                         SEQUENCE LISTING

<110>  Henkel AG & Co. KGaA
 
<120>  Lipasen mit erhhter Thermostabilitt

<130>  PT034095 PCT

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  366
<212>  PRT
<213>  Rhizopus oryzae

<400>  1

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Pro Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Leu Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Lys Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Asn Phe Ser Asp Tyr Lys Pro Val Lys Gly Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


Gln Glu Gln Leu Thr Ala His Pro Thr Tyr Lys Val Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Pro Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val His Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Pro Gln Ser Phe Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asn Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Leu 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Asp Ile Asn Glu Gly Ser Cys Leu 
        355                 360                 365     


<210>  2
<211>  366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant of Rhizopus oryzae lipase

<400>  2

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Leu Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Leu Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Lys Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Asn Phe Ser Asp Tyr Lys Pro Val Lys Gly Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


Gln Glu Gln Leu Thr Ala His Pro Thr Tyr Lys Val Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Ser Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val Asn Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Pro Gln Ser Phe Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asn Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Leu 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Asp Ile Asn Glu Gly Ser Cys Leu 
        355                 360                 365     


<210>  3
<211>  366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant of Rhizopus oryzae lipase

<400>  3

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Pro Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Leu Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Lys Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Asn Phe Ser Asp Tyr Lys Pro Val Lys Gly Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


Gln Glu Gln Leu Thr Ala His Pro Thr Tyr Lys Met Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Pro Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val His Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Pro Gln Ser Phe Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asn Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Leu 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Asp Ile Asn Glu Gly Ser Cys Leu 
        355                 360                 365     


<210>  4
<211>  366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant of Rhizopus oryzae lipase

<400>  4

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Pro Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Leu Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Lys Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Asn Phe Ser Asp Tyr Lys Pro Val Lys Gly Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


Gln Glu Gln Leu Thr Ala His Pro Thr Tyr Lys Val Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Pro Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val His Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Pro Gln Ser Tyr Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asn Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Leu 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Gly Ile Asn Glu Gly Ser Cys Leu 
        355                 360                 365     


<210>  5
<211>  366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant of Rhizopus oryzae lipase

<400>  5

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Pro Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Leu Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Glu Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Asn Phe Ser Asp Tyr Lys Pro Val Lys Gly Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


Gln Glu Gln Leu Thr Ala His Pro Thr Tyr Lys Val Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Pro Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val His Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Pro Gln Ser Phe Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asn Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Leu 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Asp Ile Asn Glu Gly Ser Cys Leu 
        355                 360                 365     


<210>  6
<211>  366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant of Rhizopus oryzae lipase

<400>  6

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Pro Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Leu Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Lys Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Asn Phe Ser Asp Tyr Lys Pro Val Lys Gly Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


Gln Glu Gln Leu Thr Ala His Pro Thr Tyr Asn Val Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Pro Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val His Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Pro Gln Ser Phe Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asn Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Leu 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Asp Ile Asn Glu Gly Ser Cys Leu 
        355                 360                 365     


<210>  7
<211>  366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant of Rhizopus oryzae lipase

<400>  7

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Gly Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Pro Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Leu Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Lys Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Asn Phe Ser Asp Tyr Lys Pro Val Lys Val Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


Gln Glu Gln Leu Thr Ala His Pro Thr Tyr Lys Val Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Pro Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val His Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Gln Gln Ser Phe Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asn Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Leu 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Asp Ile Asn Glu Gly Ser Cys Leu 
        355                 360                 365     


<210>  8
<211>  366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant of Rhizopus oryzae lipase

<400>  8

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Pro Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Leu Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Lys Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Asn Phe Ser Asp Tyr Lys Pro Val Lys Gly Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


Gln Glu Gln Leu Thr Ala His Pro Thr Tyr Lys Val Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Pro Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val Asn Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Pro Gln Ser Phe Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asn Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Leu 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Asp Ile Asn Glu Gly Ser Cys Leu 
        355                 360                 365     


<210>  9
<211>  366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant of Rhizopus oryzae lipase

<400>  9

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Leu Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Pro Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Lys Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Asn Phe Ser Asp Tyr Lys Pro Val Lys Gly Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


Gln Glu Gln Leu Thr Ala His Pro Thr Tyr Lys Val Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Ser Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val Asn Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Pro Gln Ser Phe Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asn Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Leu 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Asp Ile Asn Glu Gly Ser Cys Leu 
        355                 360                 365     


<210>  10
<211>  366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant of Rhizopus oryzae lipase

<400>  10

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Leu Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Leu Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Lys Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Asn Phe Ser Asp Tyr Lys Pro Val Lys Gly Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


