                         SEQUENCE LISTING

<110>  The University of Manchester
 
<120>  GENE THERAPY

<130>  P33752WO/SNB

<150>  GB1701968.8
<151>  2017-02-07

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  1650
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  coIDS DNA sequence

<400>  1
atgcctccac ctagaactgg aagaggcctg ctgtggctgg gcctggtgct gtctagtgtg       60

tgtgtggccc tgggcagcga gacacaggcc aacagcacaa ccgacgccct gaacgtgctg      120

ctgatcatcg tggacgacct gaggcctagc ctgggctgct acggcgataa gctcgtgcgg      180

agccccaaca tcgatcagct ggcctctcac agcctgctgt tccagaacgc attcgcacag      240

caggcagtgt gcgcccccag cagagtgtct ttcctgaccg gcagaaggcc cgacaccacc      300

cggctgtacg acttcaacag ctactggcgg gtgcacgccg gcaacttcag caccatcccc      360

cagtacttca aagaaaacgg ctacgtgacc atgagcgtgg gcaaggtgtt ccaccccggc      420

atcagctcca accacaccga cgacagcccc tacagctggt ccttcccacc ctaccacccc      480

agcagcgaga agtacgagaa caccaagacc tgcagaggcc ccgacggcga gctgcatgcc      540

aatctgctgt gccccgtgga cgtgctggat gtgcctgagg gaaccctgcc cgacaagcag      600

tctaccgagc aggccattca gctgctggaa aagatgaaga ccagcgccag ccccttcttc      660

ctggccgtgg gctatcacaa gccccacatc cctttcagat accccaaaga gttccagaag      720

ctgtaccccc tggaaaacat caccctggcc cccgatcctg aggtgccaga tggactgcct      780

cccgtggcct acaacccctg gatggacatc cggcagcgcg aggatgtgca ggccctgaat      840

atcagcgtgc cctacggccc catccccgtg gatttccagc ggaagatccg gcagagctac      900

ttcgccagcg tgtcctacct ggacacccaa gtgggcagac tgctgagcgc cctggacgat      960

ctgcagctgg ccaactccac catcattgcc ttcaccagcg accacggctg ggccctggga     1020

gaacatggcg agtgggccaa gtacagcaac ttcgacgtgg ccacccacgt gcccctgatc     1080

ttctacgtgc caggcagaac cgccagcctg cctgaggctg gcgagaagct gttcccttac     1140

ctggacccct tcgacagcgc ctcccagctg atggaacctg gcagacagag catggacctg     1200

gtggaactgg tgtccctgtt ccccacactg gccggactgg ctggactgca ggtgccccct     1260

agatgtcccg tgcctagctt tcacgtggaa ctgtgcagag agggcaagaa cctgctgaag     1320

cacttccggt tccgggacct ggaagaagat ccctacctgc ccggcaaccc cagagagctg     1380

atcgcctaca gccagtaccc cagacccagc gacatccctc agtggaacag cgacaagccc     1440

agcctgaagg acatcaagat catgggctac agcatccgga ccatcgacta ccggtacacc     1500

gtgtgggtgg gattcaaccc cgacgagttc ctggccaatt tctccgacat ccacgccggg     1560

gagctgtact tcgtggacag cgatcccctg caggaccaca acatgtacaa cgacagccag     1620

ggcggcgacc tgttccagct gctgatgccc                                      1650


<210>  2
<211>  1650
<212>  DNA
<213>  Homo sapiens

<400>  2
atgccgccac cccggaccgg ccgaggcctt ctctggctgg gtctggttct gagctccgtc       60

