcctcccac atgagtcccg     4200

tgttgagatc ctctagagtt ccaaaaaaac tctacacggt agagttcgtt aaatatcagt     4260

cgttgattaa gaaatcaaag gcttgtattt tgttttccaa aaatcccccc tattttcccc     4320

cgaatatcct gccaatatct tttccttttc caccaatcat gagcctttga ttttatgata     4380

tatggtcagg attcaactct actttgtaga gttttcataa aactctagag gatccggacc     4440

ccatgagtcc cttttctttt tattctctac ttctctcttc tccaaaaatt gcctaggagt     4500

ccgtgaaatg agaagtgtag cgagtaaaaa gaaacggatg aagtatattg ttaatatttt     4560

ttaaaatata tatattttat tgtaggatta atcttaataa ttactaaggg atttccaaca     4620

tgtttgatgt cgcctgtatt ttgctgacca agtttagaaa aattgatagg ttgtttctaa     4680

catctttttg ccggccacca agctggagat gtagacatat agaagtagtt tatgattttc     4740

ccactttgaa ctggttatac atacattaat tagttattac aagtcttttt tagatgatta     4800

ctccatcgat tccaccattg ttcatgtcat cttgtcatat acagcgactt tcgtaactat     4860

tgtgtattat tattatatac tccctcttta ccaatctaga tgcaattatt ttccttttat     4920

ggtttctccg gacacacttt ttacatctat gattatatat tagtagaatt tagttataga     4980

tgatatattg taaatttata cattattgaa acaaatctaa tatgatattt atatgataat     5040

tttatttgct tatatcagta aaaaaatatg cttagttaac attgatgatc tttaacccta     5100

gaaaaacaaa tgttgcaagt tatatggaat taacaaagga ggttatattt ttcttggaaa     5160

agaaccatag ttgtctcttt ccttgaagta caattatgat gtcagtgggt aatggaatct     5220

acccggatcc aaatcatttt ttagcgattt gtatcctcaa attttggacc caacggatat     5280

ggatcatgtc ttaaatcatt acatggagtc gtatccattt acccattttt taaaaattat     5340

tttaaaatat ataattaaaa gtgaaaaact taaaggattt tttaactgga ataatacaaa     5400

ctttgggccg ttctctcgca ataatacaaa cttttgatta ttttttaata atacaaactt     5460

taggccaaaa ttcctccagt aggttaatac cggttggtaa cttgttgtag gaggtaacca     5520

agctgacgtg gcacctttag gacacgtgtc tttatttgat tggctgtttt tttaaattta     5580

gggaaaaaaa aaaccaaaat ttttcttttt tttttctttc ttctcctgct ccccccttca     5640

ttctctttct cactactcca ccccaccgac ccccacccct ggcccctgcc cccagcccct     5700

ccctgcctgc acaccgcatc cctccgacga ccaacccccc atctctggcc cttcccccac     5760

cccgaaacca taccgcaccc ctccgacaac cacccctggc ccctccccca cccgaaaacc     5820

acaccgcacc tcttcgacaa ccaaccccca tttctggccc ctcccccacc ccgaaaccac     5880

actgcacccc tccaacggcc atcacctgac gccattctcg atcgctcgaa cttagatcct     5940

ttcccgcaca acaacaagtc gtcgtcgtca ccgcgcgaaa tcaatacgat attcgaaaat     6000

ggcggtcatt ttattatcga cgaattggag gaggaggaag agacgtcgtt gttgacgatt     6060

tcgcatagtt acgcgatctg gggaagtggt ttcgtgtggt ggtgagtttg aggtggtttc     6120

gtggtggggg agtttgaggc ggtttcatgg ttggggacga ggtggtgatg gtggtttcat     6180

ccgtgggggt ggagctgtgg gagggggtgg aactgtggag gtgagggtgg ggggaggggg     6240

tgggggtgga gctgtggagg tgggggaagg gggtgggagt ggagccgtgg aggtggagga     6300

gggggtggga gtgggctttc acattttcag ttggttggct aggggaagga ggaagagatt     6360

ttggagttta aaaaaatcga cctattctac atgttaatgg tcaacttgac ctactggagg     6420

aattttggcc taaagtttgt attattaaaa aataatcaaa agtttgtatt attgcgagag     6480

aacggcccaa agtttgtatt attcgagtta aaaaatccaa acttaaaacc attttatgat     6540

tttatctttc aaattactta tatgtttatt gtgatgaata tttatgttgt cattattatt     6600

