                               SEQUENCE LISTING

<110> ABBVIE INC.
 
<120> ENZYMATIC PROCESSES FOR THE PREPARATION OF 
      (PLUS/MINUS)-2-(DIFLUOROMETHYL)-1-(ALKOXYCARBONYL)-
      CYCLOPROPANECARBOXYLIC ACID AND (PLUS/MINUS)-2-(VINYL)-
      1-(ALKOXYCARBONYL)-CYCLOPROPANECARBOXYLIC ACID

<130> AVR-05225

<140> PCT/US2018/016362
<141> 2018-02-01

<150> 62/453,246
<151> 2017-02-01

<160> 4     

<170> PatentIn version 3.5

<210> 1
<211> 750
<212> DNA
<213> Bacillus stearothermophilus

<400> 1
atggcgaaga ttgtcccgcc gaagccgttt ttctttgaag ccggtgaacg tgctgtcctg       60

ctgctgcatg gttttaccgg taactccgct gatgtgcgta tgctgggccg ctttctggaa      120

tccaaaggtt atacctgcca tgcgccgatt tacaagggcc acggtgtccc gccggaagaa      180

ctggtgcata cgggtccgga tgactggtgg caggatgtta tgaacggcta cgaatttctg      240

aagaacaagg gttacgaaaa gatcgcagtg gcaggtctga gcctgggcgg tgttttcagc      300

ctgaaactgg gctataccgt tccgattgaa ggtatcgtca cgatgtgtgc accgatgtat      360

attaaaagcg aagaaaccat gtacgaaggt gtgctggaat atgctcgtga atacaaaaag      420

cgcgaaggca aatctgaaga acagatcgaa caagaaatgg aaaagttcaa gcagaccccg      480

atgaaaacgc tgaaggcact gcaagaactg attgctgatg ttcgtgacca tctggatctg      540

atctatgcac cgaccttcgt ggttcaggca cgtcacgacg aaatgattaa cccggattca      600

gcgaacatca tctacaacga aatcgaatcg ccggtcaaac agatcaagtg gtacgaacaa      660

agcggtcacg tgattacgct ggaccaggaa aaagatcaac tgcatgaaga catctacgcc      720

ttcctggaaa gtctggattg gtgactcgag                                       750


<210> 2
<211> 247
<212> PRT
<213> Bacillus stearothermophilus

<400> 2
Met Ala Lys Ile Val Pro Pro Lys Pro Phe Phe Phe Glu Ala Gly Glu 
1               5                   10                  15      


Arg Ala Val Leu Leu Leu His Gly Phe Thr Gly Asn Ser Ala Asp Val 
            20                  25                  30          


Arg Met Leu Gly Arg Phe Leu Glu Ser Lys Gly Tyr Thr Cys His Ala 
        35                  40                  45              


Pro Ile Tyr Lys Gly His Gly Val Pro Pro Glu Glu Leu Val His Thr 
    50                  55                  60                  


Gly Pro Asp Asp Trp Trp Gln Asp Val Met Asn Gly Tyr Glu Phe Leu 
65                  70                  75                  80  


Lys Asn Lys Gly Tyr Glu Lys Ile Ala Val Ala Gly Leu Ser Leu Gly 
                85                  90                  95      


Gly Val Phe Ser Leu Lys Leu Gly Tyr Thr Val Pro Ile Glu Gly Ile 
            100                 105                 110         


Val Thr Met Cys Ala Pro Met Tyr Ile Lys Ser Glu Glu Thr Met Tyr 
        115                 120                 125             


Glu Gly Val Leu Glu Tyr Ala Arg Glu Tyr Lys Lys Arg Glu Gly Lys 
    130                 135                 140                 


Ser Glu Glu Gln Ile Glu Gln Glu Met Glu Lys Phe Lys Gln Thr Pro 
145                 150                 155                 160 


Met Lys Thr Leu Lys Ala Leu Gln Glu Leu Ile Ala Asp Val Arg Asp 
                165                 170                 175     


His Leu Asp Leu Ile Tyr Ala Pro Thr Phe Val Val Gln Ala Arg His 
            180                 185                 190         


Asp Glu Met Ile Asn Pro Asp Ser Ala Asn Ile Ile Tyr Asn Glu Ile 
        195                 200                 205             


