                         SEQUENCE LISTING

<110>  Novozymes A/S
       Puratos NV
 
<120>  LIPOLYTIC ENZYME FOR USE IN BAKING

<130>  14303-WO-PCT

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  309
<212>  PRT
<213>  Valsaria rubricosa

<400>  1

Met Lys Ser Ala Ser Ile Leu Leu Arg Val Ala Ala Leu Leu Leu Pro 
1               5                   10                  15      


Ala Val Ser Ala Leu Pro Leu Glu Arg Arg Ala Ile Ser Ala Asp Leu 
            20                  25                  30          


Leu Ala Thr Phe Ser Leu Phe Glu Gln Phe Ala Ala Ala Ala Tyr Cys 
        35                  40                  45              


Pro Asp Asn Asn Asp Ser Pro Asp Thr Lys Leu Thr Cys Ser Val Gly 
    50                  55                  60                  


Asn Cys Pro Leu Val Glu Ala Asp Thr Thr Ser Thr Val Thr Glu Phe 
65                  70                  75                  80  


Glu Asn Ser Leu Glu Thr Asp Val Thr Gly Tyr Val Ala Thr Asp Ser 
                85                  90                  95      


Thr Arg Glu Leu Ile Val Val Ala Phe Arg Gly Ser Ser Ser Ile Arg 
            100                 105                 110         


Asn Trp Ile Ala Asp Ile Asp Phe Pro Phe Thr Asp Thr Asp Leu Cys 
        115                 120                 125             


Asp Gly Cys Gln Ala Ala Ser Gly Phe Trp Thr Ser Trp Thr Glu Ala 
    130                 135                 140                 


Arg Thr Gly Val Leu Ala Ala Val Ala Ser Ala Ala Ala Ala Asn Pro 
145                 150                 155                 160 


Ser Tyr Thr Val Ala Val Thr Gly His Ser Leu Gly Gly Ala Val Ala 
                165                 170                 175     


Ala Leu Ala Ala Gly Ala Leu Arg Asn Ala Gly Tyr Thr Val Ala Leu 
            180                 185                 190         


Tyr Ser Phe Gly Ala Pro Arg Val Gly Asp Glu Thr Leu Ser Glu Tyr 
        195                 200                 205             


Ile Thr Ala Gln Ala Gly Gly Asn Tyr Arg Ile Thr His Leu Asn Asp 
    210                 215                 220                 


Pro Val Pro Lys Leu Pro Pro Leu Leu Leu Gly Tyr Arg His Ile Ser 
225                 230                 235                 240 


Pro Glu Tyr Tyr Ile Ser Ser Gly Asn Asn Val Thr Val Thr Ala Asp 
                245                 250                 255     


Asp Val Glu Glu Tyr Thr Gly Thr Ile Asn Leu Ser Gly Asn Thr Gly 
            260                 265                 270         


Asp Leu Thr Phe Asp Thr Asp Ala His Ser Trp Tyr Phe Asn Glu Ile 
        275                 280                 285             


Gly Ala Cys Asp Asp Gly Glu Ala Leu Glu Trp Lys Lys Arg Gly Val 
    290                 295                 300                 


Glu Val Gln Trp Val 
305                 


<210>  2
<211>  1059
<212>  DNA
<213>  Valsaria rubricosa

<400>  2
atgaagtccg cttcgatctt actcagggta gctgccctcc tcctccctgc tgtatctgca       60

