                         SEQUENCE LISTING

<110>  DSM Intellectual Property B.V.
 
<120>  Self-Guiding Integration Construct (SGIC)

<130>  32466-WO-PCT

<150>  EP17165201.9
<151>  2017-04-06

<160>  111   

<170>  PatentIn version 3.5

<210>  1
<211>  5441
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAS9, including a C-terminal SV40 nuclear localization signal, 
       codon pair optimized for expression in S. cerevisiae; the 
       sequence includes the Kl11 promoter from K. lactis and GND2 
       terminator sequence from S. cerevisiae

<400>  1
ttttcttttt ttgcggtcac ccccatgtgg cggggaggca gaggagtagg tagagcaacg       60

aatcctacta tttatccaaa ttagtctagg aactcttttt ctagattttt tagatttgag      120

ggcaagcgct gttaacgact cagaaatgta agcactacgg agtagaacga gaaatccgcc      180

ataggtggaa atcctagcaa aatcttgctt accctagcta gcctcaggta agctagcctt      240

agcctgtcaa atttttttca aaatttggta agtttctact agcaaagcaa acacggttca      300

acaaaccgaa aactccactc attatacgtg gaaaccgaaa caaaaaaaca aaaaccaaaa      360

tactcgccaa tgagaaagtt gctgcgtttc tactttcgag gaagaggaac tgagaggatt      420

gactacgaaa ggggcaaaaa cgagtcgtat tctcccatta ttgtctgcta ccacgcggtc      480

tagtagaata agcaaccagt caacgctaag acaggtaatc aaaataccag tctgctggct      540

acgggctagt ttttacctct tttagaaccc actgtaaaag tccgttgtaa agcccgttct      600

cactgttggc gttttttttt ttttggttta gtttcttatt tttcattttt ttctttcatg      660

accaaaaaca aacaaatctc gcgatttgta ctgcggccac tggggcgtgg ccaaaaaaat      720

gacaaattta gaaaccttag tttctgattt ttcctgttat gaggagatat gataaaaaat      780

attactgctt tattgttttt tttttatcta ctgaaataga gaaacttacc caaggaggag      840

gcaaaaaaaa gagtatatat acagcagcta ccattcagat tttaatatat tcttttctct      900

tcttctacac tattattata ataattttac tatattcatt tttagcttaa aacctcatag      960

aatattattc ttcagtcact cgcttaaata cttatcaaaa atggacaaga aatactctat     1020

tggtttggat atcgggacca actccgtcgg ttgggctgtc atcaccgacg aatacaaggt     1080

tccatccaag aaattcaagg tcttgggtaa cactgacaga cactctatca agaagaattt     1140

gatcggtgct ttgttgttcg actccggtga aaccgctgaa gctaccagat tgaagcgtac     1200

cgctcgtcgt agatacacta gacgtaaaaa ccgtatttgt tacttgcaag aaatcttttc     1260

taacgaaatg gccaaggttg acgactcttt cttccacaga ttggaagaat ctttcttggt     1320

tgaagaagac aagaagcacg aaagacatcc aatcttcggt aacatcgttg acgaagttgc     1380

ttaccacgaa aaatacccta ccatctacca tttgagaaag aagttggtcg attccaccga     1440

caaggctgat ttgagattga tctatttggc cttggctcac atgatcaagt tcagaggtca     1500

cttcttgatt gaaggtgact tgaacccaga caactctgac gtcgacaaat tgttcatcca     1560

attggtccaa acctacaacc aattattcga ggaaaaccca attaacgctt ctggtgttga     1620

tgctaaggcc atcttatctg cccgtttgtc caagtctaga cgtttggaaa acttgattgc     1680

tcaattgcct ggtgaaaaga aaaacggttt gttcggtaac ttgatcgctt tgtccttggg     1740

tttgacccca aacttcaagt ccaacttcga cttggctgaa gatgccaagt tgcaattgtc     1800

caaggacacc tacgacgacg acttagacaa cttgttggct caaatcggtg accaatacgc     1860

cgacttgttc ttggctgcca aaaacttatc tgacgctatc ttgttgtctg acatcttgag     1920

agttaacact gaaattacca aggctccatt gtctgcttct atgatcaaaa gatacgacga     1980

acaccaccaa gatctgactt tgttgaaggc tttggttaga caacaattgc cagaaaagta     2040

caaggaaatc ttcttcgacc aatccaaaaa tggttacgcc ggttacattg acggtggtgc     2100

ttctcaggaa gaattctaca agttcatcaa gccaattttg gaaaagatgg atggtactga     2160

agaattattg gttaagttga acagagaaga cttattgaga aagcaacgta ccttcgataa     2220

cggttctatc ccacaccaaa tccacttggg tgaattgcac gccattttga gaagacagga     2280

agatttctat ccattcctaa aggacaacag agaaaagatc gaaaagatct taactttcag     2340

aatcccatac tacgtcggtc cattggccag aggtaattct agattcgctt ggatgaccag     2400

aaagtctgaa gaaaccatca ccccatggaa cttcgaagaa gtcgtcgaca agggtgcttc     2460

tgcccaatct ttcatcgaaa gaatgaccaa ctttgataag aacttgccaa acgagaaggt     2520

cttgccaaag cactctttgt tgtacgaata cttcaccgtc tacaacgaat taaccaaggt     2580

taaatacgtt actgaaggta tgagaaagcc agctttccta tccggtgaac aaaagaaggc     2640

tattgttgac ttgttgttta agaccaacag aaaggtcact gttaagcaat tgaaggaaga     2700

ctacttcaag aagattgaat gtttcgattc cgtcgaaatc tccggtgttg aagaccgttt     2760

caatgcttct ttgggcacct accacgattt gttaaagatc atcaaggaca aggacttttt     2820

agataacgaa gaaaacgaag acatcttgga agatatcgtt ttgaccttga ctcttttcga     2880

ggacagagaa atgattgaag agagattgaa gacctacgct cacttgttcg acgataaagt     2940

tatgaagcaa ctaaagagaa gaagatacac tggttggggt agattgtcca gaaagttgat     3000

taacggtatc agagacaagc aatccggtaa gactatttta gactttttga aatccgatgg     3060

tttcgctaac agaaacttta tgcaattgat tcacgacgat tctttgactt tcaaggaaga     3120

cattcaaaaa gcccaagtct ctggtcaagg tgattctttg cacgaacaca tcgctaactt     3180

ggctggttct ccagctatta agaagggtat cttacaaacc gtcaaggtcg ttgatgaatt     3240

ggtcaaagtc atgggtagac acaagccaga aaatattgtc atcgaaatgg ctagagaaaa     3300

ccaaactact caaaagggtc aaaagaactc tagagaacgt atgaagagaa ttgaagaagg     3360

tatcaaggag ttgggttctc aaattttgaa agaacaccca gtcgaaaaca ctcaattaca     3420

aaacgaaaag ctatacttgt actacttgca aaacggtcgt gacatgtacg tcgaccaaga     3480

attggatatc aacagattgt ctgactacga tgtcgatcat atcgtcccac aatcgttctt     3540

gaaggacgat tccattgaca acaaagtttt gactagatct gacaagaaca gaggtaagtc     3600

tgataacgtt ccatctgaag aagttgttaa gaagatgaag aactactgga gacaattgtt     3660

gaatgctaag ttgatcactc aaagaaagtt cgacaacttg accaaggctg aaagaggtgg     3720

tttgtccgaa ttggacaaag ccggtttcat caagagacaa ttagtcgaaa ctagacaaat     3780

caccaagcat gttgctcaaa tcttggattc cagaatgaac actaagtacg atgaaaacga     3840

caaactaatt agagaagtta aggtcatcac tttgaagtct aagttggttt ctgacttcag     3900

aaaggacttc caattttaca aggtcagaga aatcaacaac taccatcacg ctcacgatgc     3960

ctacttgaac gctgttgtcg gtactgcctt aatcaaaaag tacccaaagt tggaatctga     4020

attcgtttac ggtgactaca aggtttacga tgttagaaag atgatcgcca agtctgaaca     4080

agaaattggt aaggccactg ctaagtactt cttctactct aacatcatga actttttcaa     4140

gactgaaatc actttagcta acggtgaaat tagaaagcgt ccattgattg aaaccaatgg     4200

tgaaactggt gaaattgtct gggacaaggg tagagatttc gctaccgtca gaaaggtttt     4260

gtctatgcca caagttaaca tcgtcaagaa gactgaagtt caaactggtg gtttctctaa     4320

ggaatccatt ttgccaaaga gaaactctga caagttgatt gctagaaaga aggactggga     4380

tcctaagaag tacggtggtt tcgactctcc aactgttgct tactccgttt tggtcgttgc     4440

taaggttgaa aagggtaagt ctaagaagtt gaagtctgtt aaggaattgt tgggtatcac     4500

catcatggaa agatcctcct tcgaaaagaa cccaatcgac tttttggaag ctaagggtta     4560

caaggaagtc aagaaggatt tgatcattaa gttaccaaaa tactccttgt tcgaattgga     4620

aaacggtaga aagagaatgt tggcctccgc tggtgaacta caaaaaggta acgaattggc     4680

tttaccatct aagtacgtta acttcttgta cttggcttcc cactacgaaa agttgaaagg     4740

ttccccagaa gacaacgaac aaaagcaatt gtttgttgaa caacacaagc actacttgga     4800

tgaaattatt gaacaaatct ccgaattctc caagagagtc attttggctg atgctaactt     4860

agataaggtt ttatccgctt acaacaagca cagagacaaa ccaatcagag aacaagctga     4920

aaacatcatt catttgttca ctttaaccaa cttgggtgct ccagctgctt tcaaatactt     4980

cgacactacc attgacagaa agagatacac ttccaccaaa gaagttttag atgctacttt     5040

gattcaccaa tctattaccg gtttgtacga aaccagaatt gacttgtctc aattgggtgg     5100

tgattccaga gctgatccaa agaagaagag aaaggtgtaa aggagttaaa ggcaaagttt     5160

tcttttctag agccgttccc acaaataatt atacgtatat gcttcttttc gtttactata     5220

tatctatatt tacaagcctt tattcactga tgcaatttgt ttccaaatac ttttttggag     5280

atctcataac tagatatcat gatggcgcaa cttggcgcta tcttaattac tctggctgcc     5340

aggcccgtgt agagggccgc aagaccttct gtacgccata tagtctctaa gaacttgaac     5400

aagtttctag acctattgcc gcctttcgga tcgctattgt t                         5441


<210>  2
<211>  11742
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid vector pCSN061

<400>  2
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat      240

atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa      300

aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa      360

gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat      420

tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta      480

atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg      540

cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa      600

agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa      660

atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg      720

ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt      780

ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag      840

actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg      900

attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg      960

gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa     1020

atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg     1080

agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat     1140

aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga     1200

aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt     1260

ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa     1320

atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca     1380

gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc     1440

gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg     1500

aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg     1560

ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg     1620

gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg     1680

ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga     1740

tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga     1800

ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag     1860

cgcgcgtaat acgactcact atagggcgaa ttgggtacct tttctttttt tgcggtcacc     1920

cccatgtggc ggggaggcag aggagtaggt agagcaacga atcctactat ttatccaaat     1980

tagtctagga actctttttc tagatttttt agatttgagg gcaagcgctg ttaacgactc     2040

agaaatgtaa gcactacgga gtagaacgag aaatccgcca taggtggaaa tcctagcaaa     2100

atcttgctta ccctagctag cctcaggtaa gctagcctta gcctgtcaaa tttttttcaa     2160

aatttggtaa gtttctacta gcaaagcaaa cacggttcaa caaaccgaaa actccactca     2220

ttatacgtgg aaaccgaaac aaaaaaacaa aaaccaaaat actcgccaat gagaaagttg     2280

ctgcgtttct actttcgagg aagaggaact gagaggattg actacgaaag gggcaaaaac     2340

gagtcgtatt ctcccattat tgtctgctac cacgcggtct agtagaataa gcaaccagtc     2400

aacgctaaga caggtaatca aaataccagt ctgctggcta cgggctagtt tttacctctt     2460

ttagaaccca ctgtaaaagt ccgttgtaaa gcccgttctc actgttggcg tttttttttt     2520

tttggtttag tttcttattt ttcatttttt tctttcatga ccaaaaacaa acaaatctcg     2580

cgatttgtac tgcggccact ggggcgtggc caaaaaaatg acaaatttag aaaccttagt     2640

ttctgatttt tcctgttatg aggagatatg ataaaaaata ttactgcttt attgtttttt     2700

ttttatctac tgaaatagag aaacttaccc aaggaggagg caaaaaaaag agtatatata     2760

cagcagctac cattcagatt ttaatatatt cttttctctt cttctacact attattataa     2820

taattttact atattcattt ttagcttaaa acctcataga atattattct tcagtcactc     2880

gcttaaatac ttatcaaaaa tggacaagaa atactctatt ggtttggata tcgggaccaa     2940

ctccgtcggt tgggctgtca tcaccgacga atacaaggtt ccatccaaga aattcaaggt     3000

cttgggtaac actgacagac actctatcaa gaagaatttg atcggtgctt tgttgttcga     3060

ctccggtgaa accgctgaag ctaccagatt gaagcgtacc gctcgtcgta gatacactag     3120

acgtaaaaac cgtatttgtt acttgcaaga aatcttttct aacgaaatgg ccaaggttga     3180

cgactctttc ttccacagat tggaagaatc tttcttggtt gaagaagaca agaagcacga     3240

aagacatcca atcttcggta acatcgttga cgaagttgct taccacgaaa aataccctac     3300

catctaccat ttgagaaaga agttggtcga ttccaccgac aaggctgatt tgagattgat     3360

ctatttggcc ttggctcaca tgatcaagtt cagaggtcac ttcttgattg aaggtgactt     3420

gaacccagac aactctgacg tcgacaaatt gttcatccaa ttggtccaaa cctacaacca     3480

attattcgag gaaaacccaa ttaacgcttc tggtgttgat gctaaggcca tcttatctgc     3540

ccgtttgtcc aagtctagac gtttggaaaa cttgattgct caattgcctg gtgaaaagaa     3600

aaacggtttg ttcggtaact tgatcgcttt gtccttgggt ttgaccccaa acttcaagtc     3660

caacttcgac ttggctgaag atgccaagtt gcaattgtcc aaggacacct acgacgacga     3720

cttagacaac ttgttggctc aaatcggtga ccaatacgcc gacttgttct tggctgccaa     3780

aaacttatct gacgctatct tgttgtctga catcttgaga gttaacactg aaattaccaa     3840

ggctccattg tctgcttcta tgatcaaaag atacgacgaa caccaccaag atctgacttt     3900

gttgaaggct ttggttagac aacaattgcc agaaaagtac aaggaaatct tcttcgacca     3960

atccaaaaat ggttacgccg gttacattga cggtggtgct tctcaggaag aattctacaa     4020

gttcatcaag ccaattttgg aaaagatgga tggtactgaa gaattattgg ttaagttgaa     4080

cagagaagac ttattgagaa agcaacgtac cttcgataac ggttctatcc cacaccaaat     4140

ccacttgggt gaattgcacg ccattttgag aagacaggaa gatttctatc cattcctaaa     4200

ggacaacaga gaaaagatcg aaaagatctt aactttcaga atcccatact acgtcggtcc     4260

attggccaga ggtaattcta gattcgcttg gatgaccaga aagtctgaag aaaccatcac     4320

cccatggaac ttcgaagaag tcgtcgacaa gggtgcttct gcccaatctt tcatcgaaag     4380

aatgaccaac tttgataaga acttgccaaa cgagaaggtc ttgccaaagc actctttgtt     4440

gtacgaatac ttcaccgtct acaacgaatt aaccaaggtt aaatacgtta ctgaaggtat     4500

gagaaagcca gctttcctat ccggtgaaca aaagaaggct attgttgact tgttgtttaa     4560

gaccaacaga aaggtcactg ttaagcaatt gaaggaagac tacttcaaga agattgaatg     4620

tttcgattcc gtcgaaatct ccggtgttga agaccgtttc aatgcttctt tgggcaccta     4680

ccacgatttg ttaaagatca tcaaggacaa ggacttttta gataacgaag aaaacgaaga     4740

catcttggaa gatatcgttt tgaccttgac tcttttcgag gacagagaaa tgattgaaga     4800

gagattgaag acctacgctc acttgttcga cgataaagtt atgaagcaac taaagagaag     4860

aagatacact ggttggggta gattgtccag aaagttgatt aacggtatca gagacaagca     4920

atccggtaag actattttag actttttgaa atccgatggt ttcgctaaca gaaactttat     4980

gcaattgatt cacgacgatt ctttgacttt caaggaagac attcaaaaag cccaagtctc     5040

tggtcaaggt gattctttgc acgaacacat cgctaacttg gctggttctc cagctattaa     5100

gaagggtatc ttacaaaccg tcaaggtcgt tgatgaattg gtcaaagtca tgggtagaca     5160

caagccagaa aatattgtca tcgaaatggc tagagaaaac caaactactc aaaagggtca     5220

aaagaactct agagaacgta tgaagagaat tgaagaaggt atcaaggagt tgggttctca     5280

aattttgaaa gaacacccag tcgaaaacac tcaattacaa aacgaaaagc tatacttgta     5340

ctacttgcaa aacggtcgtg acatgtacgt cgaccaagaa ttggatatca acagattgtc     5400

tgactacgat gtcgatcata tcgtcccaca atcgttcttg aaggacgatt ccattgacaa     5460

caaagttttg actagatctg acaagaacag aggtaagtct gataacgttc catctgaaga     5520

agttgttaag aagatgaaga actactggag acaattgttg aatgctaagt tgatcactca     5580

aagaaagttc gacaacttga ccaaggctga aagaggtggt ttgtccgaat tggacaaagc     5640

cggtttcatc aagagacaat tagtcgaaac tagacaaatc accaagcatg ttgctcaaat     5700

cttggattcc agaatgaaca ctaagtacga tgaaaacgac aaactaatta gagaagttaa     5760

ggtcatcact ttgaagtcta agttggtttc tgacttcaga aaggacttcc aattttacaa     5820

ggtcagagaa atcaacaact accatcacgc tcacgatgcc tacttgaacg ctgttgtcgg     5880

tactgcctta atcaaaaagt acccaaagtt ggaatctgaa ttcgtttacg gtgactacaa     5940

ggtttacgat gttagaaaga tgatcgccaa gtctgaacaa gaaattggta aggccactgc     6000

taagtacttc ttctactcta acatcatgaa ctttttcaag actgaaatca ctttagctaa     6060

cggtgaaatt agaaagcgtc cattgattga aaccaatggt gaaactggtg aaattgtctg     6120

ggacaagggt agagatttcg ctaccgtcag aaaggttttg tctatgccac aagttaacat     6180

cgtcaagaag actgaagttc aaactggtgg tttctctaag gaatccattt tgccaaagag     6240

aaactctgac aagttgattg ctagaaagaa ggactgggat cctaagaagt acggtggttt     6300

cgactctcca actgttgctt actccgtttt ggtcgttgct aaggttgaaa agggtaagtc     6360

taagaagttg aagtctgtta aggaattgtt gggtatcacc atcatggaaa gatcctcctt     6420

cgaaaagaac ccaatcgact ttttggaagc taagggttac aaggaagtca agaaggattt     6480

gatcattaag ttaccaaaat actccttgtt cgaattggaa aacggtagaa agagaatgtt     6540

ggcctccgct ggtgaactac aaaaaggtaa cgaattggct ttaccatcta agtacgttaa     6600

cttcttgtac ttggcttccc actacgaaaa gttgaaaggt tccccagaag acaacgaaca     6660

aaagcaattg tttgttgaac aacacaagca ctacttggat gaaattattg aacaaatctc     6720

cgaattctcc aagagagtca ttttggctga tgctaactta gataaggttt tatccgctta     6780

caacaagcac agagacaaac caatcagaga acaagctgaa aacatcattc atttgttcac     6840

tttaaccaac ttgggtgctc cagctgcttt caaatacttc gacactacca ttgacagaaa     6900

gagatacact tccaccaaag aagttttaga tgctactttg attcaccaat ctattaccgg     6960

tttgtacgaa accagaattg acttgtctca attgggtggt gattccagag ctgatccaaa     7020

gaagaagaga aaggtgtaaa ggagttaaag gcaaagtttt cttttctaga gccgttccca     7080

caaataatta tacgtatatg cttcttttcg tttactatat atctatattt acaagccttt     7140

attcactgat gcaatttgtt tccaaatact tttttggaga tctcataact agatatcatg     7200

atggcgcaac ttggcgctat cttaattact ctggctgcca ggcccgtgta gagggccgca     7260

agaccttctg tacgccatat agtctctaag aacttgaaca agtttctaga cctattgccg     7320

cctttcggat cgctattgtt gcggccgcca gctgaagctt cgtacgctgc aggtcgacga     7380

attctaccgt tcgtataatg tatgctatac gaagttatag atctgtttag cttgcctcgt     7440

ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt gacagtcttg     7500

acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc     7560

atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg     7620

gctcctcgct gcagacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc     7680

cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct     7740

ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata     7800

aacaaccatg ggtaaggaaa agactcacgt ttcgaggccg cgattaaatt ccaacatgga     7860

tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat     7920

ctatcgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag     7980

cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc     8040

tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc     8100

gatccccggc aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat     8160

tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc     8220

ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt     8280

ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa     8340

agaaatgcat aagcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc     8400

acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt     8460

cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc     8520

tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa     8580

attgcagttt catttgatgc tcgatgagtt tttctaatca gtactgacaa taaaaagatt     8640

cttgttttca agaacttgtc atttgtatag tttttttata ttgtagttgt tctattttaa     8700

tcaaatgtta gcgtgattta tatttttttt cgcctcgaca tcatctgccc agatgcgaag     8760

ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt cgctatactg     8820

ctgtcgattc gatactaacg ccgccatcca gtgtcgaaaa cgagctcata acttcgtata     8880

atgtatgcta tacgaacggt agaattcgaa tcagatccac tagtggccta tgcggccgcc     8940

accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta     9000

atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat     9060

aggagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgaggt aactcacatt     9120

aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta     9180

atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc     9240

gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa     9300

ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa     9360

aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct     9420

ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac     9480

aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc     9540

gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc     9600

tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg     9660

tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga     9720

gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag     9780

cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta     9840

cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag     9900

agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg     9960

caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac    10020

ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc    10080

aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag    10140

tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc    10200

agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac    10260

gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc    10320

accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg    10380

tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag    10440

tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc    10500

acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac    10560

atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag    10620

aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac    10680

tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg    10740

agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc    10800

gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact    10860

ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg    10920

atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa    10980

tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt    11040

tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg    11100

tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg    11160

gtccttttca tcacgtgcta taaaaataat tataatttaa attttttaat ataaatatat    11220

aaattaaaaa tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg ttttccgaag    11280

atgtaaaaga ctctaggggg atcgccaaca aatactacct tttatcttgc tcttcctgct    11340

ctcaggtatt aatgccgaat tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc    11400

ctgtgatttt acattttact tatcgttaat cgaatgtata tctatttaat ctgcttttct    11460

tgtctaataa atatatatgt aaagtacgct ttttgttgaa attttttaaa cctttgttta    11520

tttttttttc ttcattccgt aactcttcta ccttctttat ttactttcta aaatccaaat    11580

acaaaacata aaaataaata aacacagagt aaattcccaa attattccat cattaaaaga    11640

tacgaggcgc gtgtaagtta caggcaagcg atccgtccta agaaaccatt attatcatga    11700

cattaaccta taaaaatagg cgtatcacga ggccctttcg tc                       11742


<210>  3
<211>  5712
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid vector pRN1120

<400>  3
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc      240

accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca      300

ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat      360

taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc      420

ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc      480

aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt      540

agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg      600

tttctattat gaatttcatt tataaagttt atgtacacct aggatccgtc gacactggat      660

ggcggcgtta gtatcgaatc gacagcagta tagcgaccag cattcacata cgattgacgc      720

atgatattac tttctgcgca cttaacttcg catctgggca gatgatgtcg aggcgaaaaa      780

aaatataaat cacgctaaca tttgattaaa atagaacaac tacaatataa aaaaactata      840

caaatgacaa gttcttgaaa acaagaatct ttttattgtc agtactaggg gcagggcatg      900

ctcatgtaga gcgcctgctc gccgtccgag gcggtgccgt cgtacagggc ggtgtccagg      960

ccgcagaggg tgaaccccat ccgccggtac gcgtggatcg ccggtgcgtt gacgttggtg     1020

acctccagcc agaggtgccc ggcgccccgc tcgcgggcga actccgtcgc gagccccatc     1080

aacgcgcgcc cgaccccgtg cccccggtgc tccggggcga cctcgatgtc ctcgacggtc     1140

agccggcggt tccagccgga gtacgagacg accacgaagc ccgccaggtc gccgtcgtcc     1200

ccgtacgcga cgaacgtccg ggagtccggg tcgccgtcct ccccggcgtc cgattcgtcg     1260

tccgattcgt cgtcggggaa caccttggtc aggggcgggt ccaccggcac ctcccgcagg     1320

gtgaagccgt ccccggtggc ggtgacgcgg aagacggtgt cggtggtgaa ggacccatcc     1380

agtgcctcga tggcctcggc gtcccccggg acactggtgc ggtaccggta agccgtgtcg     1440

tcaagagtgg tcattttaca tggttgttta tgttcggatg tgatgtgaga actgtatcct     1500

agcaagattt taaaaggaag tatatgaaag aagaacctca gtggcaaatc ctaacctttt     1560

atatttctct acaggggcgc ggcgtgggga caattcaacg cgtctgtgag gggagcgttt     1620

ccctgctcgc aggtctgcag cgaggagccg taatttttgc ttcgcgccgt gcggccatca     1680

aaatgtatgg atgcaaatga ttatacatgg ggatgtatgg gctaaatgta cgggcgacag     1740

tcacatcatg cccctgagct gcgcacgtca agactgtcaa ggagggtatt ctgggcctcc     1800

atgtcgctgg ccgggtgacc cggcggggac gaggccttaa gttcgaacgt acgagctccg     1860

gcattgcgaa taccgctttc cacaaacatt gctcaaaagt atctctttgc tatatatctc     1920

tgtgctatat ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa     1980

ctcgacctct acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta     2040

gtaagaacta ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg     2100

tagtatatag agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat     2160

caacgctatc actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc     2220

ggggatgcct ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg     2280

gaagtggagt caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta     2340

accttaacgg acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag     2400

gagaaaaaaa gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt     2460

gtagaacaaa aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt     2520

ctgttctgta aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca     2580

tttttgtttt acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg     2640

catttctgtt ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg     2700

ttgcattttt gttctacaaa atgaagcaca gatgcttcgt taacaaagat atgctattga     2760

agtgcaagat ggaaacgcag aaaatgaacc ggggatgcga cgtgcaagat tacctatgca     2820

atagatgcaa tagtttctcc aggaaccgaa atacatacat tgtcttccgt aaagcgctag     2880

actatatatt attatacagg ttcaaatata ctatctgttt cagggaaaac tcccaggttc     2940

ggatgttcaa aattcaatga tgggtaacaa gtacgatcgt aaatctgtaa aacagtttgt     3000

cggatattag gctgtatctc ctcaaagcgt attcgaatat cattgagaag ctgcagcgtc     3060

acatcggata ataatgatgg cagccattgt agaagtgcct tttgcatttc tagtctcttt     3120

ctcggtctag ctagttttac tacatcgcga agatagaatc ttagatcaca ctgcctttgc     3180

tgagctggat caatagagta acaaaagagt ggtaaggcct cgttaaagga caaggacctg     3240

agcggaagtg tatcgtacag tagacggagt atactaggta tagtctatag tccgtggaat     3300

taattctcat gtttgacagc ttatcatcga taatccggag ctagcatgcg gccgctctag     3360

aactagtgga tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct     3420

cgaggggggg cccggtaccc agcttttgtt ccctttagtg agggttaatt ccgagcttgg     3480

cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca     3540

acataggagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg aggtaactca     3600

cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc     3660

attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt     3720

cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact     3780

caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag     3840

caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata     3900

ggctcggccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc     3960

cgacaggact ataaagatac caggcgttcc cccctggaag ctccctcgtg cgctctcctg     4020

ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc     4080

tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg     4140

gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc     4200

ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga     4260

ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg     4320

gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa     4380

aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg     4440

tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt     4500

ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat     4560

tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct     4620

aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta     4680

tctcagcgat ctgtctattt cgttcatcca tagttgcctg actgcccgtc gtgtagataa     4740

ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac     4800

gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa     4860

gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag     4920

taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg     4980

tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag     5040

ttacatgatc ccccatgttg tgaaaaaaag cggttagctc cttcggtcct ccgatcgttg     5100

tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc     5160

ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat     5220

tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata     5280

ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa     5340

aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca     5400

actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc     5460

aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc     5520

tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg     5580

aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac     5640

ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga     5700

ggccctttcg tc                                                         5712


<210>  4
<211>  588
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gBlock of the guide RNA expression cassette to target CAS9 to the
       INT1 locus

<400>  4
tatagtccgt ggaattaatt ctcatgtttg acagcttatc atcgataatc cggagctagc       60

atgcggccgc tctagaacta gtggatcccc cgggctgcag tctttgaaaa gataatgtat      120

gattatgctt tcactcatat ttatacagaa acttgatgtt ttctttcgag tatatacaag      180

gtgattacat gtacgtttga agtacaactc tagattttgt agtgccctct tgggctagcg      240

gtaaaggtgc gcattttttc acaccctaca atgttctgtt caaaagattt tggtcaaacg      300

ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga aacttctccg cagtgaaaga      360

taaatgatct attagaacca gggaggtccg ttttagagct agaaatagca agttaaaata      420

aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtggtgctt tttttgtttt      480

ttatgtctgg ggggcccggt acccagcttt tgttcccttt agtgagggtt aattccgagc      540

ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccg                   588


<210>  5
<211>  588
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gBlock of the guide RNA expression cassette to target CAS9 to the
       INT59 locus

<400>  5
tatagtccgt ggaattaatt ctcatgtttg acagcttatc atcgataatc cggagctagc       60

atgcggccgc tctagaacta gtggatcccc cgggctgcag tctttgaaaa gataatgtat      120

gattatgctt tcactcatat ttatacagaa acttgatgtt ttctttcgag tatatacaag      180

gtgattacat gtacgtttga agtacaactc tagattttgt agtgccctct tgggctagcg      240

gtaaaggtgc gcattttttc acaccctaca atgttctgtt caaaagattt tggtcaaacg      300

ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga aacttctccg cagtgaaaga      360

taaatgatca gaaaactctt agcttttccg ttttagagct agaaatagca agttaaaata      420

aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtggtgctt tttttgtttt      480

ttatgtctgg ggggcccggt acccagcttt tgttcccttt agtgagggtt aattccgagc      540

ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccg                   588


<210>  6
<211>  588
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gBlock of the guide RNA expression cassette to target CAS9 to the
       YPRCtau3 locus

<400>  6
tatagtccgt ggaattaatt ctcatgtttg acagcttatc atcgataatc cggagctagc       60

atgcggccgc tctagaacta gtggatcccc cgggctgcag tctttgaaaa gataatgtat      120

gattatgctt tcactcatat ttatacagaa acttgatgtt ttctttcgag tatatacaag      180

gtgattacat gtacgtttga agtacaactc tagattttgt agtgccctct tgggctagcg      240

gtaaaggtgc gcattttttc acaccctaca atgttctgtt caaaagattt tggtcaaacg      300

ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga aacttctccg cagtgaaaga      360

taaatgatca gaaaactctt agcttttccg ttttagagct agaaatagca agttaaaata      420

aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtggtgctt tttttgtttt      480

ttatgtctgg ggggcccggt acccagcttt tgttcccttt agtgagggtt aattccgagc      540

ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccg                   588


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guide sequence of the INT1 integration site

<400>  7
tattagaacc agggaggtcc                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guide sequence of the INT59 integration site

<400>  8
agaaaactct tagcttttcc                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guide sequence of the YPRCtau3 integration site

<400>  9
caatatggta tgccgagtct                                                   20


<210>  10
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FW PCR primer to obtain INT1 SGIC donor DNA sequence for 
       integration, 0 bp deletion

<400>  10
acttctctac attctctgac tttttaaaac tgtgtactgg cgaccaatcg tctttgaaaa       60

gataatgtat gattatg                                                      77


<210>  11
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  REV PCR primer to obtain INT1 SGIC donor DNA sequence for 
       integration, 0 bp deletion

<400>  11
ggaaattttc aaaggcgttg gatcaaaaaa taggccttta tttcatcgcg agacataaaa       60

aacaaaaaaa gcacc                                                        75


<210>  12
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FW PCR primer to obtain INT1 SGIC donor DNA sequence for 
       integration, 1 kbp deletion

<400>  12
cttcatgcca gcaatagttg cgtgctgagc tcaacagtgc ccaacccttg tctttgaaaa       60

gataatgtat gattatg                                                      77


<210>  13
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  REV PCR primer to obtain INT1 SGIC donor DNA sequence for 
       integration, 1 kbp deletion

