                         SEQUENCE LISTING

<110>  Lee, Chi-Yu Gregory
 
<120>  RP215 CHIMERIC ANTIGEN RECEPTOR CONSTRUCT AND METHODS OF MAKING AND USING SAME

<130>  V101 0004/JAM


<150>  US 62/441,382
<151>  2017-01-01 

<150>  US 62/480,207
<151>  2017-03-31


<160>  16 

<170>  PatentIn version 3.5

<210>  1
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  1

Met Asp Pro Lys Gly Ser Leu Ser Trp Arg Ile Leu Leu Phe Leu Ser 
1               5                   10                  15      


Leu Ala Phe Glu Leu Ser Tyr Gly Gln Val Gln Leu Val Gln Ser Gly 
            20                  25                  30          


Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala 
        35                  40                  45              


Ser Gly Tyr Thr Phe Thr Asp Tyr Trp Met His Trp Val Arg Gln Ala 
    50                  55                  60                  


Pro Gly Gln Gly Leu Glu Trp Met Gly Ala Ile Asp Thr Ser Asp Ser 
65                  70                  75                  80  


Tyr Thr Arg Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Met Thr Val 
                85                  90                  95      


Asp Glu Ser Thr Ser Thr Val Tyr Met Glu Leu Ser Ser Leu Arg Ser 
            100                 105                 110         


Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Ser Ile Tyr Asp Trp Gly 
        115                 120                 125             


Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
    130                 135                 140                 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
145                 150                 155                 160 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                165                 170                 175     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            180                 185                 190         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        195                 200                 205             


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
    210                 215                 220                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys 
225                 230                 235                 240 


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
                245                 250                 255     


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            260                 265                 270         


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        275                 280                 285             


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    290                 295                 300                 


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
305                 310                 315                 320 


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                325                 330                 335     


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            340                 345                 350         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        355                 360                 365             


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    370                 375                 380                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
385                 390                 395                 400 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                405                 410                 415     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            420                 425                 430         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        435                 440                 445             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    450                 455                 460                 


Pro Gly 
465     


<210>  2
<211>  1401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  2
atggacccca agggcagcct gagctggaga atcctgctgt tcctgagcct ggccttcgag       60

