Organization Applicant
----------------------
      
<110> OrganizationName : De vita Bio life sciences

Application Project
-------------------
<120> Title : An Expression vector
<130> AppFileReference : PCT00123
<140> CurrentAppNumber : 
<141> CurrentFilingDate : ____-__-__

Sequence
--------
<213> OrganismName : Homo sapiens
<400> PreSequenceString : 
ggtaccaata tatttaatat atttagttta tatacatcta cagataaata catatcatat        60

atttgaattc taatctccct ctcaacccta cagtcaccca tttggtatat taaagatgtg       120

ttgtctactg tctagtatcc ctcaagcagt gtcaggaatt agtcatttaa atagtctgca       180

agccaggagt ggtggctcat gtctgtaatt ccagcacttg agaggtagaa gtgggaggac       240

tgcttgagct caagagtttg atattatcct ggacaacata gcaagacctc gtctctactt       300

aaaaaaaaaa aattagccag gcatgtgatg tacacctgta gtcccagcta ctcaggaggc       360

cgaaatggga ggatcagatc tggatcc                                           387
<212> Type : DNA
<211> Length : 387
      SequenceName : Sequence ID 1: Short MARS
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Homo sapiens
<400> PreSequenceString : 
aagcttctga caaattattc ttcctcttta ggttctcctt tatggaatct tctgtactga        60

