                         SEQUENCE LISTING

<110>  Regents of the University of Minnesota
       Illinois State University
 
<120>  PLANTS HAVING INCREASED OIL QUALITY

<130>  09531-0389WO1

<150>  US 62/451,467
<151>  2017-01-27

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  1521
<212>  DNA
<213>  Thlaspi arvense

<400>  1
atgacgtccg ttaacgttaa gctcctttac cattacgtca tcaccaactt tttcaacctt       60

tgcttcttcc cgttagcggc gatcgttgcc ggaaaagcct ctcggcttac cacaaacgat      120

cttcaccact tctactattc ctatctccaa cacaacctaa taaccatatc tctactcttt      180

gccttcaccg ttttcggttt ggctctctac atcgtaaccc ggcccaaacc ggtttacctc      240

gttgaccatt cctgctacct tccaccatcg catcttagaa gcagtatctc taaggtcatg      300

gatatcttct atcaagtaag attagccgat cctttacgga acgcggcaag cgatgattcg      360

tcctggcttg atttcttgag gaagattcag gagcggtctg gtctaggcga tgaaacccac      420

ggccccgagg gactgcttca ggtccctcca cggaagactt ttgccgcggc gcgtgaagaa      480

acagagcaag tgatcatcgg tgcgctcgaa aaactattcg agaacaccaa agttaaccct      540

aaagagattg gtatacttgt ggtgaactca agcatgttta atccgactcc ttcgctctcg      600

gcgatggttg ttaatacttt caagctccga agcaacatca gaagctttaa tcttggagga      660

atgggttgta gtgccggcgt tatagccatt gatctggcta aggacttgtt gcatgtccat      720

aaaaacactt atgctcttgt ggtgagcaca gagaacatca cttacaacat ttatgctggt      780

gataacagat ccatgatggt ttcgaattgc ttgttccgtg ttggtggggc cgcgattttg      840

ctctccaaca agccgaggga ccggagacgg tccaagtacc agctacttca cacggttcgg      900

acgcataccg gagctgacga caagtctttc cgatgtgtgc aacaagaaga cgacgagagc      960

ggtaaaaccg gggtgtgttt gtccaaggac ataaccggtg ttgccgggag aactgttcag     1020

aaaaacataa caacattggg tccgttggtt cttcctttta gcgagaaatt tctttttttc     1080

gttaccttca tcgccaagaa actctttaaa gacaagatca aacattacta cgtcccggat     1140

ttcaagcttg ctatcgacca tttttgtatt catgccggag gcagagccgt gatcgatgtg     1200

ctacagaaga acttaggtct attgccgatc gatgtggagg catctaggtc aacgttacat     1260

agatttggga acacttcgtc tagctcaatt tggtatgaat tggcgtacat agaggcaaaa     1320

ggaaggatga agagagggaa caaagtttgg cagattgctt tagggtcagg gtttaagtgt     1380

aatagtgcgg tttgggtggc tctacgcaat gtcaaggctt cgacaaatag tccttgggaa     1440

cattgcattg atagatatcc agatgcaatt gattctgatt cgggtaagtc agagactcgt     1500

gtccaaaacg gtcggtccta a                                               1521


<210>  2
<211>  506
<212>  PRT
<213>  Thlaspi arvense

<400>  2

Met Thr Ser Val Asn Val Lys Leu Leu Tyr His Tyr Val Ile Thr Asn 
1               5                   10                  15      


Phe Phe Asn Leu Cys Phe Phe Pro Leu Ala Ala Ile Val Ala Gly Lys 
            20                  25                  30          


Ala Ser Arg Leu Thr Thr Asn Asp Leu His His Phe Tyr Tyr Ser Tyr 
        35                  40                  45              


Leu Gln His Asn Leu Ile Thr Ile Ser Leu Leu Phe Ala Phe Thr Val 
    50                  55                  60                  


Phe Gly Leu Ala Leu Tyr Ile Val Thr Arg Pro Lys Pro Val Tyr Leu 
65                  70                  75                  80  


Val Asp His Ser Cys Tyr Leu Pro Pro Ser His Leu Arg Ser Ser Ile 
                85                  90                  95      


Ser Lys Val Met Asp Ile Phe Tyr Gln Val Arg Leu Ala Asp Pro Leu 
            100                 105                 110         


Arg Asn Ala Ala Ser Asp Asp Ser Ser Trp Leu Asp Phe Leu Arg Lys 
        115                 120                 125             


