                         SEQUENCE LISTING

<110>  BRANDEIS UNIVERSITY
 
<120>  DNA DISPLAY OF FOLDED RNA LIBRARIES ENABLING RNA-SELEX WITHOUT 
       REVERSE TRANSCRIPTION

<130>  43022.0381

<150>  62/460,365
<151>  2017-02-17

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Capture Strand 5' Sequence

<400>  1
gctcgttctc cttccctctc c                                                 21


<210>  2
<211>  25
<212>  RNA
<213>  Artificial

<220>
<223>  Clone 1 thrombin binding sequence

<400>  2
uguuacucac aaaauagcga agacu                                             25


<210>  3
<211>  25
<212>  RNA
<213>  Artificial

<220>
<223>  Clone 2 thrombin binding sequence

<400>  3
ccggcgucac aagauagaca aaacu                                             25


<210>  4
<211>  25
<212>  RNA
<213>  Artificial

<220>
<223>  Clone 3 thrombin binding sequence

<400>  4
cgggacucac aagauagaca auacu                                             25


<210>  5
<211>  25
<212>  RNA
<213>  Artificial

<220>
<223>  Clone 4 thrombin binding sequence

<400>  5
ugcggauaac aagauagcaa agacu                                             25


<210>  6
<211>  25
<212>  RNA
<213>  Artificial

<220>
<223>  Clone 5 thrombin binding sequence

<400>  6
cuaccguaag agaacgagca gacuc                                             25


<210>  7
<211>  25
<212>  RNA
<213>  Artificial

<220>
<223>  Clone 6 thrombin binding sequence

<400>  7
cgggacacua aggaacauaa aaguu                                             25


<210>  8
<211>  25
<212>  RNA
<213>  Artificial

<220>
<223>  Clone 7 thrombin binding sequence

<400>  8
ccgaagcucg gagaagcaca gaagc                                             25


<210>  9
<211>  25
<212>  RNA
<213>  Artificial

<220>
<223>  Clone 8 thrombin binding sequence

<400>  9
gggauugcac aagauagcgu agacu                                             25


<210>  10
<211>  25
<212>  RNA
<213>  Artificial

<220>
<223>  Clone 9 thrombin binding sequence

<400>  10
ggguguucac aagauagagu agacu                                             25


<210>  11
<211>  25
<212>  RNA
<213>  Artificial

<220>
<223>  Clone 10 thrombin binding sequence

<400>  11
gcuuugacac aagauacaau auagu                                             25


<210>  12
<211>  87
<212>  DNA
<213>  Artificial

<220>
<223>  Random Library Sequence


<220>
<221>  misc_feature
<222>  (19)..(43)
<223>  Where N at positions 19-43 can be A, T, C or G

<400>  12
ccgggctttg tgtcacttnn nnnnnnnnnn nnnnnnnnnn nnngctcgtt ctccttccct       60

ctcctatagt gagtcgtatt acagttg                                           87


<210>  13
<211>  87
<212>  DNA
<213>  Artificial

<220>
<223>  NheI Library

<400>  13
ccgggctttg tgtcacttta cgttcttatg ttctcactcg ctagctcgtt ctccttccct       60

ctcctatagt gagtcgtatt acagttg                                           87


<210>  14
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  Biotinylated T7 Forward Primer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Where C at position 1 is Biotinylated

<400>  14
caactgtaat acgactcact ataggaga                                          28


<210>  15
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  IsodC Reverse Primer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Where N is IsodC

<400>  15
nccgggcttt gtgtcactt                                                    19


<210>  16
<211>  82
<212>  DNA
<213>  Artificial

<220>
<223>  Capture Strand Rigidifying Oligonucleotide

<400>  16
gcttgttcgt tgtgtggggc actcatgtct aaatcgtttg tctatgcttg cgtcatttct       60

gggtatactg gtctgtggtg aa                                                82


<210>  17
<211>  15
<212>  DNA
<213>  Artificial

<220>
<223>  NheI Selection Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Where the T at position 1 is Biotinylated

<400>  17
tgttctcact cgcta                                                        15


<210>  18
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  T7 Forward Primer

<400>  18
caactgtaat acgactcact ataggaga                                          28


<210>  19
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  Reverse Primer

<400>  19
ccgggctttg tgtcactt                                                     18


