                         SEQUENCE LISTING

<110>  The Research Foundation for The State University of New 
       York
       New York University
 
<120>  MUTANT ISLET AMYLOID POLYPEPTIDES WITH IMPROVED SOLUBILITY AND 
       METHODS FOR USING THE SAME

<130>  34577

<150>  62/453,068
<151>  2017-02-01

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  37
<212>  PRT
<213>  Homo sapiens

<400>  1

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Asn Phe Gly Ala Ile Leu Ser Ser Thr Asn Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  2
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant islet amyloid polypeptide with amidated c-terminal and 
       proline at positions 25, 28 and 29

<400>  2

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Asn Phe Gly Pro Ile Leu Pro Pro Thr Asn Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  3
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant-human islet amyloid polypeptide, amino acid substitutions 
       at positions 21 and 35

<400>  3

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Lys Asn Phe Gly Ala Ile Leu Ser Ser Thr Asn Val 
            20                  25                  30          


Gly Ser Lys Thr Tyr 
        35          


<210>  4
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant-human islet amyloid polypeptide, amino acid substitutions 
       at positions 21, 24, 26 and 35

<400>  4

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Lys Asn Phe Pro Ala Pro Leu Ser Ser Thr Asn Val 
            20                  25                  30          


Gly Ser Lys Thr Tyr 
        35          


<210>  5
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant-human islet amyloid polypeptide, amino acid substitutions 
       at positions 22 and 31

<400>  5

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Lys Phe Gly Ala Ile Leu Ser Ser Thr Lys Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  6
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant-human islet amyloid polypeptide, amino acid substitutions 
       at positions 22, 24, 26 and 31

<400>  6

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Lys Phe Pro Ala Pro Leu Ser Ser Thr Lys Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  7
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant-human islet amyloid polypeptide, with amidated c-terminal 
       and amino acid substitutions at positions 21 and 35


<220>
<221>  MOD_RES
<222>  (37)..(37)
<223>  AMIDATION

<400>  7

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Lys Asn Phe Gly Ala Ile Leu Ser Ser Thr Asn Val 
            20                  25                  30          


Gly Ser Lys Thr Tyr 
        35          


<210>  8
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant-human islet amyloid polypeptide, with amidated c-terminal 
       and amino acid substitutions at positions 21, 24, 26 and 35


<220>
<221>  MOD_RES
<222>  (37)..(37)
<223>  AMIDATION

<400>  8

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Lys Asn Phe Pro Ala Pro Leu Ser Ser Thr Asn Val 
            20                  25                  30          


Gly Ser Lys Thr Tyr 
        35          


<210>  9
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant-human islet amyloid polypeptide, with amidated c-terminal 
       and amino acid substitutions at positions 22 and 31


<220>
<221>  MOD_RES
<222>  (37)..(37)
<223>  AMIDATION

<400>  9

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Lys Phe Gly Ala Ile Leu Ser Ser Thr Lys Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  10
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant-human islet amyloid polypeptide, with amidated c-terminal 
       and amino acid substitutions at positions 22, 24, 26 and 31


<220>
<221>  MOD_RES
<222>  (37)..(37)
<223>  AMIDATION

<400>  10

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Lys Phe Pro Ala Pro Leu Ser Ser Thr Lys Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  11
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant-human islet amyloid polypeptide, amino acid substitutions 
       at positions 21, 24 and 35

<400>  11

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Lys Asn Phe Pro Ala Ile Leu Ser Ser Thr Asn Val 
            20                  25                  30          


Gly Ser Lys Thr Tyr 
        35          


<210>  12
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant-human islet amyloid polypeptide, amino acid substitutions 
       at positions 21, 26 and 35

<400>  12

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Lys Asn Phe Gly Ala Pro Leu Ser Ser Thr Asn Val 
            20                  25                  30          


Gly Ser Lys Thr Tyr 
        35          


<210>  13
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant-human islet amyloid polypeptide, amino acid substitutions 
       at positions 22, 24 and 31

<400>  13

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Lys Phe Pro Ala Ile Leu Ser Ser Thr Lys Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  14
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant-human islet amyloid polypeptide, amino acid substitutions 
       at positions 22, 26 and 31

<400>  14

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Lys Phe Gly Ala Pro Leu Ser Ser Thr Lys Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  15
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant-human islet amyloid polypeptide, amino acid substitutions 
       at positions 21, 28 and 35

<400>  15

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Lys Asn Phe Gly Ala Ile Leu Pro Ser Thr Asn Val 
            20                  25                  30          


Gly Ser Lys Thr Tyr 
        35          


<210>  16
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant-human islet amyloid polypeptide, amino acid substitutions 
       at positions 22, 28 and 31

<400>  16

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Lys Phe Gly Ala Ile Leu Pro Ser Thr Lys Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  17
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant-human islet amyloid polypeptide with amidated c-terminal, 
       amino acid substitutions at positions 21, 24 and 35


<220>
<221>  MOD_RES
<222>  (37)..(37)
<223>  amidation

<400>  17

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Lys Asn Phe Pro Ala Ile Leu Ser Ser Thr Asn Val 
            20                  25                  30          


Gly Ser Lys Thr Tyr 
        35          


<210>  18
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant-human islet amyloid polypeptide with amidated C-terminus, 
       amino acid substitutions at positions 21, 26 and 35


<220>
<221>  MOD_RES
<222>  (37)..(37)
<223>  amidation

<400>  18

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Lys Asn Phe Gly Ala Pro Leu Ser Ser Thr Asn Val 
            20                  25                  30          


Gly Ser Lys Thr Tyr 
        35          


<210>  19
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant-human islet amyloid polypeptide with an amidated 
       c-terminus, amino acid substitutions at positions 21, 28 and 35


<220>
<221>  MOD_RES
<222>  (37)..(37)
<223>  amidation

<400>  19

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Lys Asn Phe Gly Ala Ile Leu Pro Ser Thr Asn Val 
            20                  25                  30          


Gly Ser Lys Thr Tyr 
        35          


<210>  20
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant-human islet amyloid polypeptide with an amidated 
       c-terminus, amino acid substitutions at positions 22, 24 and 31


<220>
<221>  MOD_RES
<222>  (37)..(37)
<223>  amidation

<400>  20

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Lys Phe Pro Ala Ile Leu Ser Ser Thr Lys Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  21
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant-human islet amyloid polypeptide with an amidated 
       c-terminus, amino acid substitutions at positions 22, 26 and 31


<220>
<221>  MOD_RES
<222>  (37)..(37)
<223>  amidation

<400>  21

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Lys Phe Gly Ala Pro Leu Ser Ser Thr Lys Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  22
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant-human islet amyloid polypeptide with an amidated 
       c-terminus, amino acid substitutions at positions 22, 28 and 31


<220>
<221>  MOD_RES
<222>  (37)..(37)
<223>  amidation

<400>  22

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Lys Phe Gly Ala Ile Leu Pro Ser Thr Lys Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


