                         SEQUENCE LISTING

<110>  Alexander Klimka, Oleg Krut, Martin Krnke
 
<120>  Polypeptide epitopes of S. aureus and respective monoclonal 
       antibodies for the treatment of infections and immune-diagnosis

<130>  K31638WO

<150>  EP17156750.6
<151>  2017-02-17

<160>  32    

<170>  PatentIn version 3.5

<210>  1
<211>  466
<212>  PRT
<213>  Staphylococcus aureus

<400>  1

Met Thr Lys Ser Val Ala Ile Ile Gly Ala Gly Ile Thr Gly Leu Ser 
1               5                   10                  15      


Ser Ala Tyr Phe Leu Lys Gln Gln Asp Pro Asn Ile Asp Val Thr Ile 
            20                  25                  30          


Phe Glu Ala Ser Asn Arg Pro Gly Gly Lys Ile Gln Ser Tyr Arg Lys 
        35                  40                  45              


Asp Gly Tyr Met Ile Glu Leu Gly Pro Glu Ser Tyr Leu Gly Arg Lys 
    50                  55                  60                  


Thr Ile Met Thr Glu Leu Ala Lys Asp Ile Gly Leu Glu Gln Asp Ile 
65                  70                  75                  80  


Val Thr Asn Thr Thr Gly Gln Ser Tyr Ile Phe Ala Lys Asn Lys Leu 
                85                  90                  95      


Tyr Pro Ile Pro Gly Gly Ser Ile Met Gly Ile Pro Thr Asp Ile Lys 
            100                 105                 110         


Pro Phe Val Thr Thr Lys Leu Ile Ser Pro Leu Gly Lys Leu Arg Ala 
        115                 120                 125             


Gly Leu Asp Leu Ile Lys Lys Pro Ile Gln Met Gln Asp Gly Asp Ile 
    130                 135                 140                 


Ser Val Gly Ala Phe Phe Arg Ala Arg Leu Gly Asn Glu Val Leu Glu 
145                 150                 155                 160 


Asn Leu Ile Glu Pro Leu Met Gly Gly Ile Tyr Gly Thr Asp Ile Asp 
                165                 170                 175     


Lys Leu Ser Leu Met Ser Thr Phe Pro Asn Phe Lys Glu Lys Glu Glu 
            180                 185                 190         


Ala Phe Gly Ser Leu Ile Lys Gly Met Lys Asp Glu Lys Asn Lys Arg 
        195                 200                 205             


Leu Lys Gln Arg Gln Leu Tyr Pro Gly Ala Pro Lys Gly Gln Phe Lys 
    210                 215                 220                 


Gln Phe Lys His Gly Leu Ser Ser Phe Ile Glu Ala Leu Glu Gln Asp 
225                 230                 235                 240 


Val Lys Asn Lys Gly Val Thr Ile Arg Tyr Asn Thr Ser Val Asp Asp 
                245                 250                 255     


Ile Ile Thr Ser Gln Lys Gln Tyr Lys Ile Val Tyr Ser Asn Gln Gln 
            260                 265                 270         


Glu Asp Val Phe Asp Gly Val Leu Val Thr Thr Pro His Gln Val Phe 
        275                 280                 285             


Leu Asn Trp Phe Gly Gln Asp Pro Ala Phe Asp Tyr Phe Lys Thr Met 
    290                 295                 300                 


Asp Ser Thr Thr Val Ala Thr Val Val Leu Ala Phe Asp Glu Lys Asp 
305                 310                 315                 320 


Ile Glu Asn Thr Tyr Asp Gly Thr Gly Phe Val Ile Ala Arg Thr Ser 
                325                 330                 335     


Asp Thr Asp Ile Thr Ala Cys Thr Trp Thr Ser Lys Lys Trp Pro Phe 
            340                 345                 350         


Thr Thr Pro Glu Gly Lys Val Leu Ile Arg Ala Tyr Val Gly Lys Pro 
        355                 360                 365             


Gly Asp Thr Val Val Asp Asp His Thr Asp Asn Glu Leu Val Ser Ile 
    370                 375                 380                 


Val Arg Arg Asp Leu Ser Gln Met Met Thr Phe Lys Gly Asp Pro Glu 
385                 390                 395                 400 


