                         SEQUENCE LISTING

<110>  The Donald Danforth Plant Science Center
       Kutchan, Toni
       Augustin, Megan
 
<120>  Morphinan N-Demethylase (MND) Isolated from the Methylbacterium 
       Thebainefresser and Methods of Use Thereof

<130>  47704-158297/PROV

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  2013
<212>  DNA
<213>  Methylobacterium Thebainfresser

<400>  1
atgactgaaa aaacgcctaa actaggctct gaagccgcaa ataaactcgg attagaagct       60

gacatttctc ggcgcgacat ggtgggcggc gtattgatcg gagcaggggc agctctgctc      120

gcatccgttg cgccaggggc gattaataga gcgttggccg aggggccgag caggctaccg      180

ccggtgcgtg gttccggtac cggttggcga ggaatagaga tcgccgatga ctggcaaggc      240

ccaggcggca tcggggatta ctctaagtca aacggcaaca cgggcaaagt tatccgtgac      300

gcgcatgcag gcattcggaa ccacgagttc gagaagcggc ttgctacggc gagtgacgtc      360

aacgagaaat atgatgttat tattgtaggc gcgggcattt cggggcttca cagcgcctac      420

gatcttcttc gccagcggcc aaatataaag atcctaatgc ttgacaacca tgccatcttc      480

ggtggggagg caaagcaaaa taagatggag gtagacggtc aggcgctgta tggcggccag      540

ggcccaacgc tttactcctt cgttggtgat gacctcccga gctggaaagg taatccggcc      600

ctcgcatcta tcatggaact caaaacatat cccaaggagt tcggacttcc gaccgaaact      660

acatggagcg ataagaagac ggacgtaaag gtgccggtcg acctttggtt ctccatggcc      720

agcccctcgc agaccgacat cgcctatcgg tgggagggaa gtgggttggt gaagaatcct      780

ttgctgaact cctttcgtga tgccccagtg tcgcagaaaa gcaaagatgc cattgccctt      840

atgctcgctg tcgacaacgg cgcgaagagg cctgttgaac cagtgggcga tgtatcgacc      900

tgggtcgaca atatgaccta tgcggagttt ctgaagaagg tatatggtgc ggacgacgaa      960

gctgttcagc ttgtcgacca gattgatgtc gttggaacag cggggcttgg cggtgatgtt     1020

tttaatgcaa gccttgcagc actcggacta aatcaatacg ggggcatcga gctctggaac     1080

ggcggtttgc aagggttgag tcttccgacc ggaaatggcg gcgtggggcg gtccatcctt     1140

cgcaagttca tgccaggagc tatcaagggc gggacatcgc tcaccgacac gcttttcggt     1200

gacgtgaact gggacgtgct tgaccacgct aacaacaacg ttcgaatccg gctcaattca     1260

actgtcgtag gtgttcagaa taatgagacg ccgactggca caaaagatgc gaccgttttc     1320

ttccttcacg ataatcgcct ttacaaggcc aaagggaagg cggtgatcat gggtacaccg     1380

cagcaggtca atcgtaatgt ttgcctcaat ttgccaaacc atcttagcga ggcaatgggc     1440

gatttccatc atgctccgat cctggttgtg aatgtggccc tccggaactg gaaatcgatg     1500

gaaaaggctg gcgtttccgg cttgcggtgg ttcggagaat atccgggtat cggtcagata     1560

gttcgatcga tggtcattga cggcaaagag atcatgcctt gcgatccctc gaaaccagcg     1620

gtcatgacct tctatatccc gatgaatcaa gcgacacggg gcatgcctcg cggcgagcaa     1680

gcgatgaccg cccgccacat gcttttcaac ttgacgttcg cagatatcga actgctcatt     1740

cgggatcagc tcactcgtgc gttcggatct tatggatttg atgccaagcg ggacatcgct     1800

gccattgttg caaacaggtg gggacatgcg ctggtctgcg ccgggccagg attttacact     1860

gggcttaacg gcaaaccgcc cgtcagtaag gtgatcaccg ctggatggga ccgagtggca     1920

ttcgggcatt cggacctttc cggcagacaa gcatggaccg tggccgtaaa ttatgcacgg     1980

acagcggttg cgaatgtctt ccctaaaatc tga                                  2013


<210>  2
<211>  670
<212>  PRT
<213>  Methylobacterium Thebainfresser

<400>  2

Met Thr Glu Lys Thr Pro Lys Leu Gly Ser Glu Ala Ala Asn Lys Leu 
1               5                   10                  15      


Gly Leu Glu Ala Asp Ile Ser Arg Arg Asp Met Val Gly Gly Val Leu 
            20                  25                  30          


Ile Gly Ala Gly Ala Ala Leu Leu Ala Ser Val Ala Pro Gly Ala Ile 
        35                  40                  45              


Asn Arg Ala Leu Ala Glu Gly Pro Ser Arg Leu Pro Pro Val Arg Gly 
    50                  55                  60                  