ctttgtaatg tacaaatatg attttgcaat gaacaacata ctatcatata cttcctccat     6660

ttctaaataa gtgcaacatt tcttgttttc acatttgcca atgcattatt ttaaccagta     6720

atatcttcaa ttatcaataa gtaaaaatta taaaaagttg atattaaaaa aattcacatt     6780

tagatgaatc taacaagatc ccacatgact atgtttttcc taaaatgcaa atcactaaag     6840

ctaattgatg tttaaactgt gaatagtaaa cactatgcaa atgttacact tatttagaaa     6900

cggaggaagt aataaataac ccaattgaat agttatataa ttatcaaaaa ataagtaatt     6960

acaaaagatg gttaattcat aatgtagtat gtacccaagt gtagaaatat aaccaaaata     7020

atataatata tttctaatta aacactattg agttcattaa gaagatattc ttgcttttga     7080

gcttccttcc atttccgatt gatttgaata gtgagatctc gacaccataa cgtatcttag     7140

ttgtttgaac tagtcgaatt gaactgatac aagaaaggaa atagcagtag ttaaaaaaaa     7200

ttattcaagt ttataataca atgaacataa tttttcaaac atacaattaa cacattctaa     7260

taatacaata aacatgctcc cgcacatagt cccgactatt aggctgtgca aacaacccaa     7320

tagcacagga ccccgaattt cttagggtcc ccaaaatctg gaaacaaaaa aatatagttg     7380

attacggcgc taaaacaatt caactacata tattagcaga gttgttatat gagattaact     7440

ctacaacaag aatacgcaag ttcgactcat gttccgggaa tatctttaca tactttttgc     7500

aagtgtttat gatattttct tctagtttga gcatttcact catctcccca ccacttcata     7560

cttactttct aactattttc cttacttttt tttcaatact tttttttgat aaattgtttt     7620

tctcaattct ttagggtact tttttgataa attcttttat caattcctta gggtacttat     7680

tttttttgga agtgtatatt tgtccaataa gaattgaata atttcctgcc acaagtttac     7740

tagtttaccg gtttagtcac ctcatcatat atataaattt tttttttttt gttgtacctt     7800

ttagagtgag atagaaaaat tattatcgtt atctttataa gtagaacatc agtcataatg     7860

tggttttata ttgagttgtt acacacaatt tcaaatgagt ttgaggtttt agtttttcga     7920

tttacaagat aaatttatat aattgtaata ttataaaaga ttttttttat catttgagtt     7980

gcaaaaaaat gagattaggg gtccaatcct aaaattggaa caagacctcc aaaatgttta     8040

agacggccct gcccgcacac aatgagcatg tgattaaaat gagcatgtta gtaatacaat     8100

gaacatcaac ctaaaaatgt gcaccttgta tttgtaaatg gtacaataaa tatcacataa     8160

tcatacaaag aacatttgaa caaatatcac acatttatag acaaatatat atgataatca     8220

acccatcatt aattcatatc atttcattga atatattaca aacacataac gaattatgac     8280

tgaaatgcaa taaacatctg accaacatgt gtttcagtga acatattctg attggacaat     8340

gaacatatgc cataattcat gttattaatg caattaatat gtgtcaaaga cataataaat     8400

atatgaagaa ctgtacataa catctgaaca aacacataat aaacatatgg ctaaatgtaa     8460

taaacatatg accaacacaa attcaattag tacattacaa taatacaacg aacatatatt     8520

tacagtacaa cattacctgt ttttttctag atgtcgaact cgttcatctc ctttattcat     8580

tgggctctct catttgttca ttgggacatc aattgcaagg ttgttttcat ttttcacaaa     8640

tatcaagatc ttcactagaa aagatggaaa aaaaaataag aaatgaaagt gcaatatgta     8700

aaaaaaaata ttcttttttc ttatcaaatg catagatcta ataacttatg ttctttgcaa     8760

ctcgttgtat tcatcttcat gtacgaaaat tgtttaaaaa aatgaagaat ttaaaaaggt     8820

tgatgagaaa ggttagagag aagttaaaga gatgttggag gatttgggga gaagagataa     8880

aaagtctttg aaaatttctg gggaaaaatg aaagtaatga attcaaattt ttttatgttt     8940

tttggtgttc attggagatc aacaaatgtt catttcgtgc atttttggtt taatgaatac     9000