Phe Thr Ile Val Asn Arg Leu Pro Lys Ser Met Pro Gln Tyr His Val 
                405                 410                 415     


Gly His Ile Gln Gln Ile Arg Gln Ile Gln Ala His Ile Lys Gln Thr 
            420                 425                 430         


Tyr Pro Arg Leu Arg Val Thr Gly Ala Ser Phe Glu Ala Val Gly Leu 
        435                 440                 445             


Pro Asp Cys Ile Thr Gln Gly Lys Val Ala Ala Glu Glu Val Ile Ala 
    450                 455                 460                 


Glu Leu 
465     


<210>  2
<211>  253
<212>  PRT
<213>  Staphylococcus aureus

<400>  2

Met Arg Thr Pro Ile Ile Ala Gly Asn Trp Lys Met Asn Lys Thr Val 
1               5                   10                  15      


Gln Glu Ala Lys Asp Phe Val Asn Ala Leu Pro Thr Leu Pro Asp Ser 
            20                  25                  30          


Lys Glu Val Glu Ser Val Ile Cys Ala Pro Ala Ile Gln Leu Asp Ala 
        35                  40                  45              


Leu Thr Thr Ala Val Lys Glu Gly Lys Ala Gln Gly Leu Glu Ile Gly 
    50                  55                  60                  


Ala Gln Asn Thr Tyr Phe Glu Asp Asn Gly Ala Phe Thr Gly Glu Thr 
65                  70                  75                  80  


Ser Pro Val Ala Leu Ala Asp Leu Gly Val Lys Tyr Val Val Ile Gly 
                85                  90                  95      


His Ser Glu Arg Arg Glu Leu Phe His Glu Thr Asp Glu Glu Ile Asn 
            100                 105                 110         


Lys Lys Ala His Ala Ile Phe Lys His Gly Met Thr Pro Ile Ile Cys 
        115                 120                 125             


Val Gly Glu Thr Asp Glu Glu Arg Glu Ser Gly Lys Ala Asn Asp Val 
    130                 135                 140                 


Val Gly Glu Gln Val Lys Lys Ala Val Ala Gly Leu Ser Glu Asp Gln 
145                 150                 155                 160 


Leu Lys Ser Val Val Ile Ala Tyr Glu Pro Ile Trp Ala Ile Gly Thr 
                165                 170                 175     


Gly Lys Ser Ser Thr Ser Glu Asp Ala Asn Glu Met Cys Ala Phe Val 
            180                 185                 190         


Arg Gln Thr Ile Ala Asp Leu Ser Ser Lys Glu Val Ser Glu Ala Thr 
        195                 200                 205             


Arg Ile Gln Tyr Gly Gly Ser Val Lys Pro Asn Asn Ile Lys Glu Tyr 
    210                 215                 220                 


Met Ala Gln Thr Asp Ile Asp Gly Ala Leu Val Gly Gly Ala Ser Leu 
225                 230                 235                 240 


Lys Val Glu Asp Phe Val Gln Leu Leu Glu Gly Ala Lys 
                245                 250             


<210>  3
<211>  306
<212>  PRT
<213>  Staphylococcus aureus

<400>  3

Met Ile Arg Asn Arg Val Met Asn Ser Val Val Asn Lys Tyr Leu Leu 
1               5                   10                  15      


His Asn Arg Ser Ile Met Phe Lys Asn Asp Gln Asp Val Glu Arg Phe 
            20                  25                  30          


Phe Tyr Lys Arg Glu Ile Glu Asn Arg Lys Lys His Lys Gln Pro Ser 
        35                  40                  45              


Thr Leu Asn Val Lys Ala Asn Leu Glu Lys Leu Ser Leu Asp Asp Met 
    50                  55                  60                  


Gln Val Phe Arg Phe Asn Phe Arg His Gln Ile Asp Lys Lys Ile Leu 
65                  70                  75                  80  


Tyr Ile His Gly Gly Phe Asn Ala Leu Gln Pro Ser Pro Phe His Trp 
                85                  90                  95      


Arg Leu Leu Asp Lys Ile Thr Leu Ser Thr Leu Tyr Glu Val Val Leu 
            100                 105                 110         


Pro Ile Tyr Pro Lys Thr Pro Glu Phe His Ile Asp Asp Thr Phe Gln 
        115                 120                 125             