ctgccacttg aaagaagagg tatggacgaa ctatcctagc gatcagtgtg tctattttgc      120

ctaacctagc aaagctatat ccgcggatct cctggcaacc ttcagcctct tcgagcagtt      180

cgcagccgca gcatattgtc cggataacaa cgacagtccc gacaccaagc ttacttgctc      240

tgtcggaaac tgcccgcttg tcgaagctga cacgaccagc acggtcactg aattcgaaaa      300

gtacatctta cacgaccccg ttcacctaca gacaaagtcc cagctaacgt ccacctctat      360

ctctgtccct ttagctcgct cgaaaccgac gtcactggct acgtcgcgac tgacagcaca      420

cgagagctca tcgttgtggc attccgcggg agttcctcga tccggaactg gatcgccgac      480

atcgactttc ccttcaccga caccgacctc tgcgatggct gccaggcagc ctcgggcttc      540

tggacgtcct ggacggaggc acggacaggg gtgctggcgg cggtggcgag cgctgccgcg      600

gccaacccgt cctataccgt tgccgtgacg ggccacagcc tcggcggggc cgtggccgcg      660

ctggccgctg gcgccctccg gaacgcgggc tacacggtcg cgctatacag cttcggagcg      720

cctcgcgtgg gtgacgagac cctcagcgag tacatcactg cgcaggcggg tggaaactac      780

cgcatcacgc acctcaacga cccagtgccg aagctgcccc cgctgctcct ggggtatcgc      840

cacatcagcc cggaatacta catcagcagc gggaacaacg tgaccgtgac ggcggatgac      900

gtggaggagt acaccggcac gatcaacctg agtgggaaca cgggcgatct gacgttcgac      960

acggatgcgc acagttggta cttcaacgag atcggggcat gcgatgatgg tgaggctttg     1020

gagtggaaga agcggggggt agaagttcag tgggtttaa                            1059


<210>  3
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  3
acacaactgg ggatccacca tgaagtccgc ttcgatctta ctcagg                      46


<210>  4
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  4
agatctcgag aagcttaaac ccactgaact tctacccccc                             40


<210>  5
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Catalytic segment

<400>  5

Gly His Ser Leu Gly 
1               5   


<210>  6
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  6

Met Lys Ser Ala Ser Ile Leu Leu Arg Val Ala Ala Leu Leu Leu Pro 
1               5                   10                  15      


Ala Val Ser Ala Leu Pro Leu Glu Arg Arg Ala Ile Ser Ala Asp Leu 
            20                  25                  30          


Leu Ala Thr Phe Ser Leu Phe Glu Gln Phe Ala Ala Ala Ala Tyr Cys 
        35                  40                  45              


Pro Asn Asn Asn Asn Ser Pro Asp Thr Lys Leu Thr Cys Ser Gln Gly 
    50                  55                  60                  


Asn Cys Pro Leu Val Glu Ala Ala Thr Thr Ser Thr Val Thr Glu Phe 
65                  70                  75                  80  


Glu Asn Ser Leu Ser Thr Asp Val Thr Gly Tyr Val Ala Val Asp Ser 
                85                  90                  95      


Thr Arg Glu Leu Ile Val Val Ala Phe Arg Gly Ser Ser Ser Ile Arg 
            100                 105                 110         


Asn Trp Ile Ala Asp Ile Asp Phe Pro Phe Thr Asp Thr Asp Leu Cys 
        115                 120                 125             


Asp Gly Cys Gln Ala Ala Ser Gly Phe Trp Gln Ser Trp Thr Glu Ala 
    130                 135                 140                 


Arg Thr Gly Val Thr Ala Ala Val Ala Ser Ala Ala Ala Gln Asn Pro 
145                 150                 155                 160 


Ser Tyr Thr Val Val Val Thr Gly His Ser Leu Gly Gly Ala Val Ala 
                165                 170                 175     


Ala Leu Ala Ala Gly Ala Leu Arg Asn Gln Gly Tyr Thr Val Ala Leu 
            180                 185                 190         


Tyr Ser Phe Gly Ala Pro Arg Val Gly Asn Glu Thr Leu Ser Glu Tyr 
        195                 200                 205             


Ile Thr Ala Gln Ala Gly Gly Asn Tyr Arg Ile Thr His Leu Asn Asp 
    210                 215                 220                 


Pro Val Pro Lys Leu Pro Pro Leu Leu Leu Gly Tyr Arg His Ile Ser 
225                 230                 235                 240 


Pro Glu Tyr Tyr Ile Ser Ser Gly Asn Asn Val Thr Val Thr Ala Asn 
                245                 250                 255     


Asp Val Glu Glu Tyr Thr Gly Thr Ile Asn Leu Ser Gly Asn Thr Gly 
            260                 265                 270         


Asp Leu Thr Phe Asp Thr Asp Ala His Ser Trp Tyr Phe Asn Glu Ile 
        275                 280                 285             


Gly Ala Cys Asp Asp Gly Glu Ala Leu Glu Trp Lys Lys Arg Gly Val 
    290                 295                 300                 


Glu Val Gln Trp Val 
305                 


<210>  7
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  7

Met Lys Ser Ala Ser Ile Leu Leu Arg Val Ala Ala Leu Leu Leu Pro 
1               5                   10                  15      


