                         Sequence_Listing_13542-039-228.txt

<110>  Cheung, Nai-Kong V.
       Lopez-Albaitero, Andres
       Xu, Hong
 
<120>  Bispecific HER2 and CD3 Binding Molecules

<130>  13542-039-228

<140>  to be assigned
<141>  On even date herewith

<150>  PCT/US2015/041989
<151>  2015-07-24

<150>  US 62/029,342
<151>  2014-07-25

<160>  72    

<170>  PatentIn version 3.5

<210>  1
<211>  4664
<212>  DNA
<213>  Homo sapiens

<300>
<308>  NM_004448.3
<309>  2014-05-26
<313>  (1)..(4664)

<400>  1
gcttgctccc aatcacagga gaaggaggag gtggaggagg agggctgctt gaggaagtat       60

aagaatgaag ttgtgaagct gagattcccc tccattggga ccggagaaac caggggagcc      120

ccccgggcag ccgcgcgccc cttcccacgg ggccctttac tgcgccgcgc gcccggcccc      180

cacccctcgc agcaccccgc gccccgcgcc ctcccagccg ggtccagccg gagccatggg      240

gccggagccg cagtgagcac catggagctg gcggccttgt gccgctgggg gctcctcctc      300

gccctcttgc cccccggagc cgcgagcacc caagtgtgca ccggcacaga catgaagctg      360

cggctccctg ccagtcccga gacccacctg gacatgctcc gccacctcta ccagggctgc      420

caggtggtgc agggaaacct ggaactcacc tacctgccca ccaatgccag cctgtccttc      480

ctgcaggata tccaggaggt gcagggctac gtgctcatcg ctcacaacca agtgaggcag      540

gtcccactgc agaggctgcg gattgtgcga ggcacccagc tctttgagga caactatgcc      600

ctggccgtgc tagacaatgg agacccgctg aacaatacca cccctgtcac aggggcctcc      660

ccaggaggcc tgcgggagct gcagcttcga agcctcacag agatcttgaa aggaggggtc      720

ttgatccagc ggaaccccca gctctgctac caggacacga ttttgtggaa ggacatcttc      780

cacaagaaca accagctggc tctcacactg atagacacca accgctctcg ggcctgccac      840

ccctgttctc cgatgtgtaa gggctcccgc tgctggggag agagttctga ggattgtcag      900

agcctgacgc gcactgtctg tgccggtggc tgtgcccgct gcaaggggcc actgcccact      960

gactgctgcc atgagcagtg tgctgccggc tgcacgggcc ccaagcactc tgactgcctg     1020

gcctgcctcc acttcaacca cagtggcatc tgtgagctgc actgcccagc cctggtcacc     1080

tacaacacag acacgtttga gtccatgccc aatcccgagg gccggtatac attcggcgcc     1140

agctgtgtga ctgcctgtcc ctacaactac ctttctacgg acgtgggatc ctgcaccctc     1200

gtctgccccc tgcacaacca agaggtgaca gcagaggatg gaacacagcg gtgtgagaag     1260

tgcagcaagc cctgtgcccg agtgtgctat ggtctgggca tggagcactt gcgagaggtg     1320

agggcagtta ccagtgccaa tatccaggag tttgctggct gcaagaagat ctttgggagc     1380

ctggcatttc tgccggagag ctttgatggg gacccagcct ccaacactgc cccgctccag     1440

ccagagcagc tccaagtgtt tgagactctg gaagagatca caggttacct atacatctca     1500

gcatggccgg acagcctgcc tgacctcagc gtcttccaga acctgcaagt aatccgggga     1560

cgaattctgc acaatggcgc ctactcgctg accctgcaag ggctgggcat cagctggctg     1620

gggctgcgct cactgaggga actgggcagt ggactggccc tcatccacca taacacccac     1680

ctctgcttcg tgcacacggt gccctgggac cagctctttc ggaacccgca ccaagctctg     1740

ctccacactg ccaaccggcc agaggacgag tgtgtgggcg agggcctggc ctgccaccag     1800

ctgtgcgccc gagggcactg ctggggtcca gggcccaccc agtgtgtcaa ctgcagccag     1860

ttccttcggg gccaggagtg cgtggaggaa tgccgagtac tgcaggggct ccccagggag     1920

tatgtgaatg ccaggcactg tttgccgtgc caccctgagt gtcagcccca gaatggctca     1980

gtgacctgtt ttggaccgga ggctgaccag tgtgtggcct gtgcccacta taaggaccct     2040

cccttctgcg tggcccgctg ccccagcggt gtgaaacctg acctctccta catgcccatc     2100

tggaagtttc cagatgagga gggcgcatgc cagccttgcc ccatcaactg cacccactcc     2160

tgtgtggacc tggatgacaa gggctgcccc gccgagcaga gagccagccc tctgacgtcc     2220

atcatctctg cggtggttgg cattctgctg gtcgtggtct tgggggtggt ctttgggatc     2280

ctcatcaagc gacggcagca gaagatccgg aagtacacga tgcggagact gctgcaggaa     2340

acggagctgg tggagccgct gacacctagc ggagcgatgc ccaaccaggc gcagatgcgg     2400

atcctgaaag agacggagct gaggaaggtg aaggtgcttg gatctggcgc ttttggcaca     2460

gtctacaagg gcatctggat ccctgatggg gagaatgtga aaattccagt ggccatcaaa     2520

gtgttgaggg aaaacacatc ccccaaagcc aacaaagaaa tcttagacga agcatacgtg     2580

atggctggtg tgggctcccc atatgtctcc cgccttctgg gcatctgcct gacatccacg     2640

gtgcagctgg tgacacagct tatgccctat ggctgcctct tagaccatgt ccgggaaaac     2700

cgcggacgcc tgggctccca ggacctgctg aactggtgta tgcagattgc caaggggatg     2760

agctacctgg aggatgtgcg gctcgtacac agggacttgg ccgctcggaa cgtgctggtc     2820

aagagtccca accatgtcaa aattacagac ttcgggctgg ctcggctgct ggacattgac     2880

gagacagagt accatgcaga tgggggcaag gtgcccatca agtggatggc gctggagtcc     2940

attctccgcc ggcggttcac ccaccagagt gatgtgtgga gttatggtgt gactgtgtgg     3000

gagctgatga cttttggggc caaaccttac gatgggatcc cagcccggga gatccctgac     3060

ctgctggaaa agggggagcg gctgccccag ccccccatct gcaccattga tgtctacatg     3120

atcatggtca aatgttggat gattgactct gaatgtcggc caagattccg ggagttggtg     3180

tctgaattct cccgcatggc cagggacccc cagcgctttg tggtcatcca gaatgaggac     3240

ttgggcccag ccagtccctt ggacagcacc ttctaccgct cactgctgga ggacgatgac     3300

atgggggacc tggtggatgc tgaggagtat ctggtacccc agcagggctt cttctgtcca     3360

gaccctgccc cgggcgctgg gggcatggtc caccacaggc accgcagctc atctaccagg     3420

agtggcggtg gggacctgac actagggctg gagccctctg aagaggaggc ccccaggtct     3480

ccactggcac cctccgaagg ggctggctcc gatgtatttg atggtgacct gggaatgggg     3540

gcagccaagg ggctgcaaag cctccccaca catgacccca gccctctaca gcggtacagt     3600

gaggacccca cagtacccct gccctctgag actgatggct acgttgcccc cctgacctgc     3660

agcccccagc ctgaatatgt gaaccagcca gatgttcggc cccagccccc ttcgccccga     3720

gagggccctc tgcctgctgc ccgacctgct ggtgccactc tggaaaggcc caagactctc     3780

tccccaggga agaatggggt cgtcaaagac gtttttgcct ttgggggtgc cgtggagaac     3840

cccgagtact tgacacccca gggaggagct gcccctcagc cccaccctcc tcctgccttc     3900

agcccagcct tcgacaacct ctattactgg gaccaggacc caccagagcg gggggctcca     3960

cccagcacct tcaaagggac acctacggca gagaacccag agtacctggg tctggacgtg     4020

ccagtgtgaa ccagaaggcc aagtccgcag aagccctgat gtgtcctcag ggagcaggga     4080

aggcctgact tctgctggca tcaagaggtg ggagggccct ccgaccactt ccaggggaac     4140

ctgccatgcc aggaacctgt cctaaggaac cttccttcct gcttgagttc ccagatggct     4200

ggaaggggtc cagcctcgtt ggaagaggaa cagcactggg gagtctttgt ggattctgag     4260

gccctgccca atgagactct agggtccagt ggatgccaca gcccagcttg gccctttcct     4320

tccagatcct gggtactgaa agccttaggg aagctggcct gagaggggaa gcggccctaa     4380

gggagtgtct aagaacaaaa gcgacccatt cagagactgt ccctgaaacc tagtactgcc     4440

ccccatgagg aaggaacagc aatggtgtca gtatccaggc tttgtacaga gtgcttttct     4500

gtttagtttt tacttttttt gttttgtttt tttaaagatg aaataaagac ccagggggag     4560

aatgggtgtt gtatggggag gcaagtgtgg ggggtccttc tccacaccca ctttgtccat     4620

ttgcaaatat attttggaaa acagctaaaa aaaaaaaaaa aaaa                      4664


<210>  2
<211>  1255
<212>  PRT
<213>  Homo sapiens

<300>
<308>  NP_004439.2
<309>  2014-05-26
<313>  (1)..(1255)

<400>  2

Met Glu Leu Ala Ala Leu Cys Arg Trp Gly Leu Leu Leu Ala Leu Leu 
1               5                   10                  15      


Pro Pro Gly Ala Ala Ser Thr Gln Val Cys Thr Gly Thr Asp Met Lys 
            20                  25                  30          


Leu Arg Leu Pro Ala Ser Pro Glu Thr His Leu Asp Met Leu Arg His 
        35                  40                  45              


Leu Tyr Gln Gly Cys Gln Val Val Gln Gly Asn Leu Glu Leu Thr Tyr 
    50                  55                  60                  


Leu Pro Thr Asn Ala Ser Leu Ser Phe Leu Gln Asp Ile Gln Glu Val 
65                  70                  75                  80  


Gln Gly Tyr Val Leu Ile Ala His Asn Gln Val Arg Gln Val Pro Leu 
                85                  90                  95      


Gln Arg Leu Arg Ile Val Arg Gly Thr Gln Leu Phe Glu Asp Asn Tyr 
            100                 105                 110         


Ala Leu Ala Val Leu Asp Asn Gly Asp Pro Leu Asn Asn Thr Thr Pro 
        115                 120                 125             


Val Thr Gly Ala Ser Pro Gly Gly Leu Arg Glu Leu Gln Leu Arg Ser 
    130                 135                 140                 


Leu Thr Glu Ile Leu Lys Gly Gly Val Leu Ile Gln Arg Asn Pro Gln 
145                 150                 155                 160 


Leu Cys Tyr Gln Asp Thr Ile Leu Trp Lys Asp Ile Phe His Lys Asn 
                165                 170                 175     


Asn Gln Leu Ala Leu Thr Leu Ile Asp Thr Asn Arg Ser Arg Ala Cys 
            180                 185                 190         


His Pro Cys Ser Pro Met Cys Lys Gly Ser Arg Cys Trp Gly Glu Ser 
        195                 200                 205             


Ser Glu Asp Cys Gln Ser Leu Thr Arg Thr Val Cys Ala Gly Gly Cys 
    210                 215                 220                 


Ala Arg Cys Lys Gly Pro Leu Pro Thr Asp Cys Cys His Glu Gln Cys 
225                 230                 235                 240 


Ala Ala Gly Cys Thr Gly Pro Lys His Ser Asp Cys Leu Ala Cys Leu 
                245                 250                 255     


His Phe Asn His Ser Gly Ile Cys Glu Leu His Cys Pro Ala Leu Val 
            260                 265                 270         


Thr Tyr Asn Thr Asp Thr Phe Glu Ser Met Pro Asn Pro Glu Gly Arg 
        275                 280                 285             


Tyr Thr Phe Gly Ala Ser Cys Val Thr Ala Cys Pro Tyr Asn Tyr Leu 
    290                 295                 300                 


Ser Thr Asp Val Gly Ser Cys Thr Leu Val Cys Pro Leu His Asn Gln 
305                 310                 315                 320 


Glu Val Thr Ala Glu Asp Gly Thr Gln Arg Cys Glu Lys Cys Ser Lys 
                325                 330                 335     


Pro Cys Ala Arg Val Cys Tyr Gly Leu Gly Met Glu His Leu Arg Glu 
            340                 345                 350         


Val Arg Ala Val Thr Ser Ala Asn Ile Gln Glu Phe Ala Gly Cys Lys 
        355                 360                 365             


Lys Ile Phe Gly Ser Leu Ala Phe Leu Pro Glu Ser Phe Asp Gly Asp 
    370                 375                 380                 


Pro Ala Ser Asn Thr Ala Pro Leu Gln Pro Glu Gln Leu Gln Val Phe 
385                 390                 395                 400 


Glu Thr Leu Glu Glu Ile Thr Gly Tyr Leu Tyr Ile Ser Ala Trp Pro 
                405                 410                 415     


Asp Ser Leu Pro Asp Leu Ser Val Phe Gln Asn Leu Gln Val Ile Arg 
            420                 425                 430         


Gly Arg Ile Leu His Asn Gly Ala Tyr Ser Leu Thr Leu Gln Gly Leu 
        435                 440                 445             


Gly Ile Ser Trp Leu Gly Leu Arg Ser Leu Arg Glu Leu Gly Ser Gly 
    450                 455                 460                 


Leu Ala Leu Ile His His Asn Thr His Leu Cys Phe Val His Thr Val 
465                 470                 475                 480 


Pro Trp Asp Gln Leu Phe Arg Asn Pro His Gln Ala Leu Leu His Thr 
                485                 490                 495     


Ala Asn Arg Pro Glu Asp Glu Cys Val Gly Glu Gly Leu Ala Cys His 
            500                 505                 510         


Gln Leu Cys Ala Arg Gly His Cys Trp Gly Pro Gly Pro Thr Gln Cys 
        515                 520                 525             