Ser Gly Thr Gly Trp Arg Gly Ile Glu Ile Ala Asp Asp Trp Gln Gly 
65                  70                  75                  80  


Pro Gly Gly Ile Gly Asp Tyr Ser Lys Ser Asn Gly Asn Thr Gly Lys 
                85                  90                  95      


Val Ile Arg Asp Ala His Ala Gly Ile Arg Asn His Glu Phe Glu Lys 
            100                 105                 110         


Arg Leu Ala Thr Ala Ser Asp Val Asn Glu Lys Tyr Asp Val Ile Ile 
        115                 120                 125             


Val Gly Ala Gly Ile Ser Gly Leu His Ser Ala Tyr Asp Leu Leu Arg 
    130                 135                 140                 


Gln Arg Pro Asn Ile Lys Ile Leu Met Leu Asp Asn His Ala Ile Phe 
145                 150                 155                 160 


Gly Gly Glu Ala Lys Gln Asn Lys Met Glu Val Asp Gly Gln Ala Leu 
                165                 170                 175     


Tyr Gly Gly Gln Gly Pro Thr Leu Tyr Ser Phe Val Gly Asp Asp Leu 
            180                 185                 190         


Pro Ser Trp Lys Gly Asn Pro Ala Leu Ala Ser Ile Met Glu Leu Lys 
        195                 200                 205             


Thr Tyr Pro Lys Glu Phe Gly Leu Pro Thr Glu Thr Thr Trp Ser Asp 
    210                 215                 220                 


Lys Lys Thr Asp Val Lys Val Pro Val Asp Leu Trp Phe Ser Met Ala 
225                 230                 235                 240 


Ser Pro Ser Gln Thr Asp Ile Ala Tyr Arg Trp Glu Gly Ser Gly Leu 
                245                 250                 255     


Val Lys Asn Pro Leu Leu Asn Ser Phe Arg Asp Ala Pro Val Ser Gln 
            260                 265                 270         


Lys Ser Lys Asp Ala Ile Ala Leu Met Leu Ala Val Asp Asn Gly Ala 
        275                 280                 285             


Lys Arg Pro Val Glu Pro Val Gly Asp Val Ser Thr Trp Val Asp Asn 
    290                 295                 300                 


Met Thr Tyr Ala Glu Phe Leu Lys Lys Val Tyr Gly Ala Asp Asp Glu 
305                 310                 315                 320 


Ala Val Gln Leu Val Asp Gln Ile Asp Val Val Gly Thr Ala Gly Leu 
                325                 330                 335     


Gly Gly Asp Val Phe Asn Ala Ser Leu Ala Ala Leu Gly Leu Asn Gln 
            340                 345                 350         


Tyr Gly Gly Ile Glu Leu Trp Asn Gly Gly Leu Gln Gly Leu Ser Leu 
        355                 360                 365             


Pro Thr Gly Asn Gly Gly Val Gly Arg Ser Ile Leu Arg Lys Phe Met 
    370                 375                 380                 


Pro Gly Ala Ile Lys Gly Gly Thr Ser Leu Thr Asp Thr Leu Phe Gly 
385                 390                 395                 400 


Asp Val Asn Trp Asp Val Leu Asp His Ala Asn Asn Asn Val Arg Ile 
                405                 410                 415     


Arg Leu Asn Ser Thr Val Val Gly Val Gln Asn Asn Glu Thr Pro Thr 
            420                 425                 430         


Gly Thr Lys Asp Ala Thr Val Phe Phe Leu His Asp Asn Arg Leu Tyr 
        435                 440                 445             


Lys Ala Lys Gly Lys Ala Val Ile Met Gly Thr Pro Gln Gln Val Asn 
    450                 455                 460                 


Arg Asn Val Cys Leu Asn Leu Pro Asn His Leu Ser Glu Ala Met Gly 
465                 470                 475                 480 


Asp Phe His His Ala Pro Ile Leu Val Val Asn Val Ala Leu Arg Asn 
                485                 490                 495     


Trp Lys Ser Met Glu Lys Ala Gly Val Ser Gly Leu Arg Trp Phe Gly 
            500                 505                 510         


Glu Tyr Pro Gly Ile Gly Gln Ile Val Arg Ser Met Val Ile Asp Gly 
        515                 520                 525             


Lys Glu Ile Met Pro Cys Asp Pro Ser Lys Pro Ala Val Met Thr Phe 
    530                 535                 540                 


Tyr Ile Pro Met Asn Gln Ala Thr Arg Gly Met Pro Arg Gly Glu Gln 
545                 550                 555                 560 


Ala Met Thr Ala Arg His Met Leu Phe Asn Leu Thr Phe Ala Asp Ile 
                565                 570                 575     


Glu Leu Leu Ile Arg Asp Gln Leu Thr Arg Ala Phe Gly Ser Tyr Gly 
            580                 585                 590         