Ala Val Ser Ala Leu Pro Leu Glu Arg Arg Ala Ile Ser Ala Asp Leu 
            20                  25                  30          


Leu Ala Thr Phe Ser Leu Phe Glu Gln Phe Ala Ala Ala Ala Tyr Cys 
        35                  40                  45              


Pro Asn Asn Asn Asn Ser Pro Gly Thr Lys Leu Thr Cys Ser Gln Gly 
    50                  55                  60                  


Asn Cys Pro Leu Val Glu Ala Ala Thr Thr Asn Thr Val Thr Glu Phe 
65                  70                  75                  80  


Glu Asn Ser Leu Ser Thr Asp Val Thr Gly Tyr Val Ala Val Asp Ser 
                85                  90                  95      


Thr Asn Glu Leu Ile Val Val Ser Phe Arg Gly Ser Ser Ser Ile Arg 
            100                 105                 110         


Asn Trp Ile Ala Asp Ile Asp Phe Pro Phe Thr Asp Thr Asp Leu Cys 
        115                 120                 125             


Asp Gly Cys Gln Ala Ala Ser Gly Phe Trp Gln Ser Trp Thr Glu Ala 
    130                 135                 140                 


Arg Thr Thr Val Thr Ala Ala Val Ala Gln Ala Ala Ala Gln Asn Pro 
145                 150                 155                 160 


Ser Tyr Gln Val Val Val Thr Gly His Ser Leu Gly Gly Ala Ile Ala 
                165                 170                 175     


Ala Leu Ala Ala Gly Ala Leu Arg Asn Gln Gly Tyr Thr Val Asp Leu 
            180                 185                 190         


Tyr Ser Phe Gly Ala Pro Arg Val Gly Asn Glu Thr Leu Ser Glu Tyr 
        195                 200                 205             


Ile Thr Asn Gln Ala Gly Gly Asn Tyr Arg Ile Thr His Leu Asn Asp 
    210                 215                 220                 


Pro Val Pro Lys Leu Pro Pro Leu Leu Met Gly Tyr Arg His Ile Ser 
225                 230                 235                 240 


Pro Glu Tyr Tyr Ile Ser Ser Gly Asn Asn Val Thr Val Thr Ala Asn 
                245                 250                 255     


Asp Val Gln Glu Tyr Thr Gly Thr Ile Asn Leu Gln Gly Asn Thr Gly 
            260                 265                 270         


Asp Leu Thr Phe Asp Ile Asp Ala His Ser Trp Tyr Phe Asn Glu Ile 
        275                 280                 285             


Gly Ala Cys Asp Asp Gly Glu Ala Leu Glu Trp Lys Lys Arg Gly Val 
    290                 295                 300                 


Glu Val Gln Trp Val 
305                 


<210>  8
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  8

Met Lys Ser Ala Ser Ile Leu Leu Arg Val Ala Ala Leu Leu Leu Pro 
1               5                   10                  15      


Ala Val Ser Ala Leu Pro Leu Glu Arg Arg Ala Ile Ser Ala Asp Leu 
            20                  25                  30          


Leu Ala Thr Phe Gln Phe Phe Glu Gln Tyr Ala Ala Ala Ala Tyr Cys 
        35                  40                  45              


Pro Asn Asn Asn Asn Ser Pro Gly Thr Lys Leu Thr Cys Ser Gln Gly 
    50                  55                  60                  


Asn Cys Pro Leu Val Gln Ala Ala Thr Thr Asn Thr Val Tyr Glu Phe 
65                  70                  75                  80  


Glu Asn Ser Leu Ser Thr Asp Val Thr Gly Tyr Val Ala Val Asp Ser 
                85                  90                  95      


Thr Asn Lys Leu Ile Val Val Ser Phe Arg Gly Ser Ser Ser Ile Arg 
            100                 105                 110         


Asn Trp Ile Ala Asp Ile Asp Phe Pro Phe Thr Asp Thr Asp Leu Cys 
        115                 120                 125             


Asp Gly Cys Gln Ala Ala Ser Gly Phe Trp Gln Ser Trp Leu Glu Ala 
    130                 135                 140                 


Arg Thr Thr Val Thr Pro Ala Val Ala Gln Ala Arg Ala Gln Asn Pro 
145                 150                 155                 160 