tggccatgtc ctttaactac tatgtagata tctgctacta cctgtattat gcctctacct       120

ttattagcag agttatctgt actgttggca tgacaatcat ttgttaatat gacttgcctt       180

tcctttttct gctattcttg atcaaatggc tcctctttct tgctcctctc atttctcctg       240

ccttcacttg gacgtgcttc acgtagtctg tgcttatgac tggattaaaa attgatatgg       300

acttatccta atgttgttcg tcataatatg ggttttatgg tccattatta tttcctatgc       360

attgatctgg agaaggcttc aatcctttta ctctttgtgg aaaatatctg taaaccttct       420

ggttcactct gctatagcaa tttcagttta ggctagtaag catgaggatg cctccttctc       480

tgatttttcc cacagtctgt tggtcacaga ataacctgag tgattactga tgaaagagtg       540

agaatgttat tgatagtcac aatgacaaaa aacaaacaac tacagtcaaa atgtttctct       600

ttttattagt ggttatattt cctgacctat atctggcagg actctttaga gaggtagctg       660

aagctgctgt tatgaccact agagggaaga agatacctgt ggagctaatg gtccaagatg       720

gtggagcccc aagcaaggaa gttgttaagg agcccttttg attgaaggtg ggtgccccca       780

ccttacaggg acaggacatc tggatactcc tcccagtttc tccagtttcc ctttttccta       840

atatatctcc tgataaaatg tctatactca cttccccatt tctaataata aagcaaaggc       900

tagttagtaa gacatcacct tgcattttga aaatgccata gactttcaaa attatttcat       960

acatcggtct ttctttattt caagagtcca gaaatggcaa cattaccttt gattcaatgt      1020

aatggaaaga gctctttcaa gagacagaga aaagaataat ttaatttctt tccccacacc      1080

tccttccctg tctcttaccc tatcttcctt ccttctaccc tccccatttc tctctctcat      1140

ttctcagaag tatattttga aaggattcat agcagacagc taaggctggt tttttctaag      1200

tgaagaagtg atattgagaa ggtagggttg catgagccct ttcagttttt tagtttatat      1260

acatctgtat tgttagaatg ttttataata taaataaaat tatttctcag ttatatacta      1320

gctatgtaac ctgtggatat ttccttaagt attacaagct atacttaact cacttggaaa      1380

actcaaataa atacctgctt catagttatt aataaggatt aagtgagata atgccctata      1440

agattcctat taataacaga taaatacata cacacacaca cacattgaaa ggattcttac      1500

tttgtgctag gaactataat aagttcattg atgcattata tcattaagtt ctaatttcaa      1560

cactagaagg caggtattat ctaaatttca tactggatac ctccaaactc ataaagataa      1620

ttaaattgcc ttttgtcata tatttattca aaagggtaac tcaaactatg gcttgtctaa      1680

ttttatatat caccctactg aacatgaccc tattgtgata ttttataaaa ttatctcaag      1740

ttattatgag gatgttgaaa gacagagagg atgggtgcta tgccccaaat cagcctcaca      1800

attaagctaa gcagctaaga gtcttgcagg gtagtgtagg gaccacaggg ttaagggggc      1860

agtagaatta tactcccact ttagtttcat ttcaaacaat ccatacacac acagccctga      1920

gcacttacaa attatactac gctctatact ttttgtttaa atgtataaat aagtggatga      1980

aagaatagat agatagacag atagatgata gatagaataa atgcttgcct tcatagctgt      2040

ctccctacct tgttcaaaat gttcctgtcc agaccaaagt accttgcctt cacttaagta      2100

atcaattcct aggttatatt ctgatgtcaa aggaagtcaa aagatgtgaa aaacaatttc      2160

tgacccacaa ctcatgcttt gtagatgact agatcaaaaa atttcagcca tatcttaaca      2220

gtgagtgaac aggaaatctc ctcttttccc tacatctgag atcccagctt ctaagacctt      2280

caattctcac tcttgatgca acagaccttg gaagtataca ggagagctga acttggtcaa      2340

caaaggagaa aagtttgttg gcctccaaag gcacagctca aacttttcaa gccttctcta      2400

atcttaaagg taaacaaggg tctcatttct ttgagaactt cagggaaaat agacaaggac      2460

ttgcctggtg cttttggtag gggagcttgc actttccccc tttctggagg aaatatttat      2520

ccccaggtag ttcccttttt gcaccagtgg ttctttgaag agacttccac ctgggaacag      2580

ttaaacagca actacagggc cttgaactgc acactttcag tccggtcctc acagttgaaa      2640

agacctaagc ttgtgcctga tttaagcctt tttggtcata aaacattgaa ttctaatctc      2700

cctctcaacc ctacagtcac ccatttggta tattaaagat gtgttgtcta ctgtctagta      2760

tccctcaagc agtgtcagga attagtcatt taaatagtct gcaagccagg agtggtggct      2820

catgtctgta attccagcac ttgagaggta gaagtgggag gactgcttga gctcaagagt      2880

ttgatattat cctggacaac atagcaagac ctcgtctcta cttaaaaaaa aaaaattagc      2940

caggcatgtg atgtacacct gtagtcccag ctactcagga ggccgaaatg ggaggatcc       2999


<212> Type : DNA
<211> Length : 2999
      SequenceName : Sequence ID 2: Human beta-globin MARS
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Homo sapiens
<400> PreSequenceString : 
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag        60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga       120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat       180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga       240

cgaaacacca gttctcc                                                      257
<212> Type : DNA
<211> Length : 257
      SequenceName : Sequence ID 3: U6 promoter
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
tttactggag cagctgcag                                                     19
<212> Type : DNA
<211> Length : 19
      SequenceName : Sequence ID 4: BakshRNA sense sequence
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
ctgcagctgc tccagtaaa                                                     19
<212> Type : DNA
<211> Length : 19
      SequenceName : Sequence ID 5: BakshRNA anti sense sequence
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
tcagaaccat catgggctg                                                     19
<212> Type : DNA
<211> Length : 19
      SequenceName : Sequence ID 6: BaxshRNA sense sequence 
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
cagcccatga tggttctga                                                     19
<212> Type : DNA
<211> Length : 19
      SequenceName : Sequence ID 7: BaxshRNA anti sense sequence 
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Homo sapiens
<400> PreSequenceString : 
gtaagtatca aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga        60

cagagaagac tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc       120

tttctctcca cag                                                          133
<212> Type : DNA
<211> Length : 133
      SequenceName : Sequence ID 8: chimeric intron 
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Homo sapiens
<400> PreSequenceString : 
gccgccacca tgg                                                           13
<212> Type : DNA
<211> Length : 13
      SequenceName : Sequence ID 9: kozak sequence 
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
gtgtccactc ccagttcaat tacagctctt aaggctagag tact                         44
<212> Type : DNA
<211> Length : 44
      SequenceName : Sequence ID 10: linker sequence 
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
atgggctggt cctgcatcat cctgttcctg gtggccaccg ccaccggcgt gcactcc           57


