               SEQUENCE LISTING

<110> EvolVeritas Kft.

<120> Novel proteins and use thereof

<130> 15-120-HU

<160> 36

<170> BiSSAP 1.3.6

<210> 1
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Designed peptide to act as the inhibitory loop in a protein

<400> 1
Phe Cys Arg Ala Val Lys Arg 
1               5           

<210> 2
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Designed peptide to act as the inhibitory loop in a protein

<400> 2
Tyr Cys Arg Ala Val Lys Arg 
1               5           

<210> 3
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Designed peptide to act as the inhibitory loop in a protein

<400> 3
Leu Cys Arg Ala Val Lys Arg 
1               5           

<210> 4
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Designed peptide to act as the inhibitory loop in a protein

<400> 4
Pro Cys Arg Ala Val Lys Arg 
1               5           

<210> 5
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Designed peptide to act as the inhibitory loop in a protein

<400> 5
Gln Cys Arg Ala Val Lys Arg 
1               5           

<210> 6
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Designed peptide to act as the inhibitory loop in a protein

<400> 6
Met Cys Arg Ala Val Lys Arg 
1               5           

<210> 7
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Designed peptide to act as the inhibitory loop in a protein

<400> 7
Val Cys Arg Ala Val Lys Arg 
1               5           

<210> 8
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Designed peptide to act as the inhibitory loop in a protein

<400> 8
Trp Cys Arg Ala Val Lys Arg 
1               5           

<210> 9
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Designed peptide to act as the inhibitory loop in a protein

<400> 9
Pro Cys Arg Ala Leu Lys Ile 
1               5           

<210> 10
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Designed peptide to act as the inhibitory loop in a protein

<400> 10
Pro Cys Arg Ala Val Lys Ile 
1               5           

<210> 11
<211> 58
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified Kunitz domain protein

<400> 11
Lys Pro Asp Phe Cys Phe Leu Glu Glu Asp Pro Gly Phe Cys Arg Ala 
1               5                   10                  15      
Val Lys Arg Arg Tyr Phe Tyr Asn Asn Gln Thr Lys Gln Cys Glu Arg 
            20                  25                  30          
Phe Lys Tyr Gly Gly Cys Leu Gly Asn Met Asn Asn Phe Glu Thr Leu 
        35                  40                  45              
Glu Glu Cys Lys Asn Ile Cys Glu Asp Gly 
    50                  55              

<210> 12
<211> 58
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified Kunitz domain protein

<400> 12
Lys Pro Asp Phe Cys Phe Leu Glu Glu Asp Pro Gly Tyr Cys Arg Ala 
1               5                   10                  15      
Val Lys Arg Arg Tyr Phe Tyr Asn Asn Gln Thr Lys Gln Cys Glu Arg 
            20                  25                  30          
Phe Lys Tyr Gly Gly Cys Leu Gly Asn Met Asn Asn Phe Glu Thr Leu 
        35                  40                  45              
Glu Glu Cys Lys Asn Ile Cys Glu Asp Gly 
    50                  55              

<210> 13
<211> 58
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified Kunitz domain protein

<400> 13
Lys Pro Asp Phe Cys Phe Leu Glu Glu Asp Pro Gly Leu Cys Arg Ala 
1               5                   10                  15      
Val Lys Arg Arg Tyr Phe Tyr Asn Asn Gln Thr Lys Gln Cys Glu Arg 
            20                  25                  30          
Phe Lys Tyr Gly Gly Cys Leu Gly Asn Met Asn Asn Phe Glu Thr Leu 
        35                  40                  45              
Glu Glu Cys Lys Asn Ile Cys Glu Asp Gly 
    50                  55              

<210> 14
<211> 58
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified Kunitz domain protein

<400> 14
Lys Pro Asp Phe Cys Phe Leu Glu Glu Asp Pro Gly Pro Cys Arg Ala 
1               5                   10                  15      
Val Lys Arg Arg Tyr Phe Tyr Asn Asn Gln Thr Lys Gln Cys Glu Arg 
            20                  25                  30          
Phe Lys Tyr Gly Gly Cys Leu Gly Asn Met Asn Asn Phe Glu Thr Leu 
        35                  40                  45              
Glu Glu Cys Lys Asn Ile Cys Glu Asp Gly 
    50                  55              

<210> 15
<211> 58
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified Kunitz domain protein