<400>  13
gaaaagcact cctttagtac cactcaacaa gttgtctgat gacaaagaat agacataaaa       60

aacaaaaaaa gcacc                                                        75


<210>  14
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FW PCRprimer to obtain INT59 SGIC donor DNA sequence for 
       integration, 0 bp deletion

<400>  14
cgccaagagc ccaagaaaga gacacaaaac tacgtgggat aagcttgggg tctttgaaaa       60

gataatgtat gattatg                                                      77


<210>  15
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  REV PCR primer to obtain INT59 SGIC donor DNA sequence for 
       integration, 0 bp deletion

<400>  15
tattgcggga ttttttggtg ccgtacgccg gagccgacgg aggtaagtca agacataaaa       60

aacaaaaaaa gcacc                                                        75


<210>  16
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FW PCR primer to obtain INT59 SGIC donor DNA sequence for 
       integration, 1 kbp deletion

<400>  16
acgaggtgaa gggcaaaggt gaattaacca aagtgaagag gacgacgtag tctttgaaaa       60

gataatgtat gattatg                                                      77


<210>  17
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  REV PCR primer to obtain INT59 SGIC donor DNA sequence for 
       integration, 1 kbp deletion

<400>  17
tgatatgagt tgtggtgatt ggagagagta aaagaaagaa tgatagatgc agacataaaa       60

aacaaaaaaa gcacc                                                        75


<210>  18
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FW PCR primer to obtain YPRCtau3 SGIC donor DNA sequence for 
       integration, 0 bp deletion

<400>  18
catcttttaa tgcctactct tttgatgttc acttacatgt tacaatgaag tctttgaaaa       60

gataatgtat gattatg                                                      77


<210>  19
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  REV PCR primer to obtain YPRCtau3 SGIC donor DNA sequence for 
       integration, 0 bp deletion

<400>  19
gcccctctta tacgattata ttaagatcca tattcaacct tcattaatac agacataaaa       60

aacaaaaaaa gcacc                                                        75


<210>  20
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FW PCR primer to obtain YPRCtau3 SGIC donor DNA sequence for 
       integration, 1 kbp deletion

<400>  20
tcatacgatt tccacatgtg tctcatatat attttatgtt taggttaata tctttgaaaa       60

gataatgtat gattatg                                                      77


<210>  21
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  REV PCR primer to obtain YPRCtau3 SGIC donor DNA sequence for 
       integration, 1 kbp deletion

<400>  21
cttatgtatt tttaatcgtc cttgtatgga agtatcaaag gggacgttct agacataaaa       60

aacaaaaaaa gcacc                                                        75


<210>  22
<211>  488
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  INT1 SGIC sequence for integration, 0 bp deletion

<400>  22
acttctctac attctctgac tttttaaaac tgtgtactgg cgaccaatcg tctttgaaaa       60

gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt ttctttcgag      120

tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt agtgccctct      180

tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt caaaagattt      240

tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga aacttctccg      300

cagtgaaaga taaatgatct attagaacca gggaggtccg ttttagagct agaaatagca      360

agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtggtgctt      420

tttttgtttt ttatgtctcg cgatgaaata aaggcctatt ttttgatcca acgcctttga      480

aaatttcc                                                               488


<210>  23
<211>  488
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  INT1 SGIC sequence for integration, 1 kb deletion

<400>  23
cttcatgcca gcaatagttg cgtgctgagc tcaacagtgc ccaacccttg tctttgaaaa       60

gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt ttctttcgag      120

tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt agtgccctct      180

tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt caaaagattt      240

tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga aacttctccg      300

cagtgaaaga taaatgatct attagaacca gggaggtccg ttttagagct agaaatagca      360

agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtggtgctt      420

tttttgtttt ttatgtctat tctttgtcat cagacaactt gttgagtggt actaaaggag      480

tgcttttc                                                               488


<210>  24
<211>  488
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  INT59 SGIC sequence for integration, 0 bp deletion

<400>  24
cgccaagagc ccaagaaaga gacacaaaac tacgtgggat aagcttgggg tctttgaaaa       60

gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt ttctttcgag      120

tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt agtgccctct      180

tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt caaaagattt      240

tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga aacttctccg      300

cagtgaaaga taaatgatca gaaaactctt agcttttccg ttttagagct agaaatagca      360

agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtggtgctt      420

tttttgtttt ttatgtcttg acttacctcc gtcggctccg gcgtacggca ccaaaaaatc      480

ccgcaata                                                               488


<210>  25
<211>  488
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  INT59 SGIC sequence for integration, 1 kb deletion

<400>  25
acgaggtgaa gggcaaaggt gaattaacca aagtgaagag gacgacgtag tctttgaaaa       60

gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt ttctttcgag      120

tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt agtgccctct      180

tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt caaaagattt      240

tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga aacttctccg      300

cagtgaaaga taaatgatca gaaaactctt agcttttccg ttttagagct agaaatagca      360

agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtggtgctt      420

tttttgtttt ttatgtctgc atctatcatt ctttctttta ctctctccaa tcaccacaac      480

tcatatca                                                               488


<210>  26
<211>  488
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YPRCtau3 SGIC sequence for integration, 0 bp deletion

<400>  26
catcttttaa tgcctactct tttgatgttc acttacatgt tacaatgaag tctttgaaaa       60

gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt ttctttcgag      120

tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt agtgccctct      180

tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt caaaagattt      240

tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga aacttctccg      300

cagtgaaaga taaatgatcc aatatggtat gccgagtctg ttttagagct agaaatagca      360

agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtggtgctt      420

tttttgtttt ttatgtctgt attaatgaag gttgaatatg gatcttaata taatcgtata      480

agaggggc                                                               488


<210>  27
<211>  488
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YPRCtau3 SGIC sequence for integration, 1 kb deletion

<400>  27
tcatacgatt tccacatgtg tctcatatat attttatgtt taggttaata tctttgaaaa       60

gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt ttctttcgag      120

tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt agtgccctct      180

tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt caaaagattt      240

tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga aacttctccg      300

cagtgaaaga taaatgatcc aatatggtat gccgagtctg ttttagagct agaaatagca      360

agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtggtgctt      420

tttttgtttt ttatgtctag aacgtcccct ttgatacttc catacaagga cgattaaaaa      480

tacataag                                                               488


<210>  28
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FW PCR primer annealing to SNR52p to obtain SGIC sequence for 
       integration without genomic flanking regions attached

<400>  28
tctttgaaaa gataatgtat gattatg                                           27


<210>  29
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  REV PCR primer annealing to SUP4 3' flanking region to obtain 
       SGIC sequence for integration without genomic flanking regions 
       attached

<400>  29
agacataaaa aacaaaaaaa gcacc                                             25


<210>  30
<211>  388
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  INT1 SGIC without genomic flanking regions attached on either 
       side

<400>  30
tctttgaaaa gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt       60

ttctttcgag tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt      120

agtgccctct tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt      180

caaaagattt tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga      240

aacttctccg cagtgaaaga taaatgatct attagaacca gggaggtccg ttttagagct      300

agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc      360

ggtggtgctt tttttgtttt ttatgtct                                         388


<210>  31
<211>  388
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  INT59 SGIC without genomic flanking regions attached on either 
       side

<400>  31
tctttgaaaa gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt       60

ttctttcgag tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt      120

agtgccctct tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt      180

caaaagattt tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga      240

aacttctccg cagtgaaaga taaatgatca gaaaactctt agcttttccg ttttagagct      300

agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc      360

ggtggtgctt tttttgtttt ttatgtct                                         388


<210>  32
<211>  388
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YPRCtau3 SGIC without genomic flanking regions attached on either
       side

<400>  32
tctttgaaaa gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt       60

ttctttcgag tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt      120

agtgccctct tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt      180

caaaagattt tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga      240

aacttctccg cagtgaaaga taaatgatcc aatatggtat gccgagtctg ttttagagct      300

agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc      360

ggtggtgctt tttttgtttt ttatgtct                                         388


<210>  33
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FW PCR primer to confirm integration of the SGIC in the INT1 
       locus, 0 bp deletion.

<400>  33
ttacatttca ggtttccata atatg                                             25


<210>  34
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  REV PCR primer to confirm integration of the SGIC in the INT1 
       locus, 0 bp deletion

<400>  34
ctttcataag ctaattatgc catc                                              24


<210>  35
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FW PCR primer to confirm integration of the SGIC in the INT1 
       locus, 1 kbp deletion

<400>  35
tgggttcata ctgctgtgtt atag                                              24


<210>  36
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  REV PCR primer to confirm integration of the SGIC in the INT1 
       locus, 1 kbp deletion

<400>  36
aactagctca gaaaacacta acg                                               23


<210>  37
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FW PCR primer to confirm integration of the SGIC in the INT59 
       locus, 0 bp deletion

<400>  37
cagacgtagg aggtagccg                                                    19


<210>  38
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  REV PCR primer to confirm integration of the SGIC in the INT59 
       locus, 0 bp deletion

<400>  38
tcacggttct gcggacgcc                                                    19


<210>  39
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FW PCR primer to confirm integration of the SGIC in the INT59 
       locus, 1 kbp deletion

<400>  39
gaacattaat gactgcaaca cc                                                22


<210>  40
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  REV PCR primer to confirm integration of the SGIC in the INT59 
       locus, 1 kbp deletion

<400>  40
cacaaacgga aaggatatag aag                                               23


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FW PCR primer to confirm integration of the SGIC in the YPRCtau3 
       locus, 0 bp deletion

<400>  41
caagtacagt gctgacgtcc                                                   20


<210>  42
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  REV primer to confirm integration of the SGIC in the YPRCtau3 
       locus, 0 bp deletion

<400>  42
attaatgttg aaccaatcgg cg                                                22


<210>  43
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FW PCR primer to confirm integration of the SGIC in the YPRCtau3 
       locus, 1 kbp deletion

<400>  43
cctctttaca agcggagctt ac                                                22


<210>  44
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  REV PCR primer to confirm integration of the SGIC in the YPRCtau3
       locus, 1 kbp deletion

<400>  44
gcgcttattt atcggcattg ag                                                22


<210>  45
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FW primer annealing to SNR52p to obtain INT1 SGIC DNA sequence 
       with 50 bp connector sequence at the 5' end

<400>  45
aagcgacttc caatcgcttt gcatatccag taccacaccc acaggcgttt tctttgaaaa       60

gataatgtat gattatg                                                      77


<210>  46
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  REV primer annealing to SUP4 to obtain INT1 SGIC DNA sequence 
       with 50 bp connector sequence at the 3' end

<400>  46
acttagtatg gtctgttgga aaggattgtg gcttcgcata caggctttct agacataaaa       60

aacaaaaaaa gcacc                                                        75


<210>  47
<211>  488
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SGIC DNA with connector sequences attached to the 5' and 3' ends

<400>  47
aagcgacttc caatcgcttt gcatatccag taccacaccc acaggcgttt tctttgaaaa       60

gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt ttctttcgag      120

tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt agtgccctct      180

tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt caaaagattt      240

tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga aacttctccg      300

cagtgaaaga taaatgatct attagaacca gggaggtccg ttttagagct agaaatagca      360

agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtggtgctt      420

tttttgtttt ttatgtctag aaagcctgta tgcgaagcca caatcctttc caacagacca      480

tactaagt                                                               488


<210>  48
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  REV primer annealing to SNR52p to obtain the 5' split SGIC DNA 
       sequence targeting INT1

<400>  48
attttaactt gctatttcta gctctaaaac ggacctccct ggttctaata gatcatttat       60

ctttcactgc ggag                                                         74


<210>  49
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FW primer annealing to the guide-RNA to obtain the 3' split SGIC 
       DNA sequence targeting INT1

<400>  49
aaacttctcc gcagtgaaag ataaatgatc tattagaacc agggaggtcc gttttagagc       60

tagaaatagc aag                                                          73


<210>  50
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FW primer annealing to the 5' connector of SGIC DNA fragment to 
       attach genomic DNA sequence for integration on INT1

<400>  50
cttcatgcca gcaatagttg cgtgctgagc tcaacagtgc ccaacccttg aagcgacttc       60

caatcgcttt gc                                                           72


<210>  51
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RV primer annealing to the 3' connector of SGIC DNA fragment to 
       attach genomic DNA sequence for integration on INT1

<400>  51
gaaaagcact cctttagtac cactcaacaa gttgtctgat gacaaagaat acttagtatg       60

gtctgttgga aagg                                                         74


<210>  52
<211>  588
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SGIC DNA with 50 bp genomic DNA sequences attached on both the 5'
       and 3' end for integration on INT1

<400>  52
cttcatgcca gcaatagttg cgtgctgagc tcaacagtgc ccaacccttg aagcgacttc       60

caatcgcttt gcatatccag taccacaccc acaggcgttt tctttgaaaa gataatgtat      120

gattatgctt tcactcatat ttatacagaa acttgatgtt ttctttcgag tatatacaag      180

gtgattacat gtacgtttga agtacaactc tagattttgt agtgccctct tgggctagcg      240

gtaaaggtgc gcattttttc acaccctaca atgttctgtt caaaagattt tggtcaaacg      300

ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga aacttctccg cagtgaaaga      360

taaatgatct attagaacca gggaggtccg ttttagagct agaaatagca agttaaaata      420

aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtggtgctt tttttgtttt      480

ttatgtctag aaagcctgta tgcgaagcca caatcctttc caacagacca tactaagtat      540

tctttgtcat cagacaactt gttgagtggt actaaaggag tgcttttc                   588


<210>  53
<211>  419
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' fragment of the split SGIC DNA with 50 bp homology to the 3' 
       split SGIC DNA cassette for assembly

<400>  53
cttcatgcca gcaatagttg cgtgctgagc tcaacagtgc ccaacccttg aagcgacttc       60

caatcgcttt gcatatccag taccacaccc acaggcgttt tctttgaaaa gataatgtat      120

gattatgctt tcactcatat ttatacagaa acttgatgtt ttctttcgag tatatacaag      180

gtgattacat gtacgtttga agtacaactc tagattttgt agtgccctct tgggctagcg      240

gtaaaggtgc gcattttttc acaccctaca atgttctgtt caaaagattt tggtcaaacg      300

ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga aacttctccg cagtgaaaga      360

taaatgatct attagaacca gggaggtccg ttttagagct agaaatagca agttaaaat       419


<210>  54
<211>  249
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' fragment of the split SGIC DNA with 50 bp homology to the 5' 
       split SGIC DNA cassette for assembly.

<400>  54
aaacttctcc gcagtgaaag ataaatgatc tattagaacc agggaggtcc gttttagagc       60

tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt      120

cggtggtgct ttttttgttt tttatgtcta gaaagcctgt atgcgaagcc acaatccttt      180

ccaacagacc atactaagta ttctttgtca tcagacaact tgttgagtgg tactaaagga      240

gtgcttttc                                                              249


<210>  55
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ssODN 5' flank 1 kb upper strand sequence

<400>  55
cttcatgcca gcaatagttg cgtgctgagc tcaacagtgc ccaacccttg aagcgacttc       60

caatcgcttt gcatatccag taccacaccc acaggcgttt                            100


<210>  56
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ssODN 5' flank 1 kb lower strand sequence

<400>  56
aaacgcctgt gggtgtggta ctggatatgc aaagcgattg gaagtcgctt caagggttgg       60

gcactgttga gctcagcacg caactattgc tggcatgaag                            100


<210>  57
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ssODN 3' flank 1 kb upper strand sequence

<400>  57
agaaagcctg tatgcgaagc cacaatcctt tccaacagac catactaagt attctttgtc       60

atcagacaac ttgttgagtg gtactaaagg agtgcttttc                            100


<210>  58
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ssODN 3' flank 1 kb lower strand sequence

<400>  58
gaaaagcact cctttagtac cactcaacaa gttgtctgat gacaaagaat acttagtatg       60

gtctgttgga aaggattgtg gcttcgcata caggctttct                            100


<210>  59
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  connector sequence on the 5' end of SGIC DNA

<400>  59
aagcgacttc caatcgcttt gcatatccag taccacaccc acaggcgttt                  50


<210>  60
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  connector sequence on the 3' end of SGIC DNA

<400>  60
agaaagcctg tatgcgaagc cacaatcctt tccaacagac catactaagt                  50


<210>  61
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward PCR primer SGIC DNA part 5' fwnA flank-sgRNA-3' conH

<400>  61
gggggtctcg gtgcctgcga cagcggattg ggcggagaag aagacaaccc ttcagatata       60

ttcaggtgct tttccctcac atgttttg                                          88


<210>  62
<211>  87
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse PCR primer SGIC DNA part 5' fwnA flank-sgRNA-3' conH

<400>  62
cccggtctcc cattaatcgc aactcggatt tgggaggcaa ggtcggaacg cgaactttgg       60

ctttgagggt gcggaacatg gggactg                                           87


<210>  63
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward PCR primer SGIC DNA hygB or phleo marker-3' fnwA flank

<400>  63
gggggtctcg aatggctctg tacagtgacc ggtgac                                 36


<210>  64
<211>  86
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse PCR primer SGIC DNA hygB or phleo marker-3' fnwA flank

<400>  64
cccggtctcc gaggcagtca aagaatcgga cagtgcaagt tgcgtggtca gccgtcttct       60

tcccatcctg tcttcagtct taagac                                            86


<210>  65
<211>  18922
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BG-AMA5 AMA phleo/Cas9 st

<400>  65
cttgcccatc gaacgtacaa gtactcctct gttctctcct tcctttgctt tgtgcggaga       60

ccggcttact aaaagccaga taacagtatg catatttgcg cgctgatttt tgcggtataa      120

gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg      180

tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat      240

atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc      300

tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc      360

tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag      420

agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg      480

acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact      540

ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag      600

tgtgccggtt tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat      660

caaaaacgcc attaacctga tgttctgggg aatataaggt ctcgcctccg gatcgatgta      720

cacaaccgac tgcacccaaa cgaacacaaa tcttagcagt gccctcgccg gatagcttgg      780

actgtccttt accgtcgcca gcacaagaag ggtatctctg aggtccgtac cgccttttct      840

ttaccactgg attcgatttt cgcagttgga atgatacatc tggggactgc gaatggttta      900

cccctcggcc gatactatgg gtcgtgaaga gatggaacat tccgaaagtg ttttgcggat      960

aacattggtg gcatcgaaaa cagaatgctg accattgatt tcaacacgaa caggaggttg     1020

ccaagaagcg tacccgccgt gtcgtcaagt cccagcgtgc catcgtcggt gcttccctcg     1080

acgtgatcaa ggagcgccgc tcccagcgcc ccgaggcccg tgccgccgcc cgccagcagg     1140

ccatcaagga cgccaaggag aagaaggctg ccgctgagtc caagaagaag gctgagaagg     1200

ctaagaacgc cgctgctggt gccaagggtg ctgctcagcg catccagagc aagcagggtg     1260

ctaagggttc tgctcccaag gtcgctgcca agtctcgtta aggaatgaat aacggttcgg     1320

cttgggattg ggtgcggaag gcaagagttt catggacgaa ttttgggagg ttactggagc     1380

tggaatatgt gttttcccta ccaccaaaaa tgaaatgttc caaaactatc ggcgtgcaag     1440

acggcctctt acgggtttaa cggctctcag ataagctcta tcaatcgcgc cacggatgca     1500

tgaatgaaga tccagatggc cgcgggatat atcgtgctag tgtaattcct acatgatctt     1560

gctgttcact ccatgcgcat ccagatattc caggggtcga ctgttaattg atatgcctgg     1620

gcttgagact ccgtagacgc ccagtcaatg tgcaattaat acgagggtgc tgttatcggc     1680

agcaaccttg tacttctcca taagatgggg gaatgccatg gacctgagtg atcaattgac     1740

gcaagtctcc cataacgcgg cggcttgacc taaaatccat ataccgcccc gttgagcctc     1800

cgcgctccag agtcctgtcc cggaataggg cacaaaccta ggctaaccta attcgtcgtc     1860

cgcgtctgag ttcagacaaa agaacttcca agtatcagca gagtacgctg atattgataa     1920

gtaggcaaac ataagaccaa taagcaagta gaataaaaaa ttataaggac actgcctcca     1980

taaagcgccc tcccaagacc tcagggacaa aacttctcaa gtggcaattc actgcctcag     2040

gccgtgtcca gtgaagtgac gaagcgacac tgttgcctgc tgactcagcc gctttccgcc     2100

ctgccgaatt tgccatctcg cttacaggtc agcactagcg cgattcgccc acagatgctc     2160

agcgcaaagt ggtgactcag tcaaaccccc cctacaagat tccacctcga tttttcaact     2220

tcccatctcg atccgacaag ttctacatcc accgtcaaaa tggcctccag cgaagatgtc     2280

atcaaggagt tcatgcgctt caaggtccgc atggaaggat ccgtcaacgg ccacgagttc     2340

gagattgagg gtgagggtga gggccgcccc tacgaaggca cccagactgc caagctcaag     2400

gtcaccaagg gtggtcctct ccccttcgct tgggatatcc tgtctcctca gttccagtac     2460

ggctccaagg tctacgtcaa gcaccccgcc gacatccccg actacaagaa gctttctttc     2520

cccgagggtt tcaagtggga gcgtgtcatg aacttcgagg atggtggtgt tgtgaccgtt     2580

actcaggaca gcagcttgca ggatggctct ttcatctaca aggtcaagtt cattggtgtc     2640

aacttcccct ccgacggccc tgtcatgcag aagaagacca tgggctggga agcgtcgact     2700

gagcgtctgt acccccgtga cggtgttctc aagggtgaga tccacaaggc tctcaagctc     2760

aaggacggtg gtcactacct tgttgagttc aagtccatct acatggccaa gaagcctgtg     2820

cagctgcccg gatactacta cgtggactcc aagcttgaca tcacctccca caacgaagac     2880

tacaccattg ttgagcagta cgagcgtgct gagggccgcc accacctctt cctgacccac     2940

ggaatggatg agctgtacaa gtcgaaacta taaataaatg gtttgcgttg cgattgactg     3000

aaacgaaaaa aagcgaaaat gattctggga atgaattgat aaagcgcggg ctctgcggta     3060

cggttacggt tgcggtcgcg gacgaatgga ctgggctgag ctgggctgga ggaagtccat     3120

cgaacaagga caaggggtgg aatatggcac gggtcgattt tgttatacat accctaccat     3180

ccatctatcc atttaaatac caaatgagtt gttgaatgga ttcgcggtct tctcggttta     3240

tttttgcttg cttgcgtgct taagggatag tgtgcctcac gctttccggc atcttccaga     3300

ccacagtata tccatccgcc tcctgttgaa gcttattttt tgtatactgt tttgtgatag     3360

cacgaagttt ttccacggta tcttgttaaa aatatatatt tgtggcgggc ttacctacat     3420

caaattaata agagactaat tataaactaa acacacaagc aagctacttt agggtaaaag     3480

tttataaatg cttttgacgt ataaacgttg cttgtattta ttattacaat taaaggtgga     3540

tagaaaacct agagactagt tagaaactaa tctcaggttt gcgttaaact aaatcagagc     3600

ccgagaggtt aacagaacct agaaggggac tagatatccg ggtagggaaa caaaaaaaaa     3660

aaacaagaca gccacatatt agggagacta gttagaagct agttccagga ctaggaaaat     3720

aaaagacaat gataccacag tctagttgac aactagatag attctagatt gaggccaaag     3780

tctctgagat ccaggttagt tgcaactaat actagttagt atctagtctc ctataactct     3840

gaagctagaa taacttacta ctattatcct caccactgtt cagctgcgca aacggagtga     3900

ttgcaaggtg ttcagagact agttattgac tagtcagtga ctagcaataa ctaacaaggt     3960

attaacctac catgtctgcc atcaccctgc acttcctcgg gctcagcagc cttttcctcc     4020

tcattttcat gctcattttc cttgtttaag actgtgacta gtcaaagact agtccagaac     4080

cacaaaggag aaatgtctta ccactttctt cattgcttgt ctcttttgca ttatccatgt     4140

ctgcaactag ttagagtcta gttagtgact agtccgacga ggacttgctt gtctccggat     4200

tgttggagga actctccagg gcctcaagat ccacaacaga gccttctaga agactggtca     4260

ataactagtt ggtctttgtc tgagtctgac ttacgaggtt gcatactcgc tccctttgcc     4320

tcgtcaatcg atgagaaaaa gcgccaaaac tcgcaatatg gctttgaacc acacggtgct     4380

gagactagtt agaatctagt cccaaactag cttggatagc ttacctttgc cctttgcgtt     4440

gcgacaggtc ttgcagggta tggttccttt ctcaccagct gatttagctg ccttgctacc     4500

ctcacggcgg atctgcataa agagtggcta gaggttataa attagcactg atcctaggta     4560

cggggctgaa tgtaacttgc ctttcctttc tcatcgcgcg gcaagacagg cttgctcaaa     4620

ttcctaccag tcacaggggt atgcacggcg tacggaccac ttgaactagt cacagattag     4680

ttagcaacta gtctgcattg aatggctgta cttacgggcc ctcgccattg tcctgatcat     4740

ttccagcttc accctcgttg ctgcaaagta gttagtgact agtcaaggac tagttgaaat     4800

gggagaagaa actcacgaat tctcgacacc cttagtattg tggtccttgg acttggtgct     4860

gctatatatt agctaataca ctagttagac tcacagaaac ttacgcagct cgcttgcgct     4920

tcttggtagg agtcggggtt gggagaacag tgccttcaaa caagccttca taccatgcta     4980

cttgactagt cagggactag tcaccaagta atctagatag gacttgcctt tggcctccat     5040

cagttccttc atagtgggag gtccattgtg caatgtaaac tccatgccgt gggagttctt     5100

gtccttcaag tgcttgacca atatgtttct gttggcagag ggaacctgtc aactagttaa     5160

taactagtca gaaactagta tagcagtaga ctcactgtac gcttgaggca tcccttcact     5220

cggcagtaga cttcatatgg atggatatca ggcacgccat tgtcgtcctg tggactagtc     5280

agtaactagg cttaaagcta gtcgggtcgg cttactatct tgaaatccgg cagcgtaagc     5340

tccccgtcct taactgcctc gagatagtga cagtactctg gggactttcg gagatcgtta     5400

tcgcgaatgc tcggcatact aatcgttgac tagtcttgga ctagtcccga gcaaaaagga     5460

ttggaggagg aggaggaagg tgagagtgag acaaagagcg aaataagagc ttcaaaggct     5520

atctctaagc agtatgaagg ttaagtatct agttcttgac tagatttaaa agagatttcg     5580

actagttatg tacctggagt ttggatatag gaatgtgttg tggtaacgaa atgtaagggg     5640

gaggaaagaa aaagtcggtc aagaggtaac tctaagtcgg ccattccttt ttgggaggcg     5700

ctaaccataa acggcatggt cgacttagag ttagctcagg gaatttaggg agttatctgc     5760

gaccaccgag gaacggcgga atgccaaaga atcccgatgg agctctagct ggcggttgac     5820

aaccccacct tttggcgttt ctgcggcgtt gcaggcggga ctggatactt cgtagaacca     5880

gaaaggcaag gcagaacgcg ctcagcaaga gtgttggaag tgatagcatg atgtgccttg     5940

ttaactaggt caaaatctgc agtatgcttg atgttatcca aagtgtgaga gaggaaggtc     6000

caaacataca cgattgggag agggcctagg tataagagtt tttgagtaga acgcatgtga     6060

gcccagccat ctcgaggaga ttaaacacgg gccggcattt gatggctatg ttagtacccc     6120

aatggaaagc ctgagagtcc agtggtcgca gataactccc taaattccct gagctaactc     6180

taagtcgacc atgccgttta tggttagcgc ctcccaaaaa ggaatggccg acttagagtt     6240

acctcttgac cgactttttc tttcctcccc cttacatttc gttaccacaa cacattccta     6300

tatccaaact ccaggtacat aactagtcga aatctctttt aaatctagtc aagaactaga     6360

tacttaacct tcatactgct tagagatagc ctttgaagct cttatttcgc tctttgtctc     6420

actctcacct tcctcctcct cctccaatcc tttttgctcg ggactagtcc aagactagtc     6480

aacgattagt atgccgagca ttcgcgataa cgatctccga aagtccccag agtactgtca     6540

ctatctcgag gcagttaagg acggggagct tacgctgccg gatttcaaga tagtaagccg     6600

acccgactag ctttaagcct agttactgac tagtccacag gacgacaatg gcgtgcctga     6660

tatccatcca tatgaagtct actgccgagt gaagggatgc ctcaagcgta cagtgagtct     6720

actgctatac tagtttctga ctagttatta actagttgac aggttccctc tgccaacaga     6780

aacatattgg tcaagcactt gaaggacaag aactcccacg gcatggagtt tacattgcac     6840

aatggacctc ccactatgaa ggaactgatg gaggccaaag gcaagtccta tctagattac     6900

ttggtgacta gtccctgact agtcaagtag catggtatga aggcttgttt gaaggcactg     6960

ttctcccaac cccgactcct accaagaagc gcaagcgagc tgcgtaagtt tctgtgagtc     7020

taactagtgt attagctaat atatagcagc accaagtcca aggaccacaa tactaagggt     7080

gtcgagaatt cgtgagtttc ttctcccatt tcaactagtc cttgactagt cactaactac     7140

tttgcagcaa cgagggtgaa gctggaaatg atcaggacaa tggcgagggc ccgtaagtac     7200

agccattcaa tgcagactag ttgctaacta atctgtgact agttcaagtg gtccgtacgc     7260

cgtgcatacc cctgtgactg gtaggaattt gagcaagcct gtcttgccgc gcgatgagaa     7320

aggaaaggca agttacattc agccccgtac ctaggatcag tgctaattta taacctctag     7380

ccactcttta tgcagatccg ccgtgagggt agcaaggcag ctaaatcagc tggtgagaaa     7440

ggaaccatac cctgcaagac ctgtcgcaac gcaaagggca aaggtaagct atccaagcta     7500

gtttgggact agattctaac tagtctcagc accgtgtggt tcaaagccat attgcgagtt     7560

ttggcgcttt ttctcatcga ttgacgaggc aaagggagcg agtatgcaac ctcgtaagtc     7620

agactcagac aaagaccaac tagttattga ccagtcttct agaaggctct gttgtggatc     7680

ttgaggccct ggagagttcc tccaacaatc cggagacaag caagtcctcg tcggactagt     7740

cactaactag actctaacta gttgcagaca tggataatgc aaaagagaca agcaatgaag     7800

aaagtggtaa gacatttctc ctttgtggtt ctggactagt ctttgactag tcacagtctt     7860

aaacaaggaa aatgagcatg aaaatgagga ggaaaaggct gctgagcccg aggaagtgca     7920

gggtgatggc agacatggta ggttaatacc ttgttagtta ttgctagtca ctgactagtc     7980

aataactagt ctctgaacac cttgcaatca ctccgtttgc gcagctgaac agtggtgagg     8040

ataatagtag taagttattc tagcttcaga gttataggag actagatact aactagtatt     8100

agttgcaact aacctggatc tcagagactt tggcctcaat ctagaatcta tctagttgtc     8160

aactagactg tggtatcatt gtcttttatt ttcctagtcc tggaactagc ttctaactag     8220

tctccctaat atgtggctgt cttgtttttt ttttttgttt ccctacccgg atatctagtc     8280

cccttctagg ttctgttaac ctctcgggct ctgatttagt ttaacgcaaa cctgagatta     8340

gtttctaact agtctctagg ttttctatcc acctttaatt gtaataataa atacaagcaa     8400

cgtttatacg tcaaaagcat ttataaactt ttaccctaaa gtagcttgct tgtgtgttta     8460

gtttataatt agtctcttat taatttgatg taggtaagcc cgccacaaat atatattttt     8520

aacaagatac cgtggaaaaa cttcgtgcta tcacaaaaca gtatacaaaa aataagctat     8580

cgaattcctg cagagatcat cctgtcttca gtcttaagac ttctctccta tatcacccgc     8640

acttacccta gagtgccgct taggtgctaa gggcacattg agtattggcc gtgtagaata     8700

tatagcttaa gtacggccaa gcagacggga agccctgttc tccacaccct atggtcgtat     8760

atatcaggct tctaccggga aacgattaag agtgtataat ggactgaaaa tcaatatgaa     8820

cgggacaatg ctcaagttaa attagttagg catcctaatc tctactaaat gttctatcta     8880

gagatcgggg tactataggc ccgtacgtta atcactctac gcttctctcc cttaggtata     8940

gtgtaggtag gggctagaca tttatatgag tcagatggta caaacggtag gcagtgcggg     9000

cgaagaagtg aagacggagt cggttgaagc tacatacaaa agatgcattg gctcgtcatg     9060

aagagcctcc cgggtttagt cctgctcctc ggccacgaag tgcacgcagt tgccggccgg     9120

gtcgcgcagg gcgaactccc gcccccacgg ctgctcgccg atctcggtca tggccggccc     9180

ggaggcgtcc cggaagttcg tggacacgac ctccgaccac tcggcgtaca gctcgtccag     9240

gccgcgcacc cacacccagg ccagggtgtt gtccggcacc acctggtcct ggaccgcgct     9300

gatgaacagg gtcacgtcgt cccggaccac accggcgaag tcgtcctcca cgaagtcccg     9360

ggagaacccg agccggtcgg tccagaactc gaccgctccg gcgacgtcgc gcgcggtgag     9420

caccggaacg gcactggtca acttggccat tttgacggtg ggatcctgtg atgtctgctc     9480

aagcggggta gctgttagtc aagctgcgat gaagtgggaa agctcgaact gaaaggttca     9540

aaggaataag ggatgggaag gatggagtat ggatgtagca aagtacttac ttaggggaaa     9600

taaaggttct tggatgggaa gatgaatata ctgaagatgg gaaaagaaag agaaaagaaa     9660

agagcagctg gtggggagag caggaaaata tggcaacaaa tgttggactg acgcaacgac     9720

cttgtcaacc ccgccgacac accgggcgga cagacggggc aaagctgcct accagggact     9780

gagggacctc agcaggtcga gtgcagagca ccggatgggt cgactgccag cttgtgttcc     9840

cggtctgcgc cgctggccag ctcctgagcg gcctttccgg tttcatacac cgggcaaagc     9900

aggagaggca cgatatttgg acgccctaca gatgccggat gggccaatta gggagcttac     9960

gcgccgggta ctcgctctac ctacttcgga gaaggtacta tctcgtgaat cttttaccag    10020

atcggaagca attggacttc tgtacctagg ttaatggcat gctatttcgc cgacggctat    10080

acacccctgg cttcacattc tccttcgctt actgccggtg attcgatgaa gctccatatt    10140

ctccgatgat gcaatagatt cttggtcaac gaggggcaca ccagcctttc cacttcgggg    10200

cggaggggcg gccggtcccg gattaataat catccactgc acctcagagc cgccagagct    10260

gtctggcgca gtggcgctta ttactcagcc cttctctctg cgtccgtccg tctctccgca    10320

tgccagaaag agtcaccggt cactgtacag agcggccgcc accgcggtgg agctccaatt    10380

cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact    10440

gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct    10500

ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg    10560

gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca    10620

gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct    10680

ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt    10740

tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac    10800

gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct    10860

ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt    10920

ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac    10980

aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg    11040

gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc    11100

gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag    11160

tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt    11220

tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt    11280

gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga    11340

acgttttcca atgatgagca cttttcgacc gaataaatac ctgtgacgga agatcacttc    11400

gcagaataaa taaatcctgg tgtccctgtt gataccggga agccctgggc caacttttgg    11460

cgaaaatgag acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga    11520

tcactaccgg gcgtattttt tgagttgtcg agattttcag gagctaagga agctaaaatg    11580

gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat    11640

tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt    11700

acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac    11760

attcttgccc gcctgatgaa tgctcatccg gaattacgta tggcaatgaa agacggtgag    11820

ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg    11880

ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg    11940

caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat    12000

atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc    12060

aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac    12120

aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc    12180

ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt    12240

ttttaaggca gttattggtg cccttaaacg cctggttgct acgcctgaat aagtgataat    12300

aagcggatga atggcagaaa ttcgaaagca aattcgaccc ggtcgtcggt tcagggcagg    12360

gtcgttaaat agccgcttat gtctattgct ggtttaccgg tttattgact accggaagca    12420

gtgtgaccgt gtgcttctca aatgcctgag gccagtttgc tcaggctctc cccgtggagg    12480

taataattga cgatatgatc ctttttttct gatcaaaaag gatctaggtg aagatccttt    12540

ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc    12600

ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct    12660

tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa    12720

ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag    12780

tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc    12840

tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg    12900

actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca    12960

cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat    13020

gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg    13080

tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc    13140

ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc    13200

ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc    13260

cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg    13320

cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga    13380

gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc    13440

attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa    13500

ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg ctcccggctc    13560

gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg    13620

attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctgggt acgcaactct    13680

