                         SEQUENCE LISTING

<110>  NTxBio, LLC
 
<120>  CELL-FREE EXPRESSION SYSTEM HAVING NOVEL INORGANIC 
       POLYPHOSPHATE-BASED ENERGY REGENERATION

<130>  90125.00011

<150>  62/440,975
<151>  2016-12-30

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  323
<212>  PRT
<213>  Geobacillus Stearothermphilus

<400>  1

Ser Gly Ser Ser Met Ile Glu Ile Glu Lys Pro Lys Ile Glu Thr Val 
1               5                   10                  15      


Glu Leu Ser Glu Asp Ala Lys Tyr Gly Lys Phe Val Val Glu Pro Leu 
            20                  25                  30          


Glu Arg Gly Tyr Gly Thr Thr Leu Gly Asn Ser Leu Arg Arg Ile Leu 
        35                  40                  45              


Leu Ser Ser Leu Pro Gly Ala Ala Val Thr Ser Val Gln Ile Asp Gly 
    50                  55                  60                  


Val Leu His Glu Phe Ser Thr Ile Asp Gly Val Val Glu Asp Val Thr 
65                  70                  75                  80  


Ala Ile Ile Leu Asn Ile Lys Lys Leu Ala Leu Lys Ile Tyr Ser Asp 
                85                  90                  95      


Glu Glu Lys Thr Leu Glu Ile Asp Val Gln Gly Glu Gly Val Val Thr 
            100                 105                 110         


Ala Ala Asp Ile Thr His Asp Ser Asp Val Glu Ile Leu Asn Pro Asp 
        115                 120                 125             


Leu His Ile Ala Thr Leu Ala Glu Gly Gly Arg Leu Arg Met Arg Met 
    130                 135                 140                 


Thr Ala Lys Arg Gly Arg Gly Tyr Val Pro Ala Glu Ala Asn Lys Arg 
145                 150                 155                 160 


Glu Asp Gln Pro Ile Gly Val Ile Pro Ile Asp Ser Ile Tyr Thr Pro 
                165                 170                 175     


Val Ser Arg Val Ser Tyr Gln Val Glu Asn Thr Arg Val Gly Gln Val 
            180                 185                 190         


Thr Asp Tyr Asp Lys Leu Thr Ile Asp Val Trp Thr Asp Gly Ser Ile 
        195                 200                 205             


Gly Pro Lys Glu Ala Ile Ser Leu Gly Ala Lys Ile Leu Thr Glu His 
    210                 215                 220                 


Leu Asn Ile Phe Val Gly Leu Thr Asp Glu Ala Gln Asn Ala Glu Ile 
225                 230                 235                 240 


Met Val Glu Lys Glu Asp Asp Gln Lys Glu Lys Val Leu Glu Met Thr 
                245                 250                 255     


Ile Glu Glu Leu Asp Leu Ser Val Arg Ser Tyr Asn Cys Leu Lys Arg 
            260                 265                 270         


Ala Gly Ile Asn Thr Val Gln Glu Leu Thr Gln Lys Thr Glu Glu Asp 
        275                 280                 285             


Met Met Lys Val Arg Asn Leu Gly Arg Lys Ser Leu Glu Glu Val Lys 
    290                 295                 300                 


Ala Lys Leu Ala Glu Leu Gly Leu Ser Leu Arg Lys Asp Asp Gly Arg 
305                 310                 315                 320 


Arg Ala Ala 
            


<210>  2
<211>  1198
<212>  PRT
<213>  Geobacillus Stearothermphilus

<400>  2

Ser Gly Ser Ser Met Thr Gly Arg Leu Val Gln Tyr Gly Arg His Arg 
1               5                   10                  15      


Gln Arg Arg Ser Tyr Ala Arg Ile Ser Glu Val Leu Glu Leu Pro Asn 
            20                  25                  30          


Leu Ile Glu Ile Gln Thr Ser Ser Tyr Gln Trp Phe Leu Asp Glu Gly 
        35                  40                  45              


Leu Arg Glu Met Phe Arg Glu Ile Ser Pro Ile Glu Asp Phe Ser Gly 
    50                  55                  60                  


Asn Leu Ser Leu Glu Phe Ile Asp Tyr Ser Leu Gly Glu Pro Lys Tyr 
65                  70                  75                  80  


Thr Val Glu Glu Ala Lys Glu Arg Asp Val Thr Tyr Ala Ala Pro Leu 
                85                  90                  95      


Arg Val Lys Val Arg Leu Ile Asn Lys Glu Thr Gly Glu Val Lys Glu 
            100                 105                 110         


Gln Asp Val Phe Met Gly Asp Phe Pro Leu Met Thr Glu Thr Gly Thr 
        115                 120                 125             


Phe Ile Ile Asn Gly Ala Glu Arg Val Ile Val Ser Arg Leu Val Arg 
    130                 135                 140                 


Ser Pro Ser Val Tyr Tyr Ser Asp Lys Val Asp Lys Asn Gly Lys Arg 
145                 150                 155                 160 


Gly Tyr Ser Ala Thr Val Ile Pro Asn Arg Gly Ala Trp Leu Glu Tyr 
                165                 170                 175     


Glu Thr Asp Ala Lys Asp Val Val Tyr Val Arg Ile Asp Arg Thr Arg 
            180                 185                 190         


Lys Leu Pro Val Thr Val Leu Leu Arg Ala Leu Gly Phe Ser Ser Asp 
        195                 200                 205             


Gln Glu Ile Ile Asp Leu Leu Gly Asp Asn Glu Tyr Leu Arg Asn Thr 
    210                 215                 220                 


Leu Glu Lys Asp Asn Thr Asp Ser Thr Glu Lys Ala Leu Ile Glu Ile 
225                 230                 235                 240 


Tyr Glu Arg Leu Arg Pro Gly Glu Pro Pro Thr Leu Glu Asn Ala Lys 
                245                 250                 255     


Asn Leu Leu Ala Ser Arg Phe Phe Asp Pro Lys Arg Tyr Asp Leu Ala 
            260                 265                 270         


Ser Val Gly Arg Tyr Lys Ile Asn Lys Lys Leu His Ile Lys Asn Arg 
        275                 280                 285             


Leu Phe Asn Gln Arg Leu Ala Glu Thr Ile Ile Asp Pro Glu Thr Lys 
    290                 295                 300                 


Glu Val Ile Ala Glu Ala Gly Ala Met Ile Asp Arg Arg Thr Leu Asn 
305                 310                 315                 320 


Arg Leu Leu Pro Tyr Leu Glu Lys Gly Val Gly Leu Gln Thr Tyr Arg 
                325                 330                 335     


Pro Ala Glu Gly Val Val Asp Gly Asp Ile Ser Val Gln Thr Ile Lys 
            340                 345                 350         


Ile Tyr Ala Pro Asn Asp Pro Asp Gly Glu Lys Val Ile Asn Val Ile 
        355                 360                 365             


Gly Asn Gly Phe Ile Ala Glu Asp Val Lys His Ile Thr Pro Ala Asp 
    370                 375                 380                 


Ile Ile Ala Ser Ile Ser Tyr Phe Phe Asn Leu Leu His Gly Val Gly 
385                 390                 395                 400 


Asp Thr Asp Asp Ile Asp His Leu Gly Asn Arg Arg Leu Arg Ser Val 
                405                 410                 415     


Gly Glu Leu Leu Gln Asn Gln Phe Arg Ile Gly Leu Ser Arg Met Glu 
            420                 425                 430         


Arg Val Val Arg Glu Arg Met Ser Ile Gln Asp Thr Asn Thr Ile Thr 
        435                 440                 445             


Pro Gln Gln Leu Ile Asn Ile Arg Pro Val Ile Ala Ala Ile Lys Glu 
    450                 455                 460                 


Phe Phe Gly Ser Ser Gln Leu Ser Gln Phe Met Asp Gln Thr Asn Pro 
465                 470                 475                 480 


Leu Ala Glu Leu Thr His Lys Arg Arg Leu Ser Ala Leu Gly Pro Gly 
                485                 490                 495     


Gly Leu Thr Arg Glu Arg Ala Gly Phe Glu Val Arg Asp Val His Tyr 
            500                 505                 510         


Ser His Tyr Gly Arg Met Cys Pro Ile Glu Thr Pro Glu Gly Pro Asn 
        515                 520                 525             


Ile Gly Leu Ile Asn Ser Leu Ser Thr Tyr Ala Lys Val Asn Lys Phe 
    530                 535                 540                 


Gly Phe Ile Glu Thr Pro Tyr Arg Arg Val Asp Pro Glu Thr Gly Arg 
545                 550                 555                 560 


Val Thr Asp Gln Ile Asp Tyr Leu Thr Ala Asp Glu Glu Asp Asn Tyr 
                565                 570                 575     


Val Val Ala Gln Ala Asn Val Pro Leu Ala Glu Asp Gly Thr Phe Leu 
            580                 585                 590         


Glu Glu Asn Val Val Ala Arg Phe Arg Gly Glu Asn Ile Val Val Lys 
        595                 600                 605             


Arg Asp Arg Val Asp Tyr Met Asp Val Ser Pro Lys Gln Val Val Ser 
    610                 615                 620                 


Ala Ala Thr Ala Cys Ile Pro Phe Leu Glu Asn Asp Asp Ser Asn Arg 
625                 630                 635                 640 


Ala Leu Met Gly Ala Asn Met Gln Arg Gln Ala Val Pro Leu Leu Glu 
                645                 650                 655     


Pro Glu Ala Pro Ile Val Gly Thr Gly Met Glu Tyr Val Ser Ala Lys 
            660                 665                 670         


Asp Ser Gly Ala Ala Val Ile Cys Lys His Arg Gly Ile Val Glu Arg 
        675                 680                 685             


Val Glu Ala Lys Glu Ile Trp Val Arg Arg Leu Ile Glu Val Asp Gly 
    690                 695                 700                 


Lys Glu Val Lys Gly Asp Leu Asp Lys Tyr Arg Leu Leu Lys Phe Val 
705                 710                 715                 720 


Arg Ser Asn Gln Gly Thr Cys Tyr Asn Gln Arg Pro Ile Val Lys Lys 
                725                 730                 735     


Gly Asp Ile Val Glu Lys Gly Glu Ile Leu Ala Asp Gly Pro Ser Met 
            740                 745                 750         


Asp Lys Gly Glu Leu Ala Leu Gly Arg Asn Val Leu Val Ala Phe Met 
        755                 760                 765             


Thr Trp Asp Gly Tyr Asn Tyr Glu Asp Ala Ile Ile Met Ser Glu Arg 
    770                 775                 780                 


Leu Val Lys Glu Asp Val Tyr Thr Ser Ile His Ile Glu Glu Tyr Glu 
785                 790                 795                 800 


Ala Glu Ser Arg Asp Thr Lys Leu Gly Pro Glu Glu Ile Thr Arg Asp 
                805                 810                 815     


Ile Pro Asn Val Gly Glu Asp Ala Leu Lys Asn Leu Asp Glu Arg Gly 
            820                 825                 830         


Ile Val Arg Ile Gly Ala Glu Val Lys Asp Gly Asp Leu Leu Val Gly 
        835                 840                 845             


Lys Val Thr Pro Lys Gly Met Thr Glu Leu Thr Ala Glu Glu Arg Leu 
    850                 855                 860                 


Leu His Ala Ile Phe Gly Glu Lys Ala Arg Glu Val Arg Asp Thr Ser 
865                 870                 875                 880 


Leu Arg Val Pro His Gly Gly Gly Ile Val Leu Asp Val Lys Val Phe 
                885                 890                 895     


Asn Arg Glu Asp Gly Asp Glu Leu Pro Pro Gly Val Asn Gln Leu Val 
            900                 905                 910         


Arg Val Tyr Ile Val Gln Lys Arg Lys Ile Ser Glu Gly Asp Lys Met 
        915                 920                 925             


Ala Gly Arg His Gly Asn Lys Gly Val Ile Ser Arg Ile Leu Pro Glu 
    930                 935                 940                 


Glu Asp Met Pro Phe Leu Pro Asp Gly Thr Pro Ile Asp Ile Met Leu 
945                 950                 955                 960 


Asn Pro Leu Gly Val Pro Ser Arg Met Asn Ile Gly Gln Val Phe Glu 
                965                 970                 975     


Leu His Leu Gly Met Ala Ala Lys Lys Leu Gly Leu His Ile Ala Ser 
            980                 985                 990         


Pro Val Phe Asp Gly Ala Thr Glu  Glu Asp Val Trp Asn  Ile Leu Glu 
        995                 1000                 1005             


