                         SEQUENCE LISTING

<110>  TAKARA BIO USA, INC.
       BOSTICK, Magnolia
       MANN, Ishminder
       FARMER, Andrew Alan
       TAYLOR, Sarah Alan
 
<120>  Methods of Preparing Nucleic Acid Libraries and Compositions and 
       Kits for Practicing the Same

<130>  CLON-141WO

<140>  PCT/US2018/018057
<141>  2018-02-13

<150>  62/459,858
<151>  2017-02-16

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  142
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Protein

<400>  1

Pro Asn Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser 
1               5                   10                  15      


Lys Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln 
            20                  25                  30          


Thr Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys 
        35                  40                  45              


Thr Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val 
    50                  55                  60                  


Ala Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn 
65                  70                  75                  80  


Ser Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys 
                85                  90                  95      


Asp Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn 
            100                 105                 110         


Phe Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val 
        115                 120                 125             


Ala Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
    130                 135                 140         


<210>  2
<211>  429
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Protein

<400>  2
ccaaatatcc agaaccctga ccctgccgtg taccagctga gagactctaa atccagtgac       60

aagtctgtct gcctattcac cgattttgat tctcaaacaa atgtgtcaca aagtaaggat      120

tctgatgtgt atatcacaga caaaactgtg ctagacatga ggtctatgga cttcaagagc      180

aacagtgctg tggcctggag caacaaatct gactttgcat gtgcaaacgc cttcaacaac      240

agcattattc cagaagacac cttcttcccc agcccagaaa gttcctgtga tgtcaagctg      300

gtcgagaaaa gctttgaaac agatacgaac ctaaactttc aaaacctgtc agtgattggg      360

ttccgaatcc tcctcctgaa agtggccggg tttaatctgc tcatgacgct gcggctgtgg      420

tccagctga                                                              429


<210>  3
<211>  138
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Protein

<400>  3

Pro Tyr Ile Gln Asn Pro Glu Pro Ala Val Tyr Gln Leu Lys Asp Pro 
1               5                   10                  15      


Arg Ser Gln Asp Ser Thr Leu Cys Leu Phe Thr Asp Phe Asp Ser Gln 
            20                  25                  30          


Ile Asn Val Pro Lys Thr Met Glu Ser Gly Thr Phe Ile Thr Asp Lys 
        35                  40                  45              


Thr Val Leu Asp Met Lys Ala Met Asp Ser Lys Ser Asn Gly Ala Ile 
    50                  55                  60                  


Ala Trp Ser Asn Gln Thr Ser Phe Thr Cys Gln Asp Ile Phe Lys Glu 
65                  70                  75                  80  


Thr Asn Ala Thr Tyr Pro Ser Ser Asp Val Pro Cys Asp Ala Thr Leu 
                85                  90                  95      


Thr Glu Lys Ser Phe Glu Thr Asp Met Asn Leu Asn Phe Gln Asn Leu 
            100                 105                 110         


Ser Val Met Gly Leu Arg Ile Leu Leu Leu Lys Val Ala Gly Phe Asn 
        115                 120                 125             


Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
    130                 135             


<210>  4
<211>  138
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Protein

<400>  4

Pro Asn Ile Gln Asn Pro Glu Pro Ala Val Tyr Gln Leu Lys Asp Pro 
1               5                   10                  15      


Arg Ser Gln Asp Ser Thr Leu Cys Leu Phe Thr Asp Phe Asp Ser Gln 
            20                  25                  30          


Ile Asn Val Pro Lys Thr Met Glu Ser Gly Thr Phe Ile Thr Asp Lys 
        35                  40                  45              


Thr Val Leu Asp Met Lys Ala Met Asp Ser Lys Ser Asn Gly Ala Ile 
    50                  55                  60                  


Ala Trp Ser Asn Gln Thr Ser Phe Thr Cys Gln Asp Ile Phe Lys Glu 
65                  70                  75                  80  


Thr Asn Ala Thr Tyr Pro Ser Ser Asp Val Pro Cys Asp Ala Thr Leu 
                85                  90                  95      


Thr Glu Lys Ser Phe Glu Thr Asp Met Asn Leu Asn Phe Gln Asn Leu 
            100                 105                 110         