ctggaaatga aggcagcccc gaagttttgg gatactagcg atcctaggca ctgcaccagt    13740

cttgaagagg gtcatctctc cggagattag tccatctgtg gcattgttta tactttcaca    13800

cctccagaac aacatggaag tcaaggaatg tggtatcaga ctcacaacca agagatttct    13860

caccaaagcg ctagttccaa ggcaggtcta gcgtgctgac gatggggata atttagccgg    13920

ctaattggtg gacatccgcc accaccccag attaaacggt ggagatgaca gggggcggag    13980

attcaacggg attaaatatc ggagatgaag actcggcatc tgcttgaggc agttagtgct    14040

tgatgcaact tgtggtcggt cgaagcgatt ggcatggtga tcaacgatcg gataataaga    14100

cctcccatgt gcctcggggg atattcgatc cgcctgctga agagagtaat gatggacctg    14160

atacttgcag aatctgaact gaagcccttg actagcgctg gaactaaatt tcaagctaac    14220

ggtgatgcag cagaaggatg acgatctttt cctaacggat ttctccgcag acccccgagc    14280

gcattctgca ataccatgca cctttcatgc acctttcatg caagttccat gcaactccca    14340

cacatgtgca ttaatatgcc ttagctctct cgaatgaact ttcacgtggc ttaagtcccc    14400

tcacctgcac ccatataaaa gccaagttct tcccccacga tgacaccaac cccaactcac    14460

cttccaccgt caaaatggac aagaagtata gcatcggtct ggacattggc accaactccg    14520

ttggctgggc tgtcatcacc gacgagtaca aggttcctag caagaaattc aaggtcctgg    14580

gaaacaccga tcgtcactcc atcaagaaga acctcattgg tgcgcttctc ttcgactccg    14640

gtgagactgc tgaggccacc cgtctgaagc gtaccgctcg ccgtcgttac actcgccgca    14700

agaaccgtat ctgctacctc caggagattt tctccaacga gatggccaag gttgatgact    14760

ctttcttcca ccgtctggag gagtcgttcc ttgttgaaga agacaagaag cacgagcgtc    14820

accctatctt cggtaacatt gtcgatgagg tcgcttacca cgagaagtac cccaccatct    14880

accacctacg caaaaagctc gtcgacagca ccgacaaggc tgacctccgc ctcatttacc    14940

tggctctggc gcacatgatc aagttccgtg gtcacttcct gatcgagggt gacctcaacc    15000

ccgacaactc cgatgttgat aaactcttca tccagctcgt tcagacctac aaccagcttt    15060

tcgaggaaaa ccccatcaac gcgtctggcg tggatgccaa ggccatcctc tccgctcgcc    15120

tgagcaagtc ccgccgtctt gagaacttga ttgcccagct ccctggtgag aagaagaacg    15180

gtcttttcgg caaccttatt gccctgtccc tcggactgac tcccaacttc aagagcaact    15240

tcgatcttgc tgaggatgct aagttacaac tttccaagga tacctacgac gacgaccttg    15300

ataaccttct cgcccagata ggagatcagt acgccgacct cttcctagct gccaagaacc    15360

tctccgatgc cattctcctg tcagatatcc tccgtgtcaa cactgagatc accaaggccc    15420

ctctctccgc ctctatgatc aagagatacg atgagcacca ccaggacctc accctactca    15480

aggctctggt ccgccagcag ctccccgaaa agtacaagga gatcttcttc gaccagtcca    15540

agaacggcta cgccggttac atcgacggtg gtgcttccca ggaagaattc tacaagttca    15600

ttaagcctat cctcgagaag atggatggca ctgaggagct tcttgttaag ctgaaccgtg    15660

aggaccttct gcgcaagcag cgtactttcg acaacggcag catcccccac cagatccacc    15720

tgggtgaatt gcacgccatc cttcgtcgcc aggaagactt ctaccctttc ttgaaggaca    15780

accgtgagaa gattgagaag atcctgacct tccgtatccc ctactacgtc ggtcctctgg    15840

ctcgcggtaa ctcccgcttc gcctggatga cccgcaagtc cgaggaaacc atcaccccct    15900

ggaacttcga ggaagtcgtc gacaagggtg cctccgctca gagcttcatt gagcgtatga    15960

ccaacttcga caagaacctg cccaacgaga aagtcctgcc caagcactcc ctcttgtacg    16020

agtacttcac tgtctacaac gagctgacca aggtcaagta cgtgaccgag ggcatgcgca    16080

agcctgcttt cctctccggc gaacagaaga aggccattgt cgacctgctg ttcaagacta    16140

accgcaaggt gaccgtcaag cagctcaagg aagactactt caagaagatc gagtgctttg    16200

actccgttga gatctccggt gttgaggacc gcttcaacgc ttctctcggc acctaccacg    16260

atctgctcaa gatcatcaag gacaaggact tccttgacaa cgaggagaac gaagacattc    16320

ttgaggacat tgttcttacc ctcaccctct tcgaggaccg tgagatgatc gaagaacgtc    16380

tgaagaccta cgctcacctc ttcgacgaca aggtcatgaa gcagttgaag cgccgccgtt    16440

acactggctg gggtcgcctc tctcgcaagt tgattaacgg tatccgtgat aagcagtctg    16500

gcaagaccat ccttgacttc ctgaagtccg acggcttcgc caaccgcaac ttcatgcagc    16560

tcatccacga cgactctctg accttcaaag aggacatcca gaaggcccaa gtctccggcc    16620

agggtgactc gctacacgaa cacattgcca acctggctgg ttcccccgct atcaagaagg    16680

gtatcctgca gactgtgaag gttgttgacg agcttgtgaa ggtcatgggt cgtcacaagc    16740

ccgagaacat cgtcatcgaa atggctcgtg agaaccagac cactcagaag ggtcagaaga    16800

acagccgtga gcgcatgaag cgtatcgagg aaggcatcaa ggagctcggt tcccagattc    16860

tcaaggaaca ccccgtcgag aacacccagc tgcagaatga gaagctctac ctctactact    16920

tgcagaacgg acgtgacatg tacgtcgacc aggagctgga tatcaaccgc ctctccgact    16980

acgatgttga ccacatcgtc ccccagtcct tcctcaagga tgacagcatt gacaacaagg    17040

tgctcacccg ttccgacaag aatcgtggca agagcgataa cgtcccctcg gaagaggttg    17100

ttaagaagat gaagaactac tggagacaat tgctcaacgc taagctcatc actcagcgca    17160

agttcgacaa ccttaccaag gccgagcgtg gcggactctc cgagctcgac aaggccggtt    17220

tcatcaagcg tcaattggtt gaaacccgtc agatcactaa gcacgttgcc cagatcctgg    17280

actctcgcat gaacaccaag tacgacgaga acgacaagct catccgtgag gtcaaggtca    17340

tcaccttaaa gagcaagctg gtcagtgact ttaggaaaga cttccagttc tacaaggtcc    17400

gcgagatcaa caactaccac cacgctcacg atgcctacct caacgccgtc gtcggtactg    17460

ctttgattaa gaagtatccc aagctcgagt ccgagttcgt ctacggtgac tacaaggtgt    17520

acgacgtgcg caagatgatc gctaagtccg agcaggagat cggaaaggcc actgccaagt    17580

acttcttcta cagcaacatc atgaacttct tcaagaccga aataacattg gccaacggcg    17640

agattcgcaa gcgtcccttg attgagacta acggcgaaac cggtgagatc gtctgggaca    17700

agggccgtga cttcgctacc gtccgcaagg tcctttctat gccccaggtc aacattgtca    17760

agaagaccga ggtgcagact ggtggtttct ccaaggagtc gattcttccc aagcgcaact    17820

ccgacaagct gatcgctcgc aagaaggatt gggaccccaa gaagtacggt ggattcgatt    17880

cgcctaccgt tgcctactcc gtcttggttg tcgccaaggt cgagaagggc aagagcaaga    17940

agctgaagag tgtgaaggaa ctcctcggta tcaccatcat ggaacgcagc agcttcgaga    18000

agaaccctat cgacttcctg gaggccaagg gttacaaaga ggtcaagaag gacctcatca    18060

tcaagctccc caagtactct ctgttcgagc tggagaacgg ccgtaagcgc atgcttgctt    18120

ccgccggtga gctccagaag ggtaacgagc ttgccctccc ctccaagtac gtcaacttcc    18180

tctacctggc ctcccactac gagaagctca agggctctcc cgaggacaac gagcagaagc    18240

agctctttgt cgagcagcac aagcactacc tggatgagat catcgagcag atctccgagt    18300

tcagcaagcg tgtcatcctg gccgatgcca accttgacaa ggtcctctct gcctacaaca    18360

agcaccgtga caagcccatc cgcgagcagg cggagaacat catccacctg ttcaccctca    18420

ccaacctggg tgctcctgct gctttcaagt actttgacac caccatcgac cgcaagcgtt    18480

acacctccac caaggaagtg cttgatgcga ctctgatcca ccagtcgatt accggtctgt    18540

acgagactcg tatcgacctg tctcagctcg gtggtgactc tcgtgccgat cccaagaaga    18600

agcgcaaggt ttaaacaagc gcttagctcg aacaaaagaa aaagtaaaaa cggttaatag    18660

cattggattc cgaactacaa agtataaact agtttcactc cttgtagaag ccagatacgg    18720

gccggggtag ataccgcgca ctccctcagc agcctcgcag tcatggtcag catcgaagaa    18780

ctcccacaca aaatgcgctg ggagaagagt tcgaaatgca gctgggctca attagccgtc    18840

gtacacggag atagatactg aatacatacc cgtttgagcc tgaaaatttt tgacattcgt    18900

gcccatacca tgaacctcgt ac                                             18922


<210>  66
<211>  15186
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BG-AMA9 AMA hygB/Cas9 st./sgRNA cassette

<400>  66
ggtaccttgc ccatcgaacg tacaagtact cctctgttct ctccttcctt tgctttgtgc       60

ttttccctca catgttttgc cgcaccagcc atcccactat caaaaagcga tgatgtttga      120

gattgtcggg tgtccacatc ttttagtgtg aatcgctagt agaatttggg atattattga      180

gcatcatccc atgatagcga gtacaagccc cgagtaaata ccaacattgc tatgctgctg      240

tgctgctatc tagtttgcta cgttggtcgt tgacctcaca gggatttcca ccaaaaagtg      300

gaccgggcgg gcgccactcg gccgtgccac agcagcctga gagcggacaa ataacaacag      360

ccgcctgccg cggggttcgg ttgcaaacat gaccaacagg ccaggccatc atcaacccac      420

cgctgcgttg atgcccagga tttcagtcca ataatccaca atttaccaac ggatagagct      480

aggtgaatta gatagacagg agggccagag ggaggggacc gagatgaaaa attttcgatg      540

aaagagtggt caaggtgggg tcgtagttcg gcgctccgag ggcgaggaac caaggaaagg      600

cgaggaaagg acaggctgat cgcgctgcgt tgctgggctg caagcgtgtc cagttgagtc      660

tggaaaaggc tccgccgtga agattctgcg ttggtcccgc acctgcgcgg tgggggcatt      720

acccctccat gtccaatgat ttcaagtcaa agccaagggt tgaagcccgc ccgcttagtc      780

gccttctcgc ttgacccctc catataagta tttcccctcc tccccctccc acaaattttt      840

cctttccctt tcctccctcg tccgcttcag tacgtatatc ttccccccct ctctcttcct      900

tctcactctt ctctccttct ttcttgattc atcctctctc taactgactt ctttgctcag      960

cacctctacg cgttctggcc gtagtatctg agcaattttt ctacagactt tttctatcta     1020

attccaaaaa agaacttcga gttcattcac caccgtcaaa atgatctgac tgatgagtcc     1080

gtgaggacga aacgagtaag ctcgtctcag atatattcag tcactggttt tagagctaga     1140

aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt     1200

gcttttggcc ggcatggtcc cagcctcctc gctggcgccg gctgggcaac atgcttcggc     1260

atggcgaatg ggactaaaat gcgctaaact gggcttgact cagggaggga tcatggacta     1320

gccaattggg cgtgcacagc gcgactttgg agctggttct ggctcgcatg acttgtttcg     1380

tgctgcgggg gattccgttc ggacctgaca ttttaaaaat aaaaaatgga aacatcttga     1440

aagacaaaaa tgagtttcag tagtggtcta cagaccgtag ttttgttcct attcacagtg     1500

aaaataaggc gctgcaattg ctacgttcat aaatcgagta ttgttgtgct ccgaagcgcc     1560

agtccccatg ttccgcaccc tccggatcga tgtacacaac cgactgcacc caaacgaaca     1620

caaatcttag cagtgccctc gccggatagc ttggactgtc ctttaccgtc gccagcacaa     1680

gaagggtatc tctgaggtcc gtaccgcctt ttctttacca ctggattcga ttttcgcagt     1740

tggaatgata catctgggga ctgcgaatgg tttacccctc ggccgatact atgggtcgtg     1800

aagagatgga acattccgaa agtgttttgc ggataacatt ggtggcatcg aaaacagaat     1860

gctgaccatt gatttcaaca cgaacaggag gttgccaaga agcgtacccg ccgtgtcgtc     1920

aagtcccagc gtgccatcgt cggtgcttcc ctcgacgtga tcaaggagcg ccgctcccag     1980

cgccccgagg cccgtgccgc cgcccgccag caggccatca aggacgccaa ggagaagaag     2040

gctgccgctg agtccaagaa gaaggctgag aaggctaaga acgccgctgc tggtgccaag     2100

ggtgctgctc agcgcatcca gagcaagcag ggtgctaagg gttctgctcc caaggtcgct     2160

gccaagtctc gttaaggaat gaataacggt tcggcttggg attgggtgcg gaaggcaaga     2220

gtttcatgga cgaattttgg gaggttactg gagctggaat atgtgttttc cctaccacca     2280

aaaatgaaat gttccaaaac tatcggcgtg caagacggcc tcttacgggt ttaacggctc     2340

tcagataagc tctatcaatc gcgccacgga tgcatgaatg aagatccaga tggccgcggg     2400

atatatcgtg ctagtgtaat tcctacatga tcttgctgtt cactccatgc gcatccagat     2460

attccagggg tcgactgtta attgatatgc ctgggcttga gactccgtag acgcccagtc     2520

aatgtgcaat taatacgagg gtgctgttat cggcagcaac cttgtacttc tccataagat     2580

gggggaatgc catggacctg agtgatcaat tgacgcaagt ctcccataac gcggcggctt     2640

gacctaaaat ccatataccg ccccgttgag cctccgcgct ccagagtcct gtcccggaat     2700

agggcacaaa cctaggctaa cctaattcgt cgtccgcgtc tgagttcaga caaaagaact     2760

tccaagtatc agcagagtac gctgatattg ataagtaggc aaacataaga ccaataagca     2820

agtagaataa aaaattataa ggacactgcc tccataaagc gccctcccaa gacctcaggg     2880

acaaaacttc tcaagtggca attcactgcc tcaggccgtg tccagtgaag tgacgaagcg     2940

acactgttgc ctgctgactc agccgctttc cgccctgccg aatttgccat ctcgcttaca     3000

ggtcagcact agcgcgattc gcccacagat gctcagcgca aagtggtgac tcagtcaaac     3060

cccccctaca agattccacc tcgatttttc aacttcccat ctcgatccga caagttctac     3120

atccaccgtc aaaatggcct ccagcgaaga tgtcatcaag gagttcatgc gcttcaaggt     3180

ccgcatggaa ggatccgtca acggccacga gttcgagatt gagggtgagg gtgagggccg     3240

cccctacgaa ggcacccaga ctgccaagct caaggtcacc aagggtggtc ctctcccctt     3300

cgcttgggat atcctgtctc ctcagttcca gtacggctcc aaggtctacg tcaagcaccc     3360

cgccgacatc cccgactaca agaagctttc tttccccgag ggtttcaagt gggagcgtgt     3420

catgaacttc gaggatggtg gtgttgtgac cgttactcag gacagcagct tgcaggatgg     3480

ctctttcatc tacaaggtca agttcattgg tgtcaacttc ccctccgacg gccctgtcat     3540

gcagaagaag accatgggct gggaagcgtc gactgagcgt ctgtaccccc gtgacggtgt     3600

tctcaagggt gagatccaca aggctctcaa gctcaaggac ggtggtcact accttgttga     3660

gttcaagtcc atctacatgg ccaagaagcc tgtgcagctg cccggatact actacgtgga     3720

ctccaagctt gacatcacct cccacaacga agactacacc attgttgagc agtacgagcg     3780

tgctgagggc cgccaccacc tcttcctgac ccacggaatg gatgagctgt acaagtcgaa     3840

actataaata aatggtttgc gttgcgattg actgaaacga aaaaaagcga aaatgattct     3900

gggaatgaat tgataaagcg cgggctctgc ggtacggtta cggttgcggt cgcggacgaa     3960

tggactgggc tgagctgggc tggaggaagt ccatcgaaca aggacaaggg gtggaatatg     4020

gcacgggtcg attttgttat acatacccta ccatccatct atccatttaa ataccaaatg     4080

agttgttgaa tggattcgcg gtcttctcgg tttatttttg cttgcttgcg tgcttaaggg     4140

atagtgtgcc tcacgctttc cggcatcttc cagaccacag tatatccatc cgcctcctgt     4200

tgaagcttat tttttgtata ctgttttgtg atagcacgaa gtttttccac ggtatcttgt     4260

taaaaatata tatttgtggc gggcttacct acatcaaatt aataagagac taattataaa     4320

ctaaacacac aagcaagcta ctttagggta aaagtttata aatgcttttg acgtataaac     4380

gttgcttgta tttattatta caattaaagg tggatagaaa acctagagac tagttagaaa     4440

ctaatctcag gtttgcgtta aactaaatca gagcccgaga ggttaacaga acctagaagg     4500

ggactagata tccgggtagg gaaacaaaaa aaaaaaacaa gacagccaca tattagggag     4560

actagttaga agctagttcc aggactagga aaataaaaga caatgatacc acagtctagt     4620

tgacaactag atagattcta gattgaggcc aaagtctctg agatccaggt tagttgcaac     4680

taatactagt tagtatctag tctcctataa ctctgaagct agaataactt actactatta     4740

tcctcaccac tgttcagctg cgcaaacgga gtgattgcaa ggtgttcaga gactagttat     4800

tgactagtca gtgactagca ataactaaca aggtattaac ctaccatgtc tgccatcacc     4860

ctgcacttcc tcgggctcag cagccttttc ctcctcattt tcatgctcat tttccttgtt     4920

taagactgtg actagtcaaa gactagtcca gaaccacaaa ggagaaatgt cttaccactt     4980

tcttcattgc ttgtctcttt tgcattatcc atgtctgcaa ctagttagag tctagttagt     5040

gactagtccg acgaggactt gcttgtctcc ggattgttgg aggaactctc cagggcctca     5100

agatccacaa cagagccttc tagaagactg gtcaataact agttggtctt tgtctgagtc     5160

tgacttacga ggttgcatac tcgctccctt tgcctcgtca atcgatgaga aaaagcgcca     5220

aaactcgcaa tatggctttg aaccacacgg tgctgagact agttagaatc tagtcccaaa     5280

ctagcttgga tagcttacct ttgccctttg cgttgcgaca ggtcttgcag ggtatggttc     5340

ctttctcacc agctgattta gctgccttgc taccctcacg gcggatctgc ataaagagtg     5400

gctagaggtt ataaattagc actgatccta ggtacggggc tgaatgtaac ttgcctttcc     5460

tttctcatcg cgcggcaaga caggcttgct caaattccta ccagtcacag gggtatgcac     5520

ggcgtacgga ccacttgaac tagtcacaga ttagttagca actagtctgc attgaatggc     5580

tgtacttacg ggccctcgcc attgtcctga tcatttccag cttcaccctc gttgctgcaa     5640

agtagttagt gactagtcaa ggactagttg aaatgggaga agaaactcac gaattctcga     5700

cacccttagt attgtggtcc ttggacttgg tgctgctata tattagctaa tacactagtt     5760

agactcacag aaacttacgc agctcgcttg cgcttcttgg taggagtcgg ggttgggaga     5820

acagtgcctt caaacaagcc ttcataccat gctacttgac tagtcaggga ctagtcacca     5880

agtaatctag ataggacttg cctttggcct ccatcagttc cttcatagtg ggaggtccat     5940

tgtgcaatgt aaactccatg ccgtgggagt tcttgtcctt caagtgcttg accaatatgt     6000

ttctgttggc agagggaacc tgtcaactag ttaataacta gtcagaaact agtatagcag     6060

tagactcact gtacgcttga ggcatccctt cactcggcag tagacttcat atggatggat     6120

atcaggcacg ccattgtcgt cctgtggact agtcagtaac taggcttaaa gctagtcggg     6180

tcggcttact atcttgaaat ccggcagcgt aagctccccg tccttaactg cctcgagata     6240

gtgacagtac tctggggact ttcggagatc gttatcgcga atgctcggca tactaatcgt     6300

tgactagtct tggactagtc ccgagcaaaa aggattggag gaggaggagg aaggtgagag     6360

tgagacaaag agcgaaataa gagcttcaaa ggctatctct aagcagtatg aaggttaagt     6420

atctagttct tgactagatt taaaagagat ttcgactagt tatgtacctg gagtttggat     6480

ataggaatgt gttgtggtaa cgaaatgtaa gggggaggaa agaaaaagtc ggtcaagagg     6540

taactctaag tcggccattc ctttttggga ggcgctaacc ataaacggca tggtcgactt     6600

agagttagct cagggaattt agggagttat ctgcgaccac cgaggaacgg cggaatgcca     6660

aagaatcccg atggagctct agctggcggt tgacaacccc accttttggc gtttctgcgg     6720

cgttgcaggc gggactggat acttcgtaga accagaaagg caaggcagaa cgcgctcagc     6780

aagagtgttg gaagtgatag catgatgtgc cttgttaact aggtcaaaat ctgcagtatg     6840

cttgatgtta tccaaagtgt gagagaggaa ggtccaaaca tacacgattg ggagagggcc     6900

taggtataag agtttttgag tagaacgcat gtgagcccag ccatctcgag gagattaaac     6960

acgggccggc atttgatggc tatgttagta ccccaatgga aagcctgaga gtccagtggt     7020

cgcagataac tccctaaatt ccctgagcta actctaagtc gaccatgccg tttatggtta     7080

gcgcctccca aaaaggaatg gccgacttag agttacctct tgaccgactt tttctttcct     7140

cccccttaca tttcgttacc acaacacatt cctatatcca aactccaggt acataactag     7200

tcgaaatctc ttttaaatct agtcaagaac tagatactta accttcatac tgcttagaga     7260

tagcctttga agctcttatt tcgctctttg tctcactctc accttcctcc tcctcctcca     7320

atcctttttg ctcgggacta gtccaagact agtcaacgat tagtatgccg agcattcgcg     7380

ataacgatct ccgaaagtcc ccagagtact gtcactatct cgaggcagtt aaggacgggg     7440

agcttacgct gccggatttc aagatagtaa gccgacccga ctagctttaa gcctagttac     7500

tgactagtcc acaggacgac aatggcgtgc ctgatatcca tccatatgaa gtctactgcc     7560

gagtgaaggg atgcctcaag cgtacagtga gtctactgct atactagttt ctgactagtt     7620

attaactagt tgacaggttc cctctgccaa cagaaacata ttggtcaagc acttgaagga     7680

caagaactcc cacggcatgg agtttacatt gcacaatgga cctcccacta tgaaggaact     7740

gatggaggcc aaaggcaagt cctatctaga ttacttggtg actagtccct gactagtcaa     7800

gtagcatggt atgaaggctt gtttgaaggc actgttctcc caaccccgac tcctaccaag     7860

aagcgcaagc gagctgcgta agtttctgtg agtctaacta gtgtattagc taatatatag     7920

cagcaccaag tccaaggacc acaatactaa gggtgtcgag aattcgtgag tttcttctcc     7980

catttcaact agtccttgac tagtcactaa ctactttgca gcaacgaggg tgaagctgga     8040

aatgatcagg acaatggcga gggcccgtaa gtacagccat tcaatgcaga ctagttgcta     8100

actaatctgt gactagttca agtggtccgt acgccgtgca tacccctgtg actggtagga     8160

atttgagcaa gcctgtcttg ccgcgcgatg agaaaggaaa ggcaagttac attcagcccc     8220

gtacctagga tcagtgctaa tttataacct ctagccactc tttatgcaga tccgccgtga     8280

gggtagcaag gcagctaaat cagctggtga gaaaggaacc ataccctgca agacctgtcg     8340

caacgcaaag ggcaaaggta agctatccaa gctagtttgg gactagattc taactagtct     8400

cagcaccgtg tggttcaaag ccatattgcg agttttggcg ctttttctca tcgattgacg     8460

aggcaaaggg agcgagtatg caacctcgta agtcagactc agacaaagac caactagtta     8520

ttgaccagtc ttctagaagg ctctgttgtg gatcttgagg ccctggagag ttcctccaac     8580

aatccggaga caagcaagtc ctcgtcggac tagtcactaa ctagactcta actagttgca     8640

gacatggata atgcaaaaga gacaagcaat gaagaaagtg gtaagacatt tctcctttgt     8700

ggttctggac tagtctttga ctagtcacag tcttaaacaa ggaaaatgag catgaaaatg     8760

aggaggaaaa ggctgctgag cccgaggaag tgcagggtga tggcagacat ggtaggttaa     8820

taccttgtta gttattgcta gtcactgact agtcaataac tagtctctga acaccttgca     8880

atcactccgt ttgcgcagct gaacagtggt gaggataata gtagtaagtt attctagctt     8940

cagagttata ggagactaga tactaactag tattagttgc aactaacctg gatctcagag     9000

actttggcct caatctagaa tctatctagt tgtcaactag actgtggtat cattgtcttt     9060

tattttccta gtcctggaac tagcttctaa ctagtctccc taatatgtgg ctgtcttgtt     9120

tttttttttt gtttccctac ccggatatct agtccccttc taggttctgt taacctctcg     9180

ggctctgatt tagtttaacg caaacctgag attagtttct aactagtctc taggttttct     9240

atccaccttt aattgtaata ataaatacaa gcaacgttta tacgtcaaaa gcatttataa     9300

acttttaccc taaagtagct tgcttgtgtg tttagtttat aattagtctc ttattaattt     9360

gatgtaggta agcccgccac aaatatatat ttttaacaag ataccgtgga aaaacttcgt     9420

gctatcacaa aacagtatac aaaaaataag ctatcgaatt cctgcagaga tcatcctgtc     9480

ttcagtctta agacttctct cctatatcac ccgcacttac cctagagtgc cgcttaggtg     9540

ctaagggcac attgagtatt ggccgtgtag aatatatagc ttaagtacgg ccaagcagac     9600

gggaagccct gttctccaca ccctatggtc gtatatatca ggcttctacc gggaaacgat     9660

taagagtgta taatggactg aaaatcaata tgaacgggac aatgctcaag ttaaattagt     9720

taggcatcct aatctctact aaatgttcta tctagagatc ggggtactat aggcccgtac     9780

gttaatcact ctacgcttct ctcccttagg tatagtgtag gtaggggcta gacatttata     9840

tgagtcagat ggtacaaacg gtaggcagtg cgggcgaaga agtgaagacg gagtcggttg     9900

aagctacata caaaagatgc attggctcgt catgaagagc ctcccgggtt tattcctttg     9960

ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat    10020

cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg    10080

atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa    10140

ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg    10200

atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca    10260

accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct    10320

cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga    10380

aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat    10440

cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca    10500

catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt    10560

gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca    10620

tggcctccgc gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg    10680

tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt    10740

caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt    10800

tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga    10860

agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga    10920

acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcatt ttgacggtgg    10980

gatcctgtga tgtctgctca agcggggtag ctgttagtca agctgcgatg aagtgggaaa    11040

gctcgaactg aaaggttcaa aggaataagg gatgggaagg atggagtatg gatgtagcaa    11100

agtacttact taggggaaat aaaggttctt ggatgggaag atgaatatac tgaagatggg    11160

aaaagaaaga gaaaagaaaa gagcagctgg tggggagagc aggaaaatat ggcaacaaat    11220

gttggactga cgcaacgacc ttgtcaaccc cgccgacaca ccgggcggac agacggggca    11280

aagctgccta ccagggactg agggacctca gcaggtcgag tgcagagcac cggatgggtc    11340

gactgccagc ttgtgttccc ggtctgcgcc gctggccagc tcctgagcgg cctttccggt    11400

ttcatacacc gggcaaagca ggagaggcac gatatttgga cgccctacag atgccggatg    11460

ggccaattag ggagcttacg cgccgggtac tcgctctacc tacttcggag aaggtactat    11520

ctcgtgaatc ttttaccaga tcggaagcaa ttggacttct gtacctaggt taatggcatg    11580

ctatttcgcc gacggctata cacccctggc ttcacattct ccttcgctta ctgccggtga    11640

ttcgatgaag ctccatattc tccgatgatg caatagattc ttggtcaacg aggggcacac    11700

cagcctttcc acttcggggc ggaggggcgg ccggtcccgg attaataatc atccactgca    11760

cctcagagcc gccagagctg tctggcgcag tggcgcttat tactcagccc ttctctctgc    11820

gtccgtccgt ctctccgcat gccagaaaga gtcaccggtc actgtacaga gcggccgcca    11880

ccgcggtgga gctccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc    11940

gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca    12000

catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa    12060

cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg    12120

ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct    12180

ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat    12240

cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt    12300

gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg    12360

acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac    12420

cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta    12480

aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca    12540

atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa    12600

tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt    12660

gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg    12720

cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag    12780

atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg    12840

agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttcgaccg aataaatacc    12900

tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg ataccgggaa    12960

gccctgggcc aacttttggc gaaaatgaga cgttgatcgg cacgtaagag gttccaactt    13020

tcaccataat gaaataagat cactaccggg cgtatttttt gagttgtcga gattttcagg    13080

agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca    13140

atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca    13200

gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt    13260

ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattacgtat    13320

ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt    13380

ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca    13440

gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc    13500

taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag    13560

ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa    13620

atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt    13680

ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg    13740

gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggttgcta    13800

cgcctgaata agtgataata agcggatgaa tggcagaaat tcgaaagcaa attcgacccg    13860

gtcgtcggtt cagggcaggg tcgttaaata gccgcttatg tctattgctg gtttaccggt    13920

ttattgacta ccggaagcag tgtgaccgtg tgcttctcaa atgcctgagg ccagtttgct    13980

caggctctcc ccgtggaggt aataattgac gatatgatcc tttttttctg atcaaaaagg    14040

atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg    14100

ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt    14160

ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg    14220

ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata    14280

ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca    14340

ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag    14400

tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc    14460

tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga    14520

tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg    14580

tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac    14640

gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg    14700

tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg    14760

ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct    14820

gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc    14880

gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc    14940

cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg    15000

ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta    15060

cactttatgc tcccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca    15120

ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa    15180

aagctg                                                               15186


<210>  67
<211>  3519
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TOPO Zero Blunt cloning vector