gcgttttatg ttttgttgac atccgttttg acccatgcac accttcctca acatacatcc     9060

gttcgcacgg ttgatctact gatcattcgc ttgaaaaggg aataaggtca tgacagcgtt     9120

aagaatgaaa caagaaagat cgtatatttg ttggtaactt ggtactacaa ttagggttgt     9180

tcaccggtcc aggatcggat cgaacctgac ctgcctggac cgttctttat gggattcttc     9240

ggaccaggtg tggatcggaa aaatcaggtc tggtcttcag tccggacctg ttgggccgga     9300

aaaattaact tgtatctctg attcttcgga cctgttacga cccaccatca ataaatatca     9360

gaattccata gatttatcag tttacagagg accaactaca acaagcaaac aaaaaaacaa     9420

ttctagtact tcctccgttt ttttttttac ttgcaacact tttttttcat caactccaat     9480

gtaaaatttg ggtatgagat aggcatacag gaaaaaaaga gagatcccca tgtgattgga     9540

gagagataga gagaaaggaa aagaaaaagt agaaagtgtt gcaagtaatt aattattttc     9600

tttttaggaa agtgttgcaa gtaaaaagac ggaggaagta tttagttttt attacaaatt     9660

gtttgggggg ggggggcagg agacatggag tcacactaga ctaggacact caaagaggca     9720

ccaatgctac tcactaagtg attaaagtta aagatagaaa agggtgaaaa agactcgcga     9780

actgaaaaac aaacctgatc ggtggtgggc ggtagctgcg gtcgcctgag cggcgtcgtg     9840

gcgtcggtgc gactggtttg gcgaggcgtg accgcgtgag gactgaggtg tttgagttct     9900

cttagggttt cttttagaat gactaaatga gagagggaga agtaggaata aagggctggc     9960

atttttttaa aaaaaaaaaa tactaaaaag cttccgagtt ttcggtctgg accggatttt    10020

ttcgagtttg aaccagaccg aaccggaaac ccggaataga cacattccac ggaccgggga    10080

ccggattgga atcgccggtc cggttcgatc cgatccaaaa tccggtccga ttagatccga    10140

cccacctatg tgaacagccc tagctacaat actacatgac tacctgcctt attaactctc    10200

ccttttatta gatttttcgt tagctaataa ctcccttagg atattgaatc tactttgttg    10260

gttataactc ttataagtta ctattatagt aggagaacaa ctattattat gtgcaaaatt    10320

acacagaatt tgtttttctt ccatttttag gggaccactt gctcagctga ttagcttcat    10380

caaagcaatg acagggccaa aatatgatgg caagtattta cgccatatac taagggaaaa    10440

attgaaagag acaaggttgc atcacacact aacaaatgtt gtcattccaa cttttgacat    10500

taagaccttc caaccagtta tattctcttc ctacaaggta aataaattat aatcgtgtgc    10560

tcatctccaa taattttacc ttaaatgaat tttcatcaac taatcatttg ctttgctgat    10620

gaccatcctt gatttgaatg aagagaatat atatatatat atatatatat atatatatat    10680

atatatatat atatatatat attatatata ctatatatat agtacgtagt acgtacgtac    10740

gttacgtacg ttacgtagta cgtacgggta cttaacgtcg tannnnnnnn nnnnnnnnnn    10800

nnagtatagt agtatatata tatatatata tatatatata tatatatata tatatatata    10860

tatatatata tatatatata tatatatata tatataggat gaggaactag ctcatgtggt    10920

agagcactcc cctctgatcc ttgaggtcag gggttcgatc cttaccccca acattatgtt    10980

ggggcatttc cttagcatgc tttgcatgct ttaccagcca atgtgctggt agttcctaca    11040

aggctagtag aatgaatttt acgtgttata actcatatga ataagtgatg taactccaaa    11100

aaaaaaaata ttagaaaaaa gaaattcaag aaaaaaaaaa ttgggatcaa aattttttaa    11160

ggggtataac tctaataaag tattgtaact ctaatccaat catgtggtcg aaattcattc    11220

tcatttctca tttaaagagc attctcatat gatattactc ctatatatat tcgtgcttgt    11280

ttttttcttt attagatgat cctagcttga tatcgacgca aactaagaac ttcaaaagga    11340

aataatgaaa ttactatttt aatgttttct ttttgtttta ttccatatat atgtaaacac    11400

agtattatcg atctgatagg cataaatgtt atacttaatg tgattatcca aatagtttaa    11460