tgcgtcgccc tcggatccga aacgcaggcc aactcgacca cagatgctct gaacgttctt      120

ctcatcatcg tggatgacct gcgcccctcc ctgggctgtt atggggataa gctggtgagg      180

tccccaaata ttgaccaact ggcatcccac agcctcctct tccagaatgc ctttgcgcag      240

caagcagtgt gcgccccgag ccgcgtttct ttcctcactg gcaggagacc tgacaccacc      300

cgcctgtacg acttcaactc ctactggagg gtgcacgctg gaaacttctc caccatcccc      360

cagtacttca aggagaatgg ctatgtgacc atgtcggtgg gaaaagtctt tcaccctggg      420

atatcttcta accataccga tgattctccg tatagctggt cttttccacc ttatcatcct      480

tcctctgaga agtatgaaaa cactaagaca tgtcgagggc cagatggaga actccatgcc      540

aacctgcttt gccctgtgga tgtgctggat gttcccgagg gcaccttgcc tgacaaacag      600

agcactgagc aagccataca gttgttggaa aagatgaaaa cgtcagccag tcctttcttc      660

ctggccgttg ggtatcataa gccacacatc cccttcagat accccaagga atttcagaag      720

ttgtatccct tggagaacat caccctggcc cccgatcccg aggtccctga tggcctaccc      780

cctgtggcct acaacccctg gatggacatc aggcaacggg aagacgtcca agccttaaac      840

atcagtgtgc cgtatggtcc aattcctgtg gactttcagc ggaaaatccg ccagagctac      900

tttgcctctg tgtcatattt ggatacacag gtcggccgcc tcttgagtgc tttggacgat      960

cttcagctgg ccaacagcac catcattgca tttacctcgg atcatgggtg ggctctaggt     1020

gaacatggag aatgggccaa atacagcaat tttgatgttg ctacccatgt tcccctgata     1080

ttctatgttc ctggaaggac ggcttcactt ccggaggcag gcgagaagct tttcccttac     1140

ctcgaccctt ttgattccgc ctcacagttg atggagccag gcaggcaatc catggacctt     1200

gtggaacttg tgtctctttt tcccacgctg gctggacttg caggactgca ggttccacct     1260

cgctgccccg ttccttcatt tcacgttgag ctgtgcagag aaggcaagaa ccttctgaag     1320

cattttcgat tccgtgactt ggaagaggat ccgtacctcc ctggtaatcc ccgtgaactg     1380

attgcctata gccagtatcc ccggccttca gacatccctc agtggaattc tgacaagccg     1440

agtttaaaag atataaagat catgggctat tccatacgca ccatagacta taggtatact     1500

gtgtgggttg gcttcaatcc tgatgaattt ctagctaact tttctgacat ccatgcaggg     1560

gaactgtatt ttgtggattc tgacccattg caggatcaca atatgtataa tgattcccaa     1620

ggtggagatc ttttccagtt gttgatgcct                                      1650


<210>  3
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ApoE tandem repeat (ApoEII)

<400>  3
ctgagaaagc tgcggaagcg gctgctgctg aggaagctga gaaaaagact gctg             54


<210>  4
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  4
ctgggagggg gaggatctgg cggaggcgga agtggcggcg gaggatcagg gggcggaggc       60

tct                                                                     63


<210>  5
<211>  550
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  coIDS

<400>  5

Met Pro Pro Pro Arg Thr Gly Arg Gly Leu Leu Trp Leu Gly Leu Val 
1               5                   10                  15      


Leu Ser Ser Val Cys Val Ala Leu Gly Ser Glu Thr Gln Ala Asn Ser 
            20                  25                  30          


Thr Thr Asp Ala Leu Asn Val Leu Leu Ile Ile Val Asp Asp Leu Arg 
        35                  40                  45              


Pro Ser Leu Gly Cys Tyr Gly Asp Lys Leu Val Arg Ser Pro Asn Ile 
    50                  55                  60                  


Asp Gln Leu Ala Ser His Ser Leu Leu Phe Gln Asn Ala Phe Ala Gln 
65                  70                  75                  80  


Gln Ala Val Cys Ala Pro Ser Arg Val Ser Phe Leu Thr Gly Arg Arg 
                85                  90                  95      


Pro Asp Thr Thr Arg Leu Tyr Asp Phe Asn Ser Tyr Trp Arg Val His 
            100                 105                 110         


Ala Gly Asn Phe Ser Thr Ile Pro Gln Tyr Phe Lys Glu Asn Gly Tyr 
        115                 120                 125             


Val Thr Met Ser Val Gly Lys Val Phe His Pro Gly Ile Ser Ser Asn 
    130                 135                 140                 


His Thr Asp Asp Ser Pro Tyr Ser Trp Ser Phe Pro Pro Tyr His Pro 
145                 150                 155                 160 


Ser Ser Glu Lys Tyr Glu Asn Thr Lys Thr Cys Arg Gly Pro Asp Gly 
                165                 170                 175     


Glu Leu His Ala Asn Leu Leu Cys Pro Val Asp Val Leu Asp Val Pro 
            180                 185                 190         


Glu Gly Thr Leu Pro Asp Lys Gln Ser Thr Glu Gln Ala Ile Gln Leu 
        195                 200                 205             