ctgagctacg gccaggtgca gcttgttcag agtggcgccg aagtgaagaa gccaggcgct      120

tccgtgaagg tgagctgcaa ggcatcaggc tacaccttca ctgattattg gatgcactgg      180

gtgagacagg cacccggtca ggggctcgaa tggatgggcg ccatcgatac tagcgattcc      240

tataccagat acgcacagaa gtttcaggga agagttacca tgactgtcga tgaatctaca      300

agcaccgtct acatggagct gagcagcctg cggtctgagg acaccgctgt ttactactgt      360

gcccgctcca tctatgattg gggtcaagga accttggtca cagtgagttc tgctagcacc      420

aagggcccca gcgtgttccc tctggccccc agcagcaaga gcaccagcgg cggaaccgcc      480

gccctgggct gcctggtgaa ggactacttc cccgagcccg tgaccgtgtc ctggaacagc      540

ggcgctctga ccagcggagt gcacaccttc cctgccgtgc tgcagagcag cggcctgtac      600

tccctgagca gcgtggtgac cgtgcccagc agcagcctgg gcacccagac ctacatctgc      660

aacgtgaacc acaagccctc caacaccaag gtggacaaga aggtggagcc taagagctgc      720

gacaagaccc acacctgccc tccctgcccc gcccccgagc tgctgggcgg acccagcgtg      780

ttcctgttcc ctcccaagcc caaggacacc ctgatgatca gccgcacccc cgaggtgacc      840

tgcgtggtgg tggacgtgag ccacgaggac cccgaggtga agttcaactg gtacgtggac      900

ggcgtggagg tgcacaacgc caagaccaag cctcgggagg agcagtacaa ctccacctac      960

cgcgtggtga gcgtgctgac cgtgctgcac caggactggc tgaacggcaa ggagtacaag     1020

tgcaaggtga gcaacaaggc cctgcccgct cccatcgaga agaccatcag caaggccaag     1080

ggccagcccc gggagcctca ggtgtacacc ctgcccccca gccgcgacga gctgaccaag     1140

aaccaggtga gcctgacctg cctggtgaag ggcttctacc cctccgacat cgccgtggag     1200

tgggagagca acggccagcc tgagaacaac tacaagacca cccctcccgt gctggacagc     1260

gacggcagct tcttcctgta cagcaagctg accgtggaca agtcccggtg gcagcagggc     1320

aacgtgttca gctgcagcgt gatgcacgag gccctgcaca accactacac ccagaagagc     1380

ctgagcctga gccccggata g                                               1401


<210>  3
<211>  240
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  3

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala 
            20                  25                  30          


Val Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser 
        35                  40                  45              


Leu Leu Asn Ser Ser Asn Gln Lys Ser Tyr Leu Ala Trp Tyr Gln Gln 
    50                  55                  60                  


Lys Pro Gly Gln Pro Pro Lys Leu Leu Val Tyr Phe Ala Ser Thr Arg 
65                  70                  75                  80  


Glu Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
                85                  90                  95      


Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr 
            100                 105                 110         


Phe Cys Gln Gln His Tyr Ser Thr Pro Ser Thr Phe Gly Gly Gly Thr 
        115                 120                 125             


Lys Leu Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe 
    130                 135                 140                 


Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys 
145                 150                 155                 160 


Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val 
                165                 170                 175     


Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln 
            180                 185                 190         


Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser 
        195                 200                 205             


Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His 
    210                 215                 220                 


Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 235                 240 


<210>  4
<211>  723
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  4
atggagaccg acaccctgct gctctgggtg ctgctgctct gggtgcccgg ctccaccgga       60

gatatcgtga tgacccagtc ccccgacagc ctggccgtct ctctgggtga gcgggcaacc      120

atcaactgta agtctagcca gtccctgttg aacagtagca atcaaaagag ctatcttgcc      180

tggtatcagc aaaagcctgg ccagccacca aaactgcttg tctatttcgc ttccactcgg      240

gaaagcggtg taccagaccg cttttctggc tcaggttccg gcacagactt taccttgacc      300

attagctccc ttcaggcaga ggacgtggca gtctactttt gccagcaaca ctactccact      360

ccatcaacct ttggaggtgg cacaaaactg gagattaagc ggaccgtggc cgcccccagc      420

gtgttcatct tccctcccag cgacgagcag ctgaagtctg gcaccgccag cgtggtgtgc      480

ctgctgaaca acttctaccc ccgcgaggcc aaggtgcagt ggaaggtgga caacgccctg      540

cagagcggca acagccagga gagcgtgacc gagcaggact ccaaggacag cacctacagc      600

ctgagcagca ccctgaccct gagcaaggcc gactacgaga agcacaaggt gtacgcctgc      660

gaggtgaccc accagggact gtctagcccc gtgaccaaga gcttcaaccg gggcgagtgc      720

taa                                                                    723


<210>  5
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  5

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ala Ile Asp Thr Ser Asp Ser Tyr Thr Arg Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Val Asp Glu Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Ile Tyr Asp Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
            100                 105                 110         


Ser 
    


<210>  6
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  6

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Ser Asn Gln Lys Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Val Tyr Phe Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Phe Cys Gln Gln 
                85                  90                  95      


His Tyr Ser Thr Pro Ser Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys 
    


<210>  7
<211>  683
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  7

Ala Ala Thr Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala 
1               5                   10                  15      


Leu Leu Leu His Ala Ala Arg Pro Gln Val Gln Leu Val Gln Ser Gly 
            20                  25                  30          


Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala 
        35                  40                  45              


Ser Gly Tyr Thr Phe Thr Asp Tyr Trp Met His Trp Val Arg Gln Ala 
    50                  55                  60                  


Pro Gly Gln Gly Leu Glu Trp Met Gly Ala Ile Asp Thr Ser Asp Ser 
65                  70                  75                  80  


