                         SEQUENCE LISTING

<110>  Hyasynth Biologicals Inc.
 
<120>  METHOD AND CELL LINE FOR PRODUCTION OF POLYKETIDES IN 
YEAST

<130>  PAT 85146W-90

<150>  US 62/460,526
<151>  2017-02-17

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  9444
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Yeast optimized DiPKS from Dictyostelium discoideum


<220>
<221>  Motif 1
<222>  (4528)..(4554)

<220>
<221>  C-methyltransferase domain
<222>  (4528)..(4890)

<220>
<221>  Motif 2
<222>  (4787)..(4809)

<220>
<221>  Motif 3
<222>  (4867)..(4899)

<400>  1
atgaacaaga actccaaaat ccagtcccca aactcttctg atgttgctgt tattggtgtt       60

ggttttagat tcccaggtaa ctctaatgac ccagaatctt tgtggaacaa cttgttggat      120

ggtttcgatg ctattaccca agtcccaaaa gaaagatggg ctacttcttt tagagagatg      180

ggtttgatca agaacaagtt cggtggtttc ttgaaggatt ctgaatggaa gaatttcgac      240

cctttgttct ttggtatcgg tccaaaagaa gctccattca ttgatccaca acaaaggttg      300

ttgttgtcca tcgtttggga atctttggaa gatgcttaca tcagaccaga tgaattgaga      360

ggttctaaca ctggtgtttt catcggtgtt tctaacaacg attacaccaa gttgggtttc      420

caagacaact actctatttc tccatacact atgaccggct ctaactcttc attgaactcc      480

aacagaattt cctactgctt cgattttaga ggtccatcca ttactgttga taccgcttgt      540

tcttcttcct tggtttctgt taatttgggt gtccaatcca tccaaatggg tgaatgtaag      600

attgctattt gcggtggtgt taacgctttg tttgatccat ctacatctgt tgccttttcc      660

aagttgggtg ttttgtctga aaatggcaga tgcaactctt ttagtgatca agcctctggt      720

tacgttagat ctgaaggtgc tggtgttgtt gttttgaagt ctttggaaca agctaagttg      780

gatggtgata gaatctacgg tgttatcaag ggtgtttcct ctaatgaaga tggtgcttct      840

aatggtgaca agaactcttt gactactcca tcttgtgaag cccaatccat taacatttct      900

aaggctatgg aaaaggcctc cttgtctcca tctgatatct attacattga agcccatggt      960

actggtactc cagttggtga tccaattgaa gttaaggcct tgtccaagat cttctccaac     1020

tctaacaaca accagttgaa caacttctct accgatggta atgataacga tgatgatgat     1080

gacgataaca cctctccaga accattattg attggctcat tcaagtccaa catcggtcat     1140

ttggaatctg ctgctggtat tgcttctttg attaagtgtt gcttgatgtt gaagaacagg     1200

atgttggttc catccattaa ctgctctaat ttgaacccat ccattccatt cgatcagtac     1260

aacatctccg ttatcagaga aatcagacaa ttcccaaccg ataagttggt taacatcggt     1320

atcaattctt tcggtttcgg tggttctaac tgccatttga ttattcaaga gtacaacaac     1380

aacttcaaga acaactctac catctgcaat aacaacaaca acaacaataa caacatcgac     1440

tacttgatcc caatctcctc taagactaag aagtccttgg ataagtactt gattttgatc     1500

aagaccaact ccaactacca caaggatatt tctttcgatg acttcgtcaa gttccaaatc     1560

aagtctaagc agtacaactt gtccaacaga atgactacca ttgctaacga ttggaactcc     1620

ttcattaagg gttctaacga attccacaac ttgatcgaat ctaaggatgg tgaaggtggt     1680

tcttcatctt ctaacagagg tattgattcc gccaatcaaa tcaacactac tactacctct     1740

accatcaacg atatcgaacc tttgttggtt ttcgttttct gtggtcaagg tccacaatgg     1800

aatggtatga ttaagacctt gtacaactcc gagaacgttt tcaagaacac cgttgatcat     1860

gttgacagca tcttgtacaa gtacttcggt tactccattt tgaacgtctt gtctaagatc     1920

gatgataacg acgattccat caaccatcca atagttgctc aaccatcttt gttcttgttg     1980

caaattggtt tggtcgagtt gtttaagtac tggggtatct acccatctat ctctgttggt     2040

cattctttcg gtgaagtctc ttcttattac ttgtccggta tcatctcttt ggaaaccgct     2100

tgtaaaatcg tctacgtcag atcctctaat cagaacaaaa ctatgggttc cggtaagatg     2160

ttggttgttt ctatgggttt taagcaatgg aacgatcaat tctctgctga atggtccgat     2220

attgaaattg cttgttacaa cgctccagat tccatagttg ttactggtaa cgaagaaaga     2280

ttgaaagaat tgtccatcaa gttgtccgac gaatccaatc aaattttcaa caccttcttg     2340

aggtccccat gttcttttca ttcttcccat caagaagtca tcaagggttc tatgttcgaa     2400

gagttgtcta acttgcaatc tactggtgaa accgaaatcc ctttgttctc tactgttact     2460

ggtagacaag ttttgtctgg tcatgttact gctcaacaca tctacgataa tgttagagaa     2520

ccagtcttgt tccaaaagac gattgaatcc attacctcct acatcaagtc tcactaccca     2580

tccaatcaaa aggttatcta cgttgaaatt gctccacacc caaccttgtt ttcattgatc     2640

aaaaagtcca tcccatcctc caacaagaat tcctcttctg ttttgtgtcc attgaacaga     2700

aaagaaaact ccaacaactc ctacaagaag ttcgtttctc agttgtactt caacggtgtt     2760

aacgttgact tcaacttcca gttgaactcc atttgcgata acgttaacaa cgatcaccat     2820

ttgaacaacg tcaagcaaaa ctccttcaaa gagactacca attccttgcc aagataccaa     2880

tgggaacaag atgaatattg gtccgaacca ttgatctcca gaaagaatag attggaaggt     2940

ccaactactt ccttgttggg tcatagaatt atctacagct tcccagtttt ccaatccgtt     3000

ttggacttgc aatctgacaa ctacaaatac ttgttggacc acttggttaa cggtaagcca     3060

gtttttccag gtgctggtta tttggatatc atcatcgaat tcttcgacta ccaaaagcag     3120

cagttgaatt cctctgattc ctctaactcc tacatcatca acgttgacaa gatccaattc     3180

ttgaacccaa ttcacttgac cgaaaacaag ttgcaaacct tgcaatcttc tttcgaacct     3240

atcgttacta agaagtctgc cttctctgtt aacttcttca tcaaggatac cgtcgaggat     3300

caatctaagg ttaagtctat gtctgacgaa acttggacta acacttgtaa ggctaccatt     3360

tccttggaac aacaacagcc atctccatct tctactttga ctttgtctaa gaagcaagac     3420

ttgcagatct tgagaaacag atgcgatatt agcaagctag acaagtttga gttgtacgac     3480

aagatctcta agaatttggg cttgcagtac aactccttgt ttcaagttgt tgataccatc     3540

gaaactggta aggattgctc ttttgctact ttgtctttgc cagaagatac tttgttcacc     3600

accattttga acccatgctt gttggataac tgtttccatg gtttgttgac cttgatcaac     3660

gaaaagggtt ctttcgttgt cgagtccatt tcttctgttt ctatctactt ggagaacatc     3720

ggttccttca atcaaacttc tgttggtaac gtccagttct acttgtacac cactatttct     3780

aaagccacct cctttagttc tgaaggtact tgtaagttgt tcaccaagga tggttccttg     3840

attttgtcta tcggtaagtt catcatcaag tccaccaatc caaagtctac taagaccaac     3900

gaaactatcg aatctccatt ggacgaaacc ttctctattg aatggcaatc taaggattct     3960

ccaattccaa ccccacaaca aatccaacaa caatctccat tgaactctaa cccatccttc     4020

attagatcta ccatcttgaa ggacatccag ttcgaacaat actgctcctc cattatccac     4080

aaagaattga tcaaccacga aaagtacaag aaccagcaat ccttcgatat caactccttg     4140

gaaaaccact tgaacgatga ccaattgatg gaatccttgt ccatctccaa agaatacttg     4200

agattcttca ccaggatcat ctccatcatt aagcaatacc caaagatctt gaacgaaaaa     4260

gagctaaaag aattgaaaga aatcatcgaa ttgaagtacc catccgaagt tcagttgttg     4320

gaattcgaag ttatcgagaa ggtgtccatg attatcccaa agttgttgtt cgaaaacgac     4380

aagcaatctt ccatgacctt gttccaagat aacttgttga ccaggttcta ctccaattct     4440

aactctacca gattctactt ggaaagggtt tccgaaatgg tcttggaatc tattagacca     4500

atcgtcagag aaaagagggt gttcagaatt ttggaaattg gtgctggtac aggctctttg     4560

tctaatgttg ttttgactaa gttgaacacc tacttgtcca ccttgaattc taatggtggt     4620

tctggttaca acatcatcat tgagtacacc ttcaccgata tttccgccaa cttcattatt     4680

ggtgaaatcc aagaaaccat gtgcaacttg tacccaaacg ttactttcaa gttctccgtc     4740

ttggacttgg agaaagagat tattaactcc tccgatttct tgatgggtga ttacgatata     4800

gttttgatgg cctacgttat ccatgccgtt tctaacatta agttctccat cgaacagttg     4860

tacaagttgt tgtctccaag aggttggttg ttgtgtattg aacctaagtc caacgttgtg     4920

ttctccgatt tggttttcgg ttgttttaat cagtggtgga actactacga tgatattaga     4980

actacccact gctccttgtc tgaatctcaa tggaatcagt tgttgttgaa ccagtccttg     5040

aacaacgaat cctcttcttc ttctaactgt tacggtggtt tctccaacgt ttcttttatt     5100

ggtggtgaaa aggatgtcga ctcccattct ttcatattgc actgccaaaa agaatccatc     5160

tcccaaatga agttagccac cactattaac aacggtttgt catctggttc catcgttatc     5220

gttttgaact ctcaacaatt gaccaacatg aagtcctacc caaaggttat tgagtatatt     5280

caagaggcta cctctttgtg caagaccatt gaaattatcg attccaagga cgtcttgaac     5340

tctaccaatt cagttttgga aaagatccaa aagtccttgt tggtgttctg tttgttgggt     5400

tatgacttgt tggagaacaa ctaccaagaa cagtctttcg aatacgttaa gttgttgaac     5460

ttgatctcta ctaccgcctc ttcatctaat gataagaaac caccaaaggt cttgttgatc     5520

accaagcaat ctgaaagaat ctccaggtct ttctactcca gatccttgat tggtatttcc     5580

agaacctcta tgaacgagta cccaaatttg tccattacct ctatcgattt ggataccaac     5640

gactactcat tgcagtcttt gttgaagcca atcttcagca actctaagtt ttccgacaac     5700

gagttcatct tcaaaaaggg cttgatgttc gtgtccagga tctttaagaa caagcagttg     5760

ctagaatcct ccaacgcttt tgaaactgac tcttctaact tgtactgtaa ggcctcttct     5820

gacttgtctt acaagtacgc tattaagcag tctatgttga ccgaaaatca gatcgaaatc     5880

aaggttgaat gcgtcggtat taacttcaag gacaacctat tctacaaggg cttgttgcca     5940

caagaaattt tcagaatggg tgacatctac aatccaccat atggtttgga atgctctggt     6000

gttattacca gaattggttc taacgtcacc gaatactcag ttggtcaaaa tgtttttggt     6060

ttcgccagac attctttggg ttctcatgtt gttaccaaca aggatttggt tatcttgaag     6120

ccagatacca tctcattttc tgaagctgct tctatcccag ttgtttactg tactgcttgg     6180

tactccttgt tcaacattgg tcagttgtct aacgaagaat ccatcctaat tcattctgct     6240

actggtggtg taggtttggc ttctttgaat ttgttgaaaa tgaagaatca gcaacagcaa     6300

ccattgacca atgtttatgc tactgttggc tctaacgaga agaagaagtt cttgatcgat     6360

aacttcaaca acttgttcaa agaggacggc gaaaacattt tctctaccag agacaaagaa     6420

tactccaacc agttggaatc caagatcgat gttattttga acaccttgtc cggtgaattc     6480

gtcgaatcta atttcaagtc cttgagatcc ttcggtagat tgattgattt gtctgctact     6540

cacgtttacg ccaatcaaca aattggtcta ggtaacttca agttcgacca cttgtattct     6600

gctgttgact tggaaagatt gatcgacgaa aaacctaagt tgttgcagtc catcttgcaa     6660

agaattacca actctatcgt caacggttcc ttggaaaaaa ttccaattac catcttccca     6720

tccaccgaaa ctaaggatgc tatcgaatta ttgtccaaga gatcccatat cggtaaagtt     6780

gttgtagatt gcaccgatat ctctaagtgt aatcctgttg gtgatgtgat caccaacttc     6840

tctatgagat tgccaaagcc aaactaccag ttgaatttga actccacctt gttgattact     6900

ggtcagtctg gtttgtctat ccctttgttg aattggttgt tgtctaagtc tggtggtaac     6960

gttaagaacg ttgtcatcat ttctaagtcc accatgaagt ggaagttgca gactatgatt     7020

tcccatttcg tttccggttt cggtatccat tttaactacg ttcaagtcga catctccaac     7080

tacgatgctt tgtctgaagc tattaagcaa ttgccatctg atttgccacc aatcacctct     7140

gtttttcatt tggctgctat ctacaacgat gttccaatgg atcaagttac catgtctacc     7200

gttgaatctg ttcataaccc taaagttttg ggtgccgtta acttgcatag aatctctgtt     7260

tcttttggtt ggaagttgaa ccacttcgtc ttgttctctt ctattactgc tattaccggt     7320

tacccagacc aatctatcta caattctgcc aactctattt tggacgcttt gtccaacttt     7380

agaaggttta tgggtttgcc atccttctcc attaacttgg gtccaatgaa ggatgaaggt     7440

aaggtttcta ccaacaagag catcaagaag ctattcaagt ctagaggttt gccaagccta     7500

tccttgaaca agttatttgg tttgttggag gtcgtcatca acaacccatc taatcatgtt     7560

atcccatccc aattgatttg ctccccaatc gatttcaaga cctacatcga atctttctca     7620

actatgaggc caaagttgtt acacttgcaa cctaccattt ccaagcagca atcttctatc     7680

attaacgatt ctaccaaggc ttcctccaac atttcattgc aagataagat cacctccaag     7740

gtgtctgatt tgttgtccat tccaatctcc aagatcaact tcgatcatcc attgaaacac     7800

tacggcttgg attctttgtt gaccgttcaa ttcaaatcct ggatcgacaa agaattcgaa     7860

aagaacttgt tcacccatat ccaattggcc accatctcta ttaactcatt cttggaaaag     7920

gtgaacggct tgtctacaaa caataacaac aacaacaatt ccaacgtcaa gtcctctcca     7980

tccattgtca aagaagaaat cgttaccttg gacaaggatc aacaaccatt gctattgaaa     8040

gaacaccagc acattatcat ctccccagat attagaatca acaagccaaa gagggaatcc     8100

ttgattagaa ccccaatctt gaacaaattc aaccagatca ccgaatccat tatcactcca     8160

tctacaccat ctttgtccca atccgatgtt ttgaaaactc caccaatcaa gtctttgaac     8220

aacactaaga actccagctt gattaacacc ccaccaattc aatctgtcca acaacatcaa     8280

aagcaacaac aaaaggtcca agtcatccaa caacagcaac aaccattatc cagattgtcc     8340

tacaagagca acaacaactc tttcgttttg ggtatcggta tttctgttcc aggtgaacct     8400

atttcccaac aatccttgaa agactccatc tccaatgact tttctgataa ggctgaaact     8460

aacgagaagg tcaagagaat ctttgagcaa tctcaaatca agaccagaca cttggttaga     8520

gattacacta agccagagaa ctccatcaag ttcagacatt tggaaaccat taccgatgtg     8580

aacaaccagt tcaagaaagt tgttccagat ttggctcaac aagcctgttt gagagctttg     8640

aaagattggg gtggtgataa gggtgatatt acccatatag tttctgttac ctccaccggt     8700

attatcatcc cagatgttaa tttcaagttg atcgacttgt tgggcttgaa caaggatgtt     8760

gaaagagtgt ctttgaacct aatgggttgt ttggctggtt tgagttcttt gagaactgct     8820

gcttctttgg ctaaggcttc tccaagaaat agaattttgg ttgtctgtac cgaagtctgc     8880

tccttgcatt tttctaatac tgatggtggt gatcaaatgg tcgcctcttc tatttttgct     8940

gatggttctg ctgcttacat tattggttgt aacccaagaa ttgaagaaac cccattatac     9000

gaagtcatgt gctccattaa cagatctttc ccaaataccg aaaacgccat ggtttgggat     9060

ttggaaaaag aaggttggaa cttgggtttg gatgcttcta ttccaattgt cattggttct     9120

ggtattgaag ccttcgttga tactttgttg gataaggcta agttgcaaac ttccactgct     9180

atttctgcta aggattgcga attcttgatt catactggtg gcaagtccat cttgatgaac     9240

atcgaaaatt ccttgggtat cgacccaaag caaactaaga atacttggga tgtttaccat     9300

gcctacggca atatgtcatc tgcctctgtt attttcgtta tggatcatgc cagaaagtcc     9360

aagtctttgc caacttactc aatttctttg gcttttggtc caggtttggc ttttgaaggt     9420

tgtttcttga agaacgtcgt ctaa                                            9444


<210>  2
<211>  14025
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid


<220>
<221>  C1:p506 primer homology
<222>  (1)..(50)

<220>
<221>  19 UP
<222>  (51)..(761)

<220>
<221>  L0
<222>  (762)..(800)

<220>
<221>  THD3p
<222>  (801)..(1453)

<220>
<221>  L1
<222>  (1454)..(1493)

<220>
<221>  ALD6
<222>  (1494)..(2999)

<220>
<221>  L2
<222>  (3000)..(3039)

<220>
<221>  LTP1
<222>  (3364)..(3403)

<220>
<221>  Tef1p
<222>  (3404)..(3897)

<220>
<221>  L3
<222>  (3898)..(3937)

<220>
<221>  Acs L641P
<222>  (3938)..(5893)

<220>
<221>  L4
<222>  (5894)..(5933)

<220>
<221>  PRM9t
<222>  (5934)..(6471)

<220>
<221>  LTP2
<222>  (6472)..(6511)

