                         SEQUENCE LISTING

<110>  THOENE, Jess G.
 
<120>  TREATMENT OF CYSTIC FIBROSIS VIA MICROVESICLE-MEDIATED CFTR REPLACEMENT

<130>  500135US

<140>  PCT/US18/13070
<141>  2018-01-10

<150>  US 62/446,162
<151>  2017-01-13

<160>  1

<170>  PatentIn version 3.5

<210>  1
<211>  1480
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Gln Arg Ser Pro Leu Glu Lys Ala Ser Val Val Ser Lys Leu Phe 
1               5                   10                  15      


Phe Ser Trp Thr Arg Pro Ile Leu Arg Lys Gly Tyr Arg Gln Arg Leu 
            20                  25                  30          


Glu Leu Ser Asp Ile Tyr Gln Ile Pro Ser Val Asp Ser Ala Asp Asn 
        35                  40                  45              


Leu Ser Glu Lys Leu Glu Arg Glu Trp Asp Arg Glu Leu Ala Ser Lys 
    50                  55                  60                  


Lys Asn Pro Lys Leu Ile Asn Ala Leu Arg Arg Cys Phe Phe Trp Arg 
65                  70                  75                  80  


Phe Met Phe Tyr Gly Ile Phe Leu Tyr Leu Gly Glu Val Thr Lys Ala 
                85                  90                  95      


Val Gln Pro Leu Leu Leu Gly Arg Ile Ile Ala Ser Tyr Asp Pro Asp 
            100                 105                 110         


Asn Lys Glu Glu Arg Ser Ile Ala Ile Tyr Leu Gly Ile Gly Leu Cys 
        115                 120                 125             


Leu Leu Phe Ile Val Arg Thr Leu Leu Leu His Pro Ala Ile Phe Gly 
    130                 135                 140                 


Leu His His Ile Gly Met Gln Met Arg Ile Ala Met Phe Ser Leu Ile 
145                 150                 155                 160 


Tyr Lys Lys Thr Leu Lys Leu Ser Ser Arg Val Leu Asp Lys Ile Ser 
                165                 170                 175     


Ile Gly Gln Leu Val Ser Leu Leu Ser Asn Asn Leu Asn Lys Phe Asp 
            180                 185                 190         


Glu Gly Leu Ala Leu Ala His Phe Val Trp Ile Ala Pro Leu Gln Val 
        195                 200                 205             


Ala Leu Leu Met Gly Leu Ile Trp Glu Leu Leu Gln Ala Ser Ala Phe 
    210                 215                 220                 


Cys Gly Leu Gly Phe Leu Ile Val Leu Ala Leu Phe Gln Ala Gly Leu 
225                 230                 235                 240 


Gly Arg Met Met Met Lys Tyr Arg Asp Gln Arg Ala Gly Lys Ile Ser 
                245                 250                 255     


Glu Arg Leu Val Ile Thr Ser Glu Met Ile Glu Asn Ile Gln Ser Val 
            260                 265                 270         


Lys Ala Tyr Cys Trp Glu Glu Ala Met Glu Lys Met Ile Glu Asn Leu 
        275                 280                 285             


Arg Gln Thr Glu Leu Lys Leu Thr Arg Lys Ala Ala Tyr Val Arg Tyr 
    290                 295                 300                 


Phe Asn Ser Ser Ala Phe Phe Phe Ser Gly Phe Phe Val Val Phe Leu 
305                 310                 315                 320 


Ser Val Leu Pro Tyr Ala Leu Ile Lys Gly Ile Ile Leu Arg Lys Ile 
                325                 330                 335     


Phe Thr Thr Ile Ser Phe Cys Ile Val Leu Arg Met Ala Val Thr Arg 
            340                 345                 350         


Gln Phe Pro Trp Ala Val Gln Thr Trp Tyr Asp Ser Leu Gly Ala Ile 
        355                 360                 365             


Asn Lys Ile Gln Asp Phe Leu Gln Lys Gln Glu Tyr Lys Thr Leu Glu 
    370                 375                 380                 


Tyr Asn Leu Thr Thr Thr Glu Val Val Met Glu Asn Val Thr Ala Phe 
385                 390                 395                 400 


Trp Glu Glu Gly Phe Gly Glu Leu Phe Glu Lys Ala Lys Gln Asn Asn 
                405                 410                 415     


Asn Asn Arg Lys Thr Ser Asn Gly Asp Asp Ser Leu Phe Phe Ser Asn 
            420                 425                 430         


Phe Ser Leu Leu Gly Thr Pro Val Leu Lys Asp Ile Asn Phe Lys Ile 
        435                 440                 445             


Glu Arg Gly Gln Leu Leu Ala Val Ala Gly Ser Thr Gly Ala Gly Lys 
    450                 455                 460                 