<400>  67
agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc       60

acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc      120

tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa      180

ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat      240

ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca      300

ctagtaacgg ccgccagtgt gctggaattc gcccttaagg gcgaattctg cagatatcca      360

tcacactggc ggccgctcga gcatgcatct agagggccca attcgcccta tagtgagtcg      420

tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc      480

caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc      540

cgcaccgatc gcccttccca acagttgcgc agcctatacg tacggcagtt taaggtttac      600

acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac      660

acgccggggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc      720

tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc      780

gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc      840

gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggcatg      900

agattatcaa aaaggatctt cacctagatc cttttcacgt agaaagccag tccgcagaaa      960

cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc     1020

gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt     1080

ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag     1140

ccctgcaaag taaactggat ggctttctcg ccgccaagga tctgatggcg caggggatca     1200

agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac     1260

gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca     1320

atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt     1380

gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg     1440

tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga     1500

agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct     1560

cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg     1620

gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg     1680

gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc     1740

gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat     1800

ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac     1860

tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt     1920

gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct     1980

cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg aattattaac     2040

gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc     2100

atacaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa     2160

tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatagc     2220

acgtgaggag ggccaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg     2280

acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg     2340

aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg     2400

accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt     2460

acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga     2520

ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact     2580

gcgtgcactt cgtggccgag gagcaggact gacacgtgct aaaacttcat ttttaattta     2640

aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt     2700

tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt     2760

tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt     2820

gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc     2880

agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg     2940

tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg     3000

ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt     3060

cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac     3120

tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg     3180

acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg     3240

gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat     3300

ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt     3360

tacggttcct gggcttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg     3420

attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa     3480

cgaccgagcg cagcgagtca gtgagcgagg aagcggaag                            3519


<210>  68
<211>  3506
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Backbone vector AB

<400>  68
tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata       60

ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat      120

aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct      180

attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact      240

gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag      300

ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc      360

gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgag      420

tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat      480

tcttctaata cctggaacgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca      540

tcaggagtac ggataaaatg cttgatggtc ggaagtggca taaattccgt cagccagttt      600

agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac      660

aactctggcg catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca      720

ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc      780

ctcgacgttt cccgttgaat atggctcata ttcttccttt ttcaatatta ttgaagcatt      840

tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa      900

ataggggtca gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata      960

cctgaatatg gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc     1020

tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc     1080

ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact     1140

gggcctttcg cccgggctaa ttagggggtg tcgcccttat tcgactctat agtgaagttc     1200

ctattctcta gaaagtatag gaacttctga agtggggttg cccatcgaac gtacaagtac     1260

tcctctgttc tctccttcct ttgctttgtg cggagaccgg cttactaaaa gccagataac     1320

agtatgcata tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc     1380

gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga     1440

cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc     1500

atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg     1560

atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc     1620

tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt     1680

ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag     1740

tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga     1800

tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtttccg ttatcgggga     1860

agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt     1920

ctggggaata taaggtctcg cctccggatc gatgtacaca accgactgca cccaaacgaa     1980

cacaaatctt agcaaaaatg aagtgaagtt cctatacttt ctagagaata ggaacttcta     2040

tagtgagtcg aataagggcg acacaaaatt tattctaaat gcataataaa tactgataac     2100

atcttatagt ttgtattata ttttgtatta tcgttgacat gtataatttt gatatcaaaa     2160

actgattttc cctttattat tttcgagatt tattttctta attctcttta acaaactaga     2220

aatattgtat atacaaaaaa tcataaataa tagatgaata gtttaattat aggtgttcat     2280

caatcgaaaa agcaacgtat cttatttaaa gtgcgttgct tttttctcat ttataaggtt     2340

aaataattct catatatcaa gcaaagtgac aggcgccctt aaatattctg acaaatgctc     2400

tttccctaaa ctccccccat aaaaaaaccc gccgaagcgg gtttttacgt tatttgcgga     2460

ttaacgatta ctcgttatca gaaccgccca gggggcccga gcttaagact ggccgtcgtt     2520

ttacaacaca gaaagagttt gtagaaacgc aaaaaggcca tccgtcaggg gccttctgct     2580

tagtttgatg cctggcagtt ccctactctc gccttccgct tcctcgctca ctgactcgct     2640

gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt     2700

atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc     2760

caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga     2820

gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata     2880

ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac     2940

cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg     3000

taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc     3060

cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag     3120

acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt     3180

aggcggtgct acagagttct tgaagtggtg ggctaactac ggctacacta gaagaacagt     3240

atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg     3300

atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac     3360

gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca     3420

gtggaacgac gcgcgcgtaa ctcacgttaa gggattttgg tcatgagctt gcgccgtccc     3480

gtcaagtcag cgtaatgctc tgcttt                                          3506


<210>  69
<211>  6938
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector SGIC DNA hygB

<400>  69
tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata       60

ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat      120

aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct      180

attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact      240

gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag      300

ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc      360

gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgag      420

tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat      480

tcttctaata cctggaacgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca      540

tcaggagtac ggataaaatg cttgatggtc ggaagtggca taaattccgt cagccagttt      600

agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac      660

aactctggcg catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca      720

ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc      780

ctcgacgttt cccgttgaat atggctcata ttcttccttt ttcaatatta ttgaagcatt      840

tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa      900

ataggggtca gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata      960

cctgaatatg gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc     1020

tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc     1080

ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact     1140

gggcctttcg cccgggctaa ttagggggtg tcgcccttat tcgactctat agtgaagttc     1200

ctattctcta gaaagtatag gaacttctga agtggggttg cccatcgaac gtacaagtac     1260

tcctctgttc tctccttcct ttgctttgtg cctgcgacag cggattgggc ggagaagaag     1320

acaacccttc agatatattc aggtgctttt ccctcacatg ttttgccgca ccagccatcc     1380

cactatcaaa aagcgatgat gtttgagatt gtcgggtgtc cacatctttt agtgtgaatc     1440

gctagtagaa tttgggatat tattgagcat catcccatga tagcgagtac aagccccgag     1500

taaataccaa cattgctatg ctgctgtgct gctatctagt ttgctacgtt ggtcgttgac     1560

ctcacaggga tttccaccaa aaagtggacc gggcgggcgc cactcggccg tgccacagca     1620

gcctgagagc ggacaaataa caacagccgc ctgccgcggg gttcggttgc aaacatgacc     1680

aacaggccag gccatcatca acccaccgct gcgttgatgc ccaggatttc agtccaataa     1740

tccacaattt accaacggat agagctaggt gaattagata gacaggaggg ccagagggag     1800

gggaccgaga tgaaaaattt tcgatgaaag agtggtcaag gtggggtcgt agttcggcgc     1860

tccgagggcg aggaaccaag gaaaggcgag gaaaggacag gctgatcgcg ctgcgttgct     1920

gggctgcaag cgtgtccagt tgagtctgga aaaggctccg ccgtgaagat tctgcgttgg     1980

tcccgcacct gcgcggtggg ggcattaccc ctccatgtcc aatgatttca agtcaaagcc     2040

aagggttgaa gcccgcccgc ttagtcgcct tctcgcttga cccctccata taagtatttc     2100

ccctcctccc cctcccacaa atttttcctt tccctttcct ccctcgtccg cttcagtacg     2160

tatatcttcc ccccctctct cttccttctc actcttctct ccttctttct tgattcatcc     2220

tctctctaac tgacttcttt gctcagcacc tctacgcgtt ctggccgtag tatctgagca     2280

atttttctac agactttttc tatctaattc caaaaaagaa cttcgagttc attcaccacc     2340

gtcaaaatga tctgactgat gagtccgtga ggacgaaacg agtaagctcg tctcagatat     2400

attcagtcac tggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca     2460

acttgaaaaa gtggcaccga gtcggtgctt ttggccggca tggtcccagc ctcctcgctg     2520

gcgccggctg ggcaacatgc ttcggcatgg cgaatgggac taaaatgcgc taaactgggc     2580

ttgactcagg gagggatcat ggactagcca attgggcgtg cacagcgcga ctttggagct     2640

ggttctggct cgcatgactt gtttcgtgct gcgggggatt ccgttcggac ctgacatttt     2700

aaaaataaaa aatggaaaca tcttgaaaga caaaaatgag tttcagtagt ggtctacaga     2760

ccgtagtttt gttcctattc acagtgaaaa taaggcgctg caattgctac gttcataaat     2820

cgagtattgt tgtgctccga agcgccagtc cccatgttcc gcaccctcaa agccaaagtt     2880

cgcgttccga ccttgcctcc caaatccgag ttgcgattaa tggctctgta cagtgaccgg     2940

tgactctttc tggcatgcgg agagacggac ggacgcagag agaagggctg agtaataagc     3000

gccactgcgc cagacagctc tggcggctct gaggtgcagt ggatgattat taatccggga     3060

ccggccgccc ctccgccccg aagtggaaag gctggtgtgc ccctcgttga ccaagaatct     3120

attgcatcat cggagaatat ggagcttcat cgaatcaccg gcagtaagcg aaggagaatg     3180

tgaagccagg ggtgtatagc cgtcggcgaa atagcatgcc attaacctag gtacagaagt     3240

ccaattgctt ccgatctggt aaaagattca cgagatagta ccttctccga agtaggtaga     3300

gcgagtaccc ggcgcgtaag ctccctaatt ggcccatccg gcatctgtag ggcgtccaaa     3360

tatcgtgcct ctcctgcttt gcccggtgta tgaaaccgga aaggccgctc aggagctggc     3420

cagcggcgca gaccgggaac acaagctggc agtcgaccca tccggtgctc tgcactcgac     3480

ctgctgaggt ccctcagtcc ctggtaggca gctttgcccc gtctgtccgc ccggtgtgtc     3540

ggcggggttg acaaggtcgt tgcgtcagtc caacatttgt tgccatattt tcctgctctc     3600

cccaccagct gctcttttct tttctctttc ttttcccatc ttcagtatat tcatcttccc     3660

atccaagaac ctttatttcc cctaagtaag tactttgcta catccatact ccatccttcc     3720

catcccttat tcctttgaac ctttcagttc gagctttccc acttcatcgc agcttgacta     3780

acagctaccc cgcttgagca gacatcacag gatcccaccg tcaaaatgcc tgaactcacc     3840

gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag     3900

ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc     3960

ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt     4020

gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg     4080

acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa     4140

ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt     4200

agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg     4260

cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac     4320

gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac     4380

tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac     4440

aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac     4500

gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc     4560

tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc     4620

cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct     4680

tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca     4740

caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat     4800

agtggaaacc gacgccccag cactcgtccg agggcaaagg aataaacccg ggaggctctt     4860

catgacgagc caatgcatct tttgtatgta gcttcaaccg actccgtctt cacttcttcg     4920

cccgcactgc ctaccgtttg taccatctga ctcatataaa tgtctagccc ctacctacac     4980

tatacctaag ggagagaagc gtagagtgat taacgtacgg gcctatagta ccccgatctc     5040

tagatagaac atttagtaga gattaggatg cctaactaat ttaacttgag cattgtcccg     5100

ttcatattga ttttcagtcc attatacact cttaatcgtt tcccggtaga agcctgatat     5160

atacgaccat agggtgtgga gaacagggct tcccgtctgc ttggccgtac ttaagctata     5220

tattctacac ggccaatact caatgtgccc ttagcaccta agcggcactc tagggtaagt     5280

gcgggtgata taggagagaa gtcttaagac tgaagacagg atgggaagaa gacggctgac     5340

cacgcaactt gcactgtccg attctttgac tgcctccgga tcgatgtaca caaccgactg     5400

cacccaaacg aacacaaatc ttagcaaaaa tgaagtgaag ttcctatact ttctagagaa     5460

taggaacttc tatagtgagt cgaataaggg cgacacaaaa tttattctaa atgcataata     5520

aatactgata acatcttata gtttgtatta tattttgtat tatcgttgac atgtataatt     5580

ttgatatcaa aaactgattt tccctttatt attttcgaga tttattttct taattctctt     5640

taacaaacta gaaatattgt atatacaaaa aatcataaat aatagatgaa tagtttaatt     5700

ataggtgttc atcaatcgaa aaagcaacgt atcttattta aagtgcgttg cttttttctc     5760

atttataagg ttaaataatt ctcatatatc aagcaaagtg acaggcgccc ttaaatattc     5820

tgacaaatgc tctttcccta aactcccccc ataaaaaaac ccgccgaagc gggtttttac     5880

gttatttgcg gattaacgat tactcgttat cagaaccgcc cagggggccc gagcttaaga     5940

ctggccgtcg ttttacaaca cagaaagagt ttgtagaaac gcaaaaaggc catccgtcag     6000

gggccttctg cttagtttga tgcctggcag ttccctactc tcgccttccg cttcctcgct     6060

cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc     6120

ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg     6180

ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg     6240

cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg     6300

actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac     6360

cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca     6420

tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt     6480

gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc     6540

caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag     6600

agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tgggctaact acggctacac     6660

tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt     6720

tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa     6780

gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg     6840

gtctgacgct cagtggaacg acgcgcgcgt aactcacgtt aagggatttt ggtcatgagc     6900

ttgcgccgtc ccgtcaagtc agcgtaatgc tctgcttt                             6938


<210>  70
<211>  6293
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector SGIC DNA phleo

<400>  70
tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata       60

ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat      120

aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct      180

attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact      240

gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag      300

ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc      360

gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgag      420

tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat      480

tcttctaata cctggaacgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca      540

tcaggagtac ggataaaatg cttgatggtc ggaagtggca taaattccgt cagccagttt      600

agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac      660

aactctggcg catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca      720

ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc      780

ctcgacgttt cccgttgaat atggctcata ttcttccttt ttcaatatta ttgaagcatt      840

tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa      900

ataggggtca gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata      960

cctgaatatg gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc     1020

tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc     1080

ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact     1140

gggcctttcg cccgggctaa ttagggggtg tcgcccttat tcgactctat agtgaagttc     1200

ctattctcta gaaagtatag gaacttctga agtggggttg cccatcgaac gtacaagtac     1260

tcctctgttc tctccttcct ttgctttgtg cctgcgacag cggattgggc ggagaagaag     1320

acaacccttc agatatattc aggtgctttt ccctcacatg ttttgccgca ccagccatcc     1380

cactatcaaa aagcgatgat gtttgagatt gtcgggtgtc cacatctttt agtgtgaatc     1440

gctagtagaa tttgggatat tattgagcat catcccatga tagcgagtac aagccccgag     1500

taaataccaa cattgctatg ctgctgtgct gctatctagt ttgctacgtt ggtcgttgac     1560

ctcacaggga tttccaccaa aaagtggacc gggcgggcgc cactcggccg tgccacagca     1620

gcctgagagc ggacaaataa caacagccgc ctgccgcggg gttcggttgc aaacatgacc     1680

aacaggccag gccatcatca acccaccgct gcgttgatgc ccaggatttc agtccaataa     1740

tccacaattt accaacggat agagctaggt gaattagata gacaggaggg ccagagggag     1800

gggaccgaga tgaaaaattt tcgatgaaag agtggtcaag gtggggtcgt agttcggcgc     1860

tccgagggcg aggaaccaag gaaaggcgag gaaaggacag gctgatcgcg ctgcgttgct     1920

gggctgcaag cgtgtccagt tgagtctgga aaaggctccg ccgtgaagat tctgcgttgg     1980

tcccgcacct gcgcggtggg ggcattaccc ctccatgtcc aatgatttca agtcaaagcc     2040

aagggttgaa gcccgcccgc ttagtcgcct tctcgcttga cccctccata taagtatttc     2100

ccctcctccc cctcccacaa atttttcctt tccctttcct ccctcgtccg cttcagtacg     2160

tatatcttcc ccccctctct cttccttctc actcttctct ccttctttct tgattcatcc     2220

tctctctaac tgacttcttt gctcagcacc tctacgcgtt ctggccgtag tatctgagca     2280

atttttctac agactttttc tatctaattc caaaaaagaa cttcgagttc attcaccacc     2340

gtcaaaatga tctgactgat gagtccgtga ggacgaaacg agtaagctcg tctcagatat     2400

attcagtcac tggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca     2460

acttgaaaaa gtggcaccga gtcggtgctt ttggccggca tggtcccagc ctcctcgctg     2520

gcgccggctg ggcaacatgc ttcggcatgg cgaatgggac taaaatgcgc taaactgggc     2580

ttgactcagg gagggatcat ggactagcca attgggcgtg cacagcgcga ctttggagct     2640

ggttctggct cgcatgactt gtttcgtgct gcgggggatt ccgttcggac ctgacatttt     2700

aaaaataaaa aatggaaaca tcttgaaaga caaaaatgag tttcagtagt ggtctacaga     2760

ccgtagtttt gttcctattc acagtgaaaa taaggcgctg caattgctac gttcataaat     2820

cgagtattgt tgtgctccga agcgccagtc cccatgttcc gcaccctcaa agccaaagtt     2880

cgcgttccga ccttgcctcc caaatccgag ttgcgattaa tggctctgta cagtgaccgg     2940

tgactctttc tggcatgcgg agagacggac ggacgcagag agaagggctg agtaataagc     3000

gccactgcgc cagacagctc tggcggctct gaggtgcagt ggatgattat taatccggga     3060

ccggccgccc ctccgccccg aagtggaaag gctggtgtgc ccctcgttga ccaagaatct     3120

attgcatcat cggagaatat ggagcttcat cgaatcaccg gcagtaagcg aaggagaatg     3180

tgaagccagg ggtgtatagc cgtcggcgaa atagcatgcc attaacctag gtacagaagt     3240

ccaattgctt ccgatctggt aaaagattca cgagatagta ccttctccga agtaggtaga     3300

gcgagtaccc ggcgcgtaag ctccctaatt ggcccatccg gcatctgtag ggcgtccaaa     3360

tatcgtgcct ctcctgcttt gcccggtgta tgaaaccgga aaggccgctc aggagctggc     3420

cagcggcgca gaccgggaac acaagctggc agtcgaccca tccggtgctc tgcactcgac     3480

ctgctgaggt ccctcagtcc ctggtaggca gctttgcccc gtctgtccgc ccggtgtgtc     3540

ggcggggttg acaaggtcgt tgcgtcagtc caacatttgt tgccatattt tcctgctctc     3600

cccaccagct gctcttttct tttctctttc ttttcccatc ttcagtatat tcatcttccc     3660

atccaagaac ctttatttcc cctaagtaag tactttgcta catccatact ccatccttcc     3720

catcccttat tcctttgaac ctttcagttc gagctttccc acttcatcgc agcttgacta     3780

acagctaccc cgcttgagca gacatcacag gatcccaccg tcaaaatggc caagttgacc     3840

agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac     3900

cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac     3960

gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg     4020

gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac     4080

ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag     4140

ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactaa     4200

acccgggagg ctcttcatga cgagccaatg catcttttgt atgtagcttc aaccgactcc     4260

gtcttcactt cttcgcccgc actgcctacc gtttgtacca tctgactcat ataaatgtct     4320

agcccctacc tacactatac ctaagggaga gaagcgtaga gtgattaacg tacgggccta     4380

tagtaccccg atctctagat agaacattta gtagagatta ggatgcctaa ctaatttaac     4440

ttgagcattg tcccgttcat attgattttc agtccattat acactcttaa tcgtttcccg     4500

gtagaagcct gatatatacg accatagggt gtggagaaca gggcttcccg tctgcttggc     4560

cgtacttaag ctatatattc tacacggcca atactcaatg tgcccttagc acctaagcgg     4620

cactctaggg taagtgcggg tgatatagga gagaagtctt aagactgaag acaggatggg     4680

aagaagacgg ctgaccacgc aacttgcact gtccgattct ttgactgcct ccggatcgat     4740

gtacacaacc gactgcaccc aaacgaacac aaatcttagc aaaaatgaag tgaagttcct     4800

atactttcta gagaatagga acttctatag tgagtcgaat aagggcgaca caaaatttat     4860

tctaaatgca taataaatac tgataacatc ttatagtttg tattatattt tgtattatcg     4920

ttgacatgta taattttgat atcaaaaact gattttccct ttattatttt cgagatttat     4980

tttcttaatt ctctttaaca aactagaaat attgtatata caaaaaatca taaataatag     5040

atgaatagtt taattatagg tgttcatcaa tcgaaaaagc aacgtatctt atttaaagtg     5100

cgttgctttt ttctcattta taaggttaaa taattctcat atatcaagca aagtgacagg     5160

cgcccttaaa tattctgaca aatgctcttt ccctaaactc cccccataaa aaaacccgcc     5220

gaagcgggtt tttacgttat ttgcggatta acgattactc gttatcagaa ccgcccaggg     5280

ggcccgagct taagactggc cgtcgtttta caacacagaa agagtttgta gaaacgcaaa     5340

aaggccatcc gtcaggggcc ttctgcttag tttgatgcct ggcagttccc tactctcgcc     5400

ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc     5460

agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa     5520

catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt     5580

tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg     5640

gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg     5700

ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag     5760

cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc     5820

caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa     5880

ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg     5940

taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtgggc     6000

taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac     6060

cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg     6120

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt     6180

gatcttttct acggggtctg acgctcagtg gaacgacgcg cgcgtaactc acgttaaggg     6240

attttggtca tgagcttgcg ccgtcccgtc aagtcagcgt aatgctctgc ttt            6293


<210>  71
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse PCR primer SGIC fragment I

<400>  71
cagtcaaaga atcggacagt gcaagttgcg tggtcagccg tcttcttccc gagggtgcgg       60


<210>  72
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward PCR primer SGIC fragment II and III

<400>  72
ctgcgacagc ggattgggcg gag                                               23


<210>  73
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse PCR primer SGIC fragment II and IV

<400>  73
cagtcaaaga atcggacagt gc                                                22


<210>  74
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse PCR primer SGIC fragment III

<400>  74
aatcgcaact cggatttggg                                                   20


<210>  75
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward PCR primer SGIC fragment IV

<400>  75
aaagccaaag ttcgcgttcc                                                   20


<210>  76
<211>  5174
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TOPO SGIC DNA sgRNA fwnA

<400>  76
aagggcgaat tctgcagata tccatcacac tggcggccgc tcgagcatgc atctagaggg       60

cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt      120

gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc      180

agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagccta      240

tacgtacggc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg      300

gatgtacaga gtgatattat tgacacgccg gggcgacgga tggtgatccc cctggccagt      360

gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat      420

gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa      480

gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc      540

tggggaatat aaatgtcagg catgagatta tcaaaaagga tcttcaccta gatccttttc      600

acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct      660

atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca      720

tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg      780

gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt ctcgccgcca      840

aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc      900

atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc      960

ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca     1020

gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg     1080

caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg     1140

ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag     1200

gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg     1260

cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc     1320

atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa     1380

gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac     1440

ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat     1500

ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac     1560

atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc     1620

ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt     1680

gacgagttct tctgaattat taacgcttac aatttcctga tgcggtattt tctccttacg     1740

catctgtgcg gtatttcaca ccgcatacag gtggcacttt tcggggaaat gtgcgcggaa     1800

cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac     1860

cctgataaat gcttcaataa tagcacgtga ggagggccac catggccaag ttgaccagtg     1920

ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc     1980

tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga     2040

ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt     2100

gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc     2160

gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg     2220

ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg     2280

tgctaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca     2340

tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga     2400

tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa     2460

aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga     2520

aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt     2580

taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt     2640

taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat     2700

agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct     2760

tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca     2820

cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag     2880

agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc     2940

gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga     3000

aaaacgccag caacgcggcc tttttacggt tcctgggctt ttgctggcct tttgctcaca     3060

tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag     3120

ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg     3180

aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct     3240

ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt     3300

agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg     3360

gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc     3420

tatttaggtg acactataga atactcaagc tatgcatcaa gcttggtacc gagctcggat     3480

ccactagtaa cggccgccag tgtgctggaa ttcgcccttg ggggtctcgg tgcctgcgac     3540

agcggattgg gcggagaaga agacaaccct tcagatatat tcaggtgctt ttccctcaca     3600

tgttttgccg caccagccat cccactatca aaaagcgatg atgtttgaga ttgtcgggtg     3660

tccacatctt ttagtgtgaa tcgctagtag aatttgggat attattgagc atcatcccat     3720

gatagcgagt acaagccccg agtaaatacc aacattgcta tgctgctgtg ctgctatcta     3780

gtttgctacg ttggtcgttg acctcacagg gatttccacc aaaaagtgga ccgggcgggc     3840

gccactcggc cgtgccacag cagcctgaga gcggacaaat aacaacagcc gcctgccgcg     3900

gggttcggtt gcaaacatga ccaacaggcc aggccatcat caacccaccg ctgcgttgat     3960

gcccaggatt tcagtccaat aatccacaat ttaccaacgg atagagctag gtgaattaga     4020

tagacaggag ggccagaggg aggggaccga gatgaaaaat tttcgatgaa agagtggtca     4080

aggtggggtc gtagttcggc gctccgaggg cgaggaacca aggaaaggcg aggaaaggac     4140

aggctgatcg cgctgcgttg ctgggctgca agcgtgtcca gttgagtctg gaaaaggctc     4200

cgccgtgaag attctgcgtt ggtcccgcac ctgcgcggtg ggggcattac ccctccatgt     4260

ccaatgattt caagtcaaag ccaagggttg aagcccgccc gcttagtcgc cttctcgctt     4320

gacccctcca tataagtatt tcccctcctc cccctcccac aaatttttcc tttccctttc     4380

ctccctcgtc cgcttcagta cgtatatctt ccccccctct ctcttccttc tcactcttct     4440

ctccttcttt cttgattcat cctctctcta actgacttct ttgctcagca cctctacgcg     4500

ttctggccgt agtatctgag caatttttct acagactttt tctatctaat tccaaaaaag     4560

aacttcgagt tcattcacca ccgtcaaaat gatctgactg atgagtccgt gaggacgaaa     4620

cgagtaagct cgtctcagat atattcagtc actggtttta gagctagaaa tagcaagtta     4680

aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttggccgg     4740

catggtccca gcctcctcgc tggcgccggc tgggcaacat gcttcggcat ggcgaatggg     4800

actaaaatgc gctaaactgg gcttgactca gggagggatc atggactagc caattgggcg     4860

tgcacagcgc gactttggag ctggttctgg ctcgcatgac ttgtttcgtg ctgcggggga     4920

ttccgttcgg acctgacatt ttaaaaataa aaaatggaaa catcttgaaa gacaaaaatg     4980

agtttcagta gtggtctaca gaccgtagtt ttgttcctat tcacagtgaa aataaggcgc     5040

tgcaattgct acgttcataa atcgagtatt gttgtgctcc gaagcgccag tccccatgtt     5100

ccgcaccctc aaagccaaag ttcgcgttcc gaccttgcct cccaaatccg agttgcgatt     5160

aatgggagac cggg                                                       5174


<210>  77
<211>  5997
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TOPO SGIC hygB

<400>  77
aagggcgaat tctgcagata tccatcacac tggcggccgc tcgagcatgc atctagaggg       60

cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt      120

gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc      180

agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagccta      240

tacgtacggc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg      300

gatgtacaga gtgatattat tgacacgccg gggcgacgga tggtgatccc cctggccagt      360

gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat      420

gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa      480

gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc      540

tggggaatat aaatgtcagg catgagatta tcaaaaagga tcttcaccta gatccttttc      600

acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct      660

atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca      720

tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg      780

gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt ctcgccgcca      840

aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc      900

atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc      960

ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca     1020

gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg     1080

caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg     1140

ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag     1200

gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg     1260

cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc     1320

atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa     1380

gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac     1440

ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat     1500

ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac     1560

atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc     1620

ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt     1680

gacgagttct tctgaattat taacgcttac aatttcctga tgcggtattt tctccttacg     1740

catctgtgcg gtatttcaca ccgcatacag gtggcacttt tcggggaaat gtgcgcggaa     1800

cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac     1860

cctgataaat gcttcaataa tagcacgtga ggagggccac catggccaag ttgaccagtg     1920

ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc     1980

tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga     2040

ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt     2100

gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc     2160

gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg     2220

ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg     2280

tgctaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca     2340

tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga     2400

tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa     2460

aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga     2520

aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt     2580

taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt     2640

taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat     2700

agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct     2760

tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca     2820

cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag     2880

agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc     2940

gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga     3000

aaaacgccag caacgcggcc tttttacggt tcctgggctt ttgctggcct tttgctcaca     3060

tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag     3120

ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg     3180

aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct     3240

ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt     3300

agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg     3360

gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc     3420

tatttaggtg acactataga atactcaagc tatgcatcaa gcttggtacc gagctcggat     3480

ccactagtaa cggccgccag tgtgctggaa ttcgcccttg ggggtctcga atggctctgt     3540

acagtgaccg gtgactcttt ctggcatgcg gagagacgga cggacgcaga gagaagggct     3600

gagtaataag cgccactgcg ccagacagct ctggcggctc tgaggtgcag tggatgatta     3660

ttaatccggg accggccgcc cctccgcccc gaagtggaaa ggctggtgtg cccctcgttg     3720

accaagaatc tattgcatca tcggagaata tggagcttca tcgaatcacc ggcagtaagc     3780

gaaggagaat gtgaagccag gggtgtatag ccgtcggcga aatagcatgc cattaaccta     3840

ggtacagaag tccaattgct tccgatctgg taaaagattc acgagatagt accttctccg     3900

aagtaggtag agcgagtacc cggcgcgtaa gctccctaat tggcccatcc ggcatctgta     3960

gggcgtccaa atatcgtgcc tctcctgctt tgcccggtgt atgaaaccgg aaaggccgct     4020

caggagctgg ccagcggcgc agaccgggaa cacaagctgg cagtcgaccc atccggtgct     4080

ctgcactcga cctgctgagg tccctcagtc cctggtaggc agctttgccc cgtctgtccg     4140

cccggtgtgt cggcggggtt gacaaggtcg ttgcgtcagt ccaacatttg ttgccatatt     4200

ttcctgctct ccccaccagc tgctcttttc ttttctcttt cttttcccat cttcagtata     4260

ttcatcttcc catccaagaa cctttatttc ccctaagtaa gtactttgct acatccatac     4320

tccatccttc ccatccctta ttcctttgaa cctttcagtt cgagctttcc cacttcatcg     4380

cagcttgact aacagctacc ccgcttgagc agacatcaca ggatcccacc gtcaaaatgc     4440

ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg     4500

acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc     4560

gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt     4620

atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca     4680

gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc     4740

ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg     4800

cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat     4860

acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa     4920

ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt     4980

gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg     5040

tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg     5100

attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc     5160

agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg     5220

cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg     5280

atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg     5340

tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag     5400

tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaataaaccc     5460

gggaggctct tcatgacgag ccaatgcatc ttttgtatgt agcttcaacc gactccgtct     5520

tcacttcttc gcccgcactg cctaccgttt gtaccatctg actcatataa atgtctagcc     5580

cctacctaca ctatacctaa gggagagaag cgtagagtga ttaacgtacg ggcctatagt     5640

accccgatct ctagatagaa catttagtag agattaggat gcctaactaa tttaacttga     5700

gcattgtccc gttcatattg attttcagtc cattatacac tcttaatcgt ttcccggtag     5760

aagcctgata tatacgacca tagggtgtgg agaacagggc ttcccgtctg cttggccgta     5820

cttaagctat atattctaca cggccaatac tcaatgtgcc cttagcacct aagcggcact     5880

ctagggtaag tgcgggtgat ataggagaga agtcttaaga ctgaagacag gatgggaaga     5940

agacggctga ccacgcaact tgcactgtcc gattctttga ctgcctcgga gaccggg        5997


<210>  78
<211>  5352
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TOPO SGIC phleo

