                         SEQUENCE LISTING

<110>  Macquarie University
 
<120>  Method of producing chemicals

<130>  M50195703

<150>  2017900129
<151>  2017-01-17

<160>  39    

<170>  PatentIn version 3.5

<210>  1
<211>  675
<212>  PRT
<213>  Pichia pastoris

<400>  1

Met Ala Ile Pro Glu Glu Phe Asp Ile Leu Val Leu Gly Gly Gly Ser 
1               5                   10                  15      


Ser Gly Ser Cys Ile Ala Gly Arg Leu Ala Asn Leu Asp His Ser Leu 
            20                  25                  30          


Lys Val Gly Leu Ile Glu Ala Gly Glu Asn Asn Leu Asn Asn Pro Trp 
        35                  40                  45              


Val Tyr Leu Pro Gly Ile Tyr Pro Arg Asn Met Lys Leu Asp Ser Lys 
    50                  55                  60                  


Thr Ala Ser Phe Tyr Thr Ser Asn Pro Ser Pro His Leu Asn Gly Arg 
65                  70                  75                  80  


Arg Ala Ile Val Pro Cys Ala Asn Val Leu Gly Gly Gly Ser Ser Ile 
                85                  90                  95      


Asn Phe Met Met Tyr Thr Arg Gly Ser Ala Ser Asp Tyr Asp Asp Phe 
            100                 105                 110         


Gln Ala Glu Gly Trp Lys Thr Lys Asp Leu Leu Pro Leu Met Lys Lys 
        115                 120                 125             


Thr Glu Thr Tyr Gln Arg Ala Cys Asn Asn Pro Asp Ile His Gly Phe 
    130                 135                 140                 


Glu Gly Pro Ile Lys Val Ser Phe Gly Asn Tyr Thr Tyr Pro Val Cys 
145                 150                 155                 160 


Gln Asp Phe Leu Arg Ala Ser Glu Ser Gln Gly Ile Pro Tyr Val Asp 
                165                 170                 175     


Asp Leu Glu Asp Leu Val Thr Ala His Gly Ala Glu His Trp Leu Lys 
            180                 185                 190         


Trp Ile Asn Arg Asp Thr Gly Arg Arg Ser Asp Ser Ala His Ala Phe 
        195                 200                 205             


Val His Ser Thr Met Arg Asn His Asp Asn Leu Tyr Leu Ile Cys Asn 
    210                 215                 220                 


Thr Lys Val Asp Lys Ile Ile Val Glu Asp Gly Arg Ala Ala Ala Val 
225                 230                 235                 240 


Arg Thr Val Pro Ser Lys Pro Leu Asn Pro Lys Lys Pro Ser His Lys 
                245                 250                 255     


Ile Tyr Arg Ala Arg Lys Gln Ile Val Leu Ser Cys Gly Thr Ile Ser 
            260                 265                 270         


Ser Pro Leu Val Leu Gln Arg Ser Gly Phe Gly Asp Pro Ile Lys Leu 
        275                 280                 285             


Arg Ala Ala Gly Val Lys Pro Leu Val Asn Leu Pro Gly Val Gly Arg 
    290                 295                 300                 


Asn Phe Gln Asp His Tyr Cys Phe Phe Ser Pro Tyr Arg Ile Lys Pro 
305                 310                 315                 320 


Gln Tyr Glu Ser Phe Asp Asp Phe Val Arg Gly Asp Ala Glu Ile Gln 
                325                 330                 335     


Lys Arg Val Phe Asp Gln Trp Tyr Ala Asn Gly Thr Gly Pro Leu Ala 
            340                 345                 350         


Thr Asn Gly Ile Glu Ala Gly Val Lys Ile Arg Pro Thr Pro Glu Glu 
        355                 360                 365             


Leu Ser Gln Met Asp Glu Ser Phe Gln Glu Gly Tyr Arg Glu Tyr Phe 
    370                 375                 380                 


Glu Asp Lys Pro Asp Lys Pro Val Met His Tyr Ser Ile Ile Ala Gly 
385                 390                 395                 400 


Phe Phe Gly Asp His Thr Lys Ile Pro Pro Gly Lys Tyr Met Thr Met 
                405                 410                 415     


Phe His Phe Leu Glu Tyr Pro Phe Ser Arg Gly Ser Ile His Ile Thr 
            420                 425                 430         


Ser Pro Asp Pro Tyr Ala Ala Pro Asp Phe Asp Pro Gly Phe Met Asn 
        435                 440                 445             


Asp Glu Arg Asp Met Ala Pro Met Val Trp Ala Tyr Lys Lys Ser Arg 
    450                 455                 460                 


Glu Thr Ala Arg Arg Met Asp His Phe Ala Gly Glu Val Thr Ser His 
465                 470                 475                 480 


His Pro Leu Phe Pro Tyr Ser Ser Glu Ala Arg Ala Leu Glu Met Asp 
                485                 490                 495     


Leu Glu Thr Ser Asn Ala Tyr Gly Gly Pro Leu Asn Leu Ser Ala Gly 
            500                 505                 510         


Leu Ala His Gly Ser Trp Thr Gln Pro Leu Lys Lys Pro Thr Ala Lys 
        515                 520                 525             


Asn Glu Gly His Val Thr Ser Asn Gln Val Glu Leu His Pro Asp Ile 
    530                 535                 540                 


Glu Tyr Asp Glu Glu Asp Asp Lys Ala Ile Glu Asn Tyr Ile Arg Glu 
545                 550                 555                 560 


His Thr Glu Thr Thr Trp His Cys Leu Gly Thr Cys Ser Ile Gly Pro 
                565                 570                 575     


Arg Glu Gly Ser Lys Ile Val Lys Trp Gly Gly Val Leu Asp His Arg 
            580                 585                 590         


Ser Asn Val Tyr Gly Val Lys Gly Leu Lys Val Gly Asp Leu Ser Val 
        595                 600                 605             


Cys Pro Asp Asn Val Gly Cys Asn Thr Tyr Thr Thr Ala Leu Leu Ile 
    610                 615                 620                 


Gly Glu Lys Thr Ala Thr Leu Val Gly Glu Asp Leu Gly Tyr Ser Gly 
625                 630                 635                 640 


Glu Ala Leu Asp Met Thr Val Pro Gln Phe Lys Leu Gly Thr Tyr Glu 
                645                 650                 655     


Lys Thr Gly Leu Ala Arg Phe Pro Glu Ala Leu Ile Lys Ser Met Thr 
            660                 665                 670         


Ser Lys Leu 
        675 


<210>  2
<211>  2028
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised AOX1 DNA sequence

<400>  2
atggctattc cagaagaatt cgacattttg gttttgggtg gtggttcttc tggttcttgt       60

attgctggta gattggctaa cttggaccac tctttgaagg ttggtttgat tgaagctggt      120

gaaaacaact tgaacaaccc atgggtttac ttgccaggta tttacccaag aaacatgaag      180

ttggactcta agactgcttc tttctacact tctaacccat ctccacactt gaacggtaga      240

agagctattg ttccatgtgc taacgttttg ggtggtggtt cttctattaa cttcatgatg      300

tacactagag gttctgcttc tgactacgac gacttccaag ctgaaggttg gaagactaag      360

gacttgttgc cattgatgaa gaagactgaa acttaccaaa gagcttgtaa caacccagac      420

attcacggtt tcgaaggtcc aattaaggtt tctttcggta actacactta cccagtttgt      480

caagacttct tgagagcttc tgaatctcaa ggtattccat acgttgacga cttggaagac      540

ttggttactg ctcacggtgc tgaacactgg ttgaagtgga ttaacagaga cactggtaga      600

agatctgact ctgctcacgc tttcgttcac tctactatga gaaaccacga caacttgtac      660

ttgatttgta acactaaggt tgacaagatt attgttgaag acggtagagc tgctgctgtt      720

agaactgttc catctaagcc attgaaccca aagaagccat ctcacaagat ttacagagct      780

agaaagcaaa ttgttttgtc ttgtggtact atttcttctc cattggtttt gcaaagatct      840

ggtttcggtg acccaattaa gttgagagct gctggtgtta agccattggt taacttgcca      900

ggtgttggta gaaacttcca agaccactac tgtttcttct ctccatacag aattaagcca      960

caatacgaat ctttcgacga cttcgttaga ggtgacgctg aaattcaaaa gagagttttc     1020

gaccaatggt acgctaacgg tactggtcca ttggctacta acggtattga agctggtgtt     1080

aagattagac caactccaga agaattgtct caaatggacg aatctttcca agaaggttac     1140

agagaatact tcgaagacaa gccagacaag ccagttatgc actactctat tattgctggt     1200

ttcttcggtg accacactaa gattccacca ggtaagtaca tgactatgtt ccacttcttg     1260

gaatacccat tctctagagg ttctattcac attacttctc cagacccata cgctgctcca     1320

gacttcgacc caggtttcat gaacgacgaa agagacatgg ctccaatggt ttgggcttac     1380

aagaagtcta gagaaactgc tagaagaatg gaccacttcg ctggtgaagt tacttctcac     1440

cacccattgt tcccatactc ttctgaagct agagctttgg aaatggactt ggaaacttct     1500

aacgcttacg gtggtccatt gaacttgtct gctggtttgg ctcacggttc ttggactcaa     1560

ccattgaaga agccaactgc taagaacgaa ggtcacgtta cttctaacca agttgaattg     1620

cacccagaca ttgaatacga cgaagaagac gacaaggcta ttgaaaacta cattagagaa     1680

cacactgaaa ctacttggca ctgtttgggt acttgttcta ttggtccaag agaaggttct     1740

aagattgtta agtggggtgg tgttttggac cacagatcta acgtttacgg tgttaagggt     1800

ttgaaggttg gtgacttgtc tgtttgtcca gacaacgttg gttgtaacac ttacactact     1860

gctttgttga ttggtgaaaa gactgctact ttggttggtg aagacttggg ttactctggt     1920

gaagctttgg acatgactgt tccacaattc aagttgggta cttacgaaaa gactggtttg     1980

gctagattcc cagaagcttt gattaagtct atgacttcta agttgtaa                  2028


<210>  3
<211>  1189
<212>  PRT
<213>  Pichia pastoris

<400>  3

Met Ala Glu Glu Asp Tyr Leu Pro Val Tyr Gln Leu Arg Arg Asp Ser 
1               5                   10                  15      


Ser Leu Leu Gly Thr Met Asn Lys Ile Leu Val Ala Asn Arg Gly Glu 
            20                  25                  30          


Ile Pro Ile Arg Ile Phe Arg Thr Ala His Glu Leu Ser Met Asn Thr 
        35                  40                  45              


Val Ala Ile Tyr Ser His Glu Asp Arg Leu Ser Met His Arg Leu Lys 
    50                  55                  60                  


Ala Asp Glu Ala Tyr Val Ile Gly Glu Arg Gly Gln Tyr Ser Pro Val 
65                  70                  75                  80  


Gln Ala Tyr Leu Ala Ile Asp Glu Ile Ile Lys Ile Ala Val Lys His 
                85                  90                  95      


Asn Val Asn Met Ile His Pro Gly Tyr Gly Phe Cys Ser Glu Asn Ser 
            100                 105                 110         


Glu Phe Ala Arg Lys Val Glu Glu Asn Gly Ile Leu Trp Val Gly Pro 
        115                 120                 125             


Ser Asp Thr Val Ile Asp Ala Val Gly Asp Lys Val Ser Ala Arg Asn 
    130                 135                 140                 


Leu Ala Tyr Ala Ala Asn Val Pro Thr Val Pro Gly Thr Pro Gly Pro 
145                 150                 155                 160 


Ile Glu Asp Val Ala Gln Ala Thr Ala Phe Val Glu Glu Tyr Gly Tyr 
                165                 170                 175     


Pro Val Ile Ile Lys Ala Ala Phe Gly Gly Gly Gly Arg Gly Met Arg 
            180                 185                 190         


Val Val Arg Glu Gly Asp Asp Ile Glu Asp Ala Phe Leu Arg Ala Ser 
        195                 200                 205             


Ser Glu Ala Lys Thr Ala Phe Gly Asn Gly Thr Val Phe Ile Glu Arg 
    210                 215                 220                 


Phe Leu Asp Lys Pro Lys His Ile Glu Val Gln Leu Leu Ala Asp Asn 
225                 230                 235                 240 


Tyr Gly Asn Val Ile His Leu Phe Glu Arg Asp Cys Ser Val Gln Arg 
                245                 250                 255     


Arg His Gln Lys Val Ala Arg Asn Cys Ser Ala Lys Thr Leu Pro Val 
            260                 265                 270         


Glu Val Arg Asn Ala Ile Leu Asn Asp Ala Val Lys Leu Ala Lys Thr 
        275                 280                 285             


Ala Asn Tyr Arg Asn Ala Gly Thr Ala Glu Phe Leu Val Asp Ser Gln 
    290                 295                 300                 


Asn Arg His Tyr Phe Ile Glu Ile Asn Pro Arg Ile Gln Val Glu His 
305                 310                 315                 320 


Thr Ile Thr Glu Glu Ile Thr Gly Val Asp Ile Val Ala Ala Gln Ile 
                325                 330                 335     


Gln Ile Ala Ala Gly Ala Ser Leu Glu Gln Leu Gly Leu Leu Gln Glu 
            340                 345                 350         


Lys Ile Thr Thr Arg Gly Phe Ala Ile Gln Cys Arg Ile Thr Thr Glu 
        355                 360                 365             


Asp Pro Thr Lys Asn Phe Gln Pro Asp Thr Gly Lys Ile Glu Val Tyr 
    370                 375                 380                 


Arg Ser Ser Gly Gly Asn Gly Val Arg Leu Asp Gly Gly Asn Gly Phe 
385                 390                 395                 400 


Ala Gly Ala Val Ile Ser Pro His Tyr Asp Ser Met Leu Val Lys Cys 
                405                 410                 415     


Ser Thr Ser Gly Ser Asn Tyr Glu Ile Arg Arg Arg Lys Met Ile Arg 
            420                 425                 430         


Ala Leu Val Glu Phe Arg Ile Arg Gly Val Lys Thr Asn Ile Pro Phe 
        435                 440                 445             


Leu Leu Ala Leu Leu Thr His Pro Val Phe Met Thr Ser Glu Cys Trp 
    450                 455                 460                 


Thr Thr Phe Ile Asp Asp Thr Pro Glu Leu Phe Lys Ile Leu Thr Ser 
465                 470                 475                 480 


Gln Asn Arg Ala Gln Lys Leu Leu Ala Tyr Leu Gly Asp Leu Ala Val 
                485                 490                 495     


Asn Gly Ser Ser Ile Lys Gly Gln Ile Gly Leu Pro Lys Leu His Lys 
            500                 505                 510         


Glu Ala Asp Ile Pro Ser Ile Thr Asp Ile Asn Gly Asp Val Ile Asp 
        515                 520                 525             


Val Ser Ile Pro Pro Pro Asp Gly Trp Arg Gln Phe Leu Leu Glu Lys 
    530                 535                 540                 


Gly Pro Glu Gln Phe Ala Gln Gln Val Arg Ala Phe Pro Gly Leu Met 
545                 550                 555                 560 


Ile Met Asp Thr Thr Trp Arg Asp Ala His Gln Ser Leu Leu Ala Thr 
                565                 570                 575     


Arg Val Arg Thr His Asp Leu Leu Asn Ile Ala Pro Ala Thr Ser Tyr 
            580                 585                 590         


Ala Leu His His Ala Phe Ala Leu Glu Cys Trp Gly Gly Ala Thr Phe 
        595                 600                 605             


Asp Val Ser Met Arg Phe Leu His Glu Asp Pro Trp Gln Arg Leu Arg 
    610                 615                 620                 


Lys Leu Arg Lys Ala Val Pro Asn Ile Pro Phe Ser Met Leu Leu Arg 
625                 630                 635                 640 


Gly Gly Asn Gly Val Ala Tyr Tyr Ser Leu Pro Asp Asn Ala Ile Asp 
                645                 650                 655     


His Phe Leu Lys Gln Ala Lys Asp Thr Gly Val Asp Val Phe Arg Val 
            660                 665                 670         


Phe Asp Ala Leu Asn Asp Ile Glu Gln Leu Lys Val Gly Val Asp Ala 
        675                 680                 685             


Val Lys Lys Ala Gly Gly Val Val Glu Ala Thr Met Cys Tyr Ser Gly 
    690                 695                 700                 


Asp Met Leu Lys Pro Gly Lys Lys Tyr Asn Leu Glu Tyr Tyr Ile Asn 
705                 710                 715                 720 


Leu Ala Thr Glu Ile Val Glu Met Gly Thr His Ile Leu Ala Val Lys 
                725                 730                 735     


Asp Met Ala Gly Thr Leu Lys Pro Thr Ala Ala Lys Gln Leu Ile Ser 
            740                 745                 750         


Ala Leu Arg Arg Lys Phe Pro Ser Leu Pro Ile His Val His Thr His 
        755                 760                 765             


Asp Ser Ala Gly Thr Gly Val Ala Ser Met Val Ala Cys Ala Arg Ala 
    770                 775                 780                 


Gly Ala Asp Val Val Thr Val Arg Val Asn Ser Met Ser Gly Met Thr 
785                 790                 795                 800 


Ser Gln Pro Ser Met Ser Ala Phe Ile Ala Ser Leu Asp Gly Glu Ile 
                805                 810                 815     


Glu Thr Gly Ile Pro Glu Ala Asn Ala Arg Glu Ile Asp Ala Tyr Trp 
            820                 825                 830         


Ala Glu Met Arg Leu Leu Tyr Ser Cys Phe Glu Ala Asp Leu Lys Gly 
        835                 840                 845             


Pro Asp Pro Glu Val Tyr Gln His Glu Ile Pro Gly Gly Gln Leu Thr 
    850                 855                 860                 


Asn Leu Leu Phe Gln Ala Gln Gln Val Gly Leu Gly Glu Lys Trp Val 
865                 870                 875                 880 


Glu Thr Lys Lys Ala Tyr Glu Ala Ala Asn Arg Leu Leu Gly Asp Ile 
                885                 890                 895     


Val Lys Val Thr Pro Thr Ser Lys Val Val Gly Asp Leu Ala Gln Phe 
            900                 905                 910         


Met Val Ser Asn Lys Leu Ser Ser Glu Asp Val Glu Arg Leu Ala Ser 
        915                 920                 925             