Gln Glu Gln Leu Thr Ala His Pro Thr Tyr Lys Val Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Ser Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val Asn Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Pro Gln Ser Phe Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asn Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Leu 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Asp Ile Asn Glu Gly Phe Cys Leu 
        355                 360                 365     


<210>  11
<211>  366
<212>  PRT
<213>  Artificial

<220>
<223>  Mutant of Rhizopus oryzae lipase

<400>  11

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Leu Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Leu Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Lys Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Asn Phe Ser Asp Tyr Lys Pro Val Lys Gly Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


His Glu Gln Leu Thr Ala His Pro Thr Tyr Lys Val Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Ser Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val Asn Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Pro Gln Ser Phe Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asn Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Leu 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Asp Ile Asn Glu Gly Ser Cys Leu 
        355                 360                 365     


<210>  12
<211>  366
<212>  PRT
<213>  Artificial

<220>
<223>  Mutant of Rhizopus oryzae lipase

<400>  12

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Leu Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Leu Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Lys Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Asn Phe Ser Asp Tyr Lys Pro Val Lys Gly Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


Gln Glu Gln Leu Thr Ala His Pro Thr Tyr Lys Val Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Ser Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val Asn Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Ser Gln Ser Phe Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asn Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Leu 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Asp Ile Asn Glu Gly Ser Cys Leu 
        355                 360                 365     


<210>  13
<211>  366
<212>  PRT
<213>  Artificial

<220>
<223>  Mutant of Rhizopus oryzae lipase

<400>  13

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Leu Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Leu Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Lys Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Asn Phe Ser Asp Tyr Lys Pro Val Lys Gly Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


Gln Glu Gln Leu Thr Ala His Pro Thr Tyr Lys Val Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Ser Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val Asn Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Pro Gln Ser Phe Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asp Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Leu 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Asp Ile Asn Glu Gly Ser Cys Leu 
        355                 360                 365     


<210>  14
<211>  366
<212>  PRT
<213>  Artificial

<220>
<223>  Mutant of Rhizopus oryzae lipase

<400>  14

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Pro Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Pro Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Lys Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Asn Phe Ser Asp Tyr Lys Pro Val Lys Gly Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


Gln Glu Gln Leu Thr Ala His Pro Thr Tyr Lys Val Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Pro Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val Asn Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Pro Gln Ser Phe Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asn Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Leu 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Asp Ile Asn Glu Gly Ser Cys Leu 
        355                 360                 365     


<210>  15
<211>  366
<212>  PRT
<213>  Artificial

<220>
<223>  Mutant of Rhizopus oryzae lipase

<400>  15

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Pro Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Leu Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Lys Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Asn Phe Ser Asp Tyr Lys Pro Val Lys Gly Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


Gln Glu Gln Leu Thr Ala His Pro Thr Tyr Lys Val Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Pro Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val Asn Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Pro Gln Ser Phe Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asn Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Leu 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Asp Ile Asn Glu Gly Phe Cys Leu 
        355                 360                 365     


<210>  16
<211>  366
<212>  PRT
<213>  Artificial

<220>
<223>  Mutant of Rhizopus oryzae lipase

<400>  16

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Pro Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Leu Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Lys Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Asn Phe Ser Asp Tyr Lys Pro Val Lys Gly Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


His Glu Gln Leu Thr Ala His Pro Thr Tyr Lys Val Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Pro Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val Asn Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Pro Gln Ser Phe Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asn Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Leu 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Asp Ile Asn Glu Gly Ser Cys Leu 
        355                 360                 365     


<210>  17
<211>  366
<212>  PRT
<213>  Artificial

<220>
<223>  Mutant of Rhizopus oryzae lipase

<400>  17

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Pro Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Leu Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Lys Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Asn Phe Ser Asp Tyr Lys Pro Val Lys Gly Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


Gln Glu Gln Leu Thr Ala His Pro Thr Tyr Lys Val Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Pro Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val Asn Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Ser Gln Ser Phe Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asn Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Leu 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Asp Ile Asn Glu Gly Ser Cys Leu 
        355                 360                 365     


<210>  18
<211>  366
<212>  PRT
<213>  Artificial

<220>
<223>  Mutant of Rhizopus oryzae lipase

<400>  18

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Pro Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Leu Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Lys Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Asn Phe Ser Asp Tyr Lys Pro Val Lys Gly Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


Gln Glu Gln Leu Thr Ala His Pro Thr Tyr Lys Val Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Pro Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val Asn Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Pro Gln Ser Phe Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asp Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Leu 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Asp Ile Asn Glu Gly Ser Cys Leu 
        355                 360                 365     