<400>  78
aagggcgaat tctgcagata tccatcacac tggcggccgc tcgagcatgc atctagaggg       60

cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt      120

gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc      180

agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagccta      240

tacgtacggc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg      300

gatgtacaga gtgatattat tgacacgccg gggcgacgga tggtgatccc cctggccagt      360

gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat      420

gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa      480

gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc      540

tggggaatat aaatgtcagg catgagatta tcaaaaagga tcttcaccta gatccttttc      600

acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct      660

atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca      720

tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg      780

gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt ctcgccgcca      840

aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc      900

atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc      960

ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca     1020

gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg     1080

caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg     1140

ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag     1200

gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg     1260

cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc     1320

atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa     1380

gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac     1440

ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat     1500

ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac     1560

atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc     1620

ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt     1680

gacgagttct tctgaattat taacgcttac aatttcctga tgcggtattt tctccttacg     1740

catctgtgcg gtatttcaca ccgcatacag gtggcacttt tcggggaaat gtgcgcggaa     1800

cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac     1860

cctgataaat gcttcaataa tagcacgtga ggagggccac catggccaag ttgaccagtg     1920

ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc     1980

tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga     2040

ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt     2100

gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc     2160

gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg     2220

ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg     2280

tgctaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca     2340

tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga     2400

tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa     2460

aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga     2520

aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt     2580

taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt     2640

taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat     2700

agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct     2760

tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca     2820

cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag     2880

agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc     2940

gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga     3000

aaaacgccag caacgcggcc tttttacggt tcctgggctt ttgctggcct tttgctcaca     3060

tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag     3120

ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg     3180

aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct     3240

ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt     3300

agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg     3360

gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc     3420

tatttaggtg acactataga atactcaagc tatgcatcaa gcttggtacc gagctcggat     3480

ccactagtaa cggccgccag tgtgctggaa ttcgcccttg ggggtctcga atggctctgt     3540

acagtgaccg gtgactcttt ctggcatgcg gagagacgga cggacgcaga gagaagggct     3600

gagtaataag cgccactgcg ccagacagct ctggcggctc tgaggtgcag tggatgatta     3660

ttaatccggg accggccgcc cctccgcccc gaagtggaaa ggctggtgtg cccctcgttg     3720

accaagaatc tattgcatca tcggagaata tggagcttca tcgaatcacc ggcagtaagc     3780

gaaggagaat gtgaagccag gggtgtatag ccgtcggcga aatagcatgc cattaaccta     3840

ggtacagaag tccaattgct tccgatctgg taaaagattc acgagatagt accttctccg     3900

aagtaggtag agcgagtacc cggcgcgtaa gctccctaat tggcccatcc ggcatctgta     3960

gggcgtccaa atatcgtgcc tctcctgctt tgcccggtgt atgaaaccgg aaaggccgct     4020

caggagctgg ccagcggcgc agaccgggaa cacaagctgg cagtcgaccc atccggtgct     4080

ctgcactcga cctgctgagg tccctcagtc cctggtaggc agctttgccc cgtctgtccg     4140

cccggtgtgt cggcggggtt gacaaggtcg ttgcgtcagt ccaacatttg ttgccatatt     4200

ttcctgctct ccccaccagc tgctcttttc ttttctcttt cttttcccat cttcagtata     4260

ttcatcttcc catccaagaa cctttatttc ccctaagtaa gtactttgct acatccatac     4320

tccatccttc ccatccctta ttcctttgaa cctttcagtt cgagctttcc cacttcatcg     4380

cagcttgact aacagctacc ccgcttgagc agacatcaca ggatcccacc gtcaaaatgg     4440

ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt     4500

tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg     4560

tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca     4620

ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg     4680

tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt     4740

gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg     4800

agcaggacta aacccgggag gctcttcatg acgagccaat gcatcttttg tatgtagctt     4860

caaccgactc cgtcttcact tcttcgcccg cactgcctac cgtttgtacc atctgactca     4920

tataaatgtc tagcccctac ctacactata cctaagggag agaagcgtag agtgattaac     4980

gtacgggcct atagtacccc gatctctaga tagaacattt agtagagatt aggatgccta     5040

actaatttaa cttgagcatt gtcccgttca tattgatttt cagtccatta tacactctta     5100

atcgtttccc ggtagaagcc tgatatatac gaccataggg tgtggagaac agggcttccc     5160

gtctgcttgg ccgtacttaa gctatatatt ctacacggcc aatactcaat gtgcccttag     5220

cacctaagcg gcactctagg gtaagtgcgg gtgatatagg agagaagtct taagactgaa     5280

gacaggatgg gaagaagacg gctgaccacg caacttgcac tgtccgattc tttgactgcc     5340

tcggagaccg gg                                                         5352


<210>  79
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward PCR primer Cas9 with KpnI-flank

<400>  79
cccggtaccg caactctctg gaaatgaagg c                                      31


<210>  80
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse PCR primer Cas9 with KpnI-flank

<400>  80
cccggtaccg aggttcatgg tatgggcacg                                        30


<210>  81
<211>  14317
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BG-AMA8 AMA hygB / no Cas9 expression cassette

<400>  81
ggtaccttgc ccatcgaacg tacaagtact cctctgttct ctccttcctt tgctttgtgc       60

ggagaccggc ttactaaaag ccagataaca gtatgcatat ttgcgcgctg atttttgcgg      120

tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag      180

cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg      240

tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg      300

aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga      360

acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat      420

aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc      480

gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt      540

gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg      600

gccagtgtgc cggtttccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat      660

gacatcaaaa acgccattaa cctgatgttc tggggaatat aaggtctcgc ctccggatcg      720

atgtacacaa ccgactgcac ccaaacgaac acaaatctta gcagtgccct cgccggatag      780

cttggactgt cctttaccgt cgccagcaca agaagggtat ctctgaggtc cgtaccgcct      840

tttctttacc actggattcg attttcgcag ttggaatgat acatctgggg actgcgaatg      900

gtttacccct cggccgatac tatgggtcgt gaagagatgg aacattccga aagtgttttg      960

cggataacat tggtggcatc gaaaacagaa tgctgaccat tgatttcaac acgaacagga     1020

ggttgccaag aagcgtaccc gccgtgtcgt caagtcccag cgtgccatcg tcggtgcttc     1080

cctcgacgtg atcaaggagc gccgctccca gcgccccgag gcccgtgccg ccgcccgcca     1140

gcaggccatc aaggacgcca aggagaagaa ggctgccgct gagtccaaga agaaggctga     1200

gaaggctaag aacgccgctg ctggtgccaa gggtgctgct cagcgcatcc agagcaagca     1260

gggtgctaag ggttctgctc ccaaggtcgc tgccaagtct cgttaaggaa tgaataacgg     1320

ttcggcttgg gattgggtgc ggaaggcaag agtttcatgg acgaattttg ggaggttact     1380

ggagctggaa tatgtgtttt ccctaccacc aaaaatgaaa tgttccaaaa ctatcggcgt     1440

gcaagacggc ctcttacggg tttaacggct ctcagataag ctctatcaat cgcgccacgg     1500

atgcatgaat gaagatccag atggccgcgg gatatatcgt gctagtgtaa ttcctacatg     1560

atcttgctgt tcactccatg cgcatccaga tattccaggg gtcgactgtt aattgatatg     1620

cctgggcttg agactccgta gacgcccagt caatgtgcaa ttaatacgag ggtgctgtta     1680

tcggcagcaa ccttgtactt ctccataaga tgggggaatg ccatggacct gagtgatcaa     1740

ttgacgcaag tctcccataa cgcggcggct tgacctaaaa tccatatacc gccccgttga     1800

gcctccgcgc tccagagtcc tgtcccggaa tagggcacaa acctaggcta acctaattcg     1860

tcgtccgcgt ctgagttcag acaaaagaac ttccaagtat cagcagagta cgctgatatt     1920

gataagtagg caaacataag accaataagc aagtagaata aaaaattata aggacactgc     1980

ctccataaag cgccctccca agacctcagg gacaaaactt ctcaagtggc aattcactgc     2040

ctcaggccgt gtccagtgaa gtgacgaagc gacactgttg cctgctgact cagccgcttt     2100

ccgccctgcc gaatttgcca tctcgcttac aggtcagcac tagcgcgatt cgcccacaga     2160

tgctcagcgc aaagtggtga ctcagtcaaa ccccccctac aagattccac ctcgattttt     2220

caacttccca tctcgatccg acaagttcta catccaccgt caaaatggcc tccagcgaag     2280

atgtcatcaa ggagttcatg cgcttcaagg tccgcatgga aggatccgtc aacggccacg     2340

agttcgagat tgagggtgag ggtgagggcc gcccctacga aggcacccag actgccaagc     2400

tcaaggtcac caagggtggt cctctcccct tcgcttggga tatcctgtct cctcagttcc     2460

agtacggctc caaggtctac gtcaagcacc ccgccgacat ccccgactac aagaagcttt     2520

ctttccccga gggtttcaag tgggagcgtg tcatgaactt cgaggatggt ggtgttgtga     2580

ccgttactca ggacagcagc ttgcaggatg gctctttcat ctacaaggtc aagttcattg     2640

gtgtcaactt cccctccgac ggccctgtca tgcagaagaa gaccatgggc tgggaagcgt     2700

cgactgagcg tctgtacccc cgtgacggtg ttctcaaggg tgagatccac aaggctctca     2760

agctcaagga cggtggtcac taccttgttg agttcaagtc catctacatg gccaagaagc     2820

ctgtgcagct gcccggatac tactacgtgg actccaagct tgacatcacc tcccacaacg     2880

aagactacac cattgttgag cagtacgagc gtgctgaggg ccgccaccac ctcttcctga     2940

cccacggaat ggatgagctg tacaagtcga aactataaat aaatggtttg cgttgcgatt     3000

gactgaaacg aaaaaaagcg aaaatgattc tgggaatgaa ttgataaagc gcgggctctg     3060

cggtacggtt acggttgcgg tcgcggacga atggactggg ctgagctggg ctggaggaag     3120

tccatcgaac aaggacaagg ggtggaatat ggcacgggtc gattttgtta tacataccct     3180

accatccatc tatccattta aataccaaat gagttgttga atggattcgc ggtcttctcg     3240

gtttattttt gcttgcttgc gtgcttaagg gatagtgtgc ctcacgcttt ccggcatctt     3300

ccagaccaca gtatatccat ccgcctcctg ttgaagctta ttttttgtat actgttttgt     3360

gatagcacga agtttttcca cggtatcttg ttaaaaatat atatttgtgg cgggcttacc     3420

tacatcaaat taataagaga ctaattataa actaaacaca caagcaagct actttagggt     3480

aaaagtttat aaatgctttt gacgtataaa cgttgcttgt atttattatt acaattaaag     3540

gtggatagaa aacctagaga ctagttagaa actaatctca ggtttgcgtt aaactaaatc     3600

agagcccgag aggttaacag aacctagaag gggactagat atccgggtag ggaaacaaaa     3660

aaaaaaaaca agacagccac atattaggga gactagttag aagctagttc caggactagg     3720

aaaataaaag acaatgatac cacagtctag ttgacaacta gatagattct agattgaggc     3780

caaagtctct gagatccagg ttagttgcaa ctaatactag ttagtatcta gtctcctata     3840

actctgaagc tagaataact tactactatt atcctcacca ctgttcagct gcgcaaacgg     3900

agtgattgca aggtgttcag agactagtta ttgactagtc agtgactagc aataactaac     3960

aaggtattaa cctaccatgt ctgccatcac cctgcacttc ctcgggctca gcagcctttt     4020

cctcctcatt ttcatgctca ttttccttgt ttaagactgt gactagtcaa agactagtcc     4080

agaaccacaa aggagaaatg tcttaccact ttcttcattg cttgtctctt ttgcattatc     4140

catgtctgca actagttaga gtctagttag tgactagtcc gacgaggact tgcttgtctc     4200

cggattgttg gaggaactct ccagggcctc aagatccaca acagagcctt ctagaagact     4260

ggtcaataac tagttggtct ttgtctgagt ctgacttacg aggttgcata ctcgctccct     4320

ttgcctcgtc aatcgatgag aaaaagcgcc aaaactcgca atatggcttt gaaccacacg     4380

gtgctgagac tagttagaat ctagtcccaa actagcttgg atagcttacc tttgcccttt     4440

gcgttgcgac aggtcttgca gggtatggtt cctttctcac cagctgattt agctgccttg     4500

ctaccctcac ggcggatctg cataaagagt ggctagaggt tataaattag cactgatcct     4560

aggtacgggg ctgaatgtaa cttgcctttc ctttctcatc gcgcggcaag acaggcttgc     4620

tcaaattcct accagtcaca ggggtatgca cggcgtacgg accacttgaa ctagtcacag     4680

attagttagc aactagtctg cattgaatgg ctgtacttac gggccctcgc cattgtcctg     4740

atcatttcca gcttcaccct cgttgctgca aagtagttag tgactagtca aggactagtt     4800

gaaatgggag aagaaactca cgaattctcg acacccttag tattgtggtc cttggacttg     4860

gtgctgctat atattagcta atacactagt tagactcaca gaaacttacg cagctcgctt     4920

gcgcttcttg gtaggagtcg gggttgggag aacagtgcct tcaaacaagc cttcatacca     4980

tgctacttga ctagtcaggg actagtcacc aagtaatcta gataggactt gcctttggcc     5040

tccatcagtt ccttcatagt gggaggtcca ttgtgcaatg taaactccat gccgtgggag     5100

ttcttgtcct tcaagtgctt gaccaatatg tttctgttgg cagagggaac ctgtcaacta     5160

gttaataact agtcagaaac tagtatagca gtagactcac tgtacgcttg aggcatccct     5220

tcactcggca gtagacttca tatggatgga tatcaggcac gccattgtcg tcctgtggac     5280

tagtcagtaa ctaggcttaa agctagtcgg gtcggcttac tatcttgaaa tccggcagcg     5340

taagctcccc gtccttaact gcctcgagat agtgacagta ctctggggac tttcggagat     5400

cgttatcgcg aatgctcggc atactaatcg ttgactagtc ttggactagt cccgagcaaa     5460

aaggattgga ggaggaggag gaaggtgaga gtgagacaaa gagcgaaata agagcttcaa     5520

aggctatctc taagcagtat gaaggttaag tatctagttc ttgactagat ttaaaagaga     5580

tttcgactag ttatgtacct ggagtttgga tataggaatg tgttgtggta acgaaatgta     5640

agggggagga aagaaaaagt cggtcaagag gtaactctaa gtcggccatt cctttttggg     5700

aggcgctaac cataaacggc atggtcgact tagagttagc tcagggaatt tagggagtta     5760

tctgcgacca ccgaggaacg gcggaatgcc aaagaatccc gatggagctc tagctggcgg     5820

ttgacaaccc caccttttgg cgtttctgcg gcgttgcagg cgggactgga tacttcgtag     5880

aaccagaaag gcaaggcaga acgcgctcag caagagtgtt ggaagtgata gcatgatgtg     5940

ccttgttaac taggtcaaaa tctgcagtat gcttgatgtt atccaaagtg tgagagagga     6000

aggtccaaac atacacgatt gggagagggc ctaggtataa gagtttttga gtagaacgca     6060

tgtgagccca gccatctcga ggagattaaa cacgggccgg catttgatgg ctatgttagt     6120

accccaatgg aaagcctgag agtccagtgg tcgcagataa ctccctaaat tccctgagct     6180

aactctaagt cgaccatgcc gtttatggtt agcgcctccc aaaaaggaat ggccgactta     6240

gagttacctc ttgaccgact ttttctttcc tcccccttac atttcgttac cacaacacat     6300

tcctatatcc aaactccagg tacataacta gtcgaaatct cttttaaatc tagtcaagaa     6360

ctagatactt aaccttcata ctgcttagag atagcctttg aagctcttat ttcgctcttt     6420

gtctcactct caccttcctc ctcctcctcc aatccttttt gctcgggact agtccaagac     6480

tagtcaacga ttagtatgcc gagcattcgc gataacgatc tccgaaagtc cccagagtac     6540

tgtcactatc tcgaggcagt taaggacggg gagcttacgc tgccggattt caagatagta     6600

agccgacccg actagcttta agcctagtta ctgactagtc cacaggacga caatggcgtg     6660

cctgatatcc atccatatga agtctactgc cgagtgaagg gatgcctcaa gcgtacagtg     6720

agtctactgc tatactagtt tctgactagt tattaactag ttgacaggtt ccctctgcca     6780

acagaaacat attggtcaag cacttgaagg acaagaactc ccacggcatg gagtttacat     6840

tgcacaatgg acctcccact atgaaggaac tgatggaggc caaaggcaag tcctatctag     6900

attacttggt gactagtccc tgactagtca agtagcatgg tatgaaggct tgtttgaagg     6960

cactgttctc ccaaccccga ctcctaccaa gaagcgcaag cgagctgcgt aagtttctgt     7020

gagtctaact agtgtattag ctaatatata gcagcaccaa gtccaaggac cacaatacta     7080

agggtgtcga gaattcgtga gtttcttctc ccatttcaac tagtccttga ctagtcacta     7140

actactttgc agcaacgagg gtgaagctgg aaatgatcag gacaatggcg agggcccgta     7200

agtacagcca ttcaatgcag actagttgct aactaatctg tgactagttc aagtggtccg     7260

tacgccgtgc atacccctgt gactggtagg aatttgagca agcctgtctt gccgcgcgat     7320

gagaaaggaa aggcaagtta cattcagccc cgtacctagg atcagtgcta atttataacc     7380

tctagccact ctttatgcag atccgccgtg agggtagcaa ggcagctaaa tcagctggtg     7440

agaaaggaac cataccctgc aagacctgtc gcaacgcaaa gggcaaaggt aagctatcca     7500

agctagtttg ggactagatt ctaactagtc tcagcaccgt gtggttcaaa gccatattgc     7560

gagttttggc gctttttctc atcgattgac gaggcaaagg gagcgagtat gcaacctcgt     7620

aagtcagact cagacaaaga ccaactagtt attgaccagt cttctagaag gctctgttgt     7680

ggatcttgag gccctggaga gttcctccaa caatccggag acaagcaagt cctcgtcgga     7740

ctagtcacta actagactct aactagttgc agacatggat aatgcaaaag agacaagcaa     7800

tgaagaaagt ggtaagacat ttctcctttg tggttctgga ctagtctttg actagtcaca     7860

gtcttaaaca aggaaaatga gcatgaaaat gaggaggaaa aggctgctga gcccgaggaa     7920

gtgcagggtg atggcagaca tggtaggtta ataccttgtt agttattgct agtcactgac     7980

tagtcaataa ctagtctctg aacaccttgc aatcactccg tttgcgcagc tgaacagtgg     8040

tgaggataat agtagtaagt tattctagct tcagagttat aggagactag atactaacta     8100

gtattagttg caactaacct ggatctcaga gactttggcc tcaatctaga atctatctag     8160

ttgtcaacta gactgtggta tcattgtctt ttattttcct agtcctggaa ctagcttcta     8220

actagtctcc ctaatatgtg gctgtcttgt tttttttttt tgtttcccta cccggatatc     8280

tagtcccctt ctaggttctg ttaacctctc gggctctgat ttagtttaac gcaaacctga     8340

gattagtttc taactagtct ctaggttttc tatccacctt taattgtaat aataaataca     8400

agcaacgttt atacgtcaaa agcatttata aacttttacc ctaaagtagc ttgcttgtgt     8460

gtttagttta taattagtct cttattaatt tgatgtaggt aagcccgcca caaatatata     8520

tttttaacaa gataccgtgg aaaaacttcg tgctatcaca aaacagtata caaaaaataa     8580

gctatcgaat tcctgcagag atcatcctgt cttcagtctt aagacttctc tcctatatca     8640

cccgcactta ccctagagtg ccgcttaggt gctaagggca cattgagtat tggccgtgta     8700

gaatatatag cttaagtacg gccaagcaga cgggaagccc tgttctccac accctatggt     8760

cgtatatatc aggcttctac cgggaaacga ttaagagtgt ataatggact gaaaatcaat     8820

atgaacggga caatgctcaa gttaaattag ttaggcatcc taatctctac taaatgttct     8880

atctagagat cggggtacta taggcccgta cgttaatcac tctacgcttc tctcccttag     8940

gtatagtgta ggtaggggct agacatttat atgagtcaga tggtacaaac ggtaggcagt     9000

gcgggcgaag aagtgaagac ggagtcggtt gaagctacat acaaaagatg cattggctcg     9060

tcatgaagag cctcccgggt ttattccttt gccctcggac gagtgctggg gcgtcggttt     9120

ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg     9180

atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc     9240

gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca     9300

atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg cctccgctcg     9360

aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg     9420

acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg     9480

ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg ccggacttcg     9540

gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga cgcactgacg     9600

gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc gcatatgaaa     9660

tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa cccgctcgtc     9720

tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg cagaacagcg     9780

ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa     9840

taggtcaggc tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc     9900

gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca gctatttacc     9960

cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc ttcgccctcc    10020

gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt ctcgacagac    10080

gtcgcggtga gttcaggcat tttgacggtg ggatcctgtg atgtctgctc aagcggggta    10140

gctgttagtc aagctgcgat gaagtgggaa agctcgaact gaaaggttca aaggaataag    10200

ggatgggaag gatggagtat ggatgtagca aagtacttac ttaggggaaa taaaggttct    10260

tggatgggaa gatgaatata ctgaagatgg gaaaagaaag agaaaagaaa agagcagctg    10320

gtggggagag caggaaaata tggcaacaaa tgttggactg acgcaacgac cttgtcaacc    10380

ccgccgacac accgggcgga cagacggggc aaagctgcct accagggact gagggacctc    10440

agcaggtcga gtgcagagca ccggatgggt cgactgccag cttgtgttcc cggtctgcgc    10500

cgctggccag ctcctgagcg gcctttccgg tttcatacac cgggcaaagc aggagaggca    10560

cgatatttgg acgccctaca gatgccggat gggccaatta gggagcttac gcgccgggta    10620

ctcgctctac ctacttcgga gaaggtacta tctcgtgaat cttttaccag atcggaagca    10680

attggacttc tgtacctagg ttaatggcat gctatttcgc cgacggctat acacccctgg    10740

cttcacattc tccttcgctt actgccggtg attcgatgaa gctccatatt ctccgatgat    10800

gcaatagatt cttggtcaac gaggggcaca ccagcctttc cacttcgggg cggaggggcg    10860

gccggtcccg gattaataat catccactgc acctcagagc cgccagagct gtctggcgca    10920

gtggcgctta ttactcagcc cttctctctg cgtccgtccg tctctccgca tgccagaaag    10980

agtcaccggt cactgtacag agcggccgcc accgcggtgg agctccaatt cgccctatag    11040

tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc    11100

tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag    11160

cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga    11220

cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc    11280

tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac    11340

gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag    11400

tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc    11460

atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg    11520

actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata    11580

agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa    11640

cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg    11700

cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga    11760

caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat    11820

ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca    11880

gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc    11940

gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca    12000

atgatgagca cttttcgacc gaataaatac ctgtgacgga agatcacttc gcagaataaa    12060

taaatcctgg tgtccctgtt gataccggga agccctgggc caacttttgg cgaaaatgag    12120

acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg    12180

gcgtattttt tgagttgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa    12240

tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat    12300

ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt    12360

taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc    12420

gcctgatgaa tgctcatccg gaattacgta tggcaatgaa agacggtgag ctggtgatat    12480

gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc    12540

tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg    12600

cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg    12660

tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca    12720

acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga    12780

tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc    12840

ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca    12900

gttattggtg cccttaaacg cctggttgct acgcctgaat aagtgataat aagcggatga    12960

atggcagaaa ttcgaaagca aattcgaccc ggtcgtcggt tcagggcagg gtcgttaaat    13020

agccgcttat gtctattgct ggtttaccgg tttattgact accggaagca gtgtgaccgt    13080

gtgcttctca aatgcctgag gccagtttgc tcaggctctc cccgtggagg taataattga    13140

cgatatgatc ctttttttct gatcaaaaag gatctaggtg aagatccttt ttgataatct    13200

catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa    13260

gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa    13320

aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc    13380

gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta    13440

gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct    13500

gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg    13560

atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag    13620

cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc    13680

cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg    13740

agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt    13800

tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg    13860

gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca    13920

catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg    13980

agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc    14040

ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag    14100

ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag    14160

ttagctcact cattaggcac cccaggcttt acactttatg ctcccggctc gtatgttgtg    14220

tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa    14280

gcgcgcaatt aaccctcact aaagggaaca aaagctg                             14317


<210>  82
<211>  19242
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BG-AMA14 AMA phleo/Cas9 ++.

<400>  82
cttgcccatc gaacgtacaa gtactcctct gttctctcct tcctttgctt tgtgcggaga       60