Glu Leu Asp Phe Pro Asp Ser Val Leu Asp Phe Phe Glu Gly Leu Met 
    930                 935                 940                 


Gly Thr Pro Tyr Gly Gly Phe Pro Glu Pro Leu Arg Thr Asn Val Ile 
945                 950                 955                 960 


Ser Gly Lys Arg Arg Lys Leu Thr Ser Arg Pro Gly Leu Thr Leu Glu 
                965                 970                 975     


Pro Tyr Asn Ile Pro Ala Ile Arg Glu Asp Leu Glu Ala Arg Phe Ser 
            980                 985                 990         


Lys Val Thr Glu Asn Asp Val Ala  Ser Tyr Asn Met Tyr  Pro Lys Val 
        995                 1000                 1005             


Tyr Glu  Ala Tyr Lys Lys Gln  Gln Glu Leu Tyr Gly  Asp Leu Ser 
    1010                 1015                 1020             


Val Leu  Pro Thr Arg Asn Phe  Leu Ser Pro Pro Lys  Ile Asp Glu 
    1025                 1030                 1035             


Glu Arg  His Val Thr Ile Val  Thr Ile Glu Thr Arg  Lys Thr Leu 
    1040                 1045                 1050             


Ile Ile  Lys Cys Met Ala Glu  Gly Glu Leu Ser Gln  Ser Ser Gly 
    1055                 1060                 1065             


Thr Arg  Glu Val Tyr Phe Glu  Leu Asn Gly Glu Met  Arg Lys Val 
    1070                 1075                 1080             


Thr Val  Glu Asp Lys Asn Gly  Ala Val Glu Thr Ile  Thr Arg Pro 
    1085                 1090                 1095             


Lys Ala  Asp Ala His Asn Pro  Asn Glu Ile Gly Ala  Pro Met Ala 
    1100                 1105                 1110             


Gly Val  Val Val Glu Val Arg  Val His Glu Asn Gly  Glu Val Lys 
    1115                 1120                 1125             


Lys Gly  Asp Pro Ile Ala Val  Leu Ser Ala Met Lys  Met Glu Met 
    1130                 1135                 1140             


Val Ile  Ser Ser Pro Val Ala  Gly Arg Ile Gly Gln  Ile Ala Val 
    1145                 1150                 1155             


Lys Glu  Asn Asp Ser Val Asp  Ala Ser Asp Leu Ile  Pro Lys Ser 
    1160                 1165                 1170             


Ser Arg  Leu Ser Lys Leu Leu  Met Phe Ile Ile Leu  Ile Ile Leu 
    1175                 1180                 1185             


Tyr 
    


<210>  4
<211>  3570
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised  PYC1 DNA sequence

<400>  4
atggctgaag aagactactt gccagtttac caattgagaa gagactcttc tttgttgggt       60

actatgaaca agattttggt tgctaacaga ggtgaaattc caattagaat tttcagaact      120

gctcacgaat tgtctatgaa cactgttgct atttactctc acgaagacag attgtctatg      180

cacagattga aggctgacga agcttacgtt attggtgaaa gaggtcaata ctctccagtt      240

caagcttact tggctattga cgaaattatt aagattgctg ttaagcacaa cgttaacatg      300

attcacccag gttacggttt ctgttctgaa aactctgaat tcgctagaaa ggttgaagaa      360

aacggtattt tgtgggttgg tccatctgac actgttattg acgctgttgg tgacaaggtt      420

tctgctagaa acttggctta cgctgctaac gttccaactg ttccaggtac tccaggtcca      480

attgaagacg ttgctcaagc tactgctttc gttgaagaat acggttaccc agttattatt      540

aaggctgctt tcggtggtgg tggtagaggt atgagagttg ttagagaagg tgacgacatt      600

gaagacgctt tcttgagagc ttcttctgaa gctaagactg ctttcggtaa cggtactgtt      660

ttcattgaaa gattcttgga caagccaaag cacattgaag ttcaattgtt ggctgacaac      720

tacggtaacg ttattcactt gttcgaaaga gactgttctg ttcaaagaag acaccaaaag      780

gttgctagaa actgttctgc taagactttg ccagttgaag ttagaaacgc tattttgaac      840

gacgctgtta agttggctaa gactgctaac tacagaaacg ctggtactgc tgaattcttg      900

gttgactctc aaaacagaca ctacttcatt gaaattaacc caagaattca agttgaacac      960

actattactg aagaaattac tggtgttgac attgttgctg ctcaaattca aattgctgct     1020

ggtgcttctt tggaacaatt gggtttgttg caagaaaaga ttactactag aggtttcgct     1080

attcaatgta gaattactac tgaagaccca actaagaact tccaaccaga cactggtaag     1140

attgaagttt acagatcttc tggtggtaac ggtgttagat tggacggtgg taacggtttc     1200

gctggtgctg ttatttctcc acactacgac tctatgttgg ttaagtgttc tacttctggt     1260

tctaactacg aaattagaag aagaaagatg attagagctt tggttgaatt cagaattaga     1320

ggtgttaaga ctaacattcc attcttgttg gctttgttga ctcacccagt tttcatgact     1380

tctgaatgtt ggactacttt cattgacgac actccagaat tgttcaagat tttgacttct     1440

caaaacagag ctcaaaagtt gttggcttac ttgggtgact tggctgttaa cggttcttct     1500

attaagggtc aaattggttt gccaaagttg cacaaggaag ctgacattcc atctattact     1560

gacattaacg gtgacgttat tgacgtttct attccaccac cagacggttg gagacaattc     1620

ttgttggaaa agggtccaga acaattcgct caacaagtta gagctttccc aggtttgatg     1680

attatggaca ctacttggag agacgctcac caatctttgt tggctactag agttagaact     1740

cacgacttgt tgaacattgc tccagctact tcttacgctt tgcaccacgc tttcgctttg     1800

gaatgttggg gtggtgctac tttcgacgtt tctatgagat tcttgcacga agacccatgg     1860

caaagattga gaaagttgag aaaggctgtt ccaaacattc cattctctat gttgttgaga     1920

ggtggtaacg gtgttgctta ctactctttg ccagacaacg ctattgacca cttcttgaag     1980

caagctaagg acactggtgt tgacgttttc agagttttcg acgctttgaa cgacattgaa     2040

caattgaagg ttggtgttga cgctgttaag aaggctggtg gtgttgttga agctactatg     2100

tgttactctg gtgacatgtt gaagccaggt aagaagtaca acttggaata ctacattaac     2160

ttggctactg aaattgttga aatgggtact cacattttgg ctgttaagga catggctggt     2220

actttgaagc caactgctgc taagcaattg atttctgctt tgagaagaaa gttcccatct     2280

ttgccaattc acgttcacac tcacgactct gctggtactg gtgttgcttc tatggttgct     2340

tgtgctagag ctggtgctga cgttgttact gttagagtta actctatgtc tggtatgact     2400

tctcaaccat ctatgtctgc tttcattgct tctttggacg gtgaaattga aactggtatt     2460

ccagaagcta acgctagaga aattgacgct tactgggctg aaatgagatt gttgtactct     2520

tgtttcgaag ctgacttgaa gggtccagac ccagaagttt accaacacga aattccaggt     2580

ggtcaattga ctaacttgtt gttccaagct caacaagttg gtttgggtga aaagtgggtt     2640

gaaactaaga aggcttacga agctgctaac agattgttgg gtgacattgt taaggttact     2700

ccaacttcta aggttgttgg tgacttggct caattcatgg tttctaacaa gttgtcttct     2760

gaagacgttg aaagattggc ttctgaattg gacttcccag actctgtttt ggacttcttc     2820

gaaggtttga tgggtactcc atacggtggt ttcccagaac cattgagaac taacgttatt     2880

tctggtaaga gaagaaagtt gacttctaga ccaggtttga ctttggaacc atacaacatt     2940

ccagctatta gagaagactt ggaagctaga ttctctaagg ttactgaaaa cgacgttgct     3000

tcttacaaca tgtacccaaa ggtttacgaa gcttacaaga agcaacaaga attgtacggt     3060

gacttgtctg ttttgccaac tagaaacttc ttgtctccac caaagattga cgaagaaaga     3120

cacgttacta ttgttactat tgaaactaga aagactttga ttattaagtg tatggctgaa     3180

ggtgaattgt ctcaatcttc tggtactaga gaagtttact tcgaattgaa cggtgaaatg     3240

agaaaggtta ctgttgaaga caagaacggt gctgttgaaa ctattactag accaaaggct     3300

gacgctcaca acccaaacga aattggtgct ccaatggctg gtgttgttgt tgaagttaga     3360

gttcacgaaa acggtgaagt taagaagggt gacccaattg ctgttttgtc tgctatgaag     3420

atggaaatgg ttatttcttc tccagttgct ggtagaattg gtcaaattgc tgttaaggaa     3480

aacgactctg ttgacgcttc tgacttgatt ccaaagtctt ctagattgtc taagttgttg     3540

atgttcatta ttttgattat tttgtactaa                                      3570


<210>  5
<211>  719
<212>  PRT
<213>  Pichia pastoris

<400>  5

Met Ala Arg Ile Pro Lys Ala Val Ser Thr Gln Asp Asp Ile His Glu 
1               5                   10                  15      


Leu Val Ile Lys Thr Phe Arg Cys Tyr Val Leu Asp Leu Val Glu Gln 
            20                  25                  30          


Tyr Gly Gly Gly His Pro Gly Ser Ala Met Gly Met Val Ala Ile Gly 
        35                  40                  45              


Ile Ala Leu Trp Lys Tyr Gln Met Lys Tyr Ala Pro Asn Asp Pro Asp 
    50                  55                  60                  


Tyr Phe Asn Arg Asp Arg Phe Val Leu Ser Asn Gly His Val Cys Leu 
65                  70                  75                  80  


Phe Gln Tyr Leu Phe Gln His Leu Thr Gly Leu Lys Glu Met Thr Val 
                85                  90                  95      


Lys Gln Leu Gln Ser Tyr His Ser Ser Asp Tyr His Ser Leu Thr Pro 
            100                 105                 110         


Gly His Pro Glu Ile Glu Asn Pro Ala Val Glu Val Thr Thr Gly Pro 
        115                 120                 125             


Leu Gly Gln Gly Ile Ser Asn Ala Val Gly Met Ala Ile Gly Ser Lys 
    130                 135                 140                 


Asn Leu Ala Ala Thr Tyr Asn Arg Pro Gly Phe Pro Val Val Asp Asn 
145                 150                 155                 160 


Thr Ile Tyr Ala Ile Val Gly Asp Ala Cys Leu Gln Glu Gly Pro Ala 
                165                 170                 175     


Leu Glu Ser Ile Ser Leu Ala Gly His Leu Ala Leu Asp Asn Leu Ile 
            180                 185                 190         


Val Ile Tyr Asp Asn Asn Gln Val Cys Cys Asp Gly Ser Val Asp Val 
        195                 200                 205             


Asn Asn Thr Glu Asp Ile Ser Ala Lys Phe Arg Ala Gln Asn Trp Asn 
    210                 215                 220                 


Val Ile Asp Ile Val Asp Gly Ser Arg Asp Val Ala Thr Ile Val Lys 
225                 230                 235                 240 


Ala Ile Asp Trp Ala Lys Ala Glu Thr Glu Arg Pro Thr Leu Ile Asn 
                245                 250                 255     


Val Arg Thr Glu Ile Gly Gln Asp Ser Ala Phe Gly Asn His His Ala 
            260                 265                 270         


Ala His Gly Ser Ala Leu Gly Glu Glu Gly Ile Arg Glu Leu Lys Thr 
        275                 280                 285             


Lys Tyr Gly Phe Asn Pro Ala Gln Lys Phe Trp Phe Pro Lys Glu Val 
    290                 295                 300                 


Tyr Asp Phe Phe Ala Glu Lys Pro Ala Lys Gly Asp Glu Leu Val Lys 
305                 310                 315                 320 


Asn Trp Lys Lys Leu Val Asp Ser Tyr Val Lys Glu Tyr Pro Arg Glu 
                325                 330                 335     


Gly Gln Glu Phe Leu Ser Arg Val Arg Gly Glu Leu Pro Lys Asn Trp 
            340                 345                 350         


Arg Thr Tyr Ile Pro Gln Asp Lys Pro Thr Glu Pro Thr Ala Thr Arg 
        355                 360                 365             


Thr Ser Ala Arg Glu Ile Val Arg Ala Leu Gly Lys Asn Leu Pro Gln 
    370                 375                 380                 


Val Ile Ala Gly Ser Gly Asp Leu Ser Val Ser Ile Leu Leu Asn Trp 
385                 390                 395                 400 


Asp Gly Val Lys Tyr Phe Phe Asn Pro Lys Leu Gln Thr Phe Cys Gly 
                405                 410                 415     


Leu Gly Gly Asp Tyr Ser Gly Arg Tyr Ile Glu Phe Gly Ile Arg Glu 
            420                 425                 430         


His Ser Met Cys Ala Ile Ala Asn Gly Leu Ala Ala Tyr Asn Lys Gly 
        435                 440                 445             


Thr Phe Leu Pro Ile Thr Ser Thr Phe Tyr Met Phe Tyr Leu Tyr Ala 
    450                 455                 460                 


Ala Pro Ala Leu Arg Met Ala Ala Leu Gln Glu Leu Lys Ala Ile His 
465                 470                 475                 480 


Ile Ala Thr His Asp Ser Ile Gly Ala Gly Glu Asp Gly Pro Thr His 
                485                 490                 495     


Gln Pro Ile Ala Leu Ser Ser Leu Phe Arg Ala Met Pro Asn Phe Tyr 
            500                 505                 510         


Tyr Met Arg Pro Ala Asp Ala Thr Glu Val Ala Ala Leu Phe Glu Val 
        515                 520                 525             


Ala Val Glu Leu Glu His Ser Thr Leu Leu Ser Leu Ser Arg His Glu 
    530                 535                 540                 


Val Asp Gln Tyr Pro Gly Lys Thr Ser Ala Gln Gly Ala Lys Arg Gly 
545                 550                 555                 560 


Gly Tyr Val Val Glu Asp Cys Glu Gly Lys Pro Asp Val Gln Leu Ile 
                565                 570                 575     


Gly Thr Gly Ser Glu Leu Glu Phe Ala Ile Lys Thr Ala Arg Leu Leu 
            580                 585                 590         


Arg Gln Gln Lys Gly Trp Lys Val Arg Val Leu Ser Phe Pro Cys Gln 
        595                 600                 605             


Arg Leu Phe Asp Glu Gln Ser Ile Thr Tyr Arg Arg Ser Val Leu Arg 
    610                 615                 620                 


Arg Gly Glu Val Pro Thr Val Val Val Glu Ala Tyr Val Ala Tyr Gly 
625                 630                 635                 640 


Trp Glu Arg Tyr Ala Thr Ala Gly Tyr Thr Met Asn Thr Phe Gly Lys 
                645                 650                 655     


Ser Leu Pro Val Glu Asp Val Tyr Lys Tyr Phe Gly Tyr Thr Pro Glu 
            660                 665                 670         


Lys Ile Gly Glu Arg Val Val Gln Tyr Val Asn Ser Ile Lys Ala Ser 
        675                 680                 685             


Pro Gln Ile Leu Tyr Glu Phe His Asp Leu Lys Gly Lys Pro Lys His 
    690                 695                 700                 


Asp Lys Leu Pro Glu Ala Leu Ile Lys Ser Met Thr Ser Lys Leu 
705                 710                 715                 


<210>  6
<211>  2160
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised DAS DNA sequence

<400>  6
atggctagaa ttccaaaggc tgtttctact caagacgaca ttcacgaatt ggttattaag       60

actttcagat gttacgtttt ggacttggtt gaacaatacg gtggtggtca cccaggttct      120

gctatgggta tggttgctat tggtattgct ttgtggaagt accaaatgaa gtacgctcca      180

aacgacccag actacttcaa cagagacaga ttcgttttgt ctaacggtca cgtttgtttg      240

ttccaatact tgttccaaca cttgactggt ttgaaggaaa tgactgttaa gcaattgcaa      300

tcttaccact cttctgacta ccactctttg actccaggtc acccagaaat tgaaaaccca      360

gctgttgaag ttactactgg tccattgggt caaggtattt ctaacgctgt tggtatggct      420

attggttcta agaacttggc tgctacttac aacagaccag gtttcccagt tgttgacaac      480

actatttacg ctattgttgg tgacgcttgt ttgcaagaag gtccagcttt ggaatctatt      540

tctttggctg gtcacttggc tttggacaac ttgattgtta tttacgacaa caaccaagtt      600

tgttgtgacg gttctgttga cgttaacaac actgaagaca tttctgctaa gttcagagct      660

caaaactgga acgttattga cattgttgac ggttctagag acgttgctac tattgttaag      720

gctattgact gggctaaggc tgaaactgaa agaccaactt tgattaacgt tagaactgaa      780

attggtcaag actctgcttt cggtaaccac cacgctgctc acggttctgc tttgggtgaa      840

gaaggtatta gagaattgaa gactaagtac ggtttcaacc cagctcaaaa gttctggttc      900

ccaaaggaag tttacgactt cttcgctgaa aagccagcta agggtgacga attggttaag      960

aactggaaga agttggttga ctcttacgtt aaggaatacc caagagaagg tcaagaattc     1020

ttgtctagag ttagaggtga attgccaaag aactggagaa cttacattcc acaagacaag     1080

ccaactgaac caactgctac tagaacttct gctagagaaa ttgttagagc tttgggtaag     1140

aacttgccac aagttattgc tggttctggt gacttgtctg tttctatttt gttgaactgg     1200

gacggtgtta agtacttctt caacccaaag ttgcaaactt tctgtggttt gggtggtgac     1260

tactctggta gatacattga attcggtatt agagaacact ctatgtgtgc tattgctaac     1320

ggtttggctg cttacaacaa gggtactttc ttgccaatta cttctacttt ctacatgttc     1380

tacttgtacg ctgctccagc tttgagaatg gctgctttgc aagaattgaa ggctattcac     1440

attgctactc acgactctat tggtgctggt gaagacggtc caactcacca accaattgct     1500

ttgtcttctt tgttcagagc tatgccaaac ttctactaca tgagaccagc tgacgctact     1560

gaagttgctg ctttgttcga agttgctgtt gaattggaac actctacttt gttgtctttg     1620

tctagacacg aagttgacca atacccaggt aagacttctg ctcaaggtgc taagagaggt     1680

ggttacgttg ttgaagactg tgaaggtaag ccagacgttc aattgattgg tactggttct     1740

gaattggaat tcgctattaa gactgctaga ttgttgagac aacaaaaggg ttggaaggtt     1800

agagttttgt ctttcccatg tcaaagattg ttcgacgaac aatctattac ttacagaaga     1860

tctgttttga gaagaggtga agttccaact gttgttgttg aagcttacgt tgcttacggt     1920

tgggaaagat acgctactgc tggttacact atgaacactt tcggtaagtc tttgccagtt     1980

gaagacgttt acaagtactt cggttacact ccagaaaaga ttggtgaaag agttgttcaa     2040

tacgttaact ctattaaggc ttctccacaa attttgtacg aattccacga cttgaagggt     2100

aagccaaagc acgacaagtt gccagaagct ttgattaagt ctatgacttc taagttgtaa     2160


<210>  7
<211>  596
<212>  PRT
<213>  Pichia pastoris

<400>  7

Met Ser Ala Lys Ser Phe Glu Val Thr Asp Pro Val Asn Ser Ser Leu 
1               5                   10                  15      


