                         SEQUENCE LISTING

<110>  VIB VZW
       KATHOLIEKE UNIVERSITEIT LEUVEN, K.U.LEUVEN R&D
 
<120>  Reducing acetate ester production in yeast

<130>  KV/AROMA/578

<150>  EP 17153814.3
<151>  2017-01-30

<160>  31    

<170>  PatentIn version 3.5

<210>  1
<211>  468
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  1
atgacggttg gtacacttcc catctggtca aaaacctttt cttatgcaat taggtcatta       60

tgcttcttgc atataataca tatgtatgca tacgaattta ctgagacgag gggagaatca      120

atgttgccaa cactgtcagc gaccaatgat tatgtgcatg tcttgaaaaa tttccaaaat      180

ggtagaggca taaaaatggg tgactgcata gtagcactga aacctactga ccctaatcat      240

aggatttgca aaagggttac gggtatgcct ggtgatctcg tgctcgtgga tcccagtacg      300

atagttaatt atgtcggcga tgtgcttgtt gatgaggaga ggtttggtac ctatattaaa      360

gtccctgaag gtcatgtttg ggtaaccgga gataatttgt cacattcatt agattcaaga      420

acatacaatg cattacccat ggggctgatc atgggtaatg cattgtag                   468


<210>  2
<211>  155
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  2

Met Thr Val Gly Thr Leu Pro Ile Trp Ser Lys Thr Phe Ser Tyr Ala 
1               5                   10                  15      


Ile Arg Ser Leu Cys Phe Leu His Ile Ile His Met Tyr Ala Tyr Glu 
            20                  25                  30          


Phe Thr Glu Thr Arg Gly Glu Ser Met Leu Pro Thr Leu Ser Ala Thr 
        35                  40                  45              


Asn Asp Tyr Val His Val Leu Lys Asn Phe Gln Asn Gly Arg Gly Ile 
    50                  55                  60                  


Lys Met Gly Asp Cys Ile Val Ala Leu Lys Pro Thr Asp Pro Asn His 
65                  70                  75                  80  


Arg Ile Cys Lys Arg Val Thr Gly Met Pro Gly Asp Leu Val Leu Val 
                85                  90                  95      


Asp Pro Ser Thr Ile Val Asn Tyr Val Gly Asp Val Leu Val Asp Glu 
            100                 105                 110         


Glu Arg Phe Gly Thr Tyr Ile Lys Val Pro Glu Gly His Val Trp Val 
        115                 120                 125             


Thr Gly Asp Asn Leu Ser His Ser Leu Asp Ser Arg Thr Tyr Asn Ala 
    130                 135                 140                 


Leu Pro Met Gly Leu Ile Met Gly Asn Ala Leu 
145                 150                 155 


<210>  3
<211>  573
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  3
atgacggttg gtacacttcc catctggtca aaaacctttt cttatgcaat taggtcatta       60

tgcttcttgc atataataca tatgtatgca tacgaattta ctgagacgag gggagaatca      120

atgttgccaa cactgtcagc gaccaatgat tatgtgcatg tcttgaaaaa tttccaaaat      180

ggtagaggca taaaaatggg tgactgcata gtagcactga aacctactga ccctaatcat      240

aggatttgca aaagggttac gggtatgcct ggtgatctcg tgctcgtgga tcccagtacg      300

atagttaatt atgtcggcga tgtgcttgtt gatgaggaga ggtttggtac ctatattaaa      360

gtccctgaag gtcatgtttg ggtaaccgga gataatttgt cacattcatt agattcaaga      420

acatacaatg cattacccat ggggctgatc atgggtaaga ttgtagctgc taacaatttt      480

gacaagccct tctgggacgg ttcgatacgc aatatttggg gtttcaaatg gatcaataat      540

acatttctag atgtgcaggc taagagcaac tga                                   573


<210>  4
<211>  190
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  4

Met Thr Val Gly Thr Leu Pro Ile Trp Ser Lys Thr Phe Ser Tyr Ala 
1               5                   10                  15      


Ile Arg Ser Leu Cys Phe Leu His Ile Ile His Met Tyr Ala Tyr Glu 
            20                  25                  30          


Phe Thr Glu Thr Arg Gly Glu Ser Met Leu Pro Thr Leu Ser Ala Thr 
        35                  40                  45              


Asn Asp Tyr Val His Val Leu Lys Asn Phe Gln Asn Gly Arg Gly Ile 
    50                  55                  60                  


Lys Met Gly Asp Cys Ile Val Ala Leu Lys Pro Thr Asp Pro Asn His 
65                  70                  75                  80  


Arg Ile Cys Lys Arg Val Thr Gly Met Pro Gly Asp Leu Val Leu Val 
                85                  90                  95      