Ala Ile Gln Arg Val Tyr Asp Gln Leu Val Ser Glu Val Gly His Gln 
    130                 135                 140                 


Asn Val Val Val Met Gly Asp Gly Ser Gly Gly Ala Leu Ala Leu Ser 
145                 150                 155                 160 


Phe Val Gln Ser Leu Leu Asp Asn Gln Gln Pro Leu Pro Asn Lys Leu 
                165                 170                 175     


Tyr Leu Ile Ser Pro Ile Leu Asp Ala Thr Leu Ser Asn Lys Asp Ile 
            180                 185                 190         


Ser Asp Ala Leu Ile Glu Gln Asp Ala Val Leu Ser Gln Phe Gly Val 
        195                 200                 205             


Asn Glu Ile Met Lys Lys Trp Ala Asn Gly Leu Pro Leu Thr Asp Lys 
    210                 215                 220                 


Arg Ile Ser Pro Ile Asn Gly Thr Ile Glu Gly Leu Pro Pro Val Tyr 
225                 230                 235                 240 


Met Phe Gly Gly Gly Arg Glu Met Thr His Pro Asp Met Lys Leu Phe 
                245                 250                 255     


Glu Gln Met Met Leu Gln His His Gln Tyr Ile Glu Phe Tyr Asp Tyr 
            260                 265                 270         


Pro Lys Met Val His Asp Phe Pro Ile Tyr Pro Ile Arg Gln Ser His 
        275                 280                 285             


Lys Ala Ile Lys Gln Ile Ala Lys Ser Ile Asp Glu Asp Val Thr Gln 
    290                 295                 300                 


Asn Asn 
305     


<210>  4
<211>  12
<212>  PRT
<213>  Staphylococcus aureus

<400>  4

Lys Asn Asp Gln Asp Val Glu Arg Phe Phe Tyr Lys 
1               5                   10          


<210>  5
<211>  16
<212>  PRT
<213>  Staphylococcus aureus

<400>  5

His Thr Asp Asn Glu Leu Val Ser Ile Val Arg Arg Asp Leu Ser Gln 
1               5                   10                  15      


<210>  6
<211>  108
<212>  PRT
<213>  Staphylococcus aureus

<400>  6

Asn Lys Lys Ala His Ala Ile Phe Lys His Gly Met Thr Pro Ile Ile 
1               5                   10                  15      


Cys Val Gly Glu Thr Asp Glu Glu Arg Glu Ser Gly Lys Ala Asn Asp 
            20                  25                  30          


Val Val Gly Glu Gln Val Lys Lys Ala Val Ala Gly Leu Ser Glu Asp 
        35                  40                  45              


Gln Leu Lys Ser Val Val Ile Ala Tyr Glu Pro Ile Trp Ala Ile Gly 
    50                  55                  60                  


Thr Gly Lys Ser Ser Thr Ser Glu Asp Ala Asn Glu Met Cys Ala Phe 
65                  70                  75                  80  


Val Arg Gln Thr Ile Ala Asp Leu Ser Ser Lys Glu Val Ser Glu Ala 
                85                  90                  95      


Thr Arg Ile Gln Tyr Gly Gly Ser Val Lys Pro Asn 
            100                 105             


<210>  7
<211>  15
<212>  PRT
<213>  Staphylococcus aureus

<400>  7

Asn Lys Lys Ala His Ala Ile Phe Lys His Gly Met Thr Pro Ile 
1               5                   10                  15  


<210>  8
<211>  11
<212>  PRT
<213>  Staphylococcus aureus

<400>  8

Gln Leu Lys Ser Val Val Ile Ala Tyr Glu Pro 
1               5                   10      


<210>  9
<211>  15
<212>  PRT
<213>  Staphylococcus aureus

<400>  9

Ala Asn Glu Met Cys Ala Phe Val Arg Gln Thr Ile Ala Asp Leu 
1               5                   10                  15  


<210>  10
<211>  20
<212>  PRT
<213>  Staphylococcus aureus

<400>  10

Ser Ser Lys Glu Val Ser Glu Ala Thr Arg Ile Gln Tyr Gly Gly Ser 
1               5                   10                  15      