Val Asn Cys Ser Gln Phe Leu Arg Gly Gln Glu Cys Val Glu Glu Cys 
    530                 535                 540                 


Arg Val Leu Gln Gly Leu Pro Arg Glu Tyr Val Asn Ala Arg His Cys 
545                 550                 555                 560 


Leu Pro Cys His Pro Glu Cys Gln Pro Gln Asn Gly Ser Val Thr Cys 
                565                 570                 575     


Phe Gly Pro Glu Ala Asp Gln Cys Val Ala Cys Ala His Tyr Lys Asp 
            580                 585                 590         


Pro Pro Phe Cys Val Ala Arg Cys Pro Ser Gly Val Lys Pro Asp Leu 
        595                 600                 605             


Ser Tyr Met Pro Ile Trp Lys Phe Pro Asp Glu Glu Gly Ala Cys Gln 
    610                 615                 620                 


Pro Cys Pro Ile Asn Cys Thr His Ser Cys Val Asp Leu Asp Asp Lys 
625                 630                 635                 640 


Gly Cys Pro Ala Glu Gln Arg Ala Ser Pro Leu Thr Ser Ile Ile Ser 
                645                 650                 655     


Ala Val Val Gly Ile Leu Leu Val Val Val Leu Gly Val Val Phe Gly 
            660                 665                 670         


Ile Leu Ile Lys Arg Arg Gln Gln Lys Ile Arg Lys Tyr Thr Met Arg 
        675                 680                 685             


Arg Leu Leu Gln Glu Thr Glu Leu Val Glu Pro Leu Thr Pro Ser Gly 
    690                 695                 700                 


Ala Met Pro Asn Gln Ala Gln Met Arg Ile Leu Lys Glu Thr Glu Leu 
705                 710                 715                 720 


Arg Lys Val Lys Val Leu Gly Ser Gly Ala Phe Gly Thr Val Tyr Lys 
                725                 730                 735     


Gly Ile Trp Ile Pro Asp Gly Glu Asn Val Lys Ile Pro Val Ala Ile 
            740                 745                 750         


Lys Val Leu Arg Glu Asn Thr Ser Pro Lys Ala Asn Lys Glu Ile Leu 
        755                 760                 765             


Asp Glu Ala Tyr Val Met Ala Gly Val Gly Ser Pro Tyr Val Ser Arg 
    770                 775                 780                 


Leu Leu Gly Ile Cys Leu Thr Ser Thr Val Gln Leu Val Thr Gln Leu 
785                 790                 795                 800 


Met Pro Tyr Gly Cys Leu Leu Asp His Val Arg Glu Asn Arg Gly Arg 
                805                 810                 815     


Leu Gly Ser Gln Asp Leu Leu Asn Trp Cys Met Gln Ile Ala Lys Gly 
            820                 825                 830         


Met Ser Tyr Leu Glu Asp Val Arg Leu Val His Arg Asp Leu Ala Ala 
        835                 840                 845             


Arg Asn Val Leu Val Lys Ser Pro Asn His Val Lys Ile Thr Asp Phe 
    850                 855                 860                 


Gly Leu Ala Arg Leu Leu Asp Ile Asp Glu Thr Glu Tyr His Ala Asp 
865                 870                 875                 880 


Gly Gly Lys Val Pro Ile Lys Trp Met Ala Leu Glu Ser Ile Leu Arg 
                885                 890                 895     


Arg Arg Phe Thr His Gln Ser Asp Val Trp Ser Tyr Gly Val Thr Val 
            900                 905                 910         


Trp Glu Leu Met Thr Phe Gly Ala Lys Pro Tyr Asp Gly Ile Pro Ala 
        915                 920                 925             


Arg Glu Ile Pro Asp Leu Leu Glu Lys Gly Glu Arg Leu Pro Gln Pro 
    930                 935                 940                 


Pro Ile Cys Thr Ile Asp Val Tyr Met Ile Met Val Lys Cys Trp Met 
945                 950                 955                 960 


Ile Asp Ser Glu Cys Arg Pro Arg Phe Arg Glu Leu Val Ser Glu Phe 
                965                 970                 975     


Ser Arg Met Ala Arg Asp Pro Gln Arg Phe Val Val Ile Gln Asn Glu 
            980                 985                 990         


Asp Leu Gly Pro Ala Ser Pro Leu  Asp Ser Thr Phe Tyr  Arg Ser Leu 
        995                 1000                 1005             


Leu Glu  Asp Asp Asp Met Gly  Asp Leu Val Asp Ala  Glu Glu Tyr 
    1010                 1015                 1020             


Leu Val  Pro Gln Gln Gly Phe  Phe Cys Pro Asp Pro  Ala Pro Gly 
    1025                 1030                 1035             


Ala Gly  Gly Met Val His His  Arg His Arg Ser Ser  Ser Thr Arg 
    1040                 1045                 1050             


Ser Gly  Gly Gly Asp Leu Thr  Leu Gly Leu Glu Pro  Ser Glu Glu 
    1055                 1060                 1065             


Glu Ala  Pro Arg Ser Pro Leu  Ala Pro Ser Glu Gly  Ala Gly Ser 
    1070                 1075                 1080             


Asp Val  Phe Asp Gly Asp Leu  Gly Met Gly Ala Ala  Lys Gly Leu 
    1085                 1090                 1095             


Gln Ser  Leu Pro Thr His Asp  Pro Ser Pro Leu Gln  Arg Tyr Ser 
    1100                 1105                 1110             


Glu Asp  Pro Thr Val Pro Leu  Pro Ser Glu Thr Asp  Gly Tyr Val 
    1115                 1120                 1125             


Ala Pro  Leu Thr Cys Ser Pro  Gln Pro Glu Tyr Val  Asn Gln Pro 
    1130                 1135                 1140             


Asp Val  Arg Pro Gln Pro Pro  Ser Pro Arg Glu Gly  Pro Leu Pro 
    1145                 1150                 1155             


Ala Ala  Arg Pro Ala Gly Ala  Thr Leu Glu Arg Pro  Lys Thr Leu 
    1160                 1165                 1170             


Ser Pro  Gly Lys Asn Gly Val  Val Lys Asp Val Phe  Ala Phe Gly 
    1175                 1180                 1185             


Gly Ala  Val Glu Asn Pro Glu  Tyr Leu Thr Pro Gln  Gly Gly Ala 
    1190                 1195                 1200             


Ala Pro  Gln Pro His Pro Pro  Pro Ala Phe Ser Pro  Ala Phe Asp 
    1205                 1210                 1215             


Asn Leu  Tyr Tyr Trp Asp Gln  Asp Pro Pro Glu Arg  Gly Ala Pro 
    1220                 1225                 1230             


Pro Ser  Thr Phe Lys Gly Thr  Pro Thr Ala Glu Asn  Pro Glu Tyr 
    1235                 1240                 1245             


Leu Gly  Leu Asp Val Pro Val  
    1250                 1255 


<210>  3
<211>  3780
<212>  DNA
<213>  Canine

<300>
<308>  NM_001003217.1
<309>  2014-02-23
<313>  (1)..(3780)

<400>  3
atggagctgg cggcctggtg ccgctggggg ctccttctcg ccctcctgcc ctccggagcc       60

gcgggcaccc aagtgtgcac cggcacagac atgaagctcc ggctcccggc cagtcccgag      120

acccacctgg atatgctccg ccacctgtac cagggctgtc aagtggtaca ggggaacctg      180

gagctcactt acctgcctgc caatgccagc ctgtccttcc tgcaggatat ccaggaggtg      240

cagggctatg tgctcattgc tcacagccaa gtgaggcaga tcccactgca gaggctacga      300

attgtgcgag gcacccagct ctttgaggac aactacgccc tggccgtgct ggacaatgga      360

gacccgctgg agggtggcat ccctgcacca ggggcggccc aaggagggct gcgggagctg      420

cagcttcgaa gcctcacaga gatcctgaag ggaggggtct tgattcagcg gagcccgcag      480

ctctgccacc aggacacgat tttatggaag gacgtcttcc ataagaacaa ccagctggcc      540

ctcacgctga tagacaccaa ccgcttttcg gcctgcccgc cctgttctcc agcttgtaaa      600

gacgcccact gctggggggc cagctccggg gactgtcaga gcttgacgcg gactgtctgt      660

gccgggggct gtgcccgctg caagggccca caacccaccg actgctgcca cgagcagtgt      720

gctgctggct gcacgggccc caagcactct gactgcctgg cctgccttca cttcaaccac      780

agtggcatct gtgagctgca ctgcccagcc ctggtcacct acaacacgga caccttcgaa      840

tccatgccca accctgaggg ccgatatacc ttcggggcca gctgtgtgac ctcctgtccc      900

tacaactacc tgtctacgga tgtgggatcc tgcaccctgg tctgtcccct gaacaaccaa      960

gaggtgacgg ctgaggatgg gacacagcgg tgcgagaaat gcagcaagcc ctgtgcccga     1020

gtgtgctacg gtctgggcat ggagcacctg cgagaggtga gagcggtcac cagtgcgaac     1080

atccaggagt ttgccggctg caagaagatc tttggaagcc tggcattttt gccagagagc     1140

tttgatgggg acccagcctc caacactgcc cccctacagc ctgagcagct cagagtgttt     1200

gaggctctgg aggagatcac aggttacctg tacatctcag cgtggccaga cagcctgcct     1260

aacctcagtg tcttccagaa cctgcgagta atccggggac gagttctgca tgatggtgcc     1320

tactcgctga ccctgcaagg gctgggcatc agctggctgg ggctgcgctc gctgcgggaa     1380

ctgggcagtg ggctggccct catccaccgc aacgcccgcc tttgcttcgt gcacacggtg     1440

ccctgggacc agctcttccg gaacccccac caggccctgc tccatagtgc caaccggcca     1500

gaggaggagt gcgtgggcga gggcctggcc tgctacccct gtgcccatgg gcactgctgg     1560

ggtccagggc ccacccagtg cgtcaactgc agccaattcc tccggggcca ggagtgcgtg     1620

gaggaatgcc gagtactgca ggggctgccc cgagagtatg tgaaggacag gtactgtcta     1680

ccgtgccact cagagtgtca gccccagaat ggctcagtga cctgtttcgg atcggaggct     1740

gaccagtgtg tggcctgcgc ccactacaag gaccctccct tctgtgtggc tcgctgcccc     1800

agtggtgtga aacctgacct gtccttcatg cccatctgga agttcgcaga tgaggagggc     1860

acttgccagc cgtgccccat caactgcacc cactcctgtg cggacctgga cgagaagggc     1920

tgtcccgccg agcagagagc cagccctgtg acatccatca ttgccgctgt ggtgggcatt     1980

ctgctggctg tggtcgtggg gctggtcctc ggcatcctga tcaagcgaag gcggcagaag     2040

atccggaagt acactatgcg gaggctgctg caggaaaccg agctggtgga gccgctgacg     2100

cctagtggag cgatgcccaa ccaggctcag atgcggatcc tgaaagagac agagctgagg     2160

aaggtgaagg tgcttggatc cggagctttt ggcacagtct acaagggcat ctggatccct     2220

gatggggaaa atgtgaaaat cccagtggcc atcaaagtgt tgagggaaaa cacatctccc     2280

aaagccaaca aagaaatctt ggacgaagca tatgtgatgg ctggagtggg ctccccgtat     2340

gtgtcccgcc tcctgggcat ctgcctgaca tccacggtgc agctggtgac acagcttatg     2400

ccctacggct gcctcttaga ccatgtccga gaacaccgtg ggcgcctggg ctcccaggac     2460

ttgctgaact ggtgtgtgca gattgccaag gggatgagct acttggagga tgtccggctg     2520

gtgcacaggg acctggctgc ccggaatgtg ctggtcaaga gtcccaacca tgtcaagatt     2580

acagatttcg ggctggctcg gttgctggac atcgacgaga cagagtacca tgcggatggg     2640

ggcaaggtgc ccatcaagtg gatggcgctg gagtccattc ctccgcggcg gttcacccac     2700

cagagtgatg tgtggagcta tggtgtgact gtgtgggaac tgatgacttt tggggccaaa     2760

ccttatgatg ggatcccagc ccgggagatc cctgacctgc tggagaaggg ggaacggctg     2820

ccccagcccc ccatctgcac cattgatgtc tacatgatca tggtcaagtg ctggatgata     2880

gactctgaat gccgaccccg gttccgggag ttggtggccg aattctcacg tatggccagg     2940

gacccccagc gctttgtggt cattcagaat gaagacttgg gccccgccag ccccttggac     3000

agcaccttct accgttcact actggaagat gatgacatgg gggacctggt ggatgctgag     3060

gagtacctgg taccccagca gggtttcttc tgcccagaac ctaccccagg ggctgggggc     3120

actgcccacc gacggcaccg cagctcatcc accaggaatg gcggtggtga gctgactcta     3180

ggactggagc cctccgagga ggagcccccc aagtctccac tggcaccctc agagggcgct     3240

ggctctgacg tgtttgatgg tgacttggga atgggggcag ccaaggggct gcagagcctt     3300

ccctcacagg accccagccc tctccagcgg tacagtgagg accctacggt acccttgccc     3360

cctgagactg atggtaaggt tgcccccctg acctgcagcc cccagcctga atatgtgaac     3420

cagccagaag tttggccgca gccccccctt gccctagaag gccctttgcc tccttcccga     3480

ccggctggtg ccactctgga aaggcccaag actctgtccc ccaagactct ctcccctggc     3540

aagaatgggg ttgtcaaaga cgtttttgcc tttgggagtg ctgtggagaa tccggagtac     3600

ctggcacccc ggggcagagc tgcccctcag ccccaccctc ctccagcctt cagcccagcc     3660

tttgacaacc tgtattactg ggaccaggat ccatcagagc ggggctctcc acccagcacc     3720

tttgaaggga cccctacagc agagaacccg gagtacctgg ggctggacgt gccagtgtga     3780


<210>  4
<211>  1259
<212>  PRT
<213>  Canine

<300>
<308>  NP_001003217.1
<309>  2014-02-23
<313>  (1)..(1259)

