﻿                         SEQUENCE LISTING

<110>  江苏众红生物工程创药研究院有限公司 常州京森生物医药研究所有限公司
 
<120>  一种重组户尘螨1类变应原蛋白及其制备方法和应用

<130>  PCT007

<160>  10    

<170>  PatentIn version 3.3

<210>  1
<211>  912
<212>  DNA
<213>  Dermatophagoides pteronyssinus

<400>  1
agaccatcct ccatcaagac tttcgaagag tacaagaagg ctttcaacaa gtcctacgct     60

actttcgagg acgaagaggc tgctagaaag aacttcttgg aatccgttaa gtacgttcag    120

tccaacggtg gtgctatcaa ccacttgtct gacttgtctt tggacgagtt caagaacaga    180

ttcttgatgt ccgctgaagc tttcgagcac ttgaaaacac agttcgactt gaacgctgaa    240

actaacgctt gttccatcaa cggtaacgct ccagctgaaa tcgacttgag acagatgaga    300

actgttactc caatcagaat gcagggtggt tgtggttctt gttgggcttt ttctggtgtt    360

gctgctactg aatccgctta cttggcttac agacaacagt ccttggactt ggctgaacaa    420

gagttggttg actgtgcttc ccaacatggt tgtcacggtg acactattcc aagaggtatc    480

gagtacatcc agcacaacgg tgttgttcaa gaatcctact acagatacgt tgctagagag    540

cagtcctgta gaagaccaaa cgctcaaaga ttcggtatct ccaactactg tcagatctac    600

ccacctaacg ctaacaagat cagagaggct ttggctcaaa ctcactccgc tatcgctgtt    660

atcatcggta tcaaggactt ggacgctttc agacactacg acggtagaac tatcatccag    720

agagacaacg gttaccagcc aaactaccac gctgttaata tcgttggtta ctctaacgct    780

caaggtgttg actactggat cgttagaaac tcctgggaca ctaactgggg tgataacggt    840

tacggttact tcgctgctaa cattgacttg atgatgattg aagagtaccc atacgttgtt    900

atcttgcatt aa                                                        912


<210>  2
<211>  909
<212>  DNA
<213>  Dermatophagoides pteronyssinus

<400>  2
cgtccatcat cgatcaaaac ttttgaagaa tacaaaaaag ccttcaacaa aagttatgct     60

accttcgaag atgaagaagc tgcccgtaaa aactttttgg aatcagtaaa atatgttcaa    120

tcaaacggag gtgccatcaa ccatttgtcc gatttgtcgt tggatgaatt caaaaaccga    180

ttcttgatga gtgcagaagc ttttgaacac ctcaaaactc aattcgattt gaatgctgaa    240

actaacgcct gcagtatcaa tggaaatgct ccagctgaaa tcgatttgcg acaaatgcga    300

actgtcactc ccattcgtat gcaaggaggc tgtggttcat gttgggcttt ctctggtgtt    360

gccgcaactg aatcagctta tttggcttac cgtaatcaat cattggatct tgctgaacaa    420

gaattagtcg attgtgcttc ccaacacggt tgtaatggtg ataccattcc acgtggtatt    480

gaatacatcc aacataatgg tgtcgtccaa gaaagctact atcgatacgt tgcacgagaa    540

caatcatgcc gacgaccaaa tgcacaacgt ttcggtatct caaactattg ccaaatttac    600

ccaccaaatg caaacaaaat tcgtgaagct ttggctcaaa cccacagcgc tattgccgtc    660

attattggca tcaaagattt agacgctttc cgtcattatg atggccgaac aatcattcaa    720

cgcgataatg gttaccaacc aaactatcac gctgtcaaca ttgttggtta cagtaacgca    780

cagggtgtcg attattggat cgtacgaaac agttgggata ccaattgggg tgataatggt    840

tacggttatt ttgctgccaa catcgatttg atgatgattg aagaatatcc atatgttgtc    900

attctctaa                                                            909


<210>  3
<211>  302
<212>  PRT
<213>  Dermatophagoides pteronyssinus

<400>  3

Arg Pro Ser Ser Ile Lys Thr Phe Glu Glu Tyr Lys Lys Ala Phe Asn 
1               5                   10                  15      


Lys Ser Tyr Ala Thr Phe Glu Asp Glu Glu Ala Ala Arg Lys Asn Phe 
            20                  25                  30          


Leu Glu Ser Val Lys Tyr Val Gln Ser Asn Gly Gly Ala Ile Asn His 
        35                  40                  45              


Leu Ser Asp Leu Ser Leu Asp Glu Phe Lys Asn Arg Phe Leu Met Ser 
    50                  55                  60                  


Ala Glu Ala Phe Glu His Leu Lys Thr Gln Phe Asp Leu Asn Ala Glu 
65                  70                  75                  80  


Thr Asn Ala Cys Ser Ile Asn Gly Asn Ala Pro Ala Glu Ile Asp Leu 
                85                  90                  95      