Val Lys Pro Asn 
            20  


<210>  11
<211>  130
<212>  PRT
<213>  artificial sequence

<220>
<223>  triepitope peptide sequence

<400>  11

Thr Asp Asn Glu Leu Val Ser Ile Val Arg Arg Asp Lys Ala His Ala 
1               5                   10                  15      


Ile Phe Lys His Gly Met Thr Pro Ile Ile Cys Val Gly Glu Thr Asp 
            20                  25                  30          


Glu Glu Arg Glu Ser Gly Lys Ala Asn Asp Val Val Gly Glu Gln Val 
        35                  40                  45              


Lys Lys Ala Val Ala Gly Leu Ser Glu Asp Gln Leu Lys Ser Val Val 
    50                  55                  60                  


Ile Ala Tyr Glu Pro Ile Trp Ala Ile Gly Thr Gly Lys Ser Ser Thr 
65                  70                  75                  80  


Ser Glu Asp Ala Asn Glu Met Cys Ala Phe Val Arg Gln Thr Ile Ala 
                85                  90                  95      


Asp Leu Ser Ser Lys Glu Val Ser Glu Ala Thr Arg Ile Gln Tyr Gly 
            100                 105                 110         


Gly Ser Val Lys Pro Asn Lys Asn Asp Gln Asp Val Glu Arg Phe Phe 
        115                 120                 125             


Tyr Lys 
    130 


<210>  12
<211>  118
<212>  PRT
<213>  artificial sequence

<220>
<223>  diepitope peptide sequence

<400>  12

Thr Asp Asn Glu Leu Val Ser Ile Val Arg Arg Asp Lys Ala His Ala 
1               5                   10                  15      


Ile Phe Lys His Gly Met Thr Pro Ile Ile Cys Val Gly Glu Thr Asp 
            20                  25                  30          


Glu Glu Arg Glu Ser Gly Lys Ala Asn Asp Val Val Gly Glu Gln Val 
        35                  40                  45              


Lys Lys Ala Val Ala Gly Leu Ser Glu Asp Gln Leu Lys Ser Val Val 
    50                  55                  60                  


Ile Ala Tyr Glu Pro Ile Trp Ala Ile Gly Thr Gly Lys Ser Ser Thr 
65                  70                  75                  80  


Ser Glu Asp Ala Asn Glu Met Cys Ala Phe Val Arg Gln Thr Ile Ala 
                85                  90                  95      


Asp Leu Ser Ser Lys Glu Val Ser Glu Ala Thr Arg Ile Gln Tyr Gly 
            100                 105                 110         


Gly Ser Val Lys Pro Asn 
        115             


<210>  13
<211>  130
<212>  PRT
<213>  artificial sequence

<220>
<223>  diepitope peptide sequence

<400>  13

Thr Asp Asn Glu Leu Val Ser Ile Val Arg Arg Asp Lys Ala His Ala 
1               5                   10                  15      


Ile Phe Lys His Gly Met Thr Pro Ile Ile Cys Val Gly Glu Thr Asp 
            20                  25                  30          


Glu Glu Arg Glu Ser Gly Lys Ala Asn Asp Val Val Gly Glu Gln Val 
        35                  40                  45              


Lys Lys Ala Val Ala Gly Leu Ser Glu Asp Gln Leu Lys Ser Val Val 
    50                  55                  60                  


Ile Ala Tyr Glu Pro Ile Trp Ala Ile Gly Thr Gly Lys Ser Ser Thr 
65                  70                  75                  80  


Ser Glu Asp Ala Asn Glu Met Cys Ala Phe Val Arg Gln Thr Ile Ala 
                85                  90                  95      


Asp Leu Ser Ser Lys Glu Val Ser Glu Ala Thr Arg Ile Gln Tyr Gly 
            100                 105                 110         


Gly Ser Val Lys Pro Asn Thr Asp Asn Glu Leu Val Ser Ile Val Arg 
        115                 120                 125             


Arg Asp 
    130 


<210>  14
<211>  118
<212>  PRT
<213>  artificial sequence

<220>
<223>  diepitope peptide sequence

<400>  14

Lys Ala His Ala Ile Phe Lys His Gly Met Thr Pro Ile Ile Cys Val 
1               5                   10                  15      


Gly Glu Thr Asp Glu Glu Arg Glu Ser Gly Lys Ala Asn Asp Val Val 
            20                  25                  30          