Ser Val Met Gly Leu Arg Ile Leu Leu Leu Lys Val Ala Gly Phe Asn 
        115                 120                 125             


Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
    130                 135             


<210>  5
<211>  414
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Protein

<400>  5
ccatacatcc agaacccaga acctgctgtg taccagttaa aagatcctcg gtctcaggac       60

agcaccctct gcctgttcac cgactttgac tcccaaatca atgtgccgaa aaccatggaa      120

tctggaacgt tcatcactga caaaactgtg ctggacatga aagctatgga ttccaagagc      180

aatggggcca ttgcctggag caaccagaca agcttcacct gccaagatat cttcaaagag      240

accaacgcca cctaccccag ttcagacgtt ccctgtgatg ccacgttgac cgagaaaagc      300

tttgaaacag atatgaacct aaactttcaa aacctgtcag ttatgggact ccgaatcctc      360

ctgctgaaag tagcgggatt taacctgctc atgacgctga ggctgtggtc cagt            414


<210>  6
<211>  414
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Protein

<400>  6
ccaaacatcc agaacccaga acctgctgtg taccagttaa aagatcctcg gtctcaggac       60

agcaccctct gcctgttcac cgactttgac tcccaaatca atgtgccgaa aaccatggaa      120

tctggaacgt tcatcactga caaaactgtg ctggacatga aagctatgga ttccaagagc      180

aatggggcca ttgcctggag caaccagaca agcttcacct gccaagatat cttcaaagag      240

accaacgcca cctaccccag ttcagacgtt ccctgtgatg ccacgttgac cgagaaaagc      300

tttgaaacag atatgaacct aaactttcaa aacctgtcag ttatgggact ccgaatcctc      360

ctgctgaaag tagcgggatt taacctgctc atgacgctga ggctgtggtc cagt            414


<210>  7
<211>  116
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Protein


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (41)..(41)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (56)..(56)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (58)..(58)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (66)..(66)
<223>  n is a, c, g, or t

<400>  7
dnkvvavsas htkatvcatg dhvswwvngk vhsgvstdka ndsrycssrr vsatwnrnhr       60

cvygsndwtd rakvtvsaaw gradcgtsvs ygvsatygka tyavvsavma mvkrkd          116


<210>  8
<211>  531
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Protein

<400>  8
gaggacctga acaaggtgtt cccacccgag gtcgctgtgt ttgagccatc agaagcagag       60

atctcccaca cccaaaaggc cacactggtg tgcctggcca caggcttctt ccccgaccac      120

gtggagctga gctggtgggt gaatgggaag gaggtgcaca gtggggtcag cacagacccg      180

cagcccctca aggagcagcc cgccctcaat gactccagat actgcctgag cagccgcctg      240

agggtctcgg ccaccttctg gcagaacccc cgcaaccact tccgctgtca agtccagttc      300

tacgggctct cggagaatga cgagtggacc caggataggg ccaaacccgt cacccagatc      360

gtcagcgccg aggcctgggg tagagcagac tgtggcttta cctcggtgtc ctaccagcaa      420

ggggtcctgt ctgccaccat cctctatgag atcctgctag ggaaggccac cctgtatgct      480

gtgctggtca gcgcccttgt gttgatggcc atggtcaaga gaaaggattt c               531


<210>  9
<211>  119
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Protein


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (42)..(42)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (57)..(57)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (59)..(59)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (67)..(67)
<223>  n is a, c, g, or t

<400>  9
dknvvavsas htkatvcatg ydhvswwvng kvhsgvstdk andsrycssr rvsatwnrnh       60

rcvygsndwt drakvtvsaa wgradcgtss ygvsatygka tyavvsavma mvkrkdsrg       119


<210>  10
<211>  537
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Protein