<400>  2
taaccctcac taaagggaac aaaagctgga gctcgtttaa acggcgcgcc caccggagct       60

tggatatgat aaacgaaata ttcttgaatc gtgagatcgc ctgttttcaa aaccgttgga      120

ggcagaaaca attttgtcac aagatgggca ttctacccca tccttgctgt attattgtag      180

tctcgctttc ttttatgctg gacaaatgag actactgcac atttttatac gttcttggtt      240

ttttttaaag gtgtggtttc ggcattatcc tgccgcacgt ttcttggata attcatcctg      300

attctctatt ttaaacgctt cagcctatca ggatttggtt ttgatacata ctgcaagagt      360

gtatctcggg aacagtcatt tattccgcaa caaacttaat tgcggaacgc gttaggcgat      420

ttctagcata tatcaaatac cgttcgcgat ttcttctggg ttcgtctctt ttcttttaaa      480

tacttattaa cgtactcaaa caactacact tcgttgtatc tcagaatgag atccctcagt      540

atgacaatac atcattctaa acgttcgtaa aacacatatg aaacaacttt ataacaaagc      600

gaacaaaatg ggcaacatga gatgaaactc cgcgtccctt agctgaacta cccaaacgta      660

cgaatgcctg aacaattagt ttagatccga gattccgcgc ttccatcatt tagtataatc      720

catattttat ataatatata ggataagtaa cagcccgcga aaaacaacaa ataatcataa      780

aaattttaga actagacata tcgagtttat cattatcaat actgccattt caaagaatac      840

gtaaataatt aatagtagtg attttcctaa ctttatttag tcaaaaaatt agccttttaa      900

ttctgctgta acccgtacat gcccaaaata gggggcgggt tacacagaat atataacatc      960

gtaggtgtct gggtgaacag tttattcctg gcatccacta aatataatgg agcccgcttt     1020

ttaagctggc atccagaaaa aaaaagaatc ccagcaccaa aatattgttt tcttcaccaa     1080

ccatcagttc ataggtccat tctcttagcg caactacaga gaacaggggc acaaacaggc     1140

aaaaaacggg cacaacctca atggagtgat gcaacctgcc tggagtaaat gatgacacaa     1200

ggcaattgac ccacgcatgt atctatctca ttttcttaca ccttctatta ccttctgctc     1260

tctctgattt ggaaaaagct gaaaaaaaag gttgaaacca gttccctgaa attattcccc     1320

tacttgacta ataagtatat aaagacggta ggtattgatt gtaattctgt aaatctattt     1380

cttaaacttc ttaaattcta cttttatagt tagtcttttt tttagtttta aaacaccaag     1440

aacttagttt cgactagaaa atttattata aaaggaagag aaataattaa acaatgacta     1500

agctacactt tgacactgct gaaccagtca agatcacact tccaaatggt ttgacatacg     1560

agcaaccaac cggtctattc attaacaaca agtttatgaa agctcaagac ggtaagacct     1620

atcccgtcga agatccttcc actgaaaaca ccgtttgtga ggtctcttct gccaccactg     1680

aagatgttga atatgctatc gaatgtgccg accgtgcttt ccacgacact gaatgggcta     1740

cccaagaccc aagagaaaga ggccgtctac taagtaagtt ggctgacgaa ttggaaagcc     1800

aaattgactt ggtttcttcc attgaagctt tggacaatgg taaaactttg gccttagccc     1860

gtggggatgt taccattgca atcaactgtc taagagatgc tgctgcctat gccgacaaag     1920

tcaacggtag aacaatcaac accggtgacg gctacatgaa cttcaccacc ttagagccaa     1980

tcggtgtctg tggtcaaatt attccatgga actttccaat aatgatgttg gcttggaaga     2040

tcgccccagc attggccatg ggtaacgtct gtatcttgaa acccgctgct gtcacacctt     2100

taaatgccct atactttgct tctttatgta agaaggttgg tattccagct ggtgtcgtca     2160

acatcgttcc aggtcctggt agaactgttg gtgctgcttt gaccaacgac ccaagaatca     2220

gaaagctggc ttttaccggt tctacagaag tcggtaagag tgttgctgtc gactcttctg     2280

aatctaactt gaagaaaatc actttggaac taggtggtaa gtccgcccat ttggtctttg     2340

acgatgctaa cattaagaag actttaccaa atctagtaaa cggtattttc aagaacgctg     2400

gtcaaatttg ttcctctggt tctagaattt acgttcaaga aggtatttac gacgaactat     2460

tggctgcttt caaggcttac ttggaaaccg aaatcaaagt tggtaatcca tttgacaagg     2520

ctaacttcca aggtgctatc actaaccgtc aacaattcga cacaattatg aactacatcg     2580

atatcggtaa gaaagaaggc gccaagatct taactggtgg cgaaaaagtt ggtgacaagg     2640

gttacttcat cagaccaacc gttttctacg atgttaatga agacatgaga attgttaagg     2700

aagaaatttt tggaccagtt gtcactgtcg caaagttcaa gactttagaa gaaggtgtcg     2760

aaatggctaa cagctctgaa ttcggtctag gttctatggg tatcgaaaca gaatctttga     2820

gcacaggttt gaaggtggcc aagatgttga aggccggtac cgtctggatc aacacataca     2880

acgattttga ctccagagtt ccattcggtg gtgttaagca atctggttac ggtagagaaa     2940

tgggtgaaga agtctaccat gcatacactg aagtaaaagc tgtcagaatt aagttgtaaa     3000

gacataaaac tgaaacaaca ccaattaata atagactttt ggacttcttc gccagaggtt     3060

tggtcaagtc tccaatcaag gttgtcggct tgtctacctt gccagaaatt tacgaaaaga     3120

tggaaaaggg tcaaatcgtt ggtagatacg ttgttgacac ttctaaataa gcgaatttct     3180

tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt     3240

aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg     3300

tcaggttgct ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca     3360

tggcttaaat aacatactca tcactaaaca ttcttaacaa tcaaagcaac aggcgcgttg     3420

gacttttaat tttcgaggac cgcgaatcct tacatcacac ccaatccccc acaagtgatc     3480

ccccacacac catagcttca aaatgtttct actccttttt tactcttcca gattttctcg     3540

gactccgcgc atcgccgtac cacttcaaaa cacccaagca cagcatacta aatttcccct     3600

ctttcttcct ctagggtgtc gttaattacc cgtactaaag gtttggaaaa gaaaaaagag     3660

accgcctcgt ttctttttct tcgtcgaaaa aggcaataaa aatttttatc acgtttcttt     3720

ttcttgaaaa tttttttttt tgattttttt ctctttcgat gacctcccat tgatatttaa     3780

gttaataaac ggtcttcaat ttctcaagtt tcagtttcat ttttcttgtt ctattacaac     3840

tttttttact tcttgctcat tagaaagaaa gcatagcaat ctaatctaag ttttaataca     3900

tctaccagtc aacagccaac aattaactaa ttaaacaatg tcccaaactc ataagcacgc     3960

tattccagct aatattgctg atagatgctt gatcaaccca gaacagtacg aaactaagta     4020

caagcaatcc atcaacgatc cagatacttt ttggggtgaa caaggtaaga ttttggattg     4080

gattacccca taccaaaagg tcaagaatac ttcttttgct ccaggcaacg tttccattaa     4140

gtggtatgaa gatggtactt tgaacttggc tgctaactgt ttggatagac acttgcaaga     4200

aaacggtgat agaaccgcta ttatttggga aggtgatgat acctcccaat ccaaacatat     4260

ctcttacaga gaattgcaca gagatgtctg tagattcgct aacactttgt tggatttggg     4320

catcaaaaag ggtgatgttg ttgctatcta tatgccaatg gttcctgaag ctgctgttgc     4380

tatgttggct tgtgctagaa ttggtgctgt tcattctgtt attttcggtg gtttttcacc     4440

agaagctgtt gccggtagaa ttatcgattc ttcatccaga ttggttatca ccgctgatga     4500

aggtgttaga gctggtagat ctattccatt gaaaaagaac gttgatgacg ccttgaagaa     4560

cccaaatgtt acttctgttg aacacgtcat cgttttgaag agaactggtt ctgatatcga     4620

ttggcaagag ggtagagatt tgtggtggag agatttgatt gaaaaggctt ctccagaaca     4680

tcaaccagaa gctatgaacg ctgaagatcc tttgtttatc ttgtacactt ctggttctac     4740

tggtaagcca aaaggtgttt tacacactac tggtggttat ttggtttacg ctgctactac     4800

tttcaagtac gttttcgatt atcacccagg tgatatctat tggtgtactg ctgatgttgg     4860

ttgggttact ggtcattctt atttgttgta tggtccattg gcttgtggtg ctactacatt     4920

gatgtttgaa ggtgttccaa attggccaac tccagctaga atgtgtcaag ttgttgacaa     4980

acaccaagtc aacatcttgt atactgctcc aactgctatt agagctttga tggctgaagg     5040

tgataaggct attgaaggta ctgatagatc ctccttgaga atcttgggtt ctgttggtga     5100

acctattaac cctgaagcct gggaatggta ttggaagaaa attggtaaag aaaagtgccc     5160

agttgttgat acttggtggc aaactgaaac tggtggtttt atgattactc cattgccagg     5220

tgctattgaa ttgaaagctg gttctgctac tagaccattt tttggtgttc aaccagcttt     5280

ggttgataac gaaggtcatc cacaagaagg tgctactgaa ggtaatttgg ttattactga     5340

ttcttggcca ggtcaagcta gaactttgtt tggtgatcac gaaagattcg aacagactta     5400

cttctctacc ttcaagaaca tgtacttctc tggtgatggt gctagaagag atgaagatgg     5460

ttactattgg attaccggta gagttgatga tgtcttgaat gtttctggtc acagattagg     5520

tactgccgaa attgaatctg ctttggttgc tcatccaaag attgctgaag ctgcagttgt     5580

tggtattcca catgctatta agggtcaagc tatctacgct tacgttactt tgaatcatgg     5640

tgaagaacca tctccagaat tatacgctga agttagaaac tgggtcagaa aagaaattgg     5700

tccattagct accccagatg ttttacattg gactgattct ttgccaaaga ccagatcagg     5760

taagatcatg agaagaatct tgagaaagat tgctgctggt gatacttcta acttgggtga     5820

tacttcaaca ttagctgatc caggtgttgt tgaaaagcct ttggaagaaa aacaagctat     5880

tgccatgcca tcctaataat taaatactat tttcaaaatt ctacttaaaa ataacagaag     5940

acgggagaca ctagcacaca actttaccag gcaaggtatt tgacgctagc atgtgtccaa     6000

ttcagtgtca tttatgattt tttgtagtag gatataaata tatacagcgc tccaaatagt     6060

gcggttgccc caaaaacacc acggaacctc atctgttctc gtactttgtt gtgacaaagt     6120

agctcactgc cttattatca cattttcatt atgcaacgct tcggaaaata cgatgttgaa     6180

aatgcctcta gagatgaaaa acaatcgtaa aagggtcctg cgtaattgaa acatttgatc     6240

agtatgcagt ggcacagaaa caaccaggaa tactatagtc ataggcaata caaggtatat     6300

attggctatg cagacccctc cagaaagtac cgacgtcaag ttagatacac ttaacgaacc     6360

tagtgcacat ttaattgaga aaaatgtggc tcttcctaag gacatattcc gttcgtactt     6420

gagttattgg atctatgaaa tcgctcgcta tacaccagtc atgattttgt cattgcgaag     6480

actatactga tatatgaatt taaactagag cggaccaact atcatccgct aattactgac     6540

attaccaaat gagatctgtg aatgggcaag ataaaaaaca aaaattgaaa tgtttgacgt     6600

tatgtaaaac tattaattcc ttcgctttcg gcggtcacag aatttgcgtg tagctgactc     6660

ttgttcaatc aatatcattt gttactttat ttgaaagtct gtattactgc gcctattgtc     6720

atccgtacca aagaacgtca aaaagaaaca agataatttt tgtgcttaca ccatttatag     6780

atcactgagc ccagaatatc gctggagctc agtgtaagtg gcatgaacac aactctgact     6840

gatcgcacat attgccgtta tcataaatac tagttgtact tgtcaatgcg acgaatggca     6900

tcatgcctat tattacgttc ctctttttcc gtttcatgtt tccagaatgc tattgaatct     6960

aacacttcaa ttataaaaaa gaataaatcc gcaataattt taggctaatt gttgtactgt     7020

caagcgaacc taatggttaa aattcagagg aaccttcgac gtagtctgat cgctacttct     7080

atatcttatg ttcccagtca atcaaaagtt gatactataa tagctgccat ttatacctgt     7140

tagttatggc gatcgtttat cacggcggcc gcggtaccta ataacttcgt atagcataca     7200

ttatacgaag ttatattaag ggttctcgac gttttcgaca ctggatggcg gcgttagtat     7260

cgaatcgaca gcagtatagc gaccagcatt cacatacgat tgacgcatga tattactttc     7320

tgcgcactta acttcgcatc tgggcagatg atgtcgaggc gaaaaaaaat ataaatcacg     7380

ctaacatttg attaaaatag aacaactaca atataaaaaa actatacaaa tgacaagttc     7440

ttgaaaacaa gaatcttttt attgtcagta ctgattagaa aaactcatcg agcatcaaat     7500

gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct     7560

gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt     7620

ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa     7680

ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct     7740

tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac     7800

tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat     7860

cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca     7920

gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt     7980

tgccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga     8040

tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat     8100

cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat     8160

acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat     8220

ataaatcagc atccatgttg gaatttaatc gcggcctcga aacgtgagtc ttttccttac     8280

ccatggttgt ttatgttcgg atgtgatgtg agaactgtat cctagcaaga ttttaaaagg     8340

aagtatatga aagaagaacc tcagtggcaa atcctaacct tttatatttc tctacagggg     8400

cgcggcgtgg ggacaattca acgcgtctgt gaggggagcg tttccctgct cgcaggtctg     8460

cagcgaggag ccgtaatttt tgcttcgcgc cgtgcggcca tcaaaatgta tggatgcaaa     8520

tgattataca tggggatgta tgggctaaat gtacgggcga cagtcacatc atgcccctga     8580

gctgcgcacg tcaagactgt caaggagggt attctgggcc tccatgtcgc tggccgggtg     8640

acccggcggg gacgaggcaa gctaaacaga tctctagacc taataacttc gtatagcata     8700

cattatacga agttatatta agggttgtct taattaaggg tgcccaattc gccctatagt     8760

gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct     8820

ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc     8880

gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc     8940

gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc     9000

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc     9060

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt     9120

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg     9180

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt     9240

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta     9300

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt     9360

aacgcgaatt ttaacaaaat attaacgttt acaatttcct gatgcggtat tttctcctta     9420

cgcatctgtg cggtatttca caccgcatag atccgtcgag ttcaagagaa aaaaaaagaa     9480

aaagcaaaaa gaaaaaagga aagcgcgcct cgttcagaat gacacgtata gaatgatgca     9540

ttaccttgtc atcttcagta tcatactgtt cgtatacata cttactgaca ttcataggta     9600

tacatatata cacatgtata tatatcgtat gctgcagctt taaataatcg gtgtcaatgt     9660

ctgcccctat gtctgcccct aagaagatcg tcgttttgcc aggtgaccac gttggtcaag     9720

aaatcacagc cgaagccatt aaggttctta aagctatttc tgatgttcgt tccaatgtca     9780

agttcgattt cgaaaatcat ttaattggtg gtgctgctat cgatgctaca ggtgtcccac     9840

ttccagatga ggcgctggaa gcctccaaga aggttgatgc cgttttgtta ggtgctgtgg     9900

gtggtcctaa atggggtgcc ggtagtgtta gacctgaaca aggtttacta aaaatccgta     9960

aagaacttca attgtacgcc aacttaagac catgtaactt tgcatccgac tctcttttag    10020

acttatctcc aatcaagcca caatttgcta aaggtactga cttcgttgtt gtcagagaat    10080

tagtgggagg tatttacttt ggtaagagaa aggaagacga tggtgatggt gtcgcttggg    10140

atagtgaaca atacaccgtt ccagaagtgc aaagaatcac aagaatggcc gctttcatgg    10200

ccctacaaca tgagccacca ttgcctattt ggtccttgga taaagctaat gttttggcct    10260

cttcaagatt atggagaaaa actgtggagg aaaccatcaa gaacgaattc cctacattga    10320

aggttcaaca tcaattgatt gattctgccg ccatgatcct agttaagaac ccaacccacc    10380

taaatggtat tataatcacc agcaacatgt ttggtgatat catctccgat gaagcctccg    10440

ttatcccagg ttccttgggt ttgttgccat ctgcgtcctt ggcctctttg ccagacaaga    10500

acaccgcatt tggtttgtac gaaccatgcc acggttctgc tccagatttg ccaaagaata    10560

aggttgaccc tatcgccact atcttgtctg ctgcaatgat gttgaaattg tcattgaact    10620

tgcctgaaga aggtaaggcc attgaagatg cagttaaaaa ggttttggat gcaggtatca    10680

gaactggtga tttaggtggt tccaacagta ccaccgaagt cggtgatgct gtcgccgaag    10740

aagttaagaa aatccttgct taactttgcc ttcgtttatc ttgcctgctc attttttagt    10800

atattcttcg aagaaatcac attactttat ataatgtata attcattatg tgataatgcc    10860

aatcgctaag aaaaaaaaag agtcatccgc taggggaaaa aaaaaaatga aaatcattac    10920

cgaggcataa aaaaatatag agtgtactag aggaggccaa gagtaataga aaaagaaaat    10980

tgcgggaaag gactgtgtta tgacttccct gactaatgcc gtgttcaaac gatacctggc    11040

agtgactcct agcgctcacc aagctcttaa aacgggaatt tatggtgcac tctcagtaca    11100

atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacg cgctgacgcg    11160

ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg    11220

agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc    11280

gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta ggacggatcg    11340

cttgcctgta acttacacgc gcctcgtatc ttttaatgat ggaataattt gggaatttac    11400

tctgtgttta tttattttta tgttttgtat ttggatttta gaaagtaaat aaagaaggta    11460

gaagagttac ggaatgaaga aaaaaaaata aacaaaggtt taaaaaattt caacaaaaag    11520

cgtactttac atatatattt attagacaag aaaagcagat taaatagata tacattcgat    11580

taacgataag taaaatgtaa aatcacagga ttttcgtgtg tggtcttcta cacagacaag    11640

atgaaacaat tcggcattaa tacctgagag caggaagagc aagataaaag gtagtatttg    11700

ttggcgatcc ccctagagtc ttttacatct tcggaaaaca aaaactattt tttctttaat    11760

ttcttttttt actttctatt tttaatttat atatttatat taaaaaattt aaattataat    11820

tatttttata gcacgtgatg aaaaggaccc aggtggcact tttcggggaa atgtgcgcgg    11880

aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata    11940

accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg    12000

tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac    12060

gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact    12120

ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat    12180

gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga    12240

gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac    12300

agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat    12360

gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac    12420

cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct    12480

gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac    12540

gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga    12600

ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg    12660

gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact    12720

ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac    12780

tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta    12840

actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt    12900

taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga    12960

gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc    13020

tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt    13080

ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc    13140

gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc    13200

tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg    13260

cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg    13320

gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga    13380

actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc    13440

ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg    13500

gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg    13560

atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt    13620

tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc    13680

tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg    13740

aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc    13800

gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg    13860

gaaagcgggc agtgagcgca acgcaattaa tgtgagttac ctcactcatt aggcacccca    13920

ggctttacac tttatgcttc cggctcctat gttgtgtgga attgtgagcg gataacaatt    13980

tcacacagga aacagctatg accatgatta cgccaagcgc gcaat                    14025


<210>  3
<211>  684
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  Acc1 promoter
<222>  (1)..(463)