Leu Glu Lys Met Lys Thr Ser Ala Ser Pro Phe Phe Leu Ala Val Gly 
    210                 215                 220                 


Tyr His Lys Pro His Ile Pro Phe Arg Tyr Pro Lys Glu Phe Gln Lys 
225                 230                 235                 240 


Leu Tyr Pro Leu Glu Asn Ile Thr Leu Ala Pro Asp Pro Glu Val Pro 
                245                 250                 255     


Asp Gly Leu Pro Pro Val Ala Tyr Asn Pro Trp Met Asp Ile Arg Gln 
            260                 265                 270         


Arg Glu Asp Val Gln Ala Leu Asn Ile Ser Val Pro Tyr Gly Pro Ile 
        275                 280                 285             


Pro Val Asp Phe Gln Arg Lys Ile Arg Gln Ser Tyr Phe Ala Ser Val 
    290                 295                 300                 


Ser Tyr Leu Asp Thr Gln Val Gly Arg Leu Leu Ser Ala Leu Asp Asp 
305                 310                 315                 320 


Leu Gln Leu Ala Asn Ser Thr Ile Ile Ala Phe Thr Ser Asp His Gly 
                325                 330                 335     


Trp Ala Leu Gly Glu His Gly Glu Trp Ala Lys Tyr Ser Asn Phe Asp 
            340                 345                 350         


Val Ala Thr His Val Pro Leu Ile Phe Tyr Val Pro Gly Arg Thr Ala 
        355                 360                 365             


Ser Leu Pro Glu Ala Gly Glu Lys Leu Phe Pro Tyr Leu Asp Pro Phe 
    370                 375                 380                 


Asp Ser Ala Ser Gln Leu Met Glu Pro Gly Arg Gln Ser Met Asp Leu 
385                 390                 395                 400 


Val Glu Leu Val Ser Leu Phe Pro Thr Leu Ala Gly Leu Ala Gly Leu 
                405                 410                 415     


Gln Val Pro Pro Arg Cys Pro Val Pro Ser Phe His Val Glu Leu Cys 
            420                 425                 430         


Arg Glu Gly Lys Asn Leu Leu Lys His Phe Arg Phe Arg Asp Leu Glu 
        435                 440                 445             


Glu Asp Pro Tyr Leu Pro Gly Asn Pro Arg Glu Leu Ile Ala Tyr Ser 
    450                 455                 460                 


Gln Tyr Pro Arg Pro Ser Asp Ile Pro Gln Trp Asn Ser Asp Lys Pro 
465                 470                 475                 480 


Ser Leu Lys Asp Ile Lys Ile Met Gly Tyr Ser Ile Arg Thr Ile Asp 
                485                 490                 495     


Tyr Arg Tyr Thr Val Trp Val Gly Phe Asn Pro Asp Glu Phe Leu Ala 
            500                 505                 510         


Asn Phe Ser Asp Ile His Ala Gly Glu Leu Tyr Phe Val Asp Ser Asp 
        515                 520                 525             


Pro Leu Gln Asp His Asn Met Tyr Asn Asp Ser Gln Gly Gly Asp Leu 
    530                 535                 540                 


Phe Gln Leu Leu Met Pro 
545                 550 


<210>  6
<211>  550
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Pro Pro Pro Arg Thr Gly Arg Gly Leu Leu Trp Leu Gly Leu Val 
1               5                   10                  15      


Leu Ser Ser Val Cys Val Ala Leu Gly Ser Glu Thr Gln Ala Asn Ser 
            20                  25                  30          


Thr Thr Asp Ala Leu Asn Val Leu Leu Ile Ile Val Asp Asp Leu Arg 
        35                  40                  45              


Pro Ser Leu Gly Cys Tyr Gly Asp Lys Leu Val Arg Ser Pro Asn Ile 
    50                  55                  60                  


Asp Gln Leu Ala Ser His Ser Leu Leu Phe Gln Asn Ala Phe Ala Gln 
65                  70                  75                  80  


Gln Ala Val Cys Ala Pro Ser Arg Val Ser Phe Leu Thr Gly Arg Arg 
                85                  90                  95      


Pro Asp Thr Thr Arg Leu Tyr Asp Phe Asn Ser Tyr Trp Arg Val His 
            100                 105                 110         