Glu Ala  Gly Leu Ala Arg Asp  Ala Lys Thr Val Leu  Tyr Asp Gly 
    1010                 1015                 1020             


Arg Thr  Gly Glu Pro Phe Asp  Asn Arg Val Ser Val  Gly Ile Met 
    1025                 1030                 1035             


Tyr Met  Ile Lys Leu Ala His  Met Val Asp Asp Lys  Leu His Ala 
    1040                 1045                 1050             


Arg Ser  Thr Gly Pro Tyr Ser  Leu Val Thr Gln Gln  Pro Leu Gly 
    1055                 1060                 1065             


Gly Lys  Ala Gln Phe Gly Gly  Gln Arg Phe Gly Glu  Met Glu Val 
    1070                 1075                 1080             


Trp Ala  Leu Glu Ala Tyr Gly  Ala Ala Tyr Thr Leu  Gln Glu Ile 
    1085                 1090                 1095             


Leu Thr  Val Lys Ser Asp Asp  Val Val Gly Arg Val  Lys Thr Tyr 
    1100                 1105                 1110             


Glu Ala  Ile Val Lys Gly Glu  Asn Ile Pro Glu Pro  Gly Val Pro 
    1115                 1120                 1125             


Glu Ser  Phe Lys Val Leu Ile  Lys Glu Leu Gln Ser  Leu Gly Met 
    1130                 1135                 1140             


Asp Val  Thr Ile Leu Thr Ser  Asp Glu Gln Glu Val  Asn Met Glu 
    1145                 1150                 1155             


Asn Phe  Asp Asp Asp Asp Asp  His Ala Pro Asp Ala  Ile Met Val 
    1160                 1165                 1170             


Asp Val  Lys Pro Ala Glu Arg  Glu Glu Ala Gly Glu  Glu Lys Asp 
    1175                 1180                 1185             


Ala Val  Thr Lys Glu Gly Arg  Arg Ala Ala 
    1190                 1195             


<210>  3
<211>  1207
<212>  PRT
<213>  Geobacillus Stearothermphilus

<400>  3

Ser Gly Ser Ser Met Leu Asp Val Asn Lys Phe Glu Tyr Met Lys Ile 
1               5                   10                  15      


Gly Leu Ala Ser Pro Glu Lys Ile Arg Ser Trp Ser Tyr Gly Glu Val 
            20                  25                  30          


Lys Lys Pro Glu Thr Ile Asn Tyr Arg Thr Leu Lys Pro Glu Lys Asp 
        35                  40                  45              


Gly Leu Phe Glu Arg Ile Phe Gly Pro Thr Lys Asp Trp Glu Cys His 
    50                  55                  60                  


Cys Gly Lys Tyr Lys Arg Val Arg Tyr Lys Gly Val Val Cys Asp Arg 
65                  70                  75                  80  


Cys Gly Val Glu Val Thr Arg Ser Lys Val Arg Arg Glu Arg Met Gly 
                85                  90                  95      


His Ile Glu Leu Ala Ala Pro Val Ser His Ile Trp Tyr Phe Lys Gly 
            100                 105                 110         


Ile Pro Ser Arg Met Gly Leu Val Leu Asp Met Ser Pro Arg Ala Leu 
        115                 120                 125             


Glu Glu Val Ile Tyr Phe Ala Ser Tyr Val Val Thr Asp Pro Gly Asp 
    130                 135                 140                 


Thr Pro Leu Glu Lys Lys Gln Leu Leu Ser Glu Lys Glu Tyr Arg Ala 
145                 150                 155                 160 


Tyr Arg Glu Lys Tyr Gly Gln Ser Phe Gln Ala Ser Met Gly Ala Glu 
                165                 170                 175     


Ala Ile Lys Lys Leu Leu Gln Asp Ile Asp Leu Asp Lys Glu Val Ala 
            180                 185                 190         


Ala Leu Lys Glu Glu Leu Lys Thr Ala Gln Gly Gln Arg Arg Ala Arg 
        195                 200                 205             


Ile Ile Lys Arg Leu Glu Val Leu Glu Ala Phe Arg Ser Ser Gly Asn 
    210                 215                 220                 


Asp Pro Ala Trp Met Val Leu Asp Val Leu Pro Val Ile Pro Pro Glu 
225                 230                 235                 240 


Leu Arg Pro Met Val Gln Leu Asp Gly Gly Arg Phe Ala Thr Ser Asp 
                245                 250                 255     


Leu Asn Asp Leu Tyr Arg Arg Val Ile Asn Arg Asn Asn Arg Leu Lys 
            260                 265                 270         


Arg Leu Leu Asp Leu Gly Ala Pro Asn Ile Ile Val Gln Asn Glu Lys 
        275                 280                 285             


Arg Met Leu Gln Glu Ala Val Asp Ala Leu Ile Asp Asn Gly Arg Arg 
    290                 295                 300                 


Gly Arg Pro Val Thr Gly Pro Gly Asn Arg Pro Leu Lys Ser Leu Ser 
305                 310                 315                 320 


His Met Leu Lys Gly Lys Lys Gly Arg Phe Arg Gln Asn Leu Leu Gly 
                325                 330                 335     


Lys Arg Val Asp Tyr Ser Gly Arg Ser Val Ile Val Val Gly Pro Asn 
            340                 345                 350         


Leu Lys Met Tyr Gln Cys Gly Leu Pro Lys Glu Met Ala Leu Glu Leu 
        355                 360                 365             


Phe Lys Pro Phe Val Met Lys Glu Leu Val Glu Arg Gly Leu Ala His 
    370                 375                 380                 


Asn Ile Lys Ser Ala Lys Arg Lys Ile Glu Arg Val His Pro Glu Val 
385                 390                 395                 400 


Trp Asp Val Leu Glu Asp Val Ile Lys Glu His Pro Val Leu Leu Asn 
                405                 410                 415     


Arg Ala Pro Thr Leu His Arg Leu Gly Ile Gln Ala Phe Glu Pro Thr 
            420                 425                 430         


Leu Val Glu Gly Arg Ala Ile Arg Leu His Pro Leu Val Cys Thr Ala 
        435                 440                 445             


Tyr Asn Ala Asp Phe Asp Gly Asp Gln Met Ala Val His Val Pro Leu 
    450                 455                 460                 


Ser Ala Glu Ala Gln Ala Glu Ala Arg Leu Leu Met Leu Ala Ala Gln 
465                 470                 475                 480 


Asn Ile Leu Asn Pro Lys Asp Gly Lys Pro Val Val Thr Pro Ser Gln 
                485                 490                 495     


Asp Met Val Leu Gly Asn Tyr Tyr Leu Thr Met Glu Arg Glu Gly Ala 
            500                 505                 510         


Ile Gly Glu Gly Met Val Phe Lys Asp Thr Asp Glu Ala Leu Leu Ala 
        515                 520                 525             


Tyr His Asn Gly Tyr Val His Leu His Ser Arg Ile Ala Ile His Ala 
    530                 535                 540                 


Gly Ser Leu Lys Asn Glu Thr Phe Thr Glu Glu Gln Asn Asn Lys Leu 
545                 550                 555                 560 


Leu Leu Thr Thr Val Gly Lys Leu Ile Phe Asn Glu Ile Leu Pro Asn 
                565                 570                 575     


Ser Phe Pro Tyr Ile Asn Glu Pro Thr Thr Glu Asn Ile Glu Gly Arg 
            580                 585                 590         


Thr Pro Asp Lys Tyr Phe Leu Asp Lys Gly Val Asn Val Arg Glu Glu 
        595                 600                 605             


Ile Arg Lys Arg Glu Leu Val Pro Pro Phe Lys Lys Lys Val Leu Gly 
    610                 615                 620                 


Gln Ile Ile Ala Glu Val Phe Lys Arg Phe Lys Ile Thr Glu Thr Ser 
625                 630                 635                 640 


Lys Met Leu Asp Arg Met Lys Asp Leu Gly Phe Gln Tyr Ser Thr Lys 
                645                 650                 655     


Ala Gly Ile Thr Ile Gly Val Ala Asp Ile Val Val Leu Pro Glu Lys 
            660                 665                 670         


Gln Glu Ile Leu Asp Glu Ala Gln Ala Lys Val Asp Thr Val Leu Lys 
        675                 680                 685             


Gln Phe Arg Arg Gly Leu Ile Thr Asp Glu Glu Arg Tyr Glu Arg Val 
    690                 695                 700                 


Ile Ser Ile Trp Ser Ala Ala Lys Asp Lys Ile Gln Asp Arg Leu Met 
705                 710                 715                 720 


Lys Ser Leu Asp Lys Arg Asn Pro Ile Phe Met Met Ser Asp Ser Gly 
                725                 730                 735     


Ala Arg Gly Asn Ala Ser Asn Phe Thr Gln Leu Ala Gly Met Arg Gly 
            740                 745                 750         


Leu Met Ala Asn Pro Ala Gly Arg Ile Ile Glu Leu Pro Ile Lys Ser 
        755                 760                 765             


Ser Phe Arg Glu Gly Leu Thr Val Leu Glu Tyr Phe Ile Ser Thr His 
    770                 775                 780                 


Gly Ala Arg Lys Gly Leu Ala Asp Thr Ala Leu Lys Thr Ala Asp Ser 
785                 790                 795                 800 


Gly Tyr Leu Thr Arg Arg Leu Val Asp Val Ala Gln Asp Val Ile Val 
                805                 810                 815     


Arg Glu Glu Asp Cys Gly Thr Asp Arg Gly Ile Leu Ala Arg Ala Leu 
            820                 825                 830         


Thr Asp Gly Thr Glu Val Val Val Lys Leu Glu Glu Arg Leu Val Gly 
        835                 840                 845             


Arg Tyr Ala His Lys Thr Val Arg His Pro Glu Thr Gly Glu Val Ile 
    850                 855                 860                 


Val Arg Lys Asp Glu Met Ile Thr Glu Asp Ile Ala Asn Glu Ile Ile 
865                 870                 875                 880 


Lys Ala Gly Ile Thr Glu Val Trp Ile Arg Ser Val Phe Ala Cys Asn 
                885                 890                 895     


Thr Arg His Gly Val Cys Lys Lys Cys Tyr Gly Arg Asn Met Ala Thr 
            900                 905                 910         


Gly Met Asp Val Glu Val Gly Glu Ala Val Gly Ile Ile Ala Ala Gln 
        915                 920                 925             


Ser Ile Gly Glu Pro Gly Thr Gln Leu Thr Met Arg Thr Phe His Thr 
    930                 935                 940                 


Gly Gly Val Ala Gly Asp Asp Ile Thr Gln Gly Leu Pro Arg Val Gln 
945                 950                 955                 960 


Glu Leu Phe Glu Ala Arg Asn Pro Lys Gly Gln Ala Val Ile Ser Glu 
                965                 970                 975     


Ile Asp Gly Thr Val Ile Ser Ile Asn Glu Thr Arg Asp Asn Gln Tyr 
            980                 985                 990         


Glu Ile Val Val Gln Ser Glu Val  Glu Thr Arg Thr Tyr  Val Ala Pro 
        995                 1000                 1005             


Tyr Asn  Ala Arg Leu Lys Val  Glu Glu Gly Gln Arg  Val Glu Arg 
    1010                 1015                 1020             


Gly Gln  Glu Leu Thr Glu Gly  Ser Val Asp Pro Lys  Gln Leu Leu 
    1025                 1030                 1035             


Arg Val  Arg Asp Ile Thr Ser  Val Gln Glu Tyr Leu  Leu Arg Glu 
    1040                 1045                 1050             


Val Gln  Lys Val Tyr Arg Met  Gln Gly Val Glu Ile  Ser Asp Lys 
    1055                 1060                 1065             


His Ile  Glu Val Met Val Arg  Gln Met Leu Arg Lys  Val Arg Val 
    1070                 1075                 1080             


Ile Asp  Ala Gly Asp Thr Asp  Val Leu Pro Gly Thr  Leu Leu Asp 
    1085                 1090                 1095             


Val His  Gln Phe Thr Asp Val  Asn Ala Lys Ala Leu  Arg Glu Gly 
    1100                 1105                 1110             


Lys Arg  Pro Ala Thr Ala Arg  Gln Val Leu Leu Gly  Ile Thr Lys 
    1115                 1120                 1125             


Ala Ser  Leu Glu Thr Asp Ser  Phe Leu Ser Ala Ala  Ser Phe Gln 
    1130                 1135                 1140             


