                         SEQUENCE LISTING

<110>  Danmarks Tekniske Universitet
       European Molecular Biology Laboratory
 
<120>  A method for improved glycerol utilization in yeast

<130>  P2216PC00

<150>  EP17150378
<151>  2017-01-05

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  2130
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(2130)
<223>  GUT1 wild-type gene encoding wild-type Gut1p

<400>  1
atg ttt ccc tct ctc ttc cga ctt gta gta ttc tcc aaa cgt tac ata         48
Met Phe Pro Ser Leu Phe Arg Leu Val Val Phe Ser Lys Arg Tyr Ile           
1               5                   10                  15                

ttc cga tca agc cag cgc ctt tac act agt tta aaa caa gaa cag agc         96
Phe Arg Ser Ser Gln Arg Leu Tyr Thr Ser Leu Lys Gln Glu Gln Ser           
            20                  25                  30                    

cgt atg tcc aaa ata atg gaa gat tta cga agt gac tac gtc ccg ctt        144
Arg Met Ser Lys Ile Met Glu Asp Leu Arg Ser Asp Tyr Val Pro Leu           
        35                  40                  45                        

atc gcc agt att gat gta gga acg acc tca tcc aga tgc att ctg ttc        192
Ile Ala Ser Ile Asp Val Gly Thr Thr Ser Ser Arg Cys Ile Leu Phe           
    50                  55                  60                            

aac aga tgg ggc cag gac gtt tca aaa cac caa att gaa tat tca act        240
Asn Arg Trp Gly Gln Asp Val Ser Lys His Gln Ile Glu Tyr Ser Thr           
65                  70                  75                  80            

tca gca tcg aag ggc aag att ggg gtg tct ggc cta agg aga ccc tct        288
Ser Ala Ser Lys Gly Lys Ile Gly Val Ser Gly Leu Arg Arg Pro Ser           
                85                  90                  95                

aca gcc cca gct cgt gaa aca cca aac gcc ggt gac atc aaa acc agc        336
Thr Ala Pro Ala Arg Glu Thr Pro Asn Ala Gly Asp Ile Lys Thr Ser           
            100                 105                 110                   

gga aag ccc atc ttt tct gca gaa ggc tat gcc att caa gaa acc aaa        384
Gly Lys Pro Ile Phe Ser Ala Glu Gly Tyr Ala Ile Gln Glu Thr Lys           
        115                 120                 125                       

ttc cta aaa atc gag gaa ttg gac ttg gac ttc cat aac gaa ccc acg        432
Phe Leu Lys Ile Glu Glu Leu Asp Leu Asp Phe His Asn Glu Pro Thr           
    130                 135                 140                           

ttg aag ttc ccc aaa ccg ggt tgg gtt gag tgc cat ccg cag aaa tta        480
Leu Lys Phe Pro Lys Pro Gly Trp Val Glu Cys His Pro Gln Lys Leu           
145                 150                 155                 160           

ctg gtg aac gtc gtc caa tgc ctt gcc tca agt ttg ctc tct ctg cag        528
Leu Val Asn Val Val Gln Cys Leu Ala Ser Ser Leu Leu Ser Leu Gln           
                165                 170                 175               

act atc aac agc gaa cgt gta gca aac ggt ctc cca cct tac aag gta        576
Thr Ile Asn Ser Glu Arg Val Ala Asn Gly Leu Pro Pro Tyr Lys Val           
            180                 185                 190                   

ata tgc atg ggt ata gca aac atg aga gaa acc aca att ctg tgg tcc        624
Ile Cys Met Gly Ile Ala Asn Met Arg Glu Thr Thr Ile Leu Trp Ser           
        195                 200                 205                       

cgc cgc aca gga aaa cca att gtt aac tac ggt att gtt tgg aac gac        672
Arg Arg Thr Gly Lys Pro Ile Val Asn Tyr Gly Ile Val Trp Asn Asp           
    210                 215                 220                           

acc aga acg atc aaa atc gtt aga gac aaa tgg caa aac act agc gtc        720
Thr Arg Thr Ile Lys Ile Val Arg Asp Lys Trp Gln Asn Thr Ser Val           
225                 230                 235                 240           

gat agg caa ctg cag ctt aga cag aag act gga ttg cca ttg ctc tcc        768
Asp Arg Gln Leu Gln Leu Arg Gln Lys Thr Gly Leu Pro Leu Leu Ser           
                245                 250                 255               

acg tat ttc tcc tgt tcc aag ctg cgc tgg ttc ctc gac aat gag cct        816
Thr Tyr Phe Ser Cys Ser Lys Leu Arg Trp Phe Leu Asp Asn Glu Pro           
            260                 265                 270                   

ctg tgt acc aag gcg tat gag gag aac gac ctg atg ttc ggc act gtg        864
Leu Cys Thr Lys Ala Tyr Glu Glu Asn Asp Leu Met Phe Gly Thr Val           
        275                 280                 285                       

gac aca tgg ctg att tac caa tta act aaa caa aag gcg ttc gtt tct        912
Asp Thr Trp Leu Ile Tyr Gln Leu Thr Lys Gln Lys Ala Phe Val Ser           
    290                 295                 300                           

gac gta acc aac gct tcc aga act gga ttt atg aac ctc tcc act tta        960
Asp Val Thr Asn Ala Ser Arg Thr Gly Phe Met Asn Leu Ser Thr Leu           
305                 310                 315                 320           

aag tac gac aac gag ttg ctg gaa ttt tgg ggt att gac aag aac ctg       1008
Lys Tyr Asp Asn Glu Leu Leu Glu Phe Trp Gly Ile Asp Lys Asn Leu           
                325                 330                 335               

att cac atg ccc gaa att gtg tcc tca tct caa tac tac ggt gac ttt       1056
Ile His Met Pro Glu Ile Val Ser Ser Ser Gln Tyr Tyr Gly Asp Phe           
            340                 345                 350                   

ggc att cct gat tgg ata atg gaa aag cta cac gat tcg cca aaa aca       1104
Gly Ile Pro Asp Trp Ile Met Glu Lys Leu His Asp Ser Pro Lys Thr           
        355                 360                 365                       

gta ctg cga gat cta gtc aag aga aac ctg ccc ata cag ggc tgt ctg       1152
Val Leu Arg Asp Leu Val Lys Arg Asn Leu Pro Ile Gln Gly Cys Leu           
    370                 375                 380                           

ggc gac caa agc gca tcc atg gtg ggg caa ctc gct tac aaa ccc ggt       1200
Gly Asp Gln Ser Ala Ser Met Val Gly Gln Leu Ala Tyr Lys Pro Gly           
385                 390                 395                 400           

gct gca aaa tgt act tat ggt acc ggt tgc ttt tta ctg tac aat acg       1248
Ala Ala Lys Cys Thr Tyr Gly Thr Gly Cys Phe Leu Leu Tyr Asn Thr           
                405                 410                 415               

ggg acc aaa aaa ttg atc tcc caa cat ggc gca ctg acg act cta gca       1296
Gly Thr Lys Lys Leu Ile Ser Gln His Gly Ala Leu Thr Thr Leu Ala           
            420                 425                 430                   

ttt tgg ttc cca cat ttg caa gag tac ggt ggc caa aaa cca gaa ttg       1344
Phe Trp Phe Pro His Leu Gln Glu Tyr Gly Gly Gln Lys Pro Glu Leu           
        435                 440                 445                       

agc aag cca cat ttt gca tta gag ggt tcc gtc gct gtg gct ggt gct       1392
Ser Lys Pro His Phe Ala Leu Glu Gly Ser Val Ala Val Ala Gly Ala           
    450                 455                 460                           

gtg gtc caa tgg cta cgt gat aat tta cga ttg atc gat aaa tca gag       1440
Val Val Gln Trp Leu Arg Asp Asn Leu Arg Leu Ile Asp Lys Ser Glu           
465                 470                 475                 480           

gat gtc gga ccg att gca tct acg gtt cct gat tct ggt ggc gta gtt       1488
Asp Val Gly Pro Ile Ala Ser Thr Val Pro Asp Ser Gly Gly Val Val           
                485                 490                 495               

ttc gtc ccc gca ttt agt ggc cta ttc gct ccc tat tgg gac cca gat       1536
Phe Val Pro Ala Phe Ser Gly Leu Phe Ala Pro Tyr Trp Asp Pro Asp           
            500                 505                 510                   

gcc aga gcc acc ata atg ggg atg tct caa ttc act act gcc tcc cac       1584
Ala Arg Ala Thr Ile Met Gly Met Ser Gln Phe Thr Thr Ala Ser His           
        515                 520                 525                       

atc gcc aga gct gcc gtg gaa ggt gtt tgc ttt caa gcc agg gct atc       1632
Ile Ala Arg Ala Ala Val Glu Gly Val Cys Phe Gln Ala Arg Ala Ile           
    530                 535                 540                           

ttg aag gca atg agt tct gac gcg ttt ggt gaa ggt tcc aaa gac agg       1680
Leu Lys Ala Met Ser Ser Asp Ala Phe Gly Glu Gly Ser Lys Asp Arg           
545                 550                 555                 560           

gac ttt tta gag gaa att tcc gac gtc aca tat gaa aag tcg ccc ctg       1728
Asp Phe Leu Glu Glu Ile Ser Asp Val Thr Tyr Glu Lys Ser Pro Leu           
                565                 570                 575               

tcg gtt ctg gca gtg gat ggc ggg atg tcg agg tct aat gaa gtc atg       1776
Ser Val Leu Ala Val Asp Gly Gly Met Ser Arg Ser Asn Glu Val Met           
            580                 585                 590                   

caa att caa gcc gat atc tta ggt ccc tgt gtc aaa gtc aga agg tct       1824
Gln Ile Gln Ala Asp Ile Leu Gly Pro Cys Val Lys Val Arg Arg Ser           
        595                 600                 605                       

ccg aca gcg gaa tgt acc gca ttg ggg gca gcc att gca gcc aat atg       1872
Pro Thr Ala Glu Cys Thr Ala Leu Gly Ala Ala Ile Ala Ala Asn Met           
    610                 615                 620                           

gct ttc aag gat gtg aac gag cgc cca tta tgg aag gac cta cac gat       1920
Ala Phe Lys Asp Val Asn Glu Arg Pro Leu Trp Lys Asp Leu His Asp           
625                 630                 635                 640           

gtt aag aaa tgg gtc ttt tac aat gga atg gag aaa aac gaa caa ata       1968
Val Lys Lys Trp Val Phe Tyr Asn Gly Met Glu Lys Asn Glu Gln Ile           
                645                 650                 655               

tca cca gag gct cat cca aac ctt aag ata ttc aga agt gaa tcc gac       2016
Ser Pro Glu Ala His Pro Asn Leu Lys Ile Phe Arg Ser Glu Ser Asp           
            660                 665                 670                   

gat gct gaa agg aga aag cat tgg aag tat tgg gaa gtt gcc gtg gaa       2064
Asp Ala Glu Arg Arg Lys His Trp Lys Tyr Trp Glu Val Ala Val Glu           
        675                 680                 685                       

aga tcc aaa ggt tgg ctg aag gac ata gaa ggt gaa cac gaa cag gtt       2112
Arg Ser Lys Gly Trp Leu Lys Asp Ile Glu Gly Glu His Glu Gln Val           
    690                 695                 700                           

cta gaa aac ttc caa taa                                               2130
Leu Glu Asn Phe Gln                                                       
705                                                                       


<210>  2
<211>  709
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  2

Met Phe Pro Ser Leu Phe Arg Leu Val Val Phe Ser Lys Arg Tyr Ile 
1               5                   10                  15      


Phe Arg Ser Ser Gln Arg Leu Tyr Thr Ser Leu Lys Gln Glu Gln Ser 
            20                  25                  30          


Arg Met Ser Lys Ile Met Glu Asp Leu Arg Ser Asp Tyr Val Pro Leu 
        35                  40                  45              


Ile Ala Ser Ile Asp Val Gly Thr Thr Ser Ser Arg Cys Ile Leu Phe 
    50                  55                  60                  


Asn Arg Trp Gly Gln Asp Val Ser Lys His Gln Ile Glu Tyr Ser Thr 
65                  70                  75                  80  


Ser Ala Ser Lys Gly Lys Ile Gly Val Ser Gly Leu Arg Arg Pro Ser 
                85                  90                  95      


Thr Ala Pro Ala Arg Glu Thr Pro Asn Ala Gly Asp Ile Lys Thr Ser 
            100                 105                 110         


Gly Lys Pro Ile Phe Ser Ala Glu Gly Tyr Ala Ile Gln Glu Thr Lys 
        115                 120                 125             


Phe Leu Lys Ile Glu Glu Leu Asp Leu Asp Phe His Asn Glu Pro Thr 
    130                 135                 140                 


Leu Lys Phe Pro Lys Pro Gly Trp Val Glu Cys His Pro Gln Lys Leu 
145                 150                 155                 160 


Leu Val Asn Val Val Gln Cys Leu Ala Ser Ser Leu Leu Ser Leu Gln 
                165                 170                 175     


Thr Ile Asn Ser Glu Arg Val Ala Asn Gly Leu Pro Pro Tyr Lys Val 
            180                 185                 190         


Ile Cys Met Gly Ile Ala Asn Met Arg Glu Thr Thr Ile Leu Trp Ser 
        195                 200                 205             


Arg Arg Thr Gly Lys Pro Ile Val Asn Tyr Gly Ile Val Trp Asn Asp 
    210                 215                 220                 


Thr Arg Thr Ile Lys Ile Val Arg Asp Lys Trp Gln Asn Thr Ser Val 
225                 230                 235                 240 


Asp Arg Gln Leu Gln Leu Arg Gln Lys Thr Gly Leu Pro Leu Leu Ser 
                245                 250                 255     


Thr Tyr Phe Ser Cys Ser Lys Leu Arg Trp Phe Leu Asp Asn Glu Pro 
            260                 265                 270         


Leu Cys Thr Lys Ala Tyr Glu Glu Asn Asp Leu Met Phe Gly Thr Val 
        275                 280                 285             


Asp Thr Trp Leu Ile Tyr Gln Leu Thr Lys Gln Lys Ala Phe Val Ser 
    290                 295                 300                 


Asp Val Thr Asn Ala Ser Arg Thr Gly Phe Met Asn Leu Ser Thr Leu 
305                 310                 315                 320 


Lys Tyr Asp Asn Glu Leu Leu Glu Phe Trp Gly Ile Asp Lys Asn Leu 
                325                 330                 335     


Ile His Met Pro Glu Ile Val Ser Ser Ser Gln Tyr Tyr Gly Asp Phe 
            340                 345                 350         


Gly Ile Pro Asp Trp Ile Met Glu Lys Leu His Asp Ser Pro Lys Thr 
        355                 360                 365             


Val Leu Arg Asp Leu Val Lys Arg Asn Leu Pro Ile Gln Gly Cys Leu 
    370                 375                 380                 


Gly Asp Gln Ser Ala Ser Met Val Gly Gln Leu Ala Tyr Lys Pro Gly 
385                 390                 395                 400 


Ala Ala Lys Cys Thr Tyr Gly Thr Gly Cys Phe Leu Leu Tyr Asn Thr 
                405                 410                 415     


Gly Thr Lys Lys Leu Ile Ser Gln His Gly Ala Leu Thr Thr Leu Ala 
            420                 425                 430         


Phe Trp Phe Pro His Leu Gln Glu Tyr Gly Gly Gln Lys Pro Glu Leu 
        435                 440                 445             


Ser Lys Pro His Phe Ala Leu Glu Gly Ser Val Ala Val Ala Gly Ala 
    450                 455                 460                 


Val Val Gln Trp Leu Arg Asp Asn Leu Arg Leu Ile Asp Lys Ser Glu 
465                 470                 475                 480 


Asp Val Gly Pro Ile Ala Ser Thr Val Pro Asp Ser Gly Gly Val Val 
                485                 490                 495     


Phe Val Pro Ala Phe Ser Gly Leu Phe Ala Pro Tyr Trp Asp Pro Asp 
            500                 505                 510         


Ala Arg Ala Thr Ile Met Gly Met Ser Gln Phe Thr Thr Ala Ser His 
        515                 520                 525             


Ile Ala Arg Ala Ala Val Glu Gly Val Cys Phe Gln Ala Arg Ala Ile 
    530                 535                 540                 


Leu Lys Ala Met Ser Ser Asp Ala Phe Gly Glu Gly Ser Lys Asp Arg 
545                 550                 555                 560 


Asp Phe Leu Glu Glu Ile Ser Asp Val Thr Tyr Glu Lys Ser Pro Leu 
                565                 570                 575     


Ser Val Leu Ala Val Asp Gly Gly Met Ser Arg Ser Asn Glu Val Met 
            580                 585                 590         


Gln Ile Gln Ala Asp Ile Leu Gly Pro Cys Val Lys Val Arg Arg Ser 
        595                 600                 605             


Pro Thr Ala Glu Cys Thr Ala Leu Gly Ala Ala Ile Ala Ala Asn Met 
    610                 615                 620                 


Ala Phe Lys Asp Val Asn Glu Arg Pro Leu Trp Lys Asp Leu His Asp 
625                 630                 635                 640 


Val Lys Lys Trp Val Phe Tyr Asn Gly Met Glu Lys Asn Glu Gln Ile 
                645                 650                 655     


Ser Pro Glu Ala His Pro Asn Leu Lys Ile Phe Arg Ser Glu Ser Asp 
            660                 665                 670         


Asp Ala Glu Arg Arg Lys His Trp Lys Tyr Trp Glu Val Ala Val Glu 
        675                 680                 685             


Arg Ser Lys Gly Trp Leu Lys Asp Ile Glu Gly Glu His Glu Gln Val 
    690                 695                 700                 


Leu Glu Asn Phe Gln 
705                 


<210>  3
<211>  2130
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(2130)
<223>  Mutant GUT1 gene (G1711C) encoding mutant Gut1-1 protein (E572Q)

