﻿                         SEQUENCE LISTING

<110>  江苏众红生物工程创药研究院有限公司 常州京森生物医药研究所有限公司
 
<120>  一种重组粉尘螨1类变应原蛋白及其制备方法和应用

<130>  PCT009

<160>  10    

<170>  PatentIn version 3.3

<210>  1
<211>  912
<212>  DNA
<213>  Dermatophagoides farinae

<400>  1
agaccagcat ctatcaagac atttgaagag ttcaagaaag cttttaacaa gaactacgcc     60

actgttgaag aggaagaggt cgctagaaag aactttttgg aatctttgaa gtatgttgag    120

gcaaacaaag gtgctattaa tcacttgtct gatttgtccc ttgacgaatt caagaacaga    180

tacttgatga gtgctgaagc ctttgagcaa ttgaaaacac agttcgacct taacgccgaa    240

acctcagcat gtagaattaa ctcagttaat gtccctagtg agttggatct tagaagtttg    300

agaaccgtta ctcctattag aatgcaaggt ggatgtggtt cttgctgggc cttctccgga    360

gttgcagcta ccgaatctgc ttacttggcc tacagacaga cttcattgga tcttagtgaa    420

caagagttgg ttgactgtgc ttcccagcat ggttgccacg gagacactat tcctagaggt    480

attgaataca tccaacagaa cggagttgtc gaagagagat catacccata tgttgctaga    540

gagcaacagt gtagaagacc taactcacaa cactacggta ttagtaacta ctgccagatc    600

tatccacctg atgttaagca aattagagaa gcattgacac agacccatac tgcaattgct    660

gtcattatcg gaatcaagga tttgagagct ttccaacatt acgacggtag aacaattatc    720

caacacgata acggatacca gccaaattat catgctgtta acattgtcgg ttacggatcc    780

acccaaggtg ttgattattg gatcgtcaga aactcttggg atactacatg gggtgattct    840

ggttacggat atttccaagc tggaaacaat ttgatgatga ttgaacagta cccttatgtt    900

gtcatcatgt aa                                                        912


<210>  2
<211>  912
<212>  DNA
<213>  Dermatophagoides farinae

<400>  2
cgtccagctt caatcaaaac ttttgaagaa ttcaaaaaag ccttcaacaa aaactatgcc     60

accgttgaag aggaagaagt tgcccgtaaa aactttttgg aatcattgaa atatgttgaa    120

gctaacaaag gtgccatcaa ccatttgtcc gatttgtcat tggatgaatt caaaaaccgt    180

tatttgatga gtgctgaagc ttttgaacaa ctcaaaactc aattcgattt gaatgccgaa    240

acaagcgctt gccgtatcaa ttcggttaac gttccatcgg aattggattt acgatcactg    300

cgaactgtca ctccaatccg tatgcaagga ggctgtggtt catgttgggc tttctctggt    360

gtcgccgcaa ctgaatcagc ttatttggcc taccgtaaca cgtctttgga tctttctgaa    420

caggaactcg tcgattgcgc atctcaacac ggatgtcacg gcgatacaat accaagaggc    480

atcgaataca tccaacaaaa tggtgtcgtt gaagaaagaa gctatccata cgttgcacga    540

gaacaacaat gccgacgacc aaattcgcaa cattacggta tctcaaacta ctgccaaatt    600

tatccaccag atgtgaaaca aatccgtgaa gctttgactc aaacacacac agctattgcc    660

gtcattattg gcattaaaga tttgagagct tttcaacatt atgatggacg aacaatcatt    720

caacatgaca atggttatca accaaactat catgccgtca acattgtcgg ttacggaagt    780

acacaaggcg tcgattattg gatcgtacga aacagttggg atactacctg gggtgatagc    840

ggatacggat atttccaagc cggaaacaac ctcatgatga tcgaacaata tccatatgtt    900

gtaatcatgt ga                                                        912


<210>  3
<211>  303
<212>  PRT
<213>  Dermatophagoides farinae

<400>  3

Arg Pro Ala Ser Ile Lys Thr Phe Glu Glu Phe Lys Lys Ala Phe Asn 
1               5                   10                  15      


Lys Asn Tyr Ala Thr Val Glu Glu Glu Glu Val Ala Arg Lys Asn Phe 
            20                  25                  30          


Leu Glu Ser Leu Lys Tyr Val Glu Ala Asn Lys Gly Ala Ile Asn His 
        35                  40                  45              


Leu Ser Asp Leu Ser Leu Asp Glu Phe Lys Asn Arg Tyr Leu Met Ser 
    50                  55                  60                  


Ala Glu Ala Phe Glu Gln Leu Lys Thr Gln Phe Asp Leu Asn Ala Glu 
65                  70                  75                  80  


Thr Ser Ala Cys Arg Ile Asn Ser Val Asn Val Pro Ser Glu Leu Asp 
                85                  90                  95      