Glu Thr  Thr Arg Val Leu Thr  Asp Ala Ala Ile Lys  Gly Lys Arg 
    1145                 1150                 1155             


Asp Glu  Leu Leu Gly Leu Lys  Glu Asn Val Ile Ile  Gly Lys Leu 
    1160                 1165                 1170             


Val Pro  Ala Gly Thr Gly Met  Ala Arg Tyr Arg Lys  Val Lys Pro 
    1175                 1180                 1185             


Ala Val  Lys Lys Glu Thr Ala  Gly Gly Thr Val Ser  Ser Lys Gly 
    1190                 1195                 1200             


Arg Arg  Ala Ala 
    1205         


<210>  4
<211>  1207
<212>  PRT
<213>  Geobacillus Stearothermphilus

<400>  4

Ser Gly Ser Ser Met Leu Asp Val Asn Lys Phe Glu Tyr Met Lys Ile 
1               5                   10                  15      


Gly Leu Ala Ser Pro Glu Lys Ile Arg Ser Trp Ser Tyr Gly Glu Val 
            20                  25                  30          


Lys Lys Pro Glu Thr Ile Asn Tyr Arg Thr Leu Lys Pro Glu Lys Asp 
        35                  40                  45              


Gly Leu Phe Cys Glu Arg Ile Phe Gly Pro Thr Lys Asp Trp Glu Cys 
    50                  55                  60                  


His Cys Gly Lys Tyr Lys Arg Val Arg Tyr Lys Gly Val Val Cys Asp 
65                  70                  75                  80  


Arg Cys Gly Val Glu Val Thr Arg Ser Lys Val Arg Arg Glu Arg Met 
                85                  90                  95      


Gly His Ile Glu Leu Ala Ala Pro Val Ser His Ile Trp Tyr Phe Lys 
            100                 105                 110         


Gly Ile Pro Ser Arg Met Gly Leu Val Leu Asp Met Ser Pro Arg Ala 
        115                 120                 125             


Leu Glu Glu Val Ile Tyr Phe Ala Ser Tyr Val Val Thr Asp Pro Gly 
    130                 135                 140                 


Asp Thr Pro Leu Glu Lys Lys Gln Leu Leu Ser Glu Lys Glu Tyr Arg 
145                 150                 155                 160 


Ala Tyr Arg Glu Lys Tyr Gly Gln Ser Phe Gln Ala Ser Met Gly Ala 
                165                 170                 175     


Glu Ala Ile Lys Lys Leu Leu Gln Asp Ile Asp Leu Asp Lys Glu Val 
            180                 185                 190         


Ala Ala Leu Lys Glu Glu Leu Lys Thr Ala Gln Gly Gln Arg Arg Ala 
        195                 200                 205             


Arg Ile Ile Lys Arg Leu Glu Val Leu Glu Ala Phe Arg Ser Ser Gly 
    210                 215                 220                 


Asn Asp Pro Ala Trp Met Val Leu Asp Val Leu Pro Val Ile Pro Pro 
225                 230                 235                 240 


Glu Leu Arg Pro Met Val Gln Leu Asp Gly Gly Arg Phe Ala Thr Ser 
                245                 250                 255     


Asp Leu Asn Asp Leu Tyr Arg Arg Val Ile Asn Arg Asn Asn Arg Leu 
            260                 265                 270         


Lys Arg Leu Leu Asp Leu Gly Ala Pro Asn Ile Ile Val Gln Asn Glu 
        275                 280                 285             


Lys Arg Met Leu Gln Glu Ala Val Asp Ala Leu Ile Asp Asn Gly Arg 
    290                 295                 300                 


Arg Gly Arg Pro Val Thr Gly Pro Gly Asn Arg Pro Leu Lys Ser Leu 
305                 310                 315                 320 


Ser His Met Leu Lys Gly Lys Lys Gly Arg Phe Arg Gln Asn Leu Leu 
                325                 330                 335     


Gly Lys Arg Val Asp Tyr Ser Gly Arg Ser Val Ile Val Val Gly Pro 
            340                 345                 350         


Asn Leu Lys Met Tyr Gln Cys Gly Leu Pro Lys Glu Met Ala Leu Glu 
        355                 360                 365             


Leu Phe Lys Pro Phe Val Met Lys Glu Leu Val Glu Arg Gly Leu Ala 
    370                 375                 380                 


His Asn Ile Lys Ser Ala Lys Arg Lys Ile Glu Arg Val His Pro Glu 
385                 390                 395                 400 


Val Trp Asp Val Leu Glu Asp Val Ile Lys Glu His Pro Val Leu Asn 
                405                 410                 415     


Arg Ala Pro Thr Leu His Arg Leu Gly Ile Gln Ala Phe Glu Pro Thr 
            420                 425                 430         


Leu Val Glu Gly Arg Ala Ile Arg Leu His Pro Leu Val Cys Thr Ala 
        435                 440                 445             


Tyr Asn Ala Asp Phe Asp Gly Asp Gln Met Ala Val His Val Pro Leu 
    450                 455                 460                 


Ser Ala Glu Ala Gln Ala Glu Ala Arg Leu Leu Met Leu Ala Ala Gln 
465                 470                 475                 480 


Asn Ile Leu Asn Pro Lys Asp Gly Lys Pro Val Val Thr Pro Ser Gln 
                485                 490                 495     


Asp Met Val Leu Gly Asn Tyr Tyr Leu Thr Met Glu Arg Glu Gly Ala 
            500                 505                 510         


Ile Gly Glu Gly Met Val Phe Lys Asp Thr Asp Glu Ala Leu Leu Ala 
        515                 520                 525             


Tyr His Asn Gly Tyr Val His Leu His Ser Arg Ile Ala Ile His Ala 
    530                 535                 540                 


Gly Ser Leu Lys Asn Glu Thr Phe Thr Glu Glu Gln Asn Asn Lys Leu 
545                 550                 555                 560 


Leu Leu Thr Thr Val Gly Lys Leu Ile Phe Asn Glu Ile Leu Pro Asn 
                565                 570                 575     


Ser Phe Pro Tyr Ile Asn Glu Pro Thr Thr Glu Asn Ile Glu Gly Arg 
            580                 585                 590         


Thr Pro Asp Lys Tyr Phe Leu Asp Lys Gly Val Asn Val Arg Glu Glu 
        595                 600                 605             


Ile Arg Lys Arg Glu Leu Val Pro Pro Phe Lys Lys Lys Val Leu Gly 
    610                 615                 620                 


Gln Ile Ile Ala Glu Val Phe Lys Arg Phe Lys Ile Thr Glu Thr Ser 
625                 630                 635                 640 


Lys Met Leu Asp Arg Met Lys Asp Leu Gly Phe Gln Tyr Ser Thr Lys 
                645                 650                 655     


Ala Gly Ile Thr Ile Gly Val Ala Asp Ile Val Val Leu Pro Glu Lys 
            660                 665                 670         


Gln Glu Ile Leu Asp Glu Ala Gln Ala Lys Val Asp Thr Val Leu Lys 
        675                 680                 685             


Gln Phe Arg Arg Gly Leu Ile Thr Asp Glu Glu Arg Tyr Glu Arg Val 
    690                 695                 700                 


Ile Ser Ile Trp Ser Ala Ala Lys Asp Lys Ile Gln Asp Arg Leu Met 
705                 710                 715                 720 


Lys Ser Leu Asp Lys Arg Asn Pro Ile Phe Met Met Ser Asp Ser Gly 
                725                 730                 735     


Ala Arg Gly Asn Ala Ser Asn Phe Thr Gln Leu Ala Gly Met Arg Gly 
            740                 745                 750         


Leu Met Ala Asn Pro Ala Gly Arg Ile Ile Glu Leu Pro Ile Lys Ser 
        755                 760                 765             


Ser Phe Arg Glu Gly Leu Thr Val Leu Glu Tyr Phe Ile Ser Thr His 
    770                 775                 780                 


Gly Ala Arg Lys Gly Leu Ala Asp Thr Ala Leu Lys Thr Ala Asp Ser 
785                 790                 795                 800 


Gly Tyr Leu Thr Arg Arg Leu Val Asp Val Ala Gln Asp Val Ile Val 
                805                 810                 815     


Arg Glu Glu Asp Cys Gly Thr Asp Arg Gly Ile Leu Ala Arg Ala Leu 
            820                 825                 830         


Thr Asp Gly Thr Glu Val Val Val Lys Leu Glu Glu Arg Leu Val Gly 
        835                 840                 845             


Arg Tyr Ala His Lys Thr Val Arg His Pro Glu Thr Gly Glu Val Ile 
    850                 855                 860                 


Val Arg Lys Asp Glu Met Ile Thr Glu Asp Ile Ala Asn Glu Ile Ile 
865                 870                 875                 880 


Lys Ala Gly Ile Thr Glu Val Trp Ile Arg Ser Val Phe Ala Cys Asn 
                885                 890                 895     


Thr Arg His Gly Val Cys Lys Lys Cys Tyr Gly Arg Asn Met Ala Thr 
            900                 905                 910         


Gly Met Asp Val Glu Val Gly Glu Ala Val Gly Ile Ile Ala Ala Gln 
        915                 920                 925             


Ser Ile Gly Glu Pro Gly Thr Gln Leu Thr Met Arg Thr Phe His Thr 
    930                 935                 940                 


Gly Gly Val Ala Gly Asp Asp Ile Thr Gln Gly Leu Pro Arg Val Gln 
945                 950                 955                 960 


Glu Leu Phe Glu Ala Arg Asn Pro Lys Gly Gln Ala Val Ile Ser Glu 
                965                 970                 975     


Ile Asp Gly Thr Val Ile Ser Ile Asn Glu Thr Arg Asp Asn Gln Tyr 
            980                 985                 990         


Glu Ile Val Val Gln Ser Glu Val  Glu Thr Arg Thr Tyr  Val Ala Pro 
        995                 1000                 1005             


Tyr Asn  Ala Arg Leu Lys Val  Glu Glu Gly Gln Arg  Val Glu Arg 
    1010                 1015                 1020             


Gly Gln  Glu Leu Thr Glu Gly  Ser Val Asp Pro Lys  Gln Leu Leu 
    1025                 1030                 1035             


Arg Val  Arg Asp Ile Thr Ser  Val Gln Glu Tyr Leu  Leu Arg Glu 
    1040                 1045                 1050             


Val Gln  Lys Val Tyr Arg Met  Gln Gly Val Glu Ile  Ser Asp Lys 
    1055                 1060                 1065             


His Ile  Glu Val Met Val Arg  Gln Met Leu Arg Lys  Val Arg Val 
    1070                 1075                 1080             


Ile Asp  Ala Gly Asp Thr Asp  Val Leu Pro Gly Thr  Leu Leu Asp 
    1085                 1090                 1095             


Val His  Gln Phe Thr Asp Val  Asn Ala Lys Ala Leu  Arg Glu Gly 
    1100                 1105                 1110             


Lys Arg  Pro Ala Thr Ala Arg  Gln Val Leu Leu Gly  Ile Thr Lys 
    1115                 1120                 1125             


Ala Ser  Leu Glu Thr Asp Ser  Phe Leu Ser Ala Ala  Ser Phe Gln 
    1130                 1135                 1140             


Glu Thr  Thr Arg Val Leu Thr  Asp Ala Ala Ile Lys  Gly Lys Arg 
    1145                 1150                 1155             


Asp Glu  Leu Leu Gly Leu Lys  Glu Asn Val Ile Ile  Gly Lys Leu 
    1160                 1165                 1170             


Val Pro  Ala Gly Thr Gly Met  Ala Arg Tyr Arg Lys  Val Lys Pro 
    1175                 1180                 1185             


Ala Val  Lys Lys Glu Thr Ala  Gly Gly Thr Val Ser  Ser Lys Gly 
    1190                 1195                 1200             


Arg Arg  Ala Ala 
    1205         


<210>  5
<211>  193
<212>  PRT
<213>  Geobacillus Stearothermphilus

<400>  5

Ser Gly Ser Ser Met Ser Leu Gln Gln Gln Tyr Ser Pro Glu Glu Leu 
1               5                   10                  15      


Gln Glu Met Ser Phe Val Glu Leu Ala Asn Leu Ile Leu Leu Asp Lys 
            20                  25                  30          


Arg Glu Ala Leu Pro Phe Asp Gln Leu Val Arg Glu Ala Ala Ala Leu 
        35                  40                  45              


Ala Gly Ile Ser Glu Glu Glu Met Glu Ala Arg Leu Ala Gln Tyr Tyr 
    50                  55                  60                  