Ile Gln Glu Arg Ser Gly Leu Gly Asp Glu Thr His Gly Pro Glu Gly 
    130                 135                 140                 


Leu Leu Gln Val Pro Pro Arg Lys Thr Phe Ala Ala Ala Arg Glu Glu 
145                 150                 155                 160 


Thr Glu Gln Val Ile Ile Gly Ala Leu Glu Lys Leu Phe Glu Asn Thr 
                165                 170                 175     


Lys Val Asn Pro Lys Glu Ile Gly Ile Leu Val Val Asn Ser Ser Met 
            180                 185                 190         


Phe Asn Pro Thr Pro Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys 
        195                 200                 205             


Leu Arg Ser Asn Ile Arg Ser Phe Asn Leu Gly Gly Met Gly Cys Ser 
    210                 215                 220                 


Ala Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu His Val His 
225                 230                 235                 240 


Lys Asn Thr Tyr Ala Leu Val Val Ser Thr Glu Asn Ile Thr Tyr Asn 
                245                 250                 255     


Ile Tyr Ala Gly Asp Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe 
            260                 265                 270         


Arg Val Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro Arg Asp Arg 
        275                 280                 285             


Arg Arg Ser Lys Tyr Gln Leu Leu His Thr Val Arg Thr His Thr Gly 
    290                 295                 300                 


Ala Asp Asp Lys Ser Phe Arg Cys Val Gln Gln Glu Asp Asp Glu Ser 
305                 310                 315                 320 


Gly Lys Thr Gly Val Cys Leu Ser Lys Asp Ile Thr Gly Val Ala Gly 
                325                 330                 335     


Arg Thr Val Gln Lys Asn Ile Thr Thr Leu Gly Pro Leu Val Leu Pro 
            340                 345                 350         


Phe Ser Glu Lys Phe Leu Phe Phe Val Thr Phe Ile Ala Lys Lys Leu 
        355                 360                 365             


Phe Lys Asp Lys Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 
    370                 375                 380                 


Ile Asp His Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp Val 
385                 390                 395                 400 


Leu Gln Lys Asn Leu Gly Leu Leu Pro Ile Asp Val Glu Ala Ser Arg 
                405                 410                 415     


Ser Thr Leu His Arg Phe Gly Asn Thr Ser Ser Ser Ser Ile Trp Tyr 
            420                 425                 430         


Glu Leu Ala Tyr Ile Glu Ala Lys Gly Arg Met Lys Arg Gly Asn Lys 
        435                 440                 445             


Val Trp Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 
    450                 455                 460                 


Trp Val Ala Leu Arg Asn Val Lys Ala Ser Thr Asn Ser Pro Trp Glu 
465                 470                 475                 480 


His Cys Ile Asp Arg Tyr Pro Asp Ala Ile Asp Ser Asp Ser Gly Lys 
                485                 490                 495     


Ser Glu Thr Arg Val Gln Asn Gly Arg Ser 
            500                 505     


<210>  3
<211>  1517
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  modified Thlaspi arvense FAE1 sequence

<400>  3
atgacgtccg ttaacgttaa gctcctttac cattacgtca tcaccaactt tttcaacctt       60

