                         SEQUENCE LISTING

<110>  Protalix Ltd.
       Almon, Einat
       Chertkoff, Raul
       Alon, Sari
       Shaaltiel, Yosef
 
<120>  THERAPEUTIC REGIMEN FOR THE TREATMENT OF FABRY USING STABILIZED 
       ALPHA-GALACTOSIDASE

<130>  72305

<150>  US 62/442,537
<151>  2017-01-05

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  398
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Human mature Alpha galactosidase protein sequence

<400>  1

Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      


Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          


Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              


Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  


Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  


Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      


Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         


Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             


Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 


Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 


Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     


Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         


Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             


Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 


Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 


Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     


Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         


Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             


Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 


Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 


Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     


Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         


Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             


Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 


Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu 
385                 390                 395             


<210>  2
<211>  405
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant A-Gal mature protein with N' terminal G and fused to 
       SEKDEL

<400>  2

Gly Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His 
1               5                   10                  15      


Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser 
            20                  25                  30          


Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser 
        35                  40                  45              


Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys 
    50                  55                  60                  


Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro 
65                  70                  75                  80  


Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser 
                85                  90                  95      


Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys 
            100                 105                 110         


Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr 
        115                 120                 125             


Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys 
    130                 135                 140                 


Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala 
145                 150                 155                 160 


Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu 
                165                 170                 175     


Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr 
            180                 185                 190         


Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser 
        195                 200                 205             


Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val 
    210                 215                 220                 


Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile 
225                 230                 235                 240 


Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu 
                245                 250                 255     


Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His 
            260                 265                 270         


Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala 
        275                 280                 285             


Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly 
    290                 295                 300                 


Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala 
305                 310                 315                 320 


Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr 
                325                 330                 335     


Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys 
            340                 345                 350         


Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu 
        355                 360                 365             


Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu 
    370                 375                 380                 


Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu Ser 
385                 390                 395                 400 


Glu Lys Asp Glu Leu 
                405 


<210>  3
<211>  404
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant A-Gal mature protein fused to SEKDEL without N' 
       terminal G

<400>  3

Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      


Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          


Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              


Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  


Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  


Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      


Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         


Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             


Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 


Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 


Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     


Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         


Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             


Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 


Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 


Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     


Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         


Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             


Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 


Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 


Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     


Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         


Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             


Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 


Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu Ser Glu 
385                 390                 395                 400 


Lys Asp Glu Leu 
                