Asp Tyr Gln Val Val Val Thr Gly His Ser Leu Gly Gly Ala Ile Ala 
                165                 170                 175     


Ala Leu Ala Ala Gly Asp Leu Arg Asn Gln Gly Tyr Thr Val Asp Leu 
            180                 185                 190         


Tyr Thr Phe Gly Ala Pro Arg Val Gly Asn Glu Thr Leu Ser Glu Tyr 
        195                 200                 205             


Ile Thr Asn Gln Ala Gly Gly Asn Tyr Arg Ile Thr His Trp Asn Asp 
    210                 215                 220                 


Pro Val Pro Lys Leu Pro Pro Leu Leu Met Gly Tyr Val His Ile Ser 
225                 230                 235                 240 


Pro Glu Tyr Tyr Ile Ser Ser Gly Asn Asn Val Thr Val Thr Ala Asn 
                245                 250                 255     


Asp Val Gln Glu Tyr Thr Gly Thr Ile Asn Leu Gln Gly Asn Thr Gly 
            260                 265                 270         


Asp Leu Thr Phe Asp Ile Asp Ala His Ser Trp Tyr Phe Asn Glu Ile 
        275                 280                 285             


Gly Ala Cys Asp Asp Gly Glu Ala Leu Glu Trp Lys Lys Arg Gly Val 
    290                 295                 300                 


Glu Val Gln Trp Val 
305                 


<210>  9
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  9

Met Lys Ser Ala Ser Ile Leu Leu Arg Val Ala Ala Leu Leu Leu Pro 
1               5                   10                  15      


Ala Val Ser Ala Leu Pro Leu Glu Arg Arg Ala Ile Ser Ala Asp Leu 
            20                  25                  30          


Leu Asp Thr Phe Gln Phe Phe Glu Gln Tyr Ala Ala Ala Ala Tyr Cys 
        35                  40                  45              


Pro Asn Asn Asn Asn Ser Pro Gly Thr Lys Leu Thr Cys Ser Gln Gly 
    50                  55                  60                  


Asn Cys Pro Leu Val Gln Ala Ala Asp Thr Asn Thr Val Tyr Glu Phe 
65                  70                  75                  80  


Glu Asn Ser Leu Ser Thr Asp Val Thr Gly Tyr Val Ala Val Asp His 
                85                  90                  95      


Thr Asn Lys Leu Ile Val Val Ser Phe Arg Gly Ser Ser Ser Ile Arg 
            100                 105                 110         


Asn Trp Ile Ala Asp Ile Asp Phe Pro Phe Thr Asp Thr Asp Leu Cys 
        115                 120                 125             


Asp Gly Cys Gln Ala Ala Ser Gly Phe Trp Gln Ser Trp Leu Glu Ala 
    130                 135                 140                 


Arg Asp Thr Val Thr Pro Ala Val Tyr Gln Ala Arg Ala Gln Lys Pro 
145                 150                 155                 160 


Asp Tyr Gln Val Val Val Thr Gly His Ser Leu Gly Gly Ala Ile Ala 
                165                 170                 175     


Ala Leu Ala Ala Gly Asp Leu Arg Asn Gln Gly Tyr Thr Val Asp Leu 
            180                 185                 190         


Tyr Thr Phe Gly Ala Pro Arg Val Gly Asn Ser Thr Leu Ser Glu Tyr 
        195                 200                 205             


Ile Thr Asn Gln Pro Gly Gly Asn Tyr Arg Val Thr His Trp Asn Asp 
    210                 215                 220                 


Pro Val Pro Lys Leu Pro Pro Leu Leu Met Gly Tyr Val His Ile Ser 
225                 230                 235                 240 


Pro Glu Tyr Tyr Ile Ser Ser Pro Asn Asn Val Thr Val Thr Ala Asn 
                245                 250                 255     


Asp Val Gln Val Tyr Glu Gly Val Ile Asn Leu Gln Gly Asn Glu Gly 
            260                 265                 270         


Asp Leu Thr Thr Asp Ile Asp Ala His Ser Trp Tyr Phe Asn Glu Ile 
        275                 280                 285             


Gly Ala Cys Asp Asp Gly Glu Ala Leu Glu Trp Lys Lys Arg Gly Val 
    290                 295                 300                 


Glu Val Gln Trp Val 
305                 