tgcttcttcc cgttagcggc gatcgttgcc ggaaaagcct ctcggcttac cacaaacgat      120

cttcaccact tctactattc ctatctccaa cacaacctaa taaccatatc tctactcttt      180

gccttcaccg ttttcggttt ggctctctac atacccggcc caaaccggtt tacctcgttg      240

accattcctg ctaccttcca ccatcgcatc ttagaagcag tatctctaag gtcatggata      300

tcttctatca agtaagatta gccgatcctt tacggaacgc ggcaagcgat gattcgtcct      360

ggcttgattt cttgaggaag attcaggagc ggtctggtct aggcgatgaa acccacggcc      420

ccgagggact gcttcaggtc cctccacgga agacttttgc cgcggcgcgt gaagaaacag      480

agcaagtgat catcggtgcg ctcgaaaaac tattcgagaa caccaaagtt aaccctaaag      540

agattggtat acttgtggtg aactcaagca tgtttaatcc gactccttcg ctctcggcga      600

tggttgttaa tactttcaag ctccgaagca acatcagaag ctttaatctt ggaggaatgg      660

gttgtagtgc cggcgttata gccattgatc tggctaagga cttgttgcat gtccataaaa      720

acacttatgc tcttgtggtg agcacagaga acatcactta caacatttat gctggtgata      780

acagatccat gatggtttcg aattgcttgt tccgtgttgg tggggccgcg attttgctct      840

ccaacaagcc gagggaccgg agacggtcca agtaccagct acttcacacg gttcggacgc      900

ataccggagc tgacgacaag tctttccgat gtgtgcaaca agaagacgac gagagcggta      960

aaaccggggt gtgtttgtcc aaggacataa ccggtgttgc cgggagaact gttcagaaaa     1020

acataacaac attgggtccg ttggttcttc cttttagcga gaaatttctt tttttcgtta     1080

ccttcatcgc caagaaactc tttaaagaca agatcaaaca ttactacgtc ccggatttca     1140

agcttgctat cgaccatttt tgtattcatg ccggaggcag agccgtgatc gatgtgctac     1200

agaagaactt aggtctattg ccgatcgatg tggaggcatc taggtcaacg ttacatagat     1260

ttgggaacac ttcgtctagc tcaatttggt atgaattggc gtacatagag gcaaaaggaa     1320

ggatgaagag agggaacaaa gtttggcaga ttgctttagg gtcagggttt aagtgtaata     1380

gtgcggtttg ggtggctcta cgcaatgtca aggcttcgac aaatagtcct tgggaacatt     1440

gcattgatag atatccagat gcaattgatt ctgattcggg taagtcagag actcgtgtcc     1500

aaaacggtcg gtcctaa                                                    1517


<210>  4
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified Thlaspi arvense FAE1 sequence

<400>  4

Met Thr Ser Val Asn Val Lys Leu Leu Tyr His Tyr Val Ile Thr Asn 
1               5                   10                  15      


Phe Phe Asn Leu Cys Phe Phe Pro Leu Ala Ala Ile Val Ala Gly Lys 
            20                  25                  30          


Ala Ser Arg Leu Thr Thr Asn Asp Leu His His Phe Tyr Tyr Ser Tyr 
        35                  40                  45              


Leu Gln His Asn Leu Ile Thr Ile Ser Leu Leu Phe Ala Phe Thr Val 
    50                  55                  60                  


Phe Gly Leu Ala Leu Tyr Ile Pro Gly Pro Asn Arg Phe Thr Ser Leu 
65                  70                  75                  80  


Thr Ile Pro Ala Thr Phe His His Arg Ile Leu Glu Ala Val Ser Leu 
                85                  90                  95      


Arg Ser Trp Ile Ser Ser Ile Lys 
            100                 


<210>  5
<211>  1522
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  modified Thlaspi arvense FAE1 sequence

<400>  5
atgacgtccg ttaacgttaa gctcctttac cattacgtca tcaccaactt tttcaacctt       60