<400> 15
Lys Pro Asp Phe Cys Phe Leu Glu Glu Asp Pro Gly Gln Cys Arg Ala 
1               5                   10                  15      
Val Lys Arg Arg Tyr Phe Tyr Asn Asn Gln Thr Lys Gln Cys Glu Arg 
            20                  25                  30          
Phe Lys Tyr Gly Gly Cys Leu Gly Asn Met Asn Asn Phe Glu Thr Leu 
        35                  40                  45              
Glu Glu Cys Lys Asn Ile Cys Glu Asp Gly 
    50                  55              

<210> 16
<211> 58
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified Kunitz domain protein

<400> 16
Lys Pro Asp Phe Cys Phe Leu Glu Glu Asp Pro Gly Met Cys Arg Ala 
1               5                   10                  15      
Val Lys Arg Arg Tyr Phe Tyr Asn Asn Gln Thr Lys Gln Cys Glu Arg 
            20                  25                  30          
Phe Lys Tyr Gly Gly Cys Leu Gly Asn Met Asn Asn Phe Glu Thr Leu 
        35                  40                  45              
Glu Glu Cys Lys Asn Ile Cys Glu Asp Gly 
    50                  55              

<210> 17
<211> 58
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified Kunitz domain protein

<400> 17
Lys Pro Asp Phe Cys Phe Leu Glu Glu Asp Pro Gly Val Cys Arg Ala 
1               5                   10                  15      
Val Lys Arg Arg Tyr Phe Tyr Asn Asn Gln Thr Lys Gln Cys Glu Arg 
            20                  25                  30          
Phe Lys Tyr Gly Gly Cys Leu Gly Asn Met Asn Asn Phe Glu Thr Leu 
        35                  40                  45              
Glu Glu Cys Lys Asn Ile Cys Glu Asp Gly 
    50                  55              

<210> 18
<211> 58
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified Kunitz domain protein

<400> 18
Lys Pro Asp Phe Cys Phe Leu Glu Glu Asp Pro Gly Trp Cys Arg Ala 
1               5                   10                  15      
Val Lys Arg Arg Tyr Phe Tyr Asn Asn Gln Thr Lys Gln Cys Glu Arg 
            20                  25                  30          
Phe Lys Tyr Gly Gly Cys Leu Gly Asn Met Asn Asn Phe Glu Thr Leu 
        35                  40                  45              
Glu Glu Cys Lys Asn Ile Cys Glu Asp Gly 
    50                  55              

<210> 19
<211> 58
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified Kunitz domain protein

<400> 19
Lys Pro Asp Phe Cys Phe Leu Glu Glu Asp Pro Gly Pro Cys Arg Ala 
1               5                   10                  15      
Leu Lys Ile Arg Tyr Phe Tyr Asn Asn Gln Thr Lys Gln Cys Glu Arg 
            20                  25                  30          
Phe Lys Tyr Gly Gly Cys Leu Gly Asn Met Asn Asn Phe Glu Thr Leu 
        35                  40                  45              
Glu Glu Cys Lys Asn Ile Cys Glu Asp Gly 
    50                  55              

<210> 20
<211> 58
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified Kunitz domain protein

<400> 20
Lys Pro Asp Phe Cys Phe Leu Glu Glu Asp Pro Gly Pro Cys Arg Ala 
1               5                   10                  15      
Val Lys Ile Arg Tyr Phe Tyr Asn Asn Gln Thr Lys Gln Cys Glu Arg 
            20                  25                  30          
Phe Lys Tyr Gly Gly Cys Leu Gly Asn Met Asn Asn Phe Glu Thr Leu 
        35                  40                  45              
Glu Glu Cys Lys Asn Ile Cys Glu Asp Gly 
    50                  55              

<210> 21
<211> 58
<212> PRT
<213> Bovidae


<220> 
<223> Bovine pancreatic trypsin inhibitor

<400> 21
Arg Pro Asp Phe Cys Leu Glu Pro Pro Tyr Thr Gly Pro Cys Lys Ala 
1               5                   10                  15      
Arg Ile Ile Arg Tyr Phe Tyr Asn Ala Lys Ala Gly Leu Cys Gln Thr 
            20                  25                  30          
Phe Val Tyr Gly Gly Cys Arg Ala Lys Arg Asn Met Phe Lys Ser Ala 
        35                  40                  45              
Glu Asp Cys Met Arg Thr Cys Gly Gly Ala 
    50                  55              

