                         SEQUENCE LISTING

<110>  Sanford Health
       Vermeer, Paola
 
<120>  Inhibitors of Ephrin B1 for Tumor Treatment

<130>  16-1636-PCT

<150>  62/462825
<151>  2017-02-23

<150>  62/548264
<151>  2017-08-21

<150>  62/616376
<151>  2018-01-11

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  345
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  1

Met Ala Arg Pro Gly Gln Arg Trp Leu Ser Lys Trp Leu Val Ala Met 
1               5                   10                  15      


Val Val Leu Thr Leu Cys Arg Leu Ala Thr Pro Leu Ala Lys Asn Leu 
            20                  25                  30          


Glu Pro Val Ser Trp Ser Ser Leu Asn Pro Lys Phe Leu Ser Gly Lys 
        35                  40                  45              


Gly Leu Val Ile Tyr Pro Lys Ile Gly Asp Lys Leu Asp Ile Ile Cys 
    50                  55                  60                  


Pro Arg Ala Glu Ala Gly Arg Pro Tyr Glu Tyr Tyr Lys Leu Tyr Leu 
65                  70                  75                  80  


Val Arg Pro Glu Gln Ala Ala Ala Cys Ser Thr Val Leu Asp Pro Asn 
                85                  90                  95      


Val Leu Val Thr Cys Asn Lys Pro His Gln Glu Ile Arg Phe Thr Ile 
            100                 105                 110         


Lys Phe Gln Glu Phe Ser Pro Asn Tyr Met Gly Leu Glu Phe Lys Lys 
        115                 120                 125             


Tyr His Asp Tyr Tyr Ile Thr Ser Thr Ser Asn Gly Ser Leu Glu Gly 
    130                 135                 140                 


Leu Glu Asn Arg Glu Gly Gly Val Cys Arg Thr Arg Thr Met Lys Ile 
145                 150                 155                 160 


Val Met Lys Val Gly Gln Asp Pro Asn Ala Val Thr Pro Glu Gln Leu 
                165                 170                 175     


Thr Thr Ser Arg Pro Ser Lys Glu Ser Asp Asn Thr Val Lys Thr Ala 
            180                 185                 190         


Thr Gln Ala Pro Gly Arg Gly Ser Gln Gly Asp Ser Asp Gly Lys His 
        195                 200                 205             


Glu Thr Val Asn Gln Glu Glu Lys Ser Gly Pro Gly Ala Gly Gly Gly 
    210                 215                 220                 


Gly Ser Gly Asp Ser Asp Ser Phe Phe Asn Ser Lys Val Ala Leu Phe 
225                 230                 235                 240 


Ala Ala Val Gly Ala Gly Cys Val Ile Phe Leu Leu Ile Ile Ile Phe 
                245                 250                 255     


Leu Thr Val Leu Leu Leu Lys Leu Arg Lys Arg His Arg Lys His Thr 
            260                 265                 270         


Gln Gln Arg Ala Ala Ala Leu Ser Leu Ser Thr Leu Ala Ser Pro Lys 
        275                 280                 285             


Gly Gly Ser Gly Thr Ala Gly Thr Glu Pro Ser Asp Ile Ile Ile Pro 
    290                 295                 300                 


Leu Arg Thr Thr Glu Asn Asn Tyr Cys Pro His Tyr Glu Lys Val Ser 
305                 310                 315                 320 


Gly Asp Tyr Gly His Pro Val Tyr Ile Val Gln Glu Met Pro Pro Gln 
                325                 330                 335     


Ser Pro Ala Asn Ile Tyr Tyr Lys Val 
            340                 345 


<210>  2
<211>  344
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  2

Met Ala Arg Pro Gly Gln Arg Trp Leu Gly Lys Trp Leu Val Ala Met 
1               5                   10                  15      


Val Val Trp Ala Leu Cys Arg Leu Ala Thr Pro Leu Ala Lys Asn Leu 
            20                  25                  30          