tgcttcttcc cgttagcggc gatcgttgcc ggaaaagcct ctcggcttac cacaaacgat      120

cttcaccact tctactattc ctatctccaa cacaacctaa taaccatatc tctactcttt      180

gccttcaccg ttttcggttt ggctctctac atcgtaaacc cggcccaaac cggtttacct      240

cgttgaccat tcctgctacc ttccaccatc gcatcttaga agcagtatct ctaaggtcat      300

ggatatcttc tatcaagtaa gattagccga tcctttacgg aacgcggcaa gcgatgattc      360

gtcctggctt gatttcttga ggaagattca ggagcggtct ggtctaggcg atgaaaccca      420

cggccccgag ggactgcttc aggtccctcc acggaagact tttgccgcgg cgcgtgaaga      480

aacagagcaa gtgatcatcg gtgcgctcga aaaactattc gagaacacca aagttaaccc      540

taaagagatt ggtatacttg tggtgaactc aagcatgttt aatccgactc cttcgctctc      600

ggcgatggtt gttaatactt tcaagctccg aagcaacatc agaagcttta atcttggagg      660

aatgggttgt agtgccggcg ttatagccat tgatctggct aaggacttgt tgcatgtcca      720

taaaaacact tatgctcttg tggtgagcac agagaacatc acttacaaca tttatgctgg      780

tgataacaga tccatgatgg tttcgaattg cttgttccgt gttggtgggg ccgcgatttt      840

gctctccaac aagccgaggg accggagacg gtccaagtac cagctacttc acacggttcg      900

gacgcatacc ggagctgacg acaagtcttt ccgatgtgtg caacaagaag acgacgagag      960

cggtaaaacc ggggtgtgtt tgtccaagga cataaccggt gttgccggga gaactgttca     1020

gaaaaacata acaacattgg gtccgttggt tcttcctttt agcgagaaat ttcttttttt     1080

cgttaccttc atcgccaaga aactctttaa agacaagatc aaacattact acgtcccgga     1140

tttcaagctt gctatcgacc atttttgtat tcatgccgga ggcagagccg tgatcgatgt     1200

gctacagaag aacttaggtc tattgccgat cgatgtggag gcatctaggt caacgttaca     1260

tagatttggg aacacttcgt ctagctcaat ttggtatgaa ttggcgtaca tagaggcaaa     1320

aggaaggatg aagagaggga acaaagtttg gcagattgct ttagggtcag ggtttaagtg     1380

taatagtgcg gtttgggtgg ctctacgcaa tgtcaaggct tcgacaaata gtccttggga     1440

acattgcatt gatagatatc cagatgcaat tgattctgat tcgggtaagt cagagactcg     1500

tgtccaaaac ggtcggtcct aa                                              1522


<210>  6
<211>  81
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified Thlaspi arvense FAE1 sequence

<400>  6

Met Thr Ser Val Asn Val Lys Leu Leu Tyr His Tyr Val Ile Thr Asn 
1               5                   10                  15      


Phe Phe Asn Leu Cys Phe Phe Pro Leu Ala Ala Ile Val Ala Gly Lys 
            20                  25                  30          


Ala Ser Arg Leu Thr Thr Asn Asp Leu His His Phe Tyr Tyr Ser Tyr 
        35                  40                  45              


Leu Gln His Asn Leu Ile Thr Ile Ser Leu Leu Phe Ala Phe Thr Val 
    50                  55                  60                  


Phe Gly Leu Ala Leu Tyr Ile Val Asn Pro Ala Gln Thr Gly Leu Pro 
65                  70                  75                  80  


Arg 
    


<210>  7
<211>  1522
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  modified Thlaspi arvense FAE1 sequence

<400>  7
atgacgtccg ttaacgttaa gctcctttac cattacgtca tcaccaactt tttcaacctt       60

tgcttcttcc cgttagcggc gatcgttgcc ggaaaagcct ctcggcttac cacaaacgat      120

cttcaccact tctactattc ctatctccaa cacaacctaa taaccatatc tctactcttt      180

gccttcaccg ttttcggttt ggctctctac atcgtatacc cggcccaaac cggtttacct      240

cgttgaccat tcctgctacc ttccaccatc gcatcttaga agcagtatct ctaaggtcat      300

ggatatcttc tatcaagtaa gattagccga tcctttacgg aacgcggcaa gcgatgattc      360

gtcctggctt gatttcttga ggaagattca ggagcggtct ggtctaggcg atgaaaccca      420

cggccccgag ggactgcttc aggtccctcc acggaagact tttgccgcgg cgcgtgaaga      480

aacagagcaa gtgatcatcg gtgcgctcga aaaactattc gagaacacca aagttaaccc      540

taaagagatt ggtatacttg tggtgaactc aagcatgttt aatccgactc cttcgctctc      600

ggcgatggtt gttaatactt tcaagctccg aagcaacatc agaagcttta atcttggagg      660

aatgggttgt agtgccggcg ttatagccat tgatctggct aaggacttgt tgcatgtcca      720

taaaaacact tatgctcttg tggtgagcac agagaacatc acttacaaca tttatgctgg      780

tgataacaga tccatgatgg tttcgaattg cttgttccgt gttggtgggg ccgcgatttt      840

gctctccaac aagccgaggg accggagacg gtccaagtac cagctacttc acacggttcg      900

gacgcatacc ggagctgacg acaagtcttt ccgatgtgtg caacaagaag acgacgagag      960

cggtaaaacc ggggtgtgtt tgtccaagga cataaccggt gttgccggga gaactgttca     1020

gaaaaacata acaacattgg gtccgttggt tcttcctttt agcgagaaat ttcttttttt     1080

cgttaccttc atcgccaaga aactctttaa agacaagatc aaacattact acgtcccgga     1140

tttcaagctt gctatcgacc atttttgtat tcatgccgga ggcagagccg tgatcgatgt     1200

gctacagaag aacttaggtc tattgccgat cgatgtggag gcatctaggt caacgttaca     1260

tagatttggg aacacttcgt ctagctcaat ttggtatgaa ttggcgtaca tagaggcaaa     1320

aggaaggatg aagagaggga acaaagtttg gcagattgct ttagggtcag ggtttaagtg     1380

taatagtgcg gtttgggtgg ctctacgcaa tgtcaaggct tcgacaaata gtccttggga     1440

acattgcatt gatagatatc cagatgcaat tgattctgat tcgggtaagt cagagactcg     1500

tgtccaaaac ggtcggtcct aa                                              1522


<210>  8
<211>  81
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified Thlaspi arvense FAE1 sequence

