                         SEQUENCE LISTING

<110>  Manus Bio, Inc.
 
<120>  METABOLIC ENGINEERING FOR MICROBIAL PRODUCTION OF TERPENOID 
       PRODUCTS

<130>  MAN-008PC

<160>  8     

<170>  PatentIn version 3.5

<210>  1

<400>  1
000

<210>  2

<400>  2
000

<210>  3
<211>  323
<212>  PRT
<213>  Escherichia coli

<400>  3

Met Asn Leu Glu Lys Ile Asn Glu Leu Thr Ala Gln Asp Met Ala Gly 
1               5                   10                  15      


Val Asn Ala Ala Ile Leu Glu Gln Leu Asn Ser Asp Val Gln Leu Ile 
            20                  25                  30          


Asn Gln Leu Gly Tyr Tyr Ile Val Ser Gly Gly Gly Lys Arg Ile Arg 
        35                  40                  45              


Pro Met Ile Ala Val Leu Ala Ala Arg Ala Val Gly Tyr Glu Gly Asn 
    50                  55                  60                  


Ala His Val Thr Ile Ala Ala Leu Ile Glu Phe Ile His Thr Ala Thr 
65                  70                  75                  80  


Leu Leu His Asp Asp Val Val Asp Glu Ser Asp Met Arg Arg Gly Lys 
                85                  90                  95      


Ala Thr Ala Asn Ala Ala Phe Gly Asn Ala Ala Ser Val Leu Val Gly 
            100                 105                 110         


Asp Phe Ile Tyr Thr Arg Ala Phe Gln Met Met Thr Ser Leu Gly Ser 
        115                 120                 125             


Leu Lys Val Leu Glu Val Met Ser Glu Ala Val Asn Val Ile Ala Glu 
    130                 135                 140                 


Gly Glu Val Leu Gln Leu Met Asn Val Asn Asp Pro Asp Ile Thr Glu 
145                 150                 155                 160 


Glu Asn Tyr Met Arg Val Ile Tyr Ser Lys Thr Ala Arg Leu Phe Glu 
                165                 170                 175     


Ala Ala Ala Gln Cys Ser Gly Ile Leu Ala Gly Cys Thr Pro Glu Glu 
            180                 185                 190         


Glu Lys Gly Leu Gln Asp Tyr Gly Arg Tyr Leu Gly Thr Ala Phe Gln 
        195                 200                 205             


Leu Ile Asp Asp Leu Leu Asp Tyr Asn Ala Asp Gly Glu Gln Leu Gly 
    210                 215                 220                 


Lys Asn Val Gly Asp Asp Leu Asn Glu Gly Lys Pro Thr Leu Pro Leu 
225                 230                 235                 240 


Leu His Ala Met His His Gly Thr Pro Glu Gln Ala Gln Met Ile Arg 
                245                 250                 255     


Thr Ala Ile Glu Gln Gly Asn Gly Arg His Leu Leu Glu Pro Val Leu 
            260                 265                 270         


Glu Ala Met Asn Ala Cys Gly Ser Leu Glu Trp Thr Arg Gln Arg Ala 
        275                 280                 285             


Glu Glu Glu Ala Asp Lys Ala Ile Ala Ala Leu Gln Val Leu Pro Asp 
    290                 295                 300                 


Thr Pro Trp Arg Glu Ala Leu Ile Gly Leu Ala His Ile Ala Val Gln 
305                 310                 315                 320 


Arg Asp Arg 
            


<210>  4
<211>  398
<212>  PRT
<213>  Escherichia coli

<400>  4

Met Lys Gln Leu Thr Ile Leu Gly Ser Thr Gly Ser Ile Gly Cys Ser 
1               5                   10                  15      


Thr Leu Asp Val Val Arg His Asn Pro Glu His Phe Arg Val Val Ala 
            20                  25                  30          


Leu Val Ala Gly Lys Asn Val Thr Arg Met Val Glu Gln Cys Leu Glu 
        35                  40                  45              


Phe Ser Pro Arg Tyr Ala Val Met Asp Asp Glu Ala Ser Ala Lys Leu 
    50                  55                  60                  