ttgagagttt tactcttatc aagttatcat gtcgtctagt gttaataatt ctttagtttt    11520

atgtctattt tgtcgtagct atagtccgac tggtcaagta cttgatcaaa cagtttaagc    11580

ggtgaaccta ttgcatgact tttacctagg caactagtat atgtctggaa aagtgaatga    11640

cgatctctca ttttcatagt gaaatttaca atattctcta agcttttatg atattaatga    11700

taggtgatat atgatgcgag gttattgaag tgtgtggatt ttcctagtgc cctttagtta    11760

cattgttaat ttgttagggt tgattcaagt agatttacag acctatatga gggcataact    11820

aagagaccat attttattga tggaagaaaa gtagagaggg tcactttagc agctactcct    11880

actacgagta gctaagtatt acggatttaa aaaatattcc tcccaatgat agaaattaag    11940

gtcgaaataa aacaacattt taagataaca catatttata aaaacaaaac tagtgaaaaa    12000

taacctagac aaattcgaat cttatttgca tcgacaaact ccaacatttt catcattatt    12060

ttcaaaaaca tatcaaaaca ttgaaaatgc cagatcttct gtccgccccc tccctttgac    12120

caaaatatga atttatacgt aagtagtttt accttcgtaa ttttacattt gtgattttgt    12180

agacttcaat taacattttc atttgtgttc aattaaactt tgcgacttag tcaacggatt    12240

aattacatcc ttctttatgc ataaaaaagt ccatttatca atcaacccaa gaagactcac    12300

aatgttttta tcatttaatt tttgcaaatt ctaagttatc atttaatttt tacagattct    12360

aagttatcct gatctggatg cgaaattatc agatatatgt ataggcacat cggccgctcc    12420

taccattctg ccttctttct atttccaaaa tgcatttgaa aacgaaaaga caagggaatt    12480

caacctcatt gatggtggaa ttgtgagtac aaatccggta agtcaagctc aatcaatcat    12540

attattattc ttatgcttag aggatgcaca atagcatggt ttcacttggg gcaaaggtga    12600

accaatgggt gaggttggtc ttaccattgg ggtgagtcaa ggcaaacatg gcttaatgag    12660

gttgggataa tttgctcccc ctctctccca acccatgcat cggaccaaga gcccaagcta    12720

gtaacaaaag gcttgtgtac tttcacagag aatgagcttc atgtctttga gtgtagagct    12780

tttttccttc ccaaacgtcc tactcttaga attgttattt acataaccta ctttaattat    12840

ttttttttat taatttataa aatagttgtt atgttattac atagtaagtt gtcgtatcag    12900

agttcttttc acgaaaactc aaagctgaaa cataaaaaca gacgagttta ttcatcatac    12960

cacctagata taccacaatg ttagtacatt ttgttaattt ggaactatcc aaatgaacaa    13020

taagcagaaa aatagtcttg agtgtaatgt ctacaatgtt cacaatacat tcaagtgtat    13080

gttgaatttt ttgtataaga gcctaaaaaa cttttatcca acaatacttt aaaccagcag    13140

atagaatatt atttgatgta tcaataattt cattctatct ttttgttttg tcgtctgggc    13200

agacttatct ggcaataaat gaagttacca aacaaatgat gaaggagaat ccagattacg    13260

ggaccatcca taataagttg ctggttttat cgataggaac aggatcagga aaaattgagc    13320

agaaatacaa tgctaaaact gcaggaaagt gggggcttgt ctcttggctt tttcaaaatg    13380

gatcatcacc aattataagt gctttttatg aagccggggc tgacttagtt gattaccaaa    13440

ataatatcct cttccaatcg tttcgttcag aggacaagta ccttcgtgtg caagtaagtc    13500

gcatgatacc cttcaaattt aactttaatg tagtaatccg tttacgatta atcggcacat    13560

attgttggaa ataaaacagg atgacagctt aacaggaaca actgcttcaa cagatgtagc    13620

aacagagaag aacttggaaa atctagtgaa aattggtcaa gaattgctta acaaaccagc    13680

cagcagggtg gacccagaaa ctgggcatct gaaagcaatt ccacaccttg gtaccaatgc    13740

agatgctctt cgaaggttgg ttcataaatt ttataatatt gccaatataa attgcacatt    13800

attagttatt actactagat agattaacaa gtcattaaca aataggttcg caaaacaact    13860