Lys Gly Phe Ala Leu Ala Asn Pro Ser Ile Thr Leu Val Pro Glu Glu 
            20                  25                  30          


Lys Ile Leu Phe Arg Lys Thr Asp Ser Asp Lys Ile Ala Leu Ile Ser 
        35                  40                  45              


Gly Gly Gly Ser Gly His Glu Pro Thr His Ala Gly Phe Ile Gly Lys 
    50                  55                  60                  


Gly Met Leu Ser Gly Ala Val Val Gly Glu Ile Phe Ala Ser Pro Ser 
65                  70                  75                  80  


Thr Lys Gln Ile Leu Asn Ala Ile Arg Leu Val Asn Glu Asn Ala Ser 
                85                  90                  95      


Gly Val Leu Leu Ile Val Lys Asn Tyr Thr Gly Asp Val Leu His Phe 
            100                 105                 110         


Gly Leu Ser Ala Glu Arg Ala Arg Ala Leu Gly Ile Asn Cys Arg Val 
        115                 120                 125             


Ala Val Ile Gly Asp Asp Val Ala Val Gly Arg Glu Lys Gly Gly Met 
    130                 135                 140                 


Val Gly Arg Arg Ala Leu Ala Gly Thr Val Leu Val His Lys Ile Val 
145                 150                 155                 160 


Gly Ala Phe Ala Glu Glu Tyr Ser Ser Lys Tyr Gly Leu Asp Gly Thr 
                165                 170                 175     


Ala Lys Val Ala Lys Ile Ile Asn Asp Asn Leu Val Thr Ile Gly Ser 
            180                 185                 190         


Ser Leu Asp His Cys Lys Val Pro Gly Arg Lys Phe Glu Ser Glu Leu 
        195                 200                 205             


Asn Glu Lys Gln Met Glu Leu Gly Met Gly Ile His Asn Glu Pro Gly 
    210                 215                 220                 


Val Lys Val Leu Asp Pro Ile Pro Ser Thr Glu Asp Leu Ile Ser Lys 
225                 230                 235                 240 


Tyr Met Leu Pro Lys Leu Leu Asp Pro Asn Asp Lys Asp Arg Ala Phe 
                245                 250                 255     


Val Lys Phe Asp Glu Asp Asp Glu Val Val Leu Leu Val Asn Asn Leu 
            260                 265                 270         


Gly Gly Val Ser Asn Phe Val Ile Ser Ser Ile Thr Ser Lys Thr Thr 
        275                 280                 285             


Asp Phe Leu Lys Glu Asn Tyr Asn Ile Thr Pro Val Gln Thr Ile Ala 
    290                 295                 300                 


Gly Thr Leu Met Thr Ser Phe Asn Gly Asn Gly Phe Ser Ile Thr Leu 
305                 310                 315                 320 


Leu Asn Ala Thr Lys Ala Thr Lys Ala Leu Gln Ser Asp Phe Glu Glu 
                325                 330                 335     


Ile Lys Ser Val Leu Asp Leu Leu Asn Ala Phe Thr Asn Ala Pro Gly 
            340                 345                 350         


Trp Pro Ile Ala Asp Phe Glu Lys Thr Ser Ala Pro Ser Val Asn Asp 
        355                 360                 365             


Asp Leu Leu His Asn Glu Val Thr Ala Lys Ala Val Gly Thr Tyr Asp 
    370                 375                 380                 


Phe Asp Lys Phe Ala Glu Trp Met Lys Ser Gly Ala Glu Gln Val Ile 
385                 390                 395                 400 


Lys Ser Glu Pro His Ile Thr Glu Leu Asp Asn Gln Val Gly Asp Gly 
                405                 410                 415     


Asp Cys Gly Tyr Thr Leu Val Ala Gly Val Lys Gly Ile Thr Glu Asn 
            420                 425                 430         


Leu Asp Lys Leu Ser Lys Asp Ser Leu Ser Gln Ala Val Ala Gln Ile 
        435                 440                 445             


Ser Asp Phe Ile Glu Gly Ser Met Gly Gly Thr Ser Gly Gly Leu Tyr 
    450                 455                 460                 


Ser Ile Leu Leu Ser Gly Phe Ser His Gly Leu Ile Gln Val Cys Lys 
465                 470                 475                 480 


Ser Lys Asp Glu Pro Val Thr Lys Glu Ile Val Ala Lys Ser Leu Gly 
                485                 490                 495     


Ile Ala Leu Asp Thr Leu Tyr Lys Tyr Thr Lys Ala Arg Lys Gly Ser 
            500                 505                 510         


Ser Thr Met Ile Asp Ala Leu Glu Pro Phe Val Lys Glu Phe Thr Ala 
        515                 520                 525             


Ser Lys Asp Phe Asn Lys Ala Val Lys Ala Ala Glu Glu Gly Ala Lys 
    530                 535                 540                 


Ser Thr Ala Thr Phe Glu Ala Lys Phe Gly Arg Ala Ser Tyr Val Gly 
545                 550                 555                 560 


Asp Ser Ser Gln Val Glu Asp Pro Gly Ala Val Gly Leu Cys Glu Phe 
                565                 570                 575     


Leu Lys Gly Val Gln Ser Ala Leu Pro Glu Ala Leu Ile Lys Ser Met 
            580                 585                 590         


Thr Ser Lys Leu 
        595     


<210>  8
<211>  1791
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised DAK DNA sequence

<400>  8
atgtccgcta aatcgtttga agtcacagat ccagtcaatt caagtctcaa agggtttgcc       60

cttgctaacc cctccattac gctggtccct gaagaaaaaa ttctcttcag aaagaccgat      120

tccgacaaga tcgcattaat ttctggtggt ggtagtggac atgaacctac acacgccggt      180

ttcattggta agggtatgtt gagtggcgcc gtggttggcg aaatttttgc atccccttca      240

acaaaacaga ttttaaatgc aatccgttta gtcaatgaaa atgcgtctgg cgttttattg      300

attgtgaaga actacacagg tgatgttttg cattttggtc tgtccgctga gagagcaaga      360

gccttgggta ttaactgccg cgttgctgtc ataggtgatg atgttgcagt tggcagagaa      420

aagggtggta tggttggtag aagagcattg gcaggtaccg ttttggttca taagattgta      480

ggtgccttcg cagaagaata ttctagtaag tatggcttag acggtacagc taaagtggct      540

aaaattatca acgacaattt ggtgaccatt ggatcttctt tagaccattg taaagttcct      600

ggcaggaaat tcgaaagtga attaaacgaa aaacaaatgg aattgggtat gggtattcat      660

aacgaacctg gtgtgaaagt tttagaccct attccttcta ccgaagactt gatctccaag      720

tatatgctac caaaactatt ggatccaaac gataaggata gagcttttgt aaagtttgat      780

gaagatgatg aagttgtctt gttagttaac aatctcggcg gtgtttctaa ttttgttatt      840

agttctatca cttccaaaac tacggatttc ttaaaggaaa attacaacat aaccccggtt      900

caaacaattg ctggcacatt gatgacctcc ttcaatggta atgggttcag tatcacatta      960

ctaaacgcca ctaaggctac aaaggctttg caatctgatt ttgaggagat caaatcagta     1020

ctagacttgt tgaacgcatt tacgaacgca ccgggctggc caattgcaga ttttgaaaag     1080

acttctgccc catctgttaa cgatgacttg ttacataatg aagtaacagc aaaggccgtc     1140

ggtacctatg actttgacaa gtttgctgag tggatgaaga gtggtgctga acaagttatc     1200

aagagcgaac cgcacattac ggaactagac aatcaagttg gtgatggtga ttgtggttac     1260

actttagtgg caggagttaa aggcatcacc gaaaaccttg acaagctgtc gaaggactca     1320

ttatctcagg cggttgccca aatttcagat ttcattgaag gctcaatggg aggtacttct     1380

ggtggtttat attctattct tttgtcgggt ttttcacacg gattaattca ggtttgtaaa     1440

tcaaaggatg aacccgtcac taaggaaatt gtggctaagt cactcggaat tgcattggat     1500

actttataca aatatacaaa ggcaaggaag ggatcatcca ccatgattga tgctttagaa     1560

ccattcgtta aagaatttac tgcatctaag gatttcaata aggcggtaaa agctgcagag     1620

gaaggtgcta aatccactgc tacattcgag gccaaatttg gcagagcttc gtatgtcggc     1680

gattcatctc aagtagaaga tcctggtgca gtaggcctat gtgagttttt gaagggggtt     1740

caaagcgcct tgccagaagc tttgattaag tctatgactt ctaagttgta a              1791


<210>  9
<211>  371
<212>  PRT
<213>  Pichia pastoris

<400>  9

Met Gly Val Glu Gln Ile Leu Lys Arg Lys Thr Gly Val Ile Val Gly 
1               5                   10                  15      


Glu Asp Val His Asn Leu Phe Thr Tyr Ala Lys Glu His Lys Phe Ala 
            20                  25                  30          


Ile Pro Ala Ile Asn Val Thr Ser Ser Ser Thr Ala Val Ala Ala Leu 
        35                  40                  45              


Glu Ala Ala Arg Asp Ser Lys Ser Pro Ile Ile Leu Gln Thr Ser Asn 
    50                  55                  60                  


Gly Gly Ala Ala Tyr Phe Ala Gly Lys Gly Ile Ser Asn Glu Gly Gln 
65                  70                  75                  80  


Asn Ala Ser Ile Lys Gly Ala Ile Ala Ala Ala His Tyr Ile Arg Ser 
                85                  90                  95      


Ile Ala Pro Ala Tyr Gly Ile Pro Val Val Leu His Ser Asp His Cys 
            100                 105                 110         


Ala Lys Lys Leu Leu Pro Trp Phe Asp Gly Met Leu Glu Ala Asp Glu 
        115                 120                 125             


Ala Tyr Phe Lys Glu His Gly Glu Pro Leu Phe Ser Ser His Met Leu 
    130                 135                 140                 


Asp Leu Ser Glu Glu Thr Asp Glu Glu Asn Ile Ser Thr Cys Val Lys 
145                 150                 155                 160 


Tyr Phe Lys Arg Met Ala Ala Met Asp Gln Trp Leu Glu Met Glu Ile 
                165                 170                 175     


Gly Ile Thr Gly Gly Glu Glu Asp Gly Val Asn Asn Glu Asn Ala Asp 
            180                 185                 190         


Lys Glu Asp Leu Tyr Thr Lys Pro Glu Gln Val Tyr Asn Val Tyr Lys 
        195                 200                 205             


Ala Leu His Pro Ile Ser Pro Asn Phe Ser Ile Ala Ala Ala Phe Gly 
    210                 215                 220                 


Asn Cys His Gly Leu Tyr Ala Gly Asp Ile Ala Leu Arg Pro Glu Ile 
225                 230                 235                 240 


Leu Ala Glu His Gln Lys Tyr Thr Arg Glu Gln Val Gly Cys Lys Glu 
                245                 250                 255     


Glu Lys Pro Leu Phe Leu Val Phe His Gly Gly Ser Gly Ser Thr Val 
            260                 265                 270         


Gln Glu Phe His Thr Gly Ile Asp Asn Gly Val Val Lys Val Asn Leu 
        275                 280                 285             


Asp Thr Asp Cys Gln Tyr Ala Tyr Leu Thr Gly Ile Arg Asp Tyr Val 
    290                 295                 300                 


Leu Asn Lys Lys Asp Tyr Ile Met Ser Pro Val Gly Asn Pro Glu Gly 
305                 310                 315                 320 


Pro Glu Lys Pro Asn Lys Lys Phe Phe Asp Pro Arg Val Trp Val Arg 
                325                 330                 335     


Glu Gly Glu Lys Thr Met Gly Ala Lys Ile Thr Lys Ser Leu Glu Thr 
            340                 345                 350         


Phe Arg Thr Thr Asn Thr Leu Pro Glu Ala Leu Ile Lys Ser Met Thr 
        355                 360                 365             


Ser Lys Leu 
    370     


<210>  10
<211>  1116
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised FBA1-2 DNA sequence

<400>  10
atgggtgttg aacaaatctt aaagagaaag accggtgtca tcgttggtga agatgtccac       60

aacttattca cttacgctaa ggaacacaag ttcgctattc cagctattaa cgtcacctct      120

tcttctactg ccgtcgctgc tttagaagct gctagagaca gcaagtcccc aatcattttg      180

caaacctcta acggtggtgc tgcttacttc gctggtaagg gtatctctaa cgaaggtcaa      240

aatgcttcca tcaagggtgc tattgccgct gcccactaca tcagatccat tgctccagct      300

tacggtatcc cagttgtctt acactctgac cactgtgcca agaagttgtt gccatggttc      360

gatggtatgt tggaagctga tgaagcttac ttcaaggaac acggtgaacc attattctcc      420

tcccacatgt tggatttgtc tgaagaaacc gatgaagaaa acatctctac ttgtgtcaag      480

tacttcaaga gaatggccgc tatggaccaa tggttagaaa tggaaatcgg tattaccggt      540

ggtgaagaag atggtgttaa caacgaaaac gctgacaagg aagacttgta caccaagcca      600

gaacaagttt acaacgtcta caaggctttg cacccaatct ctccaaactt ctccattgct      660

gctgctttcg gtaactgtca cggtttgtac gctggtgaca tcgctttgag accagaaatc      720

ttggctgaac accaaaagta caccagagaa caagttggtt gcaaggaaga aaagccattg      780

ttcttggtct tccacggtgg ttccggttct actgtccaag aattccacac tggtattgac      840

aacggtgttg tcaaggtcaa cttggacact gactgtcaat acgcttactt gactggtatc      900

agagactacg tcttgaacaa gaaggactac ataatgtccc cagtcggtaa cccagaaggt      960

ccagaaaagc caaacaagaa gttcttcgac ccaagagtct gggttagaga aggtgaaaag     1020

accatgggtg ctaagatcac caagtctttg gaaactttcc gtaccactaa cactttacca     1080

gaagctttga ttaagtctat gacttctaag ttgtaa                               1116


<210>  11
<211>  360
<212>  PRT
<213>  Pichia pastoris

<400>  11

Met Pro Thr Leu Val Asn Gly Pro Arg Arg Asp Ser Thr Glu Gly Phe 
1               5                   10                  15      


Asp Thr Asp Ile Ile Thr Leu Pro Arg Phe Ile Ile Glu His Gln Lys 
            20                  25                  30          


Gln Phe Lys Asn Ala Thr Gly Asp Phe Thr Leu Val Leu Asn Ala Leu 
        35                  40                  45              


Gln Phe Ala Phe Lys Phe Val Ser His Thr Ile Arg Arg Ala Glu Leu 
    50                  55                  60                  


Val Asn Leu Val Gly Leu Ala Gly Ala Ser Asn Phe Thr Gly Asp Gln 
65                  70                  75                  80  


Gln Lys Lys Leu Asp Val Leu Gly Asp Glu Ile Phe Ile Asn Ala Met 
                85                  90                  95      


Arg Ala Ser Gly Ile Ile Lys Val Leu Val Ser Glu Glu Gln Glu Asp 
            100                 105                 110         


Leu Ile Val Phe Pro Thr Asn Thr Gly Ser Tyr Ala Val Cys Cys Asp 
        115                 120                 125             


Pro Ile Asp Gly Ser Ser Asn Leu Asp Ala Gly Val Ser Val Gly Thr 
    130                 135                 140                 


Ile Ala Ser Ile Phe Arg Leu Leu Pro Asp Ser Ser Gly Thr Ile Asn 
145                 150                 155                 160 


Asp Val Leu Arg Cys Gly Lys Glu Met Val Ala Ala Cys Tyr Ala Met 
                165                 170                 175     


Tyr Gly Ser Ser Thr His Leu Val Leu Thr Leu Gly Asp Gly Val Asp 
            180                 185                 190         


Gly Phe Thr Leu Asp Thr Asn Leu Gly Glu Phe Ile Leu Thr His Pro 
        195                 200                 205             


Asn Leu Arg Ile Pro Pro Gln Lys Ala Ile Tyr Ser Ile Asn Glu Gly 
    210                 215                 220                 


Asn Thr Leu Tyr Trp Asn Glu Thr Ile Arg Thr Phe Ile Glu Lys Val 
225                 230                 235                 240 


Lys Gln Pro Gln Ala Asp Asn Asn Asn Lys Pro Phe Ser Ala Arg Tyr 
                245                 250                 255     


Val Gly Ser Met Val Ala Asp Val His Arg Thr Phe Leu Tyr Gly Gly 
            260                 265                 270         


Leu Phe Ala Tyr Pro Cys Asp Lys Lys Ser Pro Asn Gly Lys Leu Arg 
        275                 280                 285             


Leu Leu Tyr Glu Ala Phe Pro Met Ala Phe Leu Met Glu Gln Ala Gly 
    290                 295                 300                 


Gly Lys Ala Val Asn Asp Arg Gly Glu Arg Ile Leu Asp Leu Val Pro 
305                 310                 315                 320 


Ser His Ile His Asp Lys Ser Ser Ile Trp Leu Gly Ser Ser Gly Glu 
                325                 330                 335     


Ile Asp Lys Phe Leu Asp His Ile Gly Lys Ser Gln Pro Glu Ala Leu 
            340                 345                 350         