<220>
<221>  gRNA_3
<222>  (53)..(72)

<220>
<221>  gRNA_2
<222>  (265)..(284)

<220>
<221>  gRNA_1
<222>  (339)..(358)

<400>  3
ggtagaaact tgattttttc taattttctg cgctgtttcg ggaacggaaa aaaattaagc       60

tagaagacga atcggttatt atactattat atttgtatag tatagtagcg tgtcgtatcg      120

tatcgtgtcg tatcgtatcg tatcgttaaa agaaaataca cgaataaata ataatatgtg      180

gagaagaaaa agggaagttt cttgtctctt gctctgaatc tgaattccaa ttcaagttca      240

aattgttctc tagtttattg tccaaaaata aggatgaagc gggagggaag ggcagaggga      300

aaagttcgta tagtagaatg aataaacttt tataaacaca tgcaccgatc actcacagag      360

gataaaaaaa tggcacaaca aatatatata tatagatgca aatggcgatt gcaaattagg      420

gaattggctt tgttgttttt tatcttcagg taaactgtac gaaagggata aaaagagtag      480

aataaggaaa ggaaaattga agagagcaga acaattgtag aaccgataac aattgtgaca      540

gtgattgtgc taggctatac tgtgccagaa tacgactggg agtgctgttc ttcttatata      600

tcttggcgct gattgagcgt atagcctagt tcaccaagca gtagagagag tggcaatgag      660

cggttgaatt tcgactgcga cttg                                             684


<210>  4
<211>  971
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PGK1 promoter and integration sequences for Saccharomyces 
       cerevisiae Acc1 promoter


<220>
<221>  PGK1p
<222>  (7)..(750)

<400>  4
tgttttatat ttgttgtaaa aagtagataa ttacttcctt gatgatctgt aaaaaagaga       60

aaaagaaagc atctaagaac ttgaaaaact acgaattaga aaagaccaaa tatgtatttc      120

ttgcattgac caatttatgc aagtttatat atatgtaaat gtaagtttca cgaggttcta      180

ctaaactaaa ccaccccctt ggttagaaga aaagagtgtg tgagaacagg ctgttgttgt      240

cacacgattc ggacaattct gtttgaaaga gagagagtaa cagtacgatc gaacgaactt      300

tgctctggag atcacagtgg gcatcatagc atgtggtact aaaccctttc ccgccattcc      360

agaaccttcg attgcttgtt acaaaacctg tgagccgtcg ctaggacctt gttgtgtgac      420

gaaattggaa gctgcaatca ataggaagac aggaagtcga gcgtgtctgg gttttttcag      480

ttttgttctt tttgcaaaca aatcacgagc gacggtaatt tctttctcga taagaggcca      540

cgtgctttat gagggtaaca tcaattcaag aaggagggaa acacttcctt tttctggccc      600

tgataatagt atgagggtga agccaaaata aaggattcgc gcccaaatcg gcatctttaa      660

atgcaggtat gcgatagttc ctcactcttt ccttactcac gagtaattct tgcaaatgcc      720

tattatgcag atgttataat atctgtgcgt agggataaaa agagtagaat aaggaaagga      780

aaattgaaga gagcagaaca attgtagaac cgataacaat tgtgacagtg attgtgctag      840

gctatactgt gccagaatac gactgggagt gctgttcttc ttatatatct tggcgctgat      900

tgagcgtata gcctagttca ccaagcagta gagagagtgg caatgagcgg ttgaatttcg      960

actgcgactt g                                                           971


<210>  5
<211>  1724
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cassette with Saccharomyces cerevisiae Acc1 (S659A; S1167A) 
       coding sequence, regulatory sequences and integration sequences


<220>
<221>  T-G Ser659Ala
<222>  (108)..(108)

<220>
<221>  T-G ser1167ala
<222>  (1602)..(1602)

<400>  5
ggcgcgccga gggtaaaaga tacaagttca cggtcgctaa atccggtaat gaccgctaca       60

cattatttat caatggttct aaatgtgata tcatactgcg tcaactagct gatggtgggc      120

tgctgatcgc tatcggcgct aaatcgcata ccatctattg gaaagaagaa gttgctgcta      180

caagattatc cgttgactct atgactactt tgttggaagt tgaaaacgat ccaacccagt      240

tgcgtactcc atcccctggt aaattggtta aattcttggt ggaaaatggt gaacacatta      300

tcaagggcca accatatgca gaaattgaag ttatgaaaat gcaaatgcct ttggtttctc      360

aagaaaatgg tatcgtccag ttattaaagc aacctggttc taccattgtt gcaggtgata      420

tcatggctat tatgactctt gacgatccat ccaaggtcaa gcacgctcta ccatttgaag      480

gtatgctgcc agattttggt tctccagtta tcgaaggaac caaacctgcc tataaattca      540

agtcattagt gtctactttg gaaaacattt tgaagggtta tgacaaccaa gttattatga      600

acgcttcctt gcaacaattg atagaagttt tgagaaatcc aaaactgcct tactcagaat      660

ggaaactaca catctctgct ttacattcaa gattgcctgc taagctagat gaacaaatgg      720

aagagttagt tgcacgttct ttgagacgtg gtgctgtttt cccagctaga caattaagta      780

aattgattga tatggccgtg aagaatcctg aatacaaccc cgacaaattg ctgggcgcag      840

tcgtggaacc attggcggat attgctcata agtactctaa cgggttagaa gcccatgaac      900

attctatatt tgtccatttc ttggaagaat attacgaagt tgaaaagtta ttcaatggtc      960

caaatgttcg tgaggaaaat atcattctga aattgcgtga tgaaaaccct aaagatctag     1020

ataaagttgc gctaactgtt ttgtctcatt cgaaagtttc agcgaagaat aacctgatcc     1080

tagctatctt gaaacattat caaccattgt gcaagttatc ttctaaagtt tctgccattt     1140

tctctactcc tctacaacat attgttgaac tagaatctaa ggctaccgct aaggtcgctc     1200

tacaagcaag agaaattttg attcaaggcg ctttaccttc ggtcaaggaa agaactgaac     1260

aaattgaaca tatcttaaaa tcctctgttg tgaaggttgc ctatggctca tccaatccaa     1320

agcgctctga accagatttg aatatcttga aggacttgat cgattctaat tacgttgtgt     1380

tcgatgtttt acttcaattc ctaacccatc aagacccagt tgtgactgct gcagctgctc     1440

aagtctatat tcgtcgtgct tatcgtgctt acaccatagg agatattaga gttcacgaag     1500

gtgtcacagt tccaattgtt gaatggaaat tccaactacc ttcagctgcg ttctccacct     1560

ttccgactgt gaagtctaag atgggtatga acagggctgt tgctgtttca gatttgtcat     1620

atgttgcaaa cagtcagtca tctccgttaa gagaaggtat tttgatggct gtggatcatt     1680

tagatgatgt tgatgaaatt ttgtcacaaa gtttggggcg cgcc                      1724


<210>  6
<211>  3256
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cassette with Saccharomyces cerevisiae Maf1 coding sequence, 
       regulatory sequences and integration sequences


<220>
<221>  L0
<222>  (362)..(401)

<220>
<221>  Tef1
<222>  (402)..(895)

<220>
<221>  L1
<222>  (896)..(935)

<220>
<221>  MAF1
<222>  (936)..(2123)

<220>
<221>  L2
<222>  (2124)..(2163)

<220>
<221>  PRM9t
<222>  (2164)..(2701)

<220>
<221>  LTP2
<222>  (2702)..(2741)

<400>  6
aatgatttaa gcgtgcgtga agataacact acaatccatt ttaaagcaac atccacattg       60

agtgtataca ccacaaaggt tttttcaggg cgtttttctc gccactttat gttgaccaaa      120

attattaatg gaacttacaa cgtttccaaa agttagttaa atacatacgt ctatttacta      180

agcaagaaat atatcatgac aagcccaaat attatattgt tatgtttaca aaaaaaaaat      240

ggctatatac atcaagtctg gaggcttttt ataacaagca agtggggtaa cttagacata      300

agattgactt ctttgaattc aacaaaaata catacttttg atgatttcaa tggtagaagc      360

ataaacaaca aataatcata aaaattttag aactagacat aaagcaacag gcgcgttgga      420

cttttaattt tcgaggaccg cgaatcctta catcacaccc aatcccccac aagtgatccc      480

ccacacacca tagcttcaaa atgtttctac tcctttttta ctcttccaga ttttctcgga      540

ctccgcgcat cgccgtacca cttcaaaaca cccaagcaca gcatactaaa tttcccctct      600

ttcttcctct agggtgtcgt taattacccg tactaaaggt ttggaaaaga aaaaagagac      660

cgcctcgttt ctttttcttc gtcgaaaaag gcaataaaaa tttttatcac gtttcttttt      720

cttgaaaatt tttttttttg atttttttct ctttcgatga cctcccattg atatttaagt      780

taataaacgg tcttcaattt ctcaagtttc agtttcattt ttcttgttct attacaactt      840

tttttacttc ttgctcatta gaaagaaagc atagcaatct aatctaagtt ttaatctaga      900

aaatttatta taaaaggaag agaaataatt aaacaatgaa atttattgat gagctagata      960

tagagagagt gaatcaaact ctcaatttcg agacaaatga ctgtaaaatc gtgggcagtt     1020

gcgatatttt cacaacaaag gcggttgcat cagatagaaa attatataaa actattgatc     1080

agcatttgga tactatttta caggaaaatg agaattacaa tgctaccctt cagcaacagc     1140

tagctgctcc cgaaacaaac caatcaccct gctcgtcgcc attttattct aataggaggg     1200

atagcaactc tttttgggag caaaagagaa gaatatcttt tagtgaatac aatagcaata     1260

ataacactaa caacagtaat ggcaatagca gtaataacaa taactattct ggacctaatg     1320

gttcttctcc agcaactttt cccaaaagtg ccaagctaaa tgaccaaaat ttaaaagaat     1380

tagtctcgaa ttacgattct ggctctatga gctcatcgtc tcttgattct tcttctaaga     1440

atgatgagag gataagaaga aggagcagta gcagtattag cagtttcaaa agtggtaaat     1500

catcgaacaa taattacagt tctggtacag caaccaacaa tgttaacaaa agaagaaaat     1560

cttcgataaa cgaaaggcca agcaatttaa gtttgggtcc gtttggtccc ataaacgaac     1620

cgtcaagccg caaaatattt gcttatctga ttgctatcct caacgcttct tatcctgacc     1680

atgatttttc atcggttgag ccaacggatt ttgtcaaaac atcattgaaa acttttattt     1740

ccaaatttga aaacacctta tattctcttg gtagacaacc agaggaatgg gtctgggagg     1800

taattaattc tcacatgact ctttctgatt gcgtcctttt tcaatattca ccttcaaact     1860

cttttttgga agatgagcct ggctatcttt ggaatcttat aggttttctt tacaacagga     1920

aaaggaaaag agtggcttac ctttacttga tttgctcgcg tctaaattcg agtacaggcg     1980

aagtggaaga tgccttggca aaaaaacctc agggaaagct tataatagat gatggctcaa     2040

atgaatacga aggagaatac gatttcactt atgatgagaa tgtaatagat gataaatcag     2100

atcaagaaga atccctacag tagagacata aaactgaaac aacaccaatt aataatagac     2160

tttacagaag acgggagaca ctagcacaca actttaccag gcaaggtatt tgacgctagc     2220

atgtgtccaa ttcagtgtca tttatgattt tttgtagtag gatataaata tatacagcgc     2280

tccaaatagt gcggttgccc caaaaacacc acggaacctc atctgttctc gtactttgtt     2340

gtgacaaagt agctcactgc cttattatca cattttcatt atgcaacgct tcggaaaata     2400

cgatgttgaa aatgcctcta gagatgaaaa acaatcgtaa aagggtcctg cgtaattgaa     2460

acatttgatc agtatgcagt ggcacagaaa caaccaggaa tactatagtc ataggcaata     2520

caaggtatat attggctatg cagacccctc cagaaagtac cgacgtcaag ttagatacac     2580

ttaacgaacc tagtgcacat ttaattgaga aaaatgtggc tcttcctaag gacatattcc     2640

gttcgtactt gagttattgg atctatgaaa tcgctcgcta tacaccagtc atgattttgt     2700

ccttaaataa catactcatc actaaacatt cttaacaatc agaaaacaac gcgtcatgaa     2760

aaagagttac tgaaccttca gatcctactt attgtaatgc ttcgcgacat ccaatccatt     2820

taataatcaa tttaaaacta gagttggtag agttccttgt tgaacgtgat aacccaaaag     2880

cataatacga gtaatgtttc agtattgcta ttatatgttt acacaaggaa aacatataat     2940

aacaaacctc taatccggta gtacttaaga aactatagtt tctatgtaca aaaaggtaac     3000

tatgtaattc ttacatttac ataacgtata gaagggtcca ataaacttac taaacttact     3060

accttgttgt atataggcta gatcgtaatc cactacgtca acataaaaaa aacttaagaa     3120

gtttgaattt tatgtacaaa cagattgtta aaatataata taagattatg gaaacgaact     3180

tgctctaaaa aaaatttaaa gttttataaa atcctcgaac tatcgctgtt atacatgatg     3240

tccccaaagc gtgtac                                                     3256


<210>  7
<211>  4662
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cassette with Saccharomyces cerevisiae UPC2E888D coding sequence,
       regulatory sequences and integration sequences


<220>
<221>  L0
<222>  (401)..(440)

<220>
<221>  Tef1
<222>  (441)..(934)

<220>
<221>  L1
<222>  (935)..(974)

<220>
<221>  UPC2-1
<222>  (975)..(3701)

<220>
<221>  g-a G888D
<222>  (3637)..(3637)

<220>
<221>  L2
<222>  (3702)..(3741)

<220>
<221>  PRM9t
<222>  (3742)..(4279)

<220>
<221>  LTP2
<222>  (4280)..(4319)

<400>  7
cccagttgtt tgtagctggt tcatatttag cggcaattct ctgttgcgta aatgaaaata       60