Tyr Thr Arg Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Met Thr Val 
                85                  90                  95      


Asp Glu Ser Thr Ser Thr Val Tyr Met Glu Leu Ser Ser Leu Arg Ser 
            100                 105                 110         


Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Ser Ile Tyr Asp Trp Gly 
        115                 120                 125             


Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln Ser Pro 
145                 150                 155                 160 


Asp Ser Leu Ala Val Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys 
                165                 170                 175     


Ser Ser Gln Ser Leu Leu Asn Ser Ser Asn Gln Lys Ser Tyr Leu Ala 
            180                 185                 190         


Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Val Tyr Phe 
        195                 200                 205             


Ala Ser Thr Arg Glu Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly 
    210                 215                 220                 


Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp 
225                 230                 235                 240 


Val Ala Val Tyr Phe Cys Gln Gln His Tyr Ser Thr Pro Ser Thr Phe 
                245                 250                 255     


Gly Gly Gly Thr Lys Leu Glu Ile Lys Thr Thr Thr Pro Ala Pro Arg 
            260                 265                 270         


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
        275                 280                 285             


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
    290                 295                 300                 


Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr 
305                 310                 315                 320 


Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg 
                325                 330                 335     


Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro 
            340                 345                 350         


Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu 
        355                 360                 365             


Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala 
    370                 375                 380                 


Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
385                 390                 395                 400 


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
                405                 410                 415     


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
            420                 425                 430         


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
        435                 440                 445             


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
    450                 455                 460                 


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
465                 470                 475                 480 


His Met Gln Ala Leu Pro Pro Arg Glu Gly Arg Gly Ser Leu Leu Thr 
                485                 490                 495     


Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Phe His Val Ser Phe 
            500                 505                 510         


Arg Tyr Ile Phe Gly Leu Pro Pro Leu Ile Leu Val Leu Leu Pro Val 
        515                 520                 525             


Ala Ser Ser Asp Cys Asp Ile Glu Gly Lys Asp Gly Lys Gln Tyr Glu 
    530                 535                 540                 


Ser Val Leu Met Val Ser Ile Asp Gln Leu Leu Asp Ser Met Lys Glu 
545                 550                 555                 560 


Ile Gly Ser Asn Cys Leu Asn Asn Glu Phe Asn Phe Phe Lys Arg His 
                565                 570                 575     


Ile Cys Asp Ala Asn Lys Glu Gly Met Phe Leu Phe Arg Ala Ala Arg 
            580                 585                 590         


Lys Leu Arg Gln Phe Leu Lys Met Asn Ser Thr Gly Asp Phe Asp Leu 
        595                 600                 605             


His Leu Leu Lys Val Ser Glu Gly Thr Thr Ile Leu Leu Asn Cys Thr 
    610                 615                 620                 


Gly Gln Val Lys Gly Arg Lys Pro Ala Ala Leu Gly Glu Ala Gln Pro 
625                 630                 635                 640 


Thr Lys Ser Leu Glu Glu Asn Lys Ser Leu Lys Glu Gln Lys Lys Leu 
                645                 650                 655     


Asn Asp Leu Cys Phe Leu Lys Arg Leu Leu Gln Glu Ile Lys Thr Cys 
            660                 665                 670         


Trp Asn Lys Ile Leu Met Gly Thr Lys Glu His 
        675                 680             


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  8
ggcactgaca attccgtggt                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  9
agggacgtag cagaaggacg                                                   20


<210>  10
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  10
acgtcctttc catggctgct cgc                                               23


<210>  11
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  11
gctgtcatct cttgtgggct gt                                                22


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  12
actcatggga gctgctggtt c                                                 21


<210>  13
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  13
cctgtcatgc ccacacaaat ctctcc                                            26


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  14
tgacagccgc ctagcatttc                                                   20


<210>  15
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  15
gctcgatatc agcagttctt gaag                                              24


<210>  16
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  16
cacgtggccc gagagctgca tc                                                22