<400>  4

Met Glu Leu Ala Ala Trp Cys Arg Trp Gly Leu Leu Leu Ala Leu Leu 
1               5                   10                  15      


Pro Ser Gly Ala Ala Gly Thr Gln Val Cys Thr Gly Thr Asp Met Lys 
            20                  25                  30          


Leu Arg Leu Pro Ala Ser Pro Glu Thr His Leu Asp Met Leu Arg His 
        35                  40                  45              


Leu Tyr Gln Gly Cys Gln Val Val Gln Gly Asn Leu Glu Leu Thr Tyr 
    50                  55                  60                  


Leu Pro Ala Asn Ala Ser Leu Ser Phe Leu Gln Asp Ile Gln Glu Val 
65                  70                  75                  80  


Gln Gly Tyr Val Leu Ile Ala His Ser Gln Val Arg Gln Ile Pro Leu 
                85                  90                  95      


Gln Arg Leu Arg Ile Val Arg Gly Thr Gln Leu Phe Glu Asp Asn Tyr 
            100                 105                 110         


Ala Leu Ala Val Leu Asp Asn Gly Asp Pro Leu Glu Gly Gly Ile Pro 
        115                 120                 125             


Ala Pro Gly Ala Ala Gln Gly Gly Leu Arg Glu Leu Gln Leu Arg Ser 
    130                 135                 140                 


Leu Thr Glu Ile Leu Lys Gly Gly Val Leu Ile Gln Arg Ser Pro Gln 
145                 150                 155                 160 


Leu Cys His Gln Asp Thr Ile Leu Trp Lys Asp Val Phe His Lys Asn 
                165                 170                 175     


Asn Gln Leu Ala Leu Thr Leu Ile Asp Thr Asn Arg Phe Ser Ala Cys 
            180                 185                 190         


Pro Pro Cys Ser Pro Ala Cys Lys Asp Ala His Cys Trp Gly Ala Ser 
        195                 200                 205             


Ser Gly Asp Cys Gln Ser Leu Thr Arg Thr Val Cys Ala Gly Gly Cys 
    210                 215                 220                 


Ala Arg Cys Lys Gly Pro Gln Pro Thr Asp Cys Cys His Glu Gln Cys 
225                 230                 235                 240 


Ala Ala Gly Cys Thr Gly Pro Lys His Ser Asp Cys Leu Ala Cys Leu 
                245                 250                 255     


His Phe Asn His Ser Gly Ile Cys Glu Leu His Cys Pro Ala Leu Val 
            260                 265                 270         


Thr Tyr Asn Thr Asp Thr Phe Glu Ser Met Pro Asn Pro Glu Gly Arg 
        275                 280                 285             


Tyr Thr Phe Gly Ala Ser Cys Val Thr Ser Cys Pro Tyr Asn Tyr Leu 
    290                 295                 300                 


Ser Thr Asp Val Gly Ser Cys Thr Leu Val Cys Pro Leu Asn Asn Gln 
305                 310                 315                 320 


Glu Val Thr Ala Glu Asp Gly Thr Gln Arg Cys Glu Lys Cys Ser Lys 
                325                 330                 335     


Pro Cys Ala Arg Val Cys Tyr Gly Leu Gly Met Glu His Leu Arg Glu 
            340                 345                 350         


Val Arg Ala Val Thr Ser Ala Asn Ile Gln Glu Phe Ala Gly Cys Lys 
        355                 360                 365             


Lys Ile Phe Gly Ser Leu Ala Phe Leu Pro Glu Ser Phe Asp Gly Asp 
    370                 375                 380                 


Pro Ala Ser Asn Thr Ala Pro Leu Gln Pro Glu Gln Leu Arg Val Phe 
385                 390                 395                 400 


Glu Ala Leu Glu Glu Ile Thr Gly Tyr Leu Tyr Ile Ser Ala Trp Pro 
                405                 410                 415     


Asp Ser Leu Pro Asn Leu Ser Val Phe Gln Asn Leu Arg Val Ile Arg 
            420                 425                 430         


Gly Arg Val Leu His Asp Gly Ala Tyr Ser Leu Thr Leu Gln Gly Leu 
        435                 440                 445             


Gly Ile Ser Trp Leu Gly Leu Arg Ser Leu Arg Glu Leu Gly Ser Gly 
    450                 455                 460                 


Leu Ala Leu Ile His Arg Asn Ala Arg Leu Cys Phe Val His Thr Val 
465                 470                 475                 480 


Pro Trp Asp Gln Leu Phe Arg Asn Pro His Gln Ala Leu Leu His Ser 
                485                 490                 495     


Ala Asn Arg Pro Glu Glu Glu Cys Val Gly Glu Gly Leu Ala Cys Tyr 
            500                 505                 510         


Pro Cys Ala His Gly His Cys Trp Gly Pro Gly Pro Thr Gln Cys Val 
        515                 520                 525             


Asn Cys Ser Gln Phe Leu Arg Gly Gln Glu Cys Val Glu Glu Cys Arg 
    530                 535                 540                 


Val Leu Gln Gly Leu Pro Arg Glu Tyr Val Lys Asp Arg Tyr Cys Leu 
545                 550                 555                 560 


Pro Cys His Ser Glu Cys Gln Pro Gln Asn Gly Ser Val Thr Cys Phe 
                565                 570                 575     


Gly Ser Glu Ala Asp Gln Cys Val Ala Cys Ala His Tyr Lys Asp Pro 
            580                 585                 590         


Pro Phe Cys Val Ala Arg Cys Pro Ser Gly Val Lys Pro Asp Leu Ser 
        595                 600                 605             


Phe Met Pro Ile Trp Lys Phe Ala Asp Glu Glu Gly Thr Cys Gln Pro 
    610                 615                 620                 


Cys Pro Ile Asn Cys Thr His Ser Cys Ala Asp Leu Asp Glu Lys Gly 
625                 630                 635                 640 


Cys Pro Ala Glu Gln Arg Ala Ser Pro Val Thr Ser Ile Ile Ala Ala 
                645                 650                 655     


Val Val Gly Ile Leu Leu Ala Val Val Val Gly Leu Val Leu Gly Ile 
            660                 665                 670         


Leu Ile Lys Arg Arg Arg Gln Lys Ile Arg Lys Tyr Thr Met Arg Arg 
        675                 680                 685             


Leu Leu Gln Glu Thr Glu Leu Val Glu Pro Leu Thr Pro Ser Gly Ala 
    690                 695                 700                 


Met Pro Asn Gln Ala Gln Met Arg Ile Leu Lys Glu Thr Glu Leu Arg 
705                 710                 715                 720 


Lys Val Lys Val Leu Gly Ser Gly Ala Phe Gly Thr Val Tyr Lys Gly 
                725                 730                 735     


Ile Trp Ile Pro Asp Gly Glu Asn Val Lys Ile Pro Val Ala Ile Lys 
            740                 745                 750         


Val Leu Arg Glu Asn Thr Ser Pro Lys Ala Asn Lys Glu Ile Leu Asp 
        755                 760                 765             


Glu Ala Tyr Val Met Ala Gly Val Gly Ser Pro Tyr Val Ser Arg Leu 
    770                 775                 780                 


Leu Gly Ile Cys Leu Thr Ser Thr Val Gln Leu Val Thr Gln Leu Met 
785                 790                 795                 800 


Pro Tyr Gly Cys Leu Leu Asp His Val Arg Glu His Arg Gly Arg Leu 
                805                 810                 815     


Gly Ser Gln Asp Leu Leu Asn Trp Cys Val Gln Ile Ala Lys Gly Met 
            820                 825                 830         


Ser Tyr Leu Glu Asp Val Arg Leu Val His Arg Asp Leu Ala Ala Arg 
        835                 840                 845             


Asn Val Leu Val Lys Ser Pro Asn His Val Lys Ile Thr Asp Phe Gly 
    850                 855                 860                 


Leu Ala Arg Leu Leu Asp Ile Asp Glu Thr Glu Tyr His Ala Asp Gly 
865                 870                 875                 880 


Gly Lys Val Pro Ile Lys Trp Met Ala Leu Glu Ser Ile Pro Pro Arg 
                885                 890                 895     


Arg Phe Thr His Gln Ser Asp Val Trp Ser Tyr Gly Val Thr Val Trp 
            900                 905                 910         


Glu Leu Met Thr Phe Gly Ala Lys Pro Tyr Asp Gly Ile Pro Ala Arg 
        915                 920                 925             


Glu Ile Pro Asp Leu Leu Glu Lys Gly Glu Arg Leu Pro Gln Pro Pro 
    930                 935                 940                 


Ile Cys Thr Ile Asp Val Tyr Met Ile Met Val Lys Cys Trp Met Ile 
945                 950                 955                 960 


Asp Ser Glu Cys Arg Pro Arg Phe Arg Glu Leu Val Ala Glu Phe Ser 
                965                 970                 975     


Arg Met Ala Arg Asp Pro Gln Arg Phe Val Val Ile Gln Asn Glu Asp 
            980                 985                 990         


Leu Gly Pro Ala Ser Pro Leu Asp  Ser Thr Phe Tyr Arg  Ser Leu Leu 
        995                 1000                 1005             


Glu Asp  Asp Asp Met Gly Asp  Leu Val Asp Ala Glu  Glu Tyr Leu 
    1010                 1015                 1020             


Val Pro  Gln Gln Gly Phe Phe  Cys Pro Glu Pro Thr  Pro Gly Ala 
    1025                 1030                 1035             


Gly Gly  Thr Ala His Arg Arg  His Arg Ser Ser Ser  Thr Arg Asn 
    1040                 1045                 1050             


Gly Gly  Gly Glu Leu Thr Leu  Gly Leu Glu Pro Ser  Glu Glu Glu 
    1055                 1060                 1065             


Pro Pro  Lys Ser Pro Leu Ala  Pro Ser Glu Gly Ala  Gly Ser Asp 
    1070                 1075                 1080             


Val Phe  Asp Gly Asp Leu Gly  Met Gly Ala Ala Lys  Gly Leu Gln 
    1085                 1090                 1095             


Ser Leu  Pro Ser Gln Asp Pro  Ser Pro Leu Gln Arg  Tyr Ser Glu 
    1100                 1105                 1110             


Asp Pro  Thr Val Pro Leu Pro  Pro Glu Thr Asp Gly  Lys Val Ala 
    1115                 1120                 1125             


Pro Leu  Thr Cys Ser Pro Gln  Pro Glu Tyr Val Asn  Gln Pro Glu 
    1130                 1135                 1140             


Val Trp  Pro Gln Pro Pro Leu  Ala Leu Glu Gly Pro  Leu Pro Pro 
    1145                 1150                 1155             


Ser Arg  Pro Ala Gly Ala Thr  Leu Glu Arg Pro Lys  Thr Leu Ser 
    1160                 1165                 1170             


Pro Lys  Thr Leu Ser Pro Gly  Lys Asn Gly Val Val  Lys Asp Val 
    1175                 1180                 1185             


Phe Ala  Phe Gly Ser Ala Val  Glu Asn Pro Glu Tyr  Leu Ala Pro 
    1190                 1195                 1200             


Arg Gly  Arg Ala Ala Pro Gln  Pro His Pro Pro Pro  Ala Phe Ser 
    1205                 1210                 1215             


Pro Ala  Phe Asp Asn Leu Tyr  Tyr Trp Asp Gln Asp  Pro Ser Glu 
    1220                 1225                 1230             


Arg Gly  Ser Pro Pro Ser Thr  Phe Glu Gly Thr Pro  Thr Ala Glu 
    1235                 1240                 1245             


Asn Pro  Glu Tyr Leu Gly Leu  Asp Val Pro Val 
    1250                 1255                 


<210>  5
<211>  1311
<212>  DNA
<213>  Homo sapiens

<300>
<308>  NM_000073.2
<309>  2014-05-03
<313>  (1)..(1311)

<400>  5
agtctagctg ctgcacaggc tggctggctg gctggctgct aagggctgct ccacgctttt       60

gccggaggac agagactgac atggaacagg ggaagggcct ggctgtcctc atcctggcta      120

tcattcttct tcaaggtact ttggcccagt caatcaaagg aaaccacttg gttaaggtgt      180

atgactatca agaagatggt tcggtacttc tgacttgtga tgcagaagcc aaaaatatca      240

catggtttaa agatgggaag atgatcggct tcctaactga agataaaaaa aaatggaatc      300

tgggaagtaa tgccaaggac cctcgaggga tgtatcagtg taaaggatca cagaacaagt      360

caaaaccact ccaagtgtat tacagaatgt gtcagaactg cattgaacta aatgcagcca      420

ccatatctgg ctttctcttt gctgaaatcg tcagcatttt cgtccttgct gttggggtct      480

acttcattgc tggacaggat ggagttcgcc agtcgagagc ttcagacaag cagactctgt      540

tgcccaatga ccagctctac cagcccctca aggatcgaga agatgaccag tacagccacc      600

ttcaaggaaa ccagttgagg aggaattgaa ctcaggactc agagtagtcc aggtgttctc      660

ctcctattca gttcccagaa tcaaagcaat gcattttgga aagctcctag cagagagact      720

ttcagcccta aatctagact caaggttccc agagatgaca aatggagaag aaaggccatc      780

agagcaaatt tgggggtttc tcaaataaaa taaaaataaa aacaaatact gtgtttcaga      840

agcgccacct attggggaaa attgtaaaag aaaaatgaaa agatcaaata accccctgga      900

tttgaatata attttttgtg ttgtaatttt tatttcgttt ttgtataggt tataattcac      960

atggctcaaa tattcagtga aagctctccc tccaccgcca tcccctgcta cccagtgacc     1020

ctgttgccct cttcagagac aaattagttt ctcttttttt tttttttttt tttttttttg     1080

agacagtctg gctctgtcac ccaggctgaa atgcagtggc accatctcgg ctcactgcaa     1140

cctctgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgggcag ctgggattac     1200

aggcacacac taccacacct ggctaatttt tgtattttta gtagagacag ggttttgctc     1260

tgttggccaa gctggtctcg aactcctgac ctcaagtgat ccgcccgcct c              1311


<210>  6
<211>  182
<212>  PRT
<213>  Homo sapiens

<300>
<308>  NP_000064.1
<309>  2014-05-03
<313>  (1)..(182)