Leu Lys Thr Met Leu Gln Gln Gln Gly Ser Arg Thr Glu Val Leu Ser 
65                  70                  75                  80  


Gly Gln Gln Ala Ala Cys Asp Met Ala Ala Leu Glu Asp Val Asp Gln 
                85                  90                  95      


Val Met Ala Ala Ile Val Gly Ala Ala Gly Leu Leu Pro Thr Leu Ala 
            100                 105                 110         


Ala Ile Arg Ala Gly Lys Thr Ile Leu Leu Ala Asn Lys Glu Ser Leu 
        115                 120                 125             


Val Thr Cys Gly Arg Leu Phe Met Asp Ala Val Lys Gln Ser Lys Ala 
    130                 135                 140                 


Gln Leu Leu Pro Val Asp Ser Glu His Asn Ala Ile Phe Gln Ser Leu 
145                 150                 155                 160 


Pro Gln Pro Ile Gln His Asn Leu Gly Tyr Ala Asp Leu Glu Gln Asn 
                165                 170                 175     


Gly Val Val Ser Ile Leu Leu Thr Gly Ser Gly Gly Pro Phe Arg Glu 
            180                 185                 190         


Thr Pro Leu Arg Asp Leu Ala Thr Met Thr Pro Asp Gln Ala Cys Arg 
        195                 200                 205             


His Pro Asn Trp Ser Met Gly Arg Lys Ile Ser Val Asp Ser Ala Thr 
    210                 215                 220                 


Met Met Asn Lys Gly Leu Glu Tyr Ile Glu Ala Arg Trp Leu Phe Asn 
225                 230                 235                 240 


Ala Ser Ala Ser Gln Met Glu Val Leu Ile His Pro Gln Ser Val Ile 
                245                 250                 255     


His Ser Met Val Arg Tyr Gln Asp Gly Ser Val Leu Ala Gln Leu Gly 
            260                 265                 270         


Glu Pro Asp Met Arg Thr Pro Ile Ala His Thr Met Ala Trp Pro Asn 
        275                 280                 285             


Arg Val Asn Ser Gly Val Lys Pro Leu Asp Phe Cys Lys Leu Ser Ala 
    290                 295                 300                 


Leu Thr Phe Ala Ala Pro Asp Tyr Asp Arg Tyr Pro Cys Leu Lys Leu 
305                 310                 315                 320 


Ala Met Glu Ala Phe Glu Gln Gly Gln Ala Ala Thr Thr Ala Leu Asn 
                325                 330                 335     


Ala Ala Asn Glu Ile Thr Val Ala Ala Phe Leu Ala Gln Gln Ile Arg 
            340                 345                 350         


Phe Thr Asp Ile Ala Ala Leu Asn Leu Ser Val Leu Glu Lys Met Asp 
        355                 360                 365             


Met Arg Glu Pro Gln Cys Val Asp Asp Val Leu Ser Val Asp Ala Asn 
    370                 375                 380                 


Ala Arg Glu Val Ala Arg Lys Glu Val Met Arg Leu Ala Ser 
385                 390                 395             


<210>  5
<211>  372
<212>  PRT
<213>  Escherichia coli

<400>  5

Met His Asn Gln Ala Pro Ile Gln Arg Arg Lys Ser Thr Arg Ile Tyr 
1               5                   10                  15      


Val Gly Asn Val Pro Ile Gly Asp Gly Ala Pro Ile Ala Val Gln Ser 
            20                  25                  30          


Met Thr Asn Thr Arg Thr Thr Asp Val Glu Ala Thr Val Asn Gln Ile 
        35                  40                  45              


Lys Ala Leu Glu Arg Val Gly Ala Asp Ile Val Arg Val Ser Val Pro 
    50                  55                  60                  


Thr Met Asp Ala Ala Glu Ala Phe Lys Leu Ile Lys Gln Gln Val Asn 
65                  70                  75                  80  


Val Pro Leu Val Ala Asp Ile His Phe Asp Tyr Arg Ile Ala Leu Lys 
                85                  90                  95      