Thr Asp Leu Asn Ile Asp Gly Arg Phe Ile Cys Val Gly Glu Asn Val 
65                  70                  75                  80  


Trp Gly Arg Ala Trp Tyr Pro Phe Asp Gln Thr Glu Asp Glu Thr Val 
                85                  90                  95      


Thr Ile Val Lys Pro Lys Lys Lys Lys Lys Ala Leu Asp Asp Glu Tyr 
            100                 105                 110         


Asp Glu Tyr Asp Glu Leu Leu Glu Asp Glu Asp Leu Asp Tyr Asp Asp 
        115                 120                 125             


Leu Asp Glu Tyr Asp Glu Glu Glu Leu Glu Leu Asp Glu Asp Glu Leu 
    130                 135                 140                 


Leu Glu Asp Glu Glu Phe Asp Leu Asp Glu Asp Val Ala Phe Asp Asp 
145                 150                 155                 160 


Asp Ile Leu Gly Asp Glu Glu Phe Glu Leu Gly Asp Ala Pro Leu Asp 
                165                 170                 175     


Glu Glu Leu Asp Leu Glu Glu Pro Glu Glu Asp Glu Gly Arg Arg Ala 
            180                 185                 190         


Ala 
    


<210>  6
<211>  75
<212>  PRT
<213>  Geobacillus Stearothermphilus

<400>  6

Ser Gly Ser Ser Met Leu Tyr Pro Ser Ile Asp Leu Leu Met Gln Lys 
1               5                   10                  15      


Val Asp Ser Lys Tyr Lys Leu Val Thr Val Val Ala Lys Arg Ala Arg 
            20                  25                  30          


Glu Leu Gln Asp Gly Ala Glu Leu Met Val Lys Lys Pro Val Ser Lys 
        35                  40                  45              


Lys Phe Val Gly Gln Ala Leu Glu Glu Ile Ala Gly Asp Lys Val Glu 
    50                  55                  60                  


Leu Val Glu Glu Glu Lys Gly Arg Arg Ala Ala 
65                  70                  75  


<210>  7
<211>  408
<212>  PRT
<213>  Geobacillus Stearothermphilus

<400>  7

Met His His His His His His Gly Lys Pro Ile Pro Asn Pro Leu Leu 
1               5                   10                  15      


Gly Leu Asp Ser Thr Glu Asn Leu Tyr Phe Gln Gly Ile Asp Pro Phe 
            20                  25                  30          


Thr Met Ala Glu Lys Pro Ala Gln Ser Lys Gln Ala Glu Ala Ala Gly 
        35                  40                  45              


Glu Ser Leu Glu Gln Val Lys Glu Gln Leu Ala Glu Leu Gly Lys Lys 
    50                  55                  60                  


Arg Gly Ile Leu Thr Tyr Glu Glu Ile Ala Glu Arg Leu Ser Gly Phe 
65                  70                  75                  80  


Asp Leu Asp Ser Asp Gln Met Asp Glu Tyr Tyr Glu Tyr Leu Ala Glu 
                85                  90                  95      


Gln Gly Ile Glu Val Ile Ser Glu Ser Asp Leu Glu Ala Asp Pro Asp 
            100                 105                 110         


Ile Asp Asp Leu Ala Lys Glu Glu Glu Phe Asp Leu Asn Asp Leu Ser 
        115                 120                 125             


Val Pro Pro Gly Val Lys Ile Asn Asp Pro Val Arg Met Tyr Leu Lys 
    130                 135                 140                 


Glu Ile Gly Arg Val Pro Leu Leu Ser Ala Glu Glu Glu Ile Glu Leu 
145                 150                 155                 160 


Ala Lys Arg Ile Glu Gln Gly Asp Glu Glu Ala Lys Arg Arg Leu Thr 
                165                 170                 175     


Glu Ala Asn Leu Arg Leu Val Val Ser Ile Ala Lys Arg Tyr Val Gly 
            180                 185                 190         


Arg Gly Met Leu Phe Leu Asp Leu Ile Gln Glu Gly Asn Met Gly Leu 
        195                 200                 205             


Ile Lys Ala Val Glu Lys Phe Asp Tyr Arg Lys Gly Tyr Lys Phe Ser 
    210                 215                 220                 


Thr Tyr Ala Thr Trp Trp Ile Arg Gln Ala Ile Thr Arg Ala Ile Ala 
225                 230                 235                 240 


Asp Gln Ala Arg Thr Ile Arg Ile Pro Val His Met Val Glu Thr Ile 
                245                 250                 255     


Asn Lys Leu Ile Arg Val Gln Arg Gln Leu Leu Gln Asp Leu Gly Arg 
            260                 265                 270         


Glu Pro Thr Pro Glu Glu Ile Ala Glu Glu Met Asp Leu Thr Pro Glu 
        275                 280                 285             


Lys Val Arg Glu Ile Leu Lys Ile Ala Gln Glu Pro Val Ser Leu Glu 
    290                 295                 300                 


Thr Pro Ile Gly Glu Glu Asp Asp Ser His Leu Gly Asp Phe Ile Glu 
305                 310                 315                 320 


Asp Gln Asp Ala Thr Ser Pro Ser Glu His Ala Ala Tyr Glu Leu Leu 
                325                 330                 335     


Lys Glu Gln Leu Glu Asp Val Leu Asp Thr Leu Thr Asp Arg Glu Glu 
            340                 345                 350         


Asn Val Leu Arg Leu Arg Phe Gly Leu Asp Asp Gly Arg Thr Arg Thr 
        355                 360                 365             


Leu Glu Glu Val Gly Lys Val Phe Gly Val Thr Arg Glu Arg Ile Arg 
    370                 375                 380                 


Gln Ile Glu Ala Lys Ala Leu Arg Lys Leu Arg His Pro Ser Arg Ser 
385                 390                 395                 400 


Lys Arg Leu Lys Asp Phe Leu Glu 
                405             


<210>  8
<211>  224
<212>  PRT
<213>  Geobacillus Stearothermphilus

<400>  8

Ser Gly Ser Ser Met Asn Leu Val Leu Met Gly Leu Pro Gly Ala Gly 
1               5                   10                  15      


Lys Gly Thr Gln Ala Gly Lys Ile Val Glu Ala Tyr Gly Ile Pro His 
            20                  25                  30          


Ile Ser Thr Gly Asp Met Phe Arg Ala Ala Ile Lys Glu Gly Thr Pro 
        35                  40                  45              


Leu Gly Leu Gln Ala Lys Gln Tyr Met Asp Arg Gly Asp Leu Val Pro 
    50                  55                  60                  


Asp Glu Leu Gln Ala Lys Gln Tyr Met Asp Arg Gly Asp Leu Val Pro 
65                  70                  75                  80  


Asp Glu Gly Phe Leu Leu Asp Gly Phe Pro Arg Thr Val Ala Gln Ala 
                85                  90                  95      


Glu Ala Leu Glu Thr Leu Leu Ser Glu Ile Gly Arg Lys Leu Asp Tyr 
            100                 105                 110         


Val Ile His Ile Asp Val Arg Gln Glu Val Leu Met Glu Arg Leu Thr 
        115                 120                 125             


Gly Arg Arg Ile Cys Arg Asn Cys Gly Ala Thr Tyr His Leu Val Phe 
    130                 135                 140                 


His Pro Pro Ala Lys Pro Gly Val Cys Asp Lys Cys Gly Gly Asp Leu 
145                 150                 155                 160 


Tyr Gln Arg Pro Asp Asn Glu Ala Thr Val Ala Asn Arg Leu Glu Val 
                165                 170                 175     


Asn Met Lys Gln Met Lys Leu Leu Leu Asp Phe Tyr Glu Gln Lys Gly 
            180                 185                 190         


Tyr Leu Arg His Ile Asn Gly Glu Gln Glu Met Glu Lys Val Phe Ala 
        195                 200                 205             


Asp Ile Cys Glu Val Leu Gly Gly Leu Ala Arg Gly Arg Arg Ala Ala 
    210                 215                 220                 


<210>  9
<211>  697
<212>  PRT
<213>  Escherichia Coli

<400>  9

Ser Gly Ser Ser Met Gly Gln Glu Lys Leu Tyr Ile Glu Lys Glu Leu 
1               5                   10                  15      


Ser Trp Leu Ser Phe Asn Glu Arg Val Leu Gln Glu Ala Ala Asp Lys 
            20                  25                  30          


Ser Asn Pro Leu Ile Glu Arg Met Arg Phe Leu Gly Ile Tyr Ser Asn 
        35                  40                  45              


Asn Leu Asp Glu Phe Tyr Lys Val Arg Phe Ala Glu Leu Lys Arg Arg 
    50                  55                  60                  


Ile Ile Ile Ser Glu Glu Gln Gly Ser Asn Ser His Ser Arg His Leu 
65                  70                  75                  80  


Leu Gly Lys Ile Gln Ser Arg Val Leu Lys Ala Asp Gln Glu Phe Asp 
                85                  90                  95      


Gly Leu Tyr Asn Glu Leu Leu Leu Glu Met Ala Arg Asn Gln Ile Phe 
            100                 105                 110         


Leu Ile Asn Glu Arg Gln Leu Ser Val Asn Gln Gln Asn Trp Leu Arg 
        115                 120                 125             


His Tyr Phe Lys Gln Tyr Leu Arg Gln His Ile Thr Pro Ile Leu Ile 
    130                 135                 140                 


Asn Pro Asp Thr Asp Leu Val Gln Phe Leu Lys Asp Asp Tyr Thr Tyr 
145                 150                 155                 160 


Leu Ala Val Glu Ile Ile Arg Gly Asp Thr Ile Arg Tyr Ala Leu Leu 
                165                 170                 175     


Glu Ile Pro Ser Asp Lys Val Pro Arg Phe Val Asn Leu Pro Pro Glu 
            180                 185                 190         


Ala Pro Arg Arg Arg Lys Pro Met Ile Leu Leu Asp Asn Ile Leu Arg 
        195                 200                 205             


Tyr Cys Leu Asp Asp Ile Phe Lys Gly Phe Phe Asp Tyr Asp Ala Leu 
    210                 215                 220                 


Asn Ala Tyr Ser Met Lys Met Thr Arg Asp Ala Glu Tyr Asp Leu Val 
225                 230                 235                 240 


His Glu Met Glu Ala Ser Leu Met Glu Leu Met Ser Ser Ser Leu Lys 
                245                 250                 255     


Gln Arg Leu Thr Ala Glu Pro Val Arg Phe Val Tyr Gln Arg Asp Met 
            260                 265                 270         


Pro Asn Ala Leu Val Glu Val Leu Arg Glu Lys Leu Thr Ile Ser Arg 
        275                 280                 285             


Tyr Asp Ser Ile Val Pro Gly Gly Arg Tyr His Asn Phe Lys Asp Phe 
    290                 295                 300                 


Ile Asn Phe Pro Asn Val Gly Lys Ala Asn Leu Val Asn Lys Pro Leu 
305                 310                 315                 320 


Pro Arg Leu Arg His Ile Trp Phe Asp Lys Ala Gln Phe Arg Asn Gly 
                325                 330                 335     


Phe Asp Ala Ile Arg Glu Arg Asp Val Leu Leu Tyr Tyr Pro Tyr His 
            340                 345                 350         


Thr Phe Glu His Val Leu Glu Leu Leu Arg Gln Ala Ser Phe Asp Pro 
        355                 360                 365             


Ser Val Leu Ala Ile Lys Ile Asn Ile Tyr Arg Val Ala Lys Asp Ser 
    370                 375                 380                 


Arg Ile Ile Asp Ser Met Ile His Ala Ala His Asn Gly Lys Lys Val 
385                 390                 395                 400 


Thr Val Val Val Glu Leu Gln Ala Arg Phe Asp Glu Glu Ala Asn Ile 
                405                 410                 415     


His Trp Ala Lys Arg Leu Thr Glu Ala Gly Val His Val Ile Phe Ser 
            420                 425                 430         


Ala Pro Gly Leu Lys Ile His Ala Lys Leu Phe Leu Ile Ser Arg Lys 
        435                 440                 445             


Glu Asn Gly Glu Val Val Arg Tyr Ala His Ile Gly Thr Gly Asn Phe 
    450                 455                 460                 


Asn Glu Lys Thr Ala Arg Leu Tyr Thr Asp Tyr Ser Leu Leu Thr Ala 
465                 470                 475                 480 


Asp Ala Arg Ile Thr Asn Glu Val Arg Arg Val Phe Asn Phe Ile Glu 
                485                 490                 495     


