                               SEQUENCE LISTING

<110> BIOPROCESSIA TECHNOLOGIES LLC
 
<120> AFFINITY CHROMATOGRAPHY LIGANDS WITH MILD ELUTION PH

<130> 44604-0002WO1

<140> PCT/US2017/025335
<141> 2017-03-31

<150> 62/459,394
<151> 2017-02-15

<160> 22    

<170> PatentIn version 3.5

<210> 1
<211> 58
<212> PRT
<213> Staphylococcus aureus

<400> 1
Ala Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
    50                  55              


<210> 2
<211> 58
<212> PRT
<213> Staphylococcus aureus

<400> 2
Ala Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Gly Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys Asp Asp Pro Ser Val Ser Lys Glu Ile Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
    50                  55              


<210> 3
<211> 58
<212> PRT
<213> Staphylococcus aureus

<400> 3
Ala Asp Asn Asn Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu Asn Met Pro Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ser Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys 
    50                  55              


<210> 4
<211> 60
<212> PRT
<213> Staphylococcus aureus

<400> 4
Ala Ala Asn Ala Ala Gln His Asp Glu Ala Gln Gln Asn Ala Phe Tyr 
1               5                   10                  15      


Gln Val Leu Asn Met Pro Asn Leu Asn Ala Asp Gln Arg Asn Gly Phe 
            20                  25                  30          


Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Val Leu Gly 
        35                  40                  45              


Glu Ala Gln Lys Leu Asn Asp Ser Gln Ala Pro Lys 
    50                  55                  60  


<210> 5
<211> 61
<212> PRT
<213> Staphylococcus aureus

<400> 5
Ala Asp Ala Gln Gln Asn Asn Phe Asn Lys Asp Gln Gln Ser Ala Phe 
1               5                   10                  15      


Tyr Glu Ile Leu Asn Met Pro Asn Leu Asn Glu Ala Gln Arg Asn Gly 
            20                  25                  30          


Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Thr Asn Val Leu 
        35                  40                  45              


Gly Glu Ala Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys 
    50                  55                  60      


<210> 6
<211> 58
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 6
Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
    50                  55              


<210> 7
<211> 58
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 7
Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys His Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
    50                  55              


<210> 8
<211> 18
<212> PRT
<213> Bacillus stearothermophilus

<400> 8
Glu Ala Gln Lys Gln Lys Glu Gln Arg Gln Ala Ala Glu Glu Leu Ala 
1               5                   10                  15      


Asn Ala 
        


<210> 9
<211> 301
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 9
Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Val Asp Asn Lys Phe Asn 
    50                  55                  60                  


Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu 
65                  70                  75                  80  


Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro 
                85                  90                  95      


Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala 
            100                 105                 110         


Gln Ala Pro Lys Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala 
        115                 120                 125             


Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn 
    130                 135                 140                 


Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu 
145                 150                 155                 160 


Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Val Asp 
                165                 170                 175     


Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His 
            180                 185                 190         


Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu 
        195                 200                 205             


Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys 
    210                 215                 220                 


Leu Asn Asp Ala Gln Ala Pro Lys Val Asp Asn Lys Phe Asn Lys Glu 
225                 230                 235                 240 


Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu 
                245                 250                 255     


Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln 
            260                 265                 270         


Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala 
        275                 280                 285             


Pro Lys His His His His His His His Cys Cys Lys Cys 
    290                 295                 300     


<210> 10
<211> 391
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 10
Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Glu Ala Gln Lys Gln Lys 
    50                  55                  60                  


Glu Gln Arg Gln Ala Ala Glu Glu Leu Ala Asn Ala Val Asp Asn Lys 
65                  70                  75                  80  


Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro 
                85                  90                  95      


Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp 
            100                 105                 110         


Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn 
        115                 120                 125             


Asp Ala Gln Ala Pro Lys Glu Ala Gln Lys Gln Lys Glu Gln Arg Gln 
    130                 135                 140                 


Ala Ala Glu Glu Leu Ala Asn Ala Val Asp Asn Lys Phe Asn Lys Glu 
145                 150                 155                 160 


Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu 
                165                 170                 175     


Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln 
            180                 185                 190         


Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala 
        195                 200                 205             


Pro Lys Glu Ala Gln Lys Gln Lys Glu Gln Arg Gln Ala Ala Glu Glu 
    210                 215                 220                 


Leu Ala Asn Ala Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala 
225                 230                 235                 240 


Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn 
                245                 250                 255     


Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu 
            260                 265                 270         


Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Glu Ala 
        275                 280                 285             


Gln Lys Gln Lys Glu Gln Arg Gln Ala Ala Glu Glu Leu Ala Asn Ala 
    290                 295                 300                 


Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile 
305                 310                 315                 320 


Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln 
                325                 330                 335     


Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala 
            340                 345                 350         


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Glu Ala Gln Lys Gln Lys 
        355                 360                 365             


Glu Gln Arg Gln Ala Ala Glu Glu Leu Ala Asn Ala His His His His 
    370                 375                 380                 


His His His Cys Cys Lys Cys 
385                 390     


<210> 11
<211> 301
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 11
Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys His Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Val Asp Asn Lys Phe Asn 
    50                  55                  60                  


Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu 
65                  70                  75                  80  


Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys His Asp Pro 
                85                  90                  95      


Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala 
            100                 105                 110         


Gln Ala Pro Lys Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala 
        115                 120                 125             


Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn 
    130                 135                 140                 


Ala Phe Ile Gln Ser Leu Lys His Asp Pro Ser Gln Ser Ala Asn Leu 
145                 150                 155                 160 


Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Val Asp 
                165                 170                 175     


Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His 
            180                 185                 190         


Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu 
        195                 200                 205             


Lys His Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys 
    210                 215                 220                 


Leu Asn Asp Ala Gln Ala Pro Lys Val Asp Asn Lys Phe Asn Lys Glu 
225                 230                 235                 240 


Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu 
                245                 250                 255     


Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys His Asp Pro Ser Gln 
            260                 265                 270         


Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala 
        275                 280                 285             


Pro Lys His His His His His His His Cys Cys Lys Cys 
    290                 295                 300     


<210> 12
<211> 243
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 12
Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Val Asp Asn Lys Phe Asn 
    50                  55                  60                  


Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu 
65                  70                  75                  80  


Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro 
                85                  90                  95      


Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala 
            100                 105                 110         


Gln Ala Pro Lys Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala 
        115                 120                 125             


Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn 
    130                 135                 140                 


Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu 
145                 150                 155                 160 


Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Val Asp 
                165                 170                 175     


Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His 
            180                 185                 190         


Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu 
        195                 200                 205             


Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys 
    210                 215                 220                 


Leu Asn Asp Ala Gln Ala Pro Lys His His His His His His His Cys 
225                 230                 235                 240 


Cys Lys Cys 
            


<210> 13
<211> 297
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 13
Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Glu Ala Gln Lys Gln Lys 
    50                  55                  60                  


Glu Gln Arg Gln Ala Ala Glu Glu Leu Ala Asn Ala Val Asp Asn Lys 
65                  70                  75                  80  


Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro 
                85                  90                  95      


Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp 
            100                 105                 110         


Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn 
        115                 120                 125             


Asp Ala Gln Ala Pro Lys Glu Ala Gln Lys Gln Lys Glu Gln Arg Gln 
    130                 135                 140                 


Ala Ala Glu Glu Leu Ala Asn Ala Val Asp Asn Lys Phe Asn Lys Glu 
145                 150                 155                 160 


Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu 
                165                 170                 175     


Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln 
            180                 185                 190         


Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala 
        195                 200                 205             


Pro Lys Glu Ala Gln Lys Gln Lys Glu Gln Arg Gln Ala Ala Glu Glu 
    210                 215                 220                 


Leu Ala Asn Ala Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala 
225                 230                 235                 240 


Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn 
                245                 250                 255     


Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu 
            260                 265                 270         


Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys His His 
        275                 280                 285             


His His His His His Cys Cys Lys Cys 
    290                 295         


<210> 14
<211> 20
<212> PRT
<213> Homo sapiens

<400> 14
Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Ser Arg Arg Ala Gln Asp Phe 
1               5                   10                  15      