<400>  3
atg ttt ccc tct ctc ttc cga ctt gta gta ttc tcc aaa cgt tac ata         48
Met Phe Pro Ser Leu Phe Arg Leu Val Val Phe Ser Lys Arg Tyr Ile           
1               5                   10                  15                

ttc cga tca agc cag cgc ctt tac act agt tta aaa caa gaa cag agc         96
Phe Arg Ser Ser Gln Arg Leu Tyr Thr Ser Leu Lys Gln Glu Gln Ser           
            20                  25                  30                    

cgt atg tcc aaa ata atg gaa gat tta cga agt gac tac gtc ccg ctt        144
Arg Met Ser Lys Ile Met Glu Asp Leu Arg Ser Asp Tyr Val Pro Leu           
        35                  40                  45                        

atc gcc agt att gat gta gga acg acc tca tcc aga tgc att ctg ttc        192
Ile Ala Ser Ile Asp Val Gly Thr Thr Ser Ser Arg Cys Ile Leu Phe           
    50                  55                  60                            

aac aga tgg ggc cag gac gtt tca aaa cac caa att gaa tat tca act        240
Asn Arg Trp Gly Gln Asp Val Ser Lys His Gln Ile Glu Tyr Ser Thr           
65                  70                  75                  80            

tca gca tcg aag ggc aag att ggg gtg tct ggc cta agg aga ccc tct        288
Ser Ala Ser Lys Gly Lys Ile Gly Val Ser Gly Leu Arg Arg Pro Ser           
                85                  90                  95                

aca gcc cca gct cgt gaa aca cca aac gcc ggt gac atc aaa acc agc        336
Thr Ala Pro Ala Arg Glu Thr Pro Asn Ala Gly Asp Ile Lys Thr Ser           
            100                 105                 110                   

gga aag ccc atc ttt tct gca gaa ggc tat gcc att caa gaa acc aaa        384
Gly Lys Pro Ile Phe Ser Ala Glu Gly Tyr Ala Ile Gln Glu Thr Lys           
        115                 120                 125                       

ttc cta aaa atc gag gaa ttg gac ttg gac ttc cat aac gaa ccc acg        432
Phe Leu Lys Ile Glu Glu Leu Asp Leu Asp Phe His Asn Glu Pro Thr           
    130                 135                 140                           

ttg aag ttc ccc aaa ccg ggt tgg gtt gag tgc cat ccg cag aaa tta        480
Leu Lys Phe Pro Lys Pro Gly Trp Val Glu Cys His Pro Gln Lys Leu           
145                 150                 155                 160           

ctg gtg aac gtc gtc caa tgc ctt gcc tca agt ttg ctc tct ctg cag        528
Leu Val Asn Val Val Gln Cys Leu Ala Ser Ser Leu Leu Ser Leu Gln           
                165                 170                 175               

act atc aac agc gaa cgt gta gca aac ggt ctc cca cct tac aag gta        576
Thr Ile Asn Ser Glu Arg Val Ala Asn Gly Leu Pro Pro Tyr Lys Val           
            180                 185                 190                   

ata tgc atg ggt ata gca aac atg aga gaa acc aca att ctg tgg tcc        624
Ile Cys Met Gly Ile Ala Asn Met Arg Glu Thr Thr Ile Leu Trp Ser           
        195                 200                 205                       

cgc cgc aca gga aaa cca att gtt aac tac ggt att gtt tgg aac gac        672
Arg Arg Thr Gly Lys Pro Ile Val Asn Tyr Gly Ile Val Trp Asn Asp           
    210                 215                 220                           

acc aga acg atc aaa atc gtt aga gac aaa tgg caa aac act agc gtc        720
Thr Arg Thr Ile Lys Ile Val Arg Asp Lys Trp Gln Asn Thr Ser Val           
225                 230                 235                 240           

gat agg caa ctg cag ctt aga cag aag act gga ttg cca ttg ctc tcc        768
Asp Arg Gln Leu Gln Leu Arg Gln Lys Thr Gly Leu Pro Leu Leu Ser           
                245                 250                 255               

acg tat ttc tcc tgt tcc aag ctg cgc tgg ttc ctc gac aat gag cct        816
Thr Tyr Phe Ser Cys Ser Lys Leu Arg Trp Phe Leu Asp Asn Glu Pro           
            260                 265                 270                   

ctg tgt acc aag gcg tat gag gag aac gac ctg atg ttc ggc act gtg        864
Leu Cys Thr Lys Ala Tyr Glu Glu Asn Asp Leu Met Phe Gly Thr Val           
        275                 280                 285                       

gac aca tgg ctg att tac caa tta act aaa caa aag gcg ttc gtt tct        912
Asp Thr Trp Leu Ile Tyr Gln Leu Thr Lys Gln Lys Ala Phe Val Ser           
    290                 295                 300                           

gac gta acc aac gct tcc aga act gga ttt atg aac ctc tcc act tta        960
Asp Val Thr Asn Ala Ser Arg Thr Gly Phe Met Asn Leu Ser Thr Leu           
305                 310                 315                 320           

aag tac gac aac gag ttg ctg gaa ttt tgg ggt att gac aag aac ctg       1008
Lys Tyr Asp Asn Glu Leu Leu Glu Phe Trp Gly Ile Asp Lys Asn Leu           
                325                 330                 335               

att cac atg ccc gaa att gtg tcc tca tct caa tac tac ggt gac ttt       1056
Ile His Met Pro Glu Ile Val Ser Ser Ser Gln Tyr Tyr Gly Asp Phe           
            340                 345                 350                   

ggc att cct gat tgg ata atg gaa aag cta cac gat tcg cca aaa aca       1104
Gly Ile Pro Asp Trp Ile Met Glu Lys Leu His Asp Ser Pro Lys Thr           
        355                 360                 365                       

gta ctg cga gat cta gtc aag aga aac ctg ccc ata cag ggc tgt ctg       1152
Val Leu Arg Asp Leu Val Lys Arg Asn Leu Pro Ile Gln Gly Cys Leu           
    370                 375                 380                           

ggc gac caa agc gca tcc atg gtg ggg caa ctc gct tac aaa ccc ggt       1200
Gly Asp Gln Ser Ala Ser Met Val Gly Gln Leu Ala Tyr Lys Pro Gly           
385                 390                 395                 400           

gct gca aaa tgt act tat ggt acc ggt tgc ttt tta ctg tac aat acg       1248
Ala Ala Lys Cys Thr Tyr Gly Thr Gly Cys Phe Leu Leu Tyr Asn Thr           
                405                 410                 415               

ggg acc aaa aaa ttg atc tcc caa cat ggc gca ctg acg act cta gca       1296
Gly Thr Lys Lys Leu Ile Ser Gln His Gly Ala Leu Thr Thr Leu Ala           
            420                 425                 430                   

ttt tgg ttc cca cat ttg caa gag tac ggt ggc caa aaa cca gaa ttg       1344
Phe Trp Phe Pro His Leu Gln Glu Tyr Gly Gly Gln Lys Pro Glu Leu           
        435                 440                 445                       

agc aag cca cat ttt gca tta gag ggt tcc gtc gct gtg gct ggt gct       1392
Ser Lys Pro His Phe Ala Leu Glu Gly Ser Val Ala Val Ala Gly Ala           
    450                 455                 460                           

gtg gtc caa tgg cta cgt gat aat tta cga ttg atc gat aaa tca gag       1440
Val Val Gln Trp Leu Arg Asp Asn Leu Arg Leu Ile Asp Lys Ser Glu           
465                 470                 475                 480           

gat gtc gga ccg att gca tct acg gtt cct gat tct ggt ggc gta gtt       1488
Asp Val Gly Pro Ile Ala Ser Thr Val Pro Asp Ser Gly Gly Val Val           
                485                 490                 495               

ttc gtc ccc gca ttt agt ggc cta ttc gct ccc tat tgg gac cca gat       1536
Phe Val Pro Ala Phe Ser Gly Leu Phe Ala Pro Tyr Trp Asp Pro Asp           
            500                 505                 510                   

gcc aga gcc acc ata atg ggg atg tct caa ttc act act gcc tcc cac       1584
Ala Arg Ala Thr Ile Met Gly Met Ser Gln Phe Thr Thr Ala Ser His           
        515                 520                 525                       

atc gcc aga gct gcc gtg gaa ggt gtt tgc ttt caa gcc agg gct atc       1632
Ile Ala Arg Ala Ala Val Glu Gly Val Cys Phe Gln Ala Arg Ala Ile           
    530                 535                 540                           

ttg aag gca atg agt tct gac gcg ttt ggt gaa ggt tcc aaa gac agg       1680
Leu Lys Ala Met Ser Ser Asp Ala Phe Gly Glu Gly Ser Lys Asp Arg           
545                 550                 555                 560           

gac ttt tta gag gaa att tcc gac gtc aca tat caa aag tcg ccc ctg       1728
Asp Phe Leu Glu Glu Ile Ser Asp Val Thr Tyr Gln Lys Ser Pro Leu           
                565                 570                 575               

tcg gtt ctg gca gtg gat ggc ggg atg tcg agg tct aat gaa gtc atg       1776
Ser Val Leu Ala Val Asp Gly Gly Met Ser Arg Ser Asn Glu Val Met           
            580                 585                 590                   

caa att caa gcc gat atc tta ggt ccc tgt gtc aaa gtc aga agg tct       1824
Gln Ile Gln Ala Asp Ile Leu Gly Pro Cys Val Lys Val Arg Arg Ser           
        595                 600                 605                       

ccg aca gcg gaa tgt acc gca ttg ggg gca gcc att gca gcc aat atg       1872
Pro Thr Ala Glu Cys Thr Ala Leu Gly Ala Ala Ile Ala Ala Asn Met           
    610                 615                 620                           

gct ttc aag gat gtg aac gag cgc cca tta tgg aag gac cta cac gat       1920
Ala Phe Lys Asp Val Asn Glu Arg Pro Leu Trp Lys Asp Leu His Asp           
625                 630                 635                 640           

gtt aag aaa tgg gtc ttt tac aat gga atg gag aaa aac gaa caa ata       1968
Val Lys Lys Trp Val Phe Tyr Asn Gly Met Glu Lys Asn Glu Gln Ile           
                645                 650                 655               

tca cca gag gct cat cca aac ctt aag ata ttc aga agt gaa tcc gac       2016
Ser Pro Glu Ala His Pro Asn Leu Lys Ile Phe Arg Ser Glu Ser Asp           
            660                 665                 670                   

gat gct gaa agg aga aag cat tgg aag tat tgg gaa gtt gcc gtg gaa       2064
Asp Ala Glu Arg Arg Lys His Trp Lys Tyr Trp Glu Val Ala Val Glu           
        675                 680                 685                       

aga tcc aaa ggt tgg ctg aag gac ata gaa ggt gaa cac gaa cag gtt       2112
Arg Ser Lys Gly Trp Leu Lys Asp Ile Glu Gly Glu His Glu Gln Val           
    690                 695                 700                           

cta gaa aac ttc caa taa                                               2130
Leu Glu Asn Phe Gln                                                       
705                                                                       


<210>  4
<211>  709
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  4

Met Phe Pro Ser Leu Phe Arg Leu Val Val Phe Ser Lys Arg Tyr Ile 
1               5                   10                  15      


Phe Arg Ser Ser Gln Arg Leu Tyr Thr Ser Leu Lys Gln Glu Gln Ser 
            20                  25                  30          


Arg Met Ser Lys Ile Met Glu Asp Leu Arg Ser Asp Tyr Val Pro Leu 
        35                  40                  45              


Ile Ala Ser Ile Asp Val Gly Thr Thr Ser Ser Arg Cys Ile Leu Phe 
    50                  55                  60                  


Asn Arg Trp Gly Gln Asp Val Ser Lys His Gln Ile Glu Tyr Ser Thr 
65                  70                  75                  80  


Ser Ala Ser Lys Gly Lys Ile Gly Val Ser Gly Leu Arg Arg Pro Ser 
                85                  90                  95      


Thr Ala Pro Ala Arg Glu Thr Pro Asn Ala Gly Asp Ile Lys Thr Ser 
            100                 105                 110         


Gly Lys Pro Ile Phe Ser Ala Glu Gly Tyr Ala Ile Gln Glu Thr Lys 
        115                 120                 125             


Phe Leu Lys Ile Glu Glu Leu Asp Leu Asp Phe His Asn Glu Pro Thr 
    130                 135                 140                 


Leu Lys Phe Pro Lys Pro Gly Trp Val Glu Cys His Pro Gln Lys Leu 
145                 150                 155                 160 


Leu Val Asn Val Val Gln Cys Leu Ala Ser Ser Leu Leu Ser Leu Gln 
                165                 170                 175     


Thr Ile Asn Ser Glu Arg Val Ala Asn Gly Leu Pro Pro Tyr Lys Val 
            180                 185                 190         


Ile Cys Met Gly Ile Ala Asn Met Arg Glu Thr Thr Ile Leu Trp Ser 
        195                 200                 205             


Arg Arg Thr Gly Lys Pro Ile Val Asn Tyr Gly Ile Val Trp Asn Asp 
    210                 215                 220                 


Thr Arg Thr Ile Lys Ile Val Arg Asp Lys Trp Gln Asn Thr Ser Val 
225                 230                 235                 240 


Asp Arg Gln Leu Gln Leu Arg Gln Lys Thr Gly Leu Pro Leu Leu Ser 
                245                 250                 255     


Thr Tyr Phe Ser Cys Ser Lys Leu Arg Trp Phe Leu Asp Asn Glu Pro 
            260                 265                 270         


Leu Cys Thr Lys Ala Tyr Glu Glu Asn Asp Leu Met Phe Gly Thr Val 
        275                 280                 285             


Asp Thr Trp Leu Ile Tyr Gln Leu Thr Lys Gln Lys Ala Phe Val Ser 
    290                 295                 300                 


Asp Val Thr Asn Ala Ser Arg Thr Gly Phe Met Asn Leu Ser Thr Leu 
305                 310                 315                 320 


Lys Tyr Asp Asn Glu Leu Leu Glu Phe Trp Gly Ile Asp Lys Asn Leu 
                325                 330                 335     


Ile His Met Pro Glu Ile Val Ser Ser Ser Gln Tyr Tyr Gly Asp Phe 
            340                 345                 350         


Gly Ile Pro Asp Trp Ile Met Glu Lys Leu His Asp Ser Pro Lys Thr 
        355                 360                 365             


Val Leu Arg Asp Leu Val Lys Arg Asn Leu Pro Ile Gln Gly Cys Leu 
    370                 375                 380                 


Gly Asp Gln Ser Ala Ser Met Val Gly Gln Leu Ala Tyr Lys Pro Gly 
385                 390                 395                 400 


Ala Ala Lys Cys Thr Tyr Gly Thr Gly Cys Phe Leu Leu Tyr Asn Thr 
                405                 410                 415     


Gly Thr Lys Lys Leu Ile Ser Gln His Gly Ala Leu Thr Thr Leu Ala 
            420                 425                 430         


Phe Trp Phe Pro His Leu Gln Glu Tyr Gly Gly Gln Lys Pro Glu Leu 
        435                 440                 445             


Ser Lys Pro His Phe Ala Leu Glu Gly Ser Val Ala Val Ala Gly Ala 
    450                 455                 460                 


Val Val Gln Trp Leu Arg Asp Asn Leu Arg Leu Ile Asp Lys Ser Glu 
465                 470                 475                 480 


Asp Val Gly Pro Ile Ala Ser Thr Val Pro Asp Ser Gly Gly Val Val 
                485                 490                 495     


Phe Val Pro Ala Phe Ser Gly Leu Phe Ala Pro Tyr Trp Asp Pro Asp 
            500                 505                 510         


Ala Arg Ala Thr Ile Met Gly Met Ser Gln Phe Thr Thr Ala Ser His 
        515                 520                 525             


Ile Ala Arg Ala Ala Val Glu Gly Val Cys Phe Gln Ala Arg Ala Ile 
    530                 535                 540                 


Leu Lys Ala Met Ser Ser Asp Ala Phe Gly Glu Gly Ser Lys Asp Arg 
545                 550                 555                 560 


Asp Phe Leu Glu Glu Ile Ser Asp Val Thr Tyr Gln Lys Ser Pro Leu 
                565                 570                 575     


Ser Val Leu Ala Val Asp Gly Gly Met Ser Arg Ser Asn Glu Val Met 
            580                 585                 590         


Gln Ile Gln Ala Asp Ile Leu Gly Pro Cys Val Lys Val Arg Arg Ser 
        595                 600                 605             


Pro Thr Ala Glu Cys Thr Ala Leu Gly Ala Ala Ile Ala Ala Asn Met 
    610                 615                 620                 


Ala Phe Lys Asp Val Asn Glu Arg Pro Leu Trp Lys Asp Leu His Asp 
625                 630                 635                 640 


Val Lys Lys Trp Val Phe Tyr Asn Gly Met Glu Lys Asn Glu Gln Ile 
                645                 650                 655     


Ser Pro Glu Ala His Pro Asn Leu Lys Ile Phe Arg Ser Glu Ser Asp 
            660                 665                 670         


Asp Ala Glu Arg Arg Lys His Trp Lys Tyr Trp Glu Val Ala Val Glu 
        675                 680                 685             


Arg Ser Lys Gly Trp Leu Lys Asp Ile Glu Gly Glu His Glu Gln Val 
    690                 695                 700                 


Leu Glu Asn Phe Gln 
705                 


<210>  5
<211>  3045
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(3045)
<223>  KGD1 wild-type gene encoding wild-type Kgd1p protein

<400>  5
atg cta agg ttc gtg tct tcg caa acc tgc cgg tat agt tca aga gga         48
Met Leu Arg Phe Val Ser Ser Gln Thr Cys Arg Tyr Ser Ser Arg Gly           
1               5                   10                  15                

cta tta aaa aca tct tta ctt aaa aat gca tct act gtc aaa att gtc         96
Leu Leu Lys Thr Ser Leu Leu Lys Asn Ala Ser Thr Val Lys Ile Val           
            20                  25                  30                    

gga aga ggg tta gcc acc gct ggt aca gat aat ttt cta tcg aca tca        144
Gly Arg Gly Leu Ala Thr Ala Gly Thr Asp Asn Phe Leu Ser Thr Ser           
        35                  40                  45                        

aat gcc acc tat atc gat gaa atg tac caa gct tgg caa aaa gac cca        192
Asn Ala Thr Tyr Ile Asp Glu Met Tyr Gln Ala Trp Gln Lys Asp Pro           
    50                  55                  60                            

tct tca gtc cat gtt tca tgg gac gca tat ttc aag aat atg tct aac        240
Ser Ser Val His Val Ser Trp Asp Ala Tyr Phe Lys Asn Met Ser Asn           
65                  70                  75                  80            

cca aag att cct gct aca aag gct ttt cag gct cct ccc agt atc agt        288
Pro Lys Ile Pro Ala Thr Lys Ala Phe Gln Ala Pro Pro Ser Ile Ser           
                85                  90                  95                

aac ttt ccc cag ggt acc gaa gca gct ccc tta ggg acc gca atg act        336
Asn Phe Pro Gln Gly Thr Glu Ala Ala Pro Leu Gly Thr Ala Met Thr           
            100                 105                 110                   

ggt tca gta gat gag aac gtc tcc att cat cta aaa gtg caa ttg cta        384
Gly Ser Val Asp Glu Asn Val Ser Ile His Leu Lys Val Gln Leu Leu           
        115                 120                 125                       

tgt aga gct tac caa gtt aga ggt cat tta aaa gcc cat ata gat cct        432
Cys Arg Ala Tyr Gln Val Arg Gly His Leu Lys Ala His Ile Asp Pro           
    130                 135                 140                           

tta ggg atc tca ttt ggt agt aat aaa aat aac cct gtt cct ccg gaa        480
Leu Gly Ile Ser Phe Gly Ser Asn Lys Asn Asn Pro Val Pro Pro Glu           
145                 150                 155                 160           

ttg act cta gac tac tac ggc ttt agc aaa cac gat ctt gat aaa gaa        528
Leu Thr Leu Asp Tyr Tyr Gly Phe Ser Lys His Asp Leu Asp Lys Glu           
                165                 170                 175               

atc aac cta gga cct ggt atc ctg cca agg ttt gca agg gac ggg aaa        576
Ile Asn Leu Gly Pro Gly Ile Leu Pro Arg Phe Ala Arg Asp Gly Lys           
            180                 185                 190                   

tct aaa atg tct ctg aaa gag att gtg gat cat cta gaa aag tta tat        624
Ser Lys Met Ser Leu Lys Glu Ile Val Asp His Leu Glu Lys Leu Tyr           
        195                 200                 205                       

tgt tcc tct tat ggg gta caa tac aca cat att cca tct aag caa aag        672
Cys Ser Ser Tyr Gly Val Gln Tyr Thr His Ile Pro Ser Lys Gln Lys           
    210                 215                 220                           

tgt gat tgg tta aga gag aga att gag att cct gaa cct tac caa tat        720
Cys Asp Trp Leu Arg Glu Arg Ile Glu Ile Pro Glu Pro Tyr Gln Tyr           
225                 230                 235                 240           