Asn Pro Tyr Arg Pro Val Thr Phe Asp Tyr Leu Met Val Ser Pro Gln 
            500                 505                 510         


Asn Ser Arg Arg Leu Leu Tyr Glu Met Val Asp Arg Glu Ile Ala Asn 
        515                 520                 525             


Ala Gln Gln Gly Leu Pro Ser Gly Ile Thr Leu Lys Leu Asn Asn Leu 
    530                 535                 540                 


Val Asp Lys Gly Leu Val Asp Arg Leu Tyr Ala Ala Ser Ser Ser Gly 
545                 550                 555                 560 


Val Pro Val Asn Leu Leu Val Arg Gly Met Cys Ser Leu Ile Pro Asn 
                565                 570                 575     


Leu Glu Gly Ile Ser Asp Asn Ile Arg Ala Ile Ser Ile Val Asp Arg 
            580                 585                 590         


Tyr Leu Glu His Asp Arg Val Tyr Ile Phe Glu Asn Gly Gly Asp Lys 
        595                 600                 605             


Lys Val Tyr Leu Ser Ser Ala Asp Trp Met Thr Arg Asn Ile Asp Tyr 
    610                 615                 620                 


Arg Ile Glu Val Ala Thr Pro Leu Leu Asp Pro Arg Leu Lys Gln Arg 
625                 630                 635                 640 


Val Leu Asp Ile Ile Asp Ile Leu Phe Ser Asp Thr Val Lys Ala Arg 
                645                 650                 655     


Tyr Ile Asp Lys Glu Leu Ser Asn Arg Tyr Val Pro Arg Gly Asn Arg 
            660                 665                 670         


Arg Lys Val Arg Ala Gln Leu Ala Ile Tyr Asp Tyr Ile Lys Ser Leu 
        675                 680                 685             


Glu Gln Pro Glu Gly Arg Arg Ala Ala 
    690                 695         


<210>  10
<211>  608
<212>  PRT
<213>  Thermus aquaticus

<400>  10

Met Arg Leu Leu Pro Glu Glu Ser Trp Leu Gln Phe Asn Arg Arg Val 
1               5                   10                  15      


Leu Arg Gln Ser Glu Arg Pro Asp Phe Pro Leu Leu Glu Arg Met Arg 
            20                  25                  30          


Phe Leu Ala Ile Trp Asn Arg Asn Leu Asp Glu Phe Phe Ala Ala Arg 
        35                  40                  45              


Ile Ala Lys Pro Phe Leu Lys His Arg Gly Ser Ala His Leu Arg Leu 
    50                  55                  60                  


Leu Lys Glu Ala Glu Asp Gln Ala Arg Leu Ala Glu Gly Arg Tyr Arg 
65                  70                  75                  80  


Ala Leu Leu Ala Glu Ala Ala Pro His Leu Lys Ile Leu Glu Pro Glu 
                85                  90                  95      


Glu Leu Asp Asp Leu Asp Trp Leu Tyr Phe Arg Val Phe Leu Ala Glu 
            100                 105                 110         


Val Val Val Pro Lys Thr Asp Leu Ile Ala Trp Glu Ala Ala Lys Glu 
        115                 120                 125             


Ala Ser His Gly Ala Leu Tyr Phe Ala Ser Glu Asp His Leu Val Arg 
    130                 135                 140                 


Leu Pro Gln Asp Leu Pro Arg Leu Leu Lys Val Pro Gly Arg Glu Gly 
145                 150                 155                 160 


Thr Tyr Val Arg Leu Gly Ala Leu Met Arg Ala Arg Ser Asp Leu Phe 
                165                 170                 175     


Leu Pro Arg Glu Ser Pro Leu Tyr Glu Phe Arg Val Leu Arg Leu Leu 
            180                 185                 190         


Glu Ser Glu Arg Ala Arg Ala Asp Trp Asp Glu Leu Ala Gln Ala Leu 
        195                 200                 205             


Glu Gly Arg Gln Glu Gly Leu Ser Thr Leu Leu Val Ala Glu Arg Gly 
    210                 215                 220                 


Phe Pro Lys Gly Trp Leu Glu Gly Leu Gln Glu Ala Leu Gly Leu Leu 
225                 230                 235                 240 


Pro Glu Glu Val Ile Leu Leu Ser Pro Pro Leu Asn Leu Thr Leu Val 
                245                 250                 255     


Glu Thr Leu Val Ala Glu Gly Pro Ser Arg Trp Arg Phe Pro Pro Leu 
            260                 265                 270         


Glu Pro Lys Arg Pro Arg Ala Phe Met Lys Asn Pro Leu Lys Arg Leu 
        275                 280                 285             


Gln Glu Lys Asp Leu Val Leu Tyr His Pro Phe Glu Asp Tyr Ala Ala 
    290                 295                 300                 


Leu Glu Arg Phe Ala Glu Ala Ala Leu Ser Glu Glu Val Glu Glu Val 
305                 310                 315                 320 


Tyr Ala Thr Leu Tyr Arg Ile Gly Glu Ala Asn Pro Leu Ala Glu Ala 
                325                 330                 335     


Leu Ile Gln Ala Ala Arg Ala Gly Lys Arg Val His Val Leu Leu Glu 
            340                 345                 350         


Pro Arg Ala Arg Phe Asp Glu Leu Leu Asn Leu Ser Trp Tyr Leu Arg 
        355                 360                 365             


Phe Leu Arg Ala Gly Val Ala Val Leu Pro Leu Ser Glu Arg Lys Val 
    370                 375                 380                 


His Ala Lys Ala Leu Leu Leu Leu Thr Gln Lys Gly Gly Tyr Ala His 
385                 390                 395                 400 


Leu Gly Thr Gly Asn Tyr Asn Pro Gln Asn Gly Arg Gln Tyr Thr Asp 
                405                 410                 415     


Phe Ser Leu Phe Thr Ala Arg Lys Glu Val Val Met Glu Val Ala Glu 
            420                 425                 430         


Phe Phe Arg Ala Leu Gln Glu Gly Arg Thr Pro Arg Leu Asn Leu Leu 
        435                 440                 445             


Lys Thr Gly Glu Ala Ile Gln Glu Leu Leu Leu Glu His Ile Arg Ala 
    450                 455                 460                 


Glu Ser His Pro Lys Gly Arg Ile Leu Leu Lys Cys Asn His Leu Thr 
465                 470                 475                 480 


Asp Pro Ala Leu Leu Glu Ala Leu Ala Arg Ala Ala Asp Lys Gly Ala 
                485                 490                 495     


Arg Val Asp Leu Ile Val Arg Ser Thr Leu Thr Leu Leu His Pro Arg 
            500                 505                 510         


Phe Arg Ala Arg Ser Leu Val Gly Arg Phe Leu Glu His Ala Arg Val 
        515                 520                 525             


Ala Ala Phe Tyr Gln Gly Gly Arg Trp Ala Leu Tyr Leu Thr Ser Ala 
    530                 535                 540                 


Asp Leu Met Pro Arg Asn Phe Gln Asn Arg Phe Glu Leu Leu Phe Pro 
545                 550                 555                 560 


Val Leu Asp Lys Glu Gly Lys Ala Lys Val Leu Glu Val Leu Lys Arg 
                565                 570                 575     


Gln Leu Lys Asp Asp Arg Asn Ala Phe Leu Leu Ser Pro Lys Gly Glu 
            580                 585                 590         


Thr Pro Leu Trp Gly Gly Arg His Asp Ala Gln Arg Ile Leu Ala Trp 
        595                 600                 605             


<210>  11
<211>  641
<212>  PRT
<213>  Thermus aquaticus

<400>  11

Met His His His His His His Gly Lys Pro Ile Pro Asn Pro Leu Leu 
1               5                   10                  15      


Gly Leu Asp Ser Thr Glu Asn Leu Tyr Phe Gln Gly Ile Asp Pro Phe 
            20                  25                  30          


Thr Met Arg Leu Leu Pro Glu Glu Ser Trp Leu Gln Phe Asn Arg Arg 
        35                  40                  45              


Val Leu Arg Gln Ser Glu Arg Pro Asp Phe Pro Leu Leu Glu Arg Met 
    50                  55                  60                  


Arg Phe Leu Ala Ile Trp Asn Arg Asn Leu Asp Glu Phe Phe Ala Ala 
65                  70                  75                  80  


Arg Ile Ala Lys Pro Phe Leu Lys His Arg Gly Ser Glu Ala His Leu 
                85                  90                  95      


Arg Leu Leu Lys Glu Ala Glu Asp Gln Ala Arg Leu Ala Glu Gly Arg 
            100                 105                 110         


Tyr Arg Ala Leu Leu Ala Glu Ala Ala Pro His Leu Lys Ile Leu Glu 
        115                 120                 125             


Pro Glu Glu Leu Asp Asp Leu Asp Trp Leu Tyr Phe Arg Val Phe Leu 
    130                 135                 140                 


Ala Glu Val Val Val Pro Lys Thr Asp Leu Ile Ala Trp Glu Ala Ala 
145                 150                 155                 160 


Lys Glu Ala Ser His Gly Ala Leu Tyr Phe Ala Ser Glu Asp His Leu 
                165                 170                 175     


Val Arg Leu Pro Gln Asp Leu Pro Arg Leu Leu Lys Val Pro Gly Arg 
            180                 185                 190         


Glu Gly Thr Tyr Val Arg Leu Gly Ala Leu Met Arg Ala Arg Ser Asp 
        195                 200                 205             


Leu Phe Leu Pro Arg Glu Ser Pro Leu Tyr Glu Phe Arg Val Leu Arg 
    210                 215                 220                 


Leu Leu Glu Ser Glu Arg Ala Arg Ala Asp Trp Asp Glu Leu Ala Gln 
225                 230                 235                 240 


Ala Leu Glu Gly Arg Gln Glu Gly Leu Ser Thr Leu Leu Val Ala Glu 
                245                 250                 255     


Arg Gly Phe Pro Lys Gly Trp Leu Glu Gly Leu Gln Glu Ala Leu Gly 
            260                 265                 270         


Leu Leu Pro Glu Glu Val Ile Leu Leu Ser Pro Pro Leu Asn Leu Thr 
        275                 280                 285             


Leu Val Glu Thr Leu Val Ala Glu Gly Pro Ser Arg Trp Arg Phe Pro 
    290                 295                 300                 


Pro Leu Glu Pro Lys Arg Pro Arg Ala Phe Met Lys Asn Pro Leu Lys 
305                 310                 315                 320 


Arg Leu Gln Glu Lys Asp Leu Val Leu Tyr His Pro Phe Glu Asp Tyr 
                325                 330                 335     


Ala Ala Leu Glu Arg Phe Ala Glu Ala Ala Leu Ser Glu Glu Val Glu 
            340                 345                 350         


Glu Val Tyr Ala Thr Leu Tyr Arg Ile Gly Glu Ala Asn Pro Leu Ala 
        355                 360                 365             


Glu Ala Leu Ile Gln Ala Ala Arg Ala Gly Lys Arg Val His Val Leu 
    370                 375                 380                 


Leu Glu Pro Arg Ala Arg Phe Asp Glu Leu Leu Asn Leu Ser Trp Tyr 
385                 390                 395                 400 


Leu Arg Phe Leu Arg Ala Gly Val Ala Val Leu Pro Leu Ser Glu Arg 
                405                 410                 415     


Lys Val His Ala Lys Ala Leu Leu Leu Leu Thr Gln Lys Gly Gly Tyr 
            420                 425                 430         


Ala His Leu Gly Thr Gly Asn Tyr Asn Pro Gln Asn Gly Arg Gln Tyr 
        435                 440                 445             


Thr Asp Phe Ser Leu Phe Thr Ala Arg Lys Glu Val Val Met Glu Val 
    450                 455                 460                 


Ala Glu Phe Phe Arg Ala Leu Gln Glu Gly Arg Thr Pro Arg Leu Asn 
465                 470                 475                 480 


Leu Leu Lys Thr Gly Glu Ala Ile Gln Glu Leu Leu Leu Glu His Ile 
                485                 490                 495     


Arg Ala Glu Ser His Pro Lys Gly Arg Ile Leu Leu Lys Cys Asn His 
            500                 505                 510         


Leu Thr Asp Pro Ala Leu Leu Glu Ala Leu Ala Arg Ala Ala Asp Lys 
        515                 520                 525             


Gly Ala Arg Val Asp Leu Ile Val Arg Ser Thr Leu Thr Leu Leu His 
    530                 535                 540                 