Glu Pro Val Ser Trp Ser Ser Leu Asn Pro Lys Phe Leu Ser Gly Lys 
        35                  40                  45              


Gly Leu Val Ile Tyr Pro Lys Ile Gly Asp Lys Leu Asp Ile Ile Cys 
    50                  55                  60                  


Pro Arg Ala Glu Arg Pro Tyr Glu Tyr Tyr Lys Leu Tyr Leu Val Arg 
65                  70                  75                  80  


Pro Glu Gln Ala Ala Ala Cys Ser Thr Val Leu Asp Pro Asn Val Leu 
                85                  90                  95      


Val Thr Cys Asn Arg Pro Glu Gln Glu Ile Arg Phe Thr Ile Lys Phe 
            100                 105                 110         


Gln Glu Phe Ser Pro Asn Tyr Met Gly Leu Glu Phe Lys Lys His His 
        115                 120                 125             


Asp Tyr Tyr Ile Thr Ser Thr Ser Asn Gly Ser Leu Glu Gly Leu Glu 
    130                 135                 140                 


Asn Arg Glu Gly Gly Val Cys Arg Thr Arg Thr Met Lys Ile Ile Met 
145                 150                 155                 160 


Lys Val Gly Gln Asp Pro Asn Ala Val Thr Pro Glu Gln Leu Thr Thr 
                165                 170                 175     


Ser Arg Pro Ser Lys Glu Ala Asp Asn Thr Val Lys Met Ala Thr Gln 
            180                 185                 190         


Ala Pro Gly Ser Arg Gly Ser Leu Gly Asp Ser Asp Gly Lys His Glu 
        195                 200                 205             


Thr Val Asn Gln Glu Glu Lys Ser Gly Pro Gly Ala Ser Gly Gly Ser 
    210                 215                 220                 


Ser Gly Asp Pro Asp Gly Phe Phe Asn Ser Lys Val Ala Leu Phe Ala 
225                 230                 235                 240 


Ala Val Gly Ala Gly Cys Val Ile Phe Leu Leu Ile Ile Ile Phe Leu 
                245                 250                 255     


Thr Val Leu Leu Leu Lys Leu Arg Lys Arg His Arg Lys His Thr Gln 
            260                 265                 270         


Gln Arg Ala Ala Ala Leu Ser Leu Ser Thr Leu Ala Ser Pro Lys Gly 
        275                 280                 285             


Gly Ser Gly Thr Ala Gly Thr Glu Pro Ser Asp Ile Ile Ile Pro Leu 
    290                 295                 300                 


Arg Thr Thr Glu Asn Asn Tyr Cys Pro His Tyr Glu Lys Val Ser Gly 
305                 310                 315                 320 


Asp Tyr Gly His Pro Val Tyr Ile Val Gln Glu Met Pro Pro Gln Ser 
                325                 330                 335     


Pro Ala Asn Ile Tyr Tyr Lys Val 
            340                 


<210>  3
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  3

Met Ala Arg Pro Gly Gln Arg Trp Leu Ser Lys Trp Leu Val Ala Met 
1               5                   10                  15      


Val Val Leu Thr Leu Cys Arg Leu Ala Thr Pro Leu Ala Lys Asn Leu 
            20                  25                  30          


Glu Pro Val Ser Trp Ser Ser Leu Asn Pro Lys Phe Leu Ser Gly Lys 
        35                  40                  45              


Gly Leu Val Ile Tyr Pro Lys Ile Gly Asp Lys Leu Asp Ile Ile Cys 
    50                  55                  60                  


Pro Arg Ala Glu Ala Gly Arg Pro Tyr Glu Tyr Tyr Lys Leu Tyr Leu 
65                  70                  75                  80  


Val Arg Pro Glu Gln Ala Ala Ala Cys Ser Thr Val Leu Asp Pro Asn 
                85                  90                  95      


Val Leu Val Thr Cys Asn Lys Pro His Gln Glu Ile Arg Phe Thr Ile 
            100                 105                 110         


Lys Phe Gln Glu Phe Ser Pro Asn Tyr Met Gly Leu Glu Phe Lys Lys 
        115                 120                 125             