Glu Ser Pro Val Lys Gln Ile Lys Trp Tyr Glu Gln Ser Gly His Val 
    210                 215                 220                 


Ile Thr Leu Asp Gln Glu Lys Asp Gln Leu His Glu Asp Ile Tyr Ala 
225                 230                 235                 240 


Phe Leu Glu Ser Leu Asp Trp 
                245         


<210> 3
<211> 744
<212> DNA
<213> Bacillus stearothermophilus

<400> 3
atgggcaaaa tcgtcccgcc gaaaccgttc ttctttgaag ctggtgaacg tgctgtgctg       60

ctgctgcatg gctttcatgg caatagcgcc gacgttcgta tgctgggccg ctttctggaa      120

agcaaaggtt atacctgcca tgcaccgatt tacaaaggcc acggtgtccc gccggaagaa      180

ctggtgcata cgggtccgga tgactggtgg caggatgtca tgaacggcta cgaatttctg      240

aaaaacaaag gttacgaaaa aatcgcagtg gcaggtcata gcctgggtgg tgttttctct      300

ctgaaactgg gctataccgt tccgattgaa ggtatcgtca cgatgtgtgc accgatgtac      360

atcttatcag aagaaaccat gtacgaaggt gtgctggaat atgctcgtga atacaaaaaa      420

cgcgaaggca aatcggaaga acagatcgaa caagaaatgg aaaaattcaa acagaccccg      480

atgaaaacgc tgaaagcact gcaagaactg attgctgatg ttcgtgacca tctggatctg      540

atctatgcac cgaccttcgt ggttcaggca cgtcacgacg aaatgattaa cccggatagt      600

gccaacatca tctacaacga aatcgaatcc ccggtcaaac agatcaaatg gtacgaacaa      660

agtggccacg tgatcacgct ggatcaggaa aaagaccaac tgcacgaaga catttacgca      720

tttctggaat cgctggactg gtaa                                             744


<210> 4
<211> 247
<212> PRT
<213> Bacillus stearothermophilus

<400> 4
Met Gly Lys Ile Val Pro Pro Lys Pro Phe Phe Phe Glu Ala Gly Glu 
1               5                   10                  15      


Arg Ala Val Leu Leu Leu His Gly Phe His Gly Asn Ser Ala Asp Val 
            20                  25                  30          


Arg Met Leu Gly Arg Phe Leu Glu Ser Lys Gly Tyr Thr Cys His Ala 
        35                  40                  45              


Pro Ile Tyr Lys Gly His Gly Val Pro Pro Glu Glu Leu Val His Thr 
    50                  55                  60                  


Gly Pro Asp Asp Trp Trp Gln Asp Val Met Asn Gly Tyr Glu Phe Leu 
65                  70                  75                  80  


Lys Asn Lys Gly Tyr Glu Lys Ile Ala Val Ala Gly His Ser Leu Gly 
                85                  90                  95      


Gly Val Phe Ser Leu Lys Leu Gly Tyr Thr Val Pro Ile Glu Gly Ile 
            100                 105                 110         


Val Thr Met Cys Ala Pro Met Tyr Ile Leu Ser Glu Glu Thr Met Tyr 
        115                 120                 125             


Glu Gly Val Leu Glu Tyr Ala Arg Glu Tyr Lys Lys Arg Glu Gly Lys 
    130                 135                 140                 


Ser Glu Glu Gln Ile Glu Gln Glu Met Glu Lys Phe Lys Gln Thr Pro 
145                 150                 155                 160 


Met Lys Thr Leu Lys Ala Leu Gln Glu Leu Ile Ala Asp Val Arg Asp 
                165                 170                 175     


His Leu Asp Leu Ile Tyr Ala Pro Thr Phe Val Val Gln Ala Arg His 
            180                 185                 190         


Asp Glu Met Ile Asn Pro Asp Ser Ala Asn Ile Ile Tyr Asn Glu Ile 
        195                 200                 205             


Glu Ser Pro Val Lys Gln Ile Lys Trp Tyr Glu Gln Ser Gly His Val 
    210                 215                 220                 


Ile Thr Leu Asp Gln Glu Lys Asp Gln Leu His Glu Asp Ile Tyr Ala 
225                 230                 235                 240 


Phe Leu Glu Ser Leu Asp Trp 
                245         