<210> 22
<211> 58
<212> PRT
<213> Artificial Sequence


<220> 
<223> General sequence of the Kunitz domains

<400> 22
Xaa Xaa Xaa Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Gly Xaa Cys Xaa Xaa 
1               5                   10                  15      
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Cys Xaa Xaa 
            20                  25                  30          
Phe Xaa Xaa Xaa Gly Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              
Xaa Xaa Cys Xaa Xaa Xaa Cys Xaa Xaa Xaa 
    50                  55              

<210> 23
<211> 58
<212> PRT
<213> Homo sapiens


<220> 
<223> Tissue factor pathway inhibitor (UniProt ID P10646) domain 2

<400> 23
Lys Pro Asp Phe Cys Phe Leu Glu Glu Asp Pro Gly Ile Cys Arg Gly 
1               5                   10                  15      
Tyr Ile Thr Arg Tyr Phe Tyr Asn Asn Gln Thr Lys Gln Cys Glu Arg 
            20                  25                  30          
Phe Lys Tyr Gly Gly Cys Leu Gly Asn Met Asn Asn Phe Glu Thr Leu 
        35                  40                  45              
Glu Glu Cys Lys Asn Ile Cys Glu Asp Gly 
    50                  55              

<210> 24
<211> 218
<212> DNA
<213> Artificial Sequence


<220> 
<223> Codon optimized gene for the 2nd domain of the Tissue Factor
      Pathway Inhibitor-1 protein, supplied it with the necessary
      flanking regions for cloning into the display vector between
      Kpn2I (BspEI) and SacI sites

<400> 24
tccggaggct cgggcaaacc ggacttctgc ttcctggaag aagacccggg tatctgccgt     60

ggttacatca cccgttactt ctacaacaac cagaccaaac agtgcgaacg tttcaaatac    120

ggtggttgcc tgggtaacat gaacaacttc gaaaccctgg aagaatgcaa aaacatctgc    180

gaagacggtg gcggcagcgg cggcagcggc gggagctc                            218


<210> 25
<211> 64
<212> DNA
<213> Artificial Sequence


<220> 
<223> TFPI D2 P3-P4' STOP oligonucleotide

<400> 25
cctggaagaa gacccgggtt aatgctaata ataataataa cgttacttct acaacaacca     60

gacc                                                                  64


<210> 26
<211> 64
<212> DNA
<213> Artificial Sequence


<220> 
<223> TFPI D2 P3-P4' library primer

<400> 26
cctggaagaa gacccgggtn nktgcnnknn knnknnknnk cgttacttct acaacaacca     60

gacc                                                                  64


<210> 27
<211> 390
<212> PRT
<213> Rattus


<220> 
<223> Catalytic fragment of rat MASP-2 (MASP2cf)

<400> 27
Met Thr Gln Pro Cys Pro Asp Pro Thr Ala Pro Pro Asn Gly His Ile 
1               5                   10                  15      
Ser Pro Val Gln Ala Thr Tyr Val Leu Lys Asp Ser Phe Ser Val Phe 
            20                  25                  30          
Cys Lys Thr Gly Phe Glu Leu Leu Gln Gly Ser Val Pro Leu Lys Ser 
        35                  40                  45              
Phe Thr Ala Val Cys Gln Lys Asp Gly Ser Trp Asp Arg Pro Ile Pro 
    50                  55                  60                  
Glu Cys Ser Ile Ile Asp Cys Gly Pro Pro Asp Asp Leu Pro Asn Gly 
65                  70                  75                  80  
His Val Asp Tyr Ile Thr Gly Pro Glu Val Thr Thr Tyr Lys Ala Val 
                85                  90                  95      
Ile Gln Tyr Ser Cys Glu Glu Thr Phe Tyr Thr Met Ser Ser Asn Gly 
            100                 105                 110         
Lys Tyr Val Cys Glu Ala Asp Gly Phe Trp Thr Ser Ser Lys Gly Glu 
        115                 120                 125             
Lys Ser Leu Pro Val Cys Lys Pro Val Cys Gly Leu Ser Thr His Thr 
    130                 135                 140                 
Ser Gly Gly Arg Ile Ile Gly Gly Gln Pro Ala Lys Pro Gly Asp Phe 
145                 150                 155                 160 
Pro Trp Gln Val Leu Leu Leu Gly Glu Thr Thr Ala Ala Gly Ala Leu 
                165                 170                 175     
Ile His Asp Asp Trp Val Leu Thr Ala Ala His Ala Val Tyr Gly Lys 
            180                 185                 190         
Thr Glu Ala Met Ser Ser Leu Asp Ile Arg Met Gly Ile Leu Lys Arg 
        195                 200                 205             
Leu Ser Pro His Tyr Thr Gln Ala Trp Pro Glu Ala Val Phe Ile His 
    210                 215                 220                 
Glu Gly Tyr Thr His Gly Ala Gly Phe Asp Asn Asp Ile Ala Leu Ile 
225                 230                 235                 240 
Lys Leu Lys Asn Lys Val Thr Ile Asn Arg Asn Ile Met Pro Ile Cys 
                245                 250                 255     
Leu Pro Arg Lys Glu Ala Ala Ser Leu Met Lys Thr Asp Phe Val Gly 
            260                 265                 270         
Thr Val Ala Gly Trp Gly Leu Thr Gln Lys Gly Phe Leu Ala Arg Asn 
        275                 280                 285             
Leu Met Phe Val Asp Ile Pro Ile Val Asp His Gln Lys Cys Ala Thr 
    290                 295                 300                 
Ala Tyr Thr Lys Gln Pro Tyr Pro Gly Ala Lys Val Thr Val Asn Met 
305                 310                 315                 320 
Leu Cys Ala Gly Leu Asp Ala Gly Gly Lys Asp Ser Cys Arg Gly Asp 
                325                 330                 335     
Ser Gly Gly Ala Leu Val Phe Leu Asp Asn Glu Thr Gln Arg Trp Phe 
            340                 345                 350         
Val Gly Gly Ile Val Ser Trp Gly Ser Ile Asn Cys Gly Gly Ser Glu 
        355                 360                 365             
Gln Tyr Gly Val Tyr Thr Lys Val Thr Asn Tyr Ile Pro Trp Ile Glu 
    370                 375                 380                 
Asn Ile Ile Asn Asn Phe 
385                 390 