ctcggatgaa agaaaatacc gcaggggcaa agactccaat cagatgcaag agtacagctg    13920

agctgacgat gtgcaggcat ttctgagtac tgagctgtct ttgattgtat actatatgat    13980

aaatgtgtgc aatgtaattg acctcaaatc ttctatctca taatttttgt actattctgt    14040

tggttgatta taaatttgta acgttctaaa gattatctta aaaggtacta gaccgtagta    14100

caatacctgc taataatata tagtagcata cattcttttt ttggctccct tccgggaatc    14160

cgtggtaaga tgaatttcct ttagactgtc ctcacacagg gccactcacc cagaagacca    14220

acaacccccc tccccccaac tatattgaaa tttttgcttg caccaagatt tgaaccttgg    14280

cttcccatat gtgagacacg aaaccatacc attggaccca agggtccttg gtatataaag    14340

tagcatacat gtgacacaca aagagagaca gtagatgttg cttcaggtaa tgcagaaacc    14400

ccctatatga tcaacatgat caacagaact atgtatatct atacttcttt ccctagataa    14460

gcaagaattt atggaggcta actatccttg tttccctgta atgcttatac aagaagatgc    14520

aaaatagaaa gctcagagcc agatactctc ttaatagacc caggagtaac ctgaacttgc    14580

taccatattc aacacattta agccagaagg taaaacacag aagttcgaaa gtaaagacag    14640

caagttcaca aagctaggga tctttttgaa gtgcaaccat cggacattat gggattgcta    14700

a                                                                    14701


<210>  26
<211>  1215
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of Beta vulgaris phospholipase 2

<400>  26
atgacgaact tacaaagctc tcctccatcc ttcaattact acaacaggaa gaaattggtc       60

acaatcctta gcatcgacgg tggtggcatt cgaggcatta ttcctgctgt cattcttgct      120

tttcttgagc gacttcttca ggagttggat ggagaggaag taagactagc agattatttt      180

gatgtgatag caggaacaag cacgggaggg ttgattacag ccatgttaac tgcaccagat      240

gactataatc gtcctctata tgctgcgaaa gatatcacaa cattttactt gaagcatggt      300

cctcaaatat ttcctcagga aaggggacca cttgctcagc tgattagctt catcaaagca      360

atgacagggc caaaatatga tggcaagtat ttacgccata tactaaggga aaaattgaaa      420

gagacaaggt tgcatcacac actaacaaat gttgtcattc caacttttga cattaagacc      480

ttccaaccag ttatattctc ttcctacaag attctaagtt atcctgatct ggatgcgaaa      540

ttatcagata tatgtatagg cacatcggcc gctcctacca ttctgccttc tttctatttc      600

caaaatgcat ttgaaaacga aaagacaagg gaattcaacc tcattgatgg tggaattgtg      660

agtacaaatc cgacttatct ggcaataaat gaagttacca aacaaatgat gaaggagaat      720

ccagattacg ggaccatcca taataagttg ctggttttat cgataggaac aggatcagga      780

aaaattgagc agaaatacaa tgctaaaact gcaggaaagt gggggcttgt ctcttggctt      840

tttcaaaatg gatcatcacc aattataagt gctttttatg aagccggggc tgacttagtt      900

gattaccaaa ataatatcct cttccaatcg tttcgttcag aggacaagta ccttcgtgtg      960

caagatgaca gcttaacagg aacaactgct tcaacagatg tagcaacaga gaagaacttg     1020

gaaaatctag tgaaaattgg tcaagaattg cttaacaaac cagccagcag ggtggaccca     1080

gaaactgggc atctgaaagc aattccacac cttggtacca atgcagatgc tcttcgaagg     1140

ttcgcaaaac aactctcgga tgaaagaaaa taccgcaggg gcaaagactc caatcagatg     1200

caagagtaca gctga                                                      1215


<210>  27
<211>  404
<212>  PRT
<213>  Beta vulgaris

<400>  27

Met Thr Asn Leu Gln Ser Ser Pro Pro Ser Phe Asn Tyr Tyr Asn Arg 
1               5                   10                  15      