aca gtg gac caa aag aga caa atc tta gat aga tta aca tgg gcc act        768
Thr Val Asp Gln Lys Arg Gln Ile Leu Asp Arg Leu Thr Trp Ala Thr           
                245                 250                 255               

tct ttt gag tca ttc tta tct aca aaa ttt cca aat gat aag agg ttc        816
Ser Phe Glu Ser Phe Leu Ser Thr Lys Phe Pro Asn Asp Lys Arg Phe           
            260                 265                 270                   

ggt tta gaa ggt ttg gaa agt gtt gtt cca ggt att aaa act ttg gtt        864
Gly Leu Glu Gly Leu Glu Ser Val Val Pro Gly Ile Lys Thr Leu Val           
        275                 280                 285                       

gat cgt tct gtt gaa ttg ggt gta gaa gat att gtt ttg ggt atg gct        912
Asp Arg Ser Val Glu Leu Gly Val Glu Asp Ile Val Leu Gly Met Ala           
    290                 295                 300                           

cac cgt ggt aga ttg aac gtt tta tcc aat gtg gtc cgt aaa cca aat        960
His Arg Gly Arg Leu Asn Val Leu Ser Asn Val Val Arg Lys Pro Asn           
305                 310                 315                 320           

gaa tct att ttt tct gaa ttt aag ggt tcg agc gct cgc gat gat att       1008
Glu Ser Ile Phe Ser Glu Phe Lys Gly Ser Ser Ala Arg Asp Asp Ile           
                325                 330                 335               

gaa gga tcg ggt gat gtc aag tac cat ttg ggt atg aac tac caa aga       1056
Glu Gly Ser Gly Asp Val Lys Tyr His Leu Gly Met Asn Tyr Gln Arg           
            340                 345                 350                   

cca act acg tct ggt aag tac gtc aat tta tcg ctg gtg gca aat cct       1104
Pro Thr Thr Ser Gly Lys Tyr Val Asn Leu Ser Leu Val Ala Asn Pro           
        355                 360                 365                       

tct cat tta gaa tcc caa gat cca gtt gtt ctt ggt aga act aga gct       1152
Ser His Leu Glu Ser Gln Asp Pro Val Val Leu Gly Arg Thr Arg Ala           
    370                 375                 380                           

tta ttg cat gcc aag aac gat ttg aag gaa aaa aca aag gcc tta ggt       1200
Leu Leu His Ala Lys Asn Asp Leu Lys Glu Lys Thr Lys Ala Leu Gly           
385                 390                 395                 400           

gtg tta tta cat ggt gat gct gct ttt gct ggg cag ggt gtt gtt tat       1248
Val Leu Leu His Gly Asp Ala Ala Phe Ala Gly Gln Gly Val Val Tyr           
                405                 410                 415               

gaa acc atg ggt ttc ttg acc cta cca gaa tac tct act ggt ggt act       1296
Glu Thr Met Gly Phe Leu Thr Leu Pro Glu Tyr Ser Thr Gly Gly Thr           
            420                 425                 430                   

att cat gtt att aca aac aac cag atc gga ttc act acg gat cca aga       1344
Ile His Val Ile Thr Asn Asn Gln Ile Gly Phe Thr Thr Asp Pro Arg           
        435                 440                 445                       

ttt gca agg tcc aca cca tat cct tcc gat ttg gct aag gcc att gat       1392
Phe Ala Arg Ser Thr Pro Tyr Pro Ser Asp Leu Ala Lys Ala Ile Asp           
    450                 455                 460                           

gcc cca att ttc cat gtt aac gct aat gac gtg gaa gct gtg acc ttt       1440
Ala Pro Ile Phe His Val Asn Ala Asn Asp Val Glu Ala Val Thr Phe           
465                 470                 475                 480           

att ttc aat tta gcc gca gaa tgg aga cat aag ttc cac aca gat gcc       1488
Ile Phe Asn Leu Ala Ala Glu Trp Arg His Lys Phe His Thr Asp Ala           
                485                 490                 495               

ata att gat gtc gtt ggt tgg aga aaa cat gga cat aat gaa acc gat       1536
Ile Ile Asp Val Val Gly Trp Arg Lys His Gly His Asn Glu Thr Asp           
            500                 505                 510                   

cag cca tcg ttt act caa cca tta atg tac aaa aaa att gca aaa caa       1584
Gln Pro Ser Phe Thr Gln Pro Leu Met Tyr Lys Lys Ile Ala Lys Gln           
        515                 520                 525                       

aaa tct gtc att gac gtc tat acg gaa aaa ttg ata agt gaa ggc aca       1632
Lys Ser Val Ile Asp Val Tyr Thr Glu Lys Leu Ile Ser Glu Gly Thr           
    530                 535                 540                           

ttt tct aaa aaa gat att gat gag cac aag aaa tgg gta tgg aac tta       1680
Phe Ser Lys Lys Asp Ile Asp Glu His Lys Lys Trp Val Trp Asn Leu           
545                 550                 555                 560           

ttt gaa gat gct ttc gaa aag gca aag gat tac gtc cca tct caa aga       1728
Phe Glu Asp Ala Phe Glu Lys Ala Lys Asp Tyr Val Pro Ser Gln Arg           
                565                 570                 575               

gaa tgg tta act gct gcc tgg gaa gga ttc aaa tcc cca aag gaa ttg       1776
Glu Trp Leu Thr Ala Ala Trp Glu Gly Phe Lys Ser Pro Lys Glu Leu           
            580                 585                 590                   

gcc act gag ata tta cca cat gaa cca act aat gtt cca gag agt act       1824
Ala Thr Glu Ile Leu Pro His Glu Pro Thr Asn Val Pro Glu Ser Thr           
        595                 600                 605                       

ttg aaa gaa cta ggt aag gta ctc tct tcg tgg cca gaa ggt ttt gaa       1872
Leu Lys Glu Leu Gly Lys Val Leu Ser Ser Trp Pro Glu Gly Phe Glu           
    610                 615                 620                           

gtg cac aaa aat cta aag aga att ttg aaa aat aga gga aaa tct att       1920
Val His Lys Asn Leu Lys Arg Ile Leu Lys Asn Arg Gly Lys Ser Ile           
625                 630                 635                 640           

gag aca ggt gaa ggc atc gat tgg gcc acc ggt gaa gca tta gcg ttc       1968
Glu Thr Gly Glu Gly Ile Asp Trp Ala Thr Gly Glu Ala Leu Ala Phe           
                645                 650                 655               

ggt aca ttg gtt ttg gat ggt cag aac gtt agg gtt tcc ggt gaa gat       2016
Gly Thr Leu Val Leu Asp Gly Gln Asn Val Arg Val Ser Gly Glu Asp           
            660                 665                 670                   

gta gaa aga ggt aca ttt tct caa cgt cat gca gtc ttg cat gac caa       2064
Val Glu Arg Gly Thr Phe Ser Gln Arg His Ala Val Leu His Asp Gln           
        675                 680                 685                       

caa tct gaa gcc att tac aca ccg cta agc act ctg aat aat gaa aag       2112
Gln Ser Glu Ala Ile Tyr Thr Pro Leu Ser Thr Leu Asn Asn Glu Lys           
    690                 695                 700                           

gca gac ttc acc att gca aat tcc tcg ttg tct gag tac ggt gta atg       2160
Ala Asp Phe Thr Ile Ala Asn Ser Ser Leu Ser Glu Tyr Gly Val Met           
705                 710                 715                 720           

ggt ttc gaa tat ggt tat tcg cta acc tcc cca gat tat cta gtc atg       2208
Gly Phe Glu Tyr Gly Tyr Ser Leu Thr Ser Pro Asp Tyr Leu Val Met           
                725                 730                 735               

tgg gag gct caa ttc ggt gac ttt gca aat aca gca cag gtt att att       2256
Trp Glu Ala Gln Phe Gly Asp Phe Ala Asn Thr Ala Gln Val Ile Ile           
            740                 745                 750                   

gac caa ttt att gcc ggt ggt gaa caa aaa tgg aag caa cgc tct ggt       2304
Asp Gln Phe Ile Ala Gly Gly Glu Gln Lys Trp Lys Gln Arg Ser Gly           
        755                 760                 765                       

tta gtt ttg tct tta ccc cat ggt tat gat ggc cag ggg cca gaa cat       2352
Leu Val Leu Ser Leu Pro His Gly Tyr Asp Gly Gln Gly Pro Glu His           
    770                 775                 780                           

tcg tct ggt aga ttg gaa aga ttc ttg caa cta gcc aat gaa gac cca       2400
Ser Ser Gly Arg Leu Glu Arg Phe Leu Gln Leu Ala Asn Glu Asp Pro           
785                 790                 795                 800           

aga tat ttc cca tct gaa gaa aag cta cag aga caa cat cag gat tgt       2448
Arg Tyr Phe Pro Ser Glu Glu Lys Leu Gln Arg Gln His Gln Asp Cys           
                805                 810                 815               

aat ttc cag gtt gtt tat cca act acg cct gct aat tta ttc cac att       2496
Asn Phe Gln Val Val Tyr Pro Thr Thr Pro Ala Asn Leu Phe His Ile           
            820                 825                 830                   

cta agg aga cag caa cat cgt caa ttc cgt aaa cca ttg gcg tta ttc       2544
Leu Arg Arg Gln Gln His Arg Gln Phe Arg Lys Pro Leu Ala Leu Phe           
        835                 840                 845                       

ttt tct aaa cag ctg ctg cgt cac cca ttg gcc aga tca tct ctt tcc       2592
Phe Ser Lys Gln Leu Leu Arg His Pro Leu Ala Arg Ser Ser Leu Ser           
    850                 855                 860                           

gaa ttc acg gaa ggc gga ttc caa tgg att atc gaa gat att gaa cat       2640
Glu Phe Thr Glu Gly Gly Phe Gln Trp Ile Ile Glu Asp Ile Glu His           
865                 870                 875                 880           

gga aaa agt att ggt acg aaa gag gaa acc aag aga tta gtt ttg ctg       2688
Gly Lys Ser Ile Gly Thr Lys Glu Glu Thr Lys Arg Leu Val Leu Leu           
                885                 890                 895               

agt ggc caa gtg tac act gcc cta cat aaa aga cgt gaa agt ttg ggt       2736
Ser Gly Gln Val Tyr Thr Ala Leu His Lys Arg Arg Glu Ser Leu Gly           
            900                 905                 910                   

gat aag acc act gct ttc tta aag att gaa gag ctg cac cca ttc cca       2784
Asp Lys Thr Thr Ala Phe Leu Lys Ile Glu Glu Leu His Pro Phe Pro           
        915                 920                 925                       

ttt gct cag cta cgt gat tca tta aat tct tat cca aac ttg gaa gaa       2832
Phe Ala Gln Leu Arg Asp Ser Leu Asn Ser Tyr Pro Asn Leu Glu Glu           
    930                 935                 940                           

att gtt tgg tgc cag gaa gag cca ttg aac atg ggt tcg tgg gca tac       2880
Ile Val Trp Cys Gln Glu Glu Pro Leu Asn Met Gly Ser Trp Ala Tyr           
945                 950                 955                 960           

aca gaa cca cgc tta cac aca aca tta aaa gaa acg gat aaa tat aag       2928
Thr Glu Pro Arg Leu His Thr Thr Leu Lys Glu Thr Asp Lys Tyr Lys           
                965                 970                 975               

gat ttc aag gtc aga tac tgt ggt aga aac cca agt ggt gct gtt gct       2976
Asp Phe Lys Val Arg Tyr Cys Gly Arg Asn Pro Ser Gly Ala Val Ala           
            980                 985                 990                   

gcc ggt agc aaa tca cta cat ttg  gcc gaa gaa gat gcc  ttt ttg aaa     3024
Ala Gly Ser Lys Ser Leu His Leu  Ala Glu Glu Asp Ala  Phe Leu Lys         
        995                 1000                 1005                     

gat gtt  ttc caa caa tcc taa                                          3045
Asp Val  Phe Gln Gln Ser                                                  
    1010                                                                  


<210>  6
<211>  1014
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  6

Met Leu Arg Phe Val Ser Ser Gln Thr Cys Arg Tyr Ser Ser Arg Gly 
1               5                   10                  15      


Leu Leu Lys Thr Ser Leu Leu Lys Asn Ala Ser Thr Val Lys Ile Val 
            20                  25                  30          


Gly Arg Gly Leu Ala Thr Ala Gly Thr Asp Asn Phe Leu Ser Thr Ser 
        35                  40                  45              


Asn Ala Thr Tyr Ile Asp Glu Met Tyr Gln Ala Trp Gln Lys Asp Pro 
    50                  55                  60                  


Ser Ser Val His Val Ser Trp Asp Ala Tyr Phe Lys Asn Met Ser Asn 
65                  70                  75                  80  


Pro Lys Ile Pro Ala Thr Lys Ala Phe Gln Ala Pro Pro Ser Ile Ser 
                85                  90                  95      


Asn Phe Pro Gln Gly Thr Glu Ala Ala Pro Leu Gly Thr Ala Met Thr 
            100                 105                 110         


Gly Ser Val Asp Glu Asn Val Ser Ile His Leu Lys Val Gln Leu Leu 
        115                 120                 125             


Cys Arg Ala Tyr Gln Val Arg Gly His Leu Lys Ala His Ile Asp Pro 
    130                 135                 140                 


Leu Gly Ile Ser Phe Gly Ser Asn Lys Asn Asn Pro Val Pro Pro Glu 
145                 150                 155                 160 


Leu Thr Leu Asp Tyr Tyr Gly Phe Ser Lys His Asp Leu Asp Lys Glu 
                165                 170                 175     


Ile Asn Leu Gly Pro Gly Ile Leu Pro Arg Phe Ala Arg Asp Gly Lys 
            180                 185                 190         


Ser Lys Met Ser Leu Lys Glu Ile Val Asp His Leu Glu Lys Leu Tyr 
        195                 200                 205             


Cys Ser Ser Tyr Gly Val Gln Tyr Thr His Ile Pro Ser Lys Gln Lys 
    210                 215                 220                 


Cys Asp Trp Leu Arg Glu Arg Ile Glu Ile Pro Glu Pro Tyr Gln Tyr 
225                 230                 235                 240 


Thr Val Asp Gln Lys Arg Gln Ile Leu Asp Arg Leu Thr Trp Ala Thr 
                245                 250                 255     


Ser Phe Glu Ser Phe Leu Ser Thr Lys Phe Pro Asn Asp Lys Arg Phe 
            260                 265                 270         


Gly Leu Glu Gly Leu Glu Ser Val Val Pro Gly Ile Lys Thr Leu Val 
        275                 280                 285             


Asp Arg Ser Val Glu Leu Gly Val Glu Asp Ile Val Leu Gly Met Ala 
    290                 295                 300                 


His Arg Gly Arg Leu Asn Val Leu Ser Asn Val Val Arg Lys Pro Asn 
305                 310                 315                 320 


Glu Ser Ile Phe Ser Glu Phe Lys Gly Ser Ser Ala Arg Asp Asp Ile 
                325                 330                 335     


Glu Gly Ser Gly Asp Val Lys Tyr His Leu Gly Met Asn Tyr Gln Arg 
            340                 345                 350         


Pro Thr Thr Ser Gly Lys Tyr Val Asn Leu Ser Leu Val Ala Asn Pro 
        355                 360                 365             


Ser His Leu Glu Ser Gln Asp Pro Val Val Leu Gly Arg Thr Arg Ala 
    370                 375                 380                 


Leu Leu His Ala Lys Asn Asp Leu Lys Glu Lys Thr Lys Ala Leu Gly 
385                 390                 395                 400 


Val Leu Leu His Gly Asp Ala Ala Phe Ala Gly Gln Gly Val Val Tyr 
                405                 410                 415     


Glu Thr Met Gly Phe Leu Thr Leu Pro Glu Tyr Ser Thr Gly Gly Thr 
            420                 425                 430         


Ile His Val Ile Thr Asn Asn Gln Ile Gly Phe Thr Thr Asp Pro Arg 
        435                 440                 445             


Phe Ala Arg Ser Thr Pro Tyr Pro Ser Asp Leu Ala Lys Ala Ile Asp 
    450                 455                 460                 


Ala Pro Ile Phe His Val Asn Ala Asn Asp Val Glu Ala Val Thr Phe 
465                 470                 475                 480 


Ile Phe Asn Leu Ala Ala Glu Trp Arg His Lys Phe His Thr Asp Ala 
                485                 490                 495     


Ile Ile Asp Val Val Gly Trp Arg Lys His Gly His Asn Glu Thr Asp 
            500                 505                 510         


Gln Pro Ser Phe Thr Gln Pro Leu Met Tyr Lys Lys Ile Ala Lys Gln 
        515                 520                 525             


Lys Ser Val Ile Asp Val Tyr Thr Glu Lys Leu Ile Ser Glu Gly Thr 
    530                 535                 540                 


Phe Ser Lys Lys Asp Ile Asp Glu His Lys Lys Trp Val Trp Asn Leu 
545                 550                 555                 560 


Phe Glu Asp Ala Phe Glu Lys Ala Lys Asp Tyr Val Pro Ser Gln Arg 
                565                 570                 575     


Glu Trp Leu Thr Ala Ala Trp Glu Gly Phe Lys Ser Pro Lys Glu Leu 
            580                 585                 590         


Ala Thr Glu Ile Leu Pro His Glu Pro Thr Asn Val Pro Glu Ser Thr 
        595                 600                 605             


Leu Lys Glu Leu Gly Lys Val Leu Ser Ser Trp Pro Glu Gly Phe Glu 
    610                 615                 620                 


Val His Lys Asn Leu Lys Arg Ile Leu Lys Asn Arg Gly Lys Ser Ile 
625                 630                 635                 640 


Glu Thr Gly Glu Gly Ile Asp Trp Ala Thr Gly Glu Ala Leu Ala Phe 
                645                 650                 655     


Gly Thr Leu Val Leu Asp Gly Gln Asn Val Arg Val Ser Gly Glu Asp 
            660                 665                 670         


Val Glu Arg Gly Thr Phe Ser Gln Arg His Ala Val Leu His Asp Gln 
        675                 680                 685             


Gln Ser Glu Ala Ile Tyr Thr Pro Leu Ser Thr Leu Asn Asn Glu Lys 
    690                 695                 700                 


Ala Asp Phe Thr Ile Ala Asn Ser Ser Leu Ser Glu Tyr Gly Val Met 
705                 710                 715                 720 


Gly Phe Glu Tyr Gly Tyr Ser Leu Thr Ser Pro Asp Tyr Leu Val Met 
                725                 730                 735     


Trp Glu Ala Gln Phe Gly Asp Phe Ala Asn Thr Ala Gln Val Ile Ile 
            740                 745                 750         


Asp Gln Phe Ile Ala Gly Gly Glu Gln Lys Trp Lys Gln Arg Ser Gly 
        755                 760                 765             


Leu Val Leu Ser Leu Pro His Gly Tyr Asp Gly Gln Gly Pro Glu His 
    770                 775                 780                 


Ser Ser Gly Arg Leu Glu Arg Phe Leu Gln Leu Ala Asn Glu Asp Pro 
785                 790                 795                 800 


Arg Tyr Phe Pro Ser Glu Glu Lys Leu Gln Arg Gln His Gln Asp Cys 
                805                 810                 815     


Asn Phe Gln Val Val Tyr Pro Thr Thr Pro Ala Asn Leu Phe His Ile 
            820                 825                 830         