ttaatgtaaa caaaaaaaga ccaaaacatt ttagcagtgt aagaaggtgt actgatacaa      120

aatgtgttta gagtctactg atatgttact gaccgttcgt tgggaaaaaa atactgtatc      180

atttattaat caaaagcgac ttttggtgga atattatgat atgtgttgtt aaaatatgac      240

gtaattttag aattgtctga ttcgtattca aatttggtga aggaataacg cagagttgac      300

aatttaatag aatggattaa tcgtaatttt cagaaacgta gaaaaagaaa aacaattaaa      360

acattatatt aagattattg atttgccttt taagggtcca taaacaacaa ataatcataa      420

aaattttaga actagacata aagcaacagg cgcgttggac ttttaatttt cgaggaccgc      480

gaatccttac atcacaccca atcccccaca agtgatcccc cacacaccat agcttcaaaa      540

tgtttctact ccttttttac tcttccagat tttctcggac tccgcgcatc gccgtaccac      600

ttcaaaacac ccaagcacag catactaaat ttcccctctt tcttcctcta gggtgtcgtt      660

aattacccgt actaaaggtt tggaaaagaa aaaagagacc gcctcgtttc tttttcttcg      720

tcgaaaaagg caataaaaat ttttatcacg tttctttttc ttgaaaattt ttttttttga      780

tttttttctc tttcgatgac ctcccattga tatttaagtt aataaacggt cttcaatttc      840

tcaagtttca gtttcatttt tcttgttcta ttacaacttt ttttacttct tgctcattag      900

aaagaaagca tagcaatcta atctaagttt taatctagaa aatttattat aaaaggaaga      960

gaaataatta aacaatgagc gaagtcggta tacagaatca caagaaagcg gtgacaaaac     1020

ccagaagaag agaaaaagtc atcgagctaa ttgaagtgga cggcaaaaag gtgagtacga     1080

cttcaaccgg taaacgtaaa ttccataaca aatcaaagaa tgggtgcgat aactgtaaaa     1140

gaagaagagt taagtgtgat gaagggaagc cagcctgtag gaagtgcaca aatatgaagt     1200

tggaatgtca gtatacacca atccatttaa ggaaaggtag aggagcaaca gtagtgaagt     1260

atgtcacgag aaaggcagac ggtagcgtgg agtctgattc atcggtagat ttacctccta     1320

cgatcaagaa ggagcagaca ccgttcaatg atatccaatc agcggtaaaa gcttcaggct     1380

catccaatga ttcctttcca tcaagcgcct ctacaactaa gagtgagagc gaggaaaagt     1440

catcggcccc tatagaggac aaaaacaata tgactcctct aagtatgggc ctccagggta     1500

ccatcaataa gaaagatatg atgaataact ttttctctca aaatggcact attggttttg     1560

gttctcctga aagattgaat tcaggtatcg atggcttact attaccgcca ttgccttctg     1620

gaaatatggg tgcgttccaa cttcagcaac agcagcaagt gcagcagcaa tctcaaccac     1680

agacccaagc gcagcaagca agtggaactc caaacgagag atatggttca ttcgatcttg     1740

cgggtagtcc tgcattgcaa tccacgggaa tgagcttatc aaatagtcta agcgggatgt     1800

tactatgtaa caggattcct tccggccaaa actacactca acaacaatta caatatcaat     1860

tacaccagca gctgcaattg caacagcatc agcaagttca gctgcagcag tatcaacaat     1920

tacgtcagga acaacaccaa caagttcagc aacaacaaca ggaacaactc cagcaatacc     1980

aacaacattt tttgcaacag cagcaacaag tactgcttca gcaagagcaa caacctaacg     2040

atgaggaagg tggcgttcag gaagaaaaca gcaaaaaggt aaaggaaggg cctttacaat     2100

cacaaacaag cgaaactact ttaaacagcg atgctgctac attacaagct gatgcattat     2160

ctcagttaag taagatgggg ctaagcctaa agtcgttaag tacctttcca acagctggta     2220

ttggtggtgt ttcctatgac tttcaggaac tgttaggtat taagtttcca ataaataacg     2280

gcaattcaag agctactaag gccagcaacg cagaggaagc tttggccaat atgcaagagc     2340

atcatgaacg tgcagctgct tctgtaaagg agaatgatgg tcagctctct gatacgaaga     2400

gtccagcgcc atcgaataac gcccaagggg gaagtgctag tattatggaa cctcaggcgg     2460

ctgatgcggt ttcgacaatg gcgcctatat caatgattga aagaaacatg aacagaaaca     2520

gcaacatttc tccatcaacg ccctctgcag tgttgaatga taggcaagag atgcaagatt     2580

ctataagttc tctaggaaat ctgacaaaag cagccttgga gaacaacgaa ccaacgataa     2640

gtttacaaac atcacagaca gagaatgaag acgatgcatc gcggcaagac atgacctcaa     2700

aaattaataa cgaagctgac cgaagttctg tttctgctgg taccagtaac atcgctaagc     2760

ttttagatct ttctaccaaa ggcaatctga acctgataga catgaaactg tttcatcatt     2820

attgcacaaa ggtctggcct acgattacag cggccaaagt ttctgggcct gaaatatgga     2880

gggactacat accggagtta gcatttgact atccattttt aatgcacgct ttgttggcat     2940

tcagtgccac ccatctttcg aggactgaaa ctggactgga gcaatacgtt tcatctcacc     3000

gcctagacgc tctgagatta ttaagagaag ctgttttaga aatatctgag aataacaccg     3060

atgcgctagt tgccagcgcc ctgatactaa tcatggactc gttagcaaat gctagtggta     3120

acggcactgt aggaaaccaa agtttgaata gcatgtcacc aagcgcttgg atctttcatg     3180

tcaaaggtgc tgcaacaatt ttaaccgctg tgtggccttt gagtgaaaga tctaaatttc     3240

ataacattat atctgttgat cttagcgatt taggcgatgt cattaaccct gatgttggaa     3300

caattactga attggtatgt tttgatgaaa gtattgccga tttgtatcct gtcggcttag     3360

attcgccata tttgataaca ctagcttatt tagataaatt gcaccgtgaa aaaaaccagg     3420

gtgattttat tctgcgggta tttacatttc cagcattgct agacaagaca ttcctggcat     3480

tactgatgac aggtgattta ggtgcaatga gaattatgag atcatattat aaactacttc     3540

gaggatttgc cacagaggtc aaggataaag tctggtttct cgaaggagtc acgcaggtgc     3600

tgcctcaaga cgttgatgag tacaggggag gtggtgatat gcatatgatg ctaggattac     3660

catcgatgac aacaacaaat ttctctgatt tttcgttatg aagacataaa actgaaacaa     3720

caccaattaa taatagactt tacagaagac gggagacact agcacacaac tttaccaggc     3780

aaggtatttg acgctagcat gtgtccaatt cagtgtcatt tatgattttt tgtagtagga     3840

tataaatata tacagcgctc caaatagtgc ggttgcccca aaaacaccac ggaacctcat     3900

ctgttctcgt actttgttgt gacaaagtag ctcactgcct tattatcaca ttttcattat     3960

gcaacgcttc ggaaaatacg atgttgaaaa tgcctctaga gatgaaaaac aatcgtaaaa     4020

gggtcctgcg taattgaaac atttgatcag tatgcagtgg cacagaaaca accaggaata     4080

ctatagtcat aggcaataca aggtatatat tggctatgca gacccctcca gaaagtaccg     4140

acgtcaagtt agatacactt aacgaaccta gtgcacattt aattgagaaa aatgtggctc     4200

ttcctaagga catattccgt tcgtacttga gttattggat ctatgaaatc gctcgctata     4260

caccagtcat gattttgtcc ttaaataaca tactcatcac taaacattct taacaatcac     4320

gatggatgat gattggttct tatcataatt tgatttcggc agaagcaata ttagaggtat     4380

tgttgtaacg aaattccaat gtcatctgct tagtattatt aatgttacct gcatattatc     4440

acatgccgct taaaaatgtg ttataagtat taaaatctag tgaaagttga aatgtaatct     4500

aataggataa tgaaacatat gaaacggaat gaggaataat cgttgtatta ctatgtagag     4560

atatcgattt cattttgagg attcctatat tcttggggag aacttctact atattctgta     4620

tacatgatat aatagccttt accaacaatg gaatgccaac aa                        4662


<210>  8
<211>  3564
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cassette with Aspergillus nidulans NpgA coding sequence, 
       regulatory sequences and integration sequences


<220>
<221>  LTP1 (L0)
<222>  (596)..(635)

<220>
<221>  Tef1p
<222>  (636)..(1129)

<220>
<221>  L1
<222>  (1130)..(1169)

<220>
<221>  NpgA
<222>  (1170)..(2201)

<220>
<221>  L2
<222>  (2205)..(2244)

<220>
<221>  PRM9t
<222>  (2245)..(2782)

<220>
<221>  LTP2
<222>  (2783)..(2822)

<400>  8
tcaatcaaag caacccacaa atcctaggct gaatcatgat atcgatggaa gcaatcaaca       60

attttatcaa gaccgcacca aagcacgact atctgacagg cggagttcat cattctggta      120

atgtagacgt gttacaatta agcggcaata aagaagatgg tagtttagta tggaaccata      180

cttttgttga tgtagacaac aatgtggtag ctaagtttga agacgctctc gaaaaacttg      240

aaagtttgca ccggcgctca tcctcatcca caggcaatga agaacacgct aacgtttaac      300

cgaggggagt cacttcataa tgatgtgaga aataagtgaa tattgtaata attgttggga      360

ctccattgtc aacaaaagct ataatgtagg tatacagtat atactagaag ttctcctcga      420

ggatcttgga atccacaaaa gggagtcgat aaatctatat aataaaaatt actttatctt      480

ctttcgtttt atacgttgtc gtttattatc ctattacgtt atcaatcttc gcatttcagc      540

tttcattaga tttgatgact gtttctcaaa ctttatgtca ttttcttaca ccgcataaac      600

aacaaataat cataaaaatt ttagaactag acataaagca acaggcgcgt tggactttta      660

attttcgagg accgcgaatc cttacatcac acccaatccc ccacaagtga tcccccacac      720

accatagctt caaaatgttt ctactccttt tttactcttc cagattttct cggactccgc      780

gcatcgccgt accacttcaa aacacccaag cacagcatac taaatttccc ctctttcttc      840

ctctagggtg tcgttaatta cccgtactaa aggtttggaa aagaaaaaag agaccgcctc      900

gtttcttttt cttcgtcgaa aaaggcaata aaaattttta tcacgtttct ttttcttgaa      960

aatttttttt tttgattttt ttctctttcg atgacctccc attgatattt aagttaataa     1020

acggtcttca atttctcaag tttcagtttc atttttcttg ttctattaca acttttttta     1080

cttcttgctc attagaaaga aagcatagca atctaatcta agttttaatc tagaaaattt     1140

attataaaag gaagagaaat aattaaacaa tggttcaaga tacctcttct gcttctacct     1200

ctccaatttt gactagatgg tacattgata ccagaccatt gactgcttct actgctgctt     1260

tgccattatt ggaaacttta caaccagccg atcaaatctc cgttcaaaag tactatcact     1320

tgaaggacaa gcacatgtct ttggcttcta acttgttgaa gtacttgttc gttcacagaa     1380

actgcagaat tccatggtcc tctatcgtta tttctagaac tccagatcca catagaaggc     1440

catgttatat tccaccatct ggttctcaag aggattcttt taaagatggt tacaccggta     1500

tcaacgtcga gtttaatgtt tctcatcaag cctccatggt tgctattgct ggtactgctt     1560

ttactccaaa ttctggtggt gattctaagt tgaaaccaga agttggtatc gatattacct     1620

gcgtcaacga aagacaaggt agaaatggtg aagaaaggtc cttggaatct ttgagacagt     1680

acatcgatat cttctccgaa gttttctcta ctgctgaaat ggccaacatt agaagattgg     1740

atggtgtctc ttcttcctca ttgtctgctg atagattggt tgattatggc tacaggttgt     1800

tctatactta ctgggctttg aaagaagcct acattaagat gactggtgaa gccttgttgg     1860

ctccatggtt gagagaattg gaattctcta atgttgttgc tccagctgct gttgctgaat     1920

ctggtgattc tgctggtgat tttggtgaac catatactgg tgttagaacc accttgtaca     1980

agaacttggt tgaagatgtt agaattgaag ttgctgcttt gggtggtgat tacttgtttg     2040

ctactgctgc tagaggtggt ggtattggtg cttcttctag accaggtggt ggtccagatg     2100

gttctggtat tagatctcaa gatccttgga ggccattcaa gaagttggat attgaaaggg     2160

atattcaacc atgtgctact ggtgtatgta actgcttgtc ttaaagacat aaaactgaaa     2220

caacaccaat taataataga ctttacagaa gacgggagac actagcacac aactttacca     2280

ggcaaggtat ttgacgctag catgtgtcca attcagtgtc atttatgatt ttttgtagta     2340

ggatataaat atatacagcg ctccaaatag tgcggttgcc ccaaaaacac cacggaacct     2400

catctgttct cgtactttgt tgtgacaaag tagctcactg ccttattatc acattttcat     2460

tatgcaacgc ttcggaaaat acgatgttga aaatgcctct agagatgaaa aacaatcgta     2520

aaagggtcct gcgtaattga aacatttgat cagtatgcag tggcacagaa acaaccagga     2580

atactatagt cataggcaat acaaggtata tattggctat gcagacccct ccagaaagta     2640

ccgacgtcaa gttagataca cttaacgaac ctagtgcaca tttaattgag aaaaatgtgg     2700

ctcttcctaa ggacatattc cgttcgtact tgagttattg gatctatgaa atcgctcgct     2760

atacaccagt catgattttg tccttaaata acatactcat cactaaacat tcttaacaat     2820

cagaaaatgc aaccgataaa acattataaa tcttcgcggt tatctggcat tgttattaac     2880

caaaaaaatg ccggcctatt acaagctact gttcaataaa tattgttgta atgaagacgg     2940

tccaactgta caaatacagc aaactgtcat atataaggtg tcttatgtga cagcacttgc     3000

gttattgtca gccggagtat gtctttgtcg cattctgggc tttttacttt ctgctcagaa     3060

ggaagtacga acaagaaaaa aaaatcacca atgcttccct tttcagtatt agtttcatat     3120

ttgtttacgt tcaaactcgt cgtttgcgcg ataacctcta aaaaagtcag ttacgtaact     3180

atatcaatca gagaatgcaa aaagcactat cataaaaatg tctctagggg atgtgagaca     3240

tgtcaattat aagaagtgat ggtgtcatag tatatatatc ataaatgatt atcaaagttt     3300

caatcctttg tattttctag tttagcgcca acttttgaca aaacctaaac tttagataat     3360

catcattctt acaattttta tctggatggc aataatctcc tatataaagc ccagataaac     3420

tgtaaaaaga atccatcact atttgaaaaa aagtcatctg gcacgtttaa ttatcagagc     3480

agaaatgatg aagggtgtta gcgccgtcca ttgatgcgcc tggtagtcat gatttacgta     3540

taactaacac atcatgagga cggc                                            3564


<210>  9
<211>  10584
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cassette with Dictyostelium discoideum DiPKS (G1516D; G1518A) 
       coding sequence,  regulatory sequences and integration sequences


<220>
<221>  LV3
<222>  (1)..(40)

<220>
<221>  S. cerevisiae GAL1 promoter
<222>  (41)..(482)

<220>
<221>  L1
<222>  (483)..(522)

<220>
<221>  DiPKS
<222>  (523)..(9966)

<220>
<221>  C-methyltransferase domain
<222>  (5050)..(5412)

<220>
<221>  Motif 1
<222>  (5050)..(5076)

<220>
<221>  G1516D
<222>  (5068)..(5070)

<220>
<221>  G1518A
<222>  (5074)..(5076)

<220>
<221>  Motif 2
<222>  (5309)..(5331)

<220>
<221>  Motif 3
<222>  (5389)..(5421)

<220>
<221>  L2
<222>  (9967)..(10006)

<220>
<221>  PRM9t
<222>  (10007)..(10544)

<220>
<221>  LV5
<222>  (10545)..(10584)

<400>  9
aggaatactc tgaataaaac aacttatata ataaaaatgc cggattagaa gccgccgagc       60

gggtgacagc cctccgaagg aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt      120

tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa caataaagat tctacaatac      180

tagcttttat ggttatgaag aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa      240