<400>  6

Met Glu Gln Gly Lys Gly Leu Ala Val Leu Ile Leu Ala Ile Ile Leu 
1               5                   10                  15      


Leu Gln Gly Thr Leu Ala Gln Ser Ile Lys Gly Asn His Leu Val Lys 
            20                  25                  30          


Val Tyr Asp Tyr Gln Glu Asp Gly Ser Val Leu Leu Thr Cys Asp Ala 
        35                  40                  45              


Glu Ala Lys Asn Ile Thr Trp Phe Lys Asp Gly Lys Met Ile Gly Phe 
    50                  55                  60                  


Leu Thr Glu Asp Lys Lys Lys Trp Asn Leu Gly Ser Asn Ala Lys Asp 
65                  70                  75                  80  


Pro Arg Gly Met Tyr Gln Cys Lys Gly Ser Gln Asn Lys Ser Lys Pro 
                85                  90                  95      


Leu Gln Val Tyr Tyr Arg Met Cys Gln Asn Cys Ile Glu Leu Asn Ala 
            100                 105                 110         


Ala Thr Ile Ser Gly Phe Leu Phe Ala Glu Ile Val Ser Ile Phe Val 
        115                 120                 125             


Leu Ala Val Gly Val Tyr Phe Ile Ala Gly Gln Asp Gly Val Arg Gln 
    130                 135                 140                 


Ser Arg Ala Ser Asp Lys Gln Thr Leu Leu Pro Asn Asp Gln Leu Tyr 
145                 150                 155                 160 


Gln Pro Leu Lys Asp Arg Glu Asp Asp Gln Tyr Ser His Leu Gln Gly 
                165                 170                 175     


Asn Gln Leu Arg Arg Asn 
            180         


<210>  7
<211>  771
<212>  DNA
<213>  Homo sapiens

<300>
<308>  NM_000732.4
<309>  2014-05-03
<313>  (1)..(771)

<400>  7
agagaagcag acatcttcta gttcctcccc cactctcctc tttccggtac ctgtgagtca       60

gctaggggag ggcagctctc acccaggctg atagttcggt gacctggctt tatctactgg      120

atgagttccg ctgggagatg gaacatagca cgtttctctc tggcctggta ctggctaccc      180

ttctctcgca agtgagcccc ttcaagatac ctatagagga acttgaggac agagtgtttg      240

tgaattgcaa taccagcatc acatgggtag agggaacggt gggaacactg ctctcagaca      300

ttacaagact ggacctggga aaacgcatcc tggacccacg aggaatatat aggtgtaatg      360

ggacagatat atacaaggac aaagaatcta ccgtgcaagt tcattatcga atgtgccaga      420

gctgtgtgga gctggatcca gccaccgtgg ctggcatcat tgtcactgat gtcattgcca      480

ctctgctcct tgctttggga gtcttctgct ttgctggaca tgagactgga aggctgtctg      540

gggctgccga cacacaagct ctgttgagga atgaccaggt ctatcagccc ctccgagatc      600

gagatgatgc tcagtacagc caccttggag gaaactgggc tcggaacaag tgaacctgag      660

actggtggct tctagaagca gccattacca actgtacctt cccttcttgc tcagccaata      720

aatatatcct ctttcactca gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a               771


<210>  8
<211>  171
<212>  PRT
<213>  Homo sapiens

<300>
<308>  NP_000723.1
<309>  2014-05-03
<313>  (1)..(171)

<400>  8

Met Glu His Ser Thr Phe Leu Ser Gly Leu Val Leu Ala Thr Leu Leu 
1               5                   10                  15      


Ser Gln Val Ser Pro Phe Lys Ile Pro Ile Glu Glu Leu Glu Asp Arg 
            20                  25                  30          


Val Phe Val Asn Cys Asn Thr Ser Ile Thr Trp Val Glu Gly Thr Val 
        35                  40                  45              


Gly Thr Leu Leu Ser Asp Ile Thr Arg Leu Asp Leu Gly Lys Arg Ile 
    50                  55                  60                  


Leu Asp Pro Arg Gly Ile Tyr Arg Cys Asn Gly Thr Asp Ile Tyr Lys 
65                  70                  75                  80  


Asp Lys Glu Ser Thr Val Gln Val His Tyr Arg Met Cys Gln Ser Cys 
                85                  90                  95      


Val Glu Leu Asp Pro Ala Thr Val Ala Gly Ile Ile Val Thr Asp Val 
            100                 105                 110         


Ile Ala Thr Leu Leu Leu Ala Leu Gly Val Phe Cys Phe Ala Gly His 
        115                 120                 125             


Glu Thr Gly Arg Leu Ser Gly Ala Ala Asp Thr Gln Ala Leu Leu Arg 
    130                 135                 140                 


Asn Asp Gln Val Tyr Gln Pro Leu Arg Asp Arg Asp Asp Ala Gln Tyr 
145                 150                 155                 160 


Ser His Leu Gly Gly Asn Trp Ala Arg Asn Lys 
                165                 170     


<210>  9
<211>  1534
<212>  DNA
<213>  Homo sapiens

<300>
<308>  NM_000733.3
<309>  2014-05-03
<313>  (1)..(1534)

<400>  9
tattgtcaga gtcctcttgt ttggccttct aggaaggctg tgggacccag ctttcttcaa       60

ccagtccagg tggaggcctc tgccttgaac gtttccaagt gaggtaaaac ccgcaggccc      120

agaggcctct ctacttcctg tgtggggttc agaaaccctc ctcccctccc agcctcaggt      180

gcctgcttca gaaaatgaag tagtaagtct gctggcctcc gccatcttag taaagtaaca      240

gtcccatgaa acaaagatgc agtcgggcac tcactggaga gttctgggcc tctgcctctt      300

atcagttggc gtttgggggc aagatggtaa tgaagaaatg ggtggtatta cacagacacc      360

atataaagtc tccatctctg gaaccacagt aatattgaca tgccctcagt atcctggatc      420

tgaaatacta tggcaacaca atgataaaaa cataggcggt gatgaggatg ataaaaacat      480

aggcagtgat gaggatcacc tgtcactgaa ggaattttca gaattggagc aaagtggtta      540

ttatgtctgc taccccagag gaagcaaacc agaagatgcg aacttttatc tctacctgag      600

ggcaagagtg tgtgagaact gcatggagat ggatgtgatg tcggtggcca caattgtcat      660

agtggacatc tgcatcactg ggggcttgct gctgctggtt tactactgga gcaagaatag      720

aaaggccaag gccaagcctg tgacacgagg agcgggtgct ggcggcaggc aaaggggaca      780

aaacaaggag aggccaccac ctgttcccaa cccagactat gagcccatcc ggaaaggcca      840

gcgggacctg tattctggcc tgaatcagag acgcatctga ccctctggag aacactgcct      900

cccgctggcc caggtctcct ctccagtccc cctgcgactc cctgtttcct gggctagtct      960

tggaccccac gagagagaat cgttcctcag cctcatggtg aactcgcgcc ctccagcctg     1020

atcccccgct ccctcctccc tgccttctct gctggtaccc agtcctaaaa tattgctgct     1080

tcctcttcct ttgaagcatc atcagtagtc acaccctcac agctggcctg ccctcttgcc     1140

aggatattta tttgtgctat tcactccctt ccctttggat gtaacttctc cgttcagttc     1200

cctccttttc ttgcatgtaa gttgtccccc atcccaaagt attccatcta cttttctatc     1260

gccgtcccct tttgcagccc tctctgggga tggactgggt aaatgttgac agaggccctg     1320

ccccgttcac agatcctggc cctgagccag ccctgtgctc ctccctcccc caacactccc     1380

taccaacccc ctaatcccct actccctcca ccccccctcc actgtaggcc actggatggt     1440

catttgcatc tccgtaaatg tgctctgctc ctcagctgag agagaaaaaa ataaactgta     1500

tttggctgca agaaaaaaaa aaaaaaaaaa aaaa                                 1534


<210>  10
<211>  207
<212>  PRT
<213>  Homo sapiens

<300>
<308>  NP_000724.1
<309>  2014-05-03
<313>  (1)..(207)

<400>  10

Met Gln Ser Gly Thr His Trp Arg Val Leu Gly Leu Cys Leu Leu Ser 
1               5                   10                  15      


Val Gly Val Trp Gly Gln Asp Gly Asn Glu Glu Met Gly Gly Ile Thr 
            20                  25                  30          


Gln Thr Pro Tyr Lys Val Ser Ile Ser Gly Thr Thr Val Ile Leu Thr 
        35                  40                  45              


Cys Pro Gln Tyr Pro Gly Ser Glu Ile Leu Trp Gln His Asn Asp Lys 
    50                  55                  60                  


Asn Ile Gly Gly Asp Glu Asp Asp Lys Asn Ile Gly Ser Asp Glu Asp 
65                  70                  75                  80  


His Leu Ser Leu Lys Glu Phe Ser Glu Leu Glu Gln Ser Gly Tyr Tyr 
                85                  90                  95      


Val Cys Tyr Pro Arg Gly Ser Lys Pro Glu Asp Ala Asn Phe Tyr Leu 
            100                 105                 110         


Tyr Leu Arg Ala Arg Val Cys Glu Asn Cys Met Glu Met Asp Val Met 
        115                 120                 125             


Ser Val Ala Thr Ile Val Ile Val Asp Ile Cys Ile Thr Gly Gly Leu 
    130                 135                 140                 


Leu Leu Leu Val Tyr Tyr Trp Ser Lys Asn Arg Lys Ala Lys Ala Lys 
145                 150                 155                 160 


Pro Val Thr Arg Gly Ala Gly Ala Gly Gly Arg Gln Arg Gly Gln Asn 
                165                 170                 175     


Lys Glu Arg Pro Pro Pro Val Pro Asn Pro Asp Tyr Glu Pro Ile Arg 
            180                 185                 190         


Lys Gly Gln Arg Asp Leu Tyr Ser Gly Leu Asn Gln Arg Arg Ile 
        195                 200                 205         


<210>  11
<211>  1436
<212>  DNA
<213>  Canine

<300>
<308>  NM_001003379.1
<309>  2014-02-22
<313>  (1)..(1436)

<400>  11
ataaacgtta gttactattt ttatcaggac tcctgggacc cctatctcac taatttcctt       60

aaagacagta taatacagca gctcacacag actttcggat tcagaaaaac agttgtgtcg      120

ggccttgggt aaattatgta aggcaagcct cagtttgctc agcggtaaaa cgaggaaagt      180

aataagccac ccgcctccgc cattttgggt agaataaggg tgcatccagt gagagaagga      240

tgcagtcgag gaacctctgg agaattctgg gactctgtct cttatcagtt ggtgcttggg      300

ggcaggacga ggatttcaaa gcttctgatg acttgacaag tatatctcca gagaaacggt      360

ttaaggtctc catctctgga accgaggtag tggtgacatg ccctgatgtt tttggatatg      420

ataatataaa atgggaaaaa aatgataacc ttgtggaagg tgctagtaac agagagctat      480

ctcagaagga gttttcagaa gtggacgaca gtggttatta tgcctgctat gcagattcca      540

taaaggagaa gagctatctc tacctgagag caagagtgtg tgcaaactgc atagaggtga      600

atctgatggc agtggtcaca atcattgtag ctgacatctg ccttactctg gggttgctgc      660

tgatggtgta ttactggagc aagactagaa aggccaatgc caagcctgtg atgagaggaa      720

caggtgccgg cagcaggccc aggggacaaa acaaggagaa gccaccacct gttcccaatc      780

cagactacga gcccatccgg aaaggccagc aggacctgta ttctggcctg aatcagagag      840

gcatctgacg gctcctgagg acacggcctc cccagggccc aggtcttggt gtctccaggt      900

cctgctactc ccagtaccct gggtaaatct tgaaccccag aagagaatta ttcctctgcc      960

ttctggagaa ctaactccca gcctgcagcc ttatccccag caccctccaa cccgccttct     1020

ctgctggcac ttggtcctgc aatatcacct cctcatcatg gccactcacc gcccccccac     1080

cagccagact gccctctggt cggggtattt atttctgtta ccctgacgcc cccaccatca     1140

ccaattcctt cctacccttc agaggtatcc ttgctccctt ccgtacccct ctcccggaca     1200

gaacctgccc ccatccctta ctatcccacc tacctttccg tttttccagc tctctctttg     1260

gtgaccctct gtggggatgg actaggtaac tctggtagag gtcctgcccc atccatgacc     1320

ttggcccaga gccaccctct gccagcaggc ccctggatga tcatttgcat tcttacaaat     1380

gtgctaggct ccttgacagc tagagagaaa ataataaagt gtatttggtt gaaaaa         1436


<210>  12
<211>  202
<212>  PRT
<213>  Canine

<300>
<308>  NP_001003379.1
<309>  2014-02-22
<313>  (1)..(202)

<400>  12

Met Gln Ser Arg Asn Leu Trp Arg Ile Leu Gly Leu Cys Leu Leu Ser 
1               5                   10                  15      


Val Gly Ala Trp Gly Gln Asp Glu Asp Phe Lys Ala Ser Asp Asp Leu 
            20                  25                  30          


Thr Ser Ile Ser Pro Glu Lys Arg Phe Lys Val Ser Ile Ser Gly Thr 
        35                  40                  45              


Glu Val Val Val Thr Cys Pro Asp Val Phe Gly Tyr Asp Asn Ile Lys 
    50                  55                  60                  


Trp Glu Lys Asn Asp Asn Leu Val Glu Gly Ala Ser Asn Arg Glu Leu 
65                  70                  75                  80  


Ser Gln Lys Glu Phe Ser Glu Val Asp Asp Ser Gly Tyr Tyr Ala Cys 
                85                  90                  95      


Tyr Ala Asp Ser Ile Lys Glu Lys Ser Tyr Leu Tyr Leu Arg Ala Arg 
            100                 105                 110         