Leu Arg Arg Gln Gln His Arg Gln Phe Arg Lys Pro Leu Ala Leu Phe 
        835                 840                 845             


Phe Ser Lys Gln Leu Leu Arg His Pro Leu Ala Arg Ser Ser Leu Ser 
    850                 855                 860                 


Glu Phe Thr Glu Gly Gly Phe Gln Trp Ile Ile Glu Asp Ile Glu His 
865                 870                 875                 880 


Gly Lys Ser Ile Gly Thr Lys Glu Glu Thr Lys Arg Leu Val Leu Leu 
                885                 890                 895     


Ser Gly Gln Val Tyr Thr Ala Leu His Lys Arg Arg Glu Ser Leu Gly 
            900                 905                 910         


Asp Lys Thr Thr Ala Phe Leu Lys Ile Glu Glu Leu His Pro Phe Pro 
        915                 920                 925             


Phe Ala Gln Leu Arg Asp Ser Leu Asn Ser Tyr Pro Asn Leu Glu Glu 
    930                 935                 940                 


Ile Val Trp Cys Gln Glu Glu Pro Leu Asn Met Gly Ser Trp Ala Tyr 
945                 950                 955                 960 


Thr Glu Pro Arg Leu His Thr Thr Leu Lys Glu Thr Asp Lys Tyr Lys 
                965                 970                 975     


Asp Phe Lys Val Arg Tyr Cys Gly Arg Asn Pro Ser Gly Ala Val Ala 
            980                 985                 990         


Ala Gly Ser Lys Ser Leu His Leu  Ala Glu Glu Asp Ala  Phe Leu Lys 
        995                 1000                 1005             


Asp Val  Phe Gln Gln Ser 
    1010                 


<210>  7
<211>  3045
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(3045)
<223>  KGD1_1 mutant gene (C2969A) encoding mutant Kgd1_1 protein 
       (A990D)

<400>  7
atg cta agg ttc gtg tct tcg caa acc tgc cgg tat agt tca aga gga         48
Met Leu Arg Phe Val Ser Ser Gln Thr Cys Arg Tyr Ser Ser Arg Gly           
1               5                   10                  15                

cta tta aaa aca tct tta ctt aaa aat gca tct act gtc aaa att gtc         96
Leu Leu Lys Thr Ser Leu Leu Lys Asn Ala Ser Thr Val Lys Ile Val           
            20                  25                  30                    

gga aga ggg tta gcc acc gct ggt aca gat aat ttt cta tcg aca tca        144
Gly Arg Gly Leu Ala Thr Ala Gly Thr Asp Asn Phe Leu Ser Thr Ser           
        35                  40                  45                        

aat gcc acc tat atc gat gaa atg tac caa gct tgg caa aaa gac cca        192
Asn Ala Thr Tyr Ile Asp Glu Met Tyr Gln Ala Trp Gln Lys Asp Pro           
    50                  55                  60                            

tct tca gtc cat gtt tca tgg gac gca tat ttc aag aat atg tct aac        240
Ser Ser Val His Val Ser Trp Asp Ala Tyr Phe Lys Asn Met Ser Asn           
65                  70                  75                  80            

cca aag att cct gct aca aag gct ttt cag gct cct ccc agt atc agt        288
Pro Lys Ile Pro Ala Thr Lys Ala Phe Gln Ala Pro Pro Ser Ile Ser           
                85                  90                  95                

aac ttt ccc cag ggt acc gaa gca gct ccc tta ggg acc gca atg act        336
Asn Phe Pro Gln Gly Thr Glu Ala Ala Pro Leu Gly Thr Ala Met Thr           
            100                 105                 110                   

ggt tca gta gat gag aac gtc tcc att cat cta aaa gtg caa ttg cta        384
Gly Ser Val Asp Glu Asn Val Ser Ile His Leu Lys Val Gln Leu Leu           
        115                 120                 125                       

tgt aga gct tac caa gtt aga ggt cat tta aaa gcc cat ata gat cct        432
Cys Arg Ala Tyr Gln Val Arg Gly His Leu Lys Ala His Ile Asp Pro           
    130                 135                 140                           

tta ggg atc tca ttt ggt agt aat aaa aat aac cct gtt cct ccg gaa        480
Leu Gly Ile Ser Phe Gly Ser Asn Lys Asn Asn Pro Val Pro Pro Glu           
145                 150                 155                 160           

ttg act cta gac tac tac ggc ttt agc aaa cac gat ctt gat aaa gaa        528
Leu Thr Leu Asp Tyr Tyr Gly Phe Ser Lys His Asp Leu Asp Lys Glu           
                165                 170                 175               

atc aac cta gga cct ggt atc ctg cca agg ttt gca agg gac ggg aaa        576
Ile Asn Leu Gly Pro Gly Ile Leu Pro Arg Phe Ala Arg Asp Gly Lys           
            180                 185                 190                   

tct aaa atg tct ctg aaa gag att gtg gat cat cta gaa aag tta tat        624
Ser Lys Met Ser Leu Lys Glu Ile Val Asp His Leu Glu Lys Leu Tyr           
        195                 200                 205                       

tgt tcc tct tat ggg gta caa tac aca cat att cca tct aag caa aag        672
Cys Ser Ser Tyr Gly Val Gln Tyr Thr His Ile Pro Ser Lys Gln Lys           
    210                 215                 220                           

tgt gat tgg tta aga gag aga att gag att cct gaa cct tac caa tat        720
Cys Asp Trp Leu Arg Glu Arg Ile Glu Ile Pro Glu Pro Tyr Gln Tyr           
225                 230                 235                 240           

aca gtg gac caa aag aga caa atc tta gat aga tta aca tgg gcc act        768
Thr Val Asp Gln Lys Arg Gln Ile Leu Asp Arg Leu Thr Trp Ala Thr           
                245                 250                 255               

tct ttt gag tca ttc tta tct aca aaa ttt cca aat gat aag agg ttc        816
Ser Phe Glu Ser Phe Leu Ser Thr Lys Phe Pro Asn Asp Lys Arg Phe           
            260                 265                 270                   

ggt tta gaa ggt ttg gaa agt gtt gtt cca ggt att aaa act ttg gtt        864
Gly Leu Glu Gly Leu Glu Ser Val Val Pro Gly Ile Lys Thr Leu Val           
        275                 280                 285                       

gat cgt tct gtt gaa ttg ggt gta gaa gat att gtt ttg ggt atg gct        912
Asp Arg Ser Val Glu Leu Gly Val Glu Asp Ile Val Leu Gly Met Ala           
    290                 295                 300                           

cac cgt ggt aga ttg aac gtt tta tcc aat gtg gtc cgt aaa cca aat        960
His Arg Gly Arg Leu Asn Val Leu Ser Asn Val Val Arg Lys Pro Asn           
305                 310                 315                 320           

gaa tct att ttt tct gaa ttt aag ggt tcg agc gct cgc gat gat att       1008
Glu Ser Ile Phe Ser Glu Phe Lys Gly Ser Ser Ala Arg Asp Asp Ile           
                325                 330                 335               

gaa gga tcg ggt gat gtc aag tac cat ttg ggt atg aac tac caa aga       1056
Glu Gly Ser Gly Asp Val Lys Tyr His Leu Gly Met Asn Tyr Gln Arg           
            340                 345                 350                   

cca act acg tct ggt aag tac gtc aat tta tcg ctg gtg gca aat cct       1104
Pro Thr Thr Ser Gly Lys Tyr Val Asn Leu Ser Leu Val Ala Asn Pro           
        355                 360                 365                       

tct cat tta gaa tcc caa gat cca gtt gtt ctt ggt aga act aga gct       1152
Ser His Leu Glu Ser Gln Asp Pro Val Val Leu Gly Arg Thr Arg Ala           
    370                 375                 380                           

tta ttg cat gcc aag aac gat ttg aag gaa aaa aca aag gcc tta ggt       1200
Leu Leu His Ala Lys Asn Asp Leu Lys Glu Lys Thr Lys Ala Leu Gly           
385                 390                 395                 400           

gtg tta tta cat ggt gat gct gct ttt gct ggg cag ggt gtt gtt tat       1248
Val Leu Leu His Gly Asp Ala Ala Phe Ala Gly Gln Gly Val Val Tyr           
                405                 410                 415               

gaa acc atg ggt ttc ttg acc cta cca gaa tac tct act ggt ggt act       1296
Glu Thr Met Gly Phe Leu Thr Leu Pro Glu Tyr Ser Thr Gly Gly Thr           
            420                 425                 430                   

att cat gtt att aca aac aac cag atc gga ttc act acg gat cca aga       1344
Ile His Val Ile Thr Asn Asn Gln Ile Gly Phe Thr Thr Asp Pro Arg           
        435                 440                 445                       

ttt gca agg tcc aca cca tat cct tcc gat ttg gct aag gcc att gat       1392
Phe Ala Arg Ser Thr Pro Tyr Pro Ser Asp Leu Ala Lys Ala Ile Asp           
    450                 455                 460                           

gcc cca att ttc cat gtt aac gct aat gac gtg gaa gct gtg acc ttt       1440
Ala Pro Ile Phe His Val Asn Ala Asn Asp Val Glu Ala Val Thr Phe           
465                 470                 475                 480           

att ttc aat tta gcc gca gaa tgg aga cat aag ttc cac aca gat gcc       1488
Ile Phe Asn Leu Ala Ala Glu Trp Arg His Lys Phe His Thr Asp Ala           
                485                 490                 495               

ata att gat gtc gtt ggt tgg aga aaa cat gga cat aat gaa acc gat       1536
Ile Ile Asp Val Val Gly Trp Arg Lys His Gly His Asn Glu Thr Asp           
            500                 505                 510                   

cag cca tcg ttt act caa cca tta atg tac aaa aaa att gca aaa caa       1584
Gln Pro Ser Phe Thr Gln Pro Leu Met Tyr Lys Lys Ile Ala Lys Gln           
        515                 520                 525                       

aaa tct gtc att gac gtc tat acg gaa aaa ttg ata agt gaa ggc aca       1632
Lys Ser Val Ile Asp Val Tyr Thr Glu Lys Leu Ile Ser Glu Gly Thr           
    530                 535                 540                           

ttt tct aaa aaa gat att gat gag cac aag aaa tgg gta tgg aac tta       1680
Phe Ser Lys Lys Asp Ile Asp Glu His Lys Lys Trp Val Trp Asn Leu           
545                 550                 555                 560           

ttt gaa gat gct ttc gaa aag gca aag gat tac gtc cca tct caa aga       1728
Phe Glu Asp Ala Phe Glu Lys Ala Lys Asp Tyr Val Pro Ser Gln Arg           
                565                 570                 575               

gaa tgg tta act gct gcc tgg gaa gga ttc aaa tcc cca aag gaa ttg       1776
Glu Trp Leu Thr Ala Ala Trp Glu Gly Phe Lys Ser Pro Lys Glu Leu           
            580                 585                 590                   

gcc act gag ata tta cca cat gaa cca act aat gtt cca gag agt act       1824
Ala Thr Glu Ile Leu Pro His Glu Pro Thr Asn Val Pro Glu Ser Thr           
        595                 600                 605                       

ttg aaa gaa cta ggt aag gta ctc tct tcg tgg cca gaa ggt ttt gaa       1872
Leu Lys Glu Leu Gly Lys Val Leu Ser Ser Trp Pro Glu Gly Phe Glu           
    610                 615                 620                           

gtg cac aaa aat cta aag aga att ttg aaa aat aga gga aaa tct att       1920
Val His Lys Asn Leu Lys Arg Ile Leu Lys Asn Arg Gly Lys Ser Ile           
625                 630                 635                 640           

gag aca ggt gaa ggc atc gat tgg gcc acc ggt gaa gca tta gcg ttc       1968
Glu Thr Gly Glu Gly Ile Asp Trp Ala Thr Gly Glu Ala Leu Ala Phe           
                645                 650                 655               

ggt aca ttg gtt ttg gat ggt cag aac gtt agg gtt tcc ggt gaa gat       2016
Gly Thr Leu Val Leu Asp Gly Gln Asn Val Arg Val Ser Gly Glu Asp           
            660                 665                 670                   

gta gaa aga ggt aca ttt tct caa cgt cat gca gtc ttg cat gac caa       2064
Val Glu Arg Gly Thr Phe Ser Gln Arg His Ala Val Leu His Asp Gln           
        675                 680                 685                       

caa tct gaa gcc att tac aca ccg cta agc act ctg aat aat gaa aag       2112
Gln Ser Glu Ala Ile Tyr Thr Pro Leu Ser Thr Leu Asn Asn Glu Lys           
    690                 695                 700                           

gca gac ttc acc att gca aat tcc tcg ttg tct gag tac ggt gta atg       2160
Ala Asp Phe Thr Ile Ala Asn Ser Ser Leu Ser Glu Tyr Gly Val Met           
705                 710                 715                 720           

ggt ttc gaa tat ggt tat tcg cta acc tcc cca gat tat cta gtc atg       2208
Gly Phe Glu Tyr Gly Tyr Ser Leu Thr Ser Pro Asp Tyr Leu Val Met           
                725                 730                 735               

tgg gag gct caa ttc ggt gac ttt gca aat aca gca cag gtt att att       2256
Trp Glu Ala Gln Phe Gly Asp Phe Ala Asn Thr Ala Gln Val Ile Ile           
            740                 745                 750                   

gac caa ttt att gcc ggt ggt gaa caa aaa tgg aag caa cgc tct ggt       2304
Asp Gln Phe Ile Ala Gly Gly Glu Gln Lys Trp Lys Gln Arg Ser Gly           
        755                 760                 765                       

tta gtt ttg tct tta ccc cat ggt tat gat ggc cag ggg cca gaa cat       2352
Leu Val Leu Ser Leu Pro His Gly Tyr Asp Gly Gln Gly Pro Glu His           
    770                 775                 780                           

tcg tct ggt aga ttg gaa aga ttc ttg caa cta gcc aat gaa gac cca       2400
Ser Ser Gly Arg Leu Glu Arg Phe Leu Gln Leu Ala Asn Glu Asp Pro           
785                 790                 795                 800           

aga tat ttc cca tct gaa gaa aag cta cag aga caa cat cag gat tgt       2448
Arg Tyr Phe Pro Ser Glu Glu Lys Leu Gln Arg Gln His Gln Asp Cys           
                805                 810                 815               

aat ttc cag gtt gtt tat cca act acg cct gct aat tta ttc cac att       2496
Asn Phe Gln Val Val Tyr Pro Thr Thr Pro Ala Asn Leu Phe His Ile           
            820                 825                 830                   

cta agg aga cag caa cat cgt caa ttc cgt aaa cca ttg gcg tta ttc       2544
Leu Arg Arg Gln Gln His Arg Gln Phe Arg Lys Pro Leu Ala Leu Phe           
        835                 840                 845                       

ttt tct aaa cag ctg ctg cgt cac cca ttg gcc aga tca tct ctt tcc       2592
Phe Ser Lys Gln Leu Leu Arg His Pro Leu Ala Arg Ser Ser Leu Ser           
    850                 855                 860                           

gaa ttc acg gaa ggc gga ttc caa tgg att atc gaa gat att gaa cat       2640
Glu Phe Thr Glu Gly Gly Phe Gln Trp Ile Ile Glu Asp Ile Glu His           
865                 870                 875                 880           

gga aaa agt att ggt acg aaa gag gaa acc aag aga tta gtt ttg ctg       2688
Gly Lys Ser Ile Gly Thr Lys Glu Glu Thr Lys Arg Leu Val Leu Leu           
                885                 890                 895               

agt ggc caa gtg tac act gcc cta cat aaa aga cgt gaa agt ttg ggt       2736
Ser Gly Gln Val Tyr Thr Ala Leu His Lys Arg Arg Glu Ser Leu Gly           
            900                 905                 910                   

gat aag acc act gct ttc tta aag att gaa gag ctg cac cca ttc cca       2784
Asp Lys Thr Thr Ala Phe Leu Lys Ile Glu Glu Leu His Pro Phe Pro           
        915                 920                 925                       

ttt gct cag cta cgt gat tca tta aat tct tat cca aac ttg gaa gaa       2832
Phe Ala Gln Leu Arg Asp Ser Leu Asn Ser Tyr Pro Asn Leu Glu Glu           
    930                 935                 940                           

att gtt tgg tgc cag gaa gag cca ttg aac atg ggt tcg tgg gca tac       2880
Ile Val Trp Cys Gln Glu Glu Pro Leu Asn Met Gly Ser Trp Ala Tyr           
945                 950                 955                 960           

aca gaa cca cgc tta cac aca aca tta aaa gaa acg gat aaa tat aag       2928
Thr Glu Pro Arg Leu His Thr Thr Leu Lys Glu Thr Asp Lys Tyr Lys           
                965                 970                 975               

gat ttc aag gtc aga tac tgt ggt aga aac cca agt ggt gat gtt gct       2976
Asp Phe Lys Val Arg Tyr Cys Gly Arg Asn Pro Ser Gly Asp Val Ala           
            980                 985                 990                   

gcc ggt agc aaa tca cta cat ttg  gcc gaa gaa gat gcc  ttt ttg aaa     3024
Ala Gly Ser Lys Ser Leu His Leu  Ala Glu Glu Asp Ala  Phe Leu Lys         
        995                 1000                 1005                     

gat gtt  ttc caa caa tcc taa                                          3045
Asp Val  Phe Gln Gln Ser                                                  
    1010                                                                  


<210>  8
<211>  1014
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  8

Met Leu Arg Phe Val Ser Ser Gln Thr Cys Arg Tyr Ser Ser Arg Gly 
1               5                   10                  15      


Leu Leu Lys Thr Ser Leu Leu Lys Asn Ala Ser Thr Val Lys Ile Val 
            20                  25                  30          


Gly Arg Gly Leu Ala Thr Ala Gly Thr Asp Asn Phe Leu Ser Thr Ser 
        35                  40                  45              


Asn Ala Thr Tyr Ile Asp Glu Met Tyr Gln Ala Trp Gln Lys Asp Pro 
    50                  55                  60                  


Ser Ser Val His Val Ser Trp Asp Ala Tyr Phe Lys Asn Met Ser Asn 
65                  70                  75                  80  


Pro Lys Ile Pro Ala Thr Lys Ala Phe Gln Ala Pro Pro Ser Ile Ser 
                85                  90                  95      


Asn Phe Pro Gln Gly Thr Glu Ala Ala Pro Leu Gly Thr Ala Met Thr 
            100                 105                 110         


Gly Ser Val Asp Glu Asn Val Ser Ile His Leu Lys Val Gln Leu Leu 
        115                 120                 125             


Cys Arg Ala Tyr Gln Val Arg Gly His Leu Lys Ala His Ile Asp Pro 
    130                 135                 140                 


Leu Gly Ile Ser Phe Gly Ser Asn Lys Asn Asn Pro Val Pro Pro Glu 
145                 150                 155                 160 


Leu Thr Leu Asp Tyr Tyr Gly Phe Ser Lys His Asp Leu Asp Lys Glu 
                165                 170                 175     


Ile Asn Leu Gly Pro Gly Ile Leu Pro Arg Phe Ala Arg Asp Gly Lys 
            180                 185                 190         


Ser Lys Met Ser Leu Lys Glu Ile Val Asp His Leu Glu Lys Leu Tyr 
        195                 200                 205             


Cys Ser Ser Tyr Gly Val Gln Tyr Thr His Ile Pro Ser Lys Gln Lys 
    210                 215                 220                 


Cys Asp Trp Leu Arg Glu Arg Ile Glu Ile Pro Glu Pro Tyr Gln Tyr 
225                 230                 235                 240 