Phe Asp Ala Lys Arg Asp Ile Ala Ala Ile Val Ala Asn Arg Trp Gly 
        595                 600                 605             


His Ala Leu Val Cys Ala Gly Pro Gly Phe Tyr Thr Gly Leu Asn Gly 
    610                 615                 620                 


Lys Pro Pro Val Ser Lys Val Ile Thr Ala Gly Trp Asp Arg Val Ala 
625                 630                 635                 640 


Phe Gly His Ser Asp Leu Ser Gly Arg Gln Ala Trp Thr Val Ala Val 
                645                 650                 655     


Asn Tyr Ala Arg Thr Ala Val Ala Asn Val Phe Pro Lys Ile 
            660                 665                 670 


<210>  3
<211>  2013
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  3
atgactgaga agacacctaa acttggatct gaggcagcta ataagttggg tttggaggct       60

gacatctcta ggagagatat ggtgggagga gtgcttatcg gtgcaggtgc tgctttgctt      120

gcatctgttg ctcctggtgc tatcaacagg gctcttgcag aaggaccatc aaggcttcct      180

ccagttagag gttcaggaac tggatggagg ggtattgaga ttgcagacga ctggcaagga      240

cctggaggaa tcggagatta ttctaagtct aacggtaaca ctggaaaggt gatcagggac      300

gctcacgctg gtatcaggaa ccatgagttc gaaaagaggt tggctactgc ttctgacgtg      360

aacgagaagt acgacgtgat catcgtggga gctggtatct ctggacttca ctctgcatac      420

gatttgttga gacaaagacc taatattaaa attttaatgt tggacaacca cgctatcttc      480

ggtggtgagg ctaagcagaa caagatggag gttgacggtc aggcattgta cggaggacag      540

ggaccaactc tttactcatt cgttggagac gatttgcctt catggaaggg aaaccctgct      600

ttggcatcta tcatggaact taagacatat ccaaaggagt tcggacttcc aactgagact      660

acttggtcag ataagaagac tgacgttaag gttccagtgg acctttggtt ctcaatggca      720

tcaccttcac agacagatat tgcttacagg tgggagggtt ctggtttggt gaaaaatcct      780

ttgcttaact ctttcaggga tgctccagtt tctcagaagt caaaggatgc tatcgctttg      840

atgcttgcag tggacaacgg tgctaagaga cctgtggaac ctgttggaga cgtgtctaca      900

tgggtggaca acatgacata cgctgagttt ttgaagaagg tgtacggagc agatgatgag      960

gcagttcagt tggtggacca gatcgacgtt gtgggtacag ctggacttgg tggagacgtg     1020

ttcaacgcat cacttgctgc tttgggtttg aaccagtacg gtggaatcga gttgtggaac     1080

ggaggattgc agggtctttc tttgccaaca ggtaatggag gagtgggtag gtctatcctt     1140

aggaagttca tgcctggtgc tattaagggt ggaacatctt tgacagatac tcttttcggt     1200

gacgtgaact gggacgtttt ggatcacgca aacaacaatg tgagaattag acttaattct     1260

acagtggttg gagtgcagaa caacgagact cctacaggaa caaaagacgc tactgtgttt     1320

tttttgcatg acaatagact ttataaggct aagggaaaag ctgtgatcat gggaacacct     1380

cagcaggtga atagaaacgt ttgtcttaac cttccaaacc atctttctga ggctatgggt     1440

gacttccatc acgctccaat cttggtggtg aacgtggctc ttaggaactg gaaatctatg     1500

gaaaaggcag gagtgtcagg acttaggtgg tttggagagt accctggtat cggacagatc     1560

gttaggtcta tggtgattga tggaaaggag attatgccat gcgacccttc taagccagct     1620

gttatgacat tttacattcc tatgaatcaa gctactaggg gaatgccaag aggagagcag     1680

gctatgactg ctaggcacat gcttttcaat cttactttcg ctgatattga gttgcttatc     1740

agggaccagt tgactagggc tttcggttct tacggtttcg acgctaagag ggacattgct     1800

gctatcgtgg ctaacagatg gggtcatgct ttggtttgcg ctggtcctgg attctacact     1860

ggacttaacg gaaagcctcc agtgtcaaag gtgatcacag ctggatggga cagagtggct     1920

tttggacatt cagacttgtc aggaaggcag gcttggacag ttgctgtgaa ctacgctagg     1980

acagcagtgg ctaacgtttt ccctaagata tga                                  2013


<210>  4
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  4
ccgatctatg acgggatatc tggga                                             25


<210>  5
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  5
tgacgcgaca atccctctac c                                                 21


<210>  6
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  6
caccatatga ctgaaaaaac gcctaaacta g                                      31


<210>  7
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  7
gaggaattct cagattttag ggaagacatt cgcaacc                                37


<210>  8
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  8
atggtaccta gggtacgtaa gtacgtactc aa                                     32


<210>  9
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  9
cactcacgga ccgaagtcat gaagaacctg ataagac                                37