Thr Ser Leu Leu Met Val Ile Met Gly Glu Leu Glu Pro Ser Glu Gly 
465                 470                 475                 480 


Lys Ile Lys His Ser Gly Arg Ile Ser Phe Cys Ser Gln Phe Ser Trp 
                485                 490                 495     


Ile Met Pro Gly Thr Ile Lys Glu Asn Ile Ile Phe Gly Val Ser Tyr 
            500                 505                 510         


Asp Glu Tyr Arg Tyr Arg Ser Val Ile Lys Ala Cys Gln Leu Glu Glu 
        515                 520                 525             


Asp Ile Ser Lys Phe Ala Glu Lys Asp Asn Ile Val Leu Gly Glu Gly 
    530                 535                 540                 


Gly Ile Thr Leu Ser Gly Gly Gln Arg Ala Arg Ile Ser Leu Ala Arg 
545                 550                 555                 560 


Ala Val Tyr Lys Asp Ala Asp Leu Tyr Leu Leu Asp Ser Pro Phe Gly 
                565                 570                 575     


Tyr Leu Asp Val Leu Thr Glu Lys Glu Ile Phe Glu Ser Cys Val Cys 
            580                 585                 590         


Lys Leu Met Ala Asn Lys Thr Arg Ile Leu Val Thr Ser Lys Met Glu 
        595                 600                 605             


His Leu Lys Lys Ala Asp Lys Ile Leu Ile Leu His Glu Gly Ser Ser 
    610                 615                 620                 


Tyr Phe Tyr Gly Thr Phe Ser Glu Leu Gln Asn Leu Gln Pro Asp Phe 
625                 630                 635                 640 


Ser Ser Lys Leu Met Gly Cys Asp Ser Phe Asp Gln Phe Ser Ala Glu 
                645                 650                 655     


Arg Arg Asn Ser Ile Leu Thr Glu Thr Leu His Arg Phe Ser Leu Glu 
            660                 665                 670         


Gly Asp Ala Pro Val Ser Trp Thr Glu Thr Lys Lys Gln Ser Phe Lys 
        675                 680                 685             


Gln Thr Gly Glu Phe Gly Glu Lys Arg Lys Asn Ser Ile Leu Asn Pro 
    690                 695                 700                 


Ile Asn Ser Ile Arg Lys Phe Ser Ile Val Gln Lys Thr Pro Leu Gln 
705                 710                 715                 720 


Met Asn Gly Ile Glu Glu Asp Ser Asp Glu Pro Leu Glu Arg Arg Leu 
                725                 730                 735     


Ser Leu Val Pro Asp Ser Glu Gln Gly Glu Ala Ile Leu Pro Arg Ile 
            740                 745                 750         


Ser Val Ile Ser Thr Gly Pro Thr Leu Gln Ala Arg Arg Arg Gln Ser 
        755                 760                 765             


Val Leu Asn Leu Met Thr His Ser Val Asn Gln Gly Gln Asn Ile His 
    770                 775                 780                 


Arg Lys Thr Thr Ala Ser Thr Arg Lys Val Ser Leu Ala Pro Gln Ala 
785                 790                 795                 800 


Asn Leu Thr Glu Leu Asp Ile Tyr Ser Arg Arg Leu Ser Gln Glu Thr 
                805                 810                 815     


Gly Leu Glu Ile Ser Glu Glu Ile Asn Glu Glu Asp Leu Lys Glu Cys 
            820                 825                 830         


Phe Phe Asp Asp Met Glu Ser Ile Pro Ala Val Thr Thr Trp Asn Thr 
        835                 840                 845             


Tyr Leu Arg Tyr Ile Thr Val His Lys Ser Leu Ile Phe Val Leu Ile 
    850                 855                 860                 


Trp Cys Leu Val Ile Phe Leu Ala Glu Val Ala Ala Ser Leu Val Val 
865                 870                 875                 880 


Leu Trp Leu Leu Gly Asn Thr Pro Leu Gln Asp Lys Gly Asn Ser Thr 
                885                 890                 895     


His Ser Arg Asn Asn Ser Tyr Ala Val Ile Ile Thr Ser Thr Ser Ser 
            900                 905                 910         


Tyr Tyr Val Phe Tyr Ile Tyr Val Gly Val Ala Asp Thr Leu Leu Ala 
        915                 920                 925             


Met Gly Phe Phe Arg Gly Leu Pro Leu Val His Thr Leu Ile Thr Val 
    930                 935                 940                 


Ser Lys Ile Leu His His Lys Met Leu His Ser Val Leu Gln Ala Pro 
945                 950                 955                 960 


Met Ser Thr Leu Asn Thr Leu Lys Ala Gly Gly Ile Leu Asn Arg Phe 
                965                 970                 975     