tgaacgaatc aaattaacaa ccataggatg ataatgcgat tagtttttta gccttatttc      300

tggggtaatt aatcagcgaa gcgatgattt ttgatctatt aacagatata taaatgcaaa      360

aactgcataa ccactttaac taatactttc aacattttcg gtttgtatta cttcttattc      420

aaatgtaata aaagtatcaa caaaaaattg ttaatatacc tctatacttt aacgtcaagg      480

agctagaaaa tttattataa aaggaagaga aataattaaa caatgaacaa gaactccaaa      540

atccagtccc caaactcttc tgatgttgct gttattggtg ttggttttag attcccaggt      600

aactctaatg acccagaatc tttgtggaac aacttgttgg atggtttcga tgctattacc      660

caagtcccaa aagaaagatg ggctacttct tttagagaga tgggtttgat caagaacaag      720

ttcggtggtt tcttgaagga ttctgaatgg aagaatttcg accctttgtt ctttggtatc      780

ggtccaaaag aagctccatt cattgatcca caacaaaggt tgttgttgtc catcgtttgg      840

gaatctttgg aagatgctta catcagacca gatgaattga gaggttctaa cactggtgtt      900

ttcatcggtg tttctaacaa cgattacacc aagttgggtt tccaagacaa ctactctatt      960

tctccataca ctatgaccgg ctctaactct tcattgaact ccaacagaat ttcctactgc     1020

ttcgatttta gaggtccatc cattactgtt gataccgctt gttcttcttc cttggtttct     1080

gttaatttgg gtgtccaatc catccaaatg ggtgaatgta agattgctat ttgcggtggt     1140

gttaacgctt tgtttgatcc atctacatct gttgcctttt ccaagttggg tgttttgtct     1200

gaaaatggca gatgcaactc ttttagtgat caagcctctg gttacgttag atctgaaggt     1260

gctggtgttg ttgttttgaa gtctttggaa caagctaagt tggatggtga tagaatctac     1320

ggtgttatca agggtgtttc ctctaatgaa gatggtgctt ctaatggtga caagaactct     1380

ttgactactc catcttgtga agcccaatcc attaacattt ctaaggctat ggaaaaggcc     1440

tccttgtctc catctgatat ctattacatt gaagcccatg gtactggtac tccagttggt     1500

gatccaattg aagttaaggc cttgtccaag atcttctcca actctaacaa caaccagttg     1560

aacaacttct ctaccgatgg taatgataac gatgatgatg atgacgataa cacctctcca     1620

gaaccattat tgattggctc attcaagtcc aacatcggtc atttggaatc tgctgctggt     1680

attgcttctt tgattaagtg ttgcttgatg ttgaagaaca ggatgttggt tccatccatt     1740

aactgctcta atttgaaccc atccattcca ttcgatcagt acaacatctc cgttatcaga     1800

gaaatcagac aattcccaac cgataagttg gttaacatcg gtatcaattc tttcggtttc     1860

ggtggttcta actgccattt gattattcaa gagtacaaca acaacttcaa gaacaactct     1920

accatctgca ataacaacaa caacaacaat aacaacatcg actacttgat cccaatctcc     1980

tctaagacta agaagtcctt ggataagtac ttgattttga tcaagaccaa ctccaactac     2040

cacaaggata tttctttcga tgacttcgtc aagttccaaa tcaagtctaa gcagtacaac     2100

ttgtccaaca gaatgactac cattgctaac gattggaact ccttcattaa gggttctaac     2160

gaattccaca acttgatcga atctaaggat ggtgaaggtg gttcttcatc ttctaacaga     2220

ggtattgatt ccgccaatca aatcaacact actactacct ctaccatcaa cgatatcgaa     2280

cctttgttgg ttttcgtttt ctgtggtcaa ggtccacaat ggaatggtat gattaagacc     2340

ttgtacaact ccgagaacgt tttcaagaac accgttgatc atgttgacag catcttgtac     2400

aagtacttcg gttactccat tttgaacgtc ttgtctaaga tcgatgataa cgacgattcc     2460

atcaaccatc caatagttgc tcaaccatct ttgttcttgt tgcaaattgg tttggtcgag     2520

ttgtttaagt actggggtat ctacccatct atctctgttg gtcattcttt cggtgaagtc     2580

tcttcttatt acttgtccgg tatcatctct ttggaaaccg cttgtaaaat cgtctacgtc     2640

agatcctcta atcagaacaa aactatgggt tccggtaaga tgttggttgt ttctatgggt     2700

tttaagcaat ggaacgatca attctctgct gaatggtccg atattgaaat tgcttgttac     2760

aacgctccag attccatagt tgttactggt aacgaagaaa gattgaaaga attgtccatc     2820

aagttgtccg acgaatccaa tcaaattttc aacaccttct tgaggtcccc atgttctttt     2880

cattcttccc atcaagaagt catcaagggt tctatgttcg aagagttgtc taacttgcaa     2940

tctactggtg aaaccgaaat ccctttgttc tctactgtta ctggtagaca agttttgtct     3000

ggtcatgtta ctgctcaaca catctacgat aatgttagag aaccagtctt gttccaaaag     3060

acgattgaat ccattacctc ctacatcaag tctcactacc catccaatca aaaggttatc     3120

tacgttgaaa ttgctccaca cccaaccttg ttttcattga tcaaaaagtc catcccatcc     3180

tccaacaaga attcctcttc tgttttgtgt ccattgaaca gaaaagaaaa ctccaacaac     3240

tcctacaaga agttcgtttc tcagttgtac ttcaacggtg ttaacgttga cttcaacttc     3300

cagttgaact ccatttgcga taacgttaac aacgatcacc atttgaacaa cgtcaagcaa     3360

aactccttca aagagactac caattccttg ccaagatacc aatgggaaca agatgaatat     3420

tggtccgaac cattgatctc cagaaagaat agattggaag gtccaactac ttccttgttg     3480

ggtcatagaa ttatctacag cttcccagtt ttccaatccg ttttggactt gcaatctgac     3540

aactacaaat acttgttgga ccacttggtt aacggtaagc cagtttttcc aggtgctggt     3600

tatttggata tcatcatcga attcttcgac taccaaaagc agcagttgaa ttcctctgat     3660

tcctctaact cctacatcat caacgttgac aagatccaat tcttgaaccc aattcacttg     3720

accgaaaaca agttgcaaac cttgcaatct tctttcgaac ctatcgttac taagaagtct     3780

gccttctctg ttaacttctt catcaaggat accgtcgagg atcaatctaa ggttaagtct     3840

atgtctgacg aaacttggac taacacttgt aaggctacca tttccttgga acaacaacag     3900

ccatctccat cttctacttt gactttgtct aagaagcaag acttgcagat cttgagaaac     3960

agatgcgata ttagcaagct agacaagttt gagttgtacg acaagatctc taagaatttg     4020

ggcttgcagt acaactcctt gtttcaagtt gttgatacca tcgaaactgg taaggattgc     4080

tcttttgcta ctttgtcttt gccagaagat actttgttca ccaccatttt gaacccatgc     4140

ttgttggata actgtttcca tggtttgttg accttgatca acgaaaaggg ttctttcgtt     4200

gtcgagtcca tttcttctgt ttctatctac ttggagaaca tcggttcctt caatcaaact     4260

tctgttggta acgtccagtt ctacttgtac accactattt ctaaagccac ctcctttagt     4320

tctgaaggta cttgtaagtt gttcaccaag gatggttcct tgattttgtc tatcggtaag     4380

ttcatcatca agtccaccaa tccaaagtct actaagacca acgaaactat cgaatctcca     4440

ttggacgaaa ccttctctat tgaatggcaa tctaaggatt ctccaattcc aaccccacaa     4500

caaatccaac aacaatctcc attgaactct aacccatcct tcattagatc taccatcttg     4560

aaggacatcc agttcgaaca atactgctcc tccattatcc acaaagaatt gatcaaccac     4620

gaaaagtaca agaaccagca atccttcgat atcaactcct tggaaaacca cttgaacgat     4680

gaccaattga tggaatcctt gtccatctcc aaagaatact tgagattctt caccaggatc     4740

atctccatca ttaagcaata cccaaagatc ttgaacgaaa aagagctaaa agaattgaaa     4800

gaaatcatcg aattgaagta cccatccgaa gttcagttgt tggaattcga agttatcgag     4860

aaggtgtcca tgattatccc aaagttgttg ttcgaaaacg acaagcaatc ttccatgacc     4920

ttgttccaag ataacttgtt gaccaggttc tactccaatt ctaactctac cagattctac     4980

ttggaaaggg tttccgaaat ggtcttggaa tctattagac caatcgtcag agaaaagagg     5040

gtgttcagaa ttttggaaat tggtgctgat acagcctctt tgtctaatgt tgttttgact     5100

aagttgaaca cctacttgtc caccttgaat tctaatggtg gttctggtta caacatcatc     5160

attgagtaca ccttcaccga tatttccgcc aacttcatta ttggtgaaat ccaagaaacc     5220

atgtgcaact tgtacccaaa cgttactttc aagttctccg tcttggactt ggagaaagag     5280

attattaact cctccgattt cttgatgggt gattacgata tagttttgat ggcctacgtt     5340

atccatgccg tttctaacat taagttctcc atcgaacagt tgtacaagtt gttgtctcca     5400

agaggttggt tgttgtgtat tgaacctaag tccaacgttg tgttctccga tttggttttc     5460

ggttgtttta atcagtggtg gaactactac gatgatatta gaactaccca ctgctccttg     5520

tctgaatctc aatggaatca gttgttgttg aaccagtcct tgaacaacga atcctcttct     5580

tcttctaact gttacggtgg tttctccaac gtttctttta ttggtggtga aaaggatgtc     5640

gactcccatt ctttcatatt gcactgccaa aaagaatcca tctcccaaat gaagttagcc     5700

accactatta acaacggttt gtcatctggt tccatcgtta tcgttttgaa ctctcaacaa     5760

ttgaccaaca tgaagtccta cccaaaggtt attgagtata ttcaagaggc tacctctttg     5820

tgcaagacca ttgaaattat cgattccaag gacgtcttga actctaccaa ttcagttttg     5880

gaaaagatcc aaaagtcctt gttggtgttc tgtttgttgg gttatgactt gttggagaac     5940

aactaccaag aacagtcttt cgaatacgtt aagttgttga acttgatctc tactaccgcc     6000

tcttcatcta atgataagaa accaccaaag gtcttgttga tcaccaagca atctgaaaga     6060

atctccaggt ctttctactc cagatccttg attggtattt ccagaacctc tatgaacgag     6120

tacccaaatt tgtccattac ctctatcgat ttggatacca acgactactc attgcagtct     6180

ttgttgaagc caatcttcag caactctaag ttttccgaca acgagttcat cttcaaaaag     6240

ggcttgatgt tcgtgtccag gatctttaag aacaagcagt tgctagaatc ctccaacgct     6300

tttgaaactg actcttctaa cttgtactgt aaggcctctt ctgacttgtc ttacaagtac     6360

gctattaagc agtctatgtt gaccgaaaat cagatcgaaa tcaaggttga atgcgtcggt     6420

attaacttca aggacaacct attctacaag ggcttgttgc cacaagaaat tttcagaatg     6480

ggtgacatct acaatccacc atatggtttg gaatgctctg gtgttattac cagaattggt     6540

tctaacgtca ccgaatactc agttggtcaa aatgtttttg gtttcgccag acattctttg     6600

ggttctcatg ttgttaccaa caaggatttg gttatcttga agccagatac catctcattt     6660

tctgaagctg cttctatccc agttgtttac tgtactgctt ggtactcctt gttcaacatt     6720

ggtcagttgt ctaacgaaga atccatccta attcattctg ctactggtgg tgtaggtttg     6780

gcttctttga atttgttgaa aatgaagaat cagcaacagc aaccattgac caatgtttat     6840

gctactgttg gctctaacga gaagaagaag ttcttgatcg ataacttcaa caacttgttc     6900

aaagaggacg gcgaaaacat tttctctacc agagacaaag aatactccaa ccagttggaa     6960

tccaagatcg atgttatttt gaacaccttg tccggtgaat tcgtcgaatc taatttcaag     7020

tccttgagat ccttcggtag attgattgat ttgtctgcta ctcacgttta cgccaatcaa     7080

caaattggtc taggtaactt caagttcgac cacttgtatt ctgctgttga cttggaaaga     7140

ttgatcgacg aaaaacctaa gttgttgcag tccatcttgc aaagaattac caactctatc     7200

gtcaacggtt ccttggaaaa aattccaatt accatcttcc catccaccga aactaaggat     7260

gctatcgaat tattgtccaa gagatcccat atcggtaaag ttgttgtaga ttgcaccgat     7320

atctctaagt gtaatcctgt tggtgatgtg atcaccaact tctctatgag attgccaaag     7380

ccaaactacc agttgaattt gaactccacc ttgttgatta ctggtcagtc tggtttgtct     7440

atccctttgt tgaattggtt gttgtctaag tctggtggta acgttaagaa cgttgtcatc     7500

atttctaagt ccaccatgaa gtggaagttg cagactatga tttcccattt cgtttccggt     7560

ttcggtatcc attttaacta cgttcaagtc gacatctcca actacgatgc tttgtctgaa     7620

gctattaagc aattgccatc tgatttgcca ccaatcacct ctgtttttca tttggctgct     7680

atctacaacg atgttccaat ggatcaagtt accatgtcta ccgttgaatc tgttcataac     7740

cctaaagttt tgggtgccgt taacttgcat agaatctctg tttcttttgg ttggaagttg     7800

aaccacttcg tcttgttctc ttctattact gctattaccg gttacccaga ccaatctatc     7860

tacaattctg ccaactctat tttggacgct ttgtccaact ttagaaggtt tatgggtttg     7920

ccatccttct ccattaactt gggtccaatg aaggatgaag gtaaggtttc taccaacaag     7980

agcatcaaga agctattcaa gtctagaggt ttgccaagcc tatccttgaa caagttattt     8040

ggtttgttgg aggtcgtcat caacaaccca tctaatcatg ttatcccatc ccaattgatt     8100

tgctccccaa tcgatttcaa gacctacatc gaatctttct caactatgag gccaaagttg     8160

ttacacttgc aacctaccat ttccaagcag caatcttcta tcattaacga ttctaccaag     8220

gcttcctcca acatttcatt gcaagataag atcacctcca aggtgtctga tttgttgtcc     8280

attccaatct ccaagatcaa cttcgatcat ccattgaaac actacggctt ggattctttg     8340

ttgaccgttc aattcaaatc ctggatcgac aaagaattcg aaaagaactt gttcacccat     8400

atccaattgg ccaccatctc tattaactca ttcttggaaa aggtgaacgg cttgtctaca     8460

aacaataaca acaacaacaa ttccaacgtc aagtcctctc catccattgt caaagaagaa     8520

atcgttacct tggacaagga tcaacaacca ttgctattga aagaacacca gcacattatc     8580

atctccccag atattagaat caacaagcca aagagggaat ccttgattag aaccccaatc     8640

ttgaacaaat tcaaccagat caccgaatcc attatcactc catctacacc atctttgtcc     8700

caatccgatg ttttgaaaac tccaccaatc aagtctttga acaacactaa gaactccagc     8760

ttgattaaca ccccaccaat tcaatctgtc caacaacatc aaaagcaaca acaaaaggtc     8820

caagtcatcc aacaacagca acaaccatta tccagattgt cctacaagag caacaacaac     8880

tctttcgttt tgggtatcgg tatttctgtt ccaggtgaac ctatttccca acaatccttg     8940

aaagactcca tctccaatga cttttctgat aaggctgaaa ctaacgagaa ggtcaagaga     9000

atctttgagc aatctcaaat caagaccaga cacttggtta gagattacac taagccagag     9060

aactccatca agttcagaca tttggaaacc attaccgatg tgaacaacca gttcaagaaa     9120

gttgttccag atttggctca acaagcctgt ttgagagctt tgaaagattg gggtggtgat     9180

aagggtgata ttacccatat agtttctgtt acctccaccg gtattatcat cccagatgtt     9240

aatttcaagt tgatcgactt gttgggcttg aacaaggatg ttgaaagagt gtctttgaac     9300

ctaatgggtt gtttggctgg tttgagttct ttgagaactg ctgcttcttt ggctaaggct     9360

tctccaagaa atagaatttt ggttgtctgt accgaagtct gctccttgca tttttctaat     9420

actgatggtg gtgatcaaat ggtcgcctct tctatttttg ctgatggttc tgctgcttac     9480

attattggtt gtaacccaag aattgaagaa accccattat acgaagtcat gtgctccatt     9540

aacagatctt tcccaaatac cgaaaacgcc atggtttggg atttggaaaa agaaggttgg     9600

aacttgggtt tggatgcttc tattccaatt gtcattggtt ctggtattga agccttcgtt     9660

gatactttgt tggataaggc taagttgcaa acttccactg ctatttctgc taaggattgc     9720

gaattcttga ttcatactgg tggcaagtcc atcttgatga acatcgaaaa ttccttgggt     9780

atcgacccaa agcaaactaa gaatacttgg gatgtttacc atgcctacgg caatatgtca     9840

tctgcctctg ttattttcgt tatggatcat gccagaaagt ccaagtcttt gccaacttac     9900

tcaatttctt tggcttttgg tccaggtttg gcttttgaag gttgtttctt gaagaacgtc     9960

gtctaaagac ataaaactga aacaacacca attaataata gactttacag aagacgggag    10020

acactagcac acaactttac caggcaaggt atttgacgct agcatgtgtc caattcagtg    10080

tcatttatga ttttttgtag taggatataa atatatacag cgctccaaat agtgcggttg    10140

ccccaaaaac accacggaac ctcatctgtt ctcgtacttt gttgtgacaa agtagctcac    10200

tgccttatta tcacattttc attatgcaac gcttcggaaa atacgatgtt gaaaatgcct    10260

ctagagatga aaaacaatcg taaaagggtc ctgcgtaatt gaaacatttg atcagtatgc    10320

agtggcacag aaacaaccag gaatactata gtcataggca atacaaggta tatattggct    10380

atgcagaccc ctccagaaag taccgacgtc aagttagata cacttaacga acctagtgca    10440

catttaattg agaaaaatgt ggctcttcct aaggacatat tccgttcgta cttgagttat    10500

tggatctatg aaatcgctcg ctatacacca gtcatgattt tgtccctctt tatattacat    10560

caaaataaga aaataattat aaca                                           10584


<210>  10
<211>  10584
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cassette with Dictyostelium discoideum DiPKS (G1516R) coding 
       sequence,  regulatory sequences and integration sequences


<220>
<221>  LV3
<222>  (1)..(40)

<220>
<221>  S. cerevisiae GAL1 promoter
<222>  (41)..(482)

<220>
<221>  L1
<222>  (483)..(522)

<220>
<221>  DiPKS
<222>  (523)..(9966)

<220>
<221>  C-methyltransferase domain
<222>  (5050)..(5412)

<220>
<221>  G1516R
<222>  (5069)..(5070)

<220>
<221>  Motif 2
<222>  (5309)..(5331)

<220>
<221>  Motif 3
<222>  (5389)..(5421)

<220>
<221>  Type III PKS domain
<222>  (8881)..(9966)

<220>
<221>  L2
<222>  (9967)..(10006)

<220>
<221>  PRM9t
<222>  (10007)..(10544)

<220>
<221>  LV5
<222>  (10545)..(10584)

<400>  10
aggaatactc tgaataaaac aacttatata ataaaaatgc cggattagaa gccgccgagc       60

gggtgacagc cctccgaagg aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt      120

tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa caataaagat tctacaatac      180

tagcttttat ggttatgaag aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa      240