Pro Arg Phe Arg Ala Arg Ser Leu Val Gly Arg Phe Leu Glu His Ala 
545                 550                 555                 560 


Arg Val Ala Ala Phe Tyr Gln Gly Gly Arg Trp Ala Leu Tyr Leu Thr 
                565                 570                 575     


Ser Ala Asp Leu Met Pro Arg Asn Phe Gln Asn Arg Phe Glu Leu Leu 
            580                 585                 590         


Phe Pro Val Leu Asp Lys Glu Gly Lys Ala Lys Val Leu Glu Val Leu 
        595                 600                 605             


Lys Arg Gln Leu Lys Asp Asp Arg Asn Ala Phe Leu Leu Ser Pro Lys 
    610                 615                 620                 


Gly Glu Thr Pro Leu Trp Gly Gly Arg His Asp Ala Gln Arg Ile Ala 
625                 630                 635                 640 


Trp 
    


<210>  12
<211>  457
<212>  PRT
<213>  Clostridium Tetani

<400>  12

Met Pro Ile Thr Ile Asn Asn Phe Arg Tyr Ser Asp Pro Val Asn Asn 
1               5                   10                  15      


Asp Thr Ile Ile Met Met Glu Pro Pro Tyr Cys Lys Gly Leu Asp Ile 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Val Pro Glu 
        35                  40                  45              


Arg Tyr Glu Phe Gly Thr Lys Pro Glu Asp Phe Asn Pro Pro Ser Ser 
    50                  55                  60                  


Leu Ile Glu Gly Ala Ser Glu Tyr Tyr Asp Pro Asn Tyr Leu Arg Thr 
65                  70                  75                  80  


Asp Ser Asp Lys Asp Arg Phe Leu Gln Thr Met Val Lys Leu Phe Asn 
                85                  90                  95      


Arg Ile Lys Asn Asn Val Ala Gly Glu Ala Leu Leu Asp Lys Ile Ile 
            100                 105                 110         


Asn Ala Ile Pro Tyr Leu Gly Asn Ser Tyr Ser Leu Leu Asp Lys Phe 
        115                 120                 125             


Asp Thr Asn Ser Asn Ser Val Ser Phe Asn Leu Leu Glu Gln Asp Pro 
    130                 135                 140                 


Ser Gly Ala Thr Thr Lys Ser Ala Met Leu Thr Asn Leu Ile Ile Phe 
145                 150                 155                 160 


Gly Pro Gly Pro Val Leu Asn Lys Asn Glu Val Arg Gly Ile Val Leu 
                165                 170                 175     


Arg Val Asp Asn Lys Asn Tyr Phe Pro Cys Arg Asp Gly Phe Gly Ser 
            180                 185                 190         


Ile Met Gln Met Ala Phe Cys Pro Glu Tyr Val Pro Thr Phe Asp Asn 
        195                 200                 205             


Val Ile Glu Asn Ile Thr Ser Leu Thr Ile Gly Lys Ser Lys Tyr Phe 
    210                 215                 220                 


Gln Asp Pro Ala Leu Leu Leu Met His Glu Leu Ile His Val Leu His 
225                 230                 235                 240 


Gly Leu Tyr Gly Met Gln Val Ser Ser His Glu Ile Ile Pro Ser Lys 
                245                 250                 255     


Gln Glu Ile Tyr Met Gln His Thr Tyr Pro Ile Ser Ala Glu Glu Leu 
            260                 265                 270         


Phe Thr Phe Gly Gly Gln Asp Ala Asn Leu Ile Ser Ile Asp Ile Lys 
        275                 280                 285             


Asn Asp Leu Tyr Glu Lys Thr Leu Asn Asp Tyr Lys Ala Ile Ala Asn 
    290                 295                 300                 


Lys Leu Ser Gln Val Thr Ser Cys Asn Asp Pro Asn Ile Asp Ile Asp 
305                 310                 315                 320 


Ser Tyr Lys Gln Ile Tyr Gln Gln Lys Tyr Gln Phe Asp Lys Asp Ser 
                325                 330                 335     


Asn Gly Gln Tyr Ile Val Asn Glu Asp Lys Phe Gln Ile Leu Tyr Asn 
            340                 345                 350         


Ser Ile Met Tyr Gly Phe Thr Glu Ile Glu Leu Gly Lys Lys Phe Asn 
        355                 360                 365             


Ile Lys Thr Arg Leu Ser Tyr Phe Ser Met Asn His Asp Pro Val Lys 
    370                 375                 380                 


Ile Pro Asn Leu Leu Asp Asp Thr Ile Tyr Asn Asp Thr Glu Gly Phe 
385                 390                 395                 400 


Asn Ile Glu Ser Lys Asp Leu Lys Ser Glu Tyr Lys Gly Gln Asn Met 
                405                 410                 415     


Arg Val Asn Thr Asn Ala Phe Arg Asn Val Asp Gly Ser Gly Leu Val 
            420                 425                 430         


Ser Lys Leu Ile Gly Leu Cys Lys Lys Ile Ile Pro Pro Thr Asn Ile 
        435                 440                 445             


Arg Glu Asn Leu Tyr Asn Arg Thr Ala 
    450                 455         


<210>  13
<211>  8
<212>  PRT
<213>  Clostridium Tetani

<400>  13

Met His Glu Leu Ile His Val Leu 
1               5               


<210>  14
<211>  861
<212>  PRT
<213>  Clostridium Tetani

<400>  14

Ser Leu Thr Asp Leu Gly Gly Glu Leu Cys Ile Lys Ile Lys Asn Glu 
1               5                   10                  15      


Asp Leu Thr Phe Ile Ala Glu Lys Asn Ser Phe Ser Glu Glu Pro Phe 
            20                  25                  30          


Gln Asp Glu Ile Val Ser Tyr Asn Thr Lys Asn Lys Pro Leu Asn Phe 
        35                  40                  45              


Asn Tyr Ser Leu Asp Lys Ile Ile Val Asp Tyr Asn Leu Gln Ser Lys 
    50                  55                  60                  


Ile Thr Leu Pro Asn Asp Arg Thr Thr Pro Val Thr Lys Gly Ile Pro 
65                  70                  75                  80  


Tyr Ala Pro Glu Tyr Lys Ser Asn Ala Ala Ser Thr Ile Glu Ile His 
                85                  90                  95      


Asn Ile Asp Asp Asn Thr Ile Tyr Gln Tyr Leu Tyr Ala Gln Lys Ser 
            100                 105                 110         


Pro Thr Thr Leu Gln Arg Ile Thr Met Thr Asn Ser Val Asp Asp Ala 
        115                 120                 125             


Leu Ile Asn Ser Thr Lys Ile Tyr Ser Tyr Phe Pro Ser Val Ile Ser 
    130                 135                 140                 


Lys Val Asn Gln Gly Ala Gln Gly Ile Leu Phe Leu Gln Trp Val Arg 
145                 150                 155                 160 


Asp Ile Ile Asp Asp Phe Thr Asn Glu Ser Ser Gln Lys Thr Thr Ile 
                165                 170                 175     


Asp Lys Ile Ser Asp Val Ser Thr Ile Val Pro Tyr Ile Gly Pro Ala 
            180                 185                 190         


Leu Asn Ile Val Lys Gln Gly Tyr Glu Gly Asn Phe Ile Gly Ala Leu 
        195                 200                 205             


Glu Thr Thr Gly Val Val Leu Leu Leu Glu Tyr Ile Pro Glu Ile Thr 
    210                 215                 220                 


Leu Pro Val Ile Ala Ala Leu Ser Ile Ala Glu Ser Ser Thr Gln Lys 
225                 230                 235                 240 


Glu Lys Ile Ile Lys Thr Ile Asp Asn Phe Leu Glu Lys Arg Tyr Glu 
                245                 250                 255     


Lys Trp Ile Glu Val Tyr Lys Leu Val Lys Ala Lys Trp Leu Gly Thr 
            260                 265                 270         


Val Asn Thr Gln Phe Gln Lys Arg Ser Tyr Gln Met Tyr Arg Ser Leu 
        275                 280                 285             


Glu Tyr Gln Val Asp Ala Ile Lys Lys Ile Ile Asp Tyr Glu Tyr Lys 
    290                 295                 300                 


Ile Tyr Ser Gly Pro Asp Lys Glu Gln Ile Ala Asp Glu Ile Asn Asn 
305                 310                 315                 320 


Leu Lys Asn Lys Leu Glu Glu Lys Ala Asn Lys Ala Met Ile Asn Ile 
                325                 330                 335     


Asn Ile Phe Met Arg Glu Ser Ser Arg Ser Phe Leu Val Asn Gln Met 
            340                 345                 350         


Ile Asn Glu Ala Lys Lys Gln Leu Leu Glu Phe Asp Thr Gln Ser Lys 
        355                 360                 365             


Asn Ile Leu Met Gln Tyr Ile Lys Ala Asn Ser Lys Phe Ile Gly Ile 
    370                 375                 380                 


Thr Glu Leu Lys Lys Leu Glu Ser Lys Ile Asn Lys Val Phe Ser Thr 
385                 390                 395                 400 


Pro Ile Pro Phe Ser Tyr Ser Lys Asn Leu Asp Cys Trp Val Asp Asn 
                405                 410                 415     


Glu Glu Asp Ile Asp Val Ile Leu Lys Lys Ser Thr Ile Leu Asn Leu 
            420                 425                 430         


Asp Ile Asn Asn Asp Ile Ile Ser Asp Ile Ser Gly Phe Asn Ser Ser 
        435                 440                 445             


Val Ile Thr Tyr Pro Asp Ala Gln Leu Val Pro Gly Ile Asn Gly Lys 
    450                 455                 460                 


Ala Ile His Leu Val Asn Asn Glu Ser Ser Glu Val Ile Val His Lys 
465                 470                 475                 480 


Ala Met Asp Ile Glu Tyr Asn Asp Met Phe Asn Asn Phe Thr Val Ser 
                485                 490                 495     


Phe Trp Leu Arg Val Pro Lys Val Ser Ala Ser His Leu Glu Gln Tyr 
            500                 505                 510         


Gly Thr Asn Glu Tyr Ser Ile Ile Ser Ser Met Lys Lys His Ser Leu 
        515                 520                 525             


Ser Ile Gly Ser Gly Trp Ser Val Ser Leu Lys Gly Asn Asn Leu Ile 
    530                 535                 540                 


Trp Thr Leu Lys Asp Ser Ala Gly Glu Val Arg Gln Ile Thr Phe Arg 
545                 550                 555                 560 


Asp Leu Pro Asp Lys Phe Asn Ala Tyr Leu Ala Asn Lys Trp Val Phe 
                565                 570                 575     


Ile Thr Ile Thr Asn Asp Arg Leu Ser Ser Ala Asn Leu Tyr Ile Asn 
            580                 585                 590         


Gly Val Leu Met Gly Ser Ala Glu Ile Thr Gly Leu Gly Ala Ile Arg 
        595                 600                 605             


Glu Asp Asn Asn Ile Thr Leu Lys Leu Asp Arg Cys Asn Asn Asn Asn 
    610                 615                 620                 


Gln Tyr Val Ser Ile Asp Lys Phe Arg Ile Phe Cys Lys Ala Leu Asn 
625                 630                 635                 640 


Pro Lys Glu Ile Glu Lys Leu Tyr Thr Ser Tyr Leu Ser Ile Thr Phe 
                645                 650                 655     


Leu Arg Asp Phe Trp Gly Asn Pro Leu Arg Tyr Asp Thr Glu Tyr Tyr 
            660                 665                 670         


Leu Ile Pro Val Ala Ser Ser Ser Lys Asp Val Gln Leu Lys Asn Ile 
        675                 680                 685             


Thr Asp Tyr Met Tyr Leu Thr Asn Ala Pro Ser Tyr Thr Asn Gly Lys 
    690                 695                 700                 


Leu Asn Ile Tyr Tyr Arg Arg Leu Tyr Asn Gly Leu Lys Phe Ile Ile 
705                 710                 715                 720 


Lys Arg Tyr Thr Pro Asn Asn Glu Ile Asp Ser Phe Val Lys Ser Gly 
                725                 730                 735     


Asp Phe Ile Lys Leu Tyr Val Ser Tyr Asn Asn Asn Glu His Ile Val 
            740                 745                 750         


Gly Tyr Pro Lys Asp Gly Asn Ala Phe Asn Asn Leu Asp Arg Ile Leu 
        755                 760                 765             


Arg Val Gly Tyr Asn Ala Pro Gly Ile Pro Leu Tyr Lys Lys Met Glu 
    770                 775                 780                 