<400>  8

Met Thr Ser Val Asn Val Lys Leu Leu Tyr His Tyr Val Ile Thr Asn 
1               5                   10                  15      


Phe Phe Asn Leu Cys Phe Phe Pro Leu Ala Ala Ile Val Ala Gly Lys 
            20                  25                  30          


Ala Ser Arg Leu Thr Thr Asn Asp Leu His His Phe Tyr Tyr Ser Tyr 
        35                  40                  45              


Leu Gln His Asn Leu Ile Thr Ile Ser Leu Leu Phe Ala Phe Thr Val 
    50                  55                  60                  


Phe Gly Leu Ala Leu Tyr Ile Val Asn Pro Ala Gln Thr Gly Leu Pro 
65                  70                  75                  80  


Arg 
    


<210>  9
<211>  1520
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  modified Thlaspi arvense FAE1 sequence

<400>  9
atgacgtccg ttaacgttaa gctcctttac cattacgtca tcaccaactt tttcaacctt       60

tgcttcttcc cgttagcggc gatcgttgcc ggaaaagcct ctcggcttac cacaaacgat      120

cttcaccact tctactattc ctatctccaa cacaacctaa taaccatatc tctactcttt      180

gccttcaccg ttttcggttt ggctctctac atcgtacccg gcccaaaccg gtttacctcg      240

ttgaccattc ctgctacctt ccaccatcgc atcttagaag cagtatctct aaggtcatgg      300

atatcttcta tcaagtaaga ttagccgatc ctttacggaa cgcggcaagc gatgattcgt      360

cctggcttga tttcttgagg aagattcagg agcggtctgg tctaggcgat gaaacccacg      420

gccccgaggg actgcttcag gtccctccac ggaagacttt tgccgcggcg cgtgaagaaa      480

cagagcaagt gatcatcggt gcgctcgaaa aactattcga gaacaccaaa gttaacccta      540

aagagattgg tatacttgtg gtgaactcaa gcatgtttaa tccgactcct tcgctctcgg      600

cgatggttgt taatactttc aagctccgaa gcaacatcag aagctttaat cttggaggaa      660

tgggttgtag tgccggcgtt atagccattg atctggctaa ggacttgttg catgtccata      720

aaaacactta tgctcttgtg gtgagcacag agaacatcac ttacaacatt tatgctggtg      780

ataacagatc catgatggtt tcgaattgct tgttccgtgt tggtggggcc gcgattttgc      840

tctccaacaa gccgagggac cggagacggt ccaagtacca gctacttcac acggttcgga      900

cgcataccgg agctgacgac aagtctttcc gatgtgtgca acaagaagac gacgagagcg      960

gtaaaaccgg ggtgtgtttg tccaaggaca taaccggtgt tgccgggaga actgttcaga     1020

aaaacataac aacattgggt ccgttggttc ttccttttag cgagaaattt ctttttttcg     1080

ttaccttcat cgccaagaaa ctctttaaag acaagatcaa acattactac gtcccggatt     1140

tcaagcttgc tatcgaccat ttttgtattc atgccggagg cagagccgtg atcgatgtgc     1200

tacagaagaa cttaggtcta ttgccgatcg atgtggaggc atctaggtca acgttacata     1260

gatttgggaa cacttcgtct agctcaattt ggtatgaatt ggcgtacata gaggcaaaag     1320

gaaggatgaa gagagggaac aaagtttggc agattgcttt agggtcaggg tttaagtgta     1380

atagtgcggt ttgggtggct ctacgcaatg tcaaggcttc gacaaatagt ccttgggaac     1440

attgcattga tagatatcca gatgcaattg attctgattc gggtaagtca gagactcgtg     1500

tccaaaacgg tcggtcctaa                                                 1520


<210>  10
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified Thlaspi arvense FAE1 sequence

<400>  10

Met Thr Ser Val Asn Val Lys Leu Leu Tyr His Tyr Val Ile Thr Asn 
1               5                   10                  15      


Phe Phe Asn Leu Cys Phe Phe Pro Leu Ala Ala Ile Val Ala Gly Lys 
            20                  25                  30          


Ala Ser Arg Leu Thr Thr Asn Asp Leu His His Phe Tyr Tyr Ser Tyr 
        35                  40                  45              


Leu Gln His Asn Leu Ile Thr Ile Ser Leu Leu Phe Ala Phe Thr Val 
    50                  55                  60                  