ccggcttact aaaagccaga taacagtatg catatttgcg cgctgatttt tgcggtataa      120

gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg      180

tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat      240

atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc      300

tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc      360

tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag      420

agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg      480

acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact      540

ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag      600

tgtgccggtt tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat      660

caaaaacgcc attaacctga tgttctgggg aatataaggt ctcgcctccg gatcgatgta      720

cacaaccgac tgcacccaaa cgaacacaaa tcttagcagt gccctcgccg gatagcttgg      780

actgtccttt accgtcgcca gcacaagaag ggtatctctg aggtccgtac cgccttttct      840

ttaccactgg attcgatttt cgcagttgga atgatacatc tggggactgc gaatggttta      900

cccctcggcc gatactatgg gtcgtgaaga gatggaacat tccgaaagtg ttttgcggat      960

aacattggtg gcatcgaaaa cagaatgctg accattgatt tcaacacgaa caggaggttg     1020

ccaagaagcg tacccgccgt gtcgtcaagt cccagcgtgc catcgtcggt gcttccctcg     1080

acgtgatcaa ggagcgccgc tcccagcgcc ccgaggcccg tgccgccgcc cgccagcagg     1140

ccatcaagga cgccaaggag aagaaggctg ccgctgagtc caagaagaag gctgagaagg     1200

ctaagaacgc cgctgctggt gccaagggtg ctgctcagcg catccagagc aagcagggtg     1260

ctaagggttc tgctcccaag gtcgctgcca agtctcgtta aggaatgaat aacggttcgg     1320

cttgggattg ggtgcggaag gcaagagttt catggacgaa ttttgggagg ttactggagc     1380

tggaatatgt gttttcccta ccaccaaaaa tgaaatgttc caaaactatc ggcgtgcaag     1440

acggcctctt acgggtttaa cggctctcag ataagctcta tcaatcgcgc cacggatgca     1500

tgaatgaaga tccagatggc cgcgggatat atcgtgctag tgtaattcct acatgatctt     1560

gctgttcact ccatgcgcat ccagatattc caggggtcga ctgttaattg atatgcctgg     1620

gcttgagact ccgtagacgc ccagtcaatg tgcaattaat acgagggtgc tgttatcggc     1680

agcaaccttg tacttctcca taagatgggg gaatgccatg gacctgagtg atcaattgac     1740

gcaagtctcc cataacgcgg cggcttgacc taaaatccat ataccgcccc gttgagcctc     1800

cgcgctccag agtcctgtcc cggaataggg cacaaaccta ggctaaccta attcgtcgtc     1860

cgcgtctgag ttcagacaaa agaacttcca agtatcagca gagtacgctg atattgataa     1920

gtaggcaaac ataagaccaa taagcaagta gaataaaaaa ttataaggac actgcctcca     1980

taaagcgccc tcccaagacc tcagggacaa aacttctcaa gtggcaattc actgcctcag     2040

gccgtgtcca gtgaagtgac gaagcgacac tgttgcctgc tgactcagcc gctttccgcc     2100

ctgccgaatt tgccatctcg cttacaggtc agcactagcg cgattcgccc acagatgctc     2160

agcgcaaagt ggtgactcag tcaaaccccc cctacaagat tccacctcga tttttcaact     2220

tcccatctcg atccgacaag ttctacatcc accgtcaaaa tggcctccag cgaagatgtc     2280

atcaaggagt tcatgcgctt caaggtccgc atggaaggat ccgtcaacgg ccacgagttc     2340

gagattgagg gtgagggtga gggccgcccc tacgaaggca cccagactgc caagctcaag     2400

gtcaccaagg gtggtcctct ccccttcgct tgggatatcc tgtctcctca gttccagtac     2460

ggctccaagg tctacgtcaa gcaccccgcc gacatccccg actacaagaa gctttctttc     2520

cccgagggtt tcaagtggga gcgtgtcatg aacttcgagg atggtggtgt tgtgaccgtt     2580

actcaggaca gcagcttgca ggatggctct ttcatctaca aggtcaagtt cattggtgtc     2640

aacttcccct ccgacggccc tgtcatgcag aagaagacca tgggctggga agcgtcgact     2700

gagcgtctgt acccccgtga cggtgttctc aagggtgaga tccacaaggc tctcaagctc     2760

aaggacggtg gtcactacct tgttgagttc aagtccatct acatggccaa gaagcctgtg     2820

cagctgcccg gatactacta cgtggactcc aagcttgaca tcacctccca caacgaagac     2880

tacaccattg ttgagcagta cgagcgtgct gagggccgcc accacctctt cctgacccac     2940

ggaatggatg agctgtacaa gtcgaaacta taaataaatg gtttgcgttg cgattgactg     3000

aaacgaaaaa aagcgaaaat gattctggga atgaattgat aaagcgcggg ctctgcggta     3060

cggttacggt tgcggtcgcg gacgaatgga ctgggctgag ctgggctgga ggaagtccat     3120

cgaacaagga caaggggtgg aatatggcac gggtcgattt tgttatacat accctaccat     3180

ccatctatcc atttaaatac caaatgagtt gttgaatgga ttcgcggtct tctcggttta     3240

tttttgcttg cttgcgtgct taagggatag tgtgcctcac gctttccggc atcttccaga     3300

ccacagtata tccatccgcc tcctgttgaa gcttattttt tgtatactgt tttgtgatag     3360

cacgaagttt ttccacggta tcttgttaaa aatatatatt tgtggcgggc ttacctacat     3420

caaattaata agagactaat tataaactaa acacacaagc aagctacttt agggtaaaag     3480

tttataaatg cttttgacgt ataaacgttg cttgtattta ttattacaat taaaggtgga     3540

tagaaaacct agagactagt tagaaactaa tctcaggttt gcgttaaact aaatcagagc     3600

ccgagaggtt aacagaacct agaaggggac tagatatccg ggtagggaaa caaaaaaaaa     3660

aaacaagaca gccacatatt agggagacta gttagaagct agttccagga ctaggaaaat     3720

aaaagacaat gataccacag tctagttgac aactagatag attctagatt gaggccaaag     3780

tctctgagat ccaggttagt tgcaactaat actagttagt atctagtctc ctataactct     3840

gaagctagaa taacttacta ctattatcct caccactgtt cagctgcgca aacggagtga     3900

ttgcaaggtg ttcagagact agttattgac tagtcagtga ctagcaataa ctaacaaggt     3960

attaacctac catgtctgcc atcaccctgc acttcctcgg gctcagcagc cttttcctcc     4020

tcattttcat gctcattttc cttgtttaag actgtgacta gtcaaagact agtccagaac     4080

cacaaaggag aaatgtctta ccactttctt cattgcttgt ctcttttgca ttatccatgt     4140

ctgcaactag ttagagtcta gttagtgact agtccgacga ggacttgctt gtctccggat     4200

tgttggagga actctccagg gcctcaagat ccacaacaga gccttctaga agactggtca     4260

ataactagtt ggtctttgtc tgagtctgac ttacgaggtt gcatactcgc tccctttgcc     4320

tcgtcaatcg atgagaaaaa gcgccaaaac tcgcaatatg gctttgaacc acacggtgct     4380

gagactagtt agaatctagt cccaaactag cttggatagc ttacctttgc cctttgcgtt     4440

gcgacaggtc ttgcagggta tggttccttt ctcaccagct gatttagctg ccttgctacc     4500

ctcacggcgg atctgcataa agagtggcta gaggttataa attagcactg atcctaggta     4560

cggggctgaa tgtaacttgc ctttcctttc tcatcgcgcg gcaagacagg cttgctcaaa     4620

ttcctaccag tcacaggggt atgcacggcg tacggaccac ttgaactagt cacagattag     4680

ttagcaacta gtctgcattg aatggctgta cttacgggcc ctcgccattg tcctgatcat     4740

ttccagcttc accctcgttg ctgcaaagta gttagtgact agtcaaggac tagttgaaat     4800

gggagaagaa actcacgaat tctcgacacc cttagtattg tggtccttgg acttggtgct     4860

gctatatatt agctaataca ctagttagac tcacagaaac ttacgcagct cgcttgcgct     4920

tcttggtagg agtcggggtt gggagaacag tgccttcaaa caagccttca taccatgcta     4980

cttgactagt cagggactag tcaccaagta atctagatag gacttgcctt tggcctccat     5040

cagttccttc atagtgggag gtccattgtg caatgtaaac tccatgccgt gggagttctt     5100

gtccttcaag tgcttgacca atatgtttct gttggcagag ggaacctgtc aactagttaa     5160

taactagtca gaaactagta tagcagtaga ctcactgtac gcttgaggca tcccttcact     5220

cggcagtaga cttcatatgg atggatatca ggcacgccat tgtcgtcctg tggactagtc     5280

agtaactagg cttaaagcta gtcgggtcgg cttactatct tgaaatccgg cagcgtaagc     5340

tccccgtcct taactgcctc gagatagtga cagtactctg gggactttcg gagatcgtta     5400

tcgcgaatgc tcggcatact aatcgttgac tagtcttgga ctagtcccga gcaaaaagga     5460

ttggaggagg aggaggaagg tgagagtgag acaaagagcg aaataagagc ttcaaaggct     5520

atctctaagc agtatgaagg ttaagtatct agttcttgac tagatttaaa agagatttcg     5580

actagttatg tacctggagt ttggatatag gaatgtgttg tggtaacgaa atgtaagggg     5640

gaggaaagaa aaagtcggtc aagaggtaac tctaagtcgg ccattccttt ttgggaggcg     5700

ctaaccataa acggcatggt cgacttagag ttagctcagg gaatttaggg agttatctgc     5760

gaccaccgag gaacggcgga atgccaaaga atcccgatgg agctctagct ggcggttgac     5820

aaccccacct tttggcgttt ctgcggcgtt gcaggcggga ctggatactt cgtagaacca     5880

gaaaggcaag gcagaacgcg ctcagcaaga gtgttggaag tgatagcatg atgtgccttg     5940

ttaactaggt caaaatctgc agtatgcttg atgttatcca aagtgtgaga gaggaaggtc     6000

caaacataca cgattgggag agggcctagg tataagagtt tttgagtaga acgcatgtga     6060

gcccagccat ctcgaggaga ttaaacacgg gccggcattt gatggctatg ttagtacccc     6120

aatggaaagc ctgagagtcc agtggtcgca gataactccc taaattccct gagctaactc     6180

taagtcgacc atgccgttta tggttagcgc ctcccaaaaa ggaatggccg acttagagtt     6240

acctcttgac cgactttttc tttcctcccc cttacatttc gttaccacaa cacattccta     6300

tatccaaact ccaggtacat aactagtcga aatctctttt aaatctagtc aagaactaga     6360

tacttaacct tcatactgct tagagatagc ctttgaagct cttatttcgc tctttgtctc     6420

actctcacct tcctcctcct cctccaatcc tttttgctcg ggactagtcc aagactagtc     6480

aacgattagt atgccgagca ttcgcgataa cgatctccga aagtccccag agtactgtca     6540

ctatctcgag gcagttaagg acggggagct tacgctgccg gatttcaaga tagtaagccg     6600

acccgactag ctttaagcct agttactgac tagtccacag gacgacaatg gcgtgcctga     6660

tatccatcca tatgaagtct actgccgagt gaagggatgc ctcaagcgta cagtgagtct     6720

actgctatac tagtttctga ctagttatta actagttgac aggttccctc tgccaacaga     6780

aacatattgg tcaagcactt gaaggacaag aactcccacg gcatggagtt tacattgcac     6840

aatggacctc ccactatgaa ggaactgatg gaggccaaag gcaagtccta tctagattac     6900

ttggtgacta gtccctgact agtcaagtag catggtatga aggcttgttt gaaggcactg     6960

ttctcccaac cccgactcct accaagaagc gcaagcgagc tgcgtaagtt tctgtgagtc     7020

taactagtgt attagctaat atatagcagc accaagtcca aggaccacaa tactaagggt     7080

gtcgagaatt cgtgagtttc ttctcccatt tcaactagtc cttgactagt cactaactac     7140

tttgcagcaa cgagggtgaa gctggaaatg atcaggacaa tggcgagggc ccgtaagtac     7200

agccattcaa tgcagactag ttgctaacta atctgtgact agttcaagtg gtccgtacgc     7260

cgtgcatacc cctgtgactg gtaggaattt gagcaagcct gtcttgccgc gcgatgagaa     7320

aggaaaggca agttacattc agccccgtac ctaggatcag tgctaattta taacctctag     7380

ccactcttta tgcagatccg ccgtgagggt agcaaggcag ctaaatcagc tggtgagaaa     7440

ggaaccatac cctgcaagac ctgtcgcaac gcaaagggca aaggtaagct atccaagcta     7500

gtttgggact agattctaac tagtctcagc accgtgtggt tcaaagccat attgcgagtt     7560

ttggcgcttt ttctcatcga ttgacgaggc aaagggagcg agtatgcaac ctcgtaagtc     7620

agactcagac aaagaccaac tagttattga ccagtcttct agaaggctct gttgtggatc     7680

ttgaggccct ggagagttcc tccaacaatc cggagacaag caagtcctcg tcggactagt     7740

cactaactag actctaacta gttgcagaca tggataatgc aaaagagaca agcaatgaag     7800

aaagtggtaa gacatttctc ctttgtggtt ctggactagt ctttgactag tcacagtctt     7860

aaacaaggaa aatgagcatg aaaatgagga ggaaaaggct gctgagcccg aggaagtgca     7920

gggtgatggc agacatggta ggttaatacc ttgttagtta ttgctagtca ctgactagtc     7980

aataactagt ctctgaacac cttgcaatca ctccgtttgc gcagctgaac agtggtgagg     8040

ataatagtag taagttattc tagcttcaga gttataggag actagatact aactagtatt     8100

agttgcaact aacctggatc tcagagactt tggcctcaat ctagaatcta tctagttgtc     8160

aactagactg tggtatcatt gtcttttatt ttcctagtcc tggaactagc ttctaactag     8220

tctccctaat atgtggctgt cttgtttttt ttttttgttt ccctacccgg atatctagtc     8280

cccttctagg ttctgttaac ctctcgggct ctgatttagt ttaacgcaaa cctgagatta     8340

gtttctaact agtctctagg ttttctatcc acctttaatt gtaataataa atacaagcaa     8400

cgtttatacg tcaaaagcat ttataaactt ttaccctaaa gtagcttgct tgtgtgttta     8460

gtttataatt agtctcttat taatttgatg taggtaagcc cgccacaaat atatattttt     8520

aacaagatac cgtggaaaaa cttcgtgcta tcacaaaaca gtatacaaaa aataagctat     8580

cgaattcctg cagagatcat cctgtcttca gtcttaagac ttctctccta tatcacccgc     8640

acttacccta gagtgccgct taggtgctaa gggcacattg agtattggcc gtgtagaata     8700

tatagcttaa gtacggccaa gcagacggga agccctgttc tccacaccct atggtcgtat     8760

atatcaggct tctaccggga aacgattaag agtgtataat ggactgaaaa tcaatatgaa     8820

cgggacaatg ctcaagttaa attagttagg catcctaatc tctactaaat gttctatcta     8880

gagatcgggg tactataggc ccgtacgtta atcactctac gcttctctcc cttaggtata     8940

gtgtaggtag gggctagaca tttatatgag tcagatggta caaacggtag gcagtgcggg     9000

cgaagaagtg aagacggagt cggttgaagc tacatacaaa agatgcattg gctcgtcatg     9060

aagagcctcc cgggtttagt cctgctcctc ggccacgaag tgcacgcagt tgccggccgg     9120

gtcgcgcagg gcgaactccc gcccccacgg ctgctcgccg atctcggtca tggccggccc     9180

ggaggcgtcc cggaagttcg tggacacgac ctccgaccac tcggcgtaca gctcgtccag     9240

gccgcgcacc cacacccagg ccagggtgtt gtccggcacc acctggtcct ggaccgcgct     9300

gatgaacagg gtcacgtcgt cccggaccac accggcgaag tcgtcctcca cgaagtcccg     9360

ggagaacccg agccggtcgg tccagaactc gaccgctccg gcgacgtcgc gcgcggtgag     9420

caccggaacg gcactggtca acttggccat tttgacggtg ggatcctgtg atgtctgctc     9480

aagcggggta gctgttagtc aagctgcgat gaagtgggaa agctcgaact gaaaggttca     9540

aaggaataag ggatgggaag gatggagtat ggatgtagca aagtacttac ttaggggaaa     9600

taaaggttct tggatgggaa gatgaatata ctgaagatgg gaaaagaaag agaaaagaaa     9660

agagcagctg gtggggagag caggaaaata tggcaacaaa tgttggactg acgcaacgac     9720

cttgtcaacc ccgccgacac accgggcgga cagacggggc aaagctgcct accagggact     9780

gagggacctc agcaggtcga gtgcagagca ccggatgggt cgactgccag cttgtgttcc     9840

cggtctgcgc cgctggccag ctcctgagcg gcctttccgg tttcatacac cgggcaaagc     9900

aggagaggca cgatatttgg acgccctaca gatgccggat gggccaatta gggagcttac     9960

gcgccgggta ctcgctctac ctacttcgga gaaggtacta tctcgtgaat cttttaccag    10020

atcggaagca attggacttc tgtacctagg ttaatggcat gctatttcgc cgacggctat    10080

acacccctgg cttcacattc tccttcgctt actgccggtg attcgatgaa gctccatatt    10140

ctccgatgat gcaatagatt cttggtcaac gaggggcaca ccagcctttc cacttcgggg    10200

cggaggggcg gccggtcccg gattaataat catccactgc acctcagagc cgccagagct    10260

gtctggcgca gtggcgctta ttactcagcc cttctctctg cgtccgtccg tctctccgca    10320

tgccagaaag agtcaccggt cactgtacag agcggccgcc accgcggtgg agctccaatt    10380

cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact    10440

gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct    10500

ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg    10560

gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca    10620

gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct    10680

ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt    10740

tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac    10800

gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct    10860

ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt    10920

ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac    10980

aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg    11040

gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc    11100

gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag    11160

tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt    11220

tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt    11280

gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga    11340

acgttttcca atgatgagca cttttcgacc gaataaatac ctgtgacgga agatcacttc    11400

gcagaataaa taaatcctgg tgtccctgtt gataccggga agccctgggc caacttttgg    11460

cgaaaatgag acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga    11520

tcactaccgg gcgtattttt tgagttgtcg agattttcag gagctaagga agctaaaatg    11580

gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat    11640

tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt    11700

acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac    11760

attcttgccc gcctgatgaa tgctcatccg gaattacgta tggcaatgaa agacggtgag    11820

ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg    11880

ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg    11940

caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat    12000

atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc    12060

aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac    12120

aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc    12180

ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt    12240

ttttaaggca gttattggtg cccttaaacg cctggttgct acgcctgaat aagtgataat    12300

aagcggatga atggcagaaa ttcgaaagca aattcgaccc ggtcgtcggt tcagggcagg    12360

gtcgttaaat agccgcttat gtctattgct ggtttaccgg tttattgact accggaagca    12420

gtgtgaccgt gtgcttctca aatgcctgag gccagtttgc tcaggctctc cccgtggagg    12480

taataattga cgatatgatc ctttttttct gatcaaaaag gatctaggtg aagatccttt    12540

ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc    12600

ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct    12660

tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa    12720

ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag    12780

tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc    12840

tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg    12900

actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca    12960

cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat    13020

gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg    13080

tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc    13140

ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc    13200

ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc    13260

cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg    13320

cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga    13380

gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc    13440

attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa    13500

ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg ctcccggctc    13560

gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg    13620

attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctgggt acgcttgcac    13680

caatcgccgt ttaggtgttc attaggttgt attttggcta tttattgcga tatttaagcg    13740

aggcgaacat gggataaaac gtttgctgtt agtgtttgtg tatatgctag cgacttagag    13800

tgcacgctca aaagataccc ctaacttctg atttgatagc atttgtatat ggcatatagt    13860

acgcctgaag cataaaaaaa aaaacagaat tattatagtt tattaatacg agacagcaga    13920

atcaccgccc aagttaagcc tttgtgctga tcatgctctc gaacgggcca agttcgggaa    13980

aagcaaagga gcgtttagtg aggggcaatt tgactcacct cccaggcaac agatgagggg    14040

ggcaaaaaga aagaaatttt cgtgagtcaa tatggattcc gagcatcatt ttcttgcggt    14100

ctatcttgct acgtatgttg atcttgacgc tgtggatcaa gcaacgccac tcgctcgctc    14160

catcgcaggc tggtcgcaga caaattaaaa ggcggcaaac tcgtacagcc gcggggttgt    14220

ccgctgcaaa gtacagagtg ataaaagccg ccatgcgacc atcaacgcgt tgatgcccag    14280

ctttttcgat ccgagaatcc accgtagagg cgatagcaag taaagaaaag ctaaacaaaa    14340

aaaaatttct gcccctaagc catgaaaacg agatggggtg gagcagaacc aaggaaagag    14400

tcgcgctggg ctgccgttcc ggaaggtgtt gtaaaggctc gacgcccaag gtgggagtct    14460

aggagaagaa tttgcatcgg gagtggggcg ggttacccct ccatatccaa tgacagatat    14520

ctaccagcca agggtttgag cccgcccgct tagtcgtcgt cctcgcttgc ccctccataa    14580

aaggatttcc cctccccctc ccacaaaatt ttctttccct tcctctcctt gtccgcttca    14640

gtacgtatat cttcccttcc ctcgcttctc tcctccatcc ttctttcatc catctcctgc    14700

taacttctct gctcagcacc tctacgcatt actagccgta gtatctgagc acttctccct    14760

tttatattcc acaaaacata acaccaccgt caaaatggac aagaagtata gcatcggtct    14820

ggacattggc accaactccg ttggctgggc tgtcatcacc gacgagtaca aggttcctag    14880

caagaaattc aaggtcctgg gaaacaccga tcgtcactcc atcaagaaga acctcattgg    14940

tgcgcttctc ttcgactccg gtgagactgc tgaggccacc cgtctgaagc gtaccgctcg    15000

ccgtcgttac actcgccgca agaaccgtat ctgctacctc caggagattt tctccaacga    15060

gatggccaag gttgatgact ctttcttcca ccgtctggag gagtcgttcc ttgttgaaga    15120

agacaagaag cacgagcgtc accctatctt cggtaacatt gtcgatgagg tcgcttacca    15180

cgagaagtac cccaccatct accacctacg caaaaagctc gtcgacagca ccgacaaggc    15240

tgacctccgc ctcatttacc tggctctggc gcacatgatc aagttccgtg gtcacttcct    15300

gatcgagggt gacctcaacc ccgacaactc cgatgttgat aaactcttca tccagctcgt    15360

tcagacctac aaccagcttt tcgaggaaaa ccccatcaac gcgtctggcg tggatgccaa    15420

ggccatcctc tccgctcgcc tgagcaagtc ccgccgtctt gagaacttga ttgcccagct    15480

ccctggtgag aagaagaacg gtcttttcgg caaccttatt gccctgtccc tcggactgac    15540

tcccaacttc aagagcaact tcgatcttgc tgaggatgct aagttacaac tttccaagga    15600

tacctacgac gacgaccttg ataaccttct cgcccagata ggagatcagt acgccgacct    15660

cttcctagct gccaagaacc tctccgatgc cattctcctg tcagatatcc tccgtgtcaa    15720

cactgagatc accaaggccc ctctctccgc ctctatgatc aagagatacg atgagcacca    15780

ccaggacctc accctactca aggctctggt ccgccagcag ctccccgaaa agtacaagga    15840

gatcttcttc gaccagtcca agaacggcta cgccggttac atcgacggtg gtgcttccca    15900

ggaagaattc tacaagttca ttaagcctat cctcgagaag atggatggca ctgaggagct    15960

tcttgttaag ctgaaccgtg aggaccttct gcgcaagcag cgtactttcg acaacggcag    16020

catcccccac cagatccacc tgggtgaatt gcacgccatc cttcgtcgcc aggaagactt    16080

ctaccctttc ttgaaggaca accgtgagaa gattgagaag atcctgacct tccgtatccc    16140

ctactacgtc ggtcctctgg ctcgcggtaa ctcccgcttc gcctggatga cccgcaagtc    16200

cgaggaaacc atcaccccct ggaacttcga ggaagtcgtc gacaagggtg cctccgctca    16260

gagcttcatt gagcgtatga ccaacttcga caagaacctg cccaacgaga aagtcctgcc    16320

caagcactcc ctcttgtacg agtacttcac tgtctacaac gagctgacca aggtcaagta    16380

cgtgaccgag ggcatgcgca agcctgcttt cctctccggc gaacagaaga aggccattgt    16440

cgacctgctg ttcaagacta accgcaaggt gaccgtcaag cagctcaagg aagactactt    16500

caagaagatc gagtgctttg actccgttga gatctccggt gttgaggacc gcttcaacgc    16560

ttctctcggc acctaccacg atctgctcaa gatcatcaag gacaaggact tccttgacaa    16620

cgaggagaac gaagacattc ttgaggacat tgttcttacc ctcaccctct tcgaggaccg    16680

tgagatgatc gaagaacgtc tgaagaccta cgctcacctc ttcgacgaca aggtcatgaa    16740

gcagttgaag cgccgccgtt acactggctg gggtcgcctc tctcgcaagt tgattaacgg    16800

tatccgtgat aagcagtctg gcaagaccat ccttgacttc ctgaagtccg acggcttcgc    16860

caaccgcaac ttcatgcagc tcatccacga cgactctctg accttcaaag aggacatcca    16920

gaaggcccaa gtctccggcc agggtgactc gctacacgaa cacattgcca acctggctgg    16980

ttcccccgct atcaagaagg gtatcctgca gactgtgaag gttgttgacg agcttgtgaa    17040

ggtcatgggt cgtcacaagc ccgagaacat cgtcatcgaa atggctcgtg agaaccagac    17100

cactcagaag ggtcagaaga acagccgtga gcgcatgaag cgtatcgagg aaggcatcaa    17160

ggagctcggt tcccagattc tcaaggaaca ccccgtcgag aacacccagc tgcagaatga    17220

gaagctctac ctctactact tgcagaacgg acgtgacatg tacgtcgacc aggagctgga    17280

tatcaaccgc ctctccgact acgatgttga ccacatcgtc ccccagtcct tcctcaagga    17340

tgacagcatt gacaacaagg tgctcacccg ttccgacaag aatcgtggca agagcgataa    17400

cgtcccctcg gaagaggttg ttaagaagat gaagaactac tggagacaat tgctcaacgc    17460

taagctcatc actcagcgca agttcgacaa ccttaccaag gccgagcgtg gcggactctc    17520

cgagctcgac aaggccggtt tcatcaagcg tcaattggtt gaaacccgtc agatcactaa    17580

gcacgttgcc cagatcctgg actctcgcat gaacaccaag tacgacgaga acgacaagct    17640

catccgtgag gtcaaggtca tcaccttaaa gagcaagctg gtcagtgact ttaggaaaga    17700

cttccagttc tacaaggtcc gcgagatcaa caactaccac cacgctcacg atgcctacct    17760

caacgccgtc gtcggtactg ctttgattaa gaagtatccc aagctcgagt ccgagttcgt    17820

ctacggtgac tacaaggtgt acgacgtgcg caagatgatc gctaagtccg agcaggagat    17880

cggaaaggcc actgccaagt acttcttcta cagcaacatc atgaacttct tcaagaccga    17940

aataacattg gccaacggcg agattcgcaa gcgtcccttg attgagacta acggcgaaac    18000

cggtgagatc gtctgggaca agggccgtga cttcgctacc gtccgcaagg tcctttctat    18060

gccccaggtc aacattgtca agaagaccga ggtgcagact ggtggtttct ccaaggagtc    18120

gattcttccc aagcgcaact ccgacaagct gatcgctcgc aagaaggatt gggaccccaa    18180

gaagtacggt ggattcgatt cgcctaccgt tgcctactcc gtcttggttg tcgccaaggt    18240

cgagaagggc aagagcaaga agctgaagag tgtgaaggaa ctcctcggta tcaccatcat    18300

ggaacgcagc agcttcgaga agaaccctat cgacttcctg gaggccaagg gttacaaaga    18360

ggtcaagaag gacctcatca tcaagctccc caagtactct ctgttcgagc tggagaacgg    18420

ccgtaagcgc atgcttgctt ccgccggtga gctccagaag ggtaacgagc ttgccctccc    18480

ctccaagtac gtcaacttcc tctacctggc ctcccactac gagaagctca agggctctcc    18540

cgaggacaac gagcagaagc agctctttgt cgagcagcac aagcactacc tggatgagat    18600

catcgagcag atctccgagt tcagcaagcg tgtcatcctg gccgatgcca accttgacaa    18660

ggtcctctct gcctacaaca agcaccgtga caagcccatc cgcgagcagg cggagaacat    18720

catccacctg ttcaccctca ccaacctggg tgctcctgct gctttcaagt actttgacac    18780

caccatcgac cgcaagcgtt acacctccac caaggaagtg cttgatgcga ctctgatcca    18840

ccagtcgatt accggtctgt acgagactcg tatcgacctg tctcagctcg gtggtgactc    18900

tcgtgccgat cccaagaaga agcgcaaggt ttaaacaagc gcttagctcg aacaaaagaa    18960

aaagtaaaaa cggttaatag cattggattc cgaactacaa agtataaact agtttcactc    19020

cttgtagaag ccagatacgg gccggggtag ataccgcgca ctccctcagc agcctcgcag    19080

tcatggtcag catcgaagaa ctcccacaca aaatgcgctg ggagaagagt tcgaaatgca    19140

gctgggctca attagccgtc gtacacggag atagatactg aatacatacc cgtttgagcc    19200

tgaaaatttt tgacattcgt gcccatacca tgaacctcgt ac                       19242


<210>  83
<211>  19569
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BG-AMA17 AMA hygB/Cas9 st

<400>  83
ggtaccgagg ttcatggtat gggcacgaat gtcaaaaatt ttcaggctca aacgggtatg       60