Leu Arg Ser Leu Arg Thr Val Thr Pro Ile Arg Met Gln Gly Gly Cys 
            100                 105                 110         


Gly Ser Cys Trp Ala Phe Ser Gly Val Ala Ala Thr Glu Ser Ala Tyr 
        115                 120                 125             


Leu Ala Tyr Arg Gln Thr Ser Leu Asp Leu Ser Glu Gln Glu Leu Val 
    130                 135                 140                 


Asp Cys Ala Ser Gln His Gly Cys His Gly Asp Thr Ile Pro Arg Gly 
145                 150                 155                 160 


Ile Glu Tyr Ile Gln Gln Asn Gly Val Val Glu Glu Arg Ser Tyr Pro 
                165                 170                 175     


Tyr Val Ala Arg Glu Gln Gln Cys Arg Arg Pro Asn Ser Gln His Tyr 
            180                 185                 190         


Gly Ile Ser Asn Tyr Cys Gln Ile Tyr Pro Pro Asp Val Lys Gln Ile 
        195                 200                 205             


Arg Glu Ala Leu Thr Gln Thr His Thr Ala Ile Ala Val Ile Ile Gly 
    210                 215                 220                 


Ile Lys Asp Leu Arg Ala Phe Gln His Tyr Asp Gly Arg Thr Ile Ile 
225                 230                 235                 240 


Gln His Asp Asn Gly Tyr Gln Pro Asn Tyr His Ala Val Asn Ile Val 
                245                 250                 255     


Gly Tyr Gly Ser Thr Gln Gly Val Asp Tyr Trp Ile Val Arg Asn Ser 
            260                 265                 270         


Trp Asp Thr Thr Trp Gly Asp Ser Gly Tyr Gly Tyr Phe Gln Ala Gly 
        275                 280                 285             


Asn Asn Leu Met Met Ile Glu Gln Tyr Pro Tyr Val Val Ile Met 
    290                 295                 300             


<210>  4
<211>  223
<212>  PRT
<213>  Dermatophagoides farinae

<400>  4

Thr Ser Ala Cys Arg Ile Asn Ser Val Asn Val Pro Ser Glu Leu Asp 
1               5                   10                  15      


Leu Arg Ser Leu Arg Thr Val Thr Pro Ile Arg Met Gln Gly Gly Cys 
            20                  25                  30          


Gly Ser Cys Trp Ala Phe Ser Gly Val Ala Ala Thr Glu Ser Ala Tyr 
        35                  40                  45              


Leu Ala Tyr Arg Gln Thr Ser Leu Asp Leu Ser Glu Gln Glu Leu Val 
    50                  55                  60                  


Asp Cys Ala Ser Gln His Gly Cys His Gly Asp Thr Ile Pro Arg Gly 
65                  70                  75                  80  


Ile Glu Tyr Ile Gln Gln Asn Gly Val Val Glu Glu Arg Ser Tyr Pro 
                85                  90                  95      


Tyr Val Ala Arg Glu Gln Gln Cys Arg Arg Pro Asn Ser Gln His Tyr 
            100                 105                 110         


Gly Ile Ser Asn Tyr Cys Gln Ile Tyr Pro Pro Asp Val Lys Gln Ile 
        115                 120                 125             


Arg Glu Ala Leu Thr Gln Thr His Thr Ala Ile Ala Val Ile Ile Gly 
    130                 135                 140                 


Ile Lys Asp Leu Arg Ala Phe Gln His Tyr Asp Gly Arg Thr Ile Ile 
145                 150                 155                 160 


Gln His Asp Asn Gly Tyr Gln Pro Asn Tyr His Ala Val Asn Ile Val 
                165                 170                 175     


Gly Tyr Gly Ser Thr Gln Gly Val Asp Tyr Trp Ile Val Arg Asn Ser 
            180                 185                 190         


Trp Asp Thr Thr Trp Gly Asp Ser Gly Tyr Gly Tyr Phe Gln Ala Gly 
        195                 200                 205             


Asn Asn Leu Met Met Ile Glu Gln Tyr Pro Tyr Val Val Ile Met 
    210                 215                 220             


<210>  5
<211>  18
<212>  DNA
<213>  人工引物

<400>  5
tgtaaaacga cggccagt                                                   18


<210>  6
<211>  17
<212>  DNA
<213>  人工引物

<400>  6
caggaaacag ctatgac                                                    17


<210>  7
<211>  25
<212>  DNA
<213>  人工引物

<400>  7
ggtattaacg gtttcggacg tattg                                           25


<210>  8
<211>  25
<212>  DNA
<213>  人工引物

<400>  8
gatgttgaca gggtctctct cttgg                                           25


<210>  9
<211>  21
<212>  DNA
<213>  人工引物

<400>  9
gactggttcc aattgacaag c                                               21


<210>  10
<211>  20
<212>  DNA
<213>  人工引物

<400>  10
ggcaaatggc attctgacat                                                 20