Asp Pro Ser Thr Ile Val Asn Tyr Val Gly Asp Val Leu Val Asp Glu 
            100                 105                 110         


Glu Arg Phe Gly Thr Tyr Ile Lys Val Pro Glu Gly His Val Trp Val 
        115                 120                 125             


Thr Gly Asp Asn Leu Ser His Ser Leu Asp Ser Arg Thr Tyr Asn Ala 
    130                 135                 140                 


Leu Pro Met Gly Leu Ile Met Gly Lys Ile Val Ala Ala Asn Asn Phe 
145                 150                 155                 160 


Asp Lys Pro Phe Trp Asp Gly Ser Ile Arg Asn Ile Trp Gly Phe Lys 
                165                 170                 175     


Trp Ile Asn Asn Thr Phe Leu Asp Val Gln Ala Lys Ser Asn 
            180                 185                 190 


<210>  5
<211>  147
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  5

Met Thr Val Gly Thr Leu Pro Ile Trp Ser Lys Thr Phe Ser Tyr Ala 
1               5                   10                  15      


Ile Arg Ser Leu Cys Phe Leu His Ile Ile His Met Tyr Ala Tyr Glu 
            20                  25                  30          


Phe Thr Glu Thr Arg Gly Glu Ser Met Leu Pro Thr Leu Ser Ala Thr 
        35                  40                  45              


Asn Asp Tyr Val His Val Leu Lys Asn Phe Gln Asn Gly Arg Gly Ile 
    50                  55                  60                  


Lys Met Gly Asp Cys Ile Val Ala Leu Lys Pro Thr Asp Pro Asn His 
65                  70                  75                  80  


Arg Ile Cys Lys Arg Val Thr Gly Met Pro Gly Asp Leu Val Leu Val 
                85                  90                  95      


Asp Pro Ser Thr Ile Val Asn Tyr Val Gly Asp Val Leu Val Asp Glu 
            100                 105                 110         


Glu Arg Phe Gly Thr Tyr Ile Lys Val Pro Glu Gly His Val Trp Val 
        115                 120                 125             


Thr Gly Asp Asn Leu Ser His Ser Leu Asp Ser Arg Thr Tyr Asn Ala 
    130                 135                 140                 


Leu Pro Met 
145         


<210>  6
<211>  147
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  6

Met Thr Val Gly Thr Leu Pro Ile Trp Ser Lys Thr Phe Ser Tyr Ala 
1               5                   10                  15      


Ile Arg Ser Leu Cys Phe Leu His Ile Ile His Met Tyr Ala Tyr Glu 
            20                  25                  30          


Phe Thr Glu Thr Arg Gly Glu Ser Met Leu Pro Thr Leu Ser Ala Thr 
        35                  40                  45              


Asn Asp Tyr Val His Val Leu Lys Asn Phe Gln Asn Gly Arg Gly Ile 
    50                  55                  60                  


Lys Met Gly Asp Cys Ile Val Ala Leu Lys Pro Thr Asp Pro Asn His 
65                  70                  75                  80  


Arg Ile Cys Lys Arg Val Thr Gly Met Pro Gly Asp Leu Val Leu Val 
                85                  90                  95      


Asp Pro Ser Thr Ile Val Asn Tyr Val Gly Asp Val Leu Val Asp Glu 
            100                 105                 110         


Glu Arg Phe Gly Thr Tyr Ile Lys Val Pro Glu Gly His Val Trp Val 
        115                 120                 125             


Thr Gly Asp Asn Leu Ser His Ser Leu Asp Ser Arg Thr Tyr Asn Ala 
    130                 135                 140                 


Leu Pro Met 
145         


<210>  7
<211>  167
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  7

Met Thr Val Gly Thr Leu Pro Ile Trp Ser Lys Thr Phe Ser Tyr Ala 
1               5                   10                  15      


Ile Arg Ser Leu Cys Phe Leu His Ile Ile His Met Tyr Ala Tyr Glu 
            20                  25                  30          


Phe Thr Glu Thr Arg Gly Glu Ser Met Leu Pro Thr Leu Ser Ala Thr 
        35                  40                  45              


Asn Asp Tyr Val His Val Leu Lys Asn Phe Gln Asn Gly Arg Gly Ile 
    50                  55                  60                  


Lys Met Gly Asp Cys Ile Val Ala Leu Lys Pro Thr Asp Pro Asn His 
65                  70                  75                  80  


Arg Ile Cys Lys Arg Val Thr Gly Met Pro Gly Asp Leu Val Leu Val 
                85                  90                  95      