tattcagtat ctatctccgt gtacgacggc taattgagcc cagctgcatt tcgaactctt      120

ctcccagcgc attttgtgtg ggagttcttc gatgctgacc atgactgcga ggctgctgag      180

ggagtgcgcg gtatctaccc cggcccgtat ctggcttcta caaggagtga aactagttta      240

tactttgtag ttcggaatcc aatgctatta accgttttta ctttttcttt tgttcgagct      300

aagcgcttgt ttaaaccttg cgcttcttct tgggatcggc acgagagtca ccaccgagct      360

gagacaggtc gatacgagtc tcgtacagac cggtaatcga ctggtggatc agagtcgcat      420

caagcacttc cttggtggag gtgtaacgct tgcggtcgat ggtggtgtca aagtacttga      480

aagcagcagg agcacccagg ttggtgaggg tgaacaggtg gatgatgttc tccgcctgct      540

cgcggatggg cttgtcacgg tgcttgttgt aggcagagag gaccttgtca aggttggcat      600

cggccaggat gacacgcttg ctgaactcgg agatctgctc gatgatctca tccaggtagt      660

gcttgtgctg ctcgacaaag agctgcttct gctcgttgtc ctcgggagag cccttgagct      720

tctcgtagtg ggaggccagg tagaggaagt tgacgtactt ggaggggagg gcaagctcgt      780

tacccttctg gagctcaccg gcggaagcaa gcatgcgctt acggccgttc tccagctcga      840

acagagagta cttggggagc ttgatgatga ggtccttctt gacctctttg taacccttgg      900

cctccaggaa gtcgataggg ttcttctcga agctgctgcg ttccatgatg gtgataccga      960

ggagttcctt cacactcttc agcttcttgc tcttgccctt ctcgaccttg gcgacaacca     1020

agacggagta ggcaacggta ggcgaatcga atccaccgta cttcttgggg tcccaatcct     1080

tcttgcgagc gatcagcttg tcggagttgc gcttgggaag aatcgactcc ttggagaaac     1140

caccagtctg cacctcggtc ttcttgacaa tgttgacctg gggcatagaa aggaccttgc     1200

ggacggtagc gaagtcacgg cccttgtccc agacgatctc accggtttcg ccgttagtct     1260

caatcaaggg acgcttgcga atctcgccgt tggccaatgt tatttcggtc ttgaagaagt     1320

tcatgatgtt gctgtagaag aagtacttgg cagtggcctt tccgatctcc tgctcggact     1380

tagcgatcat cttgcgcacg tcgtacacct tgtagtcacc gtagacgaac tcggactcga     1440

gcttgggata cttcttaatc aaagcagtac cgacgacggc gttgaggtag gcatcgtgag     1500

cgtggtggta gttgttgatc tcgcggacct tgtagaactg gaagtctttc ctaaagtcac     1560

tgaccagctt gctctttaag gtgatgacct tgacctcacg gatgagcttg tcgttctcgt     1620

cgtacttggt gttcatgcga gagtccagga tctgggcaac gtgcttagtg atctgacggg     1680

tttcaaccaa ttgacgcttg atgaaaccgg ccttgtcgag ctcggagagt ccgccacgct     1740

cggccttggt aaggttgtcg aacttgcgct gagtgatgag cttagcgttg agcaattgtc     1800

tccagtagtt cttcatcttc ttaacaacct cttccgaggg gacgttatcg ctcttgccac     1860

gattcttgtc ggaacgggtg agcaccttgt tgtcaatgct gtcatccttg aggaaggact     1920

gggggacgat gtggtcaaca tcgtagtcgg agaggcggtt gatatccagc tcctggtcga     1980

cgtacatgtc acgtccgttc tgcaagtagt agaggtagag cttctcattc tgcagctggg     2040

tgttctcgac ggggtgttcc ttgagaatct gggaaccgag ctccttgatg ccttcctcga     2100

tacgcttcat gcgctcacgg ctgttcttct gacccttctg agtggtctgg ttctcacgag     2160

ccatttcgat gacgatgttc tcgggcttgt gacgacccat gaccttcaca agctcgtcaa     2220

caaccttcac agtctgcagg atacccttct tgatagcggg ggaaccagcc aggttggcaa     2280

tgtgttcgtg tagcgagtca ccctggccgg agacttgggc cttctggatg tcctctttga     2340

aggtcagaga gtcgtcgtgg atgagctgca tgaagttgcg gttggcgaag ccgtcggact     2400

tcaggaagtc aaggatggtc ttgccagact gcttatcacg gataccgtta atcaacttgc     2460

gagagaggcg accccagcca gtgtaacggc ggcgcttcaa ctgcttcatg accttgtcgt     2520

cgaagaggtg agcgtaggtc ttcagacgtt cttcgatcat ctcacggtcc tcgaagaggg     2580

tgagggtaag aacaatgtcc tcaagaatgt cttcgttctc ctcgttgtca aggaagtcct     2640

tgtccttgat gatcttgagc agatcgtggt aggtgccgag agaagcgttg aagcggtcct     2700

caacaccgga gatctcaacg gagtcaaagc actcgatctt cttgaagtag tcttccttga     2760

gctgcttgac ggtcaccttg cggttagtct tgaacagcag gtcgacaatg gccttcttct     2820

gttcgccgga gaggaaagca ggcttgcgca tgccctcggt cacgtacttg accttggtca     2880

gctcgttgta gacagtgaag tactcgtaca agagggagtg cttgggcagg actttctcgt     2940

tgggcaggtt cttgtcgaag ttggtcatac gctcaatgaa gctctgagcg gaggcaccct     3000

tgtcgacgac ttcctcgaag ttccaggggg tgatggtttc ctcggacttg cgggtcatcc     3060

aggcgaagcg ggagttaccg cgagccagag gaccgacgta gtaggggata cggaaggtca     3120

ggatcttctc aatcttctca cggttgtcct tcaagaaagg gtagaagtct tcctggcgac     3180

gaaggatggc gtgcaattca cccaggtgga tctggtgggg gatgctgccg ttgtcgaaag     3240

tacgctgctt gcgcagaagg tcctcacggt tcagcttaac aagaagctcc tcagtgccat     3300

ccatcttctc gaggataggc ttaatgaact tgtagaattc ttcctgggaa gcaccaccgt     3360

cgatgtaacc ggcgtagccg ttcttggact ggtcgaagaa gatctccttg tacttttcgg     3420

ggagctgctg gcggaccaga gccttgagta gggtgaggtc ctggtggtgc tcatcgtatc     3480

tcttgatcat agaggcggag agaggggcct tggtgatctc agtgttgaca cggaggatat     3540

ctgacaggag aatggcatcg gagaggttct tggcagctag gaagaggtcg gcgtactgat     3600

ctcctatctg ggcgagaagg ttatcaaggt cgtcgtcgta ggtatccttg gaaagttgta     3660

acttagcatc ctcagcaaga tcgaagttgc tcttgaagtt gggagtcagt ccgagggaca     3720

gggcaataag gttgccgaaa agaccgttct tcttctcacc agggagctgg gcaatcaagt     3780

tctcaagacg gcgggacttg ctcaggcgag cggagaggat ggccttggca tccacgccag     3840

acgcgttgat ggggttttcc tcgaaaagct ggttgtaggt ctgaacgagc tggatgaaga     3900

gtttatcaac atcggagttg tcggggttga ggtcaccctc gatcaggaag tgaccacgga     3960

acttgatcat gtgcgccaga gccaggtaaa tgaggcggag gtcagccttg tcggtgctgt     4020

cgacgagctt tttgcgtagg tggtagatgg tggggtactt ctcgtggtaa gcgacctcat     4080

cgacaatgtt accgaagata gggtgacgct cgtgcttctt gtcttcttca acaaggaacg     4140

actcctccag acggtggaag aaagagtcat caaccttggc catctcgttg gagaaaatct     4200

cctggaggta gcagatacgg ttcttgcggc gagtgtaacg acggcgagcg gtacgcttca     4260

gacgggtggc ctcagcagtc tcaccggagt cgaagagaag cgcaccaatg aggttcttct     4320

tgatggagtg acgatcggtg tttcccagga ccttgaattt cttgctagga accttgtact     4380

cgtcggtgat gacagcccag ccaacggagt tggtgccaat gtccagaccg atgctatact     4440

tcttgtccat tttgacggtg gaaggtgagt tggggttggt gtcatcgtgg gggaagaact     4500

tggcttttat atgggtgcag gtgaggggac ttaagccacg tgaaagttca ttcgagagag     4560

ctaaggcata ttaatgcaca tgtgtgggag ttgcatggaa cttgcatgaa aggtgcatga     4620

aaggtgcatg gtattgcaga atgcgctcgg gggtctgcgg agaaatccgt taggaaaaga     4680

tcgtcatcct tctgctgcat caccgttagc ttgaaattta gttccagcgc tagtcaaggg     4740

cttcagttca gattctgcaa gtatcaggtc catcattact ctcttcagca ggcggatcga     4800

atatcccccg aggcacatgg gaggtcttat tatccgatcg ttgatcacca tgccaatcgc     4860

ttcgaccgac cacaagttgc atcaagcact aactgcctca agcagatgcc gagtcttcat     4920

ctccgatatt taatcccgtt gaatctccgc cccctgtcat ctccaccgtt taatctgggg     4980

tggtggcgga tgtccaccaa ttagccggct aaattatccc catcgtcagc acgctagacc     5040

tgccttggaa ctagcgcttt ggtgagaaat ctcttggttg tgagtctgat accacattcc     5100

ttgacttcca tgttgttctg gaggtgtgaa agtataaaca atgccacaga tggactaatc     5160

tccggagaga tgaccctctt caagactggt gcagtgccta ggatcgctag tatcccaaaa     5220

cttcggggct gccttcattt ccagagagtt gcggtacctt gcccatcgaa cgtacaagta     5280

ctcctctgtt ctctccttcc tttgctttgt gcggagaccg gcttactaaa agccagataa     5340

cagtatgcat atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc     5400

cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg     5460

acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac     5520

catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg     5580

gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg     5640

ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg     5700

tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca     5760

gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg     5820

atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtttcc gttatcgggg     5880

aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt     5940

tctggggaat ataaggtctc gcctccggat cgatgtacac aaccgactgc acccaaacga     6000

acacaaatct tagcagtgcc ctcgccggat agcttggact gtcctttacc gtcgccagca     6060

caagaagggt atctctgagg tccgtaccgc cttttcttta ccactggatt cgattttcgc     6120

agttggaatg atacatctgg ggactgcgaa tggtttaccc ctcggccgat actatgggtc     6180

gtgaagagat ggaacattcc gaaagtgttt tgcggataac attggtggca tcgaaaacag     6240

aatgctgacc attgatttca acacgaacag gaggttgcca agaagcgtac ccgccgtgtc     6300

gtcaagtccc agcgtgccat cgtcggtgct tccctcgacg tgatcaagga gcgccgctcc     6360

cagcgccccg aggcccgtgc cgccgcccgc cagcaggcca tcaaggacgc caaggagaag     6420

aaggctgccg ctgagtccaa gaagaaggct gagaaggcta agaacgccgc tgctggtgcc     6480

aagggtgctg ctcagcgcat ccagagcaag cagggtgcta agggttctgc tcccaaggtc     6540

gctgccaagt ctcgttaagg aatgaataac ggttcggctt gggattgggt gcggaaggca     6600

agagtttcat ggacgaattt tgggaggtta ctggagctgg aatatgtgtt ttccctacca     6660

ccaaaaatga aatgttccaa aactatcggc gtgcaagacg gcctcttacg ggtttaacgg     6720

ctctcagata agctctatca atcgcgccac ggatgcatga atgaagatcc agatggccgc     6780

gggatatatc gtgctagtgt aattcctaca tgatcttgct gttcactcca tgcgcatcca     6840

gatattccag gggtcgactg ttaattgata tgcctgggct tgagactccg tagacgccca     6900

gtcaatgtgc aattaatacg agggtgctgt tatcggcagc aaccttgtac ttctccataa     6960

gatgggggaa tgccatggac ctgagtgatc aattgacgca agtctcccat aacgcggcgg     7020

cttgacctaa aatccatata ccgccccgtt gagcctccgc gctccagagt cctgtcccgg     7080

aatagggcac aaacctaggc taacctaatt cgtcgtccgc gtctgagttc agacaaaaga     7140

acttccaagt atcagcagag tacgctgata ttgataagta ggcaaacata agaccaataa     7200

gcaagtagaa taaaaaatta taaggacact gcctccataa agcgccctcc caagacctca     7260

gggacaaaac ttctcaagtg gcaattcact gcctcaggcc gtgtccagtg aagtgacgaa     7320

gcgacactgt tgcctgctga ctcagccgct ttccgccctg ccgaatttgc catctcgctt     7380

acaggtcagc actagcgcga ttcgcccaca gatgctcagc gcaaagtggt gactcagtca     7440

aaccccccct acaagattcc acctcgattt ttcaacttcc catctcgatc cgacaagttc     7500

tacatccacc gtcaaaatgg cctccagcga agatgtcatc aaggagttca tgcgcttcaa     7560

ggtccgcatg gaaggatccg tcaacggcca cgagttcgag attgagggtg agggtgaggg     7620

ccgcccctac gaaggcaccc agactgccaa gctcaaggtc accaagggtg gtcctctccc     7680

cttcgcttgg gatatcctgt ctcctcagtt ccagtacggc tccaaggtct acgtcaagca     7740

ccccgccgac atccccgact acaagaagct ttctttcccc gagggtttca agtgggagcg     7800

tgtcatgaac ttcgaggatg gtggtgttgt gaccgttact caggacagca gcttgcagga     7860

tggctctttc atctacaagg tcaagttcat tggtgtcaac ttcccctccg acggccctgt     7920

catgcagaag aagaccatgg gctgggaagc gtcgactgag cgtctgtacc cccgtgacgg     7980

tgttctcaag ggtgagatcc acaaggctct caagctcaag gacggtggtc actaccttgt     8040

tgagttcaag tccatctaca tggccaagaa gcctgtgcag ctgcccggat actactacgt     8100

ggactccaag cttgacatca cctcccacaa cgaagactac accattgttg agcagtacga     8160

gcgtgctgag ggccgccacc acctcttcct gacccacgga atggatgagc tgtacaagtc     8220

gaaactataa ataaatggtt tgcgttgcga ttgactgaaa cgaaaaaaag cgaaaatgat     8280

tctgggaatg aattgataaa gcgcgggctc tgcggtacgg ttacggttgc ggtcgcggac     8340

gaatggactg ggctgagctg ggctggagga agtccatcga acaaggacaa ggggtggaat     8400

atggcacggg tcgattttgt tatacatacc ctaccatcca tctatccatt taaataccaa     8460

atgagttgtt gaatggattc gcggtcttct cggtttattt ttgcttgctt gcgtgcttaa     8520

gggatagtgt gcctcacgct ttccggcatc ttccagacca cagtatatcc atccgcctcc     8580

tgttgaagct tattttttgt atactgtttt gtgatagcac gaagtttttc cacggtatct     8640

tgttaaaaat atatatttgt ggcgggctta cctacatcaa attaataaga gactaattat     8700

aaactaaaca cacaagcaag ctactttagg gtaaaagttt ataaatgctt ttgacgtata     8760

aacgttgctt gtatttatta ttacaattaa aggtggatag aaaacctaga gactagttag     8820

aaactaatct caggtttgcg ttaaactaaa tcagagcccg agaggttaac agaacctaga     8880

aggggactag atatccgggt agggaaacaa aaaaaaaaaa caagacagcc acatattagg     8940

gagactagtt agaagctagt tccaggacta ggaaaataaa agacaatgat accacagtct     9000

agttgacaac tagatagatt ctagattgag gccaaagtct ctgagatcca ggttagttgc     9060

aactaatact agttagtatc tagtctccta taactctgaa gctagaataa cttactacta     9120

ttatcctcac cactgttcag ctgcgcaaac ggagtgattg caaggtgttc agagactagt     9180

tattgactag tcagtgacta gcaataacta acaaggtatt aacctaccat gtctgccatc     9240

accctgcact tcctcgggct cagcagcctt ttcctcctca ttttcatgct cattttcctt     9300

gtttaagact gtgactagtc aaagactagt ccagaaccac aaaggagaaa tgtcttacca     9360

ctttcttcat tgcttgtctc ttttgcatta tccatgtctg caactagtta gagtctagtt     9420

agtgactagt ccgacgagga cttgcttgtc tccggattgt tggaggaact ctccagggcc     9480

tcaagatcca caacagagcc ttctagaaga ctggtcaata actagttggt ctttgtctga     9540

gtctgactta cgaggttgca tactcgctcc ctttgcctcg tcaatcgatg agaaaaagcg     9600

ccaaaactcg caatatggct ttgaaccaca cggtgctgag actagttaga atctagtccc     9660

aaactagctt ggatagctta cctttgccct ttgcgttgcg acaggtcttg cagggtatgg     9720

ttcctttctc accagctgat ttagctgcct tgctaccctc acggcggatc tgcataaaga     9780

gtggctagag gttataaatt agcactgatc ctaggtacgg ggctgaatgt aacttgcctt     9840

tcctttctca tcgcgcggca agacaggctt gctcaaattc ctaccagtca caggggtatg     9900

cacggcgtac ggaccacttg aactagtcac agattagtta gcaactagtc tgcattgaat     9960

ggctgtactt acgggccctc gccattgtcc tgatcatttc cagcttcacc ctcgttgctg    10020

caaagtagtt agtgactagt caaggactag ttgaaatggg agaagaaact cacgaattct    10080

cgacaccctt agtattgtgg tccttggact tggtgctgct atatattagc taatacacta    10140

gttagactca cagaaactta cgcagctcgc ttgcgcttct tggtaggagt cggggttggg    10200

agaacagtgc cttcaaacaa gccttcatac catgctactt gactagtcag ggactagtca    10260

ccaagtaatc tagataggac ttgcctttgg cctccatcag ttccttcata gtgggaggtc    10320

cattgtgcaa tgtaaactcc atgccgtggg agttcttgtc cttcaagtgc ttgaccaata    10380

tgtttctgtt ggcagaggga acctgtcaac tagttaataa ctagtcagaa actagtatag    10440

cagtagactc actgtacgct tgaggcatcc cttcactcgg cagtagactt catatggatg    10500

gatatcaggc acgccattgt cgtcctgtgg actagtcagt aactaggctt aaagctagtc    10560

gggtcggctt actatcttga aatccggcag cgtaagctcc ccgtccttaa ctgcctcgag    10620

atagtgacag tactctgggg actttcggag atcgttatcg cgaatgctcg gcatactaat    10680

cgttgactag tcttggacta gtcccgagca aaaaggattg gaggaggagg aggaaggtga    10740

gagtgagaca aagagcgaaa taagagcttc aaaggctatc tctaagcagt atgaaggtta    10800

agtatctagt tcttgactag atttaaaaga gatttcgact agttatgtac ctggagtttg    10860

gatataggaa tgtgttgtgg taacgaaatg taagggggag gaaagaaaaa gtcggtcaag    10920

aggtaactct aagtcggcca ttcctttttg ggaggcgcta accataaacg gcatggtcga    10980

cttagagtta gctcagggaa tttagggagt tatctgcgac caccgaggaa cggcggaatg    11040

ccaaagaatc ccgatggagc tctagctggc ggttgacaac cccacctttt ggcgtttctg    11100

cggcgttgca ggcgggactg gatacttcgt agaaccagaa aggcaaggca gaacgcgctc    11160

agcaagagtg ttggaagtga tagcatgatg tgccttgtta actaggtcaa aatctgcagt    11220

atgcttgatg ttatccaaag tgtgagagag gaaggtccaa acatacacga ttgggagagg    11280

gcctaggtat aagagttttt gagtagaacg catgtgagcc cagccatctc gaggagatta    11340

aacacgggcc ggcatttgat ggctatgtta gtaccccaat ggaaagcctg agagtccagt    11400

ggtcgcagat aactccctaa attccctgag ctaactctaa gtcgaccatg ccgtttatgg    11460

ttagcgcctc ccaaaaagga atggccgact tagagttacc tcttgaccga ctttttcttt    11520

cctccccctt acatttcgtt accacaacac attcctatat ccaaactcca ggtacataac    11580

tagtcgaaat ctcttttaaa tctagtcaag aactagatac ttaaccttca tactgcttag    11640

agatagcctt tgaagctctt atttcgctct ttgtctcact ctcaccttcc tcctcctcct    11700

ccaatccttt ttgctcggga ctagtccaag actagtcaac gattagtatg ccgagcattc    11760

gcgataacga tctccgaaag tccccagagt actgtcacta tctcgaggca gttaaggacg    11820

gggagcttac gctgccggat ttcaagatag taagccgacc cgactagctt taagcctagt    11880

tactgactag tccacaggac gacaatggcg tgcctgatat ccatccatat gaagtctact    11940

gccgagtgaa gggatgcctc aagcgtacag tgagtctact gctatactag tttctgacta    12000

gttattaact agttgacagg ttccctctgc caacagaaac atattggtca agcacttgaa    12060

ggacaagaac tcccacggca tggagtttac attgcacaat ggacctccca ctatgaagga    12120

actgatggag gccaaaggca agtcctatct agattacttg gtgactagtc cctgactagt    12180

caagtagcat ggtatgaagg cttgtttgaa ggcactgttc tcccaacccc gactcctacc    12240

aagaagcgca agcgagctgc gtaagtttct gtgagtctaa ctagtgtatt agctaatata    12300

tagcagcacc aagtccaagg accacaatac taagggtgtc gagaattcgt gagtttcttc    12360

tcccatttca actagtcctt gactagtcac taactacttt gcagcaacga gggtgaagct    12420

ggaaatgatc aggacaatgg cgagggcccg taagtacagc cattcaatgc agactagttg    12480

ctaactaatc tgtgactagt tcaagtggtc cgtacgccgt gcatacccct gtgactggta    12540

ggaatttgag caagcctgtc ttgccgcgcg atgagaaagg aaaggcaagt tacattcagc    12600

cccgtaccta ggatcagtgc taatttataa cctctagcca ctctttatgc agatccgccg    12660

tgagggtagc aaggcagcta aatcagctgg tgagaaagga accataccct gcaagacctg    12720

tcgcaacgca aagggcaaag gtaagctatc caagctagtt tgggactaga ttctaactag    12780

tctcagcacc gtgtggttca aagccatatt gcgagttttg gcgctttttc tcatcgattg    12840

acgaggcaaa gggagcgagt atgcaacctc gtaagtcaga ctcagacaaa gaccaactag    12900

ttattgacca gtcttctaga aggctctgtt gtggatcttg aggccctgga gagttcctcc    12960

aacaatccgg agacaagcaa gtcctcgtcg gactagtcac taactagact ctaactagtt    13020

gcagacatgg ataatgcaaa agagacaagc aatgaagaaa gtggtaagac atttctcctt    13080

tgtggttctg gactagtctt tgactagtca cagtcttaaa caaggaaaat gagcatgaaa    13140

atgaggagga aaaggctgct gagcccgagg aagtgcaggg tgatggcaga catggtaggt    13200

taataccttg ttagttattg ctagtcactg actagtcaat aactagtctc tgaacacctt    13260

gcaatcactc cgtttgcgca gctgaacagt ggtgaggata atagtagtaa gttattctag    13320

cttcagagtt ataggagact agatactaac tagtattagt tgcaactaac ctggatctca    13380

gagactttgg cctcaatcta gaatctatct agttgtcaac tagactgtgg tatcattgtc    13440

ttttattttc ctagtcctgg aactagcttc taactagtct ccctaatatg tggctgtctt    13500

gttttttttt tttgtttccc tacccggata tctagtcccc ttctaggttc tgttaacctc    13560

tcgggctctg atttagttta acgcaaacct gagattagtt tctaactagt ctctaggttt    13620

tctatccacc tttaattgta ataataaata caagcaacgt ttatacgtca aaagcattta    13680

taaactttta ccctaaagta gcttgcttgt gtgtttagtt tataattagt ctcttattaa    13740

tttgatgtag gtaagcccgc cacaaatata tatttttaac aagataccgt ggaaaaactt    13800

cgtgctatca caaaacagta tacaaaaaat aagctatcga attcctgcag agatcatcct    13860

gtcttcagtc ttaagacttc tctcctatat cacccgcact taccctagag tgccgcttag    13920

gtgctaaggg cacattgagt attggccgtg tagaatatat agcttaagta cggccaagca    13980

gacgggaagc cctgttctcc acaccctatg gtcgtatata tcaggcttct accgggaaac    14040

gattaagagt gtataatgga ctgaaaatca atatgaacgg gacaatgctc aagttaaatt    14100

agttaggcat cctaatctct actaaatgtt ctatctagag atcggggtac tataggcccg    14160

tacgttaatc actctacgct tctctccctt aggtatagtg taggtagggg ctagacattt    14220

atatgagtca gatggtacaa acggtaggca gtgcgggcga agaagtgaag acggagtcgg    14280

ttgaagctac atacaaaaga tgcattggct cgtcatgaag agcctcccgg gtttattcct    14340

ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc    14400

catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc    14460

cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt    14520

caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc cggagccgcg    14580

gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag    14640

ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg    14700

cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc    14760

cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct    14820

catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat    14880

acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc    14940

cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat    15000

ccatggcctc cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc aggtcttgca    15060

acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgctg aattccccaa    15120

tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat    15180

ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat    15240

cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt    15300

cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc attttgacgg    15360

tgggatcctg tgatgtctgc tcaagcgggg tagctgttag tcaagctgcg atgaagtggg    15420

aaagctcgaa ctgaaaggtt caaaggaata agggatggga aggatggagt atggatgtag    15480

caaagtactt acttagggga aataaaggtt cttggatggg aagatgaata tactgaagat    15540

gggaaaagaa agagaaaaga aaagagcagc tggtggggag agcaggaaaa tatggcaaca    15600

aatgttggac tgacgcaacg accttgtcaa ccccgccgac acaccgggcg gacagacggg    15660

gcaaagctgc ctaccaggga ctgagggacc tcagcaggtc gagtgcagag caccggatgg    15720

gtcgactgcc agcttgtgtt cccggtctgc gccgctggcc agctcctgag cggcctttcc    15780

ggtttcatac accgggcaaa gcaggagagg cacgatattt ggacgcccta cagatgccgg    15840

atgggccaat tagggagctt acgcgccggg tactcgctct acctacttcg gagaaggtac    15900

tatctcgtga atcttttacc agatcggaag caattggact tctgtaccta ggttaatggc    15960

atgctatttc gccgacggct atacacccct ggcttcacat tctccttcgc ttactgccgg    16020

tgattcgatg aagctccata ttctccgatg atgcaataga ttcttggtca acgaggggca    16080

caccagcctt tccacttcgg ggcggagggg cggccggtcc cggattaata atcatccact    16140

gcacctcaga gccgccagag ctgtctggcg cagtggcgct tattactcag cccttctctc    16200

tgcgtccgtc cgtctctccg catgccagaa agagtcaccg gtcactgtac agagcggccg    16260

ccaccgcggt ggagctccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc    16320

gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca    16380

gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc    16440

caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg    16500

gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct    16560

cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta    16620

aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa    16680

cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct    16740

ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc    16800

aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg    16860

ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt    16920

acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct    16980

aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat    17040

attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg    17100

cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg    17160

aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc    17220

ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttcga ccgaataaat    17280

acctgtgacg gaagatcact tcgcagaata aataaatcct ggtgtccctg ttgataccgg    17340

gaagccctgg gccaactttt ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa    17400

ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttgt cgagattttc    17460

aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc    17520

ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa    17580

ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa    17640

gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattacg    17700

tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt    17760

tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg    17820

gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt    17880

ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac    17940

cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg    18000

caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc    18060

cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga    18120

gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggttg    18180

ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag caaattcgac    18240

ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg ctggtttacc    18300

ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg aggccagttt    18360

gctcaggctc tccccgtgga ggtaataatt gacgatatga tccttttttt ctgatcaaaa    18420

aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt    18480

tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt    18540

tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt    18600

ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag    18660

ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta    18720

gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat    18780

aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg    18840

ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg    18900

agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac    18960

aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga    19020

aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt    19080

ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta    19140

cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat    19200

tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg    19260

accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct    19320

ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa    19380

gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct    19440

ttacacttta tgctcccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac    19500

acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa    19560

caaaagctg                                                            19569


<210>  84
<211>  13672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BG-AMA1 AMA phleo / no Cas9 expression cassette

<400>  84
ggtaccttgc ccatcgaacg tacaagtact cctctgttct ctccttcctt tgctttgtgc       60

ggagaccggc ttactaaaag ccagataaca gtatgcatat ttgcgcgctg atttttgcgg      120

tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag      180

cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg      240

tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg      300

aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga      360

acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat      420

aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc      480

gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt      540

gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg      600

gccagtgtgc cggtttccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat      660

gacatcaaaa acgccattaa cctgatgttc tggggaatat aaggtctcgc ctccggatcg      720

atgtacacaa ccgactgcac ccaaacgaac acaaatctta gcagtgccct cgccggatag      780

cttggactgt cctttaccgt cgccagcaca agaagggtat ctctgaggtc cgtaccgcct      840

tttctttacc actggattcg attttcgcag ttggaatgat acatctgggg actgcgaatg      900

gtttacccct cggccgatac tatgggtcgt gaagagatgg aacattccga aagtgttttg      960

cggataacat tggtggcatc gaaaacagaa tgctgaccat tgatttcaac acgaacagga     1020

ggttgccaag aagcgtaccc gccgtgtcgt caagtcccag cgtgccatcg tcggtgcttc     1080

cctcgacgtg atcaaggagc gccgctccca gcgccccgag gcccgtgccg ccgcccgcca     1140

gcaggccatc aaggacgcca aggagaagaa ggctgccgct gagtccaaga agaaggctga     1200

gaaggctaag aacgccgctg ctggtgccaa gggtgctgct cagcgcatcc agagcaagca     1260

gggtgctaag ggttctgctc ccaaggtcgc tgccaagtct cgttaaggaa tgaataacgg     1320

ttcggcttgg gattgggtgc ggaaggcaag agtttcatgg acgaattttg ggaggttact     1380

ggagctggaa tatgtgtttt ccctaccacc aaaaatgaaa tgttccaaaa ctatcggcgt     1440

gcaagacggc ctcttacggg tttaacggct ctcagataag ctctatcaat cgcgccacgg     1500

atgcatgaat gaagatccag atggccgcgg gatatatcgt gctagtgtaa ttcctacatg     1560

atcttgctgt tcactccatg cgcatccaga tattccaggg gtcgactgtt aattgatatg     1620

cctgggcttg agactccgta gacgcccagt caatgtgcaa ttaatacgag ggtgctgtta     1680

tcggcagcaa ccttgtactt ctccataaga tgggggaatg ccatggacct gagtgatcaa     1740

ttgacgcaag tctcccataa cgcggcggct tgacctaaaa tccatatacc gccccgttga     1800

gcctccgcgc tccagagtcc tgtcccggaa tagggcacaa acctaggcta acctaattcg     1860

tcgtccgcgt ctgagttcag acaaaagaac ttccaagtat cagcagagta cgctgatatt     1920

gataagtagg caaacataag accaataagc aagtagaata aaaaattata aggacactgc     1980

ctccataaag cgccctccca agacctcagg gacaaaactt ctcaagtggc aattcactgc     2040

ctcaggccgt gtccagtgaa gtgacgaagc gacactgttg cctgctgact cagccgcttt     2100

ccgccctgcc gaatttgcca tctcgcttac aggtcagcac tagcgcgatt cgcccacaga     2160

tgctcagcgc aaagtggtga ctcagtcaaa ccccccctac aagattccac ctcgattttt     2220

caacttccca tctcgatccg acaagttcta catccaccgt caaaatggcc tccagcgaag     2280

atgtcatcaa ggagttcatg cgcttcaagg tccgcatgga aggatccgtc aacggccacg     2340

agttcgagat tgagggtgag ggtgagggcc gcccctacga aggcacccag actgccaagc     2400

tcaaggtcac caagggtggt cctctcccct tcgcttggga tatcctgtct cctcagttcc     2460

agtacggctc caaggtctac gtcaagcacc ccgccgacat ccccgactac aagaagcttt     2520

ctttccccga gggtttcaag tgggagcgtg tcatgaactt cgaggatggt ggtgttgtga     2580

ccgttactca ggacagcagc ttgcaggatg gctctttcat ctacaaggtc aagttcattg     2640

gtgtcaactt cccctccgac ggccctgtca tgcagaagaa gaccatgggc tgggaagcgt     2700

cgactgagcg tctgtacccc cgtgacggtg ttctcaaggg tgagatccac aaggctctca     2760

agctcaagga cggtggtcac taccttgttg agttcaagtc catctacatg gccaagaagc     2820

ctgtgcagct gcccggatac tactacgtgg actccaagct tgacatcacc tcccacaacg     2880

aagactacac cattgttgag cagtacgagc gtgctgaggg ccgccaccac ctcttcctga     2940

cccacggaat ggatgagctg tacaagtcga aactataaat aaatggtttg cgttgcgatt     3000

gactgaaacg aaaaaaagcg aaaatgattc tgggaatgaa ttgataaagc gcgggctctg     3060

cggtacggtt acggttgcgg tcgcggacga atggactggg ctgagctggg ctggaggaag     3120

tccatcgaac aaggacaagg ggtggaatat ggcacgggtc gattttgtta tacataccct     3180

accatccatc tatccattta aataccaaat gagttgttga atggattcgc ggtcttctcg     3240

gtttattttt gcttgcttgc gtgcttaagg gatagtgtgc ctcacgcttt ccggcatctt     3300

ccagaccaca gtatatccat ccgcctcctg ttgaagctta ttttttgtat actgttttgt     3360

gatagcacga agtttttcca cggtatcttg ttaaaaatat atatttgtgg cgggcttacc     3420

tacatcaaat taataagaga ctaattataa actaaacaca caagcaagct actttagggt     3480

aaaagtttat aaatgctttt gacgtataaa cgttgcttgt atttattatt acaattaaag     3540

gtggatagaa aacctagaga ctagttagaa actaatctca ggtttgcgtt aaactaaatc     3600

agagcccgag aggttaacag aacctagaag gggactagat atccgggtag ggaaacaaaa     3660

aaaaaaaaca agacagccac atattaggga gactagttag aagctagttc caggactagg     3720

aaaataaaag acaatgatac cacagtctag ttgacaacta gatagattct agattgaggc     3780

caaagtctct gagatccagg ttagttgcaa ctaatactag ttagtatcta gtctcctata     3840

actctgaagc tagaataact tactactatt atcctcacca ctgttcagct gcgcaaacgg     3900

agtgattgca aggtgttcag agactagtta ttgactagtc agtgactagc aataactaac     3960

aaggtattaa cctaccatgt ctgccatcac cctgcacttc ctcgggctca gcagcctttt     4020

cctcctcatt ttcatgctca ttttccttgt ttaagactgt gactagtcaa agactagtcc     4080

agaaccacaa aggagaaatg tcttaccact ttcttcattg cttgtctctt ttgcattatc     4140

catgtctgca actagttaga gtctagttag tgactagtcc gacgaggact tgcttgtctc     4200

cggattgttg gaggaactct ccagggcctc aagatccaca acagagcctt ctagaagact     4260

ggtcaataac tagttggtct ttgtctgagt ctgacttacg aggttgcata ctcgctccct     4320

ttgcctcgtc aatcgatgag aaaaagcgcc aaaactcgca atatggcttt gaaccacacg     4380

gtgctgagac tagttagaat ctagtcccaa actagcttgg atagcttacc tttgcccttt     4440

gcgttgcgac aggtcttgca gggtatggtt cctttctcac cagctgattt agctgccttg     4500

ctaccctcac ggcggatctg cataaagagt ggctagaggt tataaattag cactgatcct     4560

aggtacgggg ctgaatgtaa cttgcctttc ctttctcatc gcgcggcaag acaggcttgc     4620

tcaaattcct accagtcaca ggggtatgca cggcgtacgg accacttgaa ctagtcacag     4680

attagttagc aactagtctg cattgaatgg ctgtacttac gggccctcgc cattgtcctg     4740

atcatttcca gcttcaccct cgttgctgca aagtagttag tgactagtca aggactagtt     4800

gaaatgggag aagaaactca cgaattctcg acacccttag tattgtggtc cttggacttg     4860

gtgctgctat atattagcta atacactagt tagactcaca gaaacttacg cagctcgctt     4920

gcgcttcttg gtaggagtcg gggttgggag aacagtgcct tcaaacaagc cttcatacca     4980

tgctacttga ctagtcaggg actagtcacc aagtaatcta gataggactt gcctttggcc     5040

tccatcagtt ccttcatagt gggaggtcca ttgtgcaatg taaactccat gccgtgggag     5100

ttcttgtcct tcaagtgctt gaccaatatg tttctgttgg cagagggaac ctgtcaacta     5160

gttaataact agtcagaaac tagtatagca gtagactcac tgtacgcttg aggcatccct     5220

tcactcggca gtagacttca tatggatgga tatcaggcac gccattgtcg tcctgtggac     5280

tagtcagtaa ctaggcttaa agctagtcgg gtcggcttac tatcttgaaa tccggcagcg     5340

taagctcccc gtccttaact gcctcgagat agtgacagta ctctggggac tttcggagat     5400

cgttatcgcg aatgctcggc atactaatcg ttgactagtc ttggactagt cccgagcaaa     5460

aaggattgga ggaggaggag gaaggtgaga gtgagacaaa gagcgaaata agagcttcaa     5520

aggctatctc taagcagtat gaaggttaag tatctagttc ttgactagat ttaaaagaga     5580

tttcgactag ttatgtacct ggagtttgga tataggaatg tgttgtggta acgaaatgta     5640

agggggagga aagaaaaagt cggtcaagag gtaactctaa gtcggccatt cctttttggg     5700

aggcgctaac cataaacggc atggtcgact tagagttagc tcagggaatt tagggagtta     5760

tctgcgacca ccgaggaacg gcggaatgcc aaagaatccc gatggagctc tagctggcgg     5820

ttgacaaccc caccttttgg cgtttctgcg gcgttgcagg cgggactgga tacttcgtag     5880

aaccagaaag gcaaggcaga acgcgctcag caagagtgtt ggaagtgata gcatgatgtg     5940

ccttgttaac taggtcaaaa tctgcagtat gcttgatgtt atccaaagtg tgagagagga     6000

aggtccaaac atacacgatt gggagagggc ctaggtataa gagtttttga gtagaacgca     6060

tgtgagccca gccatctcga ggagattaaa cacgggccgg catttgatgg ctatgttagt     6120

accccaatgg aaagcctgag agtccagtgg tcgcagataa ctccctaaat tccctgagct     6180

aactctaagt cgaccatgcc gtttatggtt agcgcctccc aaaaaggaat ggccgactta     6240

gagttacctc ttgaccgact ttttctttcc tcccccttac atttcgttac cacaacacat     6300

tcctatatcc aaactccagg tacataacta gtcgaaatct cttttaaatc tagtcaagaa     6360

ctagatactt aaccttcata ctgcttagag atagcctttg aagctcttat ttcgctcttt     6420

gtctcactct caccttcctc ctcctcctcc aatccttttt gctcgggact agtccaagac     6480

tagtcaacga ttagtatgcc gagcattcgc gataacgatc tccgaaagtc cccagagtac     6540

tgtcactatc tcgaggcagt taaggacggg gagcttacgc tgccggattt caagatagta     6600

agccgacccg actagcttta agcctagtta ctgactagtc cacaggacga caatggcgtg     6660

cctgatatcc atccatatga agtctactgc cgagtgaagg gatgcctcaa gcgtacagtg     6720

agtctactgc tatactagtt tctgactagt tattaactag ttgacaggtt ccctctgcca     6780

acagaaacat attggtcaag cacttgaagg acaagaactc ccacggcatg gagtttacat     6840

tgcacaatgg acctcccact atgaaggaac tgatggaggc caaaggcaag tcctatctag     6900

attacttggt gactagtccc tgactagtca agtagcatgg tatgaaggct tgtttgaagg     6960

cactgttctc ccaaccccga ctcctaccaa gaagcgcaag cgagctgcgt aagtttctgt     7020

gagtctaact agtgtattag ctaatatata gcagcaccaa gtccaaggac cacaatacta     7080

agggtgtcga gaattcgtga gtttcttctc ccatttcaac tagtccttga ctagtcacta     7140

actactttgc agcaacgagg gtgaagctgg aaatgatcag gacaatggcg agggcccgta     7200

agtacagcca ttcaatgcag actagttgct aactaatctg tgactagttc aagtggtccg     7260

tacgccgtgc atacccctgt gactggtagg aatttgagca agcctgtctt gccgcgcgat     7320

gagaaaggaa aggcaagtta cattcagccc cgtacctagg atcagtgcta atttataacc     7380

tctagccact ctttatgcag atccgccgtg agggtagcaa ggcagctaaa tcagctggtg     7440

agaaaggaac cataccctgc aagacctgtc gcaacgcaaa gggcaaaggt aagctatcca     7500

agctagtttg ggactagatt ctaactagtc tcagcaccgt gtggttcaaa gccatattgc     7560

gagttttggc gctttttctc atcgattgac gaggcaaagg gagcgagtat gcaacctcgt     7620

aagtcagact cagacaaaga ccaactagtt attgaccagt cttctagaag gctctgttgt     7680

ggatcttgag gccctggaga gttcctccaa caatccggag acaagcaagt cctcgtcgga     7740

ctagtcacta actagactct aactagttgc agacatggat aatgcaaaag agacaagcaa     7800

tgaagaaagt ggtaagacat ttctcctttg tggttctgga ctagtctttg actagtcaca     7860

gtcttaaaca aggaaaatga gcatgaaaat gaggaggaaa aggctgctga gcccgaggaa     7920

gtgcagggtg atggcagaca tggtaggtta ataccttgtt agttattgct agtcactgac     7980

tagtcaataa ctagtctctg aacaccttgc aatcactccg tttgcgcagc tgaacagtgg     8040

tgaggataat agtagtaagt tattctagct tcagagttat aggagactag atactaacta     8100

gtattagttg caactaacct ggatctcaga gactttggcc tcaatctaga atctatctag     8160

ttgtcaacta gactgtggta tcattgtctt ttattttcct agtcctggaa ctagcttcta     8220

actagtctcc ctaatatgtg gctgtcttgt tttttttttt tgtttcccta cccggatatc     8280

tagtcccctt ctaggttctg ttaacctctc gggctctgat ttagtttaac gcaaacctga     8340

gattagtttc taactagtct ctaggttttc tatccacctt taattgtaat aataaataca     8400

agcaacgttt atacgtcaaa agcatttata aacttttacc ctaaagtagc ttgcttgtgt     8460

gtttagttta taattagtct cttattaatt tgatgtaggt aagcccgcca caaatatata     8520

tttttaacaa gataccgtgg aaaaacttcg tgctatcaca aaacagtata caaaaaataa     8580

gctatcgaat tcctgcagag atcatcctgt cttcagtctt aagacttctc tcctatatca     8640

cccgcactta ccctagagtg ccgcttaggt gctaagggca cattgagtat tggccgtgta     8700

gaatatatag cttaagtacg gccaagcaga cgggaagccc tgttctccac accctatggt     8760

cgtatatatc aggcttctac cgggaaacga ttaagagtgt ataatggact gaaaatcaat     8820

atgaacggga caatgctcaa gttaaattag ttaggcatcc taatctctac taaatgttct     8880

atctagagat cggggtacta taggcccgta cgttaatcac tctacgcttc tctcccttag     8940

gtatagtgta ggtaggggct agacatttat atgagtcaga tggtacaaac ggtaggcagt     9000

gcgggcgaag aagtgaagac ggagtcggtt gaagctacat acaaaagatg cattggctcg     9060

tcatgaagag cctcccgggt ttagtcctgc tcctcggcca cgaagtgcac gcagttgccg     9120

gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc ggtcatggcc     9180

ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc gtacagctcg     9240

tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg gtcctggacc     9300

gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc ctccacgaag     9360

tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac gtcgcgcgcg     9420

gtgagcaccg gaacggcact ggtcaacttg gccattttga cggtgggatc ctgtgatgtc     9480

tgctcaagcg gggtagctgt tagtcaagct gcgatgaagt gggaaagctc gaactgaaag     9540

gttcaaagga ataagggatg ggaaggatgg agtatggatg tagcaaagta cttacttagg     9600

ggaaataaag gttcttggat gggaagatga atatactgaa gatgggaaaa gaaagagaaa     9660

agaaaagagc agctggtggg gagagcagga aaatatggca acaaatgttg gactgacgca     9720

acgaccttgt caaccccgcc gacacaccgg gcggacagac ggggcaaagc tgcctaccag     9780

ggactgaggg acctcagcag gtcgagtgca gagcaccgga tgggtcgact gccagcttgt     9840

gttcccggtc tgcgccgctg gccagctcct gagcggcctt tccggtttca tacaccgggc     9900

aaagcaggag aggcacgata tttggacgcc ctacagatgc cggatgggcc aattagggag     9960

cttacgcgcc gggtactcgc tctacctact tcggagaagg tactatctcg tgaatctttt    10020

accagatcgg aagcaattgg acttctgtac ctaggttaat ggcatgctat ttcgccgacg    10080

gctatacacc cctggcttca cattctcctt cgcttactgc cggtgattcg atgaagctcc    10140

atattctccg atgatgcaat agattcttgg tcaacgaggg gcacaccagc ctttccactt    10200

cggggcggag gggcggccgg tcccggatta ataatcatcc actgcacctc agagccgcca    10260

gagctgtctg gcgcagtggc gcttattact cagcccttct ctctgcgtcc gtccgtctct    10320

ccgcatgcca gaaagagtca ccggtcactg tacagagcgg ccgccaccgc ggtggagctc    10380

caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg    10440

tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc    10500

cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct    10560

gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac    10620

gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc    10680

ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt    10740

agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg    10800

ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac    10860

gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta    10920

ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat    10980

ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact    11040

tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg    11100

tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt    11160

atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct    11220

gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca    11280

cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc    11340

gaagaacgtt ttccaatgat gagcactttt cgaccgaata aatacctgtg acggaagatc    11400

acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc tgggccaact    11460

tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac cataatgaaa    11520

taagatcact accgggcgta ttttttgagt tgtcgagatt ttcaggagct aaggaagcta    11580

aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag    11640

aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg    11700

atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta    11760

ttcacattct tgcccgcctg atgaatgctc atccggaatt acgtatggca atgaaagacg    11820

gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg    11880

aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat    11940

attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg    12000

agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg    12060

tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag    12120

gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc    12180

atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt    12240

aattttttta aggcagttat tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg    12300

ataataagcg gatgaatggc agaaattcga aagcaaattc gacccggtcg tcggttcagg    12360

gcagggtcgt taaatagccg cttatgtcta ttgctggttt accggtttat tgactaccgg    12420

aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag tttgctcagg ctctccccgt    12480

ggaggtaata attgacgata tgatcctttt tttctgatca aaaaggatct aggtgaagat    12540

cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc    12600

agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg    12660

ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct    12720

accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct    12780

tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct    12840

cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg    12900

gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc    12960

gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga    13020

gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg    13080

cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta    13140

tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg    13200

ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg    13260

ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat    13320

taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc    13380

agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc    13440

gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa    13500

cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgctccc    13560

ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga    13620

ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tg            13672


<210>  85
<211>  1627
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SGIC DNA fragment I