Ala Val Lys Leu Arg Asp Leu Lys Thr Tyr Ser Val Gln Leu Lys Leu 
785                 790                 795                 800 


Tyr Asp Asp Lys Asn Ala Ser Leu Gly Leu Val Gly Thr His Asn Gly 
                805                 810                 815     


Gln Ile Gly Asn Asp Pro Asn Arg Asp Ile Leu Ile Ala Ser Asn Trp 
            820                 825                 830         


Tyr Phe Asn His Leu Lys Asp Lys Ile Leu Gly Cys Asp Trp Tyr Phe 
        835                 840                 845             


Val Pro Thr Asp Glu Gly Trp Thr Asn Asp Gly Ser Ala 
    850                 855                 860     


<210>  15
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TEV site located on page 41 of the specification

<400>  15

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  16
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCherry-His6 located on page 41 of the specification

<400>  16

Gly Ser Gly Ser Asn Gly Ser Ser Gly Ser Val Ser Met Ala Ile Ile 
1               5                   10                  15      


Lys Glu Phe Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly 
            20                  25                  30          


His Glu Phe Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly 
        35                  40                  45              


Thr Gln Thr Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe 
    50                  55                  60                  


Ala Trp Asp Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr 
65                  70                  75                  80  


Val Lys His Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro 
                85                  90                  95      


Glu Gly Phe Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val 
            100                 105                 110         


Val Thr Val Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr 
        115                 120                 125             


Lys Val Lys Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met 
    130                 135                 140                 


Gln Lys Lys Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro 
145                 150                 155                 160 


Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys 
                165                 170                 175     


Asp Gly Gly His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys 
            180                 185                 190         


Lys Pro Val Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp 
        195                 200                 205             


Ile Thr Ser His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg 
    210                 215                 220                 


Ala Glu Gly Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Arg Ser 
225                 230                 235                 240 


Gly Gly Gly His His His His His His Gly Asp Leu 
                245                 250         


<210>  17
<211>  1577
<212>  PRT
<213>  Clostridium Tetani

<400>  17

Met Pro Ile Thr Ile Asn Asn Phe Arg Tyr Ser Asp Pro Val Asn Asn 
1               5                   10                  15      


Asp Thr Ile Ile Met Met Glu Pro Pro Tyr Cys Lys Gly Leu Asp Ile 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Val Pro Glu 
        35                  40                  45              


Arg Tyr Glu Phe Gly Thr Lys Pro Glu Asp Phe Asn Pro Pro Ser Ser 
    50                  55                  60                  


Leu Ile Glu Gly Ala Ser Glu Tyr Tyr Asp Pro Asn Tyr Leu Arg Thr 
65                  70                  75                  80  


Asp Ser Asp Lys Asp Arg Phe Leu Gln Thr Met Val Lys Leu Phe Asn 
                85                  90                  95      


Arg Ile Lys Asn Asn Val Ala Gly Glu Ala Leu Leu Asp Lys Ile Ile 
            100                 105                 110         


Asn Ala Ile Pro Tyr Leu Gly Asn Ser Tyr Ser Leu Leu Asp Lys Phe 
        115                 120                 125             


Asp Thr Asn Ser Asn Ser Val Ser Phe Asn Leu Leu Glu Gln Asp Pro 
    130                 135                 140                 


Ser Gly Ala Thr Thr Lys Ser Ala Met Leu Thr Asn Leu Ile Ile Phe 
145                 150                 155                 160 


Gly Pro Gly Pro Val Leu Asn Lys Asn Glu Val Arg Gly Ile Val Leu 
                165                 170                 175     


Arg Val Asp Asn Lys Asn Tyr Phe Pro Cys Arg Asp Gly Phe Gly Ser 
            180                 185                 190         


Ile Met Gln Met Ala Phe Cys Pro Glu Tyr Val Pro Thr Phe Asp Asn 
        195                 200                 205             


Val Ile Glu Asn Ile Thr Ser Leu Thr Ile Gly Lys Ser Lys Tyr Phe 
    210                 215                 220                 


Gln Asp Pro Ala Leu Leu Leu Met His Glu Leu Ile His Val Leu His 
225                 230                 235                 240 


Gly Leu Tyr Gly Met Gln Val Ser Ser His Glu Ile Ile Pro Ser Lys 
                245                 250                 255     


Gln Glu Ile Tyr Met Gln His Thr Tyr Pro Ile Ser Ala Glu Glu Leu 
            260                 265                 270         


Phe Thr Phe Gly Gly Gln Asp Ala Asn Leu Ile Ser Ile Asp Ile Lys 
        275                 280                 285             


Asn Asp Leu Tyr Glu Lys Thr Leu Asn Asp Tyr Lys Ala Ile Ala Asn 
    290                 295                 300                 


Lys Leu Ser Gln Val Thr Ser Cys Asn Asp Pro Asn Ile Asp Ile Asp 
305                 310                 315                 320 


Ser Tyr Lys Gln Ile Tyr Gln Gln Lys Tyr Gln Phe Asp Lys Asp Ser 
                325                 330                 335     


Asn Gly Gln Tyr Ile Val Asn Glu Asp Lys Phe Gln Ile Leu Tyr Asn 
            340                 345                 350         


Ser Ile Met Tyr Gly Phe Thr Glu Ile Glu Leu Gly Lys Lys Phe Asn 
        355                 360                 365             


Ile Lys Thr Arg Leu Ser Tyr Phe Ser Met Asn His Asp Pro Val Lys 
    370                 375                 380                 


Ile Pro Asn Leu Leu Asp Asp Thr Ile Tyr Asn Asp Thr Glu Gly Phe 
385                 390                 395                 400 


Asn Ile Glu Ser Lys Asp Leu Lys Ser Glu Tyr Lys Gly Gln Asn Met 
                405                 410                 415     


Arg Val Asn Thr Asn Ala Phe Arg Asn Val Asp Gly Ser Gly Leu Val 
            420                 425                 430         


Ser Lys Leu Ile Gly Leu Cys Lys Lys Ile Ile Pro Pro Thr Asn Ile 
        435                 440                 445             


Arg Glu Asn Leu Tyr Asn Arg Thr Ala Ser Leu Thr Asp Leu Gly Gly 
    450                 455                 460                 


Glu Leu Cys Ile Lys Ile Lys Asn Glu Asp Leu Thr Phe Ile Ala Glu 
465                 470                 475                 480 


Lys Asn Ser Phe Ser Glu Glu Pro Phe Gln Asp Glu Ile Val Ser Tyr 
                485                 490                 495     


Asn Thr Lys Asn Lys Pro Leu Asn Phe Asn Tyr Ser Leu Asp Lys Ile 
            500                 505                 510         


Ile Val Asp Tyr Asn Leu Gln Ser Lys Ile Thr Leu Pro Asn Asp Arg 
        515                 520                 525             


Thr Thr Pro Val Thr Lys Gly Ile Pro Tyr Ala Pro Glu Tyr Lys Ser 
    530                 535                 540                 


Asn Ala Ala Ser Thr Ile Glu Ile His Asn Ile Asp Asp Asn Thr Ile 
545                 550                 555                 560 


Tyr Gln Tyr Leu Tyr Ala Gln Lys Ser Pro Thr Thr Leu Gln Arg Ile 
                565                 570                 575     


Thr Met Thr Asn Ser Val Asp Asp Ala Leu Ile Asn Ser Thr Lys Ile 
            580                 585                 590         


Tyr Ser Tyr Phe Pro Ser Val Ile Ser Lys Val Asn Gln Gly Ala Gln 
        595                 600                 605             


Gly Ile Leu Phe Leu Gln Trp Val Arg Asp Ile Ile Asp Asp Phe Thr 
    610                 615                 620                 


Asn Glu Ser Ser Gln Lys Thr Thr Ile Asp Lys Ile Ser Asp Val Ser 
625                 630                 635                 640 


Thr Ile Val Pro Tyr Ile Gly Pro Ala Leu Asn Ile Val Lys Gln Gly 
                645                 650                 655     


Tyr Glu Gly Asn Phe Ile Gly Ala Leu Glu Thr Thr Gly Val Val Leu 
            660                 665                 670         


Leu Leu Glu Tyr Ile Pro Glu Ile Thr Leu Pro Val Ile Ala Ala Leu 
        675                 680                 685             


Ser Ile Ala Glu Ser Ser Thr Gln Lys Glu Lys Ile Ile Lys Thr Ile 
    690                 695                 700                 


Asp Asn Phe Leu Glu Lys Arg Tyr Glu Lys Trp Ile Glu Val Tyr Lys 
705                 710                 715                 720 


Leu Val Lys Ala Lys Trp Leu Gly Thr Val Asn Thr Gln Phe Gln Lys 
                725                 730                 735     


Arg Ser Tyr Gln Met Tyr Arg Ser Leu Glu Tyr Gln Val Asp Ala Ile 
            740                 745                 750         


Lys Lys Ile Ile Asp Tyr Glu Tyr Lys Ile Tyr Ser Gly Pro Asp Lys 
        755                 760                 765             


Glu Gln Ile Ala Asp Glu Ile Asn Asn Leu Lys Asn Lys Leu Glu Glu 
    770                 775                 780                 


Lys Ala Asn Lys Ala Met Ile Asn Ile Asn Ile Phe Met Arg Glu Ser 
785                 790                 795                 800 


Ser Arg Ser Phe Leu Val Asn Gln Met Ile Asn Glu Ala Lys Lys Gln 
                805                 810                 815     


Leu Leu Glu Phe Asp Thr Gln Ser Lys Asn Ile Leu Met Gln Tyr Ile 
            820                 825                 830         


Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr Glu Leu Lys Lys Leu Glu 
        835                 840                 845             


Ser Lys Ile Asn Lys Val Phe Ser Thr Pro Ile Pro Phe Ser Tyr Ser 
    850                 855                 860                 


Lys Asn Leu Asp Cys Trp Val Asp Asn Glu Glu Asp Ile Asp Val Ile 
865                 870                 875                 880 


Leu Lys Lys Ser Thr Ile Leu Asn Leu Asp Ile Asn Asn Asp Ile Ile 
                885                 890                 895     


Ser Asp Ile Ser Gly Phe Asn Ser Ser Val Ile Thr Tyr Pro Asp Ala 
            900                 905                 910         


Gln Leu Val Pro Gly Ile Asn Gly Lys Ala Ile His Leu Val Asn Asn 
        915                 920                 925             


Glu Ser Ser Glu Val Ile Val His Lys Ala Met Asp Ile Glu Tyr Asn 
    930                 935                 940                 


Asp Met Phe Asn Asn Phe Thr Val Ser Phe Trp Leu Arg Val Pro Lys 
945                 950                 955                 960 


Val Ser Ala Ser His Leu Glu Gln Tyr Gly Thr Asn Glu Tyr Ser Ile 
                965                 970                 975     


Ile Ser Ser Met Lys Lys His Ser Leu Ser Ile Gly Ser Gly Trp Ser 
            980                 985                 990         


Val Ser Leu Lys Gly Asn Asn Leu  Ile Trp Thr Leu Lys  Asp Ser Ala 
        995                 1000                 1005             


Gly Glu  Val Arg Gln Ile Thr  Phe Arg Asp Leu Pro  Asp Lys Phe 
    1010                 1015                 1020             


Asn Ala  Tyr Leu Ala Asn Lys  Trp Val Phe Ile Thr  Ile Thr Asn 
    1025                 1030                 1035             


Asp Arg  Leu Ser Ser Ala Asn  Leu Tyr Ile Asn Gly  Val Leu Met 
    1040                 1045                 1050             


Gly Ser  Ala Glu Ile Thr Gly  Leu Gly Ala Ile Arg  Glu Asp Asn 
    1055                 1060                 1065             


Asn Ile  Thr Leu Lys Leu Asp  Arg Cys Asn Asn Asn  Asn Gln Tyr 
    1070                 1075                 1080             


Val Ser  Ile Asp Lys Phe Arg  Ile Phe Cys Lys Ala  Leu Asn Pro 
    1085                 1090                 1095             


Lys Glu  Ile Glu Lys Leu Tyr  Thr Ser Tyr Leu Ser  Ile Thr Phe 
    1100                 1105                 1110             


Leu Arg  Asp Phe Trp Gly Asn  Pro Leu Arg Tyr Asp  Thr Glu Tyr 
    1115                 1120                 1125             


Tyr Leu  Ile Pro Val Ala Ser  Ser Ser Lys Asp Val  Gln Leu Lys 
    1130                 1135                 1140             