<400>  10
gacctgaaaa acgtgttccc acccgaggtc gctgtgtttg agccatcaga agcagagatc       60

tcccacaccc aaaaggccac actggtatgc ctggccacag gcttctaccc cgaccacgtg      120

gagctgagct ggtgggtgaa tgggaaggag gtgcacagtg gggtcagcac agacccgcag      180

cccctcaagg agcagcccgc cctcaatgac tccagatact gcctgagcag ccgcctgagg      240

gtctcggcca ccttctggca gaacccccgc aaccacttcc gctgtcaagt ccagttctac      300

gggctctcgg agaatgacga gtggacccag gatagggcca aacccgtcac ccagatcgtc      360

agcgccgagg cctggggtag agcagactgt ggcttcacct ccgagtctta ccagcaaggg      420

gtcctgtctg ccaccatcct ctatgagatc ttgctaggga aggccacctt gtatgccgtg      480

ctggtcagtg ccctcgtgct gatggccatg gtcaagagaa aggattccag aggctag         537


<210>  11
<211>  120
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Protein


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (45)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (60)..(60)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (62)..(62)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (78)..(78)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (119)..(119)
<223>  n is a, c, g, or t

<400>  11
drnvtkvssk aankkatvca rgdhvswwvn gkvhsgvstd ayksnysycs srrvsatwhn       60

rnhrcvhgsd kwgskvtnsa awgradcgts asygvsatyg katyavvstv vmamvkrkns      120


<210>  12
<211>  522
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Protein

<400>  12
gaggatctga gaaatgtgac tccacccaag gtctccttgt ttgagccatc aaaagcagag       60

attgcaaaca aacaaaaggc taccctcgtg tgcttggcca ggggcttctt ccctgaccac      120

gtggagctga gctggtgggt gaatggcaag gaggtccaca gtggggtcag cacggaccct      180

caggcctaca aggagagcaa ttatagctac tgcctgagca gccgcctgag ggtctctgct      240

accttctggc acaatcctcg caaccacttc cgctgccaag tgcagttcca tgggctttca      300

gaggaggaca agtggccaga gggctcaccc aaacctgtca cacagaacat cagtgcagag      360

gcctggggcc gagcagactg tgggattacc tcagcatcct atcaacaagg ggtcttgtct      420

gccaccatcc tctatgagat cctgctaggg aaagccaccc tgtatgctgt gcttgtcagt      480

acactggtgg tgatggctat ggtcaaaaga aagaattcat ga                         522


<210>  13
<211>  120
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Protein


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (45)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (60)..(60)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (62)..(62)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (78)..(78)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (119)..(119)
<223>  n is a, c, g, or t

<400>  13
drnvtkvssk aankkatvca rgdhvswwvn gkvhsgvstd ayksnysycs srrvsatwhn       60

rnhrcvhgsd kwgskvtnsa awgradcgts asyhgvsaty gkatyavvsg vmamvkkkns      120


<210>  14
<211>  522
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Protein

<400>  14
gaggatctga gaaatgtgac tccacccaag gtctccttgt ttgagccatc aaaagcagag       60

attgcaaaca aacaaaaggc taccctcgtg tgcttggcca ggggcttctt ccctgaccac      120

gtggagctga gctggtgggt gaatggcaag gaggtccaca gtggggtcag cacggaccct      180

caggcctaca aggagagcaa ttatagctac tgcctgagca gccgcctgag ggtctctgct      240

accttctggc acaatcctcg aaaccacttc cgctgccaag tgcagttcca tgggctttca      300

gaggaggaca agtggccaga gggctcaccc aaacctgtca cacagaacat cagtgcagag      360

gcctggggcc gagcagactg tggaatcact tcagcatcct atcatcaggg ggttctgtct      420

gcaaccatcc tctatgagat cctactgggg aaggccaccc tatatgctgt gctggtcagt      480

ggcctggtgc tgatggccat ggtcaagaaa aaaaattcct ga                         522


<210>  15
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Protein

<400>  15
agatgtgtat aagagacag                                                    19


<210>  16
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Protein

<400>  16
ctgtctctta tacacatct                                                    19


<210>  17
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Protein

<400>  17
aatgatacgg cgaccaccga                                                   20


<210>  18
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Protein

<400>  18
caagcagaag acggcatacg agat                                              24


<210>  19
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Protein

<400>  19
acactctttc cctacacgac gctcttccga tct                                    33


<210>  20
<211>  34
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Protein

<400>  20
gtgactggag ttcagacgtg tgctcttccg atct                                   34


<210>  21
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Protein

<400>  21
ccatctcatc cctgcgtgtc tccgactcag                                        30


<210>  22
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Protein

<400>  22
cctctctatg ggcagtcggt gat                                               23