Val Cys Ala Asn Cys Ile Glu Val Asn Leu Met Ala Val Val Thr Ile 
        115                 120                 125             


Ile Val Ala Asp Ile Cys Leu Thr Leu Gly Leu Leu Leu Met Val Tyr 
    130                 135                 140                 


Tyr Trp Ser Lys Thr Arg Lys Ala Asn Ala Lys Pro Val Met Arg Gly 
145                 150                 155                 160 


Thr Gly Ala Gly Ser Arg Pro Arg Gly Gln Asn Lys Glu Lys Pro Pro 
                165                 170                 175     


Pro Val Pro Asn Pro Asp Tyr Glu Pro Ile Arg Lys Gly Gln Gln Asp 
            180                 185                 190         


Leu Tyr Ser Gly Leu Asn Gln Arg Gly Ile 
        195                 200         


<210>  13
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Peptide linker (G4S)3

<400>  13
ggcggcggag gatctggcgg aggtggaagt gggggaggcg gatct                       45


<210>  14
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker (G4S)3

<400>  14

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  15
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OKT3 VH

<400>  15

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Pro Val Thr Val Ser Ser 
        115                 


<210>  16
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OKT3 VL

<400>  16

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu 
65                  70                  75                  80  


Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr 
                85                  90                  95      


Phe Gly Gln Gly Thr Lys Leu Gln Ile Thr Arg 
            100                 105         


<210>  17
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OKT3 VH C105S

<400>  17

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Ser Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Pro Val Thr Val Ser Ser 
        115                 


<210>  18
<211>  723
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OKT3 scFv C105S

<400>  18
caggtgcagc tggtgcagag tggtggcgga gtggtgcagc ctggcagatc cctgagactg       60

tcttgcaagg ccagcggcta caccttcacc cggtacacca tgcactgggt gcgacaggcc      120

cctggcaagg gcctggaatg gatcggctac atcaacccct cccggggcta caccaactac      180

aaccagaagt tcaaggaccg gttcaccatc agccgggaca actccaagaa caccgccttt      240

ctgcagatgg actccctgcg gcctgaggat accggcgtgt acttttgcgc ccggtactac      300

gacgaccact actctctgga ctactggggc cagggaaccc ctgtgacagt gtctagcgga      360

gggggaggtt caggtggcgg tggatcaggg ggcggaggct ctgatatcca gatgacccag      420

tccccctcca gcctgtctgc ctctgtggga gacagagtga caattacatg ctccgccagc      480

tccagcgtgt cctacatgaa ttggtatcag cagacccctg gcaaggctcc caagcggtgg      540

atctacgaca cctccaagct ggcctccggc gtgccctcca gattttctgg cagcggctcc      600

ggcacagact atacctttac aatcagctcc ctgcagcccg aagatatcgc cacctactac      660

tgccagcagt ggtcctccaa ccccttcacc tttggccagg ggacaaaact gcagatcacc      720

aga                                                                    723


<210>  19
<211>  241
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OKT3 scFv C105S

<400>  19

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Ser Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Pro Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
    130                 135                 140                 


Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser 
145                 150                 155                 160 


Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala 
                165                 170                 175     


Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro 
            180                 185                 190         


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile 
        195                 200                 205             


Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp 
    210                 215                 220                 


Ser Ser Asn Pro Phe Thr Phe Gly Gln Gly Thr Lys Leu Gln Ile Thr 
225                 230                 235                 240 


Arg 
    


<210>  20
<211>  1350
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exemplary heavy chain N297A

<400>  20
gaggtgcagc tggtggaatc tggcggagga ctggtgcagc ctggcggctc tctgagactg       60

tcttgtgccg cctccggctt caacatcaag gacacctaca tccactgggt gcgacaggcc      120

cctggcaagg gactggaatg ggtggccaga atctacccca ccaacggcta caccagatac      180

gccgactctg tgaagggccg gttcaccatc tccgccgaca cctccaagaa caccgcctac      240

ctgcagatga actccctgcg ggccgaggac accgccgtgt actactgtag tagatgggga      300

ggcgacggct tctacgccat ggactattgg ggccagggca ccctcgtgac cgtgtcctct      360

gcttctacca agggcccctc tgtgtttcct ctggccccct ccagcaagtc cacctctggt      420

ggaacagccg ccctgggctg cctcgtgaag gactactttc ccgagcccgt gaccgtgtcc      480

tggaactctg gcgctctgac ctctggcgtg cacaccttcc ctgctgtgct gcagtctagc      540

ggcctgtact ccctgtcctc cgtcgtgaca gtgccctcca gctctctggg cacccagacc      600

tacatctgca acgtgaacca caagccctcc aataccaagg tggacaagcg ggtggaaccc      660

aagtcctgcg acaagaccca cacctgtccc ccttgtcctg cccctgaact gctgggcgga      720

ccttccgtgt tcctgttccc cccaaagccc aaggacaccc tgatgatctc ccggaccccc      780

gaagtgacct gcgtggtggt ggatgtgtcc cacgaggacc ctgaagtgaa gttcaattgg      840

tacgtggacg gcgtggaagt gcacaacgcc aagaccaagc ctagagagga acagtacgcc      900

tccacctacc gggtggtgtc cgtgctgaca gtgctgcacc aggactggct gaacggcaaa      960

gagtacaagt gcaaagtgtc caacaaggcc ctgcctgccc ccatcgaaaa gaccatctcc     1020

aaggccaagg gccagccccg ggaaccccag gtgtacacac tgccccctag cagggacgag     1080

ctgaccaaga accaggtgtc cctgacctgt ctcgtgaaag gcttctaccc ctccgatatc     1140

gccgtggaat gggagtccaa cggccagcct gagaacaact acaagaccac cccccctgtg     1200

ctggactccg acggctcatt cttcctgtac agcaagctga ccgtggacaa gtcccggtgg     1260

cagcagggca acgtgttctc ctgctccgtg atgcacgagg ccctgcacaa ccactacacc     1320

cagaagtccc tgtccctgag ccccggcaaa                                      1350


<210>  21
<211>  1350
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exemplary heavy chain K322A

<400>  21
gaggtgcagc tggtggaatc tggcggagga ctggtgcagc ctggcggctc tctgagactg       60

tcttgtgccg cctccggctt caacatcaag gacacctaca tccactgggt gcgacaggcc      120

cctggcaagg gactggaatg ggtggccaga atctacccca ccaacggcta caccagatac      180

gccgactctg tgaagggccg gttcaccatc tccgccgaca cctccaagaa caccgcctac      240

ctgcagatga actccctgcg ggccgaggac accgccgtgt actactgtag tagatgggga      300

ggcgacggct tctacgccat ggactattgg ggccagggca ccctcgtgac cgtgtcctct      360

gcttctacca agggcccctc tgtgtttcct ctggccccct ccagcaagtc cacctctggt      420

ggaacagccg ccctgggctg cctcgtgaag gactactttc ccgagcccgt gaccgtgtcc      480

tggaactctg gcgctctgac ctctggcgtg cacaccttcc ctgctgtgct gcagtctagc      540

ggcctgtact ccctgtcctc cgtcgtgaca gtgccctcca gctctctggg cacccagacc      600

tacatctgca acgtgaacca caagccctcc aataccaagg tggacaagcg ggtggaaccc      660

aagtcctgcg acaagaccca cacctgtccc ccttgtcctg cccctgaact gctgggcgga      720

ccttccgtgt tcctgttccc cccaaagccc aaggacaccc tgatgatctc ccggaccccc      780

gaagtgacct gcgtggtggt ggatgtgtcc cacgaggacc ctgaagtgaa gttcaattgg      840

tacgtggacg gcgtggaagt gcacaacgcc aagaccaagc ctagagagga acagtacaac      900

tccacctacc gggtggtgtc cgtgctgaca gtgctgcacc aggactggct gaacggcaaa      960

gagtacaagt gcgccgtgtc caacaaggcc ctgcctgccc ccatcgaaaa gaccatctcc     1020

aaggccaagg gccagccccg ggaaccccag gtgtacacac tgccccctag cagggacgag     1080

ctgaccaaga accaggtgtc cctgacctgt ctcgtgaaag gcttctaccc ctccgatatc     1140

gccgtggaat gggagtccaa cggccagcct gagaacaact acaagaccac cccccctgtg     1200

ctggactccg acggctcatt cttcctgtac agcaagctga ccgtggacaa gtcccggtgg     1260

cagcagggca acgtgttctc ctgctccgtg atgcacgagg ccctgcacaa ccactacacc     1320

cagaagtccc tgtccctgag ccccggcaaa                                      1350


<210>  22
<211>  1350
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exemplary heavy chain

<400>  22
gaggtgcagc tggtggaatc tggcggagga ctggtgcagc ctggcggctc tctgagactg       60

tcttgtgccg cctccggctt caacatcaag gacacctaca tccactgggt gcgacaggcc      120

cctggcaagg gactggaatg ggtggccaga atctacccca ccaacggcta caccagatac      180

gccgactctg tgaagggccg gttcaccatc tccgccgaca cctccaagaa caccgcctac      240

ctgcagatga actccctgcg ggccgaggac accgccgtgt actactgtag tagatgggga      300

ggcgacggct tctacgccat ggactattgg ggccagggca ccctcgtgac cgtgtcctct      360

gcttctacca agggcccctc tgtgtttcct ctggccccct ccagcaagtc cacctctggt      420

ggaacagccg ccctgggctg cctcgtgaag gactactttc ccgagcccgt gaccgtgtcc      480

tggaactctg gcgctctgac ctctggcgtg cacaccttcc ctgctgtgct gcagtctagc      540

ggcctgtact ccctgtcctc cgtcgtgaca gtgccctcca gctctctggg cacccagacc      600

tacatctgca acgtgaacca caagccctcc aataccaagg tggacaagcg ggtggaaccc      660

aagtcctgcg acaagaccca cacctgtccc ccttgtcctg cccctgaact gctgggcgga      720

ccttccgtgt tcctgttccc cccaaagccc aaggacaccc tgatgatctc ccggaccccc      780

gaagtgacct gcgtggtggt ggatgtgtcc cacgaggacc ctgaagtgaa gttcaattgg      840

tacgtggacg gcgtggaagt gcacaacgcc aagaccaagc ctagagagga acagtacaac      900

tccacctacc gggtggtgtc cgtgctgaca gtgctgcacc aggactggct gaacggcaaa      960

gagtacaagt gcaaagtgtc caacaaggcc ctgcctgccc ccatcgaaaa gaccatctcc     1020

aaggccaagg gccagccccg ggaaccccag gtgtacacac tgccccctag cagggacgag     1080

ctgaccaaga accaggtgtc cctgacctgt ctcgtgaaag gcttctaccc ctccgatatc     1140

gccgtggaat gggagtccaa cggccagcct gagaacaact acaagaccac cccccctgtg     1200

ctggactccg acggctcatt cttcctgtac agcaagctga ccgtggacaa gtcccggtgg     1260

cagcagggca acgtgttctc ctgctccgtg atgcacgagg ccctgcacaa ccactacacc     1320

cagaagtccc tgtccctgag ccccggcaaa                                      1350


<210>  23
<211>  450
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  exemplary heavy chain

<400>  23

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr 
            20                  25                  30          


Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val 
        115                 120                 125             


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
    130                 135                 140                 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
145                 150                 155                 160 


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
                165                 170                 175     


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
            180                 185                 190         


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
        195                 200                 205             


Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp 
    210                 215                 220                 


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
225                 230                 235                 240 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
                245                 250                 255     


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
            260                 265                 270         


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
        275                 280                 285             


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
    290                 295                 300                 


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
305                 310                 315                 320 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
                325                 330                 335     


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
            340                 345                 350         


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
        355                 360                 365             


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
    370                 375                 380                 


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
385                 390                 395                 400 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
                405                 410                 415     


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
            420                 425                 430         


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
        435                 440                 445             


Gly Lys 
    450 


<210>  24
<211>  642
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HER2-BsAb light chain

<400>  24
gacatccaga tgacccagag cccttccagc ctgtccgcct ctgtgggcga cagagtgacc       60

atcacctgtc gggcctccca ggacgtgaac accgccgtgg cttggtatca gcagaagccc      120

ggcaaggccc ccaagctgct gatctactcc gcctccttcc tgtactccgg cgtgccctcc      180

agattctccg gcagcagatc tggcaccgac tttaccctga ccatctccag cctgcagccc      240

gaggacttcg ccacctacta ctgccagcag cactacacca ccccccccac ctttggccag      300

ggcaccaagg tggaaatcaa gcggaccgtg gccgctccct ccgtgttcat cttcccacct      360

tccgacgagc agctgaagtc cggcaccgct tctgtcgtgt gcctgctgaa caacttctac      420

ccccgcgagg ccaaggtgca gtggaaggtg gacaacgccc tgcagtccgg caactcccag      480

gaatccgtga ccgagcagga ctccaaggac agcacctact ccctgtcctc caccctgacc      540

ctgtccaagg ccgactacga gaagcacaag gtgtacgcct gcgaagtgac ccaccagggc      600

ctgtctagcc ccgtgaccaa gtctttcaac cggggcgagt gc                         642


<210>  25
<211>  216
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2-BsAb light chain

<400>  25

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Thr Ser 
    210                 215     


<210>  26
<211>  1350
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HER2-BsAb heavy chain