Phe Gly Leu Ala Leu Tyr Ile Pro Gly Pro Asn Arg Phe Thr Ser Leu 
65                  70                  75                  80  


Thr Ile Pro Ala Thr Phe His His Arg Ile Leu Glu Ala Val Ser Leu 
                85                  90                  95      


Arg Ser Trp Ile Ser Ser Ile Lys 
            100                 


<210>  11
<211>  1521
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  modified Thlaspi arvense FAE1 sequence

<400>  11
atgacgtccg ttaacgttaa gctcctttac cattacgtca tcaccaactt tttcaacctt       60

tgcttcttcc cgttagcggc gatcgttgcc ggaaaagcct ctcggcttac cacaaacgat      120

cttcaccact tctactattc ctatctccaa cacaacctaa taaccatatc tctactcttt      180

gccttcaccg ttttcggttt ggctctctac atcgtaaccc ggcccaaacc ggtttacctc      240

gttgaccatt cctgctacct tccaccatcg catcttagaa gcagtatctc taaggtcatg      300

gatatcttct atcaagtaag attagccgat cctttacgga acgcggcaag cgatgattcg      360

tcctggcttg atttcttgag gaagattcag gagcggtctg gtctaggcga tgaaacccac      420

ggccccgagg gactgcttca ggtccctcca cggaagactt ttgccgcggc gcgtgaagaa      480

acagagcaag tgatcatcgg tgcgctcgaa aaactattcg agaacaccaa agttaaccct      540

aaagagattg gtatacttgt ggtgaactca agcatgttta atccgactcc ttcgctctcg      600

gcgatggttg ttaatacttt caagctccga agcaacatca gaagctttaa tcttggagga      660

atgggttgta gtgccggcgt tatagccatt gatctggcta aggacttgtt gcatgtccat      720

aaaaacactt atgctcttgt ggtgagcaca gagaacatca cttacaacat ttatgctggt      780

gataacagat ccatgatggt ttcgaattgc ttgttccgtg ttggtggggc cgcgattttg      840

ctctccaaca agccgaggga ccggagacgg tccaagtacc agctacttca cacggttcgg      900

acgcataccg gagctgacga caagtctttc cgatgtgtgc aacaagaaga cgacgagagc      960

ggtaaaaccg gggtgtgttt gtccaaggac ataaccggtg ttgccgggag aactgtttag     1020

aaaaacataa caacattggg tccgttggtt cttcctttta gcgagaaatt tctttttttc     1080

gttaccttca tcgccaagaa actctttaaa gacaagatca aacattacta cgtcccggat     1140

ttcaagcttg ctatcgacca tttttgtatt catgccggag gcagagccgt gatcgatgtg     1200

ctacagaaga acttaggtct attgccgatc gatgtggagg catctaggtc aacgttacat     1260

agatttggga acacttcgtc tagctcaatt tggtatgaat tggcgtacat agaggcaaaa     1320

ggaaggatga agagagggaa caaagtttgg cagattgctt tagggtcagg gtttaagtgt     1380

aatagtgcgg tttgggtggc tctacgcaat gtcaaggctt cgacaaatag tccttgggaa     1440

cattgcattg atagatatcc agatgcaatt gattctgatt cgggtaagtc agagactcgt     1500

gtccaaaacg gtcggtccta a                                               1521


<210>  12
<211>  339
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified Thlaspi arvense FAE1 sequence

<400>  12

Met Thr Ser Val Asn Val Lys Leu Leu Tyr His Tyr Val Ile Thr Asn 
1               5                   10                  15      


Phe Phe Asn Leu Cys Phe Phe Pro Leu Ala Ala Ile Val Ala Gly Lys 
            20                  25                  30          


Ala Ser Arg Leu Thr Thr Asn Asp Leu His His Phe Tyr Tyr Ser Tyr 
        35                  40                  45              


Leu Gln His Asn Leu Ile Thr Ile Ser Leu Leu Phe Ala Phe Thr Val 
    50                  55                  60                  


Phe Gly Leu Ala Leu Tyr Ile Val Thr Arg Pro Lys Pro Val Tyr Leu 
65                  70                  75                  80  


Val Asp His Ser Cys Tyr Leu Pro Pro Ser His Leu Arg Ser Ser Ile 
                85                  90                  95      


Ser Lys Val Met Asp Ile Phe Tyr Gln Val Arg Leu Ala Asp Pro Leu 
            100                 105                 110         