Asn Ile  Thr Asp Tyr Met Tyr  Leu Thr Asn Ala Pro  Ser Tyr Thr 
    1145                 1150                 1155             


Asn Gly  Lys Leu Asn Ile Tyr  Tyr Arg Arg Leu Tyr  Asn Gly Leu 
    1160                 1165                 1170             


Lys Phe  Ile Ile Lys Arg Tyr  Thr Pro Asn Asn Glu  Ile Asp Ser 
    1175                 1180                 1185             


Phe Val  Lys Ser Gly Asp Phe  Ile Lys Leu Tyr Val  Ser Tyr Asn 
    1190                 1195                 1200             


Asn Asn  Glu His Ile Val Gly  Tyr Pro Lys Asp Gly  Asn Ala Phe 
    1205                 1210                 1215             


Asn Asn  Leu Asp Arg Ile Leu  Arg Val Gly Tyr Asn  Ala Pro Gly 
    1220                 1225                 1230             


Ile Pro  Leu Tyr Lys Lys Met  Glu Ala Val Lys Leu  Arg Asp Leu 
    1235                 1240                 1245             


Lys Thr  Tyr Ser Val Gln Leu  Lys Leu Tyr Asp Asp  Lys Asn Ala 
    1250                 1255                 1260             


Ser Leu  Gly Leu Val Gly Thr  His Asn Gly Gln Ile  Gly Asn Asp 
    1265                 1270                 1275             


Pro Asn  Arg Asp Ile Leu Ile  Ala Ser Asn Trp Tyr  Phe Asn His 
    1280                 1285                 1290             


Leu Lys  Asp Lys Ile Leu Gly  Cys Asp Trp Tyr Phe  Val Pro Thr 
    1295                 1300                 1305             


Asp Glu  Gly Trp Thr Asn Asp  Gly Ser Ala Glu Asn  Leu Tyr Phe 
    1310                 1315                 1320             


Gln Ser  Gly Ser Gly Ser Asn  Gly Ser Ser Gly Ser  Val Ser Met 
    1325                 1330                 1335             


Ala Ile  Ile Lys Glu Phe Met  Arg Phe Lys Val His  Met Glu Gly 
    1340                 1345                 1350             


Ser Val  Asn Gly His Glu Phe  Glu Ile Glu Gly Glu  Gly Glu Gly 
    1355                 1360                 1365             


Arg Pro  Tyr Glu Gly Thr Gln  Thr Ala Lys Leu Lys  Val Thr Lys 
    1370                 1375                 1380             


Gly Gly  Pro Leu Pro Phe Ala  Trp Asp Ile Leu Ser  Pro Gln Phe 
    1385                 1390                 1395             


Met Tyr  Gly Ser Lys Ala Tyr  Val Lys His Pro Ala  Asp Ile Pro 
    1400                 1405                 1410             


Asp Tyr  Leu Lys Leu Ser Phe  Pro Glu Gly Phe Lys  Trp Glu Arg 
    1415                 1420                 1425             


Val Met  Asn Phe Glu Asp Gly  Gly Val Val Thr Val  Thr Gln Asp 
    1430                 1435                 1440             


Ser Ser  Leu Gln Asp Gly Glu  Phe Ile Tyr Lys Val  Lys Leu Arg 
    1445                 1450                 1455             


Gly Thr  Asn Phe Pro Ser Asp  Gly Pro Val Met Gln  Lys Lys Thr 
    1460                 1465                 1470             


Met Gly  Trp Glu Ala Ser Ser  Glu Arg Met Tyr Pro  Glu Asp Gly 
    1475                 1480                 1485             


Ala Leu  Lys Gly Glu Ile Lys  Gln Arg Leu Lys Leu  Lys Asp Gly 
    1490                 1495                 1500             


Gly His  Tyr Asp Ala Glu Val  Lys Thr Thr Tyr Lys  Ala Lys Lys 
    1505                 1510                 1515             


Pro Val  Gln Leu Pro Gly Ala  Tyr Asn Val Asn Ile  Lys Leu Asp 
    1520                 1525                 1530             


Ile Thr  Ser His Asn Glu Asp  Tyr Thr Ile Val Glu  Gln Tyr Glu 
    1535                 1540                 1545             


Arg Ala  Glu Gly Arg His Ser  Thr Gly Gly Met Asp  Glu Leu Tyr 
    1550                 1555                 1560             


Arg Ser  Gly Gly Gly His His  His His His His Gly  Asp Leu 
    1565                 1570                 1575         


<210>  18
<211>  72
<212>  DNA
<213>  Geobacillus Stearothermphilus

<400>  18
attctgcaaa atcctattgt ttatcatacc tgatataatg aaaagatacg acactaggag       60

tgaatggcca tg                                                           72


<210>  19
<211>  43
<212>  DNA
<213>  Bacteriophage T7

<400>  19
aatacgactc actatagggg aattgtgagc ggataacaat tcc                         43


<210>  20
<211>  43
<212>  DNA
<213>  Bacteriophage T7

<400>  20
cctctagaaa taattttgtt taactttaag aaggagatat acc                         43


<210>  21
<211>  3947
<212>  DNA
<213>  Clostridium Tetani

<400>  21
atgccaataa ccataaataa ttttagatat agtgatcctg ttaataatga tacaattatt       60

atgatggagc caccatactg taagggtcta gatatctatt ataaggcttt caaaataaca      120

gatcgtattt ggatagtgcc ggaaaggtat gaatttggga caaaacctga agattttaac      180

ccaccatctt cattaataga aggtgcatct gagtattacg atccaaatta tttaaggact      240

gattctgata aagatagatt tttacaaacc atggtaaaac tgtttaacag aattaaaaac      300

aatgtagcag gtgaagcctt attagataag ataataaatg ccatacctta ccttggaaat      360

tcatattcct tactagacaa gtttgataca aactctaatt cagtatcttt taatttatca      420

gaacaagacc ccagtggagc aactacaaaa tcagcaatgc tgacaaattt aataatattt      480

ggacctgggc ctgttttaaa taaaaatgag gttagaggta ttgtattgag ggtagataat      540

aaaaattact tcccatgtag agatggtttt ggttcaataa tgcaaatggc attttgccca      600

gaatatatac ccacttttga taatgtaata gaaaatatta cgtcactcac tattggcaaa      660

agcaaatatt ttcaagatcc agcattacta ttaatgcacg aacttataca tgtactacat      720

ggtttatacg gaatgcaggt atcaagccat gaaattattc catccaaaca agaaatttat      780

atgcagcata catatccaat aagtgctgaa gaactattca cttttggcgg acaggatgct      840

aatcttataa gtattgatat aaaaaacgat ttatatgaaa aaactttaaa tgattataaa      900

gctatagcta acaaacttag tcaagtcact agctgcaatg atcccaacat tgatattgat      960

agctacaaac aaatatatca acaaaaatat caattcgata aagatagcaa tggacaatat     1020

attgtaaatg aggataaatt tcagatacta tataatagca taatgtatgg ttttacagag     1080

attgaattgg gaaaaaaatt taatataaaa actagacttt cttattttag tatgaatcat     1140

gaccctgtaa aaattccaaa tttattagat gatacaattt acaatgatac agaaggattt     1200

aatatagaaa gcaaagatct gaaatctgaa tataaaggtc aaaatatgag ggtaaataca     1260

aatgctttta gaaatgttga tggatcaggc ctagtttcaa aacttattgg cttatgtaaa     1320

aaaattatac caccaacaaa tataagagaa aatttatata atagaactgc atcattaaca     1380

gatttaggag gagaattatg tataaaaatt aaaaatgaag atttaacttt tatagctgaa     1440

aaaaatagct tttcagaaga accatttcaa gatgaaacag ttagttataa tacaaaaaat     1500

aaaccattaa attttaatta ttcgctagat aaaattattt tagattataa tctacaaagt     1560

aaaattacat tacctaatga taggacaacc ccagttacaa aaggaattcc atatgctcca     1620

aaatataaaa gtaatgctgc aagtacaata gaaatacata atattgatga caatacaata     1680

tatcaatatt tgtatgctca aaaatctcct acaactctac aaagaataac tatgactaat     1740

tctgtcgatg acgcattaat aaattccacc aaaatatatt catattttcc atctgtaatc     1800

agtaaagtta accaaggtgc acaaggaatt ttattcttac agtgggtgag agatataatt     1860

gatgatttac caatgaatct tcacaaaaaa ctactattga taaaatttca gatgtatcca     1920

ctattgttcc ttatatagga cccgcattaa acattgtaaa acaaggctat gagggaaact     1980

ttataggtgc tttagaaact accggagtgg ttttattatt ggaatatatt ccagaaatta     2040

ctttaccagt aattgcagct ttatctatag cagaaagtag cacacaaaaa gaaaagataa     2100

taaaaacaat agataacttt ttagaaaaaa gatatgaaaa atggattgaa gtatataaac     2160

taataaaagc aaaatggtta ggcacagtta atacgcaatt ccaaaaaaga agttatcaaa     2220

tgtatagatc tttagaatat caagtagatg caataaaaaa aataatagac tatgaatata     2280

aaatatattc aggacctgat aaggaacaaa ttgccgacga aattaataat ctgaaaaaca     2340

aacttgaaga aaaggctaat aaagcaatga taaacataaa tatatttatg agggaaagtt     2400

ctagatcatt tttagttaat caaatgatta acgaagctaa aaagcagtta ttagagtttg     2460

atactcaaag caaaaatatt ttaatgcagt atataaaagc aaattctaaa tttataggta     2520

taactgaact aaaaaaatta gaatcaaaaa taaacaaagt tttttcaaca ccaattccat     2580

tttcttattc taaaaatctg gattgttggg ttgataatga agaagatata gatgttatat     2640

taaaaaagag tacaatttta aatttagata ttaataatga tattatatca gatatatctg     2700

ggtttaattc atctgtaata acatatccag atgctcaatt ggtgcccgga ataaatggca     2760

aagcaataca tttagtaaac aatgaatctt ctgaagttat agtgcataaa gctatggata     2820

ttgaatataa tgatatgttt aataatttta ccgttagctt ttggttgagg gttcctaaag     2880

tatctgctag tcatttagaa caatatggca caaatgagta ttcaataatt agctctatga     2940

aaaaatatag tctatcaata ggatctggtt ggagtgtatc acttaaaggt aataacttaa     3000

tatggacttt aaaagattcc gcgggagaag ttagacaaat aacttttagt gatttatctg     3060

ataaatttaa tgcttattta gcaaataaat gggtttttat aactattact aatgatagat     3120

tatcttctgc taatttgtat ataaatggag tacttatgaa aaatgcagaa attaccggtt     3180

taggagctat tagagaggat aataatataa cattaaaact agatagatgt aataataata     3240

atcaatacgt ttctattgat aaatttagga tattttgcaa agcattaaat ccaaaagaga     3300

ttgaaaaatt atacacaagt tatttatcta taaccttttt aagagacttc tggggaaacc     3360

ctttacgata tgatacagaa tattatttaa taccagtagc ttctagttct aaagatgttc     3420

aattgaaaaa tataacagat tatatgtatt tgacaaatgc gccatcgtat actaacggaa     3480

aattgaatat atattataga aggttatata gtggactaaa atttattata aaaagatata     3540

cacctaataa tgaaatagat tcttttgtta aatcaggtga ttttattaaa ttatatgtat     3600

catataacaa taatgagcac attgtaggtt atccgaaaga tggaaatgcc tttaataatc     3660

ttgatagaat tctaagagta ggttataatg ccccaggtat ccctctttat aaaaaaatgg     3720

aagcagtaaa attgcgtgat ttaaaaacct attctgtaca acttaaatta tatgatgata     3780

aaaatgcatc tttaggacta gtaggtatcc gtaatggtca aataggcaac gatccaaata     3840

gggatatatt aattgcaagc aactggtact ttaatcattt aaaagataaa actttaacat     3900

gtgattggta ctttgtacct acagatgaag gatggacaaa tgattaa                   3947


<210>  22
<211>  801
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEVsite, mCherry, His6 located on page 41 of the specification

<400>  22
gaaaacctgt acttccaatc caatattggt agtgggagca acggcagcag cggatccgtg       60

agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac      120

atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc      180

tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc      240

tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc      300

gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg      360

aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctcca ggacggcgag      420

ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag      480

aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg      540

aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc      600

aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc      660

aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc      720

gagggccgcc actccaccgg cggcatggac gagctgtaca agtaagcggc cgcactcgag      780

caccaccacc accaccactg a                                                801