<212> Type : DNA
<211> Length : 57
      SequenceName : Sequence ID 11: signal peptide
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Encephalomyocarditis virus
<400> PreSequenceString : 
cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg        60

tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg       120

gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg       180

aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca       240

aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct       300

ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca       360

cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa       420

ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg       480

cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg       540

gacgtggttt tcctttgaaa aacacgatga taatatg                                577
<212> Type : DNA
<211> Length : 577
      SequenceName : Sequence ID 12: EMCV IRES 
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
atggttcgac cgctgaactg catcgtggcc gtgtcccaga atatgggcat cggcaagaac        60

ggagattttc cctggccaat gctcaggaac gaattcaagt acttccaaag aatgaccacc       120

acctcctcag tggaaggtaa acagaacttg gtgattatgg gccggaaaac ttggttctcc       180

attcctgaga agaatcgacc tttaaaggac agaattaata tagttctcag tagagagctc       240

aaggaaccac cacaaggagc tcattttctt gccaaaagtc tggacgatgc cttaaaactt       300

attgaacaac cagagttagc agataaagtg gacatggttt ggatagttgg aggcagttcc       360

gtttacaagg aagccatgaa tcagccaggc catctcagac tctttgtgac aaggatcatg       420

caggaatttg aaactgacac gttcttccca gaaattgatt tggagaaata taaacttctc       480

ccagagtacc caagggtcct tcctgaagtc caagaggaaa aaggcatcaa gtataaattt       540

gaagtctatg agaagaaagg c                                                 561
<212> Type : DNA
<211> Length : 561
      SequenceName : Sequence ID 13: DHFR sequence
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
cggcggaagc gg                                                            12
<212> Type : DNA
<211> Length : 12
      SequenceName : Sequence ID 14: Furin recognition site
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
ggctccggc                                                                 9
<212> Type : DNA
<211> Length : 9
      SequenceName : Sequence ID 15: GSG linker
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
gagggccggg gctccctgct gacctgcggc gacgtggagg agaaccccgg cccc              54


<212> Type : DNA
<211> Length : 54
      SequenceName : Sequence ID 16: T2A site
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
gacatccaga tgacccagtc cccctcctcc ctgtccgcct ccgtgggcga ccgggtgacc        60

atcacctgcc gggcctccca ggacgtgaac accgccgtgg cctggtacca gcagaagccc       120

ggcaaggccc ccaagctgct gatctactcc gcctccttcc tgtactccgg cgtgccctcc       180

cggttctccg gctcccggtc cggcaccgac ttcaccctga ccatctcctc cctgcagccc       240

gaggacttcg ccacctacta ctgccagcag cactacacca ccccccccac cttcggccag       300

ggcaccaagg tggagatcaa gcggaccgtg gccgccccct ccgtgttcat cttccccccc       360

tccgacgagc agctgaagtc cggcaccgcc tccgtggtgt gcctgctgaa caacttctac       420

ccccgggagg ccaaggtgca gtggaaggtg gacaacgccc tgcagtccgg caactcccag       480

gagtccgtga ccgagcagga ctccaaggac tccacctact ccctgtcctc caccctgacc       540

ctgtccaagg ccgactacga gaagcacaag gtgtacgcct gcgaggtgac ccaccagggc       600

ctgtcctccc ccgtgaccaa gtccttcaac cggggcgagt gc                          642
<212> Type : DNA
<211> Length : 642
      SequenceName : Sequence ID 17: Trastuzumab light chain nucleotide sequence
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
diqmtqspss lsasvgdrvt itcrasqdvn tavawyqqkp gkapklliys asflysgvps        60

rfsgsrsgtd ftltisslqp edfatyycqq hyttpptfgq gtkveikrtv aapsvfifpp       120

sdeqlksgta svvcllnnfy preakvqwkv dnalqsgnsq esvteqdskd styslsstlt       180

lskadyekhk vyacevthqg lsspvtksfn rgec                                   214
<212> Type : DNA
<211> Length : 214
      SequenceName : Sequence ID 18: Trastuzumab light chain protein sequence
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
gaggtgcagc tggtggagtc cggcggcggc ctggtgcagc ccggcggctc cctgcggctg        60