Thr Val Asp Gln Lys Arg Gln Ile Leu Asp Arg Leu Thr Trp Ala Thr 
                245                 250                 255     


Ser Phe Glu Ser Phe Leu Ser Thr Lys Phe Pro Asn Asp Lys Arg Phe 
            260                 265                 270         


Gly Leu Glu Gly Leu Glu Ser Val Val Pro Gly Ile Lys Thr Leu Val 
        275                 280                 285             


Asp Arg Ser Val Glu Leu Gly Val Glu Asp Ile Val Leu Gly Met Ala 
    290                 295                 300                 


His Arg Gly Arg Leu Asn Val Leu Ser Asn Val Val Arg Lys Pro Asn 
305                 310                 315                 320 


Glu Ser Ile Phe Ser Glu Phe Lys Gly Ser Ser Ala Arg Asp Asp Ile 
                325                 330                 335     


Glu Gly Ser Gly Asp Val Lys Tyr His Leu Gly Met Asn Tyr Gln Arg 
            340                 345                 350         


Pro Thr Thr Ser Gly Lys Tyr Val Asn Leu Ser Leu Val Ala Asn Pro 
        355                 360                 365             


Ser His Leu Glu Ser Gln Asp Pro Val Val Leu Gly Arg Thr Arg Ala 
    370                 375                 380                 


Leu Leu His Ala Lys Asn Asp Leu Lys Glu Lys Thr Lys Ala Leu Gly 
385                 390                 395                 400 


Val Leu Leu His Gly Asp Ala Ala Phe Ala Gly Gln Gly Val Val Tyr 
                405                 410                 415     


Glu Thr Met Gly Phe Leu Thr Leu Pro Glu Tyr Ser Thr Gly Gly Thr 
            420                 425                 430         


Ile His Val Ile Thr Asn Asn Gln Ile Gly Phe Thr Thr Asp Pro Arg 
        435                 440                 445             


Phe Ala Arg Ser Thr Pro Tyr Pro Ser Asp Leu Ala Lys Ala Ile Asp 
    450                 455                 460                 


Ala Pro Ile Phe His Val Asn Ala Asn Asp Val Glu Ala Val Thr Phe 
465                 470                 475                 480 


Ile Phe Asn Leu Ala Ala Glu Trp Arg His Lys Phe His Thr Asp Ala 
                485                 490                 495     


Ile Ile Asp Val Val Gly Trp Arg Lys His Gly His Asn Glu Thr Asp 
            500                 505                 510         


Gln Pro Ser Phe Thr Gln Pro Leu Met Tyr Lys Lys Ile Ala Lys Gln 
        515                 520                 525             


Lys Ser Val Ile Asp Val Tyr Thr Glu Lys Leu Ile Ser Glu Gly Thr 
    530                 535                 540                 


Phe Ser Lys Lys Asp Ile Asp Glu His Lys Lys Trp Val Trp Asn Leu 
545                 550                 555                 560 


Phe Glu Asp Ala Phe Glu Lys Ala Lys Asp Tyr Val Pro Ser Gln Arg 
                565                 570                 575     


Glu Trp Leu Thr Ala Ala Trp Glu Gly Phe Lys Ser Pro Lys Glu Leu 
            580                 585                 590         


Ala Thr Glu Ile Leu Pro His Glu Pro Thr Asn Val Pro Glu Ser Thr 
        595                 600                 605             


Leu Lys Glu Leu Gly Lys Val Leu Ser Ser Trp Pro Glu Gly Phe Glu 
    610                 615                 620                 


Val His Lys Asn Leu Lys Arg Ile Leu Lys Asn Arg Gly Lys Ser Ile 
625                 630                 635                 640 


Glu Thr Gly Glu Gly Ile Asp Trp Ala Thr Gly Glu Ala Leu Ala Phe 
                645                 650                 655     


Gly Thr Leu Val Leu Asp Gly Gln Asn Val Arg Val Ser Gly Glu Asp 
            660                 665                 670         


Val Glu Arg Gly Thr Phe Ser Gln Arg His Ala Val Leu His Asp Gln 
        675                 680                 685             


Gln Ser Glu Ala Ile Tyr Thr Pro Leu Ser Thr Leu Asn Asn Glu Lys 
    690                 695                 700                 


Ala Asp Phe Thr Ile Ala Asn Ser Ser Leu Ser Glu Tyr Gly Val Met 
705                 710                 715                 720 


Gly Phe Glu Tyr Gly Tyr Ser Leu Thr Ser Pro Asp Tyr Leu Val Met 
                725                 730                 735     


Trp Glu Ala Gln Phe Gly Asp Phe Ala Asn Thr Ala Gln Val Ile Ile 
            740                 745                 750         


Asp Gln Phe Ile Ala Gly Gly Glu Gln Lys Trp Lys Gln Arg Ser Gly 
        755                 760                 765             


Leu Val Leu Ser Leu Pro His Gly Tyr Asp Gly Gln Gly Pro Glu His 
    770                 775                 780                 


Ser Ser Gly Arg Leu Glu Arg Phe Leu Gln Leu Ala Asn Glu Asp Pro 
785                 790                 795                 800 


Arg Tyr Phe Pro Ser Glu Glu Lys Leu Gln Arg Gln His Gln Asp Cys 
                805                 810                 815     


Asn Phe Gln Val Val Tyr Pro Thr Thr Pro Ala Asn Leu Phe His Ile 
            820                 825                 830         


Leu Arg Arg Gln Gln His Arg Gln Phe Arg Lys Pro Leu Ala Leu Phe 
        835                 840                 845             


Phe Ser Lys Gln Leu Leu Arg His Pro Leu Ala Arg Ser Ser Leu Ser 
    850                 855                 860                 


Glu Phe Thr Glu Gly Gly Phe Gln Trp Ile Ile Glu Asp Ile Glu His 
865                 870                 875                 880 


Gly Lys Ser Ile Gly Thr Lys Glu Glu Thr Lys Arg Leu Val Leu Leu 
                885                 890                 895     


Ser Gly Gln Val Tyr Thr Ala Leu His Lys Arg Arg Glu Ser Leu Gly 
            900                 905                 910         


Asp Lys Thr Thr Ala Phe Leu Lys Ile Glu Glu Leu His Pro Phe Pro 
        915                 920                 925             


Phe Ala Gln Leu Arg Asp Ser Leu Asn Ser Tyr Pro Asn Leu Glu Glu 
    930                 935                 940                 


Ile Val Trp Cys Gln Glu Glu Pro Leu Asn Met Gly Ser Trp Ala Tyr 
945                 950                 955                 960 


Thr Glu Pro Arg Leu His Thr Thr Leu Lys Glu Thr Asp Lys Tyr Lys 
                965                 970                 975     


Asp Phe Lys Val Arg Tyr Cys Gly Arg Asn Pro Ser Gly Asp Val Ala 
            980                 985                 990         


Ala Gly Ser Lys Ser Leu His Leu  Ala Glu Glu Asp Ala  Phe Leu Lys 
        995                 1000                 1005             


Asp Val  Phe Gln Gln Ser 
    1010                 


<210>  9
<211>  1392
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(1392)
<223>  KGD2 wild type gene encoding Kgd2 protein (alpha-ketoglutarate 
       dehydrogenase)

<400>  9
atg ctt tcc aga gcg acg cgt act gca gct gcc aaa tcc tta gta aaa         48
Met Leu Ser Arg Ala Thr Arg Thr Ala Ala Ala Lys Ser Leu Val Lys           
1               5                   10                  15                

tct aaa gtg gct aga aat gtt atg gct gct tct ttc gtc aag aga cat         96
Ser Lys Val Ala Arg Asn Val Met Ala Ala Ser Phe Val Lys Arg His           
            20                  25                  30                    

gct tct aca agt ttg ttc aaa caa gct aac aag gtc gaa tcc tta ggt        144
Ala Ser Thr Ser Leu Phe Lys Gln Ala Asn Lys Val Glu Ser Leu Gly           
        35                  40                  45                        

tca ata tat tta tcc ggc aag aaa att tca gtt gcg gcg aat ccg ttc        192
Ser Ile Tyr Leu Ser Gly Lys Lys Ile Ser Val Ala Ala Asn Pro Phe           
    50                  55                  60                            

tcc ata act agc aat cgt ttt aaa tct acc tct att gaa gtt cct ccg        240
Ser Ile Thr Ser Asn Arg Phe Lys Ser Thr Ser Ile Glu Val Pro Pro           
65                  70                  75                  80            

atg gca gag tcc ctg act gaa ggc tct tta aag gaa tat act aaa aac        288
Met Ala Glu Ser Leu Thr Glu Gly Ser Leu Lys Glu Tyr Thr Lys Asn           
                85                  90                  95                

gtt ggt gat ttt att aag gag gac gag ctg ttg gcc act att gag acc        336
Val Gly Asp Phe Ile Lys Glu Asp Glu Leu Leu Ala Thr Ile Glu Thr           
            100                 105                 110                   

gat aaa att gat att gag gtc aat tcg cca gta tca ggt act gtt acg        384
Asp Lys Ile Asp Ile Glu Val Asn Ser Pro Val Ser Gly Thr Val Thr           
        115                 120                 125                       

aag cta aat ttc aaa cca gag gac act gtc act gtt ggt gag gag tta        432
Lys Leu Asn Phe Lys Pro Glu Asp Thr Val Thr Val Gly Glu Glu Leu           
    130                 135                 140                           

gct cag gtc gag cct ggt gaa gca cct gct gag ggt tct gga gaa tct        480
Ala Gln Val Glu Pro Gly Glu Ala Pro Ala Glu Gly Ser Gly Glu Ser           
145                 150                 155                 160           

aag cca gag cct acc gaa caa gcg gag cca tcg caa ggt gtc gcc gca        528
Lys Pro Glu Pro Thr Glu Gln Ala Glu Pro Ser Gln Gly Val Ala Ala           
                165                 170                 175               

agg gaa aac tca agt gag gaa acg gct tca aag aaa gaa gct gct cca        576
Arg Glu Asn Ser Ser Glu Glu Thr Ala Ser Lys Lys Glu Ala Ala Pro           
            180                 185                 190                   

aag aaa gaa gcc gct cca aag aaa gaa gtt aca gaa cca aaa aag gct        624
Lys Lys Glu Ala Ala Pro Lys Lys Glu Val Thr Glu Pro Lys Lys Ala           
        195                 200                 205                       

gat caa cca aag aag acc gtc tct aag gcg cag gaa ccc cca gta gcc        672
Asp Gln Pro Lys Lys Thr Val Ser Lys Ala Gln Glu Pro Pro Val Ala           
    210                 215                 220                           

tct aac tct ttc aca cca ttt cca cgt aca gaa acc agg gtc aaa atg        720
Ser Asn Ser Phe Thr Pro Phe Pro Arg Thr Glu Thr Arg Val Lys Met           
225                 230                 235                 240           

aac cgt atg aga ttg agg att gcc gaa aga tta aaa gag tct caa aac        768
Asn Arg Met Arg Leu Arg Ile Ala Glu Arg Leu Lys Glu Ser Gln Asn           
                245                 250                 255               

act gct gct tcc tta acc aca ttc aac gaa gtt gac atg tca gct ttg        816
Thr Ala Ala Ser Leu Thr Thr Phe Asn Glu Val Asp Met Ser Ala Leu           
            260                 265                 270                   

atg gaa atg agg aaa ctg tat aaa gat gag att att aag aag acc ggt        864
Met Glu Met Arg Lys Leu Tyr Lys Asp Glu Ile Ile Lys Lys Thr Gly           
        275                 280                 285                       

act aaa ttc gga ttc atg ggt ctt ttc tcc aaa gca tgt acc ttg gcc        912
Thr Lys Phe Gly Phe Met Gly Leu Phe Ser Lys Ala Cys Thr Leu Ala           
    290                 295                 300                           

gcc aag gat att cca gcc gtc aat ggt gcc att gaa ggt gac cag att        960
Ala Lys Asp Ile Pro Ala Val Asn Gly Ala Ile Glu Gly Asp Gln Ile           
305                 310                 315                 320           

gtt tat cgt gat tac aca gat att tct gtt gct gtg gcc act cca aag       1008
Val Tyr Arg Asp Tyr Thr Asp Ile Ser Val Ala Val Ala Thr Pro Lys           
                325                 330                 335               

ggt ttg gtt acc ccc gtc gtt cgt aat gca gag tca ttg agt gtt tta       1056
Gly Leu Val Thr Pro Val Val Arg Asn Ala Glu Ser Leu Ser Val Leu           
            340                 345                 350                   

gat att gag aac gaa att gtt cgc ttg agt cat aaa gcg cgt gat ggc       1104
Asp Ile Glu Asn Glu Ile Val Arg Leu Ser His Lys Ala Arg Asp Gly           
        355                 360                 365                       

aaa tta acc cta gaa gat atg acg ggt ggt act ttc acc ata tct aat       1152
Lys Leu Thr Leu Glu Asp Met Thr Gly Gly Thr Phe Thr Ile Ser Asn           
    370                 375                 380                           

ggt ggt gtt ttt ggt tca tta tac ggt act cct atc atc aat tca cca       1200
Gly Gly Val Phe Gly Ser Leu Tyr Gly Thr Pro Ile Ile Asn Ser Pro           
385                 390                 395                 400           

caa aca gcc gtc cta ggc ttg cat ggt gtc aaa gag aga cct gtc act       1248
Gln Thr Ala Val Leu Gly Leu His Gly Val Lys Glu Arg Pro Val Thr           
                405                 410                 415               

gtt aat gga caa att gtc tca aga cca atg atg tac ttg gct ttg act       1296
Val Asn Gly Gln Ile Val Ser Arg Pro Met Met Tyr Leu Ala Leu Thr           
            420                 425                 430                   

tat gat cat aga ttg cta gat ggt aga gaa gct gtt acc ttc ttg aag       1344
Tyr Asp His Arg Leu Leu Asp Gly Arg Glu Ala Val Thr Phe Leu Lys           
        435                 440                 445                       

act gtt aaa gag ttg att gaa gac cct aga aaa atg ttg tta tgg tga       1392
Thr Val Lys Glu Leu Ile Glu Asp Pro Arg Lys Met Leu Leu Trp               
    450                 455                 460                           


<210>  10
<211>  463
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  10

Met Leu Ser Arg Ala Thr Arg Thr Ala Ala Ala Lys Ser Leu Val Lys 
1               5                   10                  15      


Ser Lys Val Ala Arg Asn Val Met Ala Ala Ser Phe Val Lys Arg His 
            20                  25                  30          


Ala Ser Thr Ser Leu Phe Lys Gln Ala Asn Lys Val Glu Ser Leu Gly 
        35                  40                  45              


Ser Ile Tyr Leu Ser Gly Lys Lys Ile Ser Val Ala Ala Asn Pro Phe 
    50                  55                  60                  


Ser Ile Thr Ser Asn Arg Phe Lys Ser Thr Ser Ile Glu Val Pro Pro 
65                  70                  75                  80  


Met Ala Glu Ser Leu Thr Glu Gly Ser Leu Lys Glu Tyr Thr Lys Asn 
                85                  90                  95      


Val Gly Asp Phe Ile Lys Glu Asp Glu Leu Leu Ala Thr Ile Glu Thr 
            100                 105                 110         


Asp Lys Ile Asp Ile Glu Val Asn Ser Pro Val Ser Gly Thr Val Thr 
        115                 120                 125             


Lys Leu Asn Phe Lys Pro Glu Asp Thr Val Thr Val Gly Glu Glu Leu 
    130                 135                 140                 


Ala Gln Val Glu Pro Gly Glu Ala Pro Ala Glu Gly Ser Gly Glu Ser 
145                 150                 155                 160 


Lys Pro Glu Pro Thr Glu Gln Ala Glu Pro Ser Gln Gly Val Ala Ala 
                165                 170                 175     


Arg Glu Asn Ser Ser Glu Glu Thr Ala Ser Lys Lys Glu Ala Ala Pro 
            180                 185                 190         


Lys Lys Glu Ala Ala Pro Lys Lys Glu Val Thr Glu Pro Lys Lys Ala 
        195                 200                 205             


Asp Gln Pro Lys Lys Thr Val Ser Lys Ala Gln Glu Pro Pro Val Ala 
    210                 215                 220                 


Ser Asn Ser Phe Thr Pro Phe Pro Arg Thr Glu Thr Arg Val Lys Met 
225                 230                 235                 240 


Asn Arg Met Arg Leu Arg Ile Ala Glu Arg Leu Lys Glu Ser Gln Asn 
                245                 250                 255     


Thr Ala Ala Ser Leu Thr Thr Phe Asn Glu Val Asp Met Ser Ala Leu 
            260                 265                 270         


Met Glu Met Arg Lys Leu Tyr Lys Asp Glu Ile Ile Lys Lys Thr Gly 
        275                 280                 285             


Thr Lys Phe Gly Phe Met Gly Leu Phe Ser Lys Ala Cys Thr Leu Ala 
    290                 295                 300                 


Ala Lys Asp Ile Pro Ala Val Asn Gly Ala Ile Glu Gly Asp Gln Ile 
305                 310                 315                 320 


Val Tyr Arg Asp Tyr Thr Asp Ile Ser Val Ala Val Ala Thr Pro Lys 
                325                 330                 335     


Gly Leu Val Thr Pro Val Val Arg Asn Ala Glu Ser Leu Ser Val Leu 
            340                 345                 350         


Asp Ile Glu Asn Glu Ile Val Arg Leu Ser His Lys Ala Arg Asp Gly 
        355                 360                 365             


Lys Leu Thr Leu Glu Asp Met Thr Gly Gly Thr Phe Thr Ile Ser Asn 
    370                 375                 380                 


Gly Gly Val Phe Gly Ser Leu Tyr Gly Thr Pro Ile Ile Asn Ser Pro 
385                 390                 395                 400 


Gln Thr Ala Val Leu Gly Leu His Gly Val Lys Glu Arg Pro Val Thr 
                405                 410                 415     


Val Asn Gly Gln Ile Val Ser Arg Pro Met Met Tyr Leu Ala Leu Thr 
            420                 425                 430         


Tyr Asp His Arg Leu Leu Asp Gly Arg Glu Ala Val Thr Phe Leu Lys 
        435                 440                 445             


Thr Val Lys Glu Leu Ile Glu Asp Pro Arg Lys Met Leu Leu Trp 
    450                 455                 460             


<210>  11
<211>  1500
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(1500)
<223>  LPD1 wild-type gene encoding Lpd1 protein (Dihydrolipoamide 
       dehydrogenase)