<210> 28
<211> 410
<212> DNA
<213> Artificial Sequence


<220> 
<223> Synthetic gene

<400> 28
ccatgggcca tcaccatcac catcacgcta gcatggcgtc ccctctggag aaggccctgg      60

atgtgatggt gtccaccttc cacaagtact cgggcaaaga gggtgacaag ttcaagctca     120

acaagtcaga actaaaggag ctgctgaccc gggagctgcc cagcttcttg gggaaaagga     180

cagatgaagc tgctttccag aagctgatga gcaacttgga cagcaacagg gacaacgagg     240

tggacttcca agagtactgt gtcttcctgt cctccatcgc catgatgtct aacgaattct     300

ttgaaggctt cccagataag cagcccagga agaaagtcga cgactacgac atcccgacta     360

ccgaaaacct gtacttccag ggatcccata tgtatggtac ccgactcgag                410


<210> 29
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> TFPI BamHI forward oligonucleotide

<400> 29
agtaggatcc aaaccggact tctgc                                          25


<210> 30
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> TFPI HindIII reverse oligonucleotide

<400> 30
gtataagctt attaaccgtc ttcgcagatg                                     30


<210> 31
<211> 64
<212> DNA
<213> Artificial Sequence


<220> 
<223> Mutagenesis primer for the production of the protein according to
      SEQ ID NO: 11

<400> 31
cctggaagaa gacccgggtt tctgccgtgc ggtgaaacgt cgttacttct acaacaacca     60

gacc                                                                  64


<210> 32
<211> 64
<212> DNA
<213> Artificial Sequence


<220> 
<223> Mutagenesis primer for the production of the protein according to
      SEQ ID NO: 14

<400> 32
cctggaagaa gacccgggtc cgtgccgtgc ggtgaaacgt cgttacttct acaacaacca     60

gacc                                                                  64


<210> 33
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> Mutagenesis primer for the production of the protein according to
      SEQ ID NO: 17

<400> 33
cgggtgtgtg ccgtgcggtg aaacg                                          25


<210> 34
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> S100A4 3' oligonucleotide

<400> 34
ctagtctcga gtgccaagc                                                 19


<210> 35
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> pMal 5' oligonucleotide

<400> 35
gatgtccgct ttctggtatg c                                              21


<210> 36
<211> 38
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic protein of insect origin

<400> 36
Gly Ser Gly Glu Val Thr Cys Glu Pro Gly Thr Thr Phe Lys Asp Lys 
1               5                   10                  15      
Cys Asn Thr Cys Arg Cys Gly Ser Asp Gly Lys Ser Ala Val Cys Thr 
            20                  25                  30          
Lys Leu Trp Cys Asn Gln 
        35              