<210>  19
<211>  366
<212>  PRT
<213>  Artificial

<220>
<223>  Mutant of Rhizopus oryzae lipase

<400>  19

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Pro Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Leu Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Lys Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Glu Phe Ser Asp Tyr Lys Pro Val Lys Gly Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


Gln Glu Gln Leu Thr Ala His Pro Thr Tyr Lys Val Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Pro Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val Asn Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Pro Gln Ser Phe Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asn Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Leu 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Asp Ile Asn Glu Gly Phe Cys Leu 
        355                 360                 365     


<210>  20
<211>  366
<212>  PRT
<213>  Artificial

<220>
<223>  Mutant of Rhizopus oryzae lipase

<400>  20

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Pro Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Leu Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Lys Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Asn Phe Ser Asp Tyr Lys Pro Val Lys Gly Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


Gln Glu Gln Leu Thr Ala His Pro Thr Tyr Lys Val Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Pro Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val Asn Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Pro Gln Ser Phe Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asn Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Thr 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Asp Ile Asn Glu Gly Phe Cys Leu 
        355                 360                 365     


<210>  21
<211>  366
<212>  PRT
<213>  Artificial

<220>
<223>  Mutant of Rhizopus oryzae lipase

<400>  21

Val Pro Val Ser Gly Lys Ser Gly Ser Ser Asn Thr Ala Val Ser Ala 
1               5                   10                  15      


Ser Asp Asn Ala Ala Leu Pro Pro Leu Ile Ser Ser Arg Cys Ala Pro 
            20                  25                  30          


Pro Ser Asn Lys Gly Ser Lys Ser Asp Leu Gln Ala Glu Pro Tyr Asn 
        35                  40                  45              


Met Gln Lys Asn Thr Glu Trp Tyr Glu Ser His Gly Gly Asn Leu Thr 
    50                  55                  60                  


Ser Ile Gly Lys Arg Asp Asp Asn Leu Val Gly Gly Met Thr Leu Asp 
65                  70                  75                  80  


Leu Pro Ser Asp Ala Pro Pro Ile Ser Leu Ser Ser Ser Thr Asn Ser 
                85                  90                  95      


Ala Ser Asp Gly Gly Lys Val Val Ala Ala Thr Thr Ala Gln Ile Gln 
            100                 105                 110         


Glu Phe Thr Lys Tyr Ala Gly Ile Ala Ala Thr Ala Tyr Cys Arg Ser 
        115                 120                 125             


Val Val Pro Gly Asn Lys Trp Asp Cys Val Gln Cys Gln Lys Trp Val 
    130                 135                 140                 


Pro Asp Gly Lys Ile Ile Thr Thr Phe Thr Ser Leu Leu Ser Asp Thr 
145                 150                 155                 160 


Asn Gly Tyr Val Leu Arg Ser Asp Lys Gln Lys Thr Ile Tyr Leu Val 
                165                 170                 175     


Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile Thr Asp Ile Val Phe 
            180                 185                 190         


Glu Phe Ser Asp Tyr Lys Pro Val Lys Gly Ala Lys Val His Ala Gly 
        195                 200                 205             


Phe Leu Ser Ser Tyr Glu Gln Val Val Asn Asp Tyr Phe Pro Val Val 
    210                 215                 220                 


Gln Glu Gln Leu Thr Ala His Pro Thr Tyr Lys Val Ile Val Thr Gly 
225                 230                 235                 240 


His Ser Leu Gly Gly Ala Gln Ala Leu Leu Ala Gly Met Asp Leu Tyr 
                245                 250                 255     


Gln Arg Glu Pro Arg Leu Ser Pro Lys Asn Leu Ser Ile Phe Thr Val 
            260                 265                 270         


Gly Gly Pro Arg Val Gly Asn Pro Thr Phe Ala Tyr Tyr Val Glu Ser 
        275                 280                 285             


Thr Gly Ile Pro Phe Gln Arg Thr Val Asn Lys Arg Asp Ile Val Pro 
    290                 295                 300                 


His Val Pro Pro Gln Ser Phe Gly Phe Leu His Pro Gly Val Glu Ser 
305                 310                 315                 320 


Trp Ile Lys Ser Gly Thr Ser Asn Val Gln Ile Cys Thr Ser Glu Ile 
                325                 330                 335     


Glu Thr Lys Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Thr 
            340                 345                 350         


Asp His Leu Ser Tyr Phe Asp Ile Asn Glu Gly Phe Cys Leu 
        355                 360                 365     