Tyr His Asp Tyr Tyr Ile Thr Ser Thr Ser Asn Gly Ser Leu Glu Gly 
    130                 135                 140                 


Leu Glu Asn Arg Glu Gly Gly Val Cys Arg Thr Arg Thr Met Lys Ile 
145                 150                 155                 160 


Val Met Lys Val Gly Gln Asp Pro Asn Ala Val Thr Pro Glu Gln Leu 
                165                 170                 175     


Thr Thr Ser Arg Pro Ser Lys Glu Ser Asp Asn Thr Val Lys Thr Ala 
            180                 185                 190         


Thr Gln Ala Pro Gly Arg Gly Ser Gln Gly Asp Ser Asp Gly Lys His 
        195                 200                 205             


Glu Thr Val Asn Gln Glu Glu Lys Ser Gly Pro Gly Ala Gly Gly Gly 
    210                 215                 220                 


Gly Ser Gly Asp Ser Asp Ser Phe Phe Asn Ser Lys 
225                 230                 235     


<210>  4
<211>  235
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  4

Met Ala Arg Pro Gly Gln Arg Trp Leu Gly Lys Trp Leu Val Ala Met 
1               5                   10                  15      


Val Val Trp Ala Leu Cys Arg Leu Ala Thr Pro Leu Ala Lys Asn Leu 
            20                  25                  30          


Glu Pro Val Ser Trp Ser Ser Leu Asn Pro Lys Phe Leu Ser Gly Lys 
        35                  40                  45              


Gly Leu Val Ile Tyr Pro Lys Ile Gly Asp Lys Leu Asp Ile Ile Cys 
    50                  55                  60                  


Pro Arg Ala Glu Arg Pro Tyr Glu Tyr Tyr Lys Leu Tyr Leu Val Arg 
65                  70                  75                  80  


Pro Glu Gln Ala Ala Ala Cys Ser Thr Val Leu Asp Pro Asn Val Leu 
                85                  90                  95      


Val Thr Cys Asn Arg Pro Glu Gln Glu Ile Arg Phe Thr Ile Lys Phe 
            100                 105                 110         


Gln Glu Phe Ser Pro Asn Tyr Met Gly Leu Glu Phe Lys Lys His His 
        115                 120                 125             


Asp Tyr Tyr Ile Thr Ser Thr Ser Asn Gly Ser Leu Glu Gly Leu Glu 
    130                 135                 140                 


Asn Arg Glu Gly Gly Val Cys Arg Thr Arg Thr Met Lys Ile Ile Met 
145                 150                 155                 160 


Lys Val Gly Gln Asp Pro Asn Ala Val Thr Pro Glu Gln Leu Thr Thr 
                165                 170                 175     


Ser Arg Pro Ser Lys Glu Ala Asp Asn Thr Val Lys Met Ala Thr Gln 
            180                 185                 190         


Ala Pro Gly Ser Arg Gly Ser Leu Gly Asp Ser Asp Gly Lys His Glu 
        195                 200                 205             


Thr Val Asn Gln Glu Glu Lys Ser Gly Pro Gly Ala Ser Gly Gly Ser 
    210                 215                 220                 


Ser Gly Asp Pro Asp Gly Phe Phe Asn Ser Lys 
225                 230                 235 


<210>  5
<211>  221
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  5

Met Ser Asp Gly Asp Tyr Asp Tyr Leu Ile Lys Phe Leu Ala Leu Gly 
1               5                   10                  15      


Asp Ser Gly Val Gly Lys Thr Ser Val Leu Tyr Gln Tyr Thr Asp Gly 
            20                  25                  30          


Lys Phe Asn Ser Lys Phe Ile Thr Thr Val Gly Ile Asp Phe Arg Glu 
        35                  40                  45              


Lys Arg Val Val Tyr Arg Ala Ser Gly Pro Asp Gly Ala Thr Gly Arg 
    50                  55                  60                  