Ile Lys Ser Met Thr Ser Lys Leu 
        355                 360 


<210>  12
<211>  1083
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised FBPase DNA sequence

<400>  12
atgccaactc tagtaaatgg accaagaaga gactctaccg aagggtttga taccgatatc       60

atcactcttc ctagattcat aatcgagcac cagaagcaat ttaagaacgc tactggtgat      120

ttcacattag tactgaatgc cttgcaattc gcgttcaaat ttgtatctca caccatcaga      180

cgtgctgaat tggttaactt ggttgggtta gcaggcgctt ccaacttcac tggtgaccag      240

caaaagaagt tggacgttct aggtgatgaa atatttatca atgccatgag ggctagtggg      300

atcatcaagg tccttgtatc tgaagaacag gaagacttga tcgtttttcc cacaaacacg      360

ggctcatacg cagtgtgttg tgatcctatt gatggctcct caaatttgga cgccggtgtc      420

tccgttggaa ctatcgcgtc tatattcaga ctgctaccag actcatcagg tactataaac      480

gacgtactga gatgtggtaa agaaatggta gccgcttgct atgccatgta cggatcctct      540

acgcatctag tattgacatt gggtgatgga gttgatgggt ttaccttaga cacaaacttg      600

ggcgaattca tcttgactca tcctaactta agaattccgc ctcaaaaggc catctactca      660

attaatgaag gtaacaccct ctactggaac gagactataa gaacatttat tgagaaagtc      720

aaacaacccc aagcagacaa caacaacaag cctttctcgg ctaggtatgt tggatccatg      780

gttgctgatg ttcacaggac gtttctttac ggtggccttt tcgcataccc ttgcgacaag      840

aagagcccca acggaaaact gaggttgctt tatgaggcct tcccaatggc tttcttaatg      900

gaacaagcag ggggaaaagc ggtcaacgat cgcggagaga gaatcttgga tttggtgcca      960

agtcatatcc atgacaaatc ttctatttgg ttgggttctt caggtgaaat tgacaaattt     1020

ttagaccata ttggcaagtc acagccagaa gctttgatta agtctatgac ttctaagttg     1080

taa                                                                   1083


<210>  13
<211>  283
<212>  PRT
<213>  Pichia pastoris

<400>  13

Met Pro Ser Leu Thr Pro Arg Cys Ile Ile Val Arg His Gly Gln Thr 
1               5                   10                  15      


Glu Trp Ser Lys Ser Gly Gln Tyr Thr Gly Leu Thr Asp Leu Pro Leu 
            20                  25                  30          


Thr Pro Tyr Gly Glu Gly Gln Met Leu Arg Thr Gly Glu Ser Val Phe 
        35                  40                  45              


Arg Asn Asn Gln Phe Leu Asn Pro Asp Asn Ile Thr Tyr Ile Phe Thr 
    50                  55                  60                  


Ser Pro Arg Leu Arg Ala Arg Gln Thr Val Asp Leu Val Leu Lys Pro 
65                  70                  75                  80  


Leu Ser Asp Glu Gln Arg Ala Lys Ile Arg Val Val Val Asp Asp Asp 
                85                  90                  95      


Leu Arg Glu Trp Glu Tyr Gly Asp Tyr Glu Gly Met Leu Thr Arg Glu 
            100                 105                 110         


Ile Ile Glu Leu Arg Lys Ser Arg Gly Leu Asp Lys Glu Arg Pro Trp 
        115                 120                 125             


Asn Ile Trp Arg Asp Gly Cys Glu Asn Gly Glu Thr Thr Gln Gln Ile 
    130                 135                 140                 


Gly Leu Arg Leu Ser Arg Ala Ile Ala Arg Ile Gln Asn Leu His Arg 
145                 150                 155                 160 


Lys His Gln Ser Glu Gly Arg Ala Ser Asp Ile Met Val Phe Ala His 
                165                 170                 175     


Gly His Ala Leu Arg Tyr Phe Ala Ala Ile Trp Phe Gly Leu Gly Val 
            180                 185                 190         


Gln Lys Lys Cys Glu Thr Ile Glu Glu Ile Gln Asn Val Lys Ser Tyr 
        195                 200                 205             


Asp Asp Asp Thr Val Pro Tyr Val Lys Leu Glu Ser Tyr Arg His Leu 
    210                 215                 220                 


Val Asp Asn Pro Cys Phe Leu Leu Asp Ala Gly Gly Ile Gly Val Leu 
225                 230                 235                 240 


Ser Tyr Ala His His Asn Ile Asp Glu Pro Ala Leu Glu Leu Ala Gly 
                245                 250                 255     


Pro Phe Val Ser Pro Pro Glu Glu Glu Ser Gln His Gly Asp Val Pro 
            260                 265                 270         


Glu Ala Leu Ile Lys Ser Met Thr Ser Lys Leu 
        275                 280             


<210>  14
<211>  852
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised SBPase DNA sequence

<400>  14
atgccttcgc taacccccag atgtatcatt gtcagacacg gtcaaactga atggtccaag       60

tcaggccagt atactggttt gacagatcta ccgttaacgc cctacggtga gggccaaatg      120

ttgaggaccg gtgagagtgt tttccgcaat aatcagtttt tgaatccaga caacatcact      180

tatatcttca cctctccacg tttgcgtgcc aggcaaactg tggatttggt tttgaaacca      240

ttaagcgacg agcaaagagc taagatccgt gtggtggtag acgacgactt gcgagagtgg      300

gagtacggtg actacgaggg aatgctgact cgagaaatca ttgaattgag aaagtcacgc      360

ggtttggaca aggagaggcc atggaatatc tggagagatg ggtgtgagaa cggtgagact      420

actcagcaaa ttgggttgag actttcccgc gctattgcca gaatccagaa cttgcaccgc      480

aagcaccaga gtgagggcag agcatcagac atcatggtct ttgcgcacgg acatgcattg      540

cgttattttg ctgctatttg gtttggactg ggtgtgcaaa agaagtgtga gacgattgaa      600

gaaattcaaa atgtcaaatc ttatgatgac gacacagttc catatgtgaa attggaatct      660

tacagacatt tggtagacaa tccatgtttc ttactggacg ccggtgggat tggtgttttg      720

tcatacgctc accacaacat tgacgaacct gcattggaat tagcaggtcc atttgtctca      780

ccaccagagg aggaatccca gcatggcgat gtgccagaag ctttgattaa gtctatgact      840

tctaagttgt aa                                                          852


<210>  15
<211>  336
<212>  PRT
<213>  Pichia pastoris

<400>  15

Met Ser Asn Ser Leu Glu Gln Leu Lys Ala Ala Gly Thr Val Val Val 
1               5                   10                  15      


Thr Asp Thr Gly Glu Phe Glu Ser Ile Ala Lys Tyr Thr Pro Gln Asp 
            20                  25                  30          


Ala Thr Thr Asn Pro Ser Leu Ile Leu Ala Ala Ser Asn Lys Ala Glu 
        35                  40                  45              


Tyr Ala Lys Leu Ile Asp Ile Ala Val Asp Tyr Ala Lys Lys Gln Gly 
    50                  55                  60                  


Gly Ser Val Glu Glu Gln Ala Asn Ile Ala Leu Asp Arg Leu Leu Ile 
65                  70                  75                  80  


Glu Phe Gly Lys Glu Ile Leu Lys Ile Val Pro Gly Arg Val Ser Thr 
                85                  90                  95      


Glu Val Asp Ala Arg Leu Ser Phe Asp Lys Glu Ala Thr Ile Lys Lys 
            100                 105                 110         


Ala Leu Glu Ile Ile Glu Leu Tyr Lys Ser Val Gly Val Glu Lys Asp 
        115                 120                 125             


Arg Ile Leu Ile Lys Ile Ala Ser Thr Trp Glu Gly Ile Gln Ala Ala 
    130                 135                 140                 


Arg Glu Leu Glu Ala Lys Tyr Gly Ile His Cys Asn Leu Thr Leu Leu 
145                 150                 155                 160 


Phe Ser Phe Val Gln Ala Val Ala Cys Ala Glu Ala Lys Val Thr Leu 
                165                 170                 175     


Ile Ser Pro Phe Val Gly Arg Ile Leu Asp Trp Tyr Lys Ala Ser Thr 
            180                 185                 190         


Gly Lys Asp Tyr Lys Gly Asp Glu Asp Pro Gly Val Gln Ser Val Lys 
        195                 200                 205             


Ala Ile Phe Asn Tyr Tyr Lys Lys Phe Gly Tyr Asp Thr Ile Val Met 
    210                 215                 220                 


Gly Ala Ser Phe Arg Asn Thr Gly Glu Ile Lys Ala Leu Ala Gly Cys 
225                 230                 235                 240 


Asp Phe Leu Thr Ile Ala Pro Lys Leu Leu Glu Glu Leu Leu Asn Ser 
                245                 250                 255     


Lys Glu Pro Val Pro Gln Lys Leu Asp Ala Ser Ala Ala Ser Ser Leu 
            260                 265                 270         


Asp Ile Glu Arg Val Ser Tyr Ile Asp Asp Glu Ala Ala Phe Arg Phe 
        275                 280                 285             


Gly Leu Asn Glu Asp Ala Met Ser Thr Glu Lys Leu Ser Glu Gly Ile 
    290                 295                 300                 


Arg Lys Phe Ser Ala Asp Cys Val Thr Leu Leu Asn Leu Leu Lys Glu 
305                 310                 315                 320 


Lys Val Gln Ala Pro Glu Ala Leu Ile Lys Ser Met Thr Ser Lys Leu 
                325                 330                 335     


<210>  16
<211>  1011
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised TKL DNA sequence

<400>  16
atgtctaact ctttggaaca attgaaggct gctggtactg ttgttgttac tgacactggt       60

gaattcgaat ctattgctaa gtacactcca caagacgcta ctactaaccc atctttgatt      120

ttggctgctt ctaacaaggc tgaatacgct aagttgattg acattgctgt tgactacgct      180

aagaagcaag gtggttctgt tgaagaacaa gctaacattg ctttggacag attgttgatt      240

gaattcggta aggaaatttt gaagattgtt ccaggtagag tttctactga agttgacgct      300

agattgtctt tcgacaagga agctactatt aagaaggctt tggaaattat tgaattgtac      360

aagtctgttg gtgttgaaaa ggacagaatt ttgattaaga ttgcttctac ttgggaaggt      420

attcaagctg ctagagaatt ggaagctaag tacggtattc actgtaactt gactttgttg      480

ttctctttcg ttcaagctgt tgcttgtgct gaagctaagg ttactttgat ttctccattc      540

gttggtagaa ttttggactg gtacaaggct tctactggta aggactacaa gggtgacgaa      600

gacccaggtg ttcaatctgt taaggctatt ttcaactact acaagaagtt cggttacgac      660

actattgtta tgggtgcttc tttcagaaac actggtgaaa ttaaggcttt ggctggttgt      720

gacttcttga ctattgctcc aaagttgttg gaagaattgt tgaactctaa ggaaccagtt      780

ccacaaaagt tggacgcttc tgctgcttct tctttggaca ttgaaagagt ttcttacatt      840

gacgacgaag ctgctttcag attcggtttg aacgaagacg ctatgtctac tgaaaagttg      900

tctgaaggta ttagaaagtt ctctgctgac tgtgttactt tgttgaactt gttgaaggaa      960

aaggttcaag ctccagaagc tttgattaag tctatgactt ctaagttgta a              1011


<210>  17
<211>  270
<212>  PRT
<213>  Pichia pastoris

<400>  17

Met Ala Ala Gly Val Pro Lys Ile Asp Ala Leu Glu Ser Leu Gly Asn 
1               5                   10                  15      


Pro Leu Glu Asp Ala Lys Arg Ala Ala Ala Tyr Arg Ala Val Asp Glu 
            20                  25                  30          


Asn Leu Lys Phe Asp Asp His Lys Ile Ile Gly Ile Gly Ser Gly Ser 
        35                  40                  45              


Thr Val Val Tyr Val Ala Glu Arg Ile Gly Gln Tyr Leu His Asp Pro 
    50                  55                  60                  


Lys Phe Tyr Glu Val Ala Ser Lys Phe Ile Cys Ile Pro Thr Gly Phe 
65                  70                  75                  80  


Gln Ser Arg Asn Leu Ile Leu Asp Asn Lys Leu Gln Leu Gly Ser Ile 
                85                  90                  95      


Glu Gln Tyr Pro Arg Ile Asp Ile Ala Phe Asp Gly Ala Asp Glu Val 
            100                 105                 110         


Asp Glu Asn Leu Gln Leu Ile Lys Gly Gly Gly Ala Cys Leu Phe Gln 
        115                 120                 125             


Glu Lys Leu Val Ser Thr Ser Ala Lys Thr Phe Ile Val Val Ala Asp 
    130                 135                 140                 


Ser Arg Lys Lys Ser Pro Lys His Leu Gly Lys Asn Trp Arg Gln Gly 
145                 150                 155                 160 


Val Pro Ile Glu Ile Val Pro Ser Ser Tyr Val Arg Val Lys Asn Asp 
                165                 170                 175     


Leu Leu Glu Gln Leu His Ala Glu Lys Val Asp Ile Arg Gln Gly Gly 
            180                 185                 190         


Ser Ala Lys Ala Gly Pro Val Val Thr Asp Asn Asn Asn Phe Ile Ile 
        195                 200                 205             


Asp Ala Asp Phe Gly Glu Ile Ser Asp Pro Arg Lys Leu His Arg Glu 
    210                 215                 220                 


Ile Lys Leu Leu Val Gly Val Val Glu Thr Gly Leu Phe Ile Asp Asn 
225                 230                 235                 240 


Ala Ser Lys Ala Tyr Phe Gly Asn Ser Asp Gly Ser Val Glu Val Thr 
                245                 250                 255     


Glu Lys Pro Glu Ala Leu Ile Lys Ser Met Thr Ser Lys Leu 
            260                 265                 270 


<210>  18
<211>  813
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised RKL1 DNA sequence

<400>  18
atggctgccg gtgtcccaaa aattgatgcg ttagaatctt tgggcaatcc tttggaggat       60

gccaagagag ctgcagcata cagagcagtt gatgaaaatt taaaatttga tgatcacaaa      120

attattggaa ttggtagtgg tagcacagtg gtttatgttg ccgaaagaat tggacaatat      180

ttgcatgacc ctaaatttta tgaagtagcg tctaaattca tttgcattcc aacaggattc      240

caatcaagaa acttgatttt ggataacaag ttgcaattag gctccattga acagtatcct      300

cgcattgata tagcgtttga cggtgctgat gaagtggatg agaatttaca attaattaaa      360

ggtggtggtg cttgtctatt tcaagaaaaa ttggttagta ctagtgctaa aaccttcatt      420

gtcgttgctg attcaagaaa aaagtcacca aaacatttag gtaagaactg gaggcaaggt      480

gttcccattg aaattgtacc ttcctcatac gtgagggtca agaatgatct attagaacaa      540

ttgcatgctg aaaaagttga catcagacaa ggaggttctg ctaaagcagg tcctgttgta      600

actgacaata ataacttcat tatcgatgcg gatttcggtg aaatttccga tccaagaaaa      660

ttgcatagag aaatcaaact gttagtgggc gtggtggaaa caggtttatt catcgacaac      720

gcttcaaaag cctacttcgg taattctgac ggtagtgttg aagttaccga aaagccagaa      780

gctttgatta agtctatgac ttctaagttg taa                                   813


<210>  19
<211>  250
<212>  PRT
<213>  Pichia pastoris

<400>  19

Met Val Lys Pro Ile Ile Ala Pro Ser Ile Leu Ala Ser Asp Phe Ala 
1               5                   10                  15      


Asn Leu Gly Cys Glu Cys His Lys Val Ile Asn Ala Gly Ala Asp Trp 
            20                  25                  30          


Leu His Ile Asp Val Met Asp Gly His Phe Val Pro Asn Ile Thr Leu 
        35                  40                  45              


Gly Gln Pro Ile Val Thr Ser Leu Arg Arg Ser Val Pro Arg Pro Gly 
    50                  55                  60                  


Asp Ala Ser Asn Thr Glu Lys Lys Pro Thr Ala Phe Phe Asp Cys His 
65                  70                  75                  80  


Met Met Val Glu Asn Pro Glu Lys Trp Val Asp Asp Phe Ala Lys Cys 
                85                  90                  95      


Gly Ala Asp Gln Phe Thr Phe His Tyr Glu Ala Thr Gln Asp Pro Leu 
            100                 105                 110         


His Leu Val Lys Leu Ile Lys Ser Lys Gly Ile Lys Ala Ala Cys Ala 
        115                 120                 125             


Ile Lys Pro Gly Thr Ser Val Asp Val Leu Phe Glu Leu Ala Pro His 
    130                 135                 140                 


Leu Asp Met Ala Leu Val Met Thr Val Glu Pro Gly Phe Gly Gly Gln 
145                 150                 155                 160 


Lys Phe Met Glu Asp Met Met Pro Lys Val Glu Thr Leu Arg Ala Lys 
                165                 170                 175     


Phe Pro His Leu Asn Ile Gln Val Asp Gly Gly Leu Gly Lys Glu Thr 
            180                 185                 190         


Ile Pro Lys Ala Ala Lys Ala Gly Ala Asn Val Ile Val Ala Gly Thr 
        195                 200                 205             


Ser Val Phe Thr Ala Ala Asp Pro His Asp Val Ile Ser Phe Met Lys 
    210                 215                 220                 


Glu Glu Val Ser Lys Glu Leu Arg Ser Arg Asp Leu Leu Asp Pro Glu 
225                 230                 235                 240 


Ala Leu Ile Lys Ser Met Thr Ser Lys Leu 
                245                 250 


<210>  20
<211>  753
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised RPE1 DNA sequence