Lys Lys Leu Val Thr Ile Leu Ser Ile Asp Gly Gly Gly Ile Arg Gly 
            20                  25                  30          


Ile Ile Pro Ala Val Ile Leu Ala Phe Leu Glu Arg Leu Leu Gln Glu 
        35                  40                  45              


Leu Asp Gly Glu Glu Val Arg Leu Ala Asp Tyr Phe Asp Val Ile Ala 
    50                  55                  60                  


Gly Thr Ser Thr Gly Gly Leu Ile Thr Ala Met Leu Thr Ala Pro Asp 
65                  70                  75                  80  


Asp Tyr Asn Arg Pro Leu Tyr Ala Ala Lys Asp Ile Thr Thr Phe Tyr 
                85                  90                  95      


Leu Lys His Gly Pro Gln Ile Phe Pro Gln Glu Arg Gly Pro Leu Ala 
            100                 105                 110         


Gln Leu Ile Ser Phe Ile Lys Ala Met Thr Gly Pro Lys Tyr Asp Gly 
        115                 120                 125             


Lys Tyr Leu Arg His Ile Leu Arg Glu Lys Leu Lys Glu Thr Arg Leu 
    130                 135                 140                 


His His Thr Leu Thr Asn Val Val Ile Pro Thr Phe Asp Ile Lys Thr 
145                 150                 155                 160 


Phe Gln Pro Val Ile Phe Ser Ser Tyr Lys Ile Leu Ser Tyr Pro Asp 
                165                 170                 175     


Leu Asp Ala Lys Leu Ser Asp Ile Cys Ile Gly Thr Ser Ala Ala Pro 
            180                 185                 190         


Thr Ile Leu Pro Ser Phe Tyr Phe Gln Asn Ala Phe Glu Asn Glu Lys 
        195                 200                 205             


Thr Arg Glu Phe Asn Leu Ile Asp Gly Gly Ile Val Ser Thr Asn Pro 
    210                 215                 220                 


Thr Tyr Leu Ala Ile Asn Glu Val Thr Lys Gln Met Met Lys Glu Asn 
225                 230                 235                 240 


Pro Asp Tyr Gly Thr Ile His Asn Lys Leu Leu Val Leu Ser Ile Gly 
                245                 250                 255     


Thr Gly Ser Gly Lys Ile Glu Gln Lys Tyr Asn Ala Lys Thr Ala Gly 
            260                 265                 270         


Lys Trp Gly Leu Val Ser Trp Leu Phe Gln Asn Gly Ser Ser Pro Ile 
        275                 280                 285             


Ile Ser Ala Phe Tyr Glu Ala Gly Ala Asp Leu Val Asp Tyr Gln Asn 
    290                 295                 300                 


Asn Ile Leu Phe Gln Ser Phe Arg Ser Glu Asp Lys Tyr Leu Arg Val 
305                 310                 315                 320 


Gln Asp Asp Ser Leu Thr Gly Thr Thr Ala Ser Thr Asp Val Ala Thr 
                325                 330                 335     


Glu Lys Asn Leu Glu Asn Leu Val Lys Ile Gly Gln Glu Leu Leu Asn 
            340                 345                 350         


Lys Pro Ala Ser Arg Val Asp Pro Glu Thr Gly His Leu Lys Ala Ile 
        355                 360                 365             


Pro His Leu Gly Thr Asn Ala Asp Ala Leu Arg Arg Phe Ala Lys Gln 
    370                 375                 380                 


Leu Ser Asp Glu Arg Lys Tyr Arg Arg Gly Lys Asp Ser Asn Gln Met 
385                 390                 395                 400 