Gly Gln Arg Ile His Leu Gln Leu Trp Asp Thr Ala Gly Gln Glu Arg 
65                  70                  75                  80  


Phe Arg Ser Leu Thr Thr Ala Phe Phe Arg Asp Ala Met Gly Phe Leu 
                85                  90                  95      


Leu Leu Phe Asp Leu Thr Asn Glu Gln Ser Phe Leu Asn Val Arg Asn 
            100                 105                 110         


Trp Ile Ser Gln Leu Gln Met His Ala Tyr Cys Glu Asn Pro Asp Ile 
        115                 120                 125             


Val Leu Cys Gly Asn Lys Ser Asp Leu Glu Asp Gln Arg Val Val Lys 
    130                 135                 140                 


Glu Glu Glu Ala Ile Ala Leu Ala Glu Lys Tyr Gly Ile Pro Tyr Phe 
145                 150                 155                 160 


Glu Thr Ser Ala Ala Asn Gly Thr Asn Ile Ser Gln Ala Ile Glu Met 
                165                 170                 175     


Leu Leu Asp Leu Ile Met Lys Arg Met Glu Arg Cys Val Asp Lys Ser 
            180                 185                 190         


Trp Ile Pro Glu Gly Val Val Arg Ser Asn Gly His Ala Ser Thr Asp 
        195                 200                 205             


Gln Leu Ser Glu Glu Lys Glu Lys Gly Ala Cys Gly Cys 
    210                 215                 220     


<210>  6
<211>  221
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  6

Met Ser Asp Gly Asp Tyr Asp Tyr Leu Ile Lys Phe Leu Ala Leu Gly 
1               5                   10                  15      


Asp Ser Gly Val Gly Lys Thr Ser Val Leu Tyr Gln Tyr Thr Asp Gly 
            20                  25                  30          


Lys Phe Asn Ser Lys Phe Ile Thr Thr Val Gly Ile Asp Phe Arg Glu 
        35                  40                  45              


Lys Arg Val Val Tyr Arg Ala Asn Gly Pro Asp Gly Ala Val Gly Arg 
    50                  55                  60                  


Gly Gln Arg Ile His Leu Gln Leu Trp Asp Thr Ala Gly Gln Glu Arg 
65                  70                  75                  80  


Phe Arg Ser Leu Thr Thr Ala Phe Phe Arg Asp Ala Met Gly Phe Leu 
                85                  90                  95      


Leu Leu Phe Asp Leu Thr Asn Glu Gln Ser Phe Leu Asn Val Arg Asn 
            100                 105                 110         


Trp Ile Ser Gln Leu Gln Met His Ala Tyr Cys Glu Asn Pro Asp Ile 
        115                 120                 125             


Val Leu Cys Gly Asn Lys Ser Asp Leu Glu Asp Gln Arg Ala Val Lys 
    130                 135                 140                 


Glu Glu Glu Ala Arg Glu Leu Ala Glu Lys Tyr Gly Ile Pro Tyr Phe 
145                 150                 155                 160 


Glu Thr Ser Ala Ala Asn Gly Thr Asn Ile Ser His Ala Ile Glu Met 
                165                 170                 175     


Leu Leu Asp Leu Ile Met Lys Arg Met Glu Arg Cys Val Asp Lys Ser 
            180                 185                 190         


Trp Ile Pro Glu Gly Val Val Arg Ser Asn Gly His Thr Ser Ala Asp 
        195                 200                 205             


Gln Leu Ser Glu Glu Lys Glu Lys Gly Leu Cys Gly Cys 
    210                 215                 220     


<210>  7
<211>  218
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  7

Met Thr Asp Gly Asp Tyr Asp Tyr Leu Ile Lys Leu Leu Ala Leu Gly 
1               5                   10                  15      


Asp Ser Gly Val Gly Lys Thr Thr Phe Leu Tyr Arg Tyr Thr Asp Asn 
            20                  25                  30          


Lys Phe Asn Pro Lys Phe Ile Thr Thr Val Gly Ile Asp Phe Arg Glu 
        35                  40                  45              