<400>  20
atggtcaaac caattatagc tcccagtatc cttgcttctg acttcgccaa cttgggttgc       60

gaatgtcata aggtcatcaa cgccggcgca gattggttac atatcgatgt catggacggc      120

cattttgttc caaacattac tctgggccaa ccaattgtta cctccctacg tcgttctgtg      180

ccacgccctg gcgatgctag caacacagaa aagaagccca ctgcgttctt cgattgtcac      240

atgatggttg aaaatcctga aaaatgggtc gacgattttg ctaaatgtgg tgctgaccaa      300

tttacgttcc actacgaggc cacacaagac cctttgcatt tagttaagtt gattaagtct      360

aagggcatca aagctgcatg cgccatcaaa cctggtactt ctgttgacgt tttatttgaa      420

ctagctcctc atttggatat ggctcttgtt atgactgtgg aacctgggtt tggaggccaa      480

aaattcatgg aagacatgat gccaaaagtg gaaactttga gagccaagtt cccccatttg      540

aatatccaag tcgatggtgg tttgggcaag gagaccatcc cgaaagccgc caaagccggt      600

gccaacgtta ttgtcgctgg taccagtgtt ttcactgcag ctgacccgca cgatgttatc      660

tccttcatga aagaagaagt ctcgaaggaa ttgcgttcta gagatttgct agatccagaa      720

gctttgatta agtctatgac ttctaagttg taa                                   753


<210>  21
<211>  522
<212>  PRT
<213>  Pichia pastoris

<400>  21

Met Ser Gln Pro Pro Lys Trp Thr Thr Ser Asn Gly Ala Pro Val Ser 
1               5                   10                  15      


Asp Val Phe Ala Thr Glu Arg Ala Thr Phe Asp Asn Ala Asn His Ala 
            20                  25                  30          


Asn Asn Ala Pro Lys Val Gly Pro Leu Leu Leu Gln Asp Phe Gln Leu 
        35                  40                  45              


Ile Asp Ser Leu Ala His Phe Asp Arg Glu Arg Ile Pro Glu Arg Val 
    50                  55                  60                  


Val His Ala Lys Gly Ala Gly Ala Phe Gly Glu Phe Glu Val Thr Asp 
65                  70                  75                  80  


Asp Ile Ser Asp Val Cys Ala Ala Lys Phe Leu Asp Thr Ile Gly Lys 
                85                  90                  95      


Lys Thr Arg Ile Phe Thr Arg Phe Ser Thr Val Gly Gly Glu Lys Gly 
            100                 105                 110         


Ser Ala Asp Ser Ala Arg Asp Pro Arg Gly Phe Ser Thr Lys Phe Tyr 
        115                 120                 125             


Thr Glu Glu Gly Asn Leu Asp Leu Val Tyr Asn Asn Thr Pro Ile Phe 
    130                 135                 140                 


Phe Ile Arg Asp Pro Ser Lys Phe Pro His Phe Ile His Thr Gln Lys 
145                 150                 155                 160 


Arg Asn Pro Ala Thr Asn Leu Lys Asp Ala Asn Met Phe Trp Asp Tyr 
                165                 170                 175     


Leu Val Asn Asn Gln Glu Ser Ile His Gln Val Met Tyr Leu Phe Ser 
            180                 185                 190         


Asp Arg Gly Thr Pro Ala Ser Leu Arg Lys Met Asn Gly Tyr Ser Gly 
        195                 200                 205             


His Thr Tyr Lys Trp Tyr Asn Lys Lys Gly Glu Trp Val Tyr Val Gln 
    210                 215                 220                 


Val His Phe Lys Ser Asp Leu Gly Val Val Asn Phe Asn Asn Glu Glu 
225                 230                 235                 240 


Ala Gly Lys Leu Ala Gly Glu Asp Pro Asp Tyr His Thr Gly Asp Leu 
                245                 250                 255     


Phe Asn Ala Ile Glu Arg Gly Glu Tyr Pro Ser Trp Thr Cys Tyr Ile 
            260                 265                 270         


Gln Thr Met Thr Gln Glu Gln Ala Ala Lys Gln Pro Phe Ser Val Phe 
        275                 280                 285             


Asp Leu Thr Lys Val Trp Pro His Lys Asp Phe Pro Leu Arg Arg Phe 
    290                 295                 300                 


Gly Lys Phe Thr Leu Asn Glu Asn Pro Lys Asn Tyr Phe Ala Glu Val 
305                 310                 315                 320 


Glu Gln Ala Ala Phe Ser Pro Ser His Thr Ile Pro Ser Met Gln Pro 
                325                 330                 335     


Ser Ala Asp Pro Val Leu Gln Ser Arg Leu Phe Ser Tyr Pro Asp Thr 
            340                 345                 350         


His Arg His Arg Leu Gly Val Asn Tyr Gln Gln Ile Pro Val Asn Cys 
        355                 360                 365             


Pro Val Ala Pro Val Phe Thr Pro Gln Met Arg Asp Gly Ser Met Thr 
    370                 375                 380                 


Val Asn Gly Asn Leu Gly Ser Thr Pro Asn Tyr Lys Ser Ser Phe Cys 
385                 390                 395                 400 


Pro Phe Ser Thr Glu Ala Gln Ile Gln Thr Asn Ser His Thr Pro Glu 
                405                 410                 415     


Glu Val Leu Ala Ala His Thr Glu Lys Phe His Trp Gly Gly Ile Leu 
            420                 425                 430         


Asp Ser Lys Ser Tyr Asp Phe Glu Gln Pro Arg Ala Leu Trp Lys Val 
        435                 440                 445             


Phe Gly Lys Thr Pro Gly Gln Gln Arg Asn Phe Cys His Asn Val Ala 
    450                 455                 460                 


Val His Val Ala Ala Ala Asn His Glu Ile Gln Asp Arg Val Phe Glu 
465                 470                 475                 480 


Tyr Phe Ser Lys Val Tyr Pro Glu Ile Gly Asp Gln Ile Arg Lys Glu 
                485                 490                 495     


Val Leu Gln Leu Ser Pro Arg Gly Asp Ser Ala Ala Arg Leu Pro Glu 
            500                 505                 510         


Ala Leu Ile Lys Ser Met Thr Ser Lys Leu 
        515                 520         


<210>  22
<211>  1569
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised catalase DNA sequence

<400>  22
atgtctcaac caccaaagtg gactacttct aacggtgctc cagtttctga cgttttcgct       60

actgaaagag ctactttcga caacgctaac cacgctaaca acgctccaaa ggttggtcca      120

ttgttgttgc aagacttcca attgattgac tctttggctc acttcgacag agaaagaatt      180

ccagaaagag ttgttcacgc taagggtgct ggtgctttcg gtgaattcga agttactgac      240

gacatttctg acgtttgtgc tgctaagttc ttggacacta ttggtaagaa gactagaatt      300

ttcactagat tctctactgt tggtggtgaa aagggttctg ctgactctgc tagagaccca      360

agaggtttct ctactaagtt ctacactgaa gaaggtaact tggacttggt ttacaacaac      420

actccaattt tcttcattag agacccatct aagttcccac acttcattca cactcaaaag      480

agaaacccag ctactaactt gaaggacgct aacatgttct gggactactt ggttaacaac      540

caagaatcta ttcaccaagt tatgtacttg ttctctgaca gaggtactcc agcttctttg      600

agaaagatga acggttactc tggtcacact tacaagtggt acaacaagaa gggtgaatgg      660

gtttacgttc aagttcactt caagtctgac ttgggtgttg ttaacttcaa caacgaagaa      720

gctggtaagt tggctggtga agacccagac taccacactg gtgacttgtt caacgctatt      780

gaaagaggtg aatacccatc ttggacttgt tacattcaaa ctatgactca agaacaagct      840

gctaagcaac cattctctgt tttcgacttg actaaggttt ggccacacaa ggacttccca      900

ttgagaagat tcggtaagtt cactttgaac gaaaacccaa agaactactt cgctgaagtt      960

gaacaagctg ctttctctcc atctcacact attccatcta tgcaaccatc tgctgaccca     1020

gttttgcaat ctagattgtt ctcttaccca gacactcaca gacacagatt gggtgttaac     1080

taccaacaaa ttccagttaa ctgtccagtt gctccagttt tcactccaca aatgagagac     1140

ggttctatga ctgttaacgg taacttgggt tctactccaa actacaagtc ttctttctgt     1200

ccattctcta ctgaagctca aattcaaact aactctcaca ctccagaaga agttttggct     1260

gctcacactg aaaagttcca ctggggtggt attttggact ctaagtctta cgacttcgaa     1320

caaccaagag ctttgtggaa ggttttcggt aagactccag gtcaacaaag aaacttctgt     1380

cacaacgttg ctgttcacgt tgctgctgct aaccacgaaa ttcaagacag agttttcgaa     1440

tacttctcta aggtttaccc agaaattggt gaccaaatta gaaaggaagt tttgcaattg     1500

tctccaagag gtgactctgc tgctagattg ccagaagctt tgattaagtc tatgacttct     1560

aagttgtaa                                                             1569


<210>  23
<211>  182
<212>  PRT
<213>  Pichia pastoris

<400>  23

Met Ser Arg Asn Phe Gln Thr Val Lys Arg Gly Asp Arg Phe Pro Thr 
1               5                   10                  15      


Asp Ala Thr Met Phe His Ile Pro Ser Ser Gly Gly Gly Pro Ala Pro 
            20                  25                  30          


Phe Asn Leu Arg Glu Thr Val Gln Gly Lys Arg Phe Ile Val Val Ala 
        35                  40                  45              


Ala Pro Gly Ala Phe Thr Ser Thr Cys His Glu Glu His Leu Pro Pro 
    50                  55                  60                  


Tyr Ile Lys Asn Leu Pro Thr Phe Leu Lys Lys Gly Ile Asp Phe Ile 
65                  70                  75                  80  


Leu Val Ile Thr Ala Asn Asp Ala Phe Val Leu Asn Ser Trp Lys Lys 
                85                  90                  95      


Ala Leu Gly Ala Asp Ser Asp Lys Ile Ile Phe Ala Ser Asp Thr Asn 
            100                 105                 110         


Leu Glu Leu Ala Asn Lys Leu Gly Leu Thr Leu Asp Leu Ser Val Ala 
        115                 120                 125             


Gly Leu Gly Gln Arg Thr Gly Arg Phe Ala Leu Ile Val Gly Lys Asp 
    130                 135                 140                 


Gly Val Val Gln Asn Val Phe Ala Glu Lys Gly Pro Glu Val Lys His 
145                 150                 155                 160 


Ser Ser Ala Asp Arg Val Leu Ala Lys Leu Pro Glu Ala Leu Ile Lys 
                165                 170                 175     


Ser Met Thr Ser Lys Leu 
            180         


<210>  24
<211>  549
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised glutathione peroxidase DNA sequence

<400>  24
atgtctagaa acttccaaac tgttaagaga ggtgacagat tcccaactga cgctactatg       60

ttccacattc catcttctgg tggtggtcca gctccattca acttgagaga aactgttcaa      120

ggtaagagat tcattgttgt tgctgctcca ggtgctttca cttctacttg tcacgaagaa      180

cacttgccac catacattaa gaacttgcca actttcttga agaagggtat tgacttcatt      240

ttggttatta ctgctaacga cgctttcgtt ttgaactctt ggaagaaggc tttgggtgct      300

gactctgaca agattatttt cgcttctgac actaacttgg aattggctaa caagttgggt      360

ttgactttgg acttgtctgt tgctggtttg ggtcaaagaa ctggtagatt cgctttgatt      420

gttggtaagg acggtgttgt tcaaaacgtt ttcgctgaaa agggtccaga agttaagcac      480

tcttctgctg acagagtttt ggctaagttg ccagaagctt tgattaagtc tatgacttct      540

aagttgtaa                                                              549


<210>  25
<211>  350
<212>  PRT
<213>  Pichia pastoris

<400>  25

Met Ser Pro Thr Ile Pro Thr Thr Gln Lys Ala Val Ile Phe Glu Thr 
1               5                   10                  15      


Asn Gly Gly Pro Leu Glu Tyr Lys Asp Ile Pro Val Pro Lys Pro Lys 
            20                  25                  30          


Ser Asn Glu Leu Leu Ile Asn Val Lys Tyr Ser Gly Val Cys His Thr 
        35                  40                  45              


Asp Leu His Ala Trp Lys Gly Asp Trp Pro Leu Asp Asn Lys Leu Pro 
    50                  55                  60                  


Leu Val Gly Gly His Glu Gly Ala Gly Val Val Val Ala Tyr Gly Glu 
65                  70                  75                  80  


Asn Val Thr Gly Trp Glu Ile Gly Asp Tyr Ala Gly Ile Lys Trp Leu 
                85                  90                  95      


Asn Gly Ser Cys Leu Asn Cys Glu Tyr Cys Ile Gln Gly Ala Glu Ser 
            100                 105                 110         


Ser Cys Ala Lys Ala Asp Leu Ser Gly Phe Thr His Asp Gly Ser Phe 
        115                 120                 125             


Gln Gln Tyr Ala Thr Ala Asp Ala Thr Gln Ala Ala Arg Ile Pro Lys 
    130                 135                 140                 


Glu Ala Asp Leu Ala Glu Val Ala Pro Ile Leu Cys Ala Gly Ile Thr 
145                 150                 155                 160 


Val Tyr Lys Ala Leu Lys Thr Ala Asp Leu Arg Ile Gly Gln Trp Val 
                165                 170                 175     


Ala Ile Ser Gly Ala Gly Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr 
            180                 185                 190         


Ala Lys Ala Leu Gly Leu Arg Val Leu Gly Ile Asp Gly Gly Ala Asp 
        195                 200                 205             


Lys Gly Glu Phe Val Lys Ser Leu Gly Ala Glu Val Phe Val Asp Phe 
    210                 215                 220                 


Thr Lys Thr Lys Asp Val Val Ala Glu Val Gln Lys Leu Thr Asn Gly 
225                 230                 235                 240 


Gly Pro His Gly Val Ile Asn Val Ser Val Ser Pro His Ala Ile Asn 
                245                 250                 255     


Gln Ser Val Gln Tyr Val Arg Thr Leu Gly Lys Val Val Leu Val Gly 
            260                 265                 270         


Leu Pro Ser Gly Ala Val Val Asn Ser Asp Val Phe Trp His Val Leu 
        275                 280                 285             


Lys Ser Ile Glu Ile Lys Gly Ser Tyr Val Gly Asn Arg Glu Asp Ser 
    290                 295                 300                 


Ala Glu Ala Ile Asp Leu Phe Thr Arg Gly Leu Val Lys Ala Pro Ile 
305                 310                 315                 320 


Lys Ile Ile Gly Leu Ser Glu Leu Ala Lys Val Tyr Glu Gln Met Glu 
                325                 330                 335     


Ala Gly Ala Ile Ile Gly Arg Tyr Val Val Asp Thr Ser Lys 
            340                 345                 350 


<210>  26
<211>  1053
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised methyl-formate synthesising alcohol dehydrogenase
       DNA sequence

<400>  26
atgtctccaa ctattccaac tactcaaaag gctgttattt tcgaaactaa cggtggtcca       60

ttggaataca aggacattcc agttccaaag ccaaagtcta acgaattgtt gattaacgtt      120

aagtactctg gtgtttgtca cactgacttg cacgcttgga agggtgactg gccattggac      180

aacaagttgc cattggttgg tggtcacgaa ggtgctggtg ttgttgttgc ttacggtgaa      240

aacgttactg gttgggaaat tggtgactac gctggtatta agtggttgaa cggttcttgt      300

ttgaactgtg aatactgtat tcaaggtgct gaatcttctt gtgctaaggc tgacttgtct      360

ggtttcactc acgacggttc tttccaacaa tacgctactg ctgacgctac tcaagctgct      420

agaattccaa aggaagctga cttggctgaa gttgctccaa ttttgtgtgc tggtattact      480

gtttacaagg ctttgaagac tgctgacttg agaattggtc aatgggttgc tatttctggt      540

gctggtggtg gtttgggttc tttggctgtt caatacgcta aggctttggg tttgagagtt      600

ttgggtattg acggtggtgc tgacaagggt gaattcgtta agtctttggg tgctgaagtt      660

ttcgttgact tcactaagac taaggacgtt gttgctgaag ttcaaaagtt gactaacggt      720

ggtccacacg gtgttattaa cgtttctgtt tctccacacg ctattaacca atctgttcaa      780

tacgttagaa ctttgggtaa ggttgttttg gttggtttgc catctggtgc tgttgttaac      840

tctgacgttt tctggcacgt tttgaagtct attgaaatta agggttctta cgttggtaac      900

agagaagact ctgctgaagc tattgacttg ttcactagag gtttggttaa ggctccaatt      960

aagattattg gtttgtctga attggctaag gtttacgaac aaatggaagc tggtgctatt     1020

attggtagat acgttgttga cacttctaag taa                                  1053


<210>  27
<211>  336
<212>  PRT
<213>  Pichia pastoris

<400>  27

Met Ser Asn Ser Leu Glu Gln Leu Lys Ala Ala Gly Thr Val Val Val 
1               5                   10                  15      


Thr Asp Thr Gly Glu Phe Glu Ser Ile Ala Lys Tyr Thr Pro Gln Asp 
            20                  25                  30          


Ala Thr Thr Asn Pro Ser Leu Ile Leu Ala Ala Ser Asn Lys Ala Glu 
        35                  40                  45              


Tyr Ala Lys Leu Ile Asp Ile Ala Val Asp Tyr Ala Lys Lys Gln Gly 
    50                  55                  60                  


Gly Ser Val Glu Glu Gln Ala Asn Ile Ala Leu Asp Arg Leu Leu Ile 
65                  70                  75                  80  


Glu Phe Gly Lys Glu Ile Leu Lys Ile Val Pro Gly Arg Val Ser Thr 
                85                  90                  95      


Glu Val Asp Ala Arg Leu Ser Phe Asp Lys Glu Ala Thr Ile Lys Lys 
            100                 105                 110         


Ala Leu Glu Ile Ile Glu Leu Tyr Lys Ser Val Gly Val Glu Lys Asp 
        115                 120                 125             


Arg Ile Leu Ile Lys Ile Ala Ser Thr Trp Glu Gly Ile Gln Ala Ala 
    130                 135                 140                 


Arg Glu Leu Glu Ala Lys Tyr Gly Ile His Cys Asn Leu Thr Leu Leu 
145                 150                 155                 160 


Phe Ser Phe Val Gln Ala Val Ala Cys Ala Glu Ala Lys Val Thr Leu 
                165                 170                 175     


Ile Ser Pro Phe Val Gly Arg Ile Leu Asp Trp Tyr Lys Ala Ser Thr 
            180                 185                 190         


Gly Lys Asp Tyr Lys Gly Asp Glu Asp Pro Gly Val Gln Ser Val Lys 
        195                 200                 205             


Ala Ile Phe Asn Tyr Tyr Lys Lys Phe Gly Tyr Asp Thr Ile Val Met 
    210                 215                 220                 