Val Ala Glu Tyr Gly Val Asp Cys Leu Arg Ile Asn Pro Gly Asn Ile 
            100                 105                 110         


Gly Asn Glu Glu Arg Ile Arg Met Val Val Asp Cys Ala Arg Asp Lys 
        115                 120                 125             


Asn Ile Pro Ile Arg Ile Gly Val Asn Ala Gly Ser Leu Glu Lys Asp 
    130                 135                 140                 


Leu Gln Glu Lys Tyr Gly Glu Pro Thr Pro Gln Ala Leu Leu Glu Ser 
145                 150                 155                 160 


Ala Met Arg His Val Asp His Leu Asp Arg Leu Asn Phe Asp Gln Phe 
                165                 170                 175     


Lys Val Ser Val Lys Ala Ser Asp Val Phe Leu Ala Val Glu Ser Tyr 
            180                 185                 190         


Arg Leu Leu Ala Lys Gln Ile Asp Gln Pro Leu His Leu Gly Ile Thr 
        195                 200                 205             


Glu Ala Gly Gly Ala Arg Ser Gly Ala Val Lys Ser Ala Ile Gly Leu 
    210                 215                 220                 


Gly Leu Leu Leu Ser Glu Gly Ile Gly Asp Thr Leu Arg Val Ser Leu 
225                 230                 235                 240 


Ala Ala Asp Pro Val Glu Glu Ile Lys Val Gly Phe Asp Ile Leu Lys 
                245                 250                 255     


Ser Leu Arg Ile Arg Ser Arg Gly Ile Asn Phe Ile Ala Cys Pro Thr 
            260                 265                 270         


Cys Ser Arg Gln Glu Phe Asp Val Ile Gly Thr Val Asn Ala Leu Glu 
        275                 280                 285             


Gln Arg Leu Glu Asp Ile Ile Thr Pro Met Asp Val Ser Ile Ile Gly 
    290                 295                 300                 


Cys Val Val Asn Gly Pro Gly Glu Ala Leu Val Ser Thr Leu Gly Val 
305                 310                 315                 320 


Thr Gly Gly Asn Lys Lys Ser Gly Leu Tyr Glu Asp Gly Val Arg Lys 
                325                 330                 335     


Asp Arg Leu Asp Asn Asn Asp Met Ile Asp Gln Leu Glu Ala Arg Ile 
            340                 345                 350         


Arg Ala Lys Ala Ser Gln Leu Asp Glu Ala Arg Arg Ile Asp Val Gln 
        355                 360                 365             


Gln Val Glu Lys 
    370         


<210>  6
<211>  316
<212>  PRT
<213>  Escherichia coli

<400>  6

Met Gln Ile Leu Leu Ala Asn Pro Arg Gly Phe Cys Ala Gly Val Asp 
1               5                   10                  15      


Arg Ala Ile Ser Ile Val Glu Asn Ala Leu Ala Ile Tyr Gly Ala Pro 
            20                  25                  30          


Ile Tyr Val Arg His Glu Val Val His Asn Arg Tyr Val Val Asp Ser 
        35                  40                  45              


Leu Arg Glu Arg Gly Ala Ile Phe Ile Glu Gln Ile Ser Glu Val Pro 
    50                  55                  60                  


Asp Gly Ala Ile Leu Ile Phe Ser Ala His Gly Val Ser Gln Ala Val 
65                  70                  75                  80  


Arg Asn Glu Ala Lys Ser Arg Asp Leu Thr Val Phe Asp Ala Thr Cys 
                85                  90                  95      


Pro Leu Val Thr Lys Val His Met Glu Val Ala Arg Ala Ser Arg Arg 
            100                 105                 110         


Gly Glu Glu Ser Ile Leu Ile Gly His Ala Gly His Pro Glu Val Glu 
        115                 120                 125             


Gly Thr Met Gly Gln Tyr Ser Asn Pro Glu Gly Gly Met Tyr Leu Val 
    130                 135                 140                 


Glu Ser Pro Asp Asp Val Trp Lys Leu Thr Val Lys Asn Glu Glu Lys 
145                 150                 155                 160 