tcctgcgccg cctccggctt caacatcaag gacacctaca tccactgggt gcggcaggcc       120

cccggcaagg gcctggagtg ggtggcccgg atctacccca ccaacggcta cacccggtac       180

gccgactccg tgaagggccg gttcaccatc tccgccgaca cctccaagaa caccgcctac       240

ctgcagatga actccctgcg ggccgaggac accgccgtgt actactgctc ccggtggggc       300

ggcgacggct tctacgccat ggactactgg ggccagggca ccctggtgac cgtgtcctcc       360

gcctccacca agggcccctc cgtgttcccc ctggccccct cctccaagtc cacctccggc       420

ggcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgtcc       480

tggaactccg gcgccctgac ctccggcgtg cacaccttcc ccgccgtgct gcagtcctcc       540

ggcctgtact ccctgtcctc cgtggtgacc gtgccctcct cctccctggg cacccagacc       600

tacatctgca acgtgaacca caagccctcc aacaccaagg tggacaagaa ggtggagccc       660

aagtcctgcg acaagaccca cacctgcccc ccctgccccg cccccgagct gctgggcggc       720

ccctccgtgt tcctgttccc ccccaagccc aaggacaccc tgatgatctc ccggaccccc       780

gaggtgacct gcgtggtggt ggacgtgtcc cacgaggacc ccgaggtgaa gttcaactgg       840

tacgtggacg gcgtggaggt gcacaacgcc aagaccaagc cccgggagga gcagtacaac       900

tccacctacc gggtggtgtc cgtgctgacc gtgctgcacc aggactggct gaacggcaag       960

gagtacaagt gcaaggtgtc caacaaggcc ctgcccgccc ccatcgagaa gaccatctcc      1020

aaggccaagg gccagccccg ggagccccag gtgtacaccc tgcccccctc ccgggaggag      1080

atgaccaaga accaggtgtc cctgacctgc ctggtgaagg gcttctaccc ctccgacatc      1140

gccgtggagt gggagtccaa cggccagccc gagaacaact acaagaccac cccccccgtg      1200

ctggactccg acggctcctt cttcctgtac tccaagctga ccgtggacaa gtcccggtgg      1260

cagcagggca acgtgttctc ctgctccgtg atgcacgagg ccctgcacaa ccactacacc      1320

cagaagtccc tgtccctgtc ccccggcaag                                       1350
<212> Type : DNA
<211> Length : 1350
      SequenceName : Sequence ID 19: Trastuzumab heavy chain nucleotide sequence
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
evqlvesggg lvqpggslrl scaasgfnik dtyihwvrqa pgkglewvar iyptngytry        60

adsvkgrfti sadtskntay lqmnslraed tavyycsrwg gdgfyamdyw gqgtlvtvss       120

astkgpsvfp lapsskstsg gtaalgclvk dyfpepvtvs wnsgaltsgv htfpavlqss       180

glyslssvvt vpssslgtqt yicnvnhkps ntkvdkkvep kscdkthtcp pcpapellgg       240

psvflfppkp kdtlmisrtp evtcvvvdvs hedpevkfnw yvdgvevhna ktkpreeqyn       300

styrvvsvlt vlhqdwlngk eykckvsnka lpapiektis kakgqprepq vytlppsree       360

mtknqvsltc lvkgfypsdi avewesngqp ennykttppv ldsdgsffly skltvdksrw       420

qqgnvfscsv mhealhnhyt qkslslspgk                                        450
<212> Type : DNA
<211> Length : 450
      SequenceName : Sequence ID 20: Trastuzumab heavy chain protein sequence
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
ccgccgagat ctgagggcct atttcccatg attccttcat atttgcatat acgatacaag        60

gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat       120

acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa       180

atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc       240

ttgtggaaag gacgaaacac cagttctcct ttactggagc agctgcagtt caagagactg       300

cagctgctcc agtaaatttt ttggaaaagc tagtgtccac tcccagttca attacagctc       360

ttaaggctag agtactgagg gcctatttcc catgattcct tcatatttgc atatacgata       420

caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca       480

aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt       540

taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata       600

tatcttgtgg aaaggacgaa acaccagttc tcctcagaac catcatgggc tgttcaagag       660

acagcccatg atggttctga ttttttggaa agatctccgc cg                          702
<212> Type : DNA
<211> Length : 702
      SequenceName : Sequence ID 21: shRNA Cassette
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
ccgccggcta gcgtaagtat caaggttaca agacaggttt aaggagacca atagaaactg        60

ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac       120

atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc       180

tagagtactg ccgccaccat gggctggtcc tgcatcatcc tgttcctggt ggccaccgcc       240

accggcgtgc actccgacat ccagatgacc cagtccccct cctccctgtc cgcctccgtg       300

ggcgaccggg tgaccatcac ctgccgggcc tcccaggacg tgaacaccgc cgtggcctgg       360

taccagcaga agcccggcaa ggcccccaag ctgctgatct actccgcctc cttcctgtac       420

tccggcgtgc cctcccggtt ctccggctcc cggtccggca ccgacttcac cctgaccatc       480

tcctccctgc agcccgagga cttcgccacc tactactgcc agcagcacta caccaccccc       540

cccaccttcg gccagggcac caaggtggag atcaagcgga ccgtggccgc cccctccgtg       600

ttcatcttcc ccccctccga cgagcagctg aagtccggca ccgcctccgt ggtgtgcctg       660

ctgaacaact tctacccccg ggaggccaag gtgcagtgga aggtggacaa cgccctgcag       720

tccggcaact cccaggagtc cgtgaccgag caggactcca aggactccac ctactccctg       780

tcctccaccc tgaccctgtc caaggccgac tacgagaagc acaaggtgta cgcctgcgag       840

gtgacccacc agggcctgtc ctcccccgtg accaagtcct tcaaccgggg cgagtgctag       900

gcggccgccc gccg                                                         914
<212> Type : DNA
<211> Length : 914
      SequenceName : Sequence ID 22: Chimeric intron fused with signal peptide followed by Trastuzumab light chain 
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
ccgccgctcg aggtgtccac tcccagttca attacagctc ttaaggctag agtactcccc        60

tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg       120

tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa       180

cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg       240

caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca       300

acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc       360

ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt       420

gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg       480

ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca       540

tgctttacat gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac cacggggacg       600

tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatgggctg gtcctgcatc       660