<400>  11
atg tta aga atc aga tca ctc cta aat aat aag cgt gcc ttt tcg tcc         48
Met Leu Arg Ile Arg Ser Leu Leu Asn Asn Lys Arg Ala Phe Ser Ser           
1               5                   10                  15                

aca gtc agg aca ttg acc att aac aag tca cat gat gta gtc atc atc         96
Thr Val Arg Thr Leu Thr Ile Asn Lys Ser His Asp Val Val Ile Ile           
            20                  25                  30                    

ggt ggt ggc cct gct ggt tac gtg gct gct atc aaa gct gct caa ttg        144
Gly Gly Gly Pro Ala Gly Tyr Val Ala Ala Ile Lys Ala Ala Gln Leu           
        35                  40                  45                        

gga ttt aac act gca tgt gta gaa aaa aga ggc aaa tta ggc ggt acc        192
Gly Phe Asn Thr Ala Cys Val Glu Lys Arg Gly Lys Leu Gly Gly Thr           
    50                  55                  60                            

tgt ctt aac gtt gga tgt atc ccc tcc aaa gca ctt cta aat aat tct        240
Cys Leu Asn Val Gly Cys Ile Pro Ser Lys Ala Leu Leu Asn Asn Ser           
65                  70                  75                  80            

cat tta ttc cac caa atg cat acg gaa gcg caa aag aga ggt att gac        288
His Leu Phe His Gln Met His Thr Glu Ala Gln Lys Arg Gly Ile Asp           
                85                  90                  95                

gtc aac ggt gat atc aaa att aac gta gca aac ttc caa aag gct aag        336
Val Asn Gly Asp Ile Lys Ile Asn Val Ala Asn Phe Gln Lys Ala Lys           
            100                 105                 110                   

gat gac gct gtt aag caa tta act gga ggt att gag ctt ctg ttc aag        384
Asp Asp Ala Val Lys Gln Leu Thr Gly Gly Ile Glu Leu Leu Phe Lys           
        115                 120                 125                       

aaa aat aag gtc acc tat tat aaa ggt aat ggt tca ttc gaa gac gaa        432
Lys Asn Lys Val Thr Tyr Tyr Lys Gly Asn Gly Ser Phe Glu Asp Glu           
    130                 135                 140                           

acg aag atc aga gta act ccc gtt gat ggg ttg gaa ggc act gtc aag        480
Thr Lys Ile Arg Val Thr Pro Val Asp Gly Leu Glu Gly Thr Val Lys           
145                 150                 155                 160           

gaa gac cac ata cta gat gtt aag aac atc ata gtc gcc acg ggc tct        528
Glu Asp His Ile Leu Asp Val Lys Asn Ile Ile Val Ala Thr Gly Ser           
                165                 170                 175               

gaa gtt aca ccc ttc ccc ggt att gaa ata gat gag gaa aaa att gtc        576
Glu Val Thr Pro Phe Pro Gly Ile Glu Ile Asp Glu Glu Lys Ile Val           
            180                 185                 190                   

tct tca aca ggt gct ctt tcg tta aag gaa att ccc aaa aga tta acc        624
Ser Ser Thr Gly Ala Leu Ser Leu Lys Glu Ile Pro Lys Arg Leu Thr           
        195                 200                 205                       

atc att ggt gga gga atc atc gga ttg gaa atg ggt tca gtt tac tct        672
Ile Ile Gly Gly Gly Ile Ile Gly Leu Glu Met Gly Ser Val Tyr Ser           
    210                 215                 220                           

aga tta ggc tcc aag gtt act gta gta gaa ttt caa cct caa att ggt        720
Arg Leu Gly Ser Lys Val Thr Val Val Glu Phe Gln Pro Gln Ile Gly           
225                 230                 235                 240           

gca tct atg gac ggc gag gtt gcc aaa gcc acc caa aag ttc ttg aaa        768
Ala Ser Met Asp Gly Glu Val Ala Lys Ala Thr Gln Lys Phe Leu Lys           
                245                 250                 255               

aag caa ggt ttg gac ttc aaa tta agc acc aaa gtt att tct gca aag        816
Lys Gln Gly Leu Asp Phe Lys Leu Ser Thr Lys Val Ile Ser Ala Lys           
            260                 265                 270                   

aga aac gac gac aag aac gtc gtc gaa att gtt gta gaa gat act aaa        864
Arg Asn Asp Asp Lys Asn Val Val Glu Ile Val Val Glu Asp Thr Lys           
        275                 280                 285                       

acg aat aag caa gaa aat ttg gaa gct gaa gtt ttg ctg gtt gct gtt        912
Thr Asn Lys Gln Glu Asn Leu Glu Ala Glu Val Leu Leu Val Ala Val           
    290                 295                 300                           

ggt aga aga cct tac att gct ggc tta ggg gct gaa aag att gga tta        960
Gly Arg Arg Pro Tyr Ile Ala Gly Leu Gly Ala Glu Lys Ile Gly Leu           
305                 310                 315                 320           

gaa gta gac aaa agg gga cgc cta gtc att gat gac caa ttt aat tcc       1008
Glu Val Asp Lys Arg Gly Arg Leu Val Ile Asp Asp Gln Phe Asn Ser           
                325                 330                 335               

aag ttc cca cac att aaa gtg gta gga gat gtt aca ttt ggt cca atg       1056
Lys Phe Pro His Ile Lys Val Val Gly Asp Val Thr Phe Gly Pro Met           
            340                 345                 350                   

ctg gct cac aaa gcc gaa gag gaa ggt att gca gct gtc gaa atg ttg       1104
Leu Ala His Lys Ala Glu Glu Glu Gly Ile Ala Ala Val Glu Met Leu           
        355                 360                 365                       

aaa act ggt cac ggt cat gtc aac tat aac aac att cct tcg gtc atg       1152
Lys Thr Gly His Gly His Val Asn Tyr Asn Asn Ile Pro Ser Val Met           
    370                 375                 380                           

tat tct cac cca gaa gta gca tgg gtt ggt aaa acc gaa gag caa ttg       1200
Tyr Ser His Pro Glu Val Ala Trp Val Gly Lys Thr Glu Glu Gln Leu           
385                 390                 395                 400           

aaa gaa gcc ggc att gac tat aaa att ggt aag ttc ccc ttt gcg gcc       1248
Lys Glu Ala Gly Ile Asp Tyr Lys Ile Gly Lys Phe Pro Phe Ala Ala           
                405                 410                 415               

aat tca aga gcc aag acc aac caa gac act gaa ggt ttc gtg aag att       1296
Asn Ser Arg Ala Lys Thr Asn Gln Asp Thr Glu Gly Phe Val Lys Ile           
            420                 425                 430                   

ttg atc gat tcc aag acc gag cgt att ttg ggg gct cac att atc ggt       1344
Leu Ile Asp Ser Lys Thr Glu Arg Ile Leu Gly Ala His Ile Ile Gly           
        435                 440                 445                       

cca aat gcc ggt gaa atg att gct gaa gct ggc tta gcc tta gaa tat       1392
Pro Asn Ala Gly Glu Met Ile Ala Glu Ala Gly Leu Ala Leu Glu Tyr           
    450                 455                 460                           

ggc gct tcc gca gaa gat gtt gct agg gtc tgc cat gct cat cct act       1440
Gly Ala Ser Ala Glu Asp Val Ala Arg Val Cys His Ala His Pro Thr           
465                 470                 475                 480           

ttg tcc gaa gca ttt aag gaa gct aac atg gct gcc tat gat aaa gct       1488
Leu Ser Glu Ala Phe Lys Glu Ala Asn Met Ala Ala Tyr Asp Lys Ala           
                485                 490                 495               

att cat tgt tga                                                       1500
Ile His Cys                                                               
                                                                          


<210>  12
<211>  499
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  12

Met Leu Arg Ile Arg Ser Leu Leu Asn Asn Lys Arg Ala Phe Ser Ser 
1               5                   10                  15      


Thr Val Arg Thr Leu Thr Ile Asn Lys Ser His Asp Val Val Ile Ile 
            20                  25                  30          


Gly Gly Gly Pro Ala Gly Tyr Val Ala Ala Ile Lys Ala Ala Gln Leu 
        35                  40                  45              


Gly Phe Asn Thr Ala Cys Val Glu Lys Arg Gly Lys Leu Gly Gly Thr 
    50                  55                  60                  


Cys Leu Asn Val Gly Cys Ile Pro Ser Lys Ala Leu Leu Asn Asn Ser 
65                  70                  75                  80  


His Leu Phe His Gln Met His Thr Glu Ala Gln Lys Arg Gly Ile Asp 
                85                  90                  95      


Val Asn Gly Asp Ile Lys Ile Asn Val Ala Asn Phe Gln Lys Ala Lys 
            100                 105                 110         


Asp Asp Ala Val Lys Gln Leu Thr Gly Gly Ile Glu Leu Leu Phe Lys 
        115                 120                 125             


Lys Asn Lys Val Thr Tyr Tyr Lys Gly Asn Gly Ser Phe Glu Asp Glu 
    130                 135                 140                 


Thr Lys Ile Arg Val Thr Pro Val Asp Gly Leu Glu Gly Thr Val Lys 
145                 150                 155                 160 


Glu Asp His Ile Leu Asp Val Lys Asn Ile Ile Val Ala Thr Gly Ser 
                165                 170                 175     


Glu Val Thr Pro Phe Pro Gly Ile Glu Ile Asp Glu Glu Lys Ile Val 
            180                 185                 190         


Ser Ser Thr Gly Ala Leu Ser Leu Lys Glu Ile Pro Lys Arg Leu Thr 
        195                 200                 205             


Ile Ile Gly Gly Gly Ile Ile Gly Leu Glu Met Gly Ser Val Tyr Ser 
    210                 215                 220                 


Arg Leu Gly Ser Lys Val Thr Val Val Glu Phe Gln Pro Gln Ile Gly 
225                 230                 235                 240 


Ala Ser Met Asp Gly Glu Val Ala Lys Ala Thr Gln Lys Phe Leu Lys 
                245                 250                 255     


Lys Gln Gly Leu Asp Phe Lys Leu Ser Thr Lys Val Ile Ser Ala Lys 
            260                 265                 270         


Arg Asn Asp Asp Lys Asn Val Val Glu Ile Val Val Glu Asp Thr Lys 
        275                 280                 285             


Thr Asn Lys Gln Glu Asn Leu Glu Ala Glu Val Leu Leu Val Ala Val 
    290                 295                 300                 


Gly Arg Arg Pro Tyr Ile Ala Gly Leu Gly Ala Glu Lys Ile Gly Leu 
305                 310                 315                 320 


Glu Val Asp Lys Arg Gly Arg Leu Val Ile Asp Asp Gln Phe Asn Ser 
                325                 330                 335     


Lys Phe Pro His Ile Lys Val Val Gly Asp Val Thr Phe Gly Pro Met 
            340                 345                 350         


Leu Ala His Lys Ala Glu Glu Glu Gly Ile Ala Ala Val Glu Met Leu 
        355                 360                 365             


Lys Thr Gly His Gly His Val Asn Tyr Asn Asn Ile Pro Ser Val Met 
    370                 375                 380                 


Tyr Ser His Pro Glu Val Ala Trp Val Gly Lys Thr Glu Glu Gln Leu 
385                 390                 395                 400 


Lys Glu Ala Gly Ile Asp Tyr Lys Ile Gly Lys Phe Pro Phe Ala Ala 
                405                 410                 415     


Asn Ser Arg Ala Lys Thr Asn Gln Asp Thr Glu Gly Phe Val Lys Ile 
            420                 425                 430         


Leu Ile Asp Ser Lys Thr Glu Arg Ile Leu Gly Ala His Ile Ile Gly 
        435                 440                 445             


Pro Asn Ala Gly Glu Met Ile Ala Glu Ala Gly Leu Ala Leu Glu Tyr 
    450                 455                 460                 


Gly Ala Ser Ala Glu Asp Val Ala Arg Val Cys His Ala His Pro Thr 
465                 470                 475                 480 


Leu Ser Glu Ala Phe Lys Glu Ala Asn Met Ala Ala Tyr Asp Lys Ala 
                485                 490                 495     


Ile His Cys 
            


<210>  13
<211>  462
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(462)
<223>  UBC13 wild-type gene encoding Ubc13 wild-type protein (E2 
       ubiquitin-conjugating enzyme)

<400>  13
atg gca tca tta ccc aag aga ata atc aag gaa act gag aaa tta gta         48
Met Ala Ser Leu Pro Lys Arg Ile Ile Lys Glu Thr Glu Lys Leu Val           
1               5                   10                  15                

agt gac cca gta cct ggc att aca gca gaa cca cat gac gac aac tta         96
Ser Asp Pro Val Pro Gly Ile Thr Ala Glu Pro His Asp Asp Asn Leu           
            20                  25                  30                    

cgt tac ttc caa gta aca atc gaa ggc cca gaa cag tca cca tat gaa        144
Arg Tyr Phe Gln Val Thr Ile Glu Gly Pro Glu Gln Ser Pro Tyr Glu           
        35                  40                  45                        

gac ggt att ttc gaa tta gaa tta tat ctg cct gac gac tat cca atg        192
Asp Gly Ile Phe Glu Leu Glu Leu Tyr Leu Pro Asp Asp Tyr Pro Met           
    50                  55                  60                            

gaa gca ccg aaa gta cgt ttt tta acc aag ata tat cat ccc aat att        240
Glu Ala Pro Lys Val Arg Phe Leu Thr Lys Ile Tyr His Pro Asn Ile           
65                  70                  75                  80            

gat agg ttg ggc cgt ata tgt ctt gat gtg ctt aaa act aat tgg tca        288
Asp Arg Leu Gly Arg Ile Cys Leu Asp Val Leu Lys Thr Asn Trp Ser           
                85                  90                  95                

ccc gct tta cag atc aga acc gtc ctg ctg tcc att caa gca ctg ttg        336
Pro Ala Leu Gln Ile Arg Thr Val Leu Leu Ser Ile Gln Ala Leu Leu           
            100                 105                 110                   

gct agc cct aat cct aat gat ccg cta gct aat gac gtc gct gaa gat        384
Ala Ser Pro Asn Pro Asn Asp Pro Leu Ala Asn Asp Val Ala Glu Asp           
        115                 120                 125                       

tgg ata aag aat gag caa ggt gcc aaa gct aag gct cgc gaa tgg acg        432
Trp Ile Lys Asn Glu Gln Gly Ala Lys Ala Lys Ala Arg Glu Trp Thr           
    130                 135                 140                           

aaa ttg tat gca aag aag aaa ccc gag tga                                462
Lys Leu Tyr Ala Lys Lys Lys Pro Glu                                       
145                 150                                                   


<210>  14
<211>  153
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  14

Met Ala Ser Leu Pro Lys Arg Ile Ile Lys Glu Thr Glu Lys Leu Val 
1               5                   10                  15      


Ser Asp Pro Val Pro Gly Ile Thr Ala Glu Pro His Asp Asp Asn Leu 
            20                  25                  30          


Arg Tyr Phe Gln Val Thr Ile Glu Gly Pro Glu Gln Ser Pro Tyr Glu 
        35                  40                  45              


Asp Gly Ile Phe Glu Leu Glu Leu Tyr Leu Pro Asp Asp Tyr Pro Met 
    50                  55                  60                  


Glu Ala Pro Lys Val Arg Phe Leu Thr Lys Ile Tyr His Pro Asn Ile 
65                  70                  75                  80  


Asp Arg Leu Gly Arg Ile Cys Leu Asp Val Leu Lys Thr Asn Trp Ser 
                85                  90                  95      


Pro Ala Leu Gln Ile Arg Thr Val Leu Leu Ser Ile Gln Ala Leu Leu 
            100                 105                 110         


Ala Ser Pro Asn Pro Asn Asp Pro Leu Ala Asn Asp Val Ala Glu Asp 
        115                 120                 125             


Trp Ile Lys Asn Glu Gln Gly Ala Lys Ala Lys Ala Arg Glu Trp Thr 
    130                 135                 140                 


Lys Leu Tyr Ala Lys Lys Lys Pro Glu 
145                 150             


<210>  15
<211>  461
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(216)
<223>  UBC13_1 mutant gene (209deletedG) encoding truncated Ubc13_1 
       protein.

<400>  15
atg gca tca tta ccc aag aga ata atc aag gaa act gag aaa tta gta         48
Met Ala Ser Leu Pro Lys Arg Ile Ile Lys Glu Thr Glu Lys Leu Val           
1               5                   10                  15                

agt gac cca gta cct ggc att aca gca gaa cca cat gac gac aac tta         96
Ser Asp Pro Val Pro Gly Ile Thr Ala Glu Pro His Asp Asp Asn Leu           
            20                  25                  30                    

cgt tac ttc caa gta aca atc gaa ggc cca gaa cag tca cca tat gaa        144
Arg Tyr Phe Gln Val Thr Ile Glu Gly Pro Glu Gln Ser Pro Tyr Glu           
        35                  40                  45                        

gac ggt att ttc gaa tta gaa tta tat ctg cct gac gac tat cca atg        192
Asp Gly Ile Phe Glu Leu Glu Leu Tyr Leu Pro Asp Asp Tyr Pro Met           
    50                  55                  60                            

gaa gca ccg aaa gta ctt ttt taa ccaagatata tcatcccaat attgataggt       246
Glu Ala Pro Lys Val Leu Phe                                               
65                  70                                                    

tgggccgtat atgtcttgat gtgcttaaaa ctaattggtc acccgcttta cagatcagaa      306

ccgtcctgct gtccattcaa gcactgttgg ctagccctaa tcctaatgat ccgctagcta      366

atgacgtcgc tgaagattgg ataaagaatg agcaaggtgc caaagctaag gctcgcgaat      426

ggacgaaatt gtatgcaaag aagaaacccg agtga                                 461


<210>  16
<211>  71
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  16

Met Ala Ser Leu Pro Lys Arg Ile Ile Lys Glu Thr Glu Lys Leu Val 
1               5                   10                  15      


Ser Asp Pro Val Pro Gly Ile Thr Ala Glu Pro His Asp Asp Asn Leu 
            20                  25                  30          


Arg Tyr Phe Gln Val Thr Ile Glu Gly Pro Glu Gln Ser Pro Tyr Glu 
        35                  40                  45              


Asp Gly Ile Phe Glu Leu Glu Leu Tyr Leu Pro Asp Asp Tyr Pro Met 
    50                  55                  60                  


Glu Ala Pro Lys Val Leu Phe 
65                  70      


<210>  17
<211>  4470
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(4470)
<223>  INO80 mutant  gene (G1076A) encoding a mutant Ino80 protein 
       (C359Y)