Gly Ala Ser Phe Arg Asn Thr Gly Glu Ile Lys Ala Leu Ala Gly Cys 
225                 230                 235                 240 


Asp Phe Leu Thr Ile Ala Pro Lys Leu Leu Glu Glu Leu Leu Asn Ser 
                245                 250                 255     


Lys Glu Pro Val Pro Gln Lys Leu Asp Ala Ser Ala Ala Ser Ser Leu 
            260                 265                 270         


Asp Ile Glu Arg Val Ser Tyr Ile Asp Asp Glu Ala Ala Phe Arg Phe 
        275                 280                 285             


Gly Leu Asn Glu Asp Ala Met Ser Thr Glu Lys Leu Ser Glu Gly Ile 
    290                 295                 300                 


Arg Lys Phe Ser Ala Asp Cys Val Thr Leu Leu Asn Leu Leu Lys Glu 
305                 310                 315                 320 


Lys Val Gln Ala Pro Glu Ala Leu Ile Lys Ser Met Thr Ser Lys Leu 
                325                 330                 335     


<210>  28
<211>  1011
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised transaldolase DNA sequence

<400>  28
atgtctaact ctttggaaca attgaaggct gctggtactg ttgttgttac tgacactggt       60

gaattcgaat ctattgctaa gtacactcca caagacgcta ctactaaccc atctttgatt      120

ttggctgctt ctaacaaggc tgaatacgct aagttgattg acattgctgt tgactacgct      180

aagaagcaag gtggttctgt tgaagaacaa gctaacattg ctttggacag attgttgatt      240

gaattcggta aggaaatttt gaagattgtt ccaggtagag tttctactga agttgacgct      300

agattgtctt tcgacaagga agctactatt aagaaggctt tggaaattat tgaattgtac      360

aagtctgttg gtgttgaaaa ggacagaatt ttgattaaga ttgcttctac ttgggaaggt      420

attcaagctg ctagagaatt ggaagctaag tacggtattc actgtaactt gactttgttg      480

ttctctttcg ttcaagctgt tgcttgtgct gaagctaagg ttactttgat ttctccattc      540

gttggtagaa ttttggactg gtacaaggct tctactggta aggactacaa gggtgacgaa      600

gacccaggtg ttcaatctgt taaggctatt ttcaactact acaagaagtt cggttacgac      660

actattgtta tgggtgcttc tttcagaaac actggtgaaa ttaaggcttt ggctggttgt      720

gacttcttga ctattgctcc aaagttgttg gaagaattgt tgaactctaa ggaaccagtt      780

ccacaaaagt tggacgcttc tgctgcttct tctttggaca ttgaaagagt ttcttacatt      840

gacgacgaag ctgctttcag attcggtttg aacgaagacg ctatgtctac tgaaaagttg      900

tctgaaggta ttagaaagtt ctctgctgac tgtgttactt tgttgaactt gttgaaggaa      960

aaggttcaag ctccagaagc tttgattaag tctatgactt ctaagttgta a              1011


<210>  29
<211>  617
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P. pastoris PRM1 protein sequence with S. cerevisiae MATalpha2 
       nuclear localisation signal

<400>  29

Met Val Ser Gly Gly Thr Ile Phe Lys Ala Glu Gly Ile Trp Arg Gln 
1               5                   10                  15      


Met Val Lys His Tyr His Met His Ser Asn Ser Phe Ile Asn Phe Lys 
            20                  25                  30          


Glu Gly Cys Val Gln Thr Thr Leu Ile Asn Arg Tyr Asn Val Val Ile 
        35                  40                  45              


Val Leu Phe Ile Ile Lys Ile Lys Leu Phe Gln Ala Leu Phe Asn Ser 
    50                  55                  60                  


Ser Leu Ala Val Leu Arg Gln Ser Ser Leu Gly Leu Cys Ser Gln Ile 
65                  70                  75                  80  


Asn Arg Phe Gln Thr Glu Tyr Lys Ser Met Val Asp Glu Ser Leu Asp 
                85                  90                  95      


Ser Leu Ser Leu Phe Ile His Glu Met Thr Asn Lys Ser Ile Gln Val 
            100                 105                 110         


Leu Gly Ser Ile Phe Asn Phe Ile Val Asp Leu Phe Ile Gly Thr Tyr 
        115                 120                 125             


Val Cys Leu Leu Asp Leu Leu Ile Thr Ser Thr Thr Arg Val Ser Ala 
    130                 135                 140                 


Thr Val Ala Glu Glu Ile Val Asp Val Val Asn Asn Thr Ile Thr Thr 
145                 150                 155                 160 


Thr Ala Thr Glu Leu Asn Lys Gln Leu Ser Ser Val Ala Ser Val Ile 
                165                 170                 175     


Asn Arg Val Gly Asn Phe Phe Ser Asp Asp Asn Phe Lys Ala Ile Asp 
            180                 185                 190         


Leu Thr Ile Asp Ser Leu Lys Asn Trp Gln Ile Pro Ser Ser Val Asp 
        195                 200                 205             


Glu Lys Ile Arg Ser Leu Asn Asn Val Asp Val Asn Leu Asp Gly Leu 
    210                 215                 220                 


Leu Asp Gly Val Val Ser Asn Val Thr Asp Lys Ile Ile Lys Thr Arg 
225                 230                 235                 240 


Ser Leu Pro Glu Lys Arg Val Val Asn Gly Ser Phe Ala Leu Asn Glu 
                245                 250                 255     


Ala Cys Ser Ser Asp Thr Val Ser Asp Phe Tyr Asp Ser Leu His Thr 
            260                 265                 270         


Ser Ser Asn Arg Met Phe Thr Leu Ile Leu Ile Leu Ser Val Gly Ser 
        275                 280                 285             


Ile Val Val Ile Thr Leu Val Gln Leu Trp Leu Gln Ser Arg Ser Phe 
    290                 295                 300                 


Arg His Ile Gln Glu Ile Asp Glu Lys Arg Pro Asn Met Glu Val Val 
305                 310                 315                 320 


Ser Thr Phe Glu Asn Arg Tyr Ile His Lys Tyr Cys Gly Ser Gly Tyr 
                325                 330                 335     


Ile Lys Trp Leu Met Leu Tyr Ile Cys Ser Ser Pro Ser Ile Asn Ile 
            340                 345                 350         


Leu Val Leu Phe Phe Phe Ser Leu Phe Ser Tyr Ile Ile Gln Val Ala 
        355                 360                 365             


Ile Val Asn Lys Val Gln Asn Leu Ser Asn Asp Trp Leu Ala Asp Gly 
    370                 375                 380                 


Glu Ser Tyr Asn Asn Asp Asn Leu Asn Gln Tyr Val Gln Tyr Gln Ala 
385                 390                 395                 400 


Lys Ser Tyr Glu Thr Tyr Ile Asn Asp Asn Met Thr Leu Ala Ser Ile 
                405                 410                 415     


His Asn Thr Phe Ser Ser Val Asn Ser Thr Met Asn Asp Phe Ile Thr 
            420                 425                 430         


Asp Val Asn Gly Gly Ile Asn Gly Ile Phe Arg Asp Ser Ile Val Gly 
        435                 440                 445             


Asp Met Val Asn Gly Val Val Tyr Cys Val Ile Gly Arg Lys Leu Glu 
    450                 455                 460                 


Ala Val Asn Lys Gly Phe Asp Trp Leu Thr Lys Ser Thr Glu Leu Lys 
465                 470                 475                 480 


Ile Pro Thr Glu Asn Tyr Val Ile Ser Asn Gln Asp Gln Asp Asp Leu 
                485                 490                 495     


Met Ser Lys Met Ala Glu Gly Ile Ser Ala Leu Cys Lys Tyr Tyr Lys 
            500                 505                 510         


Gln Asn Leu Thr Trp Glu Leu Trp Gln Cys Leu Leu Phe Gly Leu Leu 
        515                 520                 525             


Trp Thr Phe Gln Leu Met Ile Gly Ile Leu Ile Ser Lys Phe Gln Arg 
    530                 535                 540                 


Gln Pro Glu Gln Arg Tyr Asp Asp Asn Pro Phe Ile His Pro Glu Phe 
545                 550                 555                 560 


Ile Pro Phe Lys Pro Tyr Glu Thr Tyr His His Thr Lys Asn Asp Val 
                565                 570                 575     


Ile Ala Glu Thr Leu Asn Arg Ile Lys Arg Asn Glu Ser Val Pro Ser 
            580                 585                 590         


Phe Lys Ala Asp Ser Leu Ser Leu Ser Ser Met Leu Asp Asn Ser Ala 
        595                 600                 605             


Arg Arg Pro Lys Lys Ile Pro Ile Lys 
    610                 615         


<210>  30
<211>  1854
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised PRM1 DNA sequence

<400>  30
atggtttctg gtggtactat ttttaaagct gaaggtattt ggagacaaat ggttaaacat       60

tatcatatgc attctaattc ttttattaat tttaaagaag gttgtgttca aactactttg      120

attaatagat ataatgttgt tattgttttg tttattatta aaattaaatt gtttcaagct      180

ttgtttaatt cttctttggc tgttttgaga caatcttctt tgggtttgtg ttctcaaatt      240

aatagatttc aaactgaata taaatctatg gttgatgaat ctttggattc tttgtctttg      300

tttattcatg aaatgactaa taaatctatt caagttttgg gttctatttt taattttatt      360

gttgatttgt ttattggtac ttatgtttgt ttgttggatt tgttgattac ttctactact      420

agagtttctg ctactgttgc tgaagaaatt gttgatgttg ttaataatac tattactact      480

actgctactg aattgaataa acaattgtct tctgttgctt ctgttattaa tagagttggt      540

aatttttttt ctgatgataa ttttaaagct attgatttga ctattgattc tttgaaaaat      600

tggcaaattc catcttctgt tgatgaaaaa attagatctt tgaataatgt tgatgttaat      660

ttggatggtt tgttggatgg tgttgtttct aatgttactg ataaaattat taaaactaga      720

tctttgccag aaaaaagagt tgttaatggt tcttttgctt tgaatgaagc ttgttcttct      780

gatactgttt ctgattttta tgattctttg catacttctt ctaatagaat gtttactttg      840

attttgattt tgtctgttgg ttctattgtt gttattactt tggttcaatt gtggttgcaa      900

tctagatctt ttagacatat tcaagaaatt gatgaaaaaa gaccaaatat ggaagttgtt      960

tctacttttg aaaatagata tattcataaa tattgtggtt ctggttatat taaatggttg     1020

atgttgtata tttgttcttc tccatctatt aatattttgg ttttgttttt tttttctttg     1080

ttttcttata ttattcaagt tgctattgtt aataaagttc aaaatttgtc taatgattgg     1140

ttggctgatg gtgaatctta taataatgat aatttgaatc aatatgttca atatcaagct     1200

aaatcttatg aaacttatat taatgataat atgactttgg cttctattca taatactttt     1260

tcttctgtta attctactat gaatgatttt attactgatg ttaatggtgg tattaatggt     1320

atttttagag attctattgt tggtgatatg gttaatggtg ttgtttattg tgttattggt     1380

agaaaattgg aagctgttaa taaaggtttt gattggttga ctaaatctac tgaattgaaa     1440

attccaactg aaaattatgt tatttctaat caagatcaag atgatttgat gtctaaaatg     1500

gctgaaggta tttctgcttt gtgtaaatat tataaacaaa atttgacttg ggaattgtgg     1560

caatgtttgt tgtttggttt gttgtggact tttcaattga tgattggtat tttgatttct     1620

aaatttcaaa gacaaccaga acaaagatat gatgataatc catttattca tccagaattt     1680

attccattta aaccatatga aacttatcat catactaaaa atgatgttat tgctgaaact     1740

ttgaatagaa ttaaaagaaa tgaatctgtt ccatctttta aagctgattc tttgtctttg     1800

tcttctatgt tggataattc tgctagaaga ccaaaaaaaa ttccaattaa ataa           1854


<210>  31
<211>  641
<212>  PRT
<213>  Pichia pastoris

<400>  31

Gly Cys Ala Ala Ala Thr Cys Ala Thr Gly Gly Ala Cys Thr Gly Gly 
1               5                   10                  15      


Ala Thr Gly Cys Gly Gly Cys Cys Ala Gly Cys Ala Cys Ala Thr Cys 
            20                  25                  30          


Cys Cys Ala Thr Cys Ala Gly Cys Cys Ala Thr Gly Ala Ala Thr Ala 
        35                  40                  45              


Ala Cys Ala Cys Cys Ala Gly Ala Cys Ala Ala Gly Ala Ala Thr Thr 
    50                  55                  60                  


Gly Thr Gly Gly Thr Gly Thr Ala Cys Thr Thr Gly Thr Ala Cys Thr 
65                  70                  75                  80  


Cys Ala Thr Ala Thr Ala Cys Thr Gly Gly Ala Thr Ala Cys Ala Ala 
                85                  90                  95      


Ala Cys Thr Ala Thr Cys Cys Ala Cys Ala Ala Thr Ala Cys Cys Cys 
            100                 105                 110         


Thr Cys Cys Cys Cys Gly Cys Gly Cys Thr Gly Gly Thr Ala Cys Gly 
        115                 120                 125             


Gly Gly Gly Thr Cys Thr Gly Cys Thr Cys Gly Thr Cys Gly Thr Thr 
    130                 135                 140                 


Cys Thr Ala Thr Thr Thr Cys Cys Gly Ala Gly Thr Ala Gly Gly Ala 
145                 150                 155                 160 


Thr Ala Ala Cys Thr Thr Gly Ala Thr Ala Thr Thr Gly Ala Ala Gly 
                165                 170                 175     


Thr Cys Thr Ala Thr Gly Gly Gly Thr Cys Ala Thr Gly Ala Gly Thr 
            180                 185                 190         


Thr Gly Ala Thr Gly Ala Ala Thr Ala Ala Cys Thr Gly Ala Gly Thr 
        195                 200                 205             


Thr Gly Gly Gly Gly Ala Ala Gly Thr Thr Cys Ala Cys Cys Ala Gly 
    210                 215                 220                 


Ala Thr Gly Ala Gly Thr Thr Ala Gly Thr Ala Ala Gly Gly Gly Thr 
225                 230                 235                 240 


Thr Thr Cys Ala Ala Thr Gly Thr Gly Thr Gly Thr Ala Gly Gly Ala 
                245                 250                 255     


Thr Gly Ala Ala Ala Thr Ala Thr Ala Thr Gly Gly Thr Thr Cys Thr 
            260                 265                 270         


Thr Gly Ala Thr Gly Gly Ala Cys Gly Ala Thr Thr Gly Cys Cys Gly 
        275                 280                 285             


Thr Ala Gly Cys Ala Thr Cys Ala Ala Ala Thr Thr Thr Cys Thr Gly 
    290                 295                 300                 


Ala Thr Ala Cys Cys Cys Cys Gly Ala Gly Cys Cys Gly Thr Gly Ala 
305                 310                 315                 320 


Thr Gly Thr Cys Thr Thr Cys Gly Thr Thr Ala Thr Cys Thr Gly Gly 
                325                 330                 335     


Thr Gly Thr Thr Gly Thr Thr Gly Thr Thr Ala Thr Cys Ala Gly Thr 
            340                 345                 350         


Thr Ala Thr Cys Thr Gly Thr Gly Cys Gly Gly Cys Thr Gly Cys Thr 
        355                 360                 365             


Ala Thr Cys Thr Thr Thr Thr Cys Thr Ala Gly Gly Thr Thr Thr Thr 
    370                 375                 380                 


Cys Thr Ala Cys Gly Thr Ala Ala Gly Cys Thr Thr Thr Cys Thr Ala 
385                 390                 395                 400 


Ala Gly Cys Cys Gly Cys Ala Cys Gly Ala Cys Ala Thr Gly Cys Cys 
                405                 410                 415     


Gly Ala Ala Thr Gly Thr Thr Gly Cys Gly Thr Gly Ala Gly Ala Thr 
            420                 425                 430         


Gly Ala Gly Ala Ala Thr Ala Gly Ala Thr Gly Ala Thr Ala Gly Ala 
        435                 440                 445             


Thr Gly Thr Cys Thr Gly Ala Ala Gly Gly Ala Ala Ala Ala Ala Ala 
    450                 455                 460                 


Gly Ala Cys Gly Ala Ala Thr Ala Gly Thr Ala Ala Thr Cys Thr Thr 
465                 470                 475                 480 


Cys Gly Thr Ala Gly Ala Ala Gly Thr Thr Thr Thr Ala Ala Ala Gly 
                485                 490                 495     


Gly Thr Gly Ala Thr Ala Gly Cys Ala Ala Ala Thr Ala Ala Thr Thr 
            500                 505                 510         


Gly Ala Thr Cys Ala Gly Thr Gly Gly Ala Ala Ala Ala Ala Ala Thr 
        515                 520                 525             


Thr Gly Cys Ala Cys Ala Ala Thr Gly Ala Gly Thr Gly Gly Ala Ala 
    530                 535                 540                 


Ala Ala Thr Gly Gly Thr Thr Thr Gly Cys Ala Ala Thr Gly Ala Gly 
545                 550                 555                 560 


Cys Ala Ala Thr Thr Ala Thr Gly Ala Gly Thr Thr Thr Ala Ala Gly 
                565                 570                 575     


Cys Cys Thr Cys Thr Thr Gly Gly Ala Ala Ala Cys Thr Gly Thr Cys 
            580                 585                 590         


Ala Ala Gly Gly Thr Gly Ala Ala Ala Gly Ala Thr Thr Gly Ala Gly 
        595                 600                 605             


Cys Thr Thr Thr Ala Gly Ala Cys Gly Ala Ala Thr Gly Thr Gly Ala 
    610                 615                 620                 


Thr Thr Gly Thr Ala Thr Ala Cys Thr Gly Thr Thr Thr Cys Ala Gly 
625                 630                 635                 640 


Gly 
    


<210>  32
<211>  893
<212>  PRT
<213>  Artificial Sequence

<220>
<223>   P. pastoris MIT1 protein sequence with S. cerevisiae MATalpha2 
       nuclear localisation signal

<400>  32

Met Ser Thr Ala Ala Pro Ile Lys Glu Glu Ser Gln Phe Ala His Leu 
1               5                   10                  15      


Thr Leu Met Asn Lys Asp Leu Pro Ser Asn Ala Lys Gln Ala Lys Ser 
            20                  25                  30          