Val Gln Trp Leu 
            20  


<210> 15
<211> 23
<212> PRT
<213> Paramecium tetraurelia

<400> 15
Phe Leu Ser Leu Met Ala Arg Lys Met Lys Glu Gln Asp Ser Glu Glu 
1               5                   10                  15      


Glu Leu Ile Glu Ala Phe Lys 
            20              


<210> 16
<211> 27
<212> PRT
<213> Homo sapiens

<400> 16
Glu Ile Asp Ser Thr Trp Ser Ala Leu Glu Lys Ala Glu Gln Glu His 
1               5                   10                  15      


Ala Glu Ala Leu Arg Ile Glu Leu Lys Arg Gln 
            20                  25          


<210> 17
<211> 75
<212> PRT
<213> Homo sapiens

<400> 17
Glu Gly Glu Tyr Ile Lys Leu Lys Val Ile Gly Gln Asp Ser Ser Glu 
1               5                   10                  15      


Ile His Phe Lys Val Lys Met Thr Thr His Leu Lys Lys Leu Lys Glu 
            20                  25                  30          


Ser Tyr Cys Gln Arg Gln Gly Val Pro Met Asn Ser Leu Arg Phe Leu 
        35                  40                  45              


Phe Glu Gly Gln Arg Ile Ala Asp Asn His Thr Pro Lys Glu Leu Gly 
    50                  55                  60                  


Met Glu Glu Glu Asp Val Ile Glu Val Tyr Gln 
65                  70                  75  


<210> 18
<211> 48
<212> PRT
<213> Homo sapiens

<400> 18
Asn Ser Tyr Pro Gly Cys Pro Ser Ser Tyr Asp Gly Tyr Cys Leu Asn 
1               5                   10                  15      


Gly Gly Val Cys Met His Ile Glu Ser Leu Asp Ser Tyr Thr Cys Asn 
            20                  25                  30          


Cys Val Ile Gly Tyr Ser Gly Asp Arg Cys Gln Thr Arg Asp Leu Arg 
        35                  40                  45              


<210> 19
<211> 40
<212> PRT
<213> Homo sapiens

<400> 19
Ser Glu Tyr Glu Thr Met Leu Thr Glu Ile Met Ser Met Gly Tyr Glu 
1               5                   10                  15      


Arg Glu Arg Val Val Ala Ala Leu Arg Ala Ser Tyr Asn Asn Pro His 
            20                  25                  30          


Arg Ala Val Glu Tyr Leu Leu Thr 
        35                  40  


<210> 20
<211> 70
<212> PRT
<213> Streptococcus dysgalactiae

<400> 20
Thr Tyr Lys Leu Ile Leu Asn Gly Lys Thr Leu Lys Gly Glu Thr Thr 
1               5                   10                  15      


Thr Glu Ala Val Asp Ala Ala Thr Ala Glu Lys Val Phe Lys Gln Tyr 
            20                  25                  30          


Ala Asn Asp Asn Gly Val Asp Gly Glu Trp Thr Tyr Asp Asp Ala Thr 
        35                  40                  45              


Lys Thr Phe Thr Val Thr Glu Lys Pro Glu Val Ile Asp Ala Ser Glu 
    50                  55                  60                  


Leu Thr Pro Ala Val Thr 
65                  70  


<210> 21
<211> 55
<212> PRT
<213> Streptococcus dysgalactiae

<400> 21
Thr Tyr Lys Leu Val Ile Asn Gly Lys Thr Leu Lys Gly Glu Thr Thr 
1               5                   10                  15      


Thr Lys Ala Val Asp Ala Glu Thr Ala Glu Lys Ala Phe Lys Gln Tyr 
            20                  25                  30          


Ala Asn Asp Asn Gly Val Asp Gly Val Trp Thr Tyr Asp Asp Ala Thr 
        35                  40                  45              


Lys Thr Phe Thr Val Thr Glu 
    50                  55  


<210> 22
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 22
Gly Gly Gly Gly Gly Gly 
1               5       