Ala Gly Asn Phe Ser Thr Ile Pro Gln Tyr Phe Lys Glu Asn Gly Tyr 
        115                 120                 125             


Val Thr Met Ser Val Gly Lys Val Phe His Pro Gly Ile Ser Ser Asn 
    130                 135                 140                 


His Thr Asp Asp Ser Pro Tyr Ser Trp Ser Phe Pro Pro Tyr His Pro 
145                 150                 155                 160 


Ser Ser Glu Lys Tyr Glu Asn Thr Lys Thr Cys Arg Gly Pro Asp Gly 
                165                 170                 175     


Glu Leu His Ala Asn Leu Leu Cys Pro Val Asp Val Leu Asp Val Pro 
            180                 185                 190         


Glu Gly Thr Leu Pro Asp Lys Gln Ser Thr Glu Gln Ala Ile Gln Leu 
        195                 200                 205             


Leu Glu Lys Met Lys Thr Ser Ala Ser Pro Phe Phe Leu Ala Val Gly 
    210                 215                 220                 


Tyr His Lys Pro His Ile Pro Phe Arg Tyr Pro Lys Glu Phe Gln Lys 
225                 230                 235                 240 


Leu Tyr Pro Leu Glu Asn Ile Thr Leu Ala Pro Asp Pro Glu Val Pro 
                245                 250                 255     


Asp Gly Leu Pro Pro Val Ala Tyr Asn Pro Trp Met Asp Ile Arg Gln 
            260                 265                 270         


Arg Glu Asp Val Gln Ala Leu Asn Ile Ser Val Pro Tyr Gly Pro Ile 
        275                 280                 285             


Pro Val Asp Phe Gln Arg Lys Ile Arg Gln Ser Tyr Phe Ala Ser Val 
    290                 295                 300                 


Ser Tyr Leu Asp Thr Gln Val Gly Arg Leu Leu Ser Ala Leu Asp Asp 
305                 310                 315                 320 


Leu Gln Leu Ala Asn Ser Thr Ile Ile Ala Phe Thr Ser Asp His Gly 
                325                 330                 335     


Trp Ala Leu Gly Glu His Gly Glu Trp Ala Lys Tyr Ser Asn Phe Asp 
            340                 345                 350         


Val Ala Thr His Val Pro Leu Ile Phe Tyr Val Pro Gly Arg Thr Ala 
        355                 360                 365             


Ser Leu Pro Glu Ala Gly Glu Lys Leu Phe Pro Tyr Leu Asp Pro Phe 
    370                 375                 380                 


Asp Ser Ala Ser Gln Leu Met Glu Pro Gly Arg Gln Ser Met Asp Leu 
385                 390                 395                 400 


Val Glu Leu Val Ser Leu Phe Pro Thr Leu Ala Gly Leu Ala Gly Leu 
                405                 410                 415     


Gln Val Pro Pro Arg Cys Pro Val Pro Ser Phe His Val Glu Leu Cys 
            420                 425                 430         


Arg Glu Gly Lys Asn Leu Leu Lys His Phe Arg Phe Arg Asp Leu Glu 
        435                 440                 445             


Glu Asp Pro Tyr Leu Pro Gly Asn Pro Arg Glu Leu Ile Ala Tyr Ser 
    450                 455                 460                 


Gln Tyr Pro Arg Pro Ser Asp Ile Pro Gln Trp Asn Ser Asp Lys Pro 
465                 470                 475                 480 


Ser Leu Lys Asp Ile Lys Ile Met Gly Tyr Ser Ile Arg Thr Ile Asp 
                485                 490                 495     


Tyr Arg Tyr Thr Val Trp Val Gly Phe Asn Pro Asp Glu Phe Leu Ala 
            500                 505                 510         


Asn Phe Ser Asp Ile His Ala Gly Glu Leu Tyr Phe Val Asp Ser Asp 
        515                 520                 525             


Pro Leu Gln Asp His Asn Met Tyr Asn Asp Ser Gln Gly Gly Asp Leu 
    530                 535                 540                 


Phe Gln Leu Leu Met Pro 
545                 550 


<210>  7
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ApoE tandem repeat (ApoEII)

<400>  7

Leu Arg Lys Leu Arg Lys Arg Leu Leu Leu Arg Lys Leu Arg Lys Arg 
1               5                   10                  15      


Leu Leu 
        


<210>  8
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  8

Leu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Gly Gly Ser 
            20      