Arg Gln Met Arg Thr Val Thr Pro Ile Arg Met Gln Gly Gly Cys Gly 
            100                 105                 110         


Ser Cys Trp Ala Phe Ser Gly Val Ala Ala Thr Glu Ser Ala Tyr Leu 
        115                 120                 125             


Ala Tyr Arg Gln Gln Ser Leu Asp Leu Ala Glu Gln Glu Leu Val Asp 
    130                 135                 140                 


Cys Ala Ser Gln His Gly Cys Asn Gly Asp Thr Ile Pro Arg Gly Ile 
145                 150                 155                 160 


Glu Tyr Ile Gln His Asn Gly Val Val Gln Glu Ser Tyr Tyr Arg Tyr 
                165                 170                 175     


Val Ala Arg Glu Gln Ser Cys Arg Arg Pro Asn Ala Gln Arg Phe Gly 
            180                 185                 190         


Ile Ser Asn Tyr Cys Gln Ile Tyr Pro Pro Asn Ala Asn Lys Ile Arg 
        195                 200                 205             


Glu Ala Leu Ala Gln Thr His Ser Ala Ile Ala Val Ile Ile Gly Ile 
    210                 215                 220                 


Lys Asp Leu Asp Ala Phe Arg His Tyr Asp Gly Arg Thr Ile Ile Gln 
225                 230                 235                 240 


Arg Asp Asn Gly Tyr Gln Pro Asn Tyr His Ala Val Asn Ile Val Gly 
                245                 250                 255     


Tyr Ser Asn Ala Gln Gly Val Asp Tyr Trp Ile Val Arg Asn Ser Trp 
            260                 265                 270         


Asp Thr Asn Trp Gly Asp Asn Gly Tyr Gly Tyr Phe Ala Ala Asn Ile 
        275                 280                 285             


Asp Leu Met Met Ile Glu Glu Tyr Pro Tyr Val Val Ile Leu 
    290                 295                 300         


<210>  4
<211>  222
<212>  PRT
<213>  Dermatophagoides pteronyssinus

<400>  4

Thr Asn Ala Cys Ser Ile Asn Gly Asn Ala Pro Ala Glu Ile Asp Leu 
1               5                   10                  15      


Arg Gln Met Arg Thr Val Thr Pro Ile Arg Met Gln Gly Gly Cys Gly 
            20                  25                  30          


Ser Cys Trp Ala Phe Ser Gly Val Ala Ala Thr Glu Ser Ala Tyr Leu 
        35                  40                  45              


Ala Tyr Arg Gln Gln Ser Leu Asp Leu Ala Glu Gln Glu Leu Val Asp 
    50                  55                  60                  


Cys Ala Ser Gln His Gly Cys His Gly Asp Thr Ile Pro Arg Gly Ile 
65                  70                  75                  80  


Glu Tyr Ile Gln His Asn Gly Val Val Gln Glu Ser Tyr Tyr Arg Tyr 
                85                  90                  95      


Val Ala Arg Glu Gln Ser Cys Arg Arg Pro Asn Ala Gln Arg Phe Gly 
            100                 105                 110         


Ile Ser Asn Tyr Cys Gln Ile Tyr Pro Pro Asn Ala Asn Lys Ile Arg 
        115                 120                 125             


Glu Ala Leu Ala Gln Thr His Ser Ala Ile Ala Val Ile Ile Gly Ile 
    130                 135                 140                 


Lys Asp Leu Asp Ala Phe Arg His Tyr Asp Gly Arg Thr Ile Ile Gln 
145                 150                 155                 160 


Arg Asp Asn Gly Tyr Gln Pro Asn Tyr His Ala Val Asn Ile Val Gly 
                165                 170                 175     


Tyr Ser Asn Ala Gln Gly Val Asp Tyr Trp Ile Val Arg Asn Ser Trp 
            180                 185                 190         


Asp Thr Asn Trp Gly Asp Asn Gly Tyr Gly Tyr Phe Ala Ala Asn Ile 
        195                 200                 205             


Asp Leu Met Met Ile Glu Glu Tyr Pro Tyr Val Val Ile Leu 
    210                 215                 220         


<210>  5
<211>  18
<212>  DNA
<213>  人工引物

<400>  5
tgtaaaacga cggccagt                                                   18


<210>  6
<211>  17
<212>  DNA
<213>  人工引物

<400>  6
caggaaacag ctatgac                                                    17


<210>  7
<211>  25
<212>  DNA
<213>  人工引物

<400>  7
ggtattaacg gtttcggacg tattg                                           25


<210>  8
<211>  25
<212>  DNA
<213>  人工引物

<400>  8
gatgttgaca gggtctctct cttgg                                           25


<210>  9
<211>  21
<212>  DNA
<213>  人工引物

<400>  9
gactggttcc aattgacaag c                                               21


<210>  10
<211>  21
<212>  DNA
<213>  人工引物

<400>  10
gcaaatggca ttctgacatc c                                               21