Lys Val Ser Ala Ala Pro Ala Lys Thr Ser Ser Arg Ser Ala Gly Gly 
        35                  40                  45              


Ser Gly Asn Asn Asn Ala Ala Pro Val Lys Lys Arg Val Arg Thr Gly 
    50                  55                  60                  


Cys Leu Thr Cys Arg Lys Lys His Lys Lys Cys Asp Glu Asn Arg Asn 
65                  70                  75                  80  


Pro Lys Cys Asp Phe Cys Thr Leu Lys Gly Leu Glu Cys Val Trp Pro 
                85                  90                  95      


Glu Asn Asn Lys Lys Asn Ile Phe Val Asn Asn Ser Met Lys Asp Phe 
            100                 105                 110         


Leu Gly Lys Lys Pro Val Glu Gly Ala Asp Ser Leu Asn Leu Ala Met 
        115                 120                 125             


Asn Leu Gln Gln Gln Gln Ser Ser Asn Ala Met Gly Asn Gln Ser Leu 
    130                 135                 140                 


Ser Ser Ile Gly Leu Glu Ser Phe Gly Tyr Gly Ser Gly Ile Lys Asn 
145                 150                 155                 160 


Glu Phe Asn Phe Gln Asp Leu Ile Gly Ser Asn Ser Gly Ser Ser Asp 
                165                 170                 175     


Pro Thr Phe Thr Val Asp Ala Asp Glu Ala Gln Lys Phe Asp Ile Ser 
            180                 185                 190         


Ser Lys Asn Ser Arg Lys Arg Gln Lys Leu Gly Leu Leu Pro Val Gly 
        195                 200                 205             


Asn Ala Ala Ser His Leu Asn Gly Phe Asn Gly Ile Ser Asn Gly Lys 
    210                 215                 220                 


Ser His Ser Phe Ser Ser Pro Ser Gly Thr Asn Asp Asp Glu Leu Ser 
225                 230                 235                 240 


Gly Leu Met Phe Asn Ser Pro Ser Phe Asn Pro Leu Thr Val Asn Asp 
                245                 250                 255     


Ser Thr Asn Asn Ser Asn His Asn Ile Gly Leu Ser Pro Met Ser Cys 
            260                 265                 270         


Leu Phe Ser Thr Val Gln Glu Ala Ser Gln Lys Lys His Ala Asn Ser 
        275                 280                 285             


Ser Arg His Phe Ser Tyr Pro Ser Gly Pro Glu Asp Leu Trp Phe Asn 
    290                 295                 300                 


Glu Phe Gln Lys Gln Ala Leu Thr Ala Asn Gly Glu Asn Ala Val Gln 
305                 310                 315                 320 


Gln Gly Asp Asp Ala Ser Lys Asp Thr Thr Val Ile Pro Lys Asp Glu 
                325                 330                 335     


Ser Ser Asn Ser Ser Ile Phe Ser Ser Arg Ser Ser Ala Ala Ser Ser 
            340                 345                 350         


Asn Ser Gly Asp Asp Val Gly Arg Met Gly Pro Phe Ser Lys Gly Pro 
        355                 360                 365             


Glu Ile Glu Phe Asn Tyr Asp Ser Phe Leu Glu Ser Leu Lys Ala Glu 
    370                 375                 380                 


Ser Pro Ser Ser Ser Lys Tyr Asn Leu Pro Asp Thr Leu Lys Glu Tyr 
385                 390                 395                 400 


Met Thr Leu Ser Ser Ser Tyr Leu Asn Thr Gln His Ser Asp Thr Leu 
                405                 410                 415     


Ala Asn Gly Thr Asn Gly Asn Tyr Ala Ser Thr Val Ser Asn Asn Leu 
            420                 425                 430         


Ser Leu Ser Leu Asn Ser Phe Ser Phe Ser Asp Lys Phe Ser Leu Ser 
        435                 440                 445             


Pro Pro Thr Ile Thr Asp Ala Glu Lys Phe Ser Leu Met Arg Asn Phe 
    450                 455                 460                 


Ile Asp Asn Ile Ser Pro Trp Phe Asp Thr Phe Asp Asn Thr Lys Gln 
465                 470                 475                 480 


Phe Gly Thr Lys Ile Pro Ile Leu Ala Lys Lys Cys Ser Ser Leu Tyr 
                485                 490                 495     


Tyr Ala Ile Leu Ala Ile Ser Ser Arg Gln Arg Glu Arg Ile Arg Lys 
            500                 505                 510         


Glu His Asn Glu Lys Thr Leu Gln Cys Tyr Gln Tyr Ser Leu Gln Gln 
        515                 520                 525             


Leu Ile Pro Thr Val Gln Ser Ser Asn Asn Ile Glu Tyr Ile Ile Thr 
    530                 535                 540                 


Cys Ile Leu Leu Ser Val Phe His Ile Met Ser Ser Glu Pro Ser Thr 
545                 550                 555                 560 


Gln Arg Asp Ile Ile Ala Ser Leu Ala Lys Tyr Ile Gln Ser Cys Asn 
                565                 570                 575     


Ile Asn Gly Phe Thr Ser Asn Asp Lys Leu Glu Lys Ser Ile Phe Trp 
            580                 585                 590         


Asn Tyr Val Asn Leu Asp Leu Ala Thr Cys Thr Ile Gly Glu Glu Ser 
        595                 600                 605             


Met Val Ile Pro Phe Ser Tyr Trp Val Lys Glu Thr Thr Asp Tyr Lys 
    610                 615                 620                 


Thr Ile Gln Asp Val Lys Pro Phe Phe Thr Lys Lys Thr Ser Thr Thr 
625                 630                 635                 640 


Thr Asp Asp Asp Leu Asp Asp Met Tyr Ala Ile Tyr Ile Leu Tyr Ile 
                645                 650                 655     


Ser Gly Arg Ile Ile Asn Leu Leu Asn Cys Arg Asp Ala Lys Leu Asn 
            660                 665                 670         


Phe Glu Pro Lys Trp Glu Phe Leu Trp Asn Glu Leu Asn Glu Trp Glu 
        675                 680                 685             


Leu Asn Lys Pro Leu Thr Phe Gln Ser Ile Val Gln Phe Lys Ala Asn 
    690                 695                 700                 


Asp Glu Ser Gln Gly Gly Ser Thr Phe Pro Thr Val Leu Phe Ser Asn 
705                 710                 715                 720 


Ser Arg Ser Cys Tyr Ser Asn Gln Leu Tyr His Met Ser Tyr Ile Ile 
                725                 730                 735     


Leu Val Gln Asn Lys Pro Arg Leu Tyr Lys Ile Pro Phe Thr Thr Val 
            740                 745                 750         


Ser Ala Ser Met Ser Ser Pro Ser Asp Asn Arg Ala Gly Ala Ser Ala 
        755                 760                 765             


Ser Ser Thr Pro Ala Ser Asp His His Ala Ser Gly Asp His Leu Ser 
    770                 775                 780                 


Pro Arg Ser Ile Glu Pro Ser Val Ser Thr Thr Leu Ser Pro Pro Pro 
785                 790                 795                 800 


Asn Ala Asn Gly Gly Gly Asn Lys Phe Arg Ser Thr Leu Trp His Ala 
                805                 810                 815     


Lys Gln Ile Cys Gly Ile Ser Ile Asn Asn Asn His Asn Ser Asn Leu 
            820                 825                 830         


Ala Ala Lys Val Asn Ser Leu Gln Pro Leu Trp His Ala Gly Lys Leu 
        835                 840                 845             


Ile Ser Ser Lys Ser Glu His Thr Gln Leu Leu Lys Leu Leu Asn Asn 
    850                 855                 860                 


Leu Glu Cys Ala Thr Gly Trp Pro Met Asn Trp Lys Gly Lys Glu Leu 
865                 870                 875                 880 


Ile Asp Tyr Trp Asn Val Glu Glu Lys Ile Pro Ile Lys 
                885                 890             


<210>  33
<211>  2682
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised MIT1 DNA sequence

<400>  33
atgtctactg ctgctccaat taaagaagaa tctcaatttg ctcatttgac tttgatgaat       60

aaagatttgc catctaatgc taaacaagct aaatctaaag tttctgctgc tccagctaaa      120

acttcttcta gatctgctgg tggttctggt aataataatg ctgctccagt taaaaaaaga      180

gttagaactg gttgtttgac ttgtagaaaa aaacataaaa aatgtgatga aaatagaaat      240

ccaaaatgtg atttttgtac tttgaaaggt ttggaatgtg tttggccaga aaataataaa      300

aaaaatattt ttgttaataa ttctatgaaa gattttttgg gtaaaaaacc agttgaaggt      360

gctgattctt tgaatttggc tatgaatttg caacaacaac aatcttctaa tgctatgggt      420

aatcaatctt tgtcttctat tggtttggaa tcttttggtt atggttctgg tattaaaaat      480

gaatttaatt ttcaagattt gattggttct aattctggtt cttctgatcc aacttttact      540

gttgatgctg atgaagctca aaaatttgat atttcttcta aaaattctag aaaaagacaa      600

aaattgggtt tgttgccagt tggtaatgct gcttctcatt tgaatggttt taatggtatt      660

tctaatggta aatctcattc tttttcttct ccatctggta ctaatgatga tgaattgtct      720

ggtttgatgt ttaattctcc atcttttaat ccattgactg ttaatgattc tactaataat      780

tctaatcata atattggttt gtctccaatg tcttgtttgt tttctactgt tcaagaagct      840

tctcaaaaaa aacatgctaa ttcttctaga catttttctt atccatctgg tccagaagat      900

ttgtggttta atgaatttca aaaacaagct ttgactgcta atggtgaaaa tgctgttcaa      960

caaggtgatg atgcttctaa agatactact gttattccaa aagatgaatc ttctaattct     1020

tctatttttt cttctagatc ttctgctgct tcttctaatt ctggtgatga tgttggtaga     1080

atgggtccat tttctaaagg tccagaaatt gaatttaatt atgattcttt tttggaatct     1140

ttgaaagctg aatctccatc ttcttctaaa tataatttgc cagatacttt gaaagaatat     1200

atgactttgt cttcttctta tttgaatact caacattctg atactttggc taatggtact     1260

aatggtaatt atgcttctac tgtttctaat aatttgtctt tgtctttgaa ttctttttct     1320

ttttctgata aattttcttt gtctccacca actattactg atgctgaaaa attttctttg     1380

atgagaaatt ttattgataa tatttctcca tggtttgata cttttgataa tactaaacaa     1440

tttggtacta aaattccaat tttggctaaa aaatgttctt ctttgtatta tgctattttg     1500

gctatttctt ctagacaaag agaaagaatt agaaaagaac ataatgaaaa aactttgcaa     1560

tgttatcaat attctttgca acaattgatt ccaactgttc aatcttctaa taatattgaa     1620

tatattatta cttgtatttt gttgtctgtt tttcatatta tgtcttctga accatctact     1680

caaagagata ttattgcttc tttggctaaa tatattcaat cttgtaatat taatggtttt     1740

acttctaatg ataaattgga aaaatctatt ttttggaatt atgttaattt ggatttggct     1800

acttgtacta ttggtgaaga atctatggtt attccatttt cttattgggt taaagaaact     1860

actgattata aaactattca agatgttaaa ccatttttta ctaaaaaaac ttctactact     1920

actgatgatg atttggatga tatgtatgct atttatattt tgtatatttc tggtagaatt     1980

attaatttgt tgaattgtag agatgctaaa ttgaattttg aaccaaaatg ggaatttttg     2040

tggaatgaat tgaatgaatg ggaattgaat aaaccattga cttttcaatc tattgttcaa     2100

tttaaagcta atgatgaatc tcaaggtggt tctacttttc caactgtttt gttttctaat     2160

tctagatctt gttattctaa tcaattgtat catatgtctt atattatttt ggttcaaaat     2220

aaaccaagat tgtataaaat tccatttact actgtttctg cttctatgtc ttctccatct     2280

gataatagag ctggtgcttc tgcttcttct actccagctt ctgatcatca tgcttctggt     2340

gatcatttgt ctccaagatc tattgaacca tctgtttcta ctactttgtc tccaccacca     2400

aatgctaatg gtggtggtaa taaatttaga tctactttgt ggcatgctaa acaaatttgt     2460

ggtatttcta ttaataataa tcataattct aatttggctg ctaaagttaa ttctttgcaa     2520

ccattgtggc atgctggtaa attgatttct tctaaatctg aacatactca attgttgaaa     2580

ttgttgaata atttggaatg tgctactggt tggccaatga attggaaagg taaagaattg     2640

attgattatt ggaatgttga agaaaagatt ccaattaagt aa                        2682


<210>  34
<211>  1503
<212>  PRT
<213>  Pichia pastoris

<400>  34

Gly Cys Ala Gly Thr Cys Ala Ala Ala Ala Ala Thr Gly Gly Cys Ala 
1               5                   10                  15      


Ala Cys Cys Cys Cys Cys Gly Gly Ala Thr Ala Thr Thr Gly Gly Ala 
            20                  25                  30          


Gly Gly Gly Ala Ala Gly Ala Ala Gly Ala Gly Thr Gly Thr Gly Gly 
        35                  40                  45              


Gly Gly Gly Ala Gly Ala Cys Ala Cys Cys Thr Gly Thr Cys Ala Cys 
    50                  55                  60                  


Thr Gly Cys Ala Gly Cys Thr Ala Thr Gly Ala Gly Gly Ala Gly Thr 
65                  70                  75                  80  


Thr Thr Gly Ala Thr Thr Thr Ala Thr Thr Gly Gly Ala Ala Gly Cys 
                85                  90                  95      


Ala Thr Thr Thr Gly Thr Thr Ala Ala Thr Gly Thr Gly Gly Gly Thr 
            100                 105                 110         


Gly Ala Ala Thr Thr Cys Thr Cys Thr Gly Cys Gly Ala Cys Cys Thr 
        115                 120                 125             


Ala Cys Ala Thr Ala Ala Thr Ala Cys Gly Thr Gly Cys Ala Thr Ala 
    130                 135                 140                 


Ala Thr Ala Thr Gly Thr Gly Cys Ala Thr Gly Ala Ala Ala Ala Gly 
145                 150                 155                 160 


Ala Cys Ala Ala Cys Ala Ala Gly Ala Thr Ala Ala Ala Thr Thr Thr 
                165                 170                 175     


Ala Gly Thr Ala Ala Ala Ala Thr Ala Thr Gly Gly Gly Gly Thr Ala 
            180                 185                 190         


Ala Thr Ala Gly Ala Gly Ala Gly Gly Gly Cys Thr Gly Gly Ala Cys 
        195                 200                 205             


Thr Gly Ala Ala Cys Gly Thr Thr Gly Ala Thr Gly Gly Cys Ala Ala 
    210                 215                 220                 


Gly Ala Cys Thr Gly Ala Gly Gly Gly Ala Gly Ala Ala Ala Ala Cys 
225                 230                 235                 240 


Ala Thr Ala Ala Ala Ala Gly Cys Ala Ala Gly Thr Gly Gly Ala Ala 
                245                 250                 255     


Thr Thr Ala Gly Thr Ala Cys Ala Cys Ala Ala Thr Thr Ala Gly Thr 
            260                 265                 270         


Gly Cys Thr Gly Gly Ala Thr Thr Gly Thr Thr Gly Gly Ala Cys Thr 
        275                 280                 285             


Gly Thr Ala Thr Ala Gly Gly Gly Gly Thr Thr Cys Thr Gly Thr Ala 
    290                 295                 300                 


Thr Thr Cys Gly Thr Ala Gly Gly Thr Cys Cys Cys Thr Gly Ala Thr 
305                 310                 315                 320 


Thr Gly Ala Thr Ala Gly Thr Ala Ala Thr Gly Thr Gly Thr Gly Thr 
                325                 330                 335     


Ala Ala Gly Thr Gly Ala Ala Gly Gly Gly Ala Ala Ala Ala Ala Ala 
            340                 345                 350         


Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala Thr Thr Cys Ala 
        355                 360                 365             


Ala Ala Ala Ala Cys Thr Gly Gly Gly Gly Ala Ala Ala Ala Gly Ala 
    370                 375                 380                 


Ala Cys Thr Cys Cys Thr Thr Ala Ala Ala Ala Ala Thr Thr Thr Thr 
385                 390                 395                 400 


Cys Ala Thr Ala Thr Gly Thr Ala Thr Gly Ala Thr Gly Ala Ala Ala 
                405                 410                 415     


Gly Ala Cys Thr Gly Cys Thr Gly Cys Ala Gly Gly Ala Gly Ala Thr 
            420                 425                 430         


Gly Thr Thr Cys Ala Ala Gly Ala Thr Thr Gly Ala Ala Ala Ala Ala 
        435                 440                 445             


Thr Thr Ala Ala Ala Gly Cys Thr Ala Gly Cys Gly Ala Cys Cys Thr 
    450                 455                 460                 


Ala Cys Gly Ala Gly Thr Cys Cys Ala Thr Ala Thr Ala Gly Thr Gly 
465                 470                 475                 480 


Thr Thr Gly Gly Ala Ala Cys Gly Gly Ala Ala Thr Cys Thr Gly Gly 
                485                 490                 495     


Ala Gly Gly Thr Gly Thr Gly Ala Ala Cys Ala Cys Cys Gly Thr Cys 
            500                 505                 510         


Ala Thr Thr Cys Cys Cys Thr Ala Ala Thr Cys Thr Ala Ala Gly Thr 
        515                 520                 525             


Thr Thr Cys Thr Gly Thr Cys Gly Ala Gly Cys Cys Gly Thr Thr Thr 
    530                 535                 540                 


Gly Ala Ala Ala Ala Gly Thr Thr Cys Cys Thr Cys Thr Thr Cys Gly 
545                 550                 555                 560 


Thr Cys Ala Ala Thr Ala Ala Ala Gly Thr Thr Thr Gly Ala Cys Thr 
                565                 570                 575     


Thr Thr Gly Ala Thr Cys Gly Ala Cys Ala Gly Ala Gly Thr Thr Gly 
            580                 585                 590         


Ala Gly Cys Thr Ala Ala Thr Cys Gly Gly Ala Ala Ala Thr Ala Cys 
        595                 600                 605             


Thr Cys Cys Cys Gly Ala Ala Gly Ala Thr Ala Cys Ala Cys Cys Cys 
    610                 615                 620                 