atcctgttcc tggtggccac cgccaccggc gtgcactccg aggtgcagct ggtggagtcc       720

ggcggcggcc tggtgcagcc cggcggctcc ctgcggctgt cctgcgccgc ctccggcttc       780

aacatcaagg acacctacat ccactgggtg cggcaggccc ccggcaaggg cctggagtgg       840

gtggcccgga tctaccccac caacggctac acccggtacg ccgactccgt gaagggccgg       900

ttcaccatct ccgccgacac ctccaagaac accgcctacc tgcagatgaa ctccctgcgg       960

gccgaggaca ccgccgtgta ctactgctcc cggtggggcg gcgacggctt ctacgccatg      1020

gactactggg gccagggcac cctggtgacc gtgtcctccg cctccaccaa gggcccctcc      1080

gtgttccccc tggccccctc ctccaagtcc acctccggcg gcaccgccgc cctgggctgc      1140

ctggtgaagg actacttccc cgagcccgtg accgtgtcct ggaactccgg cgccctgacc      1200

tccggcgtgc acaccttccc cgccgtgctg cagtcctccg gcctgtactc cctgtcctcc      1260

gtggtgaccg tgccctcctc ctccctgggc acccagacct acatctgcaa cgtgaaccac      1320

aagccctcca acaccaaggt ggacaagaag gtggagccca agtcctgcga caagacccac      1380

acctgccccc cctgccccgc ccccgagctg ctgggcggcc cctccgtgtt cctgttcccc      1440

cccaagccca aggacaccct gatgatctcc cggacccccg aggtgacctg cgtggtggtg      1500

gacgtgtccc acgaggaccc cgaggtgaag ttcaactggt acgtggacgg cgtggaggtg      1560

cacaacgcca agaccaagcc ccgggaggag cagtacaact ccacctaccg ggtggtgtcc      1620

gtgctgaccg tgctgcacca ggactggctg aacggcaagg agtacaagtg caaggtgtcc      1680

aacaaggccc tgcccgcccc catcgagaag accatctcca aggccaaggg ccagccccgg      1740

gagccccagg tgtacaccct gcccccctcc cgggaggaga tgaccaagaa ccaggtgtcc      1800

ctgacctgcc tggtgaaggg cttctacccc tccgacatcg ccgtggagtg ggagtccaac      1860

ggccagcccg agaacaacta caagaccacc ccccccgtgc tggactccga cggctccttc      1920

ttcctgtact ccaagctgac cgtggacaag tcccggtggc agcagggcaa cgtgttctcc      1980

tgctccgtga tgcacgaggc cctgcacaac cactacaccc agaagtccct gtccctgtcc      2040

cccggcaagt agctcgagcc gccg                                             2064
<212> Type : DNA
<211> Length : 2064
      SequenceName : Sequence ID 23: EMCV IRES fused with Trastuzumab Heavy Chain
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
ccgccgtcta gagtgtccac tcccagttca attacagctc ttaaggctag agtactcccc        60

tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg       120

tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa       180

cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg       240

caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca       300

acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc       360

ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt       420

gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg       480

ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca       540

tgctttacat gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac cacggggacg       600

tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggttcg accgctgaac       660

tgcatcgtgg ccgtgtccca gaatatgggc atcggcaaga acggagattt tccctggcca       720

atgctcagga acgaattcaa gtacttccaa agaatgacca ccacctcctc agtggaaggt       780

aaacagaact tggtgattat gggccggaaa acttggttct ccattcctga gaagaatcga       840

cctttaaagg acagaattaa tatagttctc agtagagagc tcaaggaacc accacaagga       900

gctcattttc ttgccaaaag tctggacgat gccttaaaac ttattgaaca accagagtta       960

gcagataaag tggacatggt ttggatagtt ggaggcagtt ccgtttacaa ggaagccatg      1020

aatcagccag gccatctcag actctttgtg acaaggatca tgcaggaatt tgaaactgac      1080

acgttcttcc cagaaattga tttggagaaa tataaacttc tcccagagta cccaagggtc      1140

cttcctgaag tccaagagga aaaaggcatc aagtataaat ttgaagtcta tgagaagaaa      1200

ggctaatcta gaccgccg                                                    1218
<212> Type : DNA
<211> Length : 1218
      SequenceName : Sequence ID 24: EMCV IRES fused with DHFR
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
ccgccgctcg aggtgtccac tcccagttca attacagctc ttaaggctag agtactcccc        60

tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg       120

tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa       180

cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg       240

caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca       300

acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc       360

ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt       420

gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg       480

ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca       540

tgctttacat gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac cacggggacg       600

tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggttcg accgctgaac       660