Lys Arg Val Val Tyr Asn Ala Gln Gly Pro Asn Gly Ser Ser Gly Lys 
    50                  55                  60                  


Ala Phe Lys Val His Leu Gln Leu Trp Asp Thr Ala Gly Gln Glu Arg 
65                  70                  75                  80  


Phe Arg Ser Leu Thr Thr Ala Phe Phe Arg Asp Ala Met Gly Phe Leu 
                85                  90                  95      


Leu Met Phe Asp Leu Thr Ser Gln Gln Ser Phe Leu Asn Val Arg Asn 
            100                 105                 110         


Trp Met Ser Gln Leu Gln Ala Asn Ala Tyr Cys Glu Asn Pro Asp Ile 
        115                 120                 125             


Val Leu Ile Gly Asn Lys Ala Asp Leu Pro Asp Gln Arg Glu Val Asn 
    130                 135                 140                 


Glu Arg Gln Ala Arg Glu Leu Ala Asp Lys Tyr Gly Ile Pro Tyr Phe 
145                 150                 155                 160 


Glu Thr Ser Ala Ala Thr Gly Gln Asn Val Glu Lys Ala Val Glu Thr 
                165                 170                 175     


Leu Leu Asp Leu Ile Met Lys Arg Met Glu Gln Cys Val Glu Lys Thr 
            180                 185                 190         


Gln Ile Pro Asp Thr Val Asn Gly Gly Asn Ser Gly Asn Leu Asp Gly 
        195                 200                 205             


Glu Lys Pro Pro Glu Lys Lys Cys Ile Cys 
    210                 215             


<210>  8
<211>  218
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  8

Met Thr Asp Gly Asp Tyr Asp Tyr Leu Ile Lys Leu Leu Ala Leu Gly 
1               5                   10                  15      


Asp Ser Gly Val Gly Lys Thr Thr Phe Leu Tyr Arg Tyr Thr Asp Asn 
            20                  25                  30          


Lys Phe Asn Pro Lys Phe Ile Thr Thr Val Gly Ile Asp Phe Arg Glu 
        35                  40                  45              


Lys Arg Val Val Tyr Asp Thr Gln Gly Ala Asp Gly Ala Ser Gly Lys 
    50                  55                  60                  


Ala Phe Lys Val His Leu Gln Leu Trp Asp Thr Ala Gly Gln Glu Arg 
65                  70                  75                  80  


Phe Arg Ser Leu Thr Thr Ala Phe Phe Arg Asp Ala Met Gly Phe Leu 
                85                  90                  95      


Leu Met Phe Asp Leu Thr Ser Gln Gln Ser Phe Leu Asn Val Arg Asn 
            100                 105                 110         


Trp Met Ser Gln Leu Gln Ala Asn Ala Tyr Cys Glu Asn Pro Asp Ile 
        115                 120                 125             


Val Leu Ile Gly Asn Lys Ala Asp Leu Pro Asp Gln Arg Glu Val Asn 
    130                 135                 140                 


Glu Arg Gln Ala Arg Glu Leu Ala Glu Lys Tyr Gly Ile Pro Tyr Phe 
145                 150                 155                 160 


Glu Thr Ser Ala Ala Thr Gly Gln Asn Val Glu Lys Ser Val Glu Thr 
                165                 170                 175     


Leu Leu Asp Leu Ile Met Lys Arg Met Glu Lys Cys Val Glu Lys Thr 
            180                 185                 190         


Gln Val Pro Asp Thr Val Asn Gly Gly Asn Ser Gly Lys Leu Asp Gly 
        195                 200                 205             


Glu Lys Pro Ala Glu Lys Lys Cys Ala Cys 
    210                 215             


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  9
atcctgaagt gcattctgcc                                                   20


<210>  10
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  10
tagggtactg agcgagagg                                                    19


<210>  11
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  11
tggccttcac tgtcatagc                                                    19


<210>  12
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  12
ttccaggccc atgtagttg                                                    19