<210>  20
<211>  64
<212>  RNA
<213>  Artificial

<220>
<223>  Full length clone 1 aptamer

<400>  20
ggagagagaa ggagaacgag cuguuacuca caaaauagcg aagacuaagu gacacaaagc       60

ccgg                                                                    64


<210>  21
<211>  64
<212>  RNA
<213>  Artificial

<220>
<223>  Full length clone 2 aptamer

<400>  21
ggagagguaa guagaacgag cccggcguca caagauagac aaaacuaagu gacacaaagc       60

ccgg                                                                    64


<210>  22
<211>  64
<212>  RNA
<213>  Artificial

<220>
<223>  Full length clone 3 aptamer

<400>  22
ggagagggaa ggagaacgaa ccgggacuca caagauagac aauacuaagu gacacaaagc       60

ccgg                                                                    64


<210>  23
<211>  63
<212>  RNA
<213>  Artificial

<220>
<223>  Full length clone 4 aptamer

<400>  23
ggagagggaa ggagaacggc ugcggauaac aagauagcaa agacuaagug acacaaagcc       60

cgg                                                                     63


<210>  24
<211>  63
<212>  RNA
<213>  Artificial

<220>
<223>  Full length clone 5 aptamer

<400>  24
ggagagguag gagaacgagc cuaccguaag agaacgagca gacucaagug acacaaagcc       60

cgg                                                                     63


<210>  25
<211>  64
<212>  RNA
<213>  Artificial

<220>
<223>  Full length clone 6 aptamer

<400>  25
ggagagggaa ggagaacgag ccgggacacu aaggaacaua aaaguuaagu gacacaaagc       60

ccgg                                                                    64


<210>  26
<211>  64
<212>  RNA
<213>  Artificial

<220>
<223>  Full length clone 7 aptamer

<400>  26
ggagagggaa ugauaacgag cccgaagcuc ggagaagcac agaagcaagu gacacaaagc       60

ccgg                                                                    64


<210>  27
<211>  63
<212>  RNA
<213>  Artificial

<220>
<223>  Full length clone 8 aptamer

<400>  27
ggagagcgaa gagaacgagc gggauugcac aagauagcgu agacuaagug acacaaagcc       60

cgg                                                                     63


<210>  28
<211>  64
<212>  RNA
<213>  Artificial

<220>
<223>  Full length clone 9 aptamer

<400>  28
ggagagggaa agagaacgag cggguguuca caagauagag uagacuaagu gacacaaagc       60

ccgg                                                                    64


<210>  29
<211>  64
<212>  RNA
<213>  Artificial

<220>
<223>  Full length clone 10 aptamer

<400>  29
ggagacggaa ggauaacgag cgcuuugaca caagauacaa uauaguaagu gacaccaagc       60

ccgg                                                                    64


<210>  30
<211>  25
<212>  RNA
<213>  Artificial

<220>
<223>  Toggle 25 aptamer

<400>  30
gggaacaaag cugaaguacu uaccc                                             25


<210>  31
<211>  124
<212>  DNA
<213>  Artificial

<220>
<223>  Portion of Capture Strand located 5' to linker, for 
       non-fluorinated library

<400>  31
gctcgttctc cttccctctc cttttttttt tcaacaccac agaccagtat acccagaaat       60

gacgcaagca tagacaaacg atttagacat gagtgcccca cacaacgaac aagctttttt      120

ttta                                                                   124


<210>  32
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  Portion of Capture Strand located 3' to linker, for 
       non-fluorinated library

<400>  32
caactgtaat acgactcact ataggaga                                          28


<210>  33
<211>  96
<212>  DNA
<213>  Artificial

<220>
<223>  Random Library Sequence for incorporation of modified RNA


<220>
<221>  misc_feature
<222>  (19)..(43)
<223>  n is a, c, g, or t

<400>  33
ccgggctttg tgtcgcttnn nnnnnnnnnn nnnnnnnnnn nnncttcctc tccctctccc       60

ttcctcttcc tcctatagtg agtcgtatta cagttg                                 96


<210>  34
<211>  124
<212>  DNA
<213>  Artificial

<220>
<223>  Portion of Capture Strand located 5' to linker, for fluorinated 
       library

<400>  34
cctctccctt cctcttcctc cttttttttt tcaacaccac agaccagtat acccagaaat       60

gacgcaagca tagacaaacg atttagacat gagtgcccca cacaacgaac aagctttttt      120

ttta                                                                   124


<210>  35
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  Portion of Capture Strand located 3' to linker, for fluorinated 
       library

<400>  35
caactgtaat acgactcact ataggagg                                          28