tgcatcgtgg ccgtgtccca gaatatgggc atcggcaaga acggagattt tccctggcca       720

atgctcagga acgaattcaa gtacttccaa agaatgacca ccacctcctc agtggaaggt       780

aaacagaact tggtgattat gggccggaaa acttggttct ccattcctga gaagaatcga       840

cctttaaagg acagaattaa tatagttctc agtagagagc tcaaggaacc accacaagga       900

gctcattttc ttgccaaaag tctggacgat gccttaaaac ttattgaaca accagagtta       960

gcagataaag tggacatggt ttggatagtt ggaggcagtt ccgtttacaa ggaagccatg      1020

aatcagccag gccatctcag actctttgtg acaaggatca tgcaggaatt tgaaactgac      1080

acgttcttcc cagaaattga tttggagaaa tataaacttc tcccagagta cccaagggtc      1140

cttcctgaag tccaagagga aaaaggcatc aagtataaat ttgaagtcta tgagaagaaa      1200

ggccggcgga agcggggctc cggcgagggc cggggctccc tgctgacctg cggcgacgtg      1260

gaggagaacc ccggccccat gggctggtcc tgcatcatcc tgttcctggt ggccaccgcc      1320

accggcgtgc actccgaggt gcagctggtg gagtccggcg gcggcctggt gcagcccggc      1380

ggctccctgc ggctgtcctg cgccgcctcc ggcttcaaca tcaaggacac ctacatccac      1440

tgggtgcggc aggcccccgg caagggcctg gagtgggtgg cccggatcta ccccaccaac      1500

ggctacaccc ggtacgccga ctccgtgaag ggccggttca ccatctccgc cgacacctcc      1560

aagaacaccg cctacctgca gatgaactcc ctgcgggccg aggacaccgc cgtgtactac      1620

tgctcccggt ggggcggcga cggcttctac gccatggact actggggcca gggcaccctg      1680

gtgaccgtgt cctccgcctc caccaagggc ccctccgtgt tccccctggc cccctcctcc      1740

aagtccacct ccggcggcac cgccgccctg ggctgcctgg tgaaggacta cttccccgag      1800

cccgtgaccg tgtcctggaa ctccggcgcc ctgacctccg gcgtgcacac cttccccgcc      1860

gtgctgcagt cctccggcct gtactccctg tcctccgtgg tgaccgtgcc ctcctcctcc      1920

ctgggcaccc agacctacat ctgcaacgtg aaccacaagc cctccaacac caaggtggac      1980

aagaaggtgg agcccaagtc ctgcgacaag acccacacct gccccccctg ccccgccccc      2040

gagctgctgg gcggcccctc cgtgttcctg ttccccccca agcccaagga caccctgatg      2100

atctcccgga cccccgaggt gacctgcgtg gtggtggacg tgtcccacga ggaccccgag      2160

gtgaagttca actggtacgt ggacggcgtg gaggtgcaca acgccaagac caagccccgg      2220

gaggagcagt acaactccac ctaccgggtg gtgtccgtgc tgaccgtgct gcaccaggac      2280

tggctgaacg gcaaggagta caagtgcaag gtgtccaaca aggccctgcc cgcccccatc      2340

gagaagacca tctccaaggc caagggccag ccccgggagc cccaggtgta caccctgccc      2400

ccctcccggg aggagatgac caagaaccag gtgtccctga cctgcctggt gaagggcttc      2460

tacccctccg acatcgccgt ggagtgggag tccaacggcc agcccgagaa caactacaag      2520

accacccccc ccgtgctgga ctccgacggc tccttcttcc tgtactccaa gctgaccgtg      2580

gacaagtccc ggtggcagca gggcaacgtg ttctcctgct ccgtgatgca cgaggccctg      2640

cacaaccact acacccagaa gtccctgtcc ctgtcccccg gcaagtagtc tagaccgccg      2700


<212> Type : DNA
<211> Length : 2700
      SequenceName : Sequence ID 25: EMCV IRES fused with DHFR, Furin T2A and Trastuzumab heavy chain
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
ccgccgcaat tgttagtaag acatcacctt gcattttgaa aatgccatag actttcaaaa        60

ttatttcata catcggtctt tctttatttc aagagtccag aaatggcaac attacctttg       120

attcaatgta atggaaagag ctctttcaag agacagagaa aagaataatt taatttcttt       180

ccccacacct ccttccctgt ctcttaccct atcttccttc cttctaccct ccccatttct       240

ctctctcatt tctcagaagt atattttgaa aggattcata gcagacagct aaggctggtt       300

ttttctaagt gaagaagtga tattgagaag gtagggttgc atgagccctt tcagtttttt       360

agtttatata catctgtatt gttagaatgt tttataatat aaataaaatt atttctcagt       420

tatatactag ctatgtaacc tgtggatatt tccttaagta ttacaagcta tacttaactc       480

acttggaaaa ctcaaataaa tacctgcttc atagttatta ataaggatta agtgagataa       540

tgcccataag attcctatta ataacagata aatacataca cacacacaca cattgaaagg       600

attcttactt tgtgctagga actataataa gttcattgat gcattatatc attaagttct       660

aatttcaaca ctagaaggca ggtattatct aaatttcata ctggatacct ccaaactcat       720

aaagataatt aaattgcctt ttgtcatata tttattcaaa agggtaactc aaactatggc       780

tcaattgccg ccg                                                          793
<212> Type : DNA
<211> Length : 793
      SequenceName : Sequence ID 26: human beta globin MARS (short MARS)
      SequenceDescription : 