Ser Lys Asp Ile Ala Ile Leu Asp Asp Leu Leu Pro Leu Thr Ile Phe 
            980                 985                 990         


Asp Phe Ile Gln Leu Leu Leu Ile Val Ile Gly Ala Ile Ala Val Val 
        995                 1000                1005            


Ala Val Leu Gln Pro Tyr Ile Phe Val Ala Thr Val Pro Val Ile Val 
    1010                1015                1020                


Ala Phe Ile Met Leu Arg Ala Tyr Phe Leu Gln Thr Ser Gln Gln Leu 
1025                1030                1035                1040


Lys Gln Leu Glu Ser Glu Gly Arg Ser Pro Ile Phe Thr His Leu Val 
                1045                1050                1055    


Thr Ser Leu Lys Gly Leu Trp Thr Leu Arg Ala Phe Gly Arg Gln Pro 
            1060                1065                1070        


Tyr Phe Glu Thr Leu Phe His Lys Ala Leu Asn Leu His Thr Ala Asn 
        1075                1080                1085            


Trp Phe Leu Tyr Leu Ser Thr Leu Arg Trp Phe Gln Met Arg Ile Glu 
    1090                1095                1100                


Met Ile Phe Val Ile Phe Phe Ile Ala Val Thr Phe Ile Ser Ile Leu 
1105                1110                1115                1120


Thr Thr Gly Glu Gly Glu Gly Arg Val Gly Ile Ile Leu Thr Leu Ala 
                1125                1130                1135    


Met Asn Ile Met Ser Thr Leu Gln Trp Ala Val Asn Ser Ser Ile Asp 
            1140                1145                1150        


Val Asp Ser Leu Met Arg Ser Val Ser Arg Val Phe Lys Phe Ile Asp 
        1155                1160                1165            


Met Pro Thr Glu Gly Lys Pro Thr Lys Ser Thr Lys Pro Tyr Lys Asn 
    1170                1175                1180                


Gly Gln Leu Ser Lys Val Met Ile Ile Glu Asn Ser His Val Lys Lys 
1185                1190                1195                1200


Asp Asp Ile Trp Pro Ser Gly Gly Gln Met Thr Val Lys Asp Leu Thr 
                1205                1210                1215    


Ala Lys Tyr Thr Glu Gly Gly Asn Ala Ile Leu Glu Asn Ile Ser Phe 
            1220                1225                1230        


Ser Ile Ser Pro Gly Gln Arg Val Gly Leu Leu Gly Arg Thr Gly Ser 
        1235                1240                1245            


Gly Lys Ser Thr Leu Leu Ser Ala Phe Leu Arg Leu Leu Asn Thr Glu 
    1250                1255                1260                


Gly Glu Ile Gln Ile Asp Gly Val Ser Trp Asp Ser Ile Thr Leu Gln 
1265                1270                1275                1280


Gln Trp Arg Lys Ala Phe Gly Val Ile Pro Gln Lys Val Phe Ile Phe 
                1285                1290                1295    


Ser Gly Thr Phe Arg Lys Asn Leu Asp Pro Tyr Glu Gln Trp Ser Asp 
            1300                1305                1310        


Gln Glu Ile Trp Lys Val Ala Asp Glu Val Gly Leu Arg Ser Val Ile 
        1315                1320                1325            


Glu Gln Phe Pro Gly Lys Leu Asp Phe Val Leu Val Asp Gly Gly Cys 
    1330                1335                1340                


Val Leu Ser His Gly His Lys Gln Leu Met Cys Leu Ala Arg Ser Val 
1345                1350                1355                1360


Leu Ser Lys Ala Lys Ile Leu Leu Leu Asp Glu Pro Ser Ala His Leu 
                1365                1370                1375    


Asp Pro Val Thr Tyr Gln Ile Ile Arg Arg Thr Leu Lys Gln Ala Phe 
            1380                1385                1390        


Ala Asp Cys Thr Val Ile Leu Cys Glu His Arg Ile Glu Ala Met Leu 
        1395                1400                1405            


Glu Cys Gln Gln Phe Leu Val Ile Glu Glu Asn Lys Val Arg Gln Tyr 
    1410                1415                1420                


Asp Ser Ile Gln Lys Leu Leu Asn Glu Arg Ser Leu Phe Arg Gln Ala 
1425                1430                1435                1440


Ile Ser Pro Ser Asp Arg Val Lys Leu Phe Pro His Arg Asn Ser Ser 
                1445                1450                1455    


Lys Cys Lys Ser Lys Pro Gln Ile Ala Ala Leu Lys Glu Glu Thr Glu 
            1460                1465                1470        


Glu Glu Val Gln Asp Thr Arg Leu 
        1475                1480