Gly Glu Gln Val Lys Lys Ala Val Ala Gly Leu Ser Glu Asp Gln Leu 
        35                  40                  45              


Lys Ser Val Val Ile Ala Tyr Glu Pro Ile Trp Ala Ile Gly Thr Gly 
    50                  55                  60                  


Lys Ser Ser Thr Ser Glu Asp Ala Asn Glu Met Cys Ala Phe Val Arg 
65                  70                  75                  80  


Gln Thr Ile Ala Asp Leu Ser Ser Lys Glu Val Ser Glu Ala Thr Arg 
                85                  90                  95      


Ile Gln Tyr Gly Gly Ser Val Lys Pro Asn Thr Asp Asn Glu Leu Val 
            100                 105                 110         


Ser Ile Val Arg Arg Asp 
        115             


<210>  15
<211>  10
<212>  PRT
<213>  Mus musculus

<400>  15

Gly Tyr Thr Phe Thr Asp Tyr Ser Met His 
1               5                   10  


<210>  16
<211>  17
<212>  PRT
<213>  Mus musculus

<400>  16

Trp Ile Asp Thr Glu Thr Gly Glu Pro Thr Phe Ala Asp Asp Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  17
<211>  11
<212>  PRT
<213>  Mus musculus

<400>  17

Pro Leu Leu Arg Leu Ser Phe Ala Met Asp Ser 
1               5                   10      


<210>  18
<211>  15
<212>  PRT
<213>  Mus musculus

<400>  18

Ser Ala Ser Glu Asn Leu Asp Ile Tyr Gly Asn Ser Phe Met His 
1               5                   10                  15  


<210>  19
<211>  7
<212>  PRT
<213>  Mus musculus

<400>  19

Arg Ala Ser Lys Leu Glu Ser 
1               5           


<210>  20
<211>  9
<212>  PRT
<213>  Mus musculus

<400>  20

Gln Gln Thr Ser Glu Asp Pro Arg Thr 
1               5                   


<210>  21
<211>  6
<212>  PRT
<213>  Mus musculus

<400>  21

Ser Arg Tyr Trp Ile Glu 
1               5       


<210>  22
<211>  17
<212>  PRT
<213>  Mus musculus

<400>  22

Glu Ile Leu Pro Gly Ser Gly Ser Thr Asn Tyr Asn Glu Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  23
<211>  12
<212>  PRT
<213>  Mus musculus

<400>  23

Phe Tyr Ser Gly Asn Phe Val Gly Pro Val Asp Tyr 
1               5                   10          


<210>  24
<211>  16
<212>  PRT
<213>  Mus musculus

<400>  24

Lys Ser Ser Gln Ser Leu Ser Asp Ser Asp Gly Lys Thr Tyr Leu Asn 
1               5                   10                  15      


<210>  25
<211>  7
<212>  PRT
<213>  Mus musculus

<400>  25

Leu Val Ser Lys Val Asp Ser 
1               5           


<210>  26
<211>  9
<212>  PRT
<213>  Mus musculus

<400>  26

Trp Gln Gly Thr His Phe Pro Phe Thr 
1               5                   


<210>  27
<211>  10
<212>  PRT
<213>  Mus musculus

<400>  27

Gly Phe Thr Phe Ser Ala Tyr Trp Met Asn 
1               5                   10  


<210>  28
<211>  19
<212>  PRT
<213>  Mus musculus

<400>  28

Glu Ile Arg Met Lys Ser Asn Asn Tyr Ala Thr His Tyr Ala Glu Ser 
1               5                   10                  15      


Val Lys Gly 
            


<210>  29
<211>  7
<212>  PRT
<213>  Mus musculus

<400>  29

Tyr Lys Tyr Asp Gly Ala Tyr 
1               5           


<210>  30
<211>  10
<212>  PRT
<213>  Mus musculus

<400>  30

Ser Ala Ser Ser Thr Val Asn Tyr Met His 
1               5                   10  


<210>  31
<211>  7
<212>  PRT
<213>  Mus musculus

<400>  31

Asp Thr Ser Lys Leu Ala Ser 
1               5           


<210>  32
<211>  8
<212>  PRT
<213>  Mus musculus

<400>  32

His Gln Arg Ser Ser Tyr Pro Thr 
1               5               


- 2 -