Sequence
--------
<213> OrganismName : Artificial Sequence
<400> PreSequenceString : 
ccgccgctcg aggtgtccac tcccagttca attacagctc ttaaggctag agtactcccc        60

tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg       120

tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa       180

cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg       240

caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca       300

acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc       360

ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt       420

gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg       480

ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca       540

tgctttacat gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac cacggggacg       600

tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggttcg accgctgaac       660

tgcatcgtgg ccgtgtccca gaatatgggc atcggcaaga acggagattt tccctggcca       720

atgctcagga acgaattcaa gtacttccaa agaatgacca ccacctcctc agtggaaggt       780

aaacagaact tggtgattat gggccggaaa acttggttct ccattcctga gaagaatcga       840

cctttaaagg acagaattaa tatagttctc agtagagagc tcaaggaacc accacaagga       900

gctcattttc ttgccaaaag tctggacgat gccttaaaac ttattgaaca accagagtta       960

gcagataaag tggacatggt ttggatagtt ggaggcagtt ccgtttacaa ggaagccatg      1020

aatcagccag gccatctcag actctttgtg acaaggatca tgcaggaatt tgaaactgac      1080

acgttcttcc cagaaattga tttggagaaa tataaacttc tcccagagta cccaagggtc      1140

cttcctgaag tccaagagga aaaaggcatc aagtataaat ttgaagtcta tgagaagaaa      1200

ggccggcgga agcggggctc cggcgagggc cggggctccc tgctgacctg cggcgacgtg      1260

gaggagaacc ccggccccat gggctggtcc tgcatcatcc tgttcctggt ggccaccgcc      1320

accggcgtgc actccgaggt gcagctggtg gagtccggcg gcggcctggt gcagcccggc      1380

ggctccctgc ggctgtcctg cgccgcctcc ggcttcaaca tcaaggacac ctacatccac      1440

tgggtgcggc aggcccccgg caagggcctg gagtgggtgg cccggatcta ccccaccaac      1500

ggctacaccc ggtacgccga ctccgtgaag ggccggttca ccatctccgc cgacacctcc      1560

aagaacaccg cctacctgca gatgaactcc ctgcgggccg aggacaccgc cgtgtactac      1620

tgctcccggt ggggcggcga cggcttctac gccatggact actggggcca gggcaccctg      1680

gtgaccgtgt cctccgcctc caccaagggc ccctccgtgt tccccctggc cccctcctcc      1740

aagtccacct ccggcggcac cgccgccctg ggctgcctgg tgaaggacta cttccccgag      1800

cccgtgaccg tgtcctggaa ctccggcgcc ctgacctccg gcgtgcacac cttccccgcc      1860

gtgctgcagt cctccggcct gtactccctg tcctccgtgg tgaccgtgcc ctcctcctcc      1920

ctgggcaccc agacctacat ctgcaacgtg aaccacaagc cctccaacac caaggtggac      1980

aagaaggtgg agcccaagtc ctgcgacaag acccacacct gccccccctg ccccgccccc      2040

gagctgctgg gcggcccctc cgtgttcctg ttccccccca agcccaagga caccctgatg      2100

atctcccgga cccccgaggt gacctgcgtg gtggtggacg tgtcccacga ggaccccgag      2160

gtgaagttca actggtacgt ggacggcgtg gaggtgcaca acgccaagac caagccccgg      2220

gaggagcagt acaactccac ctaccgggtg gtgtccgtgc tgaccgtgct gcaccaggac      2280

tggctgaacg gcaaggagta caagtgcaag gtgtccaaca aggccctgcc cgcccccatc      2340

gagaagacca tctccaaggc caagggccag ccccgggagc cccaggtgta caccctgccc      2400

ccctcccggg aggagatgac caagaaccag gtgtccctga cctgcctggt gaagggcttc      2460

tacccctccg acatcgccgt ggagtgggag tccaacggcc agcccgagaa caactacaag      2520

accacccccc ccgtgctgga ctccgacggc tccttcttcc tgtactccaa gctgaccgtg      2580

gacaagtccc ggtggcagca gggcaacgtg ttctcctgct ccgtgatgca cgaggccctg      2640

cacaaccact acacccagaa gtccctgtcc ctgtcccccg gcaagtagtc tagaccgccg      2700


<212> Type : DNA
<211> Length : 2700
      SequenceName : Sequence ID 27: DHFR fused with Trastuzumab heavy chain
      SequenceDescription : 