<400>  17
atg tca ctg gca gtt cta ctc aat aag gag gac aag gat ata tct gat         48
Met Ser Leu Ala Val Leu Leu Asn Lys Glu Asp Lys Asp Ile Ser Asp           
1               5                   10                  15                

ttt tca aag act acc gca ggc aag tct gct aag aag aat agc aga gag         96
Phe Ser Lys Thr Thr Ala Gly Lys Ser Ala Lys Lys Asn Ser Arg Glu           
            20                  25                  30                    

aga gtt gcc gat gtg gcg ccc acc aga gtg cta gat aag aaa caa gcg        144
Arg Val Ala Asp Val Ala Pro Thr Arg Val Leu Asp Lys Lys Gln Ala           
        35                  40                  45                        

tat cta tct caa ctg aac agt gag ttc aac cgt atc aag aga agg gat        192
Tyr Leu Ser Gln Leu Asn Ser Glu Phe Asn Arg Ile Lys Arg Arg Asp           
    50                  55                  60                            

tcg ata gag caa ctt tat cag gac tgg aaa ttt atc aat cta caa gag        240
Ser Ile Glu Gln Leu Tyr Gln Asp Trp Lys Phe Ile Asn Leu Gln Glu           
65                  70                  75                  80            

ttc gag ttg ata tcg gaa tgg aat caa caa tcc aag gat tgg caa ttt        288
Phe Glu Leu Ile Ser Glu Trp Asn Gln Gln Ser Lys Asp Trp Gln Phe           
                85                  90                  95                

gat aac act aat gat tct cag gac ctg cat ttt aaa aaa ctg tac cgt        336
Asp Asn Thr Asn Asp Ser Gln Asp Leu His Phe Lys Lys Leu Tyr Arg           
            100                 105                 110                   

gat atg agc atg atc aat aaa gaa tgg gcg gaa tac caa tct ttt aaa        384
Asp Met Ser Met Ile Asn Lys Glu Trp Ala Glu Tyr Gln Ser Phe Lys           
        115                 120                 125                       

aat gca aat tta tcg gac ata atc aat gaa aag gac gct gac gag gat        432
Asn Ala Asn Leu Ser Asp Ile Ile Asn Glu Lys Asp Ala Asp Glu Asp           
    130                 135                 140                           

gaa gaa gat gat gaa gac gag ctt gaa gat ggt gaa gaa gac atg gaa        480
Glu Glu Asp Asp Glu Asp Glu Leu Glu Asp Gly Glu Glu Asp Met Glu           
145                 150                 155                 160           

gaa gat gag gca agt act ggt agg cat acc aat ggg aag agc atg cgt        528
Glu Asp Glu Ala Ser Thr Gly Arg His Thr Asn Gly Lys Ser Met Arg           
                165                 170                 175               

ggg aat ggt att caa aaa tcg aga aag aag gat gct gct gct gct gcc        576
Gly Asn Gly Ile Gln Lys Ser Arg Lys Lys Asp Ala Ala Ala Ala Ala           
            180                 185                 190                   

gct att ggg aaa gcc ata aag gat gat caa act cat gcc gat act gtt        624
Ala Ile Gly Lys Ala Ile Lys Asp Asp Gln Thr His Ala Asp Thr Val           
        195                 200                 205                       

gtt act gtt aat gga gac gaa aac gag gat ggc aat aac ggt gaa gac        672
Val Thr Val Asn Gly Asp Glu Asn Glu Asp Gly Asn Asn Gly Glu Asp           
    210                 215                 220                           

gaa gac aac gac aac gac aat gaa aac aac aac gac aac gac aac gac        720
Glu Asp Asn Asp Asn Asp Asn Glu Asn Asn Asn Asp Asn Asp Asn Asp           
225                 230                 235                 240           

aac gaa aat gaa aat gac aat gac agt gac aat gat gac gag gaa gaa        768
Asn Glu Asn Glu Asn Asp Asn Asp Ser Asp Asn Asp Asp Glu Glu Glu           
                245                 250                 255               

aat gga gag gag gac gaa gag gaa gag gaa atc gag gat ctt gat gaa        816
Asn Gly Glu Glu Asp Glu Glu Glu Glu Glu Ile Glu Asp Leu Asp Glu           
            260                 265                 270                   

gaa gat ttc gcc gcc ttt gaa gaa cag gat gac aat gac gat gag gac        864
Glu Asp Phe Ala Ala Phe Glu Glu Gln Asp Asp Asn Asp Asp Glu Asp           
        275                 280                 285                       

ttt aat ccc gat gtc gag aaa agg aga aag aga tct tcc tct tct tct        912
Phe Asn Pro Asp Val Glu Lys Arg Arg Lys Arg Ser Ser Ser Ser Ser           
    290                 295                 300                           

tca tcc acc aaa cta tct atg aac tcg ctg tct tta ata acg tcc aag        960
Ser Ser Thr Lys Leu Ser Met Asn Ser Leu Ser Leu Ile Thr Ser Lys           
305                 310                 315                 320           

aaa ata aat aaa aat att acc atc aac agt gat agg ccg aag att gta       1008
Lys Ile Asn Lys Asn Ile Thr Ile Asn Ser Asp Arg Pro Lys Ile Val           
                325                 330                 335               

cgt gaa ctt atc aaa atg tgc aac aaa aac aag cat cag aag att aag       1056
Arg Glu Leu Ile Lys Met Cys Asn Lys Asn Lys His Gln Lys Ile Lys           
            340                 345                 350                   

aag agg agg ttc acc aat tat att gtc acc gac tac aat cca atc gat       1104
Lys Arg Arg Phe Thr Asn Tyr Ile Val Thr Asp Tyr Asn Pro Ile Asp           
        355                 360                 365                       

tcc aag ttg aac atc aaa ata act ttg aaa cag tac cac gtc aaa agg       1152
Ser Lys Leu Asn Ile Lys Ile Thr Leu Lys Gln Tyr His Val Lys Arg           
    370                 375                 380                           

ttg aaa aaa ctg att aac gat gcg aag aga gaa aga gaa agg gaa gag       1200
Leu Lys Lys Leu Ile Asn Asp Ala Lys Arg Glu Arg Glu Arg Glu Glu           
385                 390                 395                 400           

gcc ttg aag aac aac gtt ggt ttg gat ggt aat gat ttg gat aat gat       1248
Ala Leu Lys Asn Asn Val Gly Leu Asp Gly Asn Asp Leu Asp Asn Asp           
                405                 410                 415               

gag gat gga tct gaa tcc cac aag aga agg aaa tta aac aat aat act       1296
Glu Asp Gly Ser Glu Ser His Lys Arg Arg Lys Leu Asn Asn Asn Thr           
            420                 425                 430                   

gct aac ggt gcc gat gat gct aat aag aga aaa ttc aac act aga cac       1344
Ala Asn Gly Ala Asp Asp Ala Asn Lys Arg Lys Phe Asn Thr Arg His           
        435                 440                 445                       

ggg ttg ccc act tac ggc atg aaa atg aat gct aag gaa gcc cgt gct       1392
Gly Leu Pro Thr Tyr Gly Met Lys Met Asn Ala Lys Glu Ala Arg Ala           
    450                 455                 460                           

atc cag agg cat tac gac aac acc tat aca act att tgg aaa gac atg       1440
Ile Gln Arg His Tyr Asp Asn Thr Tyr Thr Thr Ile Trp Lys Asp Met           
465                 470                 475                 480           

gct cgt aag gat tct acg aaa atg tca aga ttg gtt cag caa ata caa       1488
Ala Arg Lys Asp Ser Thr Lys Met Ser Arg Leu Val Gln Gln Ile Gln           
                485                 490                 495               

tca atc aga tct aca aat ttt agg aaa act tcc tca ctt tgt gct aga       1536
Ser Ile Arg Ser Thr Asn Phe Arg Lys Thr Ser Ser Leu Cys Ala Arg           
            500                 505                 510                   

gaa gcc aag aaa tgg caa tcc aag aac ttc aaa caa ata aag gac ttc       1584
Glu Ala Lys Lys Trp Gln Ser Lys Asn Phe Lys Gln Ile Lys Asp Phe           
        515                 520                 525                       

caa acg agg gcc aga aga ggt att agg gaa atg tcc aat ttt tgg aag       1632
Gln Thr Arg Ala Arg Arg Gly Ile Arg Glu Met Ser Asn Phe Trp Lys           
    530                 535                 540                           

aag aat gag cgt gaa gaa aga gat cta aag aag aag ata gaa aag gag       1680
Lys Asn Glu Arg Glu Glu Arg Asp Leu Lys Lys Lys Ile Glu Lys Glu           
545                 550                 555                 560           

gct atg gaa cag gca aag aag gaa gaa gag gag aag gaa tcc aaa aga       1728
Ala Met Glu Gln Ala Lys Lys Glu Glu Glu Glu Lys Glu Ser Lys Arg           
                565                 570                 575               

caa gct aaa aag ctg aac ttt ttg tta aca cag aca gaa ttg tat tcg       1776
Gln Ala Lys Lys Leu Asn Phe Leu Leu Thr Gln Thr Glu Leu Tyr Ser           
            580                 585                 590                   

cat ttc att gga agg aaa atc aaa act aac gaa ttg gaa ggg aac aat       1824
His Phe Ile Gly Arg Lys Ile Lys Thr Asn Glu Leu Glu Gly Asn Asn           
        595                 600                 605                       

gtt tcc agt aat gat tct gaa agc cag aag aat att gac att tca gct       1872
Val Ser Ser Asn Asp Ser Glu Ser Gln Lys Asn Ile Asp Ile Ser Ala           
    610                 615                 620                           

ttg gct cca aat aaa aat gac ttt cac gcc att gac ttt gat aac gaa       1920
Leu Ala Pro Asn Lys Asn Asp Phe His Ala Ile Asp Phe Asp Asn Glu           
625                 630                 635                 640           

aat gac gaa caa ttg aga cta aga gct gct gaa aat gct tcc aat gca       1968
Asn Asp Glu Gln Leu Arg Leu Arg Ala Ala Glu Asn Ala Ser Asn Ala           
                645                 650                 655               

ctg gca gaa aca aga gcg aaa gcg aaa caa ttt gat gat cat gcc aat       2016
Leu Ala Glu Thr Arg Ala Lys Ala Lys Gln Phe Asp Asp His Ala Asn           
            660                 665                 670                   

gcg cat gag gaa gaa gag gag gaa gat gag ttg aac ttc caa aac cca       2064
Ala His Glu Glu Glu Glu Glu Glu Asp Glu Leu Asn Phe Gln Asn Pro           
        675                 680                 685                       

acc tct tta ggt gaa ata acc atc gag caa cca aag att ttg gca tgt       2112
Thr Ser Leu Gly Glu Ile Thr Ile Glu Gln Pro Lys Ile Leu Ala Cys           
    690                 695                 700                           

act ttg aaa gaa tat cag ctc aag ggt ttg aat tgg cta gct aac ctt       2160
Thr Leu Lys Glu Tyr Gln Leu Lys Gly Leu Asn Trp Leu Ala Asn Leu           
705                 710                 715                 720           

tac gac cag ggt atc aat ggt atc tta gca gat gaa atg ggg tta ggt       2208
Tyr Asp Gln Gly Ile Asn Gly Ile Leu Ala Asp Glu Met Gly Leu Gly           
                725                 730                 735               

aaa act gtg caa tct att tcc gtt ttg gct cat cta gcc gag aac cat       2256
Lys Thr Val Gln Ser Ile Ser Val Leu Ala His Leu Ala Glu Asn His           
            740                 745                 750                   

aat att tgg ggg ccc ttt ttg gtg gtc aca ccc gct tct act ctg cat       2304
Asn Ile Trp Gly Pro Phe Leu Val Val Thr Pro Ala Ser Thr Leu His           
        755                 760                 765                       

aac tgg gtc aat gaa ata tcc aaa ttt ttg cct caa ttt aaa att ctg       2352
Asn Trp Val Asn Glu Ile Ser Lys Phe Leu Pro Gln Phe Lys Ile Leu           
    770                 775                 780                           

cca tac tgg ggt aat gcc aat gac cgt aaa gtt ctt agg aaa ttt tgg       2400
Pro Tyr Trp Gly Asn Ala Asn Asp Arg Lys Val Leu Arg Lys Phe Trp           
785                 790                 795                 800           

gac agg aaa aat ctg agg tat aat aag aat gcg cca ttc cat gtc atg       2448
Asp Arg Lys Asn Leu Arg Tyr Asn Lys Asn Ala Pro Phe His Val Met           
                805                 810                 815               

gtt aca tcc tat caa atg gtt gtc act gat gct aac tat ttg cag aaa       2496
Val Thr Ser Tyr Gln Met Val Val Thr Asp Ala Asn Tyr Leu Gln Lys           
            820                 825                 830                   

atg aaa tgg caa tat atg ata ctg gat gag gcc caa gct atc aaa tct       2544
Met Lys Trp Gln Tyr Met Ile Leu Asp Glu Ala Gln Ala Ile Lys Ser           
        835                 840                 845                       

tca cag tca tct aga tgg aag aac ctg ttg agt ttt cat tgt cgt aat       2592
Ser Gln Ser Ser Arg Trp Lys Asn Leu Leu Ser Phe His Cys Arg Asn           
    850                 855                 860                           

aga tta cta tta acg ggt act cct atc caa aac agt atg cag gaa cta       2640
Arg Leu Leu Leu Thr Gly Thr Pro Ile Gln Asn Ser Met Gln Glu Leu           
865                 870                 875                 880           

tgg gca ctt tta cat ttt atc atg cct tct ctt ttt gat tct cac gat       2688
Trp Ala Leu Leu His Phe Ile Met Pro Ser Leu Phe Asp Ser His Asp           
                885                 890                 895               

gaa ttt aat gaa tgg ttc tct aag gat att gaa tct cat gca gaa gct       2736
Glu Phe Asn Glu Trp Phe Ser Lys Asp Ile Glu Ser His Ala Glu Ala           
            900                 905                 910                   

aat acc aaa tta aac caa cag caa tta cgt cgt ttg cat atg att ttg       2784
Asn Thr Lys Leu Asn Gln Gln Gln Leu Arg Arg Leu His Met Ile Leu           
        915                 920                 925                       

aaa ccg ttc atg tta agg cgt gtc aaa aag aac gtc caa tca gaa ttg       2832
Lys Pro Phe Met Leu Arg Arg Val Lys Lys Asn Val Gln Ser Glu Leu           
    930                 935                 940                           

ggc gac aag att gaa att gat gtc tta tgt gat tta act caa agg cag       2880
Gly Asp Lys Ile Glu Ile Asp Val Leu Cys Asp Leu Thr Gln Arg Gln           
945                 950                 955                 960           

gct aaa tta tat cag gtt ttg aaa tct caa atc tct act aac tat gat       2928
Ala Lys Leu Tyr Gln Val Leu Lys Ser Gln Ile Ser Thr Asn Tyr Asp           
                965                 970                 975               

gcc att gag aat gcc gct acg aat gat tct act tcc aat tct gct tca       2976
Ala Ile Glu Asn Ala Ala Thr Asn Asp Ser Thr Ser Asn Ser Ala Ser           
            980                 985                 990                   

aat tct ggt tct gat cag aac ttg  att aac gca gtc atg  caa ttc agg     3024
Asn Ser Gly Ser Asp Gln Asn Leu  Ile Asn Ala Val Met  Gln Phe Arg         
        995                 1000                 1005                     

aaa gtt  tgt aac cac ccg gat  ctt ttt gaa agg gca  gat gta gat        3069
Lys Val  Cys Asn His Pro Asp  Leu Phe Glu Arg Ala  Asp Val Asp            
    1010                 1015                 1020                        

tct cca  ttt tcc ttc act act  ttt ggt aaa act acc  tcg atg tta        3114
Ser Pro  Phe Ser Phe Thr Thr  Phe Gly Lys Thr Thr  Ser Met Leu            
    1025                 1030                 1035                        

act gca  tct gtt gct aat aac  aac agc agc gta att  agc aac agt        3159
Thr Ala  Ser Val Ala Asn Asn  Asn Ser Ser Val Ile  Ser Asn Ser            
    1040                 1045                 1050                        

aat atg  aat ctc tct agc atg  agc agt aat aat att  tcc aat ggg        3204
Asn Met  Asn Leu Ser Ser Met  Ser Ser Asn Asn Ile  Ser Asn Gly            
    1055                 1060                 1065                        

aaa ttc  act gat ttg att tac  tct tca agg aat cca  ata aaa tat        3249
Lys Phe  Thr Asp Leu Ile Tyr  Ser Ser Arg Asn Pro  Ile Lys Tyr            
    1070                 1075                 1080                        

agc cta  cca agg ttg att tat  gaa gat ttg att tta  ccg aat tac        3294
Ser Leu  Pro Arg Leu Ile Tyr  Glu Asp Leu Ile Leu  Pro Asn Tyr            
    1085                 1090                 1095                        

aac aat  gac gtg gac ata gcc  aat aaa ctg aag aac  gtg aaa ttt        3339
Asn Asn  Asp Val Asp Ile Ala  Asn Lys Leu Lys Asn  Val Lys Phe            
    1100                 1105                 1110                        

aat att  ttc aac cca tca act  aat tat gaa ctg tgc  tta ttt tta        3384
Asn Ile  Phe Asn Pro Ser Thr  Asn Tyr Glu Leu Cys  Leu Phe Leu            
    1115                 1120                 1125                        

tct aaa  tta aca ggt gaa ccc  tcg ttg aat gaa ttt  ttt aga gtt        3429
Ser Lys  Leu Thr Gly Glu Pro  Ser Leu Asn Glu Phe  Phe Arg Val            
    1130                 1135                 1140                        

tct act  aca ccc tta ctc aag  cga gta ata gaa cgt  aca aat ggt        3474
Ser Thr  Thr Pro Leu Leu Lys  Arg Val Ile Glu Arg  Thr Asn Gly            
    1145                 1150                 1155                        

cca aaa  aat act gac agt tta  agt ttt aaa acc att  act caa gag        3519
Pro Lys  Asn Thr Asp Ser Leu  Ser Phe Lys Thr Ile  Thr Gln Glu            
    1160                 1165                 1170                        

cta ctc  gag gtt act aga aat  gca cct tcc gag ggc  gta atg gct        3564
Leu Leu  Glu Val Thr Arg Asn  Ala Pro Ser Glu Gly  Val Met Ala            
    1175                 1180                 1185                        

tca tta  ttg aac gtt gaa aaa  cat gcc tat gaa cgt  gag tat ctg        3609
Ser Leu  Leu Asn Val Glu Lys  His Ala Tyr Glu Arg  Glu Tyr Leu            
    1190                 1195                 1200                        

aac tgt  ata caa cgt ggt tat  cat ccc aat gtt tca  gct cct ccg        3654
Asn Cys  Ile Gln Arg Gly Tyr  His Pro Asn Val Ser  Ala Pro Pro            
    1205                 1210                 1215                        

gtc acc  att gaa gtt ctt ggt  tct agt cat gtt acc  aat agc ata        3699
Val Thr  Ile Glu Val Leu Gly  Ser Ser His Val Thr  Asn Ser Ile            
    1220                 1225                 1230                        

aat aat  gaa ctg ttt gac ccg  ttg ata agt cag gca  tta tcc gat        3744
Asn Asn  Glu Leu Phe Asp Pro  Leu Ile Ser Gln Ala  Leu Ser Asp            
    1235                 1240                 1245                        

att cca  gca ata acg caa tat  aac atg cac gtt aaa  aag gga ata        3789
Ile Pro  Ala Ile Thr Gln Tyr  Asn Met His Val Lys  Lys Gly Ile            
    1250                 1255                 1260                        

cct gtt  gaa gat ttt cca aag  aca gga cta ttc cct  gaa cca cta        3834
Pro Val  Glu Asp Phe Pro Lys  Thr Gly Leu Phe Pro  Glu Pro Leu            
    1265                 1270                 1275                        

aat aaa  aat ttt tca tcc aac  ata tca atg cca tct  atg gat agg        3879
Asn Lys  Asn Phe Ser Ser Asn  Ile Ser Met Pro Ser  Met Asp Arg            
    1280                 1285                 1290                        

ttc att  acc gaa tca gcg aaa  tta aga aaa ttg gat  gaa ttg ttg        3924
Phe Ile  Thr Glu Ser Ala Lys  Leu Arg Lys Leu Asp  Glu Leu Leu            
    1295                 1300                 1305                        

gta aag  ttg aaa tca gaa ggc  cac aga gtt ttg ata  tat ttc caa        3969
Val Lys  Leu Lys Ser Glu Gly  His Arg Val Leu Ile  Tyr Phe Gln            
    1310                 1315                 1320                        

atg acc  aag atg atg gat ctt  atg gaa gaa tac ttg  acc tat aga        4014
Met Thr  Lys Met Met Asp Leu  Met Glu Glu Tyr Leu  Thr Tyr Arg            
    1325                 1330                 1335                        

caa tat  aat cat att aga ctg  gat ggt tcc tca aag  ttg gag gac        4059
Gln Tyr  Asn His Ile Arg Leu  Asp Gly Ser Ser Lys  Leu Glu Asp            
    1340                 1345                 1350                        

cgt cga  gat ttg gtc cat gat  tgg cag act aat cca  gaa atc ttt        4104
Arg Arg  Asp Leu Val His Asp  Trp Gln Thr Asn Pro  Glu Ile Phe            
    1355                 1360                 1365                        

gtt ttc  ctc cta agt aca aga  gca ggt ggt cta ggt  atc aac ctg        4149
Val Phe  Leu Leu Ser Thr Arg  Ala Gly Gly Leu Gly  Ile Asn Leu            
    1370                 1375                 1380                        

acc gca  gct gac act gtt ata  ttc tat gat tca gat  tgg aac cct        4194
Thr Ala  Ala Asp Thr Val Ile  Phe Tyr Asp Ser Asp  Trp Asn Pro            
    1385                 1390                 1395                        

act att  gat tca cag gct atg  gat agg gca cat agg  ttg ggc caa        4239
Thr Ile  Asp Ser Gln Ala Met  Asp Arg Ala His Arg  Leu Gly Gln            
    1400                 1405                 1410                        

acg agg  caa gtc aca gtg tac  aga tta ctt gtt cgt  ggt acc atc        4284
Thr Arg  Gln Val Thr Val Tyr  Arg Leu Leu Val Arg  Gly Thr Ile            
    1415                 1420                 1425                        

gaa gaa  aga atg agg gat aga  gca aag caa aaa gag  caa gtt caa        4329
Glu Glu  Arg Met Arg Asp Arg  Ala Lys Gln Lys Glu  Gln Val Gln            
    1430                 1435                 1440                        

caa gtc  gtc atg gaa ggt aag  act cag gaa aaa aac  att aaa acc        4374
Gln Val  Val Met Glu Gly Lys  Thr Gln Glu Lys Asn  Ile Lys Thr            
    1445                 1450                 1455                        

att gaa  gtg ggt gaa aat gat  tcc gaa gtc act cgt  gaa ggt agc        4419
Ile Glu  Val Gly Glu Asn Asp  Ser Glu Val Thr Arg  Glu Gly Ser            
    1460                 1465                 1470                        

aaa agc  ata agt caa gat gga  att aag gaa gcg gca  agt gca ttg        4464
Lys Ser  Ile Ser Gln Asp Gly  Ile Lys Glu Ala Ala  Ser Ala Leu            
    1475                 1480                 1485                        

gca tga                                                               4470
Ala                                                                       
                                                                          


<210>  18
<211>  1489
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  18

Met Ser Leu Ala Val Leu Leu Asn Lys Glu Asp Lys Asp Ile Ser Asp 
1               5                   10                  15      