Arg Asn Ala Ala Ser Asp Asp Ser Ser Trp Leu Asp Phe Leu Arg Lys 
        115                 120                 125             


Ile Gln Glu Arg Ser Gly Leu Gly Asp Glu Thr His Gly Pro Glu Gly 
    130                 135                 140                 


Leu Leu Gln Val Pro Pro Arg Lys Thr Phe Ala Ala Ala Arg Glu Glu 
145                 150                 155                 160 


Thr Glu Gln Val Ile Ile Gly Ala Leu Glu Lys Leu Phe Glu Asn Thr 
                165                 170                 175     


Lys Val Asn Pro Lys Glu Ile Gly Ile Leu Val Val Asn Ser Ser Met 
            180                 185                 190         


Phe Asn Pro Thr Pro Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys 
        195                 200                 205             


Leu Arg Ser Asn Ile Arg Ser Phe Asn Leu Gly Gly Met Gly Cys Ser 
    210                 215                 220                 


Ala Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu His Val His 
225                 230                 235                 240 


Lys Asn Thr Tyr Ala Leu Val Val Ser Thr Glu Asn Ile Thr Tyr Asn 
                245                 250                 255     


Ile Tyr Ala Gly Asp Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe 
            260                 265                 270         


Arg Val Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro Arg Asp Arg 
        275                 280                 285             


Arg Arg Ser Lys Tyr Gln Leu Leu His Thr Val Arg Thr His Thr Gly 
    290                 295                 300                 


Ala Asp Asp Lys Ser Phe Arg Cys Val Gln Gln Glu Asp Asp Glu Ser 
305                 310                 315                 320 


Gly Lys Thr Gly Val Cys Leu Ser Lys Asp Ile Thr Gly Val Ala Gly 
                325                 330                 335     


Arg Thr Val 
            


<210>  13
<211>  1521
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  modified Thlaspi arvense FAE1 sequence

<400>  13
atgacgtccg ttaacgttaa gctcctttac cattacgtca tcaccaactt tttcaacctt       60

tgcttcttcc cgttagcggc gatcgttgcc ggaaaagcct ctcggcttac cacaaacgat      120

cttcaccact tctactattc ctatctccaa cacaacctaa taaccatatc tctactcttt      180

gccttcaccg ttttcggttt ggctctctac atcgtaaccc ggcccaaacc ggtttacctc      240

gttgaccatt cctgctacct tccaccatcg catcttagaa gcagtatctc taaggtcatg      300

gatatcttct atcaagtaag attagccgat cctttacgga acgcggcaag cgatgattcg      360

tcctggcttg atttcttgag gaagattcag gagcggtctg gtctaggcga tgaaacccac      420

ggccccgagg gactgcttca ggtccctcca cggaagactt ttgccgcggc gcgtgaagaa      480

acagagcaag tgatcatcgg tgcgctcgaa aaactattcg agaacaccaa agttaaccct      540

aaagagattg gtatacttgt ggtgaactca agcatgttta atccgactcc ttcgctctcg      600

gcgatggttg ttaatacttt caagctccga agcaacatca gaagctttaa tcttggagga      660

atgggttgta gtgccggcgt tatagccatt gatctggcta aggacttgtt gcatgtccat      720

aaaaacactt atgctcttgt ggtgagcaca gagaacatca cttacaacat ttatgctggt      780

gataacagat ccatgatggt ttcgaattgc ttgttccgtg ttggtggggc cgcgattttg      840

ctctccaaca agccgaggga ccggagacgg tccaagtacc agctacttca cacggttcgg      900

acgcataccg gagctgacga caagtctttc cgatgtgtgc aacaagaaga cgacgagagc      960

ggtaaaaccg gggtgtgttt gtccaaggac ataaccggtg ttgccgggag aactgttcag     1020

aaaaacataa caacattggg tccgttggtt cttcctttta gcgagaaatt tctttttttc     1080

gttaccttca tcgccaagaa actctttaaa gacaagatca aacattacta cgtcccggat     1140

ttcaagcttg ctatcgacca tttttgtatt catgccggag gcagagccgt gatcgatgtg     1200

ctacagaaga acttaggtct attgccgatc gatgtggagg catctaggtc aacgttacat     1260

agatttggga acacttcgtc tagctcaatt tggtatgaat tggcgtacat agaggcaaaa     1320

ggaaggatga agagagggaa caaagtttag cagattgctt tagggtcagg gtttaagtgt     1380

aatagtgcgg tttgggtggc tctacgcaat gtcaaggctt cgacaaatag tccttgggaa     1440

cattgcattg atagatatcc agatgcaatt gattctgatt cgggtaagtc agagactcgt     1500

gtccaaaacg gtcggtccta a                                               1521


<210>  14
<211>  449
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified Thlaspi arvense FAE1 sequence