Gly Ala Gly Cys Ala Gly Thr Gly Ala Ala Gly Cys Gly Gly Gly Gly 
625                 630                 635                 640 


Cys Ala Thr Cys Ala Gly Thr Ala Ala Ala Ala Ala Thr Thr Ala Ala 
                645                 650                 655     


Gly Gly Gly Gly Thr Gly Thr Thr Gly Ala Gly Thr Gly Cys Gly Gly 
            660                 665                 670         


Thr Thr Thr Gly Cys Gly Cys Gly Cys Ala Gly Thr Ala Gly Thr Thr 
        675                 680                 685             


Ala Gly Cys Ala Thr Cys Ala Ala Thr Gly Cys Cys Ala Gly Ala Cys 
    690                 695                 700                 


Gly Thr Ala Gly Ala Ala Ala Thr Cys Ala Thr Ala Cys Ala Thr Thr 
705                 710                 715                 720 


Cys Thr Gly Gly Thr Thr Thr Cys Ala Gly Ala Ala Thr Cys Ala Gly 
                725                 730                 735     


Ala Cys Gly Ala Cys Ala Thr Cys Gly Ala Gly Ala Gly Ala Gly Ala 
            740                 745                 750         


Thr Ala Cys Ala Cys Cys Ala Gly Cys Ala Cys Cys Ala Cys Thr Thr 
        755                 760                 765             


Gly Ala Ala Cys Thr Cys Cys Cys Cys Cys Ala Ala Thr Gly Ala Cys 
    770                 775                 780                 


Gly Gly Thr Gly Cys Gly Gly Thr Gly Gly Thr Gly Gly Gly Gly Gly 
785                 790                 795                 800 


Thr Gly Ala Ala Cys Ala Gly Gly Thr Ala Gly Ala Ala Cys Thr Gly 
                805                 810                 815     


Thr Ala Gly Ala Thr Gly Thr Gly Ala Cys Cys Ala Ala Thr Cys Ala 
            820                 825                 830         


Cys Thr Gly Thr Cys Ala Cys Gly Thr Ala Thr Thr Ala Thr Ala Thr 
        835                 840                 845             


Thr Ala Cys Ala Gly Ala Gly Ala Ala Cys Gly Gly Gly Gly Gly Thr 
    850                 855                 860                 


Thr Ala Cys Ala Gly Ala Ala Ala Gly Gly Gly Thr Gly Gly Ala Ala 
865                 870                 875                 880 


Ala Cys Gly Ala Ala Cys Gly Thr Cys Gly Gly Ala Gly Gly Ala Gly 
                885                 890                 895     


Gly Ala Ala Thr Gly Ala Gly Gly Ala Gly Ala Gly Ala Gly Gly Ala 
            900                 905                 910         


Gly Gly Ala Ala Ala Gly Ala Gly Gly Ala Gly Gly Thr Ala Gly Ala 
        915                 920                 925             


Thr Cys Gly Ala Gly Cys Ala Gly Ala Thr Thr Gly Ala Thr Gly Thr 
    930                 935                 940                 


Gly Ala Thr Ala Ala Ala Ala Ala Cys Cys Ala Cys Cys Cys Cys Thr 
945                 950                 955                 960 


Cys Cys Ala Ala Ala Ala Cys Thr Gly Thr Thr Ala Thr Thr Ala Thr 
                965                 970                 975     


Gly Cys Cys Cys Cys Cys Ala Ala Gly Thr Thr Thr Ala Cys Gly Ala 
            980                 985                 990         


Thr Thr Thr Thr Thr Cys Thr Cys  Cys Thr Cys Cys Thr  Thr Cys Ala 
        995                 1000                 1005             


Gly Cys  Cys Thr Cys Gly Cys  Thr Cys Gly Cys Thr  Gly Gly Cys 
    1010                 1015                 1020             


Cys Ala  Ala Thr Ala Thr Cys  Thr Thr Thr Gly Ala  Thr Cys Cys 
    1025                 1030                 1035             


Thr Cys  Cys Gly Thr Cys Cys  Ala Thr Gly Gly Ala  Thr Thr Cys 
    1040                 1045                 1050             


Thr Thr  Cys Cys Cys Gly Thr  Thr Cys Cys Gly Cys  Thr Cys Thr 
    1055                 1060                 1065             


Ala Thr  Cys Cys Gly Ala Thr  Ala Ala Gly Ala Gly  Gly Thr Thr 
    1070                 1075                 1080             


Gly Gly  Cys Thr Thr Cys Cys  Gly Ala Gly Ala Ala  Gly Gly Thr 
    1085                 1090                 1095             


Thr Gly  Gly Thr Thr Ala Thr  Thr Gly Gly Ala Gly  Ala Thr Gly 
    1100                 1105                 1110             


Ala Cys  Gly Thr Ala Cys Ala  Ala Ala Gly Thr Gly  Cys Ala Cys 
    1115                 1120                 1125             


Ala Ala  Cys Ala Ala Ala Cys  Cys Ala Gly Ala Thr  Gly Thr Thr 
    1130                 1135                 1140             


Gly Thr  Thr Thr Ala Gly Cys  Gly Cys Gly Ala Thr  Cys Gly Gly 
    1145                 1150                 1155             


Cys Cys  Cys Ala Ala Cys Cys  Cys Cys Thr Cys Thr  Thr Gly Thr 
    1160                 1165                 1170             


Thr Gly  Thr Thr Ala Gly Ala  Gly Ala Gly Thr Cys  Thr Thr Ala 
    1175                 1180                 1185             


Thr Gly  Cys Ala Thr Ala Thr  Thr Thr Cys Gly Thr  Ala Gly Ala 
    1190                 1195                 1200             


Cys Cys  Cys Gly Gly Gly Ala  Cys Thr Gly Thr Ala  Cys Ala Thr 
    1205                 1210                 1215             


Thr Cys  Gly Thr Cys Cys Gly  Thr Cys Cys Thr Cys  Ala Gly Cys 
    1220                 1225                 1230             


Thr Ala  Thr Thr Gly Gly Thr  Cys Ala Thr Thr Thr  Gly Ala Thr 
    1235                 1240                 1245             


Ala Cys  Cys Ala Cys Thr Cys  Ala Ala Ala Gly Gly  Ala Cys Thr 
    1250                 1255                 1260             


Gly Cys  Cys Cys Cys Ala Ala  Cys Cys Cys Thr Gly  Ala Thr Thr 
    1265                 1270                 1275             


Thr Cys  Cys Cys Cys Ala Ala  Ala Cys Ala Gly Cys  Ala Thr Ala 
    1280                 1285                 1290             


Cys Thr  Ala Gly Thr Cys Ala  Thr Thr Cys Ala Thr  Thr Cys Ala 
    1295                 1300                 1305             


Gly Cys  Ala Thr Ala Ala Cys  Gly Gly Gly Gly Thr  Thr Thr Thr 
    1310                 1315                 1320             


Thr Thr  Thr Thr Gly Thr Thr  Thr Cys Ala Ala Cys  Cys Cys Thr 
    1325                 1330                 1335             


Cys Cys  Cys Ala Thr Thr Ala  Thr Ala Thr Ala Thr  Ala Ala Thr 
    1340                 1345                 1350             


Cys Ala  Ala Gly Gly Ala Ala  Gly Cys Cys Cys Thr  Cys Cys Thr 
    1355                 1360                 1365             


Gly Gly  Cys Ala Cys Cys Thr  Thr Cys Cys Cys Thr  Cys Thr Cys 
    1370                 1375                 1380             


Thr Thr  Cys Thr Thr Cys Cys  Cys Thr Cys Thr Cys  Thr Ala Thr 
    1385                 1390                 1395             


Ala Ala  Cys Cys Ala Thr Thr  Thr Cys Ala Cys Thr  Cys Thr Gly 
    1400                 1405                 1410             


Ala Gly  Thr Thr Gly Cys Thr  Thr Thr Ala Thr Ala  Ala Ala Cys 
    1415                 1420                 1425             


Ala Thr  Ala Gly Ala Thr Thr  Ala Cys Gly Thr Cys  Gly Cys Ala 
    1430                 1435                 1440             


Gly Cys  Ala Gly Ala Cys Ala  Cys Thr Cys Ala Cys  Gly Cys Ala 
    1445                 1450                 1455             


Cys Cys  Ala Cys Cys Cys Cys  Cys Thr Cys Thr Thr  Thr Cys Cys 
    1460                 1465                 1470             


Cys Cys  Thr Gly Cys Thr Cys  Thr Cys Ala Ala Ala  Gly Ala Thr 
    1475                 1480                 1485             


Thr Cys  Thr Ala Thr Ala Thr  Ala Ala Cys Ala Gly  Cys Cys Ala 
    1490                 1495                 1500             


<210>  35
<211>  220
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P. pastoris MXR1 protein sequence with S. cerevisiae SV40 nuclear
       localisation signal

<400>  35

Met Ser Gln Lys Arg Pro Thr Ser Leu Arg Phe Ile Thr Ser Phe Pro 
1               5                   10                  15      


Arg Phe Pro Lys Arg Ser Phe Phe Thr Met Pro Val Val Ser Ser Leu 
            20                  25                  30          


Ile Ser Ser Asn Ile Lys Lys Ser Pro Gln Asp Lys Val Val Thr Val 
        35                  40                  45              


Ala Gly Gly Cys Phe Trp Gly Leu Glu Tyr Ile Tyr Lys Met His Phe 
    50                  55                  60                  


Lys Asp Arg Ile Val Asp Thr Gln Val Gly Phe Ala Asn Gly Asn Leu 
65                  70                  75                  80  


Ser Asn Pro Thr Tyr Lys Glu Val Cys Gln Gly Leu Thr Tyr His Ala 
                85                  90                  95      


Glu Val Leu Gln Ile Ala Tyr Asn Pro Glu Val Ile Ser Tyr Lys Glu 
            100                 105                 110         


Leu Ile Asp Phe Phe Phe Leu Val His Asp Pro Thr Gln Asp Asp Gly 
        115                 120                 125             


Gln Gly Pro Asp Ile Gly Thr Gln Tyr Arg Ser Ala Val Phe Tyr Leu 
    130                 135                 140                 


Asp Glu Glu Glu Lys Glu Ile Ala Glu Gln Ser Leu Ala Glu Thr Gln 
145                 150                 155                 160 


Lys Lys Trp Phe Pro His His Glu Ile Val Thr Gln Val Glu Lys Leu 
                165                 170                 175     


Thr Ser Tyr Trp Asp Ala Glu Asp Tyr His Gln Glu Tyr Leu Ile Lys 
            180                 185                 190         


Asn Ala Asp Gly Tyr His Cys Pro Thr His Val Leu Arg Thr Glu Pro 
        195                 200                 205             


Lys Ala Ile Ser Val Pro Lys Lys Lys Arg Lys Val 
    210                 215                 220 


<210>  36
<211>  663
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimised MXR1 DNA sequence

<400>  36
atgtctcaaa aaagaccaac ttctttgaga tttattactt cttttccaag atttccaaaa       60

agatcttttt ttactatgcc agttgtttct tctttgattt cttctaatat taaaaaatct      120

ccacaagata aagttgttac tgttgctggt ggttgttttt ggggtttgga atatatttat      180

aaaatgcatt ttaaagatag aattgttgat actcaagttg gttttgctaa tggtaatttg      240

tctaatccaa cttataaaga agtttgtcaa ggtttgactt atcatgctga agttttgcaa      300

attgcttata atccagaagt tatttcttat aaagaattga ttgatttttt ttttttggtt      360

catgatccaa ctcaagatga tggtcaaggt ccagatattg gtactcaata tagatctgct      420

gttttttatt tggatgaaga agaaaaagaa attgctgaac aatctttggc tgaaactcaa      480

aaaaaatggt ttccacatca tgaaattgtt actcaagttg aaaaattgac ttcttattgg      540

gatgctgaag attatcatca agaatatttg attaaaaatg ctgatggtta tcattgtcca      600

actcatgttt tgagaactga accaaaagct atttctgttc caaagaagaa gagaaaggtt      660

taa                                                                    663


<210>  37
<211>  430
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  37
taagcttgat atcgaattcc tgcagcccgt attctgataa atctaaagag aaattactaa       60

aaaaaagaaa aaaaaaagaa cgggggtgta ataatttgta gttcattatt gcaattatat      120

atctatatct atatatgtat ataacattaa catgtgcatg tacacacgta atcgcgcgtg      180

tacatgtcta tatgtgttac ttgaactata ctgttttgac gtgtatgttt atttatctct      240

cttctgattc ctccacccct tccttactca accgggtaaa tgtcgcatca tgactcccga      300

caataatccc ctctggtata gcgagaagca actttagctt cttaacggca agaacttttt      360

tatgtttgtc gcacctgtat cttcacaaaa gttggataca gcaataagaa aggaaaccac      420

atttgtgcca                                                             430


<210>  38
<211>  386
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  38

Met Ser Ala Ala Thr Val Gly Lys Pro Ile Lys Cys Ile Ala Ala Val 
1               5                   10                  15      


Ala Tyr Asp Ala Lys Lys Pro Leu Ser Val Glu Glu Ile Thr Val Asp 
            20                  25                  30          


Ala Pro Lys Ala His Glu Val Arg Ile Lys Ile Glu Tyr Thr Ala Val 
        35                  40                  45              


Cys His Thr Asp Ala Tyr Thr Leu Ser Gly Ser Asp Pro Glu Gly Leu 
    50                  55                  60                  


Phe Pro Cys Val Leu Gly His Glu Gly Ala Gly Ile Val Glu Ser Val 
65                  70                  75                  80  


Gly Asp Asp Val Ile Thr Val Lys Pro Gly Asp His Val Ile Ala Leu 
                85                  90                  95      


Tyr Thr Ala Glu Cys Gly Lys Cys Lys Phe Cys Thr Ser Gly Lys Thr 
            100                 105                 110         


Asn Leu Cys Gly Ala Val Arg Ala Thr Gln Gly Lys Gly Val Met Pro 
        115                 120                 125             


Asp Gly Thr Thr Arg Phe His Asn Ala Lys Gly Glu Asp Ile Tyr His 
    130                 135                 140                 


Phe Met Gly Cys Ser Thr Phe Ser Glu Tyr Thr Val Val Ala Asp Val 
145                 150                 155                 160 


Ser Val Val Ala Ile Asp Pro Lys Ala Pro Leu Asp Ala Ala Cys Leu 
                165                 170                 175     


Leu Gly Cys Gly Val Thr Thr Gly Phe Gly Ala Ala Leu Lys Thr Ala 
            180                 185                 190         


Asn Val Gln Lys Gly Asp Thr Val Ala Val Phe Gly Cys Gly Thr Val 
        195                 200                 205             


Gly Leu Ser Val Ile Gln Gly Ala Lys Leu Arg Gly Ala Ser Lys Ile 
    210                 215                 220                 


Ile Ala Ile Asp Ile Asn Asn Lys Lys Lys Gln Tyr Cys Ser Gln Phe 
225                 230                 235                 240 


Gly Ala Thr Asp Phe Val Asn Pro Lys Glu Asp Leu Ala Lys Asp Gln 
                245                 250                 255     


Thr Ile Val Glu Lys Leu Ile Glu Met Thr Asp Gly Gly Leu Asp Phe 
            260                 265                 270         


Thr Phe Asp Cys Thr Gly Asn Thr Lys Ile Met Arg Asp Ala Leu Glu 
        275                 280                 285             


Ala Cys His Lys Gly Trp Gly Gln Ser Ile Ile Ile Gly Val Ala Ala 
    290                 295                 300                 


Ala Gly Glu Glu Ile Ser Thr Arg Pro Phe Gln Leu Val Thr Gly Arg 
305                 310                 315                 320 


Val Trp Lys Gly Ser Ala Phe Gly Gly Ile Lys Gly Arg Ser Glu Met 
                325                 330                 335     


Gly Gly Leu Ile Lys Asp Tyr Gln Lys Gly Ala Leu Lys Val Glu Glu 
            340                 345                 350         


Phe Ile Thr His Arg Arg Pro Phe Lys Glu Ile Asn Gln Ala Phe Glu 
        355                 360                 365             


Asp Leu His Asn Gly Asp Cys Leu Arg Thr Val Leu Lys Ser Asp Glu 
    370                 375                 380                 


Ile Lys 
385     


<210>  39
<211>  1161
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  39
atgtccgccg ctactgttgg taaacctatt aagtgcattg ctgctgttgc gtatgatgcg       60

aagaaaccat taagtgttga agaaatcacg gtagacgccc caaaagcgca cgaagtacgt      120

atcaaaattg aatatactgc tgtatgccac actgatgcgt acactttatc aggctctgat      180

ccagaaggac ttttcccttg cgttctgggc cacgaaggag ccggtatcgt agaatctgta      240

ggcgatgatg tcataacagt taagcctggt gatcatgtta ttgctttgta cactgctgag      300

tgtggcaaat gtaagttctg tacttccggt aaaaccaact tatgtggtgc tgttagagct      360

actcaaggga aaggtgtaat gcctgatggg accacaagat ttcataatgc gaaaggtgaa      420

gatatatacc atttcatggg ttgctctact ttttccgaat atactgtggt ggcagatgtc      480

tctgtggttg ccatcgatcc aaaagctccc ttggatgctg cctgtttact gggttgtggt      540

gttactactg gttttggggc ggctcttaag acagctaatg tgcaaaaagg cgataccgtt      600

gcagtatttg gctgcgggac tgtaggactc tccgttatcc aaggtgcaaa gttaaggggc      660

gcttccaaga tcattgccat tgacattaac aataagaaaa aacaatattg ttctcaattt      720

ggtgccacgg attttgttaa tcccaaggaa gatttggcca aagatcaaac tatcgttgaa      780

aagttaattg aaatgactga tgggggtctg gattttactt ttgactgtac tggtaatacc      840

aaaattatga gagatgcttt ggaagcctgt cataaaggtt ggggtcaatc tattatcatt      900

ggtgtggctg ccgctggtga agaaatttct acaaggccgt tccagctggt cactggtaga      960

gtgtggaaag gctctgcttt tggtggcatc aaaggtagat ctgaaatggg cggtttaatt     1020

aaagactatc aaaaaggtgc cttaaaagtc gaagaattta tcactcacag gagaccattc     1080

aaagaaatca atcaagcctt tgaagatttg cataacggtg attgcttaag aaccgtcttg     1140

aagtctgatg aaataaaata g                                               1161