<400>  26
gaggtgcagc tggtggaatc tggcggagga ctggtgcagc ctggcggctc tctgagactg       60

tcttgtgccg cctccggctt caacatcaag gacacctaca tccactgggt gcgacaggcc      120

cctggcaagg gactggaatg ggtggccaga atctacccca ccaacggcta caccagatac      180

gccgactctg tgaagggccg gttcaccatc tccgccgaca cctccaagaa caccgcctac      240

ctgcagatga actccctgcg ggccgaggac accgccgtgt actactgtag tagatgggga      300

ggcgacggct tctacgccat ggactattgg ggccagggca ccctcgtgac cgtgtcctct      360

gcttctacca agggcccctc tgtgtttcct ctggccccct ccagcaagtc cacctctggt      420

ggaacagccg ccctgggctg cctcgtgaag gactactttc ccgagcccgt gaccgtgtcc      480

tggaactctg gcgctctgac ctctggcgtg cacaccttcc ctgctgtgct gcagtctagc      540

ggcctgtact ccctgtcctc cgtcgtgaca gtgccctcca gctctctggg cacccagacc      600

tacatctgca acgtgaacca caagccctcc aataccaagg tggacaagcg ggtggaaccc      660

aagtcctgcg acaagaccca cacctgtccc ccttgtcctg cccctgaact gctgggcgga      720

ccttccgtgt tcctgttccc cccaaagccc aaggacaccc tgatgatctc ccggaccccc      780

gaagtgacct gcgtggtggt ggatgtgtcc cacgaggacc ctgaagtgaa gttcaattgg      840

tacgtggacg gcgtggaagt gcacaacgcc aagaccaagc ctagagagga acagtacgcc      900

tccacctacc gggtggtgtc cgtgctgaca gtgctgcacc aggactggct gaacggcaaa      960

gagtacaagt gcgccgtgtc caacaaggcc ctgcctgccc ccatcgaaaa gaccatctcc     1020

aaggccaagg gccagccccg ggaaccccag gtgtacacac tgccccctag cagggacgag     1080

ctgaccaaga accaggtgtc cctgacctgt ctcgtgaaag gcttctaccc ctccgatatc     1140

gccgtggaat gggagtccaa cggccagcct gagaacaact acaagaccac cccccctgtg     1200

ctggactccg acggctcatt cttcctgtac agcaagctga ccgtggacaa gtcccggtgg     1260

cagcagggca acgtgttctc ctgctccgtg atgcacgagg ccctgcacaa ccactacacc     1320

cagaagtccc tgtccctgag ccccggcaaa                                      1350


<210>  27
<211>  450
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2-BsAb heavy chain

<400>  27

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr 
            20                  25                  30          


Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val 
        115                 120                 125             


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
    130                 135                 140                 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
145                 150                 155                 160 


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
                165                 170                 175     


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
            180                 185                 190         


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
        195                 200                 205             


Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp 
    210                 215                 220                 


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
225                 230                 235                 240 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
                245                 250                 255     


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
            260                 265                 270         


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
        275                 280                 285             


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr Arg 
    290                 295                 300                 


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
305                 310                 315                 320 


Glu Tyr Lys Cys Ala Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
                325                 330                 335     


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
            340                 345                 350         


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
        355                 360                 365             


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
    370                 375                 380                 


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
385                 390                 395                 400 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
                405                 410                 415     


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
            420                 425                 430         


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
        435                 440                 445             


Gly Lys 
    450 


<210>  28
<211>  1416
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HER2-BsAb light chain fusion polypeptide

<400>  28
gacatccaga tgacccagag cccttccagc ctgtccgcct ctgtgggcga cagagtgacc       60

atcacctgtc gggcctccca ggacgtgaac accgccgtgg cttggtatca gcagaagccc      120

ggcaaggccc ccaagctgct gatctactcc gcctccttcc tgtactccgg cgtgccctcc      180

agattctccg gcagcagatc tggcaccgac tttaccctga ccatctccag cctgcagccc      240

gaggacttcg ccacctacta ctgccagcag cactacacca ccccccccac ctttggccag      300

ggcaccaagg tggaaatcaa gcggaccgtg gccgctccct ccgtgttcat cttcccacct      360

tccgacgagc agctgaagtc cggcaccgct tctgtcgtgt gcctgctgaa caacttctac      420

ccccgcgagg ccaaggtgca gtggaaggtg gacaacgccc tgcagtccgg caactcccag      480

gaatccgtga ccgagcagga ctccaaggac agcacctact ccctgtcctc caccctgacc      540

ctgtccaagg ccgactacga gaagcacaag gtgtacgcct gcgaagtgac ccaccagggc      600

ctgtctagcc ccgtgaccaa gtctttcaac cggggcgagt gcactagtgg cggcggagga      660

tctggcggag gtggaagtgg gggaggcgga tctcaggtgc agctggtgca gagtggtggc      720

ggagtggtgc agcctggcag atccctgaga ctgtcttgca aggccagcgg ctacaccttc      780

acccggtaca ccatgcactg ggtgcgacag gcccctggca agggcctgga atggatcggc      840

tacatcaacc cctcccgggg ctacaccaac tacaaccaga agttcaagga ccggttcacc      900

atcagccggg acaactccaa gaacaccgcc tttctgcaga tggactccct gcggcctgag      960

gataccggcg tgtacttttg cgcccggtac tacgacgacc actactctct ggactactgg     1020

ggccagggaa cccctgtgac agtgtctagc ggagggggag gttcaggtgg cggtggatca     1080

gggggcggag gctctgatat ccagatgacc cagtccccct ccagcctgtc tgcctctgtg     1140

ggagacagag tgacaattac atgctccgcc agctccagcg tgtcctacat gaattggtat     1200

cagcagaccc ctggcaaggc tcccaagcgg tggatctacg acacctccaa gctggcctcc     1260

ggcgtgccct ccagattttc tggcagcggc tccggcacag actatacctt tacaatcagc     1320

tccctgcagc ccgaagatat cgccacctac tactgccagc agtggtcctc caaccccttc     1380

acctttggcc aggggacaaa actgcagatc accaga                               1416


<210>  29
<211>  472
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2-BsAb light chain fusion polypeptide

<400>  29

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Thr Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    210                 215                 220                 


Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Gln Ser Gly Gly 
225                 230                 235                 240 


Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Lys Ala Ser 
                245                 250                 255     


Gly Tyr Thr Phe Thr Arg Tyr Thr Met His Trp Val Arg Gln Ala Pro 
            260                 265                 270         


Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr 
        275                 280                 285             


Thr Asn Tyr Asn Gln Lys Phe Lys Asp Arg Phe Thr Ile Ser Arg Asp 
    290                 295                 300                 


Asn Ser Lys Asn Thr Ala Phe Leu Gln Met Asp Ser Leu Arg Pro Glu 
305                 310                 315                 320 


Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr Tyr Asp Asp His Tyr Ser 
                325                 330                 335     


Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val Thr Val Ser Ser Gly Gly 
            340                 345                 350         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln 
        355                 360                 365             


Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val 
    370                 375                 380                 


Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr 
385                 390                 395                 400 


Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile Tyr Asp Thr Ser 
                405                 410                 415     


Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
            420                 425                 430         


Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala 
        435                 440                 445             


Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr Phe Gly Gln 
    450                 455                 460                 


Gly Thr Lys Leu Gln Ile Thr Arg 
465                 470         



<210>  30
<211>  462
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2-BsAb light chain fusion polypeptide (5aa scFv linker)

<400>  30

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Thr Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    210                 215                 220                 


Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Gln Ser Gly Gly 
225                 230                 235                 240 


Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Lys Ala Ser 
                245                 250                 255     


Gly Tyr Thr Phe Thr Arg Tyr Thr Met His Trp Val Arg Gln Ala Pro 
            260                 265                 270         


Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr 
        275                 280                 285             


Thr Asn Tyr Asn Gln Lys Phe Lys Asp Arg Phe Thr Ile Ser Arg Asp 
    290                 295                 300                 


Asn Ser Lys Asn Thr Ala Phe Leu Gln Met Asp Ser Leu Arg Pro Glu 
305                 310                 315                 320 


Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr Tyr Asp Asp His Tyr Ser 
                325                 330                 335     


Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val Thr Val Ser Ser Gly Gly 
            340                 345                 350         


Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala 
        355                 360                 365             


Ser Val Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val 
    370                 375                 380                 


Ser Tyr Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg 
385                 390                 395                 400 


Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe 
                405                 410                 415     


Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu 
            420                 425                 430         


Gln Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn 
        435                 440                 445             


Pro Phe Thr Phe Gly Gln Gly Thr Lys Leu Gln Ile Thr Arg 
    450                 455                 460         


<210>  31
<211>  467
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2-BsAb light chain fusion polypeptide (10aa scFv linker)

<400>  31

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Thr Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    210                 215                 220                 


Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Gln Ser Gly Gly 
225                 230                 235                 240 


Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Lys Ala Ser 
                245                 250                 255     


Gly Tyr Thr Phe Thr Arg Tyr Thr Met His Trp Val Arg Gln Ala Pro 
            260                 265                 270         


Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr 
        275                 280                 285             


Thr Asn Tyr Asn Gln Lys Phe Lys Asp Arg Phe Thr Ile Ser Arg Asp 
    290                 295                 300                 


Asn Ser Lys Asn Thr Ala Phe Leu Gln Met Asp Ser Leu Arg Pro Glu 
305                 310                 315                 320 


Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr Tyr Asp Asp His Tyr Ser 
                325                 330                 335     


Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val Thr Val Ser Ser Gly Gly 
            340                 345                 350         


Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro 
        355                 360                 365             


Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Ser 
    370                 375                 380                 


Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Thr Pro Gly 
385                 390                 395                 400 


Lys Ala Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly 
                405                 410                 415     


Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Phe 
            420                 425                 430         


Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln 
        435                 440                 445             


Gln Trp Ser Ser Asn Pro Phe Thr Phe Gly Gln Gly Thr Lys Leu Gln 
    450                 455                 460                 


Ile Thr Arg 
465         


<210>  32
<211>  477
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2-BsAb light chain fusion polypeptide (20aa scFv linker)

<400>  32

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Thr Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    210                 215                 220                 


Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Gln Ser Gly Gly 
225                 230                 235                 240 


Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Lys Ala Ser 
                245                 250                 255     


Gly Tyr Thr Phe Thr Arg Tyr Thr Met His Trp Val Arg Gln Ala Pro 
            260                 265                 270         


Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr 
        275                 280                 285             


Thr Asn Tyr Asn Gln Lys Phe Lys Asp Arg Phe Thr Ile Ser Arg Asp 
    290                 295                 300                 


Asn Ser Lys Asn Thr Ala Phe Leu Gln Met Asp Ser Leu Arg Pro Glu 
305                 310                 315                 320 


Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr Tyr Asp Asp His Tyr Ser 
                325                 330                 335     


Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val Thr Val Ser Ser Gly Gly 
            340                 345                 350         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        355                 360                 365             


Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
    370                 375                 380                 


Val Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser 
385                 390                 395                 400 


Tyr Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp 
                405                 410                 415     


Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser 
            420                 425                 430         


Gly Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln 
        435                 440                 445             


Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro 
    450                 455                 460                 


Phe Thr Phe Gly Gln Gly Thr Lys Leu Gln Ile Thr Arg 
465                 470                 475         


<210>  33
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2-BsAb light chain fusion polypeptide (25aa scFv linker)

<400>  33

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Thr Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    210                 215                 220                 


Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Gln Ser Gly Gly 
225                 230                 235                 240 


Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Lys Ala Ser 
                245                 250                 255     


Gly Tyr Thr Phe Thr Arg Tyr Thr Met His Trp Val Arg Gln Ala Pro 
            260                 265                 270         


Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr 
        275                 280                 285             


Thr Asn Tyr Asn Gln Lys Phe Lys Asp Arg Phe Thr Ile Ser Arg Asp 
    290                 295                 300                 


Asn Ser Lys Asn Thr Ala Phe Leu Gln Met Asp Ser Leu Arg Pro Glu 
305                 310                 315                 320 


Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr Tyr Asp Asp His Tyr Ser 
                325                 330                 335     


Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val Thr Val Ser Ser Gly Gly 
            340                 345                 350         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        355                 360                 365             


Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser 
    370                 375                 380                 


Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Ser Ala 
385                 390                 395                 400 


Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys 
                405                 410                 415     


Ala Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val 
            420                 425                 430         


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr 
        435                 440                 445             


Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln 
    450                 455                 460                 


Trp Ser Ser Asn Pro Phe Thr Phe Gly Gln Gly Thr Lys Leu Gln Ile 
465                 470                 475                 480 


Thr Arg 
        


<210>  34
<211>  487
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2-BsAb light chain fusion polypeptide (30aa scFv linker)

<400>  34

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Thr Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    210                 215                 220                 


Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Gln Ser Gly Gly 
225                 230                 235                 240 


Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Lys Ala Ser 
                245                 250                 255     


Gly Tyr Thr Phe Thr Arg Tyr Thr Met His Trp Val Arg Gln Ala Pro 
            260                 265                 270         


Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr 
        275                 280                 285             


Thr Asn Tyr Asn Gln Lys Phe Lys Asp Arg Phe Thr Ile Ser Arg Asp 
    290                 295                 300                 


Asn Ser Lys Asn Thr Ala Phe Leu Gln Met Asp Ser Leu Arg Pro Glu 
305                 310                 315                 320 


Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr Tyr Asp Asp His Tyr Ser 
                325                 330                 335     


Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val Thr Val Ser Ser Gly Gly 
            340                 345                 350         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        355                 360                 365             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met 
    370                 375                 380                 


Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr 
385                 390                 395                 400 


Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln 
                405                 410                 415     


Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys 
            420                 425                 430         


Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 
        435                 440                 445             


Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr 
    450                 455                 460                 


Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr Phe Gly Gln Gly 
465                 470                 475                 480 


Thr Lys Leu Gln Ile Thr Arg 
                485         


<210>  35
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T(G4S)3 Linker

<400>  35

Thr Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   10                  15      


Ser 
    


<210>  36
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G4S Linker

<400>  36

Gly Gly Gly Gly Ser 
1               5   


<210>  37
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (G4S)2 Linker

<400>  37

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  38
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (G4S)3 Linker

<400>  38

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  39
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (G4S)4 Linker

<400>  39

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  40
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (G4S)5 Linker

<400>  40

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            20                  25  


<210>  41
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (G4S)6 Linker

<400>  41

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            20                  25                  30  


<210>  42
<211>  462
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2-BsAb light chain fusion polypeptide 
       (5aa scFv linker) + disulfide mut

<400>  42

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Thr Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    210                 215                 220                 


Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Gln Ser Gly Gly 
225                 230                 235                 240 


Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Lys Ala Ser 
                245                 250                 255     


Gly Tyr Thr Phe Thr Arg Tyr Thr Met His Trp Val Arg Gln Ala Pro 
            260                 265                 270         


Gly Lys Cys Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr 
        275                 280                 285             