Asp Pro Ser Thr Ile Val Asn Tyr Val Gly Asp Val Leu Val Asp Glu 
            100                 105                 110         


Glu Arg Phe Gly Thr Tyr Ile Lys Val Pro Glu Gly His Val Trp Val 
        115                 120                 125             


Thr Gly Asp Asn Leu Ser His Ser Leu Asp Ser Arg Thr Tyr Asn Ala 
    130                 135                 140                 


Leu Pro Met Gly Leu Ile Met Gly Lys Ile Val Ala Ala Asn Asn Phe 
145                 150                 155                 160 


Asp Lys Pro Phe Trp Asp Gly 
                165         


<210>  8
<211>  172
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  8

Met Thr Val Gly Thr Leu Pro Ile Trp Ser Lys Thr Phe Glu Phe Thr 
1               5                   10                  15      


Glu Thr Arg Gly Glu Ser Met Leu Pro Thr Leu Ser Ala Thr Asn Asp 
            20                  25                  30          


Tyr Val His Val Leu Lys Asn Phe Gln Asn Gly Arg Gly Ile Lys Met 
        35                  40                  45              


Gly Asp Cys Ile Val Ala Leu Lys Pro Thr Asp Pro Asn His Arg Ile 
    50                  55                  60                  


Cys Lys Arg Val Thr Gly Met Pro Gly Asp Leu Val Leu Val Asp Pro 
65                  70                  75                  80  


Ser Thr Ile Val Asn Tyr Val Gly Asp Val Leu Val Asp Glu Glu Arg 
                85                  90                  95      


Phe Gly Thr Tyr Ile Lys Val Pro Glu Gly His Val Trp Val Thr Gly 
            100                 105                 110         


Asp Asn Leu Ser His Ser Leu Asp Ser Arg Thr Tyr Asn Ala Leu Pro 
        115                 120                 125             


Met Gly Leu Ile Met Gly Lys Ile Val Ala Ala Asn Asn Phe Asp Lys 
    130                 135                 140                 


Pro Phe Trp Asp Gly Ser Ile Arg Asn Ile Trp Gly Phe Lys Trp Ile 
145                 150                 155                 160 


Asn Asn Thr Phe Leu Asp Val Gln Ala Lys Ser Asn 
                165                 170         


<210>  9
<211>  345
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  9
atgaatgaaa tcgatgagaa aaatcaggcc cccgtgcaac aagaatgcct gaaagagatg       60

attcagaatg ggcatgctcg gcgtatggga tctgttgaag atctgtatgt tgctctcaac      120

agacaaaact tatatcgaaa cttctgcaca tatggagaat tgagtgatta ctgtactagg      180

gatcagctca cattagcttt gaaggaaatc tgcctgaaaa tccaactctt ttacatattg      240

ttctaccaac aagatggcca aatcatgaaa attattatcg cagttccgaa tactattcac      300

ggccacatcc agtgcatgat tatatttcag tattacaaga attga                      345


<210>  10
<211>  1577
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  10
atgaatgaaa tcgatgagaa aaatcaggcc cccgtgcaac aagaatgcct gaaagagatg       60

