                         SEQUENCE LISTING

<110>  Lin, Lung-Jr
       Hsu, Yi-Hua
       Hsu, Ethan
 
<120>  COMPOSITION FOR EDITING A NUCLEIC ACID SEQUENCE AND METHOD USING 
       THE SAME

<130>  29649US

<150>  US 62/437,347
<151>  2016-12-21

<160>  41    

<170>  PatentIn version 3.5

<210>  1
<211>  9536
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pAB-mCherry

<400>  1
gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      900

gtttaaactt aagcttggta ccgagctcgc caccatgagt actgcactcg caacgctggc      960

tgggaagctg gctgaacgtg tcggcatgga ttctgtcgac ccacaggaac tgatcaccac     1020

tcttcgccag acggcattta aaggtgatgc cagcgatgcg cagttcatcg cattactgat     1080

cgttgccaac cagtacggcc ttaatccgtg gacgaaagaa atttacgcct ttcctgataa