Leu Ser Phe Met Thr Gln Thr Thr Leu Ser Val Asp Asp Thr Ser Asp 
                165                 170                 175     


Val Ile Asp Ala Leu Arg Lys Arg Phe Pro Lys Ile Val Gly Pro Arg 
            180                 185                 190         


Lys Asp Asp Ile Cys Tyr Ala Thr Thr Asn Arg Gln Glu Ala Val Arg 
        195                 200                 205             


Ala Leu Ala Glu Gln Ala Glu Val Val Leu Val Val Gly Ser Lys Asn 
    210                 215                 220                 


Ser Ser Asn Ser Asn Arg Leu Ala Glu Leu Ala Gln Arg Met Gly Lys 
225                 230                 235                 240 


Arg Ala Phe Leu Ile Asp Asp Ala Lys Asp Ile Gln Glu Glu Trp Val 
                245                 250                 255     


Lys Glu Val Lys Cys Val Gly Val Thr Ala Gly Ala Ser Ala Pro Asp 
            260                 265                 270         


Ile Leu Val Gln Asn Val Val Ala Arg Leu Gln Gln Leu Gly Gly Gly 
        275                 280                 285             


Glu Ala Ile Pro Leu Glu Gly Arg Glu Glu Asn Ile Val Phe Glu Val 
    290                 295                 300                 


Pro Lys Glu Leu Arg Val Asp Ile Arg Glu Val Asp 
305                 310                 315     


<210>  7
<211>  438
<212>  PRT
<213>  Brucella abortus

<400>  7

Met Thr Thr Asn Val Ala Leu Val Gly Leu Ala Arg Asp Leu Ala Ala 
1               5                   10                  15      


Arg Ala Glu Thr Gly Lys Pro Ile Arg Ile Gly Leu Ile Gly Ala Gly 
            20                  25                  30          


Glu Met Gly Thr Asp Ile Val Thr Gln Val Ala Arg Met Gln Gly Ile 
        35                  40                  45              


Glu Val Gly Ala Leu Ser Ala Arg Arg Leu Pro Asn Thr Phe Lys Ala 
    50                  55                  60                  


Ile Arg Thr Ala Tyr Gly Asp Glu Glu Asn Ala Arg Glu Ala Thr Thr 
65                  70                  75                  80  


Glu Ser Ala Met Thr Arg Ala Ile Glu Ala Gly Lys Ile Ala Val Thr 
                85                  90                  95      


Asp Asp Asn Asp Leu Ile Leu Ser Asn Pro Leu Ile Asp Val Ile Ile 
            100                 105                 110         


Asp Ala Thr Gly Ile Pro Glu Val Gly Ala Glu Thr Gly Ile Ala Ala 
        115                 120                 125             


Ile Arg Asn Gly Lys His Leu Val Met Met Asn Val Glu Ala Asp Val 
    130                 135                 140                 


Thr Ile Gly Pro Tyr Leu Lys Ala Gln Ala Asp Lys Gln Gly Val Ile 
145                 150                 155                 160 


Tyr Ser Leu Gly Ala Gly Asp Glu Pro Ser Ser Cys Met Glu Leu Ile 
                165                 170                 175     


Glu Phe Val Ser Ala Leu Gly Tyr Glu Val Val Ser Ala Gly Lys Gly 
            180                 185                 190         


Lys Asn Asn Pro Leu Asn Phe Asp Ala Thr Pro Asp Asp Tyr Arg Gln 
        195                 200                 205             


Glu Ala Asp Arg Arg Asn Met Asn Val Arg Leu Leu Val Glu Phe Ile 
    210                 215                 220                 


Asp Gly Ser Lys Thr Met Val Glu Met Ala Ala Ile Ala Asn Ala Thr 
225                 230                 235                 240 


Gly Leu Val Pro Asp Ile Ala Gly Met His Gly Pro Arg Ala Ser Ile 
                245                 250                 255     


Asp Gln Leu Ser His Thr Leu Ile Pro Gln Ala Glu Gly Gly Val Leu 
            260                 265                 270         


Ser Lys Ser Gly Val Val Asp Tyr Ser Ile Gly Lys Gly Val Ser Pro 
        275                 280                 285             