attcagaatg ggcatgctcg gcgtatggga tctgttgaag atctgtatgt tgctctcaac      120

agacaaaact tatatcgaaa cttctgcaca tatggagaat tgagtgatta ctgtactagg      180

gatcagctca cattagcttt gaaggaaatc tgcctgaaaa tccaactctt ttacatattg      240

ttctaccaac aagatggcca aatcatgaaa attattatcg cagttccgaa tactattcac      300

ggccacatcc agtgcatgat tatatttcag tattacaaga attgaaactg agtggtgtgg      360

ttctcaatga acaacctgag tacagtgcag taatgaagca aatattagaa gaattcaaaa      420

atagtaaggg ttcctatact gcaaaaattt ttaaacttac taccactttg actattcctt      480

actttggacc aacaggaccg agttggcggc taatttgtct tccagaagag cacacagaaa      540

agtggaaaaa atttatcttt gtatctaatc attgcatgtc tgatggtcgg tcttcgatcc      600

acttttttca tgatttaaga gacgaattaa ataatattaa aactccacca aaaaaattag      660

attacatttt caagtacgag gaggattacc aattattgag gaaacttcca gaaccgatcg      720

aaaaggtgat agactttaga ccaccgtact tgtttattcc gaagtcactt ctttcgggtt      780

tcatctacaa tcatttgaga ttttcttcaa aaggtgtctg tatgagaatg gatgatgtgg      840

aaaaaaccga tgatgttgtc accgagatca tcaatatttc accaacagaa tttcaagcga      900

ttaaagcaaa tattaaatca aatatccaag gtaagtgtac tatcactccg tttttacatg      960

tttgttggtt tgtatctctt cataaatggg gtaaattttt caaaccattg aacttcgaat     1020

ggcttacgga tatttttatc cccgcagatt gccgctcaca actaccagat gatgatgaaa     1080

tgagacagat gtacagatat ggcgctaacg ttggatttat tgacttcacc ccctggataa     1140

gcgaatttga catgaatgat aacaaagaaa atttttggcc acttattgag cactaccatg     1200

aagtaatttc ggaagcttta agaaataaaa agcatctcca tggcttaggg ttcaatatac     1260

aaggcttcgt tcaaaaatat gtgaacattg acaaggtaat gtgcgatcgt gccatcggga     1320

aaagacgcgg aggtacattg ttaagcaatg taggtctgtt taatcagtta gaggagcccg     1380

atgccaaata ttctatatgc gatttggcat ttggccaatt tcaaggatcc tggcaccaag     1440

cattttcctt gggtgtttgt tcgactaatg taaaggggat gaatattgtt gttgcttcaa     1500

caaagaatgt tgttggtagt caagaatctc tcgaagagct ttgctccatt tacaaagctc     1560

tccttttagg cccttag                                                    1577


<210>  11
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP1 FW primer

<400>  11
ccaaatattg cgtatcgaac cgtcccagaa gggcttgtca aaattgttag cagctacaat       60

ctacgctgca ggtcgacaa                                                    79


<210>  12
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP1 RV primer

<400>  12
ggagataatt tgtcacattc attagattca agaacataca atgcattacc catggggctg       60

acgttggccg attcattaa                                                    79


<210>  13
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATF1 FW primer

<400>  13
ggacattgag ctaaggttca atgcactcga tggtcttctc acttccgaat atatagatct       60

agctacgctg caggtcgaca a                                                 81


<210>  14
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATF1 RV primer

<400>  14
ggacgacgat tctgaccctt tctatttaaa tagctcctta catcgagaag atctctgcag       60

ccgttggccg attcattaa                                                    79


<210>  15
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP2 FW primer

<400>  15
tagcaatttc atgggttccg gtacttctaa caatcaataa taatgtggtc cagctgaagc       60

ttcgtacgc                                                               69


<210>  16
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP2 RV primer

<400>  16
gttgccagcg tttccgtttg cgggttcagc gtaggctgca tagaggtacc gtgcttgggt       60

gttttgaagt gg                                                           72


<210>  17
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SOM1 FW primer

<400>  17
gtgtccgtga cctcgtagtt agtggctgat ttgtcgggtg caatgcagtg gtgcttgggt       60

gttttgaagt gg                                                           72


<210>  18
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SOM1 RV primer

<400>  18
ggagtgccaa ttcaaaggag ccgaatacgt ctgctcgcct tttaagaggc cagctgaagc       60

ttcgtacgc                                                               69


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP1 gDNA

<400>  19
attacccatg gggctgatca                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATF1 gDNA

<400>  20
agaacaatat gtaaaagagt                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP1truncA gDNA

<400>  21
catacaatgc attacccatg                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP1truncB gDNA

<400>  22
acggttcgat acgcaatatt                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP1truncC gDNA

<400>  23
aaaccttttc ttatgcaatt                                                   20


<210>  24
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP1 repair template

<400>  24
gtatcgaacc gtcccagaag ggcttgtcaa aattgttagc agctacaatg cattgcccat       60

gatcagcccc atgggtaatg cattgtatgt tcttgaatct                            100


<210>  25
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATF1 repair template

<400>  25
ctgtactagg gatcagctca cattagcttt gaaggaaatc tgcctgaaaa tccaactctt       60

ttacatattg ttctaccaac aagatggcca aatcatgaa                              99


<210>  26
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP1truncA repair template

<400>  26
cggagataat ttgtcacatt cattagattc aagaacatac aatgcattac ccatgtagct       60

gatcatgggc aagattgtag ctgctaacaa ttttgacaag                            100


<210>  27
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP1truncB repair template

<400>  27
aagattgtag ctgctaacaa ttttgacaag cccttctggg acggttagat acgcaatatt       60

tggggtttca aatggatcaa taatacattt ctagatgtgc                            100


<210>  28
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP1truncC repair template

<400>  28
gtgtacaata ccagggatga cggttggtac acttcccatc tggtcaaaaa cctttgaatt       60

tactgagacg aggggagaat caatgttgcc aacactgtca                            100


<210>  29
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  29
actccacttc aagtaagagt t                                                 21


<210>  30
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  30
gcacggaata tgggactact tcg                                               23


<210>  31
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  31
agtcacatca agatcgttta tgg                                               23