tgaacgaatc aaattaacaa ccataggatg ataatgcgat tagtttttta gccttatttc      300

tggggtaatt aatcagcgaa gcgatgattt ttgatctatt aacagatata taaatgcaaa      360

aactgcataa ccactttaac taatactttc aacattttcg gtttgtatta cttcttattc      420

aaatgtaata aaagtatcaa caaaaaattg ttaatatacc tctatacttt aacgtcaagg      480

agctagaaaa tttattataa aaggaagaga aataattaaa caatgaacaa gaactccaaa      540

atccagtccc caaactcttc tgatgttgct gttattggtg ttggttttag attcccaggt      600

aactctaatg acccagaatc tttgtggaac aacttgttgg atggtttcga tgctattacc      660

caagtcccaa aagaaagatg ggctacttct tttagagaga tgggtttgat caagaacaag      720

ttcggtggtt tcttgaagga ttctgaatgg aagaatttcg accctttgtt ctttggtatc      780

ggtccaaaag aagctccatt cattgatcca caacaaaggt tgttgttgtc catcgtttgg      840

gaatctttgg aagatgctta catcagacca gatgaattga gaggttctaa cactggtgtt      900

ttcatcggtg tttctaacaa cgattacacc aagttgggtt tccaagacaa ctactctatt      960

tctccataca ctatgaccgg ctctaactct tcattgaact ccaacagaat ttcctactgc     1020

ttcgatttta gaggtccatc cattactgtt gataccgctt gttcttcttc cttggtttct     1080

gttaatttgg gtgtccaatc catccaaatg ggtgaatgta agattgctat ttgcggtggt     1140

gttaacgctt tgtttgatcc atctacatct gttgcctttt ccaagttggg tgttttgtct     1200

gaaaatggca gatgcaactc ttttagtgat caagcctctg gttacgttag atctgaaggt     1260

gctggtgttg ttgttttgaa gtctttggaa caagctaagt tggatggtga tagaatctac     1320

ggtgttatca agggtgtttc ctctaatgaa gatggtgctt ctaatggtga caagaactct     1380

ttgactactc catcttgtga agcccaatcc attaacattt ctaaggctat ggaaaaggcc     1440

tccttgtctc catctgatat ctattacatt gaagcccatg gtactggtac tccagttggt     1500

gatccaattg aagttaaggc cttgtccaag atcttctcca actctaacaa caaccagttg     1560

aacaacttct ctaccgatgg taatgataac gatgatgatg atgacgataa cacctctcca     1620

gaaccattat tgattggctc attcaagtcc aacatcggtc atttggaatc tgctgctggt     1680

attgcttctt tgattaagtg ttgcttgatg ttgaagaaca ggatgttggt tccatccatt     1740

aactgctcta atttgaaccc atccattcca ttcgatcagt acaacatctc cgttatcaga     1800

gaaatcagac aattcccaac cgataagttg gttaacatcg gtatcaattc tttcggtttc     1860

ggtggttcta actgccattt gattattcaa gagtacaaca acaacttcaa gaacaactct     1920

accatctgca ataacaacaa caacaacaat aacaacatcg actacttgat cccaatctcc     1980

tctaagacta agaagtcctt ggataagtac ttgattttga tcaagaccaa ctccaactac     2040

cacaaggata tttctttcga tgacttcgtc aagttccaaa tcaagtctaa gcagtacaac     2100

ttgtccaaca gaatgactac cattgctaac gattggaact ccttcattaa gggttctaac     2160

gaattccaca acttgatcga atctaaggat ggtgaaggtg gttcttcatc ttctaacaga     2220

ggtattgatt ccgccaatca aatcaacact actactacct ctaccatcaa cgatatcgaa     2280

cctttgttgg ttttcgtttt ctgtggtcaa ggtccacaat ggaatggtat gattaagacc     2340

ttgtacaact ccgagaacgt tttcaagaac accgttgatc atgttgacag catcttgtac     2400

aagtacttcg gttactccat tttgaacgtc ttgtctaaga tcgatgataa cgacgattcc     2460

atcaaccatc caatagttgc tcaaccatct ttgttcttgt tgcaaattgg tttggtcgag     2520

ttgtttaagt actggggtat ctacccatct atctctgttg gtcattcttt cggtgaagtc     2580

tcttcttatt acttgtccgg tatcatctct ttggaaaccg cttgtaaaat cgtctacgtc     2640

agatcctcta atcagaacaa aactatgggt tccggtaaga tgttggttgt ttctatgggt     2700

tttaagcaat ggaacgatca attctctgct gaatggtccg atattgaaat tgcttgttac     2760

aacgctccag attccatagt tgttactggt aacgaagaaa gattgaaaga attgtccatc     2820

aagttgtccg acgaatccaa tcaaattttc aacaccttct tgaggtcccc atgttctttt     2880

cattcttccc atcaagaagt catcaagggt tctatgttcg aagagttgtc taacttgcaa     2940

tctactggtg aaaccgaaat ccctttgttc tctactgtta ctggtagaca agttttgtct     3000

ggtcatgtta ctgctcaaca catctacgat aatgttagag aaccagtctt gttccaaaag     3060

acgattgaat ccattacctc ctacatcaag tctcactacc catccaatca aaaggttatc     3120

tacgttgaaa ttgctccaca cccaaccttg ttttcattga tcaaaaagtc catcccatcc     3180

tccaacaaga attcctcttc tgttttgtgt ccattgaaca gaaaagaaaa ctccaacaac     3240

tcctacaaga agttcgtttc tcagttgtac ttcaacggtg ttaacgttga cttcaacttc     3300

cagttgaact ccatttgcga taacgttaac aacgatcacc atttgaacaa cgtcaagcaa     3360

aactccttca aagagactac caattccttg ccaagatacc aatgggaaca agatgaatat     3420

tggtccgaac cattgatctc cagaaagaat agattggaag gtccaactac ttccttgttg     3480

ggtcatagaa ttatctacag cttcccagtt ttccaatccg ttttggactt gcaatctgac     3540

aactacaaat acttgttgga ccacttggtt aacggtaagc cagtttttcc aggtgctggt     3600

tatttggata tcatcatcga attcttcgac taccaaaagc agcagttgaa ttcctctgat     3660

tcctctaact cctacatcat caacgttgac aagatccaat tcttgaaccc aattcacttg     3720

accgaaaaca agttgcaaac cttgcaatct tctttcgaac ctatcgttac taagaagtct     3780

gccttctctg ttaacttctt catcaaggat accgtcgagg atcaatctaa ggttaagtct     3840

atgtctgacg aaacttggac taacacttgt aaggctacca tttccttgga acaacaacag     3900

ccatctccat cttctacttt gactttgtct aagaagcaag acttgcagat cttgagaaac     3960

agatgcgata ttagcaagct agacaagttt gagttgtacg acaagatctc taagaatttg     4020

ggcttgcagt acaactcctt gtttcaagtt gttgatacca tcgaaactgg taaggattgc     4080

tcttttgcta ctttgtcttt gccagaagat actttgttca ccaccatttt gaacccatgc     4140

ttgttggata actgtttcca tggtttgttg accttgatca acgaaaaggg ttctttcgtt     4200

gtcgagtcca tttcttctgt ttctatctac ttggagaaca tcggttcctt caatcaaact     4260

tctgttggta acgtccagtt ctacttgtac accactattt ctaaagccac ctcctttagt     4320

tctgaaggta cttgtaagtt gttcaccaag gatggttcct tgattttgtc tatcggtaag     4380

ttcatcatca agtccaccaa tccaaagtct actaagacca acgaaactat cgaatctcca     4440

ttggacgaaa ccttctctat tgaatggcaa tctaaggatt ctccaattcc aaccccacaa     4500

caaatccaac aacaatctcc attgaactct aacccatcct tcattagatc taccatcttg     4560

aaggacatcc agttcgaaca atactgctcc tccattatcc acaaagaatt gatcaaccac     4620

gaaaagtaca agaaccagca atccttcgat atcaactcct tggaaaacca cttgaacgat     4680

gaccaattga tggaatcctt gtccatctcc aaagaatact tgagattctt caccaggatc     4740

atctccatca ttaagcaata cccaaagatc ttgaacgaaa aagagctaaa agaattgaaa     4800

gaaatcatcg aattgaagta cccatccgaa gttcagttgt tggaattcga agttatcgag     4860

aaggtgtcca tgattatccc aaagttgttg ttcgaaaacg acaagcaatc ttccatgacc     4920

ttgttccaag ataacttgtt gaccaggttc tactccaatt ctaactctac cagattctac     4980

ttggaaaggg tttccgaaat ggtcttggaa tctattagac caatcgtcag agaaaagagg     5040

gtgttcagaa ttttagagat cggtgctcgt acaggctctt tgtctaatgt tgttttgact     5100

aagttgaaca cctacttgtc caccttgaat tctaatggtg gttctggtta caacatcatc     5160

attgagtaca ccttcaccga tatttccgcc aacttcatta ttggtgaaat ccaagaaacc     5220

atgtgcaact tgtacccaaa cgttactttc aagttctccg tcttggactt ggagaaagag     5280

attattaact cctccgattt cttgatgggt gattacgata tagttttgat ggcctacgtt     5340

atccatgccg tttctaacat taagttctcc atcgaacagt tgtacaagtt gttgtctcca     5400

agaggttggt tgttgtgtat tgaacctaag tccaacgttg tgttctccga tttggttttc     5460

ggttgtttta atcagtggtg gaactactac gatgatatta gaactaccca ctgctccttg     5520

tctgaatctc aatggaatca gttgttgttg aaccagtcct tgaacaacga atcctcttct     5580

tcttctaact gttacggtgg tttctccaac gtttctttta ttggtggtga aaaggatgtc     5640

gactcccatt ctttcatatt gcactgccaa aaagaatcca tctcccaaat gaagttagcc     5700

accactatta acaacggttt gtcatctggt tccatcgtta tcgttttgaa ctctcaacaa     5760

ttgaccaaca tgaagtccta cccaaaggtt attgagtata ttcaagaggc tacctctttg     5820

tgcaagacca ttgaaattat cgattccaag gacgtcttga actctaccaa ttcagttttg     5880

gaaaagatcc aaaagtcctt gttggtgttc tgtttgttgg gttatgactt gttggagaac     5940

aactaccaag aacagtcttt cgaatacgtt aagttgttga acttgatctc tactaccgcc     6000

tcttcatcta atgataagaa accaccaaag gtcttgttga tcaccaagca atctgaaaga     6060

atctccaggt ctttctactc cagatccttg attggtattt ccagaacctc tatgaacgag     6120

tacccaaatt tgtccattac ctctatcgat ttggatacca acgactactc attgcagtct     6180

ttgttgaagc caatcttcag caactctaag ttttccgaca acgagttcat cttcaaaaag     6240

ggcttgatgt tcgtgtccag gatctttaag aacaagcagt tgctagaatc ctccaacgct     6300

tttgaaactg actcttctaa cttgtactgt aaggcctctt ctgacttgtc ttacaagtac     6360

gctattaagc agtctatgtt gaccgaaaat cagatcgaaa tcaaggttga atgcgtcggt     6420

attaacttca aggacaacct attctacaag ggcttgttgc cacaagaaat tttcagaatg     6480

ggtgacatct acaatccacc atatggtttg gaatgctctg gtgttattac cagaattggt     6540

tctaacgtca ccgaatactc agttggtcaa aatgtttttg gtttcgccag acattctttg     6600

ggttctcatg ttgttaccaa caaggatttg gttatcttga agccagatac catctcattt     6660

tctgaagctg cttctatccc agttgtttac tgtactgctt ggtactcctt gttcaacatt     6720

ggtcagttgt ctaacgaaga atccatccta attcattctg ctactggtgg tgtaggtttg     6780

gcttctttga atttgttgaa aatgaagaat cagcaacagc aaccattgac caatgtttat     6840

gctactgttg gctctaacga gaagaagaag ttcttgatcg ataacttcaa caacttgttc     6900

aaagaggacg gcgaaaacat tttctctacc agagacaaag aatactccaa ccagttggaa     6960

tccaagatcg atgttatttt gaacaccttg tccggtgaat tcgtcgaatc taatttcaag     7020

tccttgagat ccttcggtag attgattgat ttgtctgcta ctcacgttta cgccaatcaa     7080

caaattggtc taggtaactt caagttcgac cacttgtatt ctgctgttga cttggaaaga     7140

ttgatcgacg aaaaacctaa gttgttgcag tccatcttgc aaagaattac caactctatc     7200

gtcaacggtt ccttggaaaa aattccaatt accatcttcc catccaccga aactaaggat     7260

gctatcgaat tattgtccaa gagatcccat atcggtaaag ttgttgtaga ttgcaccgat     7320

atctctaagt gtaatcctgt tggtgatgtg atcaccaact tctctatgag attgccaaag     7380

ccaaactacc agttgaattt gaactccacc ttgttgatta ctggtcagtc tggtttgtct     7440

atccctttgt tgaattggtt gttgtctaag tctggtggta acgttaagaa cgttgtcatc     7500

atttctaagt ccaccatgaa gtggaagttg cagactatga tttcccattt cgtttccggt     7560

ttcggtatcc attttaacta cgttcaagtc gacatctcca actacgatgc tttgtctgaa     7620

gctattaagc aattgccatc tgatttgcca ccaatcacct ctgtttttca tttggctgct     7680

atctacaacg atgttccaat ggatcaagtt accatgtcta ccgttgaatc tgttcataac     7740

cctaaagttt tgggtgccgt taacttgcat agaatctctg tttcttttgg ttggaagttg     7800

aaccacttcg tcttgttctc ttctattact gctattaccg gttacccaga ccaatctatc     7860

tacaattctg ccaactctat tttggacgct ttgtccaact ttagaaggtt tatgggtttg     7920

ccatccttct ccattaactt gggtccaatg aaggatgaag gtaaggtttc taccaacaag     7980

agcatcaaga agctattcaa gtctagaggt ttgccaagcc tatccttgaa caagttattt     8040

ggtttgttgg aggtcgtcat caacaaccca tctaatcatg ttatcccatc ccaattgatt     8100

tgctccccaa tcgatttcaa gacctacatc gaatctttct caactatgag gccaaagttg     8160

ttacacttgc aacctaccat ttccaagcag caatcttcta tcattaacga ttctaccaag     8220

gcttcctcca acatttcatt gcaagataag atcacctcca aggtgtctga tttgttgtcc     8280

attccaatct ccaagatcaa cttcgatcat ccattgaaac actacggctt ggattctttg     8340

ttgaccgttc aattcaaatc ctggatcgac aaagaattcg aaaagaactt gttcacccat     8400

atccaattgg ccaccatctc tattaactca ttcttggaaa aggtgaacgg cttgtctaca     8460

aacaataaca acaacaacaa ttccaacgtc aagtcctctc catccattgt caaagaagaa     8520

atcgttacct tggacaagga tcaacaacca ttgctattga aagaacacca gcacattatc     8580

atctccccag atattagaat caacaagcca aagagggaat ccttgattag aaccccaatc     8640

ttgaacaaat tcaaccagat caccgaatcc attatcactc catctacacc atctttgtcc     8700

caatccgatg ttttgaaaac tccaccaatc aagtctttga acaacactaa gaactccagc     8760

ttgattaaca ccccaccaat tcaatctgtc caacaacatc aaaagcaaca acaaaaggtc     8820

caagtcatcc aacaacagca acaaccatta tccagattgt cctacaagag caacaacaac     8880

tctttcgttt tgggtatcgg tatttctgtt ccaggtgaac ctatttccca acaatccttg     8940

aaagactcca tctccaatga cttttctgat aaggctgaaa ctaacgagaa ggtcaagaga     9000

atctttgagc aatctcaaat caagaccaga cacttggtta gagattacac taagccagag     9060

aactccatca agttcagaca tttggaaacc attaccgatg tgaacaacca gttcaagaaa     9120

gttgttccag atttggctca acaagcctgt ttgagagctt tgaaagattg gggtggtgat     9180

aagggtgata ttacccatat agtttctgtt acctccaccg gtattatcat cccagatgtt     9240

aatttcaagt tgatcgactt gttgggcttg aacaaggatg ttgaaagagt gtctttgaac     9300

ctaatgggtt gtttggctgg tttgagttct ttgagaactg ctgcttcttt ggctaaggct     9360

tctccaagaa atagaatttt ggttgtctgt accgaagtct gctccttgca tttttctaat     9420

actgatggtg gtgatcaaat ggtcgcctct tctatttttg ctgatggttc tgctgcttac     9480

attattggtt gtaacccaag aattgaagaa accccattat acgaagtcat gtgctccatt     9540

aacagatctt tcccaaatac cgaaaacgcc atggtttggg atttggaaaa agaaggttgg     9600

aacttgggtt tggatgcttc tattccaatt gtcattggtt ctggtattga agccttcgtt     9660

gatactttgt tggataaggc taagttgcaa acttccactg ctatttctgc taaggattgc     9720

gaattcttga ttcatactgg tggcaagtcc atcttgatga acatcgaaaa ttccttgggt     9780

atcgacccaa agcaaactaa gaatacttgg gatgtttacc atgcctacgg caatatgtca     9840

tctgcctctg ttattttcgt tatggatcat gccagaaagt ccaagtcttt gccaacttac     9900

tcaatttctt tggcttttgg tccaggtttg gcttttgaag gttgtttctt gaagaacgtc     9960

gtctaaagac ataaaactga aacaacacca attaataata gactttacag aagacgggag    10020

acactagcac acaactttac caggcaaggt atttgacgct agcatgtgtc caattcagtg    10080

tcatttatga ttttttgtag taggatataa atatatacag cgctccaaat agtgcggttg    10140

ccccaaaaac accacggaac ctcatctgtt ctcgtacttt gttgtgacaa agtagctcac    10200

tgccttatta tcacattttc attatgcaac gcttcggaaa atacgatgtt gaaaatgcct    10260

ctagagatga aaaacaatcg taaaagggtc ctgcgtaatt gaaacatttg atcagtatgc    10320

agtggcacag aaacaaccag gaatactata gtcataggca atacaaggta tatattggct    10380

atgcagaccc ctccagaaag taccgacgtc aagttagata cacttaacga acctagtgca    10440

catttaattg agaaaaatgt ggctcttcct aaggacatat tccgttcgta cttgagttat    10500

tggatctatg aaatcgctcg ctatacacca gtcatgattt tgtccctctt tatattacat    10560

caaaataaga aaataattat aaca                                           10584


<210>  11
<211>  6034
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid


<220>
<221>  LV5
<222>  (1)..(40)

<220>
<221>  pYES2-LEU2
<222>  (1915)..(4123)

<220>
<221>  LEU2 ORF
<222>  (1996)..(3090)

<220>
<221>  LEU2 promoter
<222>  (3091)..(3999)

<220>
<221>  misc_feature
<222>  (3759)..(3760)
<223>  n is a, c, g, or t

<220>
<221>  LV3
<222>  (5995)..(6034)

<400>  11
cctctttata ttacatcaaa ataagaaaat aattataaca cctgcattaa tgaatcggcc       60

aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact      120

cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac      180

ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa      240

agcccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg      300

acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa      360

gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc      420

ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac      480

gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac      540

cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg      600

taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt      660

atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga      720

cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct      780

cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga      840

ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg      900

ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct      960

tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt     1020

aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc     1080

tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagc     1140

gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag     1200

atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt     1260

tatccgcctc cattcagtct attaattgtt gccgggaagc tagagtaagt agttcgccag     1320

ttaatagttt gcgcaacgtt gttggcattg ctacaggcat cgtggtgtca ctctcgtcgt     1380

ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca     1440

tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg     1500

ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat     1560

ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta     1620

tgcggcgacc gagttgctct tgcccggcgt caatacggga taatagtgta tcacatagca     1680

gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct     1740

taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat     1800

cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa     1860

agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatgggtaa     1920

taactgatat aattaaattg aagctctaat ttgtgagttt agtatacatg catttactta     1980

taatacagtt ttttattaag caaggatttt cttaacttct tcggcgacag catcaccgac     2040

ttcggtggta ctgttggaac cacctaaatc accagttctg atacctgcat ccaaaacctt     2100

tttaactgca tcttcaatgg ccttaccttc ttcaggcaag ttcaatgaca atttcaacat     2160

cattgcagca gacaagatag tggcgatagg gttgacctta ttctttggca aatctggagc     2220

agaaccgtgg catggttcgt acaaaccaaa tgcggtgttc ttgtctggca aagaggccaa     2280

ggacgcagat ggcaacaaac ccaaggaacc tgggataacg gaggcttcat cggagatgat     2340

atcaccaaac atgttgctgg tgattataat accatttagg tgggttgggt tcttaactag     2400

gatcatggcg gcagaatcaa tcaattgatg ttgaaccttc aatgtaggga attcgttctt     2460

gatggtttcc tccacagttt ttctccataa tcttgaagag gccaaaacat tagctttatc     2520

caaggaccaa ataggcaatg gtggctcatg ttgtagggcc atgaaagcgg ccattcttgt     2580

gattctttgc acttctggaa cggtgtattg ttcactatcc caagcgacac catcaccatc     2640

gtcttccttt ctcttaccaa agtaaatacc tcccactaat tctctgacaa caacgaagtc     2700

agtaccttta gcaaattgtg gcttgattgg agataagtct aaaagagagt cggatgcaaa     2760

gttacatggt cttaagttgg cgtacaattg aagttcttta cggattttta gtaaaccttg     2820

ttcaggtcta acactaccgg taccccattt aggaccaccc acagcaccta acaaaacggc     2880

atcagccttc ttggaggctt ccagcgcctc atctggaagt ggaacacctg tagcatcgat     2940

agcagcacca ccaattaaat gattttcgaa atcgaacttg acattggaac gaacatcaga     3000

aatagcttta agaaccttaa tggcttcggc tgtgatttct tgaccaacgt ggtcacctgg     3060

caaaacgacg atcttcttag gggcagacat tagaatggta tatccttgaa atatatatat     3120

atattgctga aatgtaaaag gtaagaaaag ttagaaagta agacgattgc taaccaccta     3180

ttggaaaaaa caataggtcc ttaaataata ttgtcaactt caagtattgt gatgcaagca     3240

tttagtcatg aacgcttctc tattctatat gaaaagccgg ttccggcgct ctcacctttc     3300

ctttttctcc caatttttca gttgaaaaag gtatatgcgt caggcgacct ctgaaattaa     3360

caaaaaattt ccagtcatcg aatttgattc tgtgcgatag cgcccctgtg tgttctcgtt     3420

atgttgagga aaaaaataat ggttgctaag agattcgaac tcttgcatct tacgatacct     3480

gagtattccc acagttaact gcggtcaaga tatttcttga atcaggcgcc ttagaccgct     3540

cggccaaaca accaattact tgttgagaaa tagagtataa ttatcctata aatataacgt     3600

ttttgaacac acatgaacaa ggaagtacag gacaattgat tttgaagaga atgtggattt     3660

tgatgtaatt gttgggattc catttttaat aaggcaataa tattaggtat gtagatatac     3720

tagaagttct cctcgaggat ttaggaatcc ataaaaggnn atctgcaatt ctacacaatt     3780

ctagaaatat tattatcatc attttatatg ttaatattca ttgatcctat tacattatca     3840

atccttgcgt ttcagcttcc actaatttag atgactattt ctcatcattt gcgtcatctt     3900

ctaacaccgt atatgataat atactagtaa cgtaaatact agttagtaga tgatagttga     3960

tttttattcc aacataccac ccataatgta atagatctag cttatcgatg ataagctgtc     4020

aaagatgaga attaattcca cggactatag actataccta gtatactccg tctactgtac     4080

gatacacttc cgctcaggtc cttgtccttt aacgaggcct taccactctt ttgttactct     4140

attgatccag ctcagcaaag gcagtgtgat ctaagattct atcttcgcga tgtagtaaaa     4200

ctagctagac cgagaaagag actagaaatg caaaaggcac ttctacaatg gctgccatca     4260

ttattatccg atgtgacgct gcagcttctc aatgatattc gaatacgctt tgaggagata     4320

cagcctaata tccgacaaac tgttttacag atttacgatc gtacttgtta cccatcattg     4380

aattttgaac atccgaacct gggagttttc cctgaaacag atagtatatt tgaacctgta     4440

taataatata tagtctagcg ctttacggaa gacaatgtat gtatttcggt tcctggagaa     4500

actattgcat ctattgcata ggtaatcttg cacgtcgcat ccccggttca ttttctgcgt     4560

ttccatcttg cacttcaata gcatatcttt gttaacgaag catctgtgct tcattttgta     4620

gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt     4680

acagaacaga aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt     4740

ttgtaaaaca aaaatgcaac gcgacgagag cgctaatttt tcaaacaaag aatctgagct     4800

gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata     4860

cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt     4920

agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac     4980

tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa     5040

aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc     5100

aagataaagg catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac     5160

agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat     5220

tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact     5280

ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa     5340

aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta     5400

tatagggata tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa     5460

gcggtattcg caatgggaag ctccaccccg gttgataatc agaaaagccc caaaaacagg     5520

aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta     5580

aatttttgtt aaatcagctc attttttaac gaatagcccg aaatcggcaa aatcccttat     5640

aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttccaa caagagtcca     5700

ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa gggtctatca gggcgatggc     5760

ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcagta     5820

aatcggaagg gtaaacggat gcccccattt agagcttgac ggggaaagcc ggcgaacgtg     5880

gcgagaaagg aagggaagaa agcgaaagga gcgggggcta gggcggtggg aagtgtaggg     5940

gtcacgctgg gcgtaaccac cacacccgcc gcgcttaatg gggcgctaca gggcaggaat     6000

actctgaata aaacaactta tataataaaa atgc                                 6034


<210>  12
<211>  5056
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid


<220>
<221>  LV5
<222>  (1)..(40)

<220>
<221>  pYES backbone
<222>  (41)..(5016)

<220>
<221>  AmpR
<222>  (1040)..(1699)

<220>
<221>  URA3
<222>  (1915)..(3022)

<220>
<221>  LV3
<222>  (5017)..(5056)

<400>  12
cctctttata ttacatcaaa ataagaaaat aattataaca cctgcattaa tgaatcggcc       60

aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact      120

cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac      180

ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa      240

agcccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg      300

acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa      360

gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc      420

ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac      480

gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac      540

cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg      600

taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt      660

atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga      720

cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct      780

cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga      840

ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg      900

ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct      960

tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt     1020

aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc     1080

tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagc     1140

gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag     1200

atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt     1260

tatccgcctc cattcagtct attaattgtt gccgggaagc tagagtaagt agttcgccag     1320

ttaatagttt gcgcaacgtt gttggcattg ctacaggcat cgtggtgtca ctctcgtcgt     1380

ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca     1440

tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg     1500

ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat     1560

ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta     1620

tgcggcgacc gagttgctct tgcccggcgt caatacggga taatagtgta tcacatagca     1680

gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct     1740

taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat     1800

cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa     1860

agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatgggtaa     1920

taactgatat aattaaattg aagctctaat ttgtgagttt agtatacatg catttactta     1980

taatacagtt ttttagtttt gctggccgca tcttctcaaa tatgcttccc agcctgcttt     2040

tctgtaacgt tcaccctcta ccttagcatc ccttcccttt gcaaatagtc ctcttccaac     2100

aataataatg tcagatcctg tagagaccac atcatccacg gttctatact gttgacccaa     2160

tgcgtctccc ttgtcatcta aacccacacc gggtgtcata atcaaccaat cgtaaccttc     2220

atctcttcca cccatgtctc tttgagcaat aaagccgata acaaaatctt tgtcgctctt     2280

cgcaatgtca acagtaccct tagtatattc tccagtagat agggagccct tgcatgacaa     2340

ttctgctaac atcaaaaggc ctctaggttc ctttgttact tcttctgccg cctgcttcaa     2400

accgctaaca atacctgggc ccaccacacc gtgtgcattc gtaatgtctg cccattctgc     2460

tattctgtat acacccgcag agtactgcaa tttgactgta ttaccaatgt cagcaaattt     2520

tctgtcttcg aagagtaaaa aattgtactt ggcggataat gcctttagcg gcttaactgt     2580

gccctccatg gaaaaatcag tcaagatatc cacatgtgtt tttagtaaac aaattttggg     2640

acctaatgct tcaactaact ccagtaattc cttggtggta cgaacatcca atgaagcaca     2700

caagtttgtt tgcttttcgt gcatgatatt aaatagcttg gcagcaacag gactaggatg     2760

agtagcagca cgttccttat atgtagcttt cgacatgatt tatcttcgtt tcctgcaggt     2820

ttttgttctg tgcagttggg ttaagaatac tgggcaattt catgtttctt caacactaca     2880

tatgcgtata tataccaatc taagtctgtg ctccttcctt cgttcttcct tctgttcgga     2940

gattaccgaa tcaaaaaaat ttcaaagaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa     3000

tgatgaattg aattgaaaag ctagcttatc gatgataagc tgtcaaagat gagaattaat     3060

tccacggact atagactata ctagatactc cgtctactgt acgatacact tccgctcagg     3120

tccttgtcct ttaacgaggc cttaccactc ttttgttact ctattgatcc agctcagcaa     3180

aggcagtgtg atctaagatt ctatcttcgc gatgtagtaa aactagctag accgagaaag     3240

agactagaaa tgcaaaaggc acttctacaa tggctgccat cattattatc cgatgtgacg     3300

ctgcagcttc tcaatgatat tcgaatacgc tttgaggaga tacagcctaa tatccgacaa     3360

actgttttac agatttacga tcgtacttgt tacccatcat tgaattttga acatccgaac     3420

ctgggagttt tccctgaaac agatagtata tttgaacctg tataataata tatagtctag     3480

cgctttacgg aagacaatgt atgtatttcg gttcctggag aaactattgc atctattgca     3540

taggtaatct tgcacgtcgc atccccggtt cattttctgc gtttccatct tgcacttcaa     3600

tagcatatct ttgttaacga agcatctgtg cttcattttg tagaacaaaa atgcaacgcg     3660

agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac     3720

gcgaaagcgc tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca     3780

acgcgacgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga     3840

aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca     3900

aaaatgcatc ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc     3960

tttgtgcgct ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt     4020

agaagaaggc tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc     4080

cgcgtttact gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg     4140

attatattct ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga     4200

tgattcttca ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac     4260

gtataggaaa tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact     4320

acaatttttt tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt     4380

ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag     4440

agatatatag caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatggga     4500

agctccaccc cggttgataa tcagaaaagc cccaaaaaca ggaagattgt ataagcaaat     4560

atttaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc     4620

tcatttttta acgaatagcc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc     4680

gagatagggt tgagtgttgt tccagtttcc aacaagagtc cactattaaa gaacgtggac     4740

tccaacgtca aagggcgaaa aagggtctat cagggcgatg gcccactacg tgaaccatca     4800

ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcag taaatcggaa gggtaaacgg     4860

atgcccccat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag     4920

aaagcgaaag gagcgggggc tagggcggtg ggaagtgtag gggtcacgct gggcgtaacc     4980

accacacccg ccgcgcttaa tggggcgcta cagggcagga atactctgaa taaaacaact     5040

tatataataa aaatgc                                                     5056


<210>  13
<211>  10584
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cassette with Dictyostelium discoideum DiPKS coding sequence, 
       regulatory sequences and integration sequences


<220>
<221>  LV3
<222>  (1)..(40)

<220>
<221>  S. cerevisiae GAL1 promoter
<222>  (41)..(482)

<220>
<221>  L1
<222>  (483)..(522)

<220>
<221>  DiPKS
<222>  (523)..(9966)

<220>
<221>  Motif 1
<222>  (5050)..(5076)

<220>
<221>  C-methyltransferase domain
<222>  (5050)..(5412)

<220>
<221>  Motif 2
<222>  (5309)..(5331)

<220>
<221>  Motif 3
<222>  (5389)..(5421)

<220>
<221>  L2
<222>  (9967)..(10006)

<220>
<221>  PRM9t
<222>  (10007)..(10544)

<220>
<221>  LV5
<222>  (10545)..(10584)

<400>  13
aggaatactc tgaataaaac aacttatata ataaaaatgc cggattagaa gccgccgagc       60

gggtgacagc cctccgaagg aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt      120

tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa caataaagat tctacaatac      180

tagcttttat ggttatgaag aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa      240