Gln Glu Tyr Ser 
                


<210>  28
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R59Q mutant - primer AlleleX

<400>  28
gaaggtgacc aagttcatgc tccgtccagc tcctgcagct                             40


<210>  29
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R59Q mutant - primer alleleY

<400>  29
gaaggtcgga gtcaacggat tcgtccagct cctgcagcc                              39


<210>  30
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R59Q mutant - primer common

<400>  30
tccccggaac catccttgcc tt                                                22


<210>  31
<211>  201
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  partial sequence indicating detectable single nucleotide 
       polymorphism that causes amino acid substitution R59Q in Sorghum 
       bicolor phospholipase

<400>  31
gagcccgtcg tgctggggca gagggtgacg gtgctgacgg tggacggcgg cggcatccgt       60

ggtctcatcc ccggaaccat ccttgccttc ctcgaggccc rgctgcagga gctggacggg      120

ccggaggtta ggctcgcgga ctacttcgac tacatcgccg ggacgagcac cggcgggctc      180

atcaccgcca tgctcaccgc g                                                201


<210>  32
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V162I mutant - primer AlleleX

<400>  32
gaaggtgacc aagttcatgc tgagcgacac gctcaccaac a                           41


<210>  33
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V162I mutant - primer AlleleY

<400>  33
gaaggtcgga gtcaacggat tgagcgacac gctcaccaac g                           41


<210>  34
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V162I mutant - primer common

<400>  34
ctgcagcagc ctgacgtcga a                                                 21


<210>  35
<211>  201
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  partial sequence indicating detectable single nucleotide 
       polymorphism that causes amino acid substitution V162I in Sorghum
       bicolor phospholipase

<400>  35
catgtccgcg ctgaggaagc caaggtacaa cggcaagtgc ctccgtaacc tgatcatgag       60

catgctcggc gagacgaggg tgagcgacac gctcaccaac rtcatcatcc ctaccttcga      120

cgtcaggctg ctgcagccca tcatcttctc cacctacgac gtacgtacgc cgccggccgc      180

cgtcgtcatg aataatcaat c                                                201


<210>  36
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S291L mutant - primer AlleleX

<400>  36
gaaggtgacc aagttcatgc tggcagatgc cccaccgcg                              39


<210>  37
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S291L mutant - primer AlleleY

<400>  37
gaaggtcgga gtcaacggat tggcagatgc cccaccgca                              39


<210>  38
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S291L mutant - primer common

<400>  38
gcctgtacac ggcgcggca                                                    19


<210>  39
<211>  201
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  partial sequence indicating detectable single nucleotide 
       polymorphism that causes amino acid substitution S291L in Sorghum
       bicolor phospholipase

<400>  39
ctgtacccag tgaagccgtg gaactgccgc aagttcctgg tgctgtccat cgggacgggg       60

tcgacgtcgg agcagggcct gtacacggcg cggcagtgct ygcggtgggg catctgccgg      120

tggatccgga acaacggcat ggcccccatc atcgacatct tcatggcggc gagctcggac      180

ctggtggaca tccacgtcgc c                                                201


<210>  40
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Q372stop mutant - primer AlleleX

<400>  40
gaaggtgacc aagttcatgc tgaccctgga cacccgctg                              39


<210>  41
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Q372stop mutant - primer AlleleY

<400>  41
gaaggtcgga gtcaacggat tgttgaccct ggacacccgc ta                          42


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Q372stop mutant - primer common

<400>  42
gtcgggatcg gggagcggat                                                   20


<210>  43
<211>  197
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  partial sequence indicating detectable single nucleotide 
       polymorphism that causes amino acid substitution Q372stop in 
       Sorghum bicolor phospholipase

<400>  43
gcatccagga caactcgctg cacggcgccg cggccaccgt ggacgcggcg acgccggaga       60

acatgcggac gctcgtcggg atcggggagc ggatgctggc gyagcgggtg tccagggtca      120

acgtggagac agggaggtac gaaccggtgc ctggggaagg aagcaacgct gatgcgctcg      180

ctgggatcgc aaggcag                                                     197


<210>  44
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer

<400>  44
ggcccccatc atcgacatc                                                    19


<210>  45
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer

<400>  45
aatcaggcaa caaattcact tctc                                              24