<400>  85
ctgcgacagc ggattgggcg gagaagaaga caacccttca gatatattca ggtgcttttc       60

cctcacatgt tttgccgcac cagccatccc actatcaaaa agcgatgatg tttgagattg      120

tcgggtgtcc acatctttta gtgtgaatcg ctagtagaat ttgggatatt attgagcatc      180

atcccatgat agcgagtaca agccccgagt aaataccaac attgctatgc tgctgtgctg      240

ctatctagtt tgctacgttg gtcgttgacc tcacagggat ttccaccaaa aagtggaccg      300

ggcgggcgcc actcggccgt gccacagcag cctgagagcg gacaaataac aacagccgcc      360

tgccgcgggg ttcggttgca aacatgacca acaggccagg ccatcatcaa cccaccgctg      420

cgttgatgcc caggatttca gtccaataat ccacaattta ccaacggata gagctaggtg      480

aattagatag acaggagggc cagagggagg ggaccgagat gaaaaatttt cgatgaaaga      540

gtggtcaagg tggggtcgta gttcggcgct ccgagggcga ggaaccaagg aaaggcgagg      600

aaaggacagg ctgatcgcgc tgcgttgctg ggctgcaagc gtgtccagtt gagtctggaa      660

aaggctccgc cgtgaagatt ctgcgttggt cccgcacctg cgcggtgggg gcattacccc      720

tccatgtcca atgatttcaa gtcaaagcca agggttgaag cccgcccgct tagtcgcctt      780

ctcgcttgac ccctccatat aagtatttcc cctcctcccc ctcccacaaa tttttccttt      840

ccctttcctc cctcgtccgc ttcagtacgt atatcttccc cccctctctc ttccttctca      900

ctcttctctc cttctttctt gattcatcct ctctctaact gacttctttg ctcagcacct      960

ctacgcgttc tggccgtagt atctgagcaa tttttctaca gactttttct atctaattcc     1020

aaaaaagaac ttcgagttca ttcaccaccg tcaaaatgat ctgactgatg agtccgtgag     1080

gacgaaacga gtaagctcgt ctcagatata ttcagtcact ggttttagag ctagaaatag     1140

caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt     1200

tggccggcat ggtcccagcc tcctcgctgg cgccggctgg gcaacatgct tcggcatggc     1260

gaatgggact aaaatgcgct aaactgggct tgactcaggg agggatcatg gactagccaa     1320

ttgggcgtgc acagcgcgac tttggagctg gttctggctc gcatgacttg tttcgtgctg     1380

cgggggattc cgttcggacc tgacatttta aaaataaaaa atggaaacat cttgaaagac     1440

aaaaatgagt ttcagtagtg gtctacagac cgtagttttg ttcctattca cagtgaaaat     1500

aaggcgctgc aattgctacg ttcataaatc gagtattgtt gtgctccgaa gcgccagtcc     1560

ccatgttccg caccctcggg aagaagacgg ctgaccacgc aacttgcact gtccgattct     1620

ttgactg                                                               1627


<210>  86
<211>  4081
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SGIC DNA fragment II A

<400>  86
ctgcgacagc ggattgggcg gagaagaaga caacccttca gatatattca ggtgcttttc       60

cctcacatgt tttgccgcac cagccatccc actatcaaaa agcgatgatg tttgagattg      120

tcgggtgtcc acatctttta gtgtgaatcg ctagtagaat ttgggatatt attgagcatc      180

atcccatgat agcgagtaca agccccgagt aaataccaac attgctatgc tgctgtgctg      240

ctatctagtt tgctacgttg gtcgttgacc tcacagggat ttccaccaaa aagtggaccg      300

ggcgggcgcc actcggccgt gccacagcag cctgagagcg gacaaataac aacagccgcc      360

tgccgcgggg ttcggttgca aacatgacca acaggccagg ccatcatcaa cccaccgctg      420

cgttgatgcc caggatttca gtccaataat ccacaattta ccaacggata gagctaggtg      480

aattagatag acaggagggc cagagggagg ggaccgagat gaaaaatttt cgatgaaaga      540

gtggtcaagg tggggtcgta gttcggcgct ccgagggcga ggaaccaagg aaaggcgagg      600

aaaggacagg ctgatcgcgc tgcgttgctg ggctgcaagc gtgtccagtt gagtctggaa      660

aaggctccgc cgtgaagatt ctgcgttggt cccgcacctg cgcggtgggg gcattacccc      720

tccatgtcca atgatttcaa gtcaaagcca agggttgaag cccgcccgct tagtcgcctt      780

ctcgcttgac ccctccatat aagtatttcc cctcctcccc ctcccacaaa tttttccttt      840

ccctttcctc cctcgtccgc ttcagtacgt atatcttccc cccctctctc ttccttctca      900

ctcttctctc cttctttctt gattcatcct ctctctaact gacttctttg ctcagcacct      960

ctacgcgttc tggccgtagt atctgagcaa tttttctaca gactttttct atctaattcc     1020

aaaaaagaac ttcgagttca ttcaccaccg tcaaaatgat ctgactgatg agtccgtgag     1080

gacgaaacga gtaagctcgt ctcagatata ttcagtcact ggttttagag ctagaaatag     1140

caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt     1200

tggccggcat ggtcccagcc tcctcgctgg cgccggctgg gcaacatgct tcggcatggc     1260

gaatgggact aaaatgcgct aaactgggct tgactcaggg agggatcatg gactagccaa     1320

ttgggcgtgc acagcgcgac tttggagctg gttctggctc gcatgacttg tttcgtgctg     1380

cgggggattc cgttcggacc tgacatttta aaaataaaaa atggaaacat cttgaaagac     1440

aaaaatgagt ttcagtagtg gtctacagac cgtagttttg ttcctattca cagtgaaaat     1500

aaggcgctgc aattgctacg ttcataaatc gagtattgtt gtgctccgaa gcgccagtcc     1560

ccatgttccg caccctcaaa gccaaagttc gcgttccgac cttgcctccc aaatccgagt     1620

tgcgattaat ggctctgtac agtgaccggt gactctttct ggcatgcgga gagacggacg     1680

gacgcagaga gaagggctga gtaataagcg ccactgcgcc agacagctct ggcggctctg     1740

aggtgcagtg gatgattatt aatccgggac cggccgcccc tccgccccga agtggaaagg     1800

ctggtgtgcc cctcgttgac caagaatcta ttgcatcatc ggagaatatg gagcttcatc     1860

gaatcaccgg cagtaagcga aggagaatgt gaagccaggg gtgtatagcc gtcggcgaaa     1920

tagcatgcca ttaacctagg tacagaagtc caattgcttc cgatctggta aaagattcac     1980

gagatagtac cttctccgaa gtaggtagag cgagtacccg gcgcgtaagc tccctaattg     2040

gcccatccgg catctgtagg gcgtccaaat atcgtgcctc tcctgctttg cccggtgtat     2100

gaaaccggaa aggccgctca ggagctggcc agcggcgcag accgggaaca caagctggca     2160

gtcgacccat ccggtgctct gcactcgacc tgctgaggtc cctcagtccc tggtaggcag     2220

ctttgccccg tctgtccgcc cggtgtgtcg gcggggttga caaggtcgtt gcgtcagtcc     2280

aacatttgtt gccatatttt cctgctctcc ccaccagctg ctcttttctt ttctctttct     2340

tttcccatct tcagtatatt catcttccca tccaagaacc tttatttccc ctaagtaagt     2400

actttgctac atccatactc catccttccc atcccttatt cctttgaacc tttcagttcg     2460

agctttccca cttcatcgca gcttgactaa cagctacccc gcttgagcag acatcacagg     2520

atcccaccgt caaaatgcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa     2580

agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca     2640

gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct     2700

acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc     2760

ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg     2820

tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg     2880

ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac     2940

cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc     3000

atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc     3060

tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg     3120

atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga     3180

gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt     3240

ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag     3300

gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct     3360

tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc     3420

gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga     3480

ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga     3540

gggcaaagga ataaacccgg gaggctcttc atgacgagcc aatgcatctt ttgtatgtag     3600

cttcaaccga ctccgtcttc acttcttcgc ccgcactgcc taccgtttgt accatctgac     3660

tcatataaat gtctagcccc tacctacact atacctaagg gagagaagcg tagagtgatt     3720

aacgtacggg cctatagtac cccgatctct agatagaaca tttagtagag attaggatgc     3780

ctaactaatt taacttgagc attgtcccgt tcatattgat tttcagtcca ttatacactc     3840

ttaatcgttt cccggtagaa gcctgatata tacgaccata gggtgtggag aacagggctt     3900

cccgtctgct tggccgtact taagctatat attctacacg gccaatactc aatgtgccct     3960

tagcacctaa gcggcactct agggtaagtg cgggtgatat aggagagaag tcttaagact     4020

gaagacagga tgggaagaag acggctgacc acgcaacttg cactgtccga ttctttgact     4080

g                                                                     4081


<210>  87
<211>  3436
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SGIC DNA fragment II B

<400>  87
ctgcgacagc ggattgggcg gagaagaaga caacccttca gatatattca ggtgcttttc       60

cctcacatgt tttgccgcac cagccatccc actatcaaaa agcgatgatg tttgagattg      120

tcgggtgtcc acatctttta gtgtgaatcg ctagtagaat ttgggatatt attgagcatc      180

atcccatgat agcgagtaca agccccgagt aaataccaac attgctatgc tgctgtgctg      240

ctatctagtt tgctacgttg gtcgttgacc tcacagggat ttccaccaaa aagtggaccg      300

ggcgggcgcc actcggccgt gccacagcag cctgagagcg gacaaataac aacagccgcc      360

tgccgcgggg ttcggttgca aacatgacca acaggccagg ccatcatcaa cccaccgctg      420

cgttgatgcc caggatttca gtccaataat ccacaattta ccaacggata gagctaggtg      480

aattagatag acaggagggc cagagggagg ggaccgagat gaaaaatttt cgatgaaaga      540

gtggtcaagg tggggtcgta gttcggcgct ccgagggcga ggaaccaagg aaaggcgagg      600

aaaggacagg ctgatcgcgc tgcgttgctg ggctgcaagc gtgtccagtt gagtctggaa      660

aaggctccgc cgtgaagatt ctgcgttggt cccgcacctg cgcggtgggg gcattacccc      720

tccatgtcca atgatttcaa gtcaaagcca agggttgaag cccgcccgct tagtcgcctt      780

ctcgcttgac ccctccatat aagtatttcc cctcctcccc ctcccacaaa tttttccttt      840

ccctttcctc cctcgtccgc ttcagtacgt atatcttccc cccctctctc ttccttctca      900

ctcttctctc cttctttctt gattcatcct ctctctaact gacttctttg ctcagcacct      960

ctacgcgttc tggccgtagt atctgagcaa tttttctaca gactttttct atctaattcc     1020

aaaaaagaac ttcgagttca ttcaccaccg tcaaaatgat ctgactgatg agtccgtgag     1080

gacgaaacga gtaagctcgt ctcagatata ttcagtcact ggttttagag ctagaaatag     1140

caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt     1200

tggccggcat ggtcccagcc tcctcgctgg cgccggctgg gcaacatgct tcggcatggc     1260

gaatgggact aaaatgcgct aaactgggct tgactcaggg agggatcatg gactagccaa     1320

ttgggcgtgc acagcgcgac tttggagctg gttctggctc gcatgacttg tttcgtgctg     1380

cgggggattc cgttcggacc tgacatttta aaaataaaaa atggaaacat cttgaaagac     1440

aaaaatgagt ttcagtagtg gtctacagac cgtagttttg ttcctattca cagtgaaaat     1500

aaggcgctgc aattgctacg ttcataaatc gagtattgtt gtgctccgaa gcgccagtcc     1560

ccatgttccg caccctcaaa gccaaagttc gcgttccgac cttgcctccc aaatccgagt     1620

tgcgattaat ggctctgtac agtgaccggt gactctttct ggcatgcgga gagacggacg     1680

gacgcagaga gaagggctga gtaataagcg ccactgcgcc agacagctct ggcggctctg     1740

aggtgcagtg gatgattatt aatccgggac cggccgcccc tccgccccga agtggaaagg     1800

ctggtgtgcc cctcgttgac caagaatcta ttgcatcatc ggagaatatg gagcttcatc     1860

gaatcaccgg cagtaagcga aggagaatgt gaagccaggg gtgtatagcc gtcggcgaaa     1920

tagcatgcca ttaacctagg tacagaagtc caattgcttc cgatctggta aaagattcac     1980

gagatagtac cttctccgaa gtaggtagag cgagtacccg gcgcgtaagc tccctaattg     2040

gcccatccgg catctgtagg gcgtccaaat atcgtgcctc tcctgctttg cccggtgtat     2100

gaaaccggaa aggccgctca ggagctggcc agcggcgcag accgggaaca caagctggca     2160

gtcgacccat ccggtgctct gcactcgacc tgctgaggtc cctcagtccc tggtaggcag     2220

ctttgccccg tctgtccgcc cggtgtgtcg gcggggttga caaggtcgtt gcgtcagtcc     2280

aacatttgtt gccatatttt cctgctctcc ccaccagctg ctcttttctt ttctctttct     2340

tttcccatct tcagtatatt catcttccca tccaagaacc tttatttccc ctaagtaagt     2400

actttgctac atccatactc catccttccc atcccttatt cctttgaacc tttcagttcg     2460

agctttccca cttcatcgca gcttgactaa cagctacccc gcttgagcag acatcacagg     2520

atcccaccgt caaaatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg     2580

tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg     2640

acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc     2700

aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg     2760

ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg     2820

agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg     2880

tgcacttcgt ggccgaggag caggactaaa cccgggaggc tcttcatgac gagccaatgc     2940

atcttttgta tgtagcttca accgactccg tcttcacttc ttcgcccgca ctgcctaccg     3000

tttgtaccat ctgactcata taaatgtcta gcccctacct acactatacc taagggagag     3060

aagcgtagag tgattaacgt acgggcctat agtaccccga tctctagata gaacatttag     3120

tagagattag gatgcctaac taatttaact tgagcattgt cccgttcata ttgattttca     3180

gtccattata cactcttaat cgtttcccgg tagaagcctg atatatacga ccatagggtg     3240

tggagaacag ggcttcccgt ctgcttggcc gtacttaagc tatatattct acacggccaa     3300

tactcaatgt gcccttagca cctaagcggc actctagggt aagtgcgggt gatataggag     3360

agaagtctta agactgaaga caggatggga agaagacggc tgaccacgca acttgcactg     3420

tccgattctt tgactg                                                     3436


<210>  88
<211>  1627
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SGIC DNA fragment III

<400>  88
ctgcgacagc ggattgggcg gagaagaaga caacccttca gatatattca ggtgcttttc       60

cctcacatgt tttgccgcac cagccatccc actatcaaaa agcgatgatg tttgagattg      120

tcgggtgtcc acatctttta gtgtgaatcg ctagtagaat ttgggatatt attgagcatc      180

atcccatgat agcgagtaca agccccgagt aaataccaac attgctatgc tgctgtgctg      240

ctatctagtt tgctacgttg gtcgttgacc tcacagggat ttccaccaaa aagtggaccg      300

ggcgggcgcc actcggccgt gccacagcag cctgagagcg gacaaataac aacagccgcc      360

tgccgcgggg ttcggttgca aacatgacca acaggccagg ccatcatcaa cccaccgctg      420

cgttgatgcc caggatttca gtccaataat ccacaattta ccaacggata gagctaggtg      480

aattagatag acaggagggc cagagggagg ggaccgagat gaaaaatttt cgatgaaaga      540

gtggtcaagg tggggtcgta gttcggcgct ccgagggcga ggaaccaagg aaaggcgagg      600

aaaggacagg ctgatcgcgc tgcgttgctg ggctgcaagc gtgtccagtt gagtctggaa      660

aaggctccgc cgtgaagatt ctgcgttggt cccgcacctg cgcggtgggg gcattacccc      720

tccatgtcca atgatttcaa gtcaaagcca agggttgaag cccgcccgct tagtcgcctt      780

ctcgcttgac ccctccatat aagtatttcc cctcctcccc ctcccacaaa tttttccttt      840

ccctttcctc cctcgtccgc ttcagtacgt atatcttccc cccctctctc ttccttctca      900

ctcttctctc cttctttctt gattcatcct ctctctaact gacttctttg ctcagcacct      960

ctacgcgttc tggccgtagt atctgagcaa tttttctaca gactttttct atctaattcc     1020

aaaaaagaac ttcgagttca ttcaccaccg tcaaaatgat ctgactgatg agtccgtgag     1080

gacgaaacga gtaagctcgt ctcagatata ttcagtcact ggttttagag ctagaaatag     1140

caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt     1200

tggccggcat ggtcccagcc tcctcgctgg cgccggctgg gcaacatgct tcggcatggc     1260

gaatgggact aaaatgcgct aaactgggct tgactcaggg agggatcatg gactagccaa     1320

ttgggcgtgc acagcgcgac tttggagctg gttctggctc gcatgacttg tttcgtgctg     1380

cgggggattc cgttcggacc tgacatttta aaaataaaaa atggaaacat cttgaaagac     1440

aaaaatgagt ttcagtagtg gtctacagac cgtagttttg ttcctattca cagtgaaaat     1500

aaggcgctgc aattgctacg ttcataaatc gagtattgtt gtgctccgaa gcgccagtcc     1560

ccatgttccg caccctcaaa gccaaagttc gcgttccgac cttgcctccc aaatccgagt     1620

tgcgatt                                                               1627


<210>  89
<211>  2504
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SGIC DNA fragment IV A

<400>  89
aaagccaaag ttcgcgttcc gaccttgcct cccaaatccg agttgcgatt aatggctctg       60

tacagtgacc ggtgactctt tctggcatgc ggagagacgg acggacgcag agagaagggc      120

tgagtaataa gcgccactgc gccagacagc tctggcggct ctgaggtgca gtggatgatt      180

attaatccgg gaccggccgc ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt      240

gaccaagaat ctattgcatc atcggagaat atggagcttc atcgaatcac cggcagtaag      300

cgaaggagaa tgtgaagcca ggggtgtata gccgtcggcg aaatagcatg ccattaacct      360

aggtacagaa gtccaattgc ttccgatctg gtaaaagatt cacgagatag taccttctcc      420

gaagtaggta gagcgagtac ccggcgcgta agctccctaa ttggcccatc cggcatctgt      480

agggcgtcca aatatcgtgc ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc      540

tcaggagctg gccagcggcg cagaccggga acacaagctg gcagtcgacc catccggtgc      600

tctgcactcg acctgctgag gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc      660

gcccggtgtg tcggcggggt tgacaaggtc gttgcgtcag tccaacattt gttgccatat      720

tttcctgctc tccccaccag ctgctctttt cttttctctt tcttttccca tcttcagtat      780

attcatcttc ccatccaaga acctttattt cccctaagta agtactttgc tacatccata      840

ctccatcctt cccatccctt attcctttga acctttcagt tcgagctttc ccacttcatc      900

gcagcttgac taacagctac cccgcttgag cagacatcac aggatcccac cgtcaaaatg      960

cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc     1020

gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg     1080

cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt     1140

tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc     1200

agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg     1260

cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct     1320

gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa     1380

tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa     1440

actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt     1500

tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat     1560

gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg     1620

gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag     1680

cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg     1740

gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc     1800

gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact     1860

gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa     1920

gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaataaacc     1980

cgggaggctc ttcatgacga gccaatgcat cttttgtatg tagcttcaac cgactccgtc     2040

ttcacttctt cgcccgcact gcctaccgtt tgtaccatct gactcatata aatgtctagc     2100

ccctacctac actataccta agggagagaa gcgtagagtg attaacgtac gggcctatag     2160

taccccgatc tctagataga acatttagta gagattagga tgcctaacta atttaacttg     2220

agcattgtcc cgttcatatt gattttcagt ccattataca ctcttaatcg tttcccggta     2280

gaagcctgat atatacgacc atagggtgtg gagaacaggg cttcccgtct gcttggccgt     2340

acttaagcta tatattctac acggccaata ctcaatgtgc ccttagcacc taagcggcac     2400

tctagggtaa gtgcgggtga tataggagag aagtcttaag actgaagaca ggatgggaag     2460

aagacggctg accacgcaac ttgcactgtc cgattctttg actg                      2504


<210>  90
<211>  1859
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SGIC DNA fragment IV B

<400>  90
aaagccaaag ttcgcgttcc gaccttgcct cccaaatccg agttgcgatt aatggctctg       60

tacagtgacc ggtgactctt tctggcatgc ggagagacgg acggacgcag agagaagggc      120

tgagtaataa gcgccactgc gccagacagc tctggcggct ctgaggtgca gtggatgatt      180

attaatccgg gaccggccgc ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt      240

gaccaagaat ctattgcatc atcggagaat atggagcttc atcgaatcac cggcagtaag      300

cgaaggagaa tgtgaagcca ggggtgtata gccgtcggcg aaatagcatg ccattaacct      360

aggtacagaa gtccaattgc ttccgatctg gtaaaagatt cacgagatag taccttctcc      420

gaagtaggta gagcgagtac ccggcgcgta agctccctaa ttggcccatc cggcatctgt      480

agggcgtcca aatatcgtgc ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc      540

tcaggagctg gccagcggcg cagaccggga acacaagctg gcagtcgacc catccggtgc      600

tctgcactcg acctgctgag gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc      660

gcccggtgtg tcggcggggt tgacaaggtc gttgcgtcag tccaacattt gttgccatat      720

tttcctgctc tccccaccag ctgctctttt cttttctctt tcttttccca tcttcagtat      780

attcatcttc ccatccaaga acctttattt cccctaagta agtactttgc tacatccata      840

ctccatcctt cccatccctt attcctttga acctttcagt tcgagctttc ccacttcatc      900

gcagcttgac taacagctac cccgcttgag cagacatcac aggatcccac cgtcaaaatg      960

gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag     1020

ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg     1080

gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac     1140

accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc     1200

gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg     1260

tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag     1320

gagcaggact aaacccggga ggctcttcat gacgagccaa tgcatctttt gtatgtagct     1380

tcaaccgact ccgtcttcac ttcttcgccc gcactgccta ccgtttgtac catctgactc     1440

atataaatgt ctagccccta cctacactat acctaaggga gagaagcgta gagtgattaa     1500

cgtacgggcc tatagtaccc cgatctctag atagaacatt tagtagagat taggatgcct     1560

aactaattta acttgagcat tgtcccgttc atattgattt tcagtccatt atacactctt     1620

aatcgtttcc cggtagaagc ctgatatata cgaccatagg gtgtggagaa cagggcttcc     1680

cgtctgcttg gccgtactta agctatatat tctacacggc caatactcaa tgtgccctta     1740

gcacctaagc ggcactctag ggtaagtgcg ggtgatatag gagagaagtc ttaagactga     1800

agacaggatg ggaagaagac ggctgaccac gcaacttgca ctgtccgatt ctttgactg      1859


<210>  91
<211>  388
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the gBlock that contains the sgRNA 
       expression cassette to target ORF1; i.e. ORF1_SGIC DNA before the
       genomic flanking regions are added to either 5' and 3' end

<400>  91
tctttgaaaa gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt       60

ttctttcgag tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt      120

agtgccctct tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt      180

caaaagattt tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga      240

aacttctccg cagtgaaaga taaatgatcc ctataccaat tcctatggtg ttttagagct      300

agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc      360

ggtggtgctt tttttgtttt ttatgtct                                         388


<210>  92
<211>  388
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the gBlock that contains the sgRNA 
       expression cassette to target ORF2; i.e. ORF2_SGIC DNA before the
       genomic flanking regions are added to either 5' and 3' end

<400>  92
tctttgaaaa gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt       60

ttctttcgag tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt      120

agtgccctct tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt      180

caaaagattt tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga      240

aacttctccg cagtgaaaga taaatgatcg atgagcgtgg taaccgattg ttttagagct      300

agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc      360

ggtggtgctt tttttgtttt ttatgtct                                         388


<210>  93
<211>  388
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the gBlock that contains the sgRNA 
       expression cassette to target ORF3; i.e. ORF3_SGIC DNA before the
       genomic flanking regions are added to either 5' and 3' end

<400>  93
tctttgaaaa gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt       60

ttctttcgag tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt      120

agtgccctct tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt      180

caaaagattt tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga      240

aacttctccg cagtgaaaga taaatgatcg acccaatgct ggccaccggg ttttagagct      300

agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc      360

ggtggtgctt tttttgtttt ttatgtct                                         388


<210>  94
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the guide sequence (genomic target 
       sequence) of ORF1

<400>  94
cctataccaa ttcctatggt                                                   20


<210>  95
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the guide sequence (genomic target 
       sequence) of ORF2

<400>  95
gatgagcgtg gtaaccgatt                                                   20


<210>  96
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the guide sequence (genomic target 
       sequence) of ORF3

<400>  96
gacccaatgc tggccaccgg                                                   20


<210>  97
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the forward primer to obtain ORF1 SGIC DNA
       sequence for integration

<400>  97
acgaagacgt ttatagacat aaataaagag gaaacgcatt ccgtggtaga tctttgaaaa       60

gataatgtat gattatg                                                      77


<210>  98
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the reverse primer to obtain ORF1 SGIC DNA
       sequence for integration

<400>  98
tcctgtcatt aagagttttt attttttatt ataatactca acacgtgact agacataaaa       60

aacaaaaaaa gcacc                                                        75


<210>  99
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the forward primer to obtain ORF2 SGIC DNA
       sequence for integration

<400>  99
ccagcgtata caatctcgat agttggtttc ccgttctttc cactcccgtc tctttgaaaa       60

gataatgtat gattatg                                                      77


<210>  100
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the reverse primer to obtain ORF2 SGIC DNA
       sequence for integration

<400>  100
gtttttataa cgttcgctgc actgggggcc aagcacaggg caagatgctt agacataaaa       60

aacaaaaaaa gcacc                                                        75


<210>  101
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the forward primer to obtain ORF3 SGIC DNA
       sequence for integration

<400>  101
gcggcttcag ccgttctgaa ccttcaagat ggtgttcggg tgtgatttat tctttgaaaa       60

gataatgtat gattatg                                                      77


<210>  102
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the reverse primer to obtain ORF3 SGIC DNA
       sequence for integration

<400>  102
attatccaaa caaagggtct ttcgttagca aacctagaaa tctgcaaaaa agacataaaa       60

aacaaaaaaa gcacc                                                        75


<210>  103
<211>  488
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of ORF1 SGIC DNA with genomic flanking 
       regions attached at both the 5' and 3' end to either side for 
       integration

<400>  103
acgaagacgt ttatagacat aaataaagag gaaacgcatt ccgtggtaga tctttgaaaa       60

gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt ttctttcgag      120

tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt agtgccctct      180

tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt caaaagattt      240

tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga aacttctccg      300

cagtgaaaga taaatgatcc ctataccaat tcctatggtg ttttagagct agaaatagca      360

agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtggtgctt      420

tttttgtttt ttatgtctag tcacgtgttg agtattataa taaaaaataa aaactcttaa      480

tgacagga                                                               488


<210>  104
<211>  488
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of ORF2 SGIC DNA with genomic flanking 
       regions attached at both the 5' and 3' end to either side for 
       integration

<400>  104
ccagcgtata caatctcgat agttggtttc ccgttctttc cactcccgtc tctttgaaaa       60

gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt ttctttcgag      120

tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt agtgccctct      180

tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt caaaagattt      240

tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga aacttctccg      300

cagtgaaaga taaatgatcg atgagcgtgg taaccgattg ttttagagct agaaatagca      360

agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtggtgctt      420

tttttgtttt ttatgtctaa gcatcttgcc ctgtgcttgg cccccagtgc agcgaacgtt      480

ataaaaac                                                               488


<210>  105
<211>  488
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of ORF3 SGIC DNA with genomic flanking 
       regions attached at both the 5' and 3' end to either side for 
       integration

<400>  105
gcggcttcag ccgttctgaa ccttcaagat ggtgttcggg tgtgatttat tctttgaaaa       60

gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt ttctttcgag      120

tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt agtgccctct      180

tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt caaaagattt      240

tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga aacttctccg      300

cagtgaaaga taaatgatcg acccaatgct ggccaccggg ttttagagct agaaatagca      360

agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtggtgctt      420

tttttgtttt ttatgtcttt tttgcagatt tctaggtttg ctaacgaaag accctttgtt      480

tggataat                                                               488


<210>  106
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of forward primer to confirm knock out of 
       ORF1 by integration of ORF1 SGIC DNA

<400>  106
tccctatacc aattcctatg gtgt                                              24


<210>  107
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of reverse primer to confirm knock out of 
       ORF1 by integration of ORF1 SGIC DNA

<400>  107
tggttcagtt cacagggctt                                                   20


<210>  108
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of forward primer to confirm knock out of 
       ORF2 by integration of ORF2 SGIC DNA

<400>  108
atcgatgagc gtggtaaccg                                                   20


<210>  109
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of reverse primer to confirm knock out of 
       ORF2 by integration of ORF2 SGIC DNA

<400>  109
cgcatgcacg aaaaagggaa                                                   20


<210>  110
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of forward primer to confirm knock out of 
       ORF3 by integration of ORF3 SGIC DNA

<400>  110
tgatcgaccc aatgctggc                                                    19


<210>  111
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of reverse primer to confirm knock out of 
       ORF3 by integration of ORF3 SGIC DNA

<400>  111
tcttcttgaa ccatgaaccc gt                                                22