Phe Ser Lys Thr Thr Ala Gly Lys Ser Ala Lys Lys Asn Ser Arg Glu 
            20                  25                  30          


Arg Val Ala Asp Val Ala Pro Thr Arg Val Leu Asp Lys Lys Gln Ala 
        35                  40                  45              


Tyr Leu Ser Gln Leu Asn Ser Glu Phe Asn Arg Ile Lys Arg Arg Asp 
    50                  55                  60                  


Ser Ile Glu Gln Leu Tyr Gln Asp Trp Lys Phe Ile Asn Leu Gln Glu 
65                  70                  75                  80  


Phe Glu Leu Ile Ser Glu Trp Asn Gln Gln Ser Lys Asp Trp Gln Phe 
                85                  90                  95      


Asp Asn Thr Asn Asp Ser Gln Asp Leu His Phe Lys Lys Leu Tyr Arg 
            100                 105                 110         


Asp Met Ser Met Ile Asn Lys Glu Trp Ala Glu Tyr Gln Ser Phe Lys 
        115                 120                 125             


Asn Ala Asn Leu Ser Asp Ile Ile Asn Glu Lys Asp Ala Asp Glu Asp 
    130                 135                 140                 


Glu Glu Asp Asp Glu Asp Glu Leu Glu Asp Gly Glu Glu Asp Met Glu 
145                 150                 155                 160 


Glu Asp Glu Ala Ser Thr Gly Arg His Thr Asn Gly Lys Ser Met Arg 
                165                 170                 175     


Gly Asn Gly Ile Gln Lys Ser Arg Lys Lys Asp Ala Ala Ala Ala Ala 
            180                 185                 190         


Ala Ile Gly Lys Ala Ile Lys Asp Asp Gln Thr His Ala Asp Thr Val 
        195                 200                 205             


Val Thr Val Asn Gly Asp Glu Asn Glu Asp Gly Asn Asn Gly Glu Asp 
    210                 215                 220                 


Glu Asp Asn Asp Asn Asp Asn Glu Asn Asn Asn Asp Asn Asp Asn Asp 
225                 230                 235                 240 


Asn Glu Asn Glu Asn Asp Asn Asp Ser Asp Asn Asp Asp Glu Glu Glu 
                245                 250                 255     


Asn Gly Glu Glu Asp Glu Glu Glu Glu Glu Ile Glu Asp Leu Asp Glu 
            260                 265                 270         


Glu Asp Phe Ala Ala Phe Glu Glu Gln Asp Asp Asn Asp Asp Glu Asp 
        275                 280                 285             


Phe Asn Pro Asp Val Glu Lys Arg Arg Lys Arg Ser Ser Ser Ser Ser 
    290                 295                 300                 


Ser Ser Thr Lys Leu Ser Met Asn Ser Leu Ser Leu Ile Thr Ser Lys 
305                 310                 315                 320 


Lys Ile Asn Lys Asn Ile Thr Ile Asn Ser Asp Arg Pro Lys Ile Val 
                325                 330                 335     


Arg Glu Leu Ile Lys Met Cys Asn Lys Asn Lys His Gln Lys Ile Lys 
            340                 345                 350         


Lys Arg Arg Phe Thr Asn Tyr Ile Val Thr Asp Tyr Asn Pro Ile Asp 
        355                 360                 365             


Ser Lys Leu Asn Ile Lys Ile Thr Leu Lys Gln Tyr His Val Lys Arg 
    370                 375                 380                 


Leu Lys Lys Leu Ile Asn Asp Ala Lys Arg Glu Arg Glu Arg Glu Glu 
385                 390                 395                 400 


Ala Leu Lys Asn Asn Val Gly Leu Asp Gly Asn Asp Leu Asp Asn Asp 
                405                 410                 415     


Glu Asp Gly Ser Glu Ser His Lys Arg Arg Lys Leu Asn Asn Asn Thr 
            420                 425                 430         


Ala Asn Gly Ala Asp Asp Ala Asn Lys Arg Lys Phe Asn Thr Arg His 
        435                 440                 445             


Gly Leu Pro Thr Tyr Gly Met Lys Met Asn Ala Lys Glu Ala Arg Ala 
    450                 455                 460                 


Ile Gln Arg His Tyr Asp Asn Thr Tyr Thr Thr Ile Trp Lys Asp Met 
465                 470                 475                 480 


Ala Arg Lys Asp Ser Thr Lys Met Ser Arg Leu Val Gln Gln Ile Gln 
                485                 490                 495     


Ser Ile Arg Ser Thr Asn Phe Arg Lys Thr Ser Ser Leu Cys Ala Arg 
            500                 505                 510         


Glu Ala Lys Lys Trp Gln Ser Lys Asn Phe Lys Gln Ile Lys Asp Phe 
        515                 520                 525             


Gln Thr Arg Ala Arg Arg Gly Ile Arg Glu Met Ser Asn Phe Trp Lys 
    530                 535                 540                 


Lys Asn Glu Arg Glu Glu Arg Asp Leu Lys Lys Lys Ile Glu Lys Glu 
545                 550                 555                 560 


Ala Met Glu Gln Ala Lys Lys Glu Glu Glu Glu Lys Glu Ser Lys Arg 
                565                 570                 575     


Gln Ala Lys Lys Leu Asn Phe Leu Leu Thr Gln Thr Glu Leu Tyr Ser 
            580                 585                 590         


His Phe Ile Gly Arg Lys Ile Lys Thr Asn Glu Leu Glu Gly Asn Asn 
        595                 600                 605             


Val Ser Ser Asn Asp Ser Glu Ser Gln Lys Asn Ile Asp Ile Ser Ala 
    610                 615                 620                 


Leu Ala Pro Asn Lys Asn Asp Phe His Ala Ile Asp Phe Asp Asn Glu 
625                 630                 635                 640 


Asn Asp Glu Gln Leu Arg Leu Arg Ala Ala Glu Asn Ala Ser Asn Ala 
                645                 650                 655     


Leu Ala Glu Thr Arg Ala Lys Ala Lys Gln Phe Asp Asp His Ala Asn 
            660                 665                 670         


Ala His Glu Glu Glu Glu Glu Glu Asp Glu Leu Asn Phe Gln Asn Pro 
        675                 680                 685             


Thr Ser Leu Gly Glu Ile Thr Ile Glu Gln Pro Lys Ile Leu Ala Cys 
    690                 695                 700                 


Thr Leu Lys Glu Tyr Gln Leu Lys Gly Leu Asn Trp Leu Ala Asn Leu 
705                 710                 715                 720 


Tyr Asp Gln Gly Ile Asn Gly Ile Leu Ala Asp Glu Met Gly Leu Gly 
                725                 730                 735     


Lys Thr Val Gln Ser Ile Ser Val Leu Ala His Leu Ala Glu Asn His 
            740                 745                 750         


Asn Ile Trp Gly Pro Phe Leu Val Val Thr Pro Ala Ser Thr Leu His 
        755                 760                 765             


Asn Trp Val Asn Glu Ile Ser Lys Phe Leu Pro Gln Phe Lys Ile Leu 
    770                 775                 780                 


Pro Tyr Trp Gly Asn Ala Asn Asp Arg Lys Val Leu Arg Lys Phe Trp 
785                 790                 795                 800 


Asp Arg Lys Asn Leu Arg Tyr Asn Lys Asn Ala Pro Phe His Val Met 
                805                 810                 815     


Val Thr Ser Tyr Gln Met Val Val Thr Asp Ala Asn Tyr Leu Gln Lys 
            820                 825                 830         


Met Lys Trp Gln Tyr Met Ile Leu Asp Glu Ala Gln Ala Ile Lys Ser 
        835                 840                 845             


Ser Gln Ser Ser Arg Trp Lys Asn Leu Leu Ser Phe His Cys Arg Asn 
    850                 855                 860                 


Arg Leu Leu Leu Thr Gly Thr Pro Ile Gln Asn Ser Met Gln Glu Leu 
865                 870                 875                 880 


Trp Ala Leu Leu His Phe Ile Met Pro Ser Leu Phe Asp Ser His Asp 
                885                 890                 895     


Glu Phe Asn Glu Trp Phe Ser Lys Asp Ile Glu Ser His Ala Glu Ala 
            900                 905                 910         


Asn Thr Lys Leu Asn Gln Gln Gln Leu Arg Arg Leu His Met Ile Leu 
        915                 920                 925             


Lys Pro Phe Met Leu Arg Arg Val Lys Lys Asn Val Gln Ser Glu Leu 
    930                 935                 940                 


Gly Asp Lys Ile Glu Ile Asp Val Leu Cys Asp Leu Thr Gln Arg Gln 
945                 950                 955                 960 


Ala Lys Leu Tyr Gln Val Leu Lys Ser Gln Ile Ser Thr Asn Tyr Asp 
                965                 970                 975     


Ala Ile Glu Asn Ala Ala Thr Asn Asp Ser Thr Ser Asn Ser Ala Ser 
            980                 985                 990         


Asn Ser Gly Ser Asp Gln Asn Leu  Ile Asn Ala Val Met  Gln Phe Arg 
        995                 1000                 1005             


Lys Val  Cys Asn His Pro Asp  Leu Phe Glu Arg Ala  Asp Val Asp 
    1010                 1015                 1020             


Ser Pro  Phe Ser Phe Thr Thr  Phe Gly Lys Thr Thr  Ser Met Leu 
    1025                 1030                 1035             


Thr Ala  Ser Val Ala Asn Asn  Asn Ser Ser Val Ile  Ser Asn Ser 
    1040                 1045                 1050             


Asn Met  Asn Leu Ser Ser Met  Ser Ser Asn Asn Ile  Ser Asn Gly 
    1055                 1060                 1065             


Lys Phe  Thr Asp Leu Ile Tyr  Ser Ser Arg Asn Pro  Ile Lys Tyr 
    1070                 1075                 1080             


Ser Leu  Pro Arg Leu Ile Tyr  Glu Asp Leu Ile Leu  Pro Asn Tyr 
    1085                 1090                 1095             


Asn Asn  Asp Val Asp Ile Ala  Asn Lys Leu Lys Asn  Val Lys Phe 
    1100                 1105                 1110             


Asn Ile  Phe Asn Pro Ser Thr  Asn Tyr Glu Leu Cys  Leu Phe Leu 
    1115                 1120                 1125             


Ser Lys  Leu Thr Gly Glu Pro  Ser Leu Asn Glu Phe  Phe Arg Val 
    1130                 1135                 1140             


Ser Thr  Thr Pro Leu Leu Lys  Arg Val Ile Glu Arg  Thr Asn Gly 
    1145                 1150                 1155             


Pro Lys  Asn Thr Asp Ser Leu  Ser Phe Lys Thr Ile  Thr Gln Glu 
    1160                 1165                 1170             


Leu Leu  Glu Val Thr Arg Asn  Ala Pro Ser Glu Gly  Val Met Ala 
    1175                 1180                 1185             


Ser Leu  Leu Asn Val Glu Lys  His Ala Tyr Glu Arg  Glu Tyr Leu 
    1190                 1195                 1200             


Asn Cys  Ile Gln Arg Gly Tyr  His Pro Asn Val Ser  Ala Pro Pro 
    1205                 1210                 1215             


Val Thr  Ile Glu Val Leu Gly  Ser Ser His Val Thr  Asn Ser Ile 
    1220                 1225                 1230             


Asn Asn  Glu Leu Phe Asp Pro  Leu Ile Ser Gln Ala  Leu Ser Asp 
    1235                 1240                 1245             


Ile Pro  Ala Ile Thr Gln Tyr  Asn Met His Val Lys  Lys Gly Ile 
    1250                 1255                 1260             


Pro Val  Glu Asp Phe Pro Lys  Thr Gly Leu Phe Pro  Glu Pro Leu 
    1265                 1270                 1275             


Asn Lys  Asn Phe Ser Ser Asn  Ile Ser Met Pro Ser  Met Asp Arg 
    1280                 1285                 1290             


Phe Ile  Thr Glu Ser Ala Lys  Leu Arg Lys Leu Asp  Glu Leu Leu 
    1295                 1300                 1305             


Val Lys  Leu Lys Ser Glu Gly  His Arg Val Leu Ile  Tyr Phe Gln 
    1310                 1315                 1320             


Met Thr  Lys Met Met Asp Leu  Met Glu Glu Tyr Leu  Thr Tyr Arg 
    1325                 1330                 1335             


Gln Tyr  Asn His Ile Arg Leu  Asp Gly Ser Ser Lys  Leu Glu Asp 
    1340                 1345                 1350             


Arg Arg  Asp Leu Val His Asp  Trp Gln Thr Asn Pro  Glu Ile Phe 
    1355                 1360                 1365             


Val Phe  Leu Leu Ser Thr Arg  Ala Gly Gly Leu Gly  Ile Asn Leu 
    1370                 1375                 1380             


Thr Ala  Ala Asp Thr Val Ile  Phe Tyr Asp Ser Asp  Trp Asn Pro 
    1385                 1390                 1395             


Thr Ile  Asp Ser Gln Ala Met  Asp Arg Ala His Arg  Leu Gly Gln 
    1400                 1405                 1410             


Thr Arg  Gln Val Thr Val Tyr  Arg Leu Leu Val Arg  Gly Thr Ile 
    1415                 1420                 1425             


Glu Glu  Arg Met Arg Asp Arg  Ala Lys Gln Lys Glu  Gln Val Gln 
    1430                 1435                 1440             


Gln Val  Val Met Glu Gly Lys  Thr Gln Glu Lys Asn  Ile Lys Thr 
    1445                 1450                 1455             


Ile Glu  Val Gly Glu Asn Asp  Ser Glu Val Thr Arg  Glu Gly Ser 
    1460                 1465                 1470             


Lys Ser  Ile Ser Gln Asp Gly  Ile Lys Glu Ala Ala  Ser Ala Leu 
    1475                 1480                 1485             


Ala 
    


<210>  19
<211>  35
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(35)
<223>  PPZ2_1 oligonucleotide

<400>  19
ccaatgttac aagagtctac gttttagagc tagaa                                  35


<210>  20
<211>  35
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(35)
<223>  GUT1_1 oligonucleotide

<400>  20
catccactgc cagaaccgac gttttagagc tagaa                                  35


<210>  21
<211>  35
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(35)
<223>  KGD1_1 oligonucleotide

<400>  21
cggcagcaac agcaccactt gttttagagc tagaa                                  35


<210>  22
<211>  43
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(43)
<223>  UBC13_1 oligonucleotide

<400>  22
tatatcatcc caatattgat gttttagagc tagaaatagc aag                         43


<210>  23
<211>  43
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(43)
<223>  INO80_1 oligonucleotide

<400>  23
ggaatcgatt ggattgtagt gttttagagc tagaaatagc aag                         43


<210>  24
<211>  90
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(90)
<223>  GUT1_1 repair template - positive strand

<400>  24
agacagggac tttttagagg aaatttccga cgtcacatat caaaagtcgc cactgtcggt       60

tctggcggtg gatggcggga tgtcgaggtc                                        90


<210>  25
<211>  90
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(90)
<223>  KGD1 repair template - positive strand

<400>  25
taaggatttc aaggtcagat actgtggtag aaatccaagt ggtgatgttg ctgcaggtag       60

caaatcacta catttggccg aagaagatgc                                        90


<210>  26
<211>  90
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(90)
<223>  UBC13 repair template - positive strand

<400>  26
acgactatcc aatggaagca ccgaaagtac ttttttaacc aagatatatc atcccaatat       60

tgatagattg ggccgtatat gtcttgatgt                                        90


<210>  27
<211>  90
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(90)
<223>  INO80 repair template (positive strand)

<400>  27
caagcatcag aagattaaga agaggaggtt caccaattat attgtcacag actacaatcc       60

aatcgattcc aagttgaaca tcaaaataac                                        90


<210>  28
<211>  20
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  primer_bind
<222>  (1)..(20)
<223>  GUT1F forward primer

<400>  28
tagtcaagag aaacctgccc                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  primer_bind
<222>  (1)..(20)
<223>  GUT1R reverse primer

<400>  29
accttctgac tttgacacag                                                   20


<210>  30
<211>  21
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  primer_bind
<222>  (1)..(21)
<223>  KGD1F forward primer

<400>  30
acccaagata tttcccatct g                                                 21


<210>  31
<211>  23
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  primer_bind
<222>  (1)..(23)
<223>  KGD1R reverse primer

<400>  31
catctttagg attgttggaa aac                                               23


<210>  32
<211>  22
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  primer_bind
<222>  (1)..(22)
<223>  UBC13F forward primer

<400>  32
agtaagtgac ccagtacctg gc                                                22


<210>  33
<211>  21
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  primer_bind
<222>  (1)..(21)
<223>  UBC13R reverse primer

<400>  33
tcactcgggt ttcttctttg c                                                 21


<210>  34
<211>  21
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  primer_bind
<222>  (1)..(21)
<223>  INO80F forward primer

<400>  34
agaacaggat gacaatgacg a                                                 21


<210>  35
<211>  19
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  primer_bind
<222>  (1)..(19)
<223>  INO80R reverse primer

<400>  35
caacccgtgt ctagtgttg                                                    19