tgaacgaatc aaattaacaa ccataggatg ataatgcgat tagtttttta gccttatttc      300

tggggtaatt aatcagcgaa gcgatgattt ttgatctatt aacagatata taaatgcaaa      360

aactgcataa ccactttaac taatactttc aacattttcg gtttgtatta cttcttattc      420

aaatgtaata aaagtatcaa caaaaaattg ttaatatacc tctatacttt aacgtcaagg      480

agctagaaaa tttattataa aaggaagaga aataattaaa caatgaacaa gaactccaaa      540

atccagtccc caaactcttc tgatgttgct gttattggtg ttggttttag attcccaggt      600

aactctaatg acccagaatc tttgtggaac aacttgttgg atggtttcga tgctattacc      660

caagtcccaa aagaaagatg ggctacttct tttagagaga tgggtttgat caagaacaag      720

ttcggtggtt tcttgaagga ttctgaatgg aagaatttcg accctttgtt ctttggtatc      780

ggtccaaaag aagctccatt cattgatcca caacaaaggt tgttgttgtc catcgtttgg      840

gaatctttgg aagatgctta catcagacca gatgaattga gaggttctaa cactggtgtt      900

ttcatcggtg tttctaacaa cgattacacc aagttgggtt tccaagacaa ctactctatt      960

tctccataca ctatgaccgg ctctaactct tcattgaact ccaacagaat ttcctactgc     1020

ttcgatttta gaggtccatc cattactgtt gataccgctt gttcttcttc cttggtttct     1080

gttaatttgg gtgtccaatc catccaaatg ggtgaatgta agattgctat ttgcggtggt     1140

gttaacgctt tgtttgatcc atctacatct gttgcctttt ccaagttggg tgttttgtct     1200

gaaaatggca gatgcaactc ttttagtgat caagcctctg gttacgttag atctgaaggt     1260

gctggtgttg ttgttttgaa gtctttggaa caagctaagt tggatggtga tagaatctac     1320

ggtgttatca agggtgtttc ctctaatgaa gatggtgctt ctaatggtga caagaactct     1380

ttgactactc catcttgtga agcccaatcc attaacattt ctaaggctat ggaaaaggcc     1440

tccttgtctc catctgatat ctattacatt gaagcccatg gtactggtac tccagttggt     1500

gatccaattg aagttaaggc cttgtccaag atcttctcca actctaacaa caaccagttg     1560

aacaacttct ctaccgatgg taatgataac gatgatgatg atgacgataa cacctctcca     1620

gaaccattat tgattggctc attcaagtcc aacatcggtc atttggaatc tgctgctggt     1680

attgcttctt tgattaagtg ttgcttgatg ttgaagaaca ggatgttggt tccatccatt     1740

aactgctcta atttgaaccc atccattcca ttcgatcagt acaacatctc cgttatcaga     1800

gaaatcagac aattcccaac cgataagttg gttaacatcg gtatcaattc tttcggtttc     1860

ggtggttcta actgccattt gattattcaa gagtacaaca acaacttcaa gaacaactct     1920

accatctgca ataacaacaa caacaacaat aacaacatcg actacttgat cccaatctcc     1980

tctaagacta agaagtcctt ggataagtac ttgattttga tcaagaccaa ctccaactac     2040

cacaaggata tttctttcga tgacttcgtc aagttccaaa tcaagtctaa gcagtacaac     2100

ttgtccaaca gaatgactac cattgctaac gattggaact ccttcattaa gggttctaac     2160

gaattccaca acttgatcga atctaaggat ggtgaaggtg gttcttcatc ttctaacaga     2220

ggtattgatt ccgccaatca aatcaacact actactacct ctaccatcaa cgatatcgaa     2280

cctttgttgg ttttcgtttt ctgtggtcaa ggtccacaat ggaatggtat gattaagacc     2340

ttgtacaact ccgagaacgt tttcaagaac accgttgatc atgttgacag catcttgtac     2400

aagtacttcg gttactccat tttgaacgtc ttgtctaaga tcgatgataa cgacgattcc     2460

atcaaccatc caatagttgc tcaaccatct ttgttcttgt tgcaaattgg tttggtcgag     2520

ttgtttaagt actggggtat ctacccatct atctctgttg gtcattcttt cggtgaagtc     2580

tcttcttatt acttgtccgg tatcatctct ttggaaaccg cttgtaaaat cgtctacgtc     2640

agatcctcta atcagaacaa aactatgggt tccggtaaga tgttggttgt ttctatgggt     2700

tttaagcaat ggaacgatca attctctgct gaatggtccg atattgaaat tgcttgttac     2760

aacgctccag attccatagt tgttactggt aacgaagaaa gattgaaaga attgtccatc     2820

aagttgtccg acgaatccaa tcaaattttc aacaccttct tgaggtcccc atgttctttt     2880

cattcttccc atcaagaagt catcaagggt tctatgttcg aagagttgtc taacttgcaa     2940

tctactggtg aaaccgaaat ccctttgttc tctactgtta ctggtagaca agttttgtct     3000

ggtcatgtta ctgctcaaca catctacgat aatgttagag aaccagtctt gttccaaaag     3060

acgattgaat ccattacctc ctacatcaag tctcactacc catccaatca aaaggttatc     3120

tacgttgaaa ttgctccaca cccaaccttg ttttcattga tcaaaaagtc catcccatcc     3180

tccaacaaga attcctcttc tgttttgtgt ccattgaaca gaaaagaaaa ctccaacaac     3240

tcctacaaga agttcgtttc tcagttgtac ttcaacggtg ttaacgttga cttcaacttc     3300

cagttgaact ccatttgcga taacgttaac aacgatcacc atttgaacaa cgtcaagcaa     3360

aactccttca aagagactac caattccttg ccaagatacc aatgggaaca agatgaatat     3420

tggtccgaac cattgatctc cagaaagaat agattggaag gtccaactac ttccttgttg     3480

ggtcatagaa ttatctacag cttcccagtt ttccaatccg ttttggactt gcaatctgac     3540

aactacaaat acttgttgga ccacttggtt aacggtaagc cagtttttcc aggtgctggt     3600

tatttggata tcatcatcga attcttcgac taccaaaagc agcagttgaa ttcctctgat     3660

tcctctaact cctacatcat caacgttgac aagatccaat tcttgaaccc aattcacttg     3720

accgaaaaca agttgcaaac cttgcaatct tctttcgaac ctatcgttac taagaagtct     3780

gccttctctg ttaacttctt catcaaggat accgtcgagg atcaatctaa ggttaagtct     3840

atgtctgacg aaacttggac taacacttgt aaggctacca tttccttgga acaacaacag     3900

ccatctccat cttctacttt gactttgtct aagaagcaag acttgcagat cttgagaaac     3960

agatgcgata ttagcaagct agacaagttt gagttgtacg acaagatctc taagaatttg     4020

ggcttgcagt acaactcctt gtttcaagtt gttgatacca tcgaaactgg taaggattgc     4080

tcttttgcta ctttgtcttt gccagaagat actttgttca ccaccatttt gaacccatgc     4140

ttgttggata actgtttcca tggtttgttg accttgatca acgaaaaggg ttctttcgtt     4200

gtcgagtcca tttcttctgt ttctatctac ttggagaaca tcggttcctt caatcaaact     4260

tctgttggta acgtccagtt ctacttgtac accactattt ctaaagccac ctcctttagt     4320

tctgaaggta cttgtaagtt gttcaccaag gatggttcct tgattttgtc tatcggtaag     4380

ttcatcatca agtccaccaa tccaaagtct actaagacca acgaaactat cgaatctcca     4440

ttggacgaaa ccttctctat tgaatggcaa tctaaggatt ctccaattcc aaccccacaa     4500

caaatccaac aacaatctcc attgaactct aacccatcct tcattagatc taccatcttg     4560

aaggacatcc agttcgaaca atactgctcc tccattatcc acaaagaatt gatcaaccac     4620

gaaaagtaca agaaccagca atccttcgat atcaactcct tggaaaacca cttgaacgat     4680

gaccaattga tggaatcctt gtccatctcc aaagaatact tgagattctt caccaggatc     4740

atctccatca ttaagcaata cccaaagatc ttgaacgaaa aagagctaaa agaattgaaa     4800

gaaatcatcg aattgaagta cccatccgaa gttcagttgt tggaattcga agttatcgag     4860

aaggtgtcca tgattatccc aaagttgttg ttcgaaaacg acaagcaatc ttccatgacc     4920

ttgttccaag ataacttgtt gaccaggttc tactccaatt ctaactctac cagattctac     4980

ttggaaaggg tttccgaaat ggtcttggaa tctattagac caatcgtcag agaaaagagg     5040

gtgttcagaa ttttggaaat tggtgctggt acaggctctt tgtctaatgt tgttttgact     5100

aagttgaaca cctacttgtc caccttgaat tctaatggtg gttctggtta caacatcatc     5160

attgagtaca ccttcaccga tatttccgcc aacttcatta ttggtgaaat ccaagaaacc     5220

atgtgcaact tgtacccaaa cgttactttc aagttctccg tcttggactt ggagaaagag     5280

attattaact cctccgattt cttgatgggt gattacgata tagttttgat ggcctacgtt     5340

atccatgccg tttctaacat taagttctcc atcgaacagt tgtacaagtt gttgtctcca     5400

agaggttggt tgttgtgtat tgaacctaag tccaacgttg tgttctccga tttggttttc     5460

ggttgtttta atcagtggtg gaactactac gatgatatta gaactaccca ctgctccttg     5520

tctgaatctc aatggaatca gttgttgttg aaccagtcct tgaacaacga atcctcttct     5580

tcttctaact gttacggtgg tttctccaac gtttctttta ttggtggtga aaaggatgtc     5640

gactcccatt ctttcatatt gcactgccaa aaagaatcca tctcccaaat gaagttagcc     5700

accactatta acaacggttt gtcatctggt tccatcgtta tcgttttgaa ctctcaacaa     5760

ttgaccaaca tgaagtccta cccaaaggtt attgagtata ttcaagaggc tacctctttg     5820

tgcaagacca ttgaaattat cgattccaag gacgtcttga actctaccaa ttcagttttg     5880

gaaaagatcc aaaagtcctt gttggtgttc tgtttgttgg gttatgactt gttggagaac     5940

aactaccaag aacagtcttt cgaatacgtt aagttgttga acttgatctc tactaccgcc     6000

tcttcatcta atgataagaa accaccaaag gtcttgttga tcaccaagca atctgaaaga     6060

atctccaggt ctttctactc cagatccttg attggtattt ccagaacctc tatgaacgag     6120

tacccaaatt tgtccattac ctctatcgat ttggatacca acgactactc attgcagtct     6180

ttgttgaagc caatcttcag caactctaag ttttccgaca acgagttcat cttcaaaaag     6240

ggcttgatgt tcgtgtccag gatctttaag aacaagcagt tgctagaatc ctccaacgct     6300

tttgaaactg actcttctaa cttgtactgt aaggcctctt ctgacttgtc ttacaagtac     6360

gctattaagc agtctatgtt gaccgaaaat cagatcgaaa tcaaggttga atgcgtcggt     6420

attaacttca aggacaacct attctacaag ggcttgttgc cacaagaaat tttcagaatg     6480

ggtgacatct acaatccacc atatggtttg gaatgctctg gtgttattac cagaattggt     6540

tctaacgtca ccgaatactc agttggtcaa aatgtttttg gtttcgccag acattctttg     6600

ggttctcatg ttgttaccaa caaggatttg gttatcttga agccagatac catctcattt     6660

tctgaagctg cttctatccc agttgtttac tgtactgctt ggtactcctt gttcaacatt     6720

ggtcagttgt ctaacgaaga atccatccta attcattctg ctactggtgg tgtaggtttg     6780

gcttctttga atttgttgaa aatgaagaat cagcaacagc aaccattgac caatgtttat     6840

gctactgttg gctctaacga gaagaagaag ttcttgatcg ataacttcaa caacttgttc     6900

aaagaggacg gcgaaaacat tttctctacc agagacaaag aatactccaa ccagttggaa     6960

tccaagatcg atgttatttt gaacaccttg tccggtgaat tcgtcgaatc taatttcaag     7020

tccttgagat ccttcggtag attgattgat ttgtctgcta ctcacgttta cgccaatcaa     7080

caaattggtc taggtaactt caagttcgac cacttgtatt ctgctgttga cttggaaaga     7140

ttgatcgacg aaaaacctaa gttgttgcag tccatcttgc aaagaattac caactctatc     7200

gtcaacggtt ccttggaaaa aattccaatt accatcttcc catccaccga aactaaggat     7260

gctatcgaat tattgtccaa gagatcccat atcggtaaag ttgttgtaga ttgcaccgat     7320

atctctaagt gtaatcctgt tggtgatgtg atcaccaact tctctatgag attgccaaag     7380

ccaaactacc agttgaattt gaactccacc ttgttgatta ctggtcagtc tggtttgtct     7440

atccctttgt tgaattggtt gttgtctaag tctggtggta acgttaagaa cgttgtcatc     7500

atttctaagt ccaccatgaa gtggaagttg cagactatga tttcccattt cgtttccggt     7560

ttcggtatcc attttaacta cgttcaagtc gacatctcca actacgatgc tttgtctgaa     7620

gctattaagc aattgccatc tgatttgcca ccaatcacct ctgtttttca tttggctgct     7680

atctacaacg atgttccaat ggatcaagtt accatgtcta ccgttgaatc tgttcataac     7740

cctaaagttt tgggtgccgt taacttgcat agaatctctg tttcttttgg ttggaagttg     7800

aaccacttcg tcttgttctc ttctattact gctattaccg gttacccaga ccaatctatc     7860

tacaattctg ccaactctat tttggacgct ttgtccaact ttagaaggtt tatgggtttg     7920

ccatccttct ccattaactt gggtccaatg aaggatgaag gtaaggtttc taccaacaag     7980

agcatcaaga agctattcaa gtctagaggt ttgccaagcc tatccttgaa caagttattt     8040

ggtttgttgg aggtcgtcat caacaaccca tctaatcatg ttatcccatc ccaattgatt     8100

tgctccccaa tcgatttcaa gacctacatc gaatctttct caactatgag gccaaagttg     8160

ttacacttgc aacctaccat ttccaagcag caatcttcta tcattaacga ttctaccaag     8220

gcttcctcca acatttcatt gcaagataag atcacctcca aggtgtctga tttgttgtcc     8280

attccaatct ccaagatcaa cttcgatcat ccattgaaac actacggctt ggattctttg     8340

ttgaccgttc aattcaaatc ctggatcgac aaagaattcg aaaagaactt gttcacccat     8400

atccaattgg ccaccatctc tattaactca ttcttggaaa aggtgaacgg cttgtctaca     8460

aacaataaca acaacaacaa ttccaacgtc aagtcctctc catccattgt caaagaagaa     8520

atcgttacct tggacaagga tcaacaacca ttgctattga aagaacacca gcacattatc     8580

atctccccag atattagaat caacaagcca aagagggaat ccttgattag aaccccaatc     8640

ttgaacaaat tcaaccagat caccgaatcc attatcactc catctacacc atctttgtcc     8700

caatccgatg ttttgaaaac tccaccaatc aagtctttga acaacactaa gaactccagc     8760

ttgattaaca ccccaccaat tcaatctgtc caacaacatc aaaagcaaca acaaaaggtc     8820

caagtcatcc aacaacagca acaaccatta tccagattgt cctacaagag caacaacaac     8880

tctttcgttt tgggtatcgg tatttctgtt ccaggtgaac ctatttccca acaatccttg     8940

aaagactcca tctccaatga cttttctgat aaggctgaaa ctaacgagaa ggtcaagaga     9000

atctttgagc aatctcaaat caagaccaga cacttggtta gagattacac taagccagag     9060

aactccatca agttcagaca tttggaaacc attaccgatg tgaacaacca gttcaagaaa     9120

gttgttccag atttggctca acaagcctgt ttgagagctt tgaaagattg gggtggtgat     9180

aagggtgata ttacccatat agtttctgtt acctccaccg gtattatcat cccagatgtt     9240

aatttcaagt tgatcgactt gttgggcttg aacaaggatg ttgaaagagt gtctttgaac     9300

ctaatgggtt gtttggctgg tttgagttct ttgagaactg ctgcttcttt ggctaaggct     9360

tctccaagaa atagaatttt ggttgtctgt accgaagtct gctccttgca tttttctaat     9420

actgatggtg gtgatcaaat ggtcgcctct tctatttttg ctgatggttc tgctgcttac     9480

attattggtt gtaacccaag aattgaagaa accccattat acgaagtcat gtgctccatt     9540

aacagatctt tcccaaatac cgaaaacgcc atggtttggg atttggaaaa agaaggttgg     9600

aacttgggtt tggatgcttc tattccaatt gtcattggtt ctggtattga agccttcgtt     9660

gatactttgt tggataaggc taagttgcaa acttccactg ctatttctgc taaggattgc     9720

gaattcttga ttcatactgg tggcaagtcc atcttgatga acatcgaaaa ttccttgggt     9780

atcgacccaa agcaaactaa gaatacttgg gatgtttacc atgcctacgg caatatgtca     9840

tctgcctctg ttattttcgt tatggatcat gccagaaagt ccaagtcttt gccaacttac     9900

tcaatttctt tggcttttgg tccaggtttg gcttttgaag gttgtttctt gaagaacgtc     9960

gtctaaagac ataaaactga aacaacacca attaataata gactttacag aagacgggag    10020

acactagcac acaactttac caggcaaggt atttgacgct agcatgtgtc caattcagtg    10080

tcatttatga ttttttgtag taggatataa atatatacag cgctccaaat agtgcggttg    10140

ccccaaaaac accacggaac ctcatctgtt ctcgtacttt gttgtgacaa agtagctcac    10200

tgccttatta tcacattttc attatgcaac gcttcggaaa atacgatgtt gaaaatgcct    10260

ctagagatga aaaacaatcg taaaagggtc ctgcgtaatt gaaacatttg atcagtatgc    10320

agtggcacag aaacaaccag gaatactata gtcataggca atacaaggta tatattggct    10380

atgcagaccc ctccagaaag taccgacgtc aagttagata cacttaacga acctagtgca    10440

catttaattg agaaaaatgt ggctcttcct aaggacatat tccgttcgta cttgagttat    10500

tggatctatg aaatcgctcg ctatacacca gtcatgattt tgtccctctt tatattacat    10560

caaaataaga aaataattat aaca                                           10584


<210>  14
<211>  4909
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cassette with Cas9 coding sequence, regulatory sequences and 
       integration sequences


<220>
<221>  LV3
<222>  (1)..(40)

<220>
<221>  TEF1p
<222>  (41)..(446)

<220>
<221>  Cas9
<222>  (470)..(4609)

<220>
<221>  LV5
<222>  (4870)..(4909)

<400>  14
aggaatactc tgaataaaac aacttatata ataaaaatgc atagcttcaa aatgtttcta       60

ctcctttttt actcttccag attttctcgg actccgcgca tcgccgtacc acttcaaaac      120

acccaagcac agcatactaa atttcccctc tttcttcctc tagggtgtcg ttaattaccc      180

gtactaaagg tttggaaaag aaaaaagaga ccgcctcgtt tctttttctt cgtcgaaaaa      240

ggcaataaaa atttttatca cgtttctttt tcttgaaaat tttttttttg atttttttct      300

ctttcgatga cctcccattg atatttaagt taataaacgg tcttcaattt ctcaagtttc      360

agtttcattt ttcttgttct attacaactt tttttacttc ttgctcatta gaaagaaagc      420

atagcaatct aatctaagtt ttctagaact agtggatccc ccgggaaaaa tggacaagaa      480

gtactccatt gggctcgata tcggcacaaa cagcgtcggc tgggccgtca ttacggacga      540

gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc acagcataaa      600

gaagaacctc attggcgccc tcctgttcga ctccggggag acggccgaag ccacgcggct      660

caaaagaaca gcacggcgca gatatacccg cagaaagaat cggatctgct acctgcagga      720

gatctttagt aatgagatgg ctaaggtgga tgactctttc ttccataggc tggaggagtc      780

ctttttggtg gaggaggata aaaagcacga gcgccaccca atctttggca atatcgtgga      840

cgaggtggcg taccatgaaa agtacccaac catatatcat ctgaggaaga agcttgtaga      900

cagtactgat aaggctgact tgcggttgat ctatctcgcg ctggcgcata tgatcaaatt      960

tcggggacac ttcctcatcg agggggacct gaacccagac aacagcgatg tcgacaaact     1020

ctttatccaa ctggttcaga cttacaatca gcttttcgaa gagaacccga tcaacgcatc     1080

cggagttgac gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc ggctcgaaaa     1140

cctcatcgca cagctccctg gggagaagaa gaacggcctg tttggtaatc ttatcgccct     1200

gtcactcggg ctgaccccca actttaaatc taacttcgac ctggccgaag atgccaagct     1260

tcaactgagc aaagacacct acgatgatga tctcgacaat ctgctggccc agatcggcga     1320

ccagtacgca gacctttttt tggcggcaaa gaacctgtca gacgccattc tgctgagtga     1380

tattctgcga gtgaacacgg agatcaccaa agctccgctg agcgctagta tgatcaagcg     1440

ctatgatgag caccaccaag acttgacttt gctgaaggcc cttgtcagac agcaactgcc     1500

tgagaagtac aaggaaattt tcttcgatca gtctaaaaat ggctacgccg gatacattga     1560

cggcggagca agccaggagg aattttacaa atttattaag cccatcttgg aaaaaatgga     1620

cggcaccgag gagctgctgg taaagcttaa cagagaagat ctgttgcgca aacagcgcac     1680

tttcgacaat ggaagcatcc cccaccagat tcacctgggc gaactgcacg ctatcctcag     1740

gcggcaagag gatttctacc cctttttgaa agataacagg gaaaagattg agaaaatcct     1800

cacatttcgg ataccctact atgtaggccc cctcgcccgg ggaaattcca gattcgcgtg     1860

gatgactcgc aaatcagaag agaccatcac tccctggaac ttcgaggaag tcgtggataa     1920

gggggcctct gcccagtcct tcatcgaaag gatgactaac tttgataaaa atctgcctaa     1980

cgaaaaggtg cttcctaaac actctctgct gtacgagtac ttcacagttt ataacgagct     2040

caccaaggtc aaatacgtca cagaagggat gagaaagcca gcattcctgt ctggagagca     2100

gaagaaagct atcgtggacc tcctcttcaa gacgaaccgg aaagttaccg tgaaacagct     2160

caaagaagac tatttcaaaa agattgaatg tttcgactct gttgaaatca gcggagtgga     2220

ggatcgcttc aacgcatccc tgggaacgta tcacgatctc ctgaaaatca ttaaagacaa     2280

ggacttcctg gacaatgagg agaacgagga cattcttgag gacattgtcc tcacccttac     2340

gttgtttgaa gatagggaga tgattgaaga acgcttgaaa acttacgctc atctcttcga     2400

cgacaaagtc atgaaacagc tcaagaggcg ccgatataca ggatgggggc ggctgtcaag     2460

aaaactgatc aatgggatcc gagacaagca gagtggaaag acaatcctgg attttcttaa     2520

gtccgatgga tttgccaacc ggaacttcat gcagttgatc catgatgact ctctcacctt     2580

taaggaggac atccagaaag cacaagtttc tggccagggg gacagtcttc acgagcacat     2640

cgctaatctt gcaggtagcc cagctatcaa aaagggaata ctgcagaccg ttaaggtcgt     2700

ggatgaactc gtcaaagtaa tgggaaggca taagcccgag aatatcgtta tcgagatggc     2760

ccgagagaac caaactaccc agaagggaca gaagaacagt agggaaagga tgaagaggat     2820

tgaagagggt ataaaagaac tggggtccca aatccttaag gaacacccag ttgaaaacac     2880

ccagcttcag aatgagaagc tctacctgta ctacctgcag aacggcaggg acatgtacgt     2940

ggatcaggaa ctggacatca atcggctctc cgactacgac gtggatcata tcgtgcccca     3000

gtcttttctc aaagatgatt ctattgataa taaagtgttg acaagatccg ataaaaatag     3060

agggaagagt gataacgtcc cctcagaaga agttgtcaag aaaatgaaaa attattggcg     3120

gcagctgctg aacgccaaac tgatcacaca acggaagttc gataatctga ctaaggctga     3180

acgaggtggc ctgtctgagt tggataaagc cggcttcatc aaaaggcagc ttgttgagac     3240

acgccagatc accaagcacg tggcccaaat tctcgattca cgcatgaaca ccaagtacga     3300

tgaaaatgac aaactgattc gagaggtgaa agttattact ctgaagtcta agctggtctc     3360

agatttcaga aaggactttc agttttataa ggtgagagag atcaacaatt accaccatgc     3420

gcatgatgcc tacctgaatg cagtggtagg cactgcactt atcaaaaaat atcccaagct     3480

tgaatctgaa tttgtttacg gagactataa agtgtacgat gttaggaaaa tgatcgcaaa     3540

gtctgagcag gaaataggca aggccaccgc taagtacttc ttttacagca atattatgaa     3600

ttttttcaag accgagatta cactggccaa tggagagatt cggaagcgac cacttatcga     3660

aacaaacgga gaaacaggag aaatcgtgtg ggacaagggt agggatttcg cgacagtccg     3720

gaaggtcctg tccatgccgc aggtgaacat cgttaaaaag accgaagtac agaccggagg     3780

cttctccaag gaaagtatcc tcccgaaaag gaacagcgac aagctgatcg cacgcaaaaa     3840

agattgggac cccaagaaat acggcggatt cgattctcct acagtcgctt acagtgtact     3900

ggttgtggcc aaagtggaga aagggaagtc taaaaaactc aaaagcgtca aggaactgct     3960

gggcatcaca atcatggagc gatcaagctt cgaaaaaaac cccatcgact ttctcgaggc     4020

gaaaggatat aaagaggtca aaaaagacct catcattaag cttcccaagt actctctctt     4080

tgagcttgaa aacggccgga aacgaatgct cgctagtgcg ggcgagctgc agaaaggtaa     4140

cgagctggca ctgccctcta aatacgttaa tttcttgtat ctggccagcc actatgaaaa     4200

gctcaaaggg tctcccgaag ataatgagca gaagcagctg ttcgtggaac aacacaaaca     4260

ctaccttgat gagatcatcg agcaaataag cgaattctcc aaaagagtga tcctcgccga     4320

cgctaacctc gataaggtgc tttctgctta caataagcac agggataagc ccatcaggga     4380

gcaggcagaa aacattatcc acttgtttac tctgaccaac ttgggcgcgc ctgcagcctt     4440

caagtacttc gacaccacca tagacagaaa gcggtacacc tctacaaagg aggtcctgga     4500

cgccacactg attcatcagt caattacggg gctctatgaa acaagaatcg acctctctca     4560

gctcggtgga gacagcaggg ctgaccccaa gaagaagagg aaggtgtgat ctcttctcga     4620

gtcatgtaat tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc     4680

gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg     4740

ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg     4800

tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct     4860

ttaatttgcc ctctttatat tacatcaaaa taagaaaata attataaca                 4909