Thr Asn Tyr Asn Gln Lys Phe Lys Asp Arg Phe Thr Ile Ser Arg Asp 
    290                 295                 300                 


Asn Ser Lys Asn Thr Ala Phe Leu Gln Met Asp Ser Leu Arg Pro Glu 
305                 310                 315                 320 


Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr Tyr Asp Asp His Tyr Ser 
                325                 330                 335     


Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val Thr Val Ser Ser Gly Gly 
            340                 345                 350         


Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala 
        355                 360                 365             


Ser Val Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val 
    370                 375                 380                 


Ser Tyr Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg 
385                 390                 395                 400 


Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe 
                405                 410                 415     


Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu 
            420                 425                 430         


Gln Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn 
        435                 440                 445             


Pro Phe Thr Phe Gly Cys Gly Thr Lys Leu Gln Ile Thr Arg 
    450                 455                 460         


<210>  43
<211>  467
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2-BsAb light chain fusion polypeptide (10aa scFv linker)
       + disulfide mut

<400>  43

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Thr Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    210                 215                 220                 


Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Gln Ser Gly Gly 
225                 230                 235                 240 


Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Lys Ala Ser 
                245                 250                 255     


Gly Tyr Thr Phe Thr Arg Tyr Thr Met His Trp Val Arg Gln Ala Pro 
            260                 265                 270         


Gly Lys Cys Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr 
        275                 280                 285             


Thr Asn Tyr Asn Gln Lys Phe Lys Asp Arg Phe Thr Ile Ser Arg Asp 
    290                 295                 300                 


Asn Ser Lys Asn Thr Ala Phe Leu Gln Met Asp Ser Leu Arg Pro Glu 
305                 310                 315                 320 


Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr Tyr Asp Asp His Tyr Ser 
                325                 330                 335     


Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val Thr Val Ser Ser Gly Gly 
            340                 345                 350         


Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro 
        355                 360                 365             


Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Ser 
    370                 375                 380                 


Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Thr Pro Gly 
385                 390                 395                 400 


Lys Ala Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly 
                405                 410                 415     


Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Phe 
            420                 425                 430         


Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln 
        435                 440                 445             


Gln Trp Ser Ser Asn Pro Phe Thr Phe Gly Cys Gly Thr Lys Leu Gln 
    450                 455                 460                 


Ile Thr Arg 
465         


<210>  44
<211>  472
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2-BsAb light chain fusion polypeptide (15aa scFv linker)
       + disulfide mut

<400>  44

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Thr Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    210                 215                 220                 


Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Gln Ser Gly Gly 
225                 230                 235                 240 


Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Lys Ala Ser 
                245                 250                 255     


Gly Tyr Thr Phe Thr Arg Tyr Thr Met His Trp Val Arg Gln Ala Pro 
            260                 265                 270         


Gly Lys Cys Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr 
        275                 280                 285             


Thr Asn Tyr Asn Gln Lys Phe Lys Asp Arg Phe Thr Ile Ser Arg Asp 
    290                 295                 300                 


Asn Ser Lys Asn Thr Ala Phe Leu Gln Met Asp Ser Leu Arg Pro Glu 
305                 310                 315                 320 


Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr Tyr Asp Asp His Tyr Ser 
                325                 330                 335     


Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val Thr Val Ser Ser Gly Gly 
            340                 345                 350         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln 
        355                 360                 365             


Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val 
    370                 375                 380                 


Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr 
385                 390                 395                 400 


Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile Tyr Asp Thr Ser 
                405                 410                 415     


Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
            420                 425                 430         


Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala 
        435                 440                 445             


Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr Phe Gly Cys 
    450                 455                 460                 


Gly Thr Lys Leu Gln Ile Thr Arg 
465                 470         


<210>  45
<211>  477
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2-BsAb light chain fusion polypeptide (20aa scFv linker)
       + disulfide mut

<400>  45

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Thr Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    210                 215                 220                 


Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Gln Ser Gly Gly 
225                 230                 235                 240 


Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Lys Ala Ser 
                245                 250                 255     


Gly Tyr Thr Phe Thr Arg Tyr Thr Met His Trp Val Arg Gln Ala Pro 
            260                 265                 270         


Gly Lys Cys Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr 
        275                 280                 285             


Thr Asn Tyr Asn Gln Lys Phe Lys Asp Arg Phe Thr Ile Ser Arg Asp 
    290                 295                 300                 


Asn Ser Lys Asn Thr Ala Phe Leu Gln Met Asp Ser Leu Arg Pro Glu 
305                 310                 315                 320 


Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr Tyr Asp Asp His Tyr Ser 
                325                 330                 335     


Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val Thr Val Ser Ser Gly Gly 
            340                 345                 350         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        355                 360                 365             


Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
    370                 375                 380                 


Val Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser 
385                 390                 395                 400 


Tyr Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp 
                405                 410                 415     


Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser 
            420                 425                 430         


Gly Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln 
        435                 440                 445             


Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro 
    450                 455                 460                 


Phe Thr Phe Gly Cys Gly Thr Lys Leu Gln Ile Thr Arg 
465                 470                 475         


<210>  46
<211>  483
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2-BsAb light chain fusion polypeptide (25aa scFv linker)
       + disulfide mut

<400>  46

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Thr Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    210                 215                 220                 


Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Gln Ser Gly Gly 
225                 230                 235                 240 


Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Lys Ala Ser 
                245                 250                 255     


Gly Tyr Thr Phe Thr Arg Tyr Thr Met His Trp Val Arg Gln Ala Pro 
            260                 265                 270         


Gly Lys Cys Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr 
        275                 280                 285             


Thr Asn Tyr Asn Gln Lys Phe Lys Asp Arg Phe Thr Ile Ser Arg Asp 
    290                 295                 300                 


Asn Ser Lys Asn Thr Ala Phe Leu Gln Met Asp Ser Leu Arg Pro Glu 
305                 310                 315                 320 


Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr Tyr Asp Asp His Tyr Ser 
                325                 330                 335     


Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val Thr Val Ser Ser Gly Gly 
            340                 345                 350         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        355                 360                 365             


Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser 
    370                 375                 380                 


Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Ser Ala 
385                 390                 395                 400 


Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys 
                405                 410                 415     


Ala Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val 
            420                 425                 430         


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr 
        435                 440                 445             


Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln 
    450                 455                 460                 


Trp Ser Ser Asn Pro Phe Thr Phe Gly Gln Cys Gly Thr Lys Leu Gln 
465                 470                 475                 480 


Ile Thr Arg 
            


<210>  47
<211>  487
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2-BsAb light chain fusion polypeptide (30aa scFv linker)
       + disulfide mut

<400>  47

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Thr Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    210                 215                 220                 


Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Gln Ser Gly Gly 
225                 230                 235                 240 


Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Lys Ala Ser 
                245                 250                 255     


Gly Tyr Thr Phe Thr Arg Tyr Thr Met His Trp Val Arg Gln Ala Pro 
            260                 265                 270         


Gly Lys Cys Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr 
        275                 280                 285             


Thr Asn Tyr Asn Gln Lys Phe Lys Asp Arg Phe Thr Ile Ser Arg Asp 
    290                 295                 300                 


Asn Ser Lys Asn Thr Ala Phe Leu Gln Met Asp Ser Leu Arg Pro Glu 
305                 310                 315                 320 


Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr Tyr Asp Asp His Tyr Ser 
                325                 330                 335     


Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val Thr Val Ser Ser Gly Gly 
            340                 345                 350         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        355                 360                 365             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met 
    370                 375                 380                 


Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr 
385                 390                 395                 400 


Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln 
                405                 410                 415     


Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys 
            420                 425                 430         


Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 
        435                 440                 445             


Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr 
    450                 455                 460                 


Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr Phe Gly Cys Gly 
465                 470                 475                 480 


Thr Lys Leu Gln Ile Thr Arg 
                485         


<210>  48
<211>  231
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  huOKT3scFv(C105S) (5 aa scFv linker)

<400>  48

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Ser Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Pro Val Thr Val Ser Ser Gly Gly Gly Gly Ser Asp Ile Gln Met 
        115                 120                 125             


Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr 
    130                 135                 140                 


Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln 
145                 150                 155                 160 


Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys 
                165                 170                 175     


Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 
            180                 185                 190         


Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr 
        195                 200                 205             


Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr Phe Gly Gln Gly 
    210                 215                 220                 


Thr Lys Leu Gln Ile Thr Arg 
225                 230     


<210>  49
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  huOKT3scFv(C105S) (10 aa scFv linker)

<400>  49

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Ser Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Pro Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val 
    130                 135                 140                 


Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr 
145                 150                 155                 160 


Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile 
                165                 170                 175     


Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly 
            180                 185                 190         


Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 
        195                 200                 205             


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe 
    210                 215                 220                 


Thr Phe Gly Gln Gly Thr Lys Leu Gln Ile Thr Arg 
225                 230                 235     


<210>  50
<211>  246
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  huOKT3scFv(C105S) (20 aa scFv linker)

<400>  50

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Ser Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Pro Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr 
    130                 135                 140                 


Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile 
145                 150                 155                 160 


Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln 
                165                 170                 175     


Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu 
            180                 185                 190         


Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
        195                 200                 205             


Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr 
    210                 215                 220                 


Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr Phe Gly Gln Gly Thr 
225                 230                 235                 240 


Lys Leu Gln Ile Thr Arg 
                245     


<210>  51
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  huOKT3scFv(C105S) (25 aa scFv linker)

<400>  51

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Ser Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Pro Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
145                 150                 155                 160 


Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
                165                 170                 175     


Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile Tyr 
            180                 185                 190         


Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
        195                 200                 205             


Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu 
    210                 215                 220                 


Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr 
225                 230                 235                 240 


Phe Gly Gln Gly Thr Lys Leu Gln Ile Thr Arg 
                245                 250     


<210>  52
<211>  256
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  huOKT3scFv(C105S)  (30 aa scFv linker)

<400>  52

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Ser Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Pro Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu 
145                 150                 155                 160 


Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser 
                165                 170                 175     


Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro 
            180                 185                 190         


Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser 
        195                 200                 205             


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser 
    210                 215                 220                 


Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser 
225                 230                 235                 240 


Ser Asn Pro Phe Thr Phe Gly Gln Gly Thr Lys Leu Gln Ile Thr Arg 
                245                 250                 255     


<210>  53
<211>  231
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  huOKT3scFv(C105S) + disulfide mut (5 aa scFv linker)

<400>  53

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Cys Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Ser Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Pro Val Thr Val Ser Ser Gly Gly Gly Gly Ser Asp Ile Gln Met 
        115                 120                 125             


Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr 
    130                 135                 140                 


Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln 
145                 150                 155                 160 


Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys 
                165                 170                 175     


Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 
            180                 185                 190         


Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr 
        195                 200                 205             


Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr Phe Gly Cys Gly 
    210                 215                 220                 


Thr Lys Leu Gln Ile Thr Arg 
225                 230     


<210>  54
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  huOKT3scFv(C105S) + disulfide mut (10 aa scFv linker)

<400>  54

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Cys Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Ser Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Pro Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val 
    130                 135                 140                 


Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr 
145                 150                 155                 160 


Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile 
                165                 170                 175     


Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly 
            180                 185                 190         


Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 
        195                 200                 205             


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe 
    210                 215                 220                 


Thr Phe Gly Cys Gly Thr Lys Leu Gln Ile Thr Arg 
225                 230                 235     


<210>  55
<211>  241
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  huOKT3scFv(C105S) + disulfide mut (15 aa scFv linker)

<400>  55

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Cys Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Ser Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Pro Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
    130                 135                 140                 


Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser 
145                 150                 155                 160 


Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala 
                165                 170                 175     


Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro 
            180                 185                 190         


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile 
        195                 200                 205             


Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp 
    210                 215                 220                 


Ser Ser Asn Pro Phe Thr Phe Gly Cys Gly Thr Lys Leu Gln Ile Thr 
225                 230                 235                 240 


Arg 
    


<210>  56
<211>  246
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  huOKT3scFv(C105S) + disulfide mut (20 aa scFv linker)

<400>  56

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Cys Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Ser Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Pro Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr 
    130                 135                 140                 


Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile 
145                 150                 155                 160 


Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln 
                165                 170                 175     


Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu 
            180                 185                 190         


Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
        195                 200                 205             


Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr 
    210                 215                 220                 


Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr Phe Gly Cys Gly Thr 
225                 230                 235                 240 


Lys Leu Gln Ile Thr Arg 
                245     


<210>  57
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  huOKT3scFv(C105S) + disulfide mut (25 aa scFv linker)

<400>  57

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Cys Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Ser Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Pro Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
145                 150                 155                 160 


Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
                165                 170                 175     


Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile Tyr 
            180                 185                 190         


Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
        195                 200                 205             


Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu 
    210                 215                 220                 


Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr 
225                 230                 235                 240 


Phe Gly Gln Cys Gly Thr Lys Leu Gln Ile Thr Arg 
                245                 250         


<210>  58
<211>  256
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  huOKT3scFv(C105S) + disulfide mut (30 aa scFv linker)

<400>  58

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Cys Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Ser Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Pro Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu 
145                 150                 155                 160 


Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser 
                165                 170                 175     


Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro 
            180                 185                 190         


Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser 
        195                 200                 205             


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser 
    210                 215                 220                 


Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser 
225                 230                 235                 240 


Ser Asn Pro Phe Thr Phe Gly Cys Gly Thr Lys Leu Gln Ile Thr Arg 
                245                 250                 255     


<210>  59
<211>  241
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  huOKT3scFv + disulfide mut (15 aa scFv linker)

<400>  59

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Pro Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
    130                 135                 140                 


Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser 
145                 150                 155                 160 


Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala 
                165                 170                 175     


Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro 
            180                 185                 190         


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile 
        195                 200                 205             


Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp 
    210                 215                 220                 


Ser Ser Asn Pro Phe Thr Phe Gly Gln Gly Thr Lys Leu Gln Ile Thr 
225                 230                 235                 240 


Arg 
    


<210>  60
<211>  472
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Her2-BsAb light chain fusion polypeptide

<400>  60

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Thr Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    210                 215                 220                 


Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Gln Ser Gly Gly 
225                 230                 235                 240 


Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Lys Ala Ser 
                245                 250                 255     


Gly Tyr Thr Phe Thr Arg Tyr Thr Met His Trp Val Arg Gln Ala Pro 
            260                 265                 270         


Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr 
        275                 280                 285             


Thr Asn Tyr Asn Gln Lys Phe Lys Asp Arg Phe Thr Ile Ser Arg Asp 
    290                 295                 300                 


Asn Ser Lys Asn Thr Ala Phe Leu Gln Met Asp Ser Leu Arg Pro Glu 
305                 310                 315                 320 


Asp Thr Gly Val Tyr Phe Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys 
                325                 330                 335     


Leu Asp Tyr Trp Gly Gln Gly Thr Pro Val Thr Val Ser Ser Gly Gly 
            340                 345                 350         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln 
        355                 360                 365             


Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val 
    370                 375                 380                 


Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr 
385                 390                 395                 400 


Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile Tyr Asp Thr Ser 
                405                 410                 415     


Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
            420                 425                 430         


Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala 
        435                 440                 445             


Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr Phe Gly Gln 
    450                 455                 460                 


Gly Thr Lys Leu Gln Ile Thr Arg 
465                 470         


<210>  61
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Her2-bsab light chain sequence

<400>  61

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  62
<211>  450
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  her2 bsab heavy chain sequence

<400>  62

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr 
            20                  25                  30          


Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val 
        115                 120                 125             


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
    130                 135                 140                 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
145                 150                 155                 160 


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
                165                 170                 175     


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
            180                 185                 190         


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
        195                 200                 205             


Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp 
    210                 215                 220                 


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
225                 230                 235                 240 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
                245                 250                 255     


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
            260                 265                 270         


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
        275                 280                 285             


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr Arg 
    290                 295                 300                 


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
305                 310                 315                 320 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
                325                 330                 335     


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
            340                 345                 350         


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
        355                 360                 365             


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
    370                 375                 380                 


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
385                 390                 395                 400 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
                405                 410                 415     


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
            420                 425                 430         


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
        435                 440                 445             


Gly Lys 
    450 


<210>  63
<211>  450
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Trastuzumab heavy chain K322A

<400>  63

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr 
            20                  25                  30          


Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val 
        115                 120                 125             


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
    130                 135                 140                 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
145                 150                 155                 160 


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
                165                 170                 175     


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
            180                 185                 190         


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
        195                 200                 205             


Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp 
    210                 215                 220                 


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
225                 230                 235                 240 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
                245                 250                 255     


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
            260                 265                 270         


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
        275                 280                 285             


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
    290                 295                 300                 


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
305                 310                 315                 320 


Glu Tyr Lys Cys Ala Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
                325                 330                 335     


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
            340                 345                 350         


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
        355                 360                 365             


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
    370                 375                 380                 


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
385                 390                 395                 400 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
                405                 410                 415     


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
            420                 425                 430         


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
        435                 440                 445             


Gly Lys 
    450 


<210>  64
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  huOKT3 VH C105S + VH44

<400>  64

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Cys Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Ser Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Pro Val Thr Val Ser Ser 
        115                 


<210>  65
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  huOKT3 VL + VL100

<400>  65

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu 
65                  70                  75                  80  


Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr 
                85                  90                  95      


Phe Gly Cys Gly Thr Lys Leu Gln Ile Thr Arg 
            100                 105         


<210>  66
<211>  241
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  huOKT3 scFv; 15 aa linker

<400>  66

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Pro Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
    130                 135                 140                 


Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser 
145                 150                 155                 160 


Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala 
                165                 170                 175     


Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro 
            180                 185                 190         


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile 
        195                 200                 205             


Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp 
    210                 215                 220                 


Ser Ser Asn Pro Phe Thr Phe Gly Gln Gly Thr Lys Leu Gln Ile Thr 
225                 230                 235                 240 


Arg 
    

<210>  67
<211>  1416
<212>  DNA
<213>  Artififial Sequence

<220>
<223>  DNA encoding sequence of SEQ ID NO:60

<400>  67
gatattcaga tgactcagtc tccctcttcc ctgtccgctt cagtcggcga tcgggtcact       60

attacttgtc gggcttcaca ggatgtcaac acagccgtgg cttggtacca gcagaagccc      120

gggaaagcac ctaagctgct gatctactct gccagtttcc tgtattctgg cgtcccaagt      180

aggttttcag gctcccggag cggaactgac ttcaccctga caatttccag cctgcagccc      240

gaggattttg ctacctacta ttgccagcag cattatacta cccccccaac attcggccag      300

ggcacaaaag tcgaaatcaa gcggaccgtg gccgccccct ccgtgttcat cttccccccc      360

tccgacgagc agctgaagtc cggcaccgcc tccgtggtgt gcctgctgaa caacttctac      420

ccccgggagg ccaaggtgca gtggaaggtg gacaacgccc tgcagtccgg caactcccag      480

gagtccgtga ccgagcagga ctccaaggac tccacctact ccctgtcctc caccctgacc      540

ctgtccaagg ccgactacga gaagcacaag gtgtacgcct gcgaggtgac ccaccagggc      600

ctgtcctccc ccgtgaccaa gtccttcaac cggggcgagt gcactagtgg aggaggaggt      660

agcggaggag gaggttctgg cggagggggt tcccaggtgc agctggtgca gagcggagga      720

ggagtggtgc agccaggaag gagcctgcga ctgtcttgca aggctagtgg ctacaccttc      780

acacgatata ctatgcactg ggtgaggcag gcacctggta aaggcctgga gtggatcggc      840

tacattaacc cctctagggg atacaccaac tataatcaga agttcaaaga caggttcacc      900

atctcacgcg ataactccaa gaataccgcc ttcctgcaga tggactccct gcggcccgaa      960

gatacaggcg tgtatttttg cgctagatac tatgacgatc attactgtct ggactattgg     1020

ggacagggga cccctgtgac agtgtccagc ggtggaggag ggtcaggtgg aggagggagc     1080

ggtggcggag ggtctgacat ccagatgacc cagtccccat ctagtctgag cgcctctgtg     1140

ggcgatagag tgactattac ctgcagtgct tcatccagcg tgagctacat gaactggtat     1200

cagcagacac ccggaaaggc acctaaacgc tggatctacg atactagcaa gctggcctct     1260

ggcgtgccca gtcgattcag tggttcaggc tccggaaccg actatacctt caccatctct     1320

agtctgcagc ctgaggatat tgccacatac tattgtcagc agtggtcatc caatccattc     1380

acttttgggc agggtaccaa actgcagatt acaagg                               1416


<210>  68
<211>  1350
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encoding sequence of SEQ ID NO:62

<400>  68
gaagtgcagc tggtcgagag cggaggaggt ctggtgcagc ccggaggttc cctgagactg       60

tcctgtgccg catctgggtt taatatcaag gacacataca tccactgggt gagacaggca      120

cccggcaaag gactggagtg ggtcgccagg atctacccta ccaacgggta cacaagatat      180

gctgactctg tgaagggccg gttcaccatc tccgccgata ctagcaaaaa caccgcttac      240

ctgcagatga attccctgag ggcagaagat accgctgtct actactgttc aagatggggg      300

ggggatggtt tttacgctat ggattattgg ggccagggca ccctggtgac cgtgtcctcc      360

gcctccacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg      420

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg      480

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggccgtcct acagtcctca      540

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc      600

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagag agttgagccc      660

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact cctgggggga      720

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct      780

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg      840

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacgcc      900

agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag      960

gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc     1020

aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggatgag     1080

ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc     1140

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg     1200

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg     1260

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg     1320

cagaagagcc tctccctgtc tccgggtaaa                                      1350

<210>  69
<211>  1410
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HER2-C825 Light Chain

<400>  69
gatattcaga tgactcagtc tccctcttcc ctgtccgctt cagtcggcga tcgggtcact       60

attacttgtc gggcttcaca ggatgtcaac acagccgtgg cttggtacca gcagaagccc      120

gggaaagcac ctaagctgct gatctactct gccagtttcc tgtattctgg cgtcccaagt      180

aggttttcag gctcccggag cggaactgac ttcaccctga caatttccag cctgcagccc      240

gaggattttg ctacctacta ttgccagcag cattatacta cccccccaac attcggccag      300

ggcacaaaag tcgaaatcaa gcggaccgtg gccgccccct ccgtgttcat cttccccccc      360

tccgacgagc agctgaagtc cggcaccgcc tccgtggtgt gcctgctgaa caacttctac      420

ccccgggagg ccaaggtgca gtggaaggtg gacaacgccc tgcagtccgg caactcccag      480

gagtccgtga ccgagcagga ctccaaggac tccacctact ccctgtcctc caccctgacc      540

ctgtccaagg ccgactacga gaagcacaag gtgtacgcct gcgaggtgac ccaccagggc      600

ctgtcctccc ccgtgaccaa gtccttcaac cggggcgagt gcggtggtgg tggtagcggc      660

ggcggtggaa gcgcatccca tgtgaaactg caggaaagcg gcccaggtct ggtccagcca      720

tcccagtctc tgagcctgac atgcactgtg agcggattct ctctgacaga ctatggggtg      780

cactgggtca gacagagtcc aggaaagggg ctggagtggc tgggcgtcat ctggtcaggc      840

ggagggactg cttataacac cgcactgatc agcagactga atatctaccg cgacaactct      900

aaaaatcagg tgttcctgga gatgaacagt ctgcaggccg aagataccgc tatgtactat      960

tgcgccaggc ggggcagcta cccttataat tactttgacg cttggggttg tggcaccaca     1020

gtgacagtct ccagcggtgg aggagggagt ggtggaggag ggtcaggtgg aggagggtcc     1080

caggcagtgg tcattcagga gtctgccctg actacccccc ctggagaaac cgtgacactg     1140

acttgcggat ctagtacagg ggcagtgact gcctccaact atgcaaattg ggtccaggaa     1200

aagcctgatc actgtttcac tggcctgatc ggtggccata acaatcgacc acccggagtg     1260

ccagctaggt tttcaggttc cctgatcggc gacaaagccg ctctgaccat tgctggcacc     1320

cagacagagg atgaagcaat ctacttttgt gccctgtggt attccgatca ctgggtcatt     1380

ggggggggga cacgtctgac tgtgctgggg                                      1410


<210>  70
<211>  1350
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HER2-C825 Heavy Chain

<400>  70
gaagtgcagc tggtcgagag cggaggaggt ctggtgcagc ccggaggttc cctgagactg       60

tcctgtgccg catctgggtt taatatcaag gacacataca tccactgggt gagacaggca      120

cccggcaaag gactggagtg ggtcgccagg atctacccta ccaacgggta cacaagatat      180

gctgactctg tgaagggccg gttcaccatc tccgccgata ctagcaaaaa caccgcttac      240

ctgcagatga attccctgag ggcagaagat accgctgtct actactgttc aagatggggg      300

ggggatggtt tttacgctat ggattattgg ggccagggca ccctggtgac cgtgtcctcc      360

gcctccacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg      420

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg      480

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggccgtcct acagtcctca      540

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc      600

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagag agttgagccc      660

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact cctgggggga      720

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct      780

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg      840

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacgcc      900

agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag      960

gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc     1020

aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggatgag     1080

ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc     1140

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg     1200

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg     1260

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg     1320

cagaagagcc tctccctgtc tccgggtaaa                                      1350


<210>  71
<211>  470
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2-C825 Light Chain sequence

<400>  71

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    210                 215                 220                 


Ala Ser His Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Gln Pro 
225                 230                 235                 240 


Ser Gln Ser Leu Ser Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Thr 
                245                 250                 255     


Asp Tyr Gly Val His Trp Val Arg Gln Ser Pro Gly Lys Gly Leu Glu 
            260                 265                 270         


Trp Leu Gly Val Ile Trp Ser Gly Gly Gly Thr Ala Tyr Asn Thr Ala 
        275                 280                 285             


Leu Ile Ser Arg Leu Asn Ile Tyr Arg Asp Asn Ser Lys Asn Gln Val 
    290                 295                 300                 


Phe Leu Glu Met Asn Ser Leu Gln Ala Glu Asp Thr Ala Met Tyr Tyr 
305                 310                 315                 320 


Cys Ala Arg Arg Gly Ser Tyr Pro Tyr Asn Tyr Phe Asp Ala Trp Gly 
                325                 330                 335     


Cys Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
            340                 345                 350         


Gly Gly Ser Gly Gly Gly Gly Ser Gln Ala Val Val Ile Gln Glu Ser 
        355                 360                 365             


Ala Leu Thr Thr Pro Pro Gly Glu Thr Val Thr Leu Thr Cys Gly Ser 
    370                 375                 380                 


Ser Thr Gly Ala Val Thr Ala Ser Asn Tyr Ala Asn Trp Val Gln Glu 
385                 390                 395                 400 


Lys Pro Asp His Cys Phe Thr Gly Leu Ile Gly Gly His Asn Asn Arg 
                405                 410                 415     


Pro Pro Gly Val Pro Ala Arg Phe Ser Gly Ser Leu Ile Gly Asp Lys 
            420                 425                 430         


Ala Ala Leu Thr Ile Ala Gly Thr Gln Thr Glu Asp Glu Ala Ile Tyr 
        435                 440                 445             


Phe Cys Ala Leu Trp Tyr Ser Asp His Trp Val Ile Gly Gly Gly Thr 
    450                 455                 460                 


Arg Leu Thr Val Leu Gly 
465                 470 


<210>  72
<211>  450
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2-C825 Heavy Chain sequence

<400>  72

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr 
            20                  25                  30          


Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val 
        115                 120                 125             


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
    130                 135                 140                 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
145                 150                 155                 160 


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
                165                 170                 175     


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
            180                 185                 190         


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
        195                 200                 205             


Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp 
    210                 215                 220                 


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
225                 230                 235                 240 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
                245                 250                 255     


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
            260                 265                 270         


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
        275                 280                 285             


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr Arg 
    290                 295                 300                 


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
305                 310                 315                 320 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
                325                 330                 335     


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
            340                 345                 350         


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
        355                 360                 365             


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
    370                 375                 380                 


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
385                 390                 395                 400 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
                405                 410                 415     


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
            420                 425                 430         


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
        435                 440                 445             


Gly Lys 
    450 