Gly Val Phe Val Val Ala Lys Met Asp His Pro Arg Leu Asn Glu Arg 
    290                 295                 300                 


Leu Glu Asp Leu Lys Ile Gly Lys Gly Pro Tyr Phe Thr Phe His Arg 
305                 310                 315                 320 


Pro Tyr His Leu Thr Ser Leu Glu Val Pro Leu Thr Val Ala Arg Val 
                325                 330                 335     


Val Leu His Gly Lys Thr Asp Met Val Pro Leu Pro Lys Pro Val Ala 
            340                 345                 350         


Glu Val Cys Ala Val Ala Lys Lys Asp Met Gln Pro Gly Glu His Leu 
        355                 360                 365             


Asp Ala Ile Gly Gln Tyr Cys Tyr Arg Ser Trp Ile Met Thr Val Pro 
    370                 375                 380                 


Glu Ala Arg Ala Ala Lys Ala Ile Pro Cys Gly Leu Leu Gln Asn Gly 
385                 390                 395                 400 


Thr Val Ile Ala Pro Ile Lys Lys Gly Glu Leu Ile Thr Tyr Ala Asn 
                405                 410                 415     


Ala Ala Pro Gln Pro Gly Ser Arg Ile Ala Glu Leu Arg Ala Leu Gln 
            420                 425                 430         


Asp Ala Met Leu Gly Gln 
        435             


<210>  8
<211>  283
<212>  PRT
<213>  Escherichia coli

<400>  8

Met Arg Thr Gln Trp Pro Ser Pro Ala Lys Leu Asn Leu Phe Leu Tyr 
1               5                   10                  15      


Ile Thr Gly Gln Arg Ala Asp Gly Tyr His Thr Leu Gln Thr Leu Phe 
            20                  25                  30          


Gln Phe Leu Asp Tyr Gly Asp Thr Ile Ser Ile Glu Leu Arg Asp Asp 
        35                  40                  45              


Gly Asp Ile Arg Leu Leu Thr Pro Val Glu Gly Val Glu His Glu Asp 
    50                  55                  60                  


Asn Leu Ile Val Arg Ala Ala Arg Leu Leu Met Lys Thr Ala Ala Asp 
65                  70                  75                  80  


Ser Gly Arg Leu Pro Thr Gly Ser Gly Ala Asn Ile Ser Ile Asp Lys 
                85                  90                  95      


Arg Leu Pro Met Gly Gly Gly Leu Gly Gly Gly Ser Ser Asn Ala Ala 
            100                 105                 110         


Thr Val Leu Val Ala Leu Asn His Leu Trp Gln Cys Gly Leu Ser Met 
        115                 120                 125             


Asp Glu Leu Ala Glu Met Gly Leu Thr Leu Gly Ala Asp Val Pro Val 
    130                 135                 140                 


Phe Val Arg Gly His Ala Ala Phe Ala Glu Gly Val Gly Glu Ile Leu 
145                 150                 155                 160 


Thr Pro Val Asp Pro Pro Glu Lys Trp Tyr Leu Val Ala His Pro Gly 
                165                 170                 175     


Val Ser Ile Pro Thr Pro Val Ile Phe Lys Asp Pro Glu Leu Pro Arg 
            180                 185                 190         


Asn Thr Pro Lys Arg Ser Ile Glu Thr Leu Leu Lys Cys Glu Phe Ser 
        195                 200                 205             


Asn Asp Cys Glu Val Ile Ala Arg Lys Arg Phe Arg Glu Val Asp Ala 
    210                 215                 220                 


Val Leu Ser Trp Leu Leu Glu Tyr Ala Pro Ser Arg Leu Thr Gly Thr 
225                 230                 235                 240 


Gly Ala Cys Val Phe Ala Glu Phe Asp Thr Glu Ser Glu Ala Arg Gln 
                245                 250                 255     


Val Leu Glu Gln Ala Pro Glu Trp Leu Asn Gly Phe Val Ala Lys Gly 
            260                 265                 270         


Ala Asn Leu Ser Pro Leu His Arg Ala Met Leu 
        275                 280             