<400>  14

Met Thr Ser Val Asn Val Lys Leu Leu Tyr His Tyr Val Ile Thr Asn 
1               5                   10                  15      


Phe Phe Asn Leu Cys Phe Phe Pro Leu Ala Ala Ile Val Ala Gly Lys 
            20                  25                  30          


Ala Ser Arg Leu Thr Thr Asn Asp Leu His His Phe Tyr Tyr Ser Tyr 
        35                  40                  45              


Leu Gln His Asn Leu Ile Thr Ile Ser Leu Leu Phe Ala Phe Thr Val 
    50                  55                  60                  


Phe Gly Leu Ala Leu Tyr Ile Val Thr Arg Pro Lys Pro Val Tyr Leu 
65                  70                  75                  80  


Val Asp His Ser Cys Tyr Leu Pro Pro Ser His Leu Arg Ser Ser Ile 
                85                  90                  95      


Ser Lys Val Met Asp Ile Phe Tyr Gln Val Arg Leu Ala Asp Pro Leu 
            100                 105                 110         


Arg Asn Ala Ala Ser Asp Asp Ser Ser Trp Leu Asp Phe Leu Arg Lys 
        115                 120                 125             


Ile Gln Glu Arg Ser Gly Leu Gly Asp Glu Thr His Gly Pro Glu Gly 
    130                 135                 140                 


Leu Leu Gln Val Pro Pro Arg Lys Thr Phe Ala Ala Ala Arg Glu Glu 
145                 150                 155                 160 


Thr Glu Gln Val Ile Ile Gly Ala Leu Glu Lys Leu Phe Glu Asn Thr 
                165                 170                 175     


Lys Val Asn Pro Lys Glu Ile Gly Ile Leu Val Val Asn Ser Ser Met 
            180                 185                 190         


Phe Asn Pro Thr Pro Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys 
        195                 200                 205             


Leu Arg Ser Asn Ile Arg Ser Phe Asn Leu Gly Gly Met Gly Cys Ser 
    210                 215                 220                 


Ala Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu His Val His 
225                 230                 235                 240 


Lys Asn Thr Tyr Ala Leu Val Val Ser Thr Glu Asn Ile Thr Tyr Asn 
                245                 250                 255     


Ile Tyr Ala Gly Asp Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe 
            260                 265                 270         


Arg Val Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro Arg Asp Arg 
        275                 280                 285             


Arg Arg Ser Lys Tyr Gln Leu Leu His Thr Val Arg Thr His Thr Gly 
    290                 295                 300                 


Ala Asp Asp Lys Ser Phe Arg Cys Val Gln Gln Glu Asp Asp Glu Ser 
305                 310                 315                 320 


Gly Lys Thr Gly Val Cys Leu Ser Lys Asp Ile Thr Gly Val Ala Gly 
                325                 330                 335     


Arg Thr Val Gln Lys Asn Ile Thr Thr Leu Gly Pro Leu Val Leu Pro 
            340                 345                 350         


Phe Ser Glu Lys Phe Leu Phe Phe Val Thr Phe Ile Ala Lys Lys Leu 
        355                 360                 365             


Phe Lys Asp Lys Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 
    370                 375                 380                 


Ile Asp His Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp Val 
385                 390                 395                 400 


Leu Gln Lys Asn Leu Gly Leu Leu Pro Ile Asp Val Glu Ala Ser Arg 
                405                 410                 415     


Ser Thr Leu His Arg Phe Gly Asn Thr Ser Ser Ser Ser Ile Trp Tyr 
            420                 425                 430         


Glu Leu Ala Tyr Ile Glu Ala Lys Gly Arg Met Lys Arg Gly Asn Lys 
        435                 440                 445             


Val 
    


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guide sequence that can be used to direct a Cas nuclease to the 
       FAE1 gene

<400>  15
tggctctcta catcgtaacc                                                   20


<210>  16
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer

<400>  16
attgtggctc tctacatcgt aacc                                              24


<210>  17
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer

<400>  17
aaacggttac gatgtagaga gcca                                              24


<210>  18
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer

<400>  18
acatgcatgt aaaacgtaac gg                                                22


<210>  19
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer

<400>  19
tggattatat caggatgtgg cg                                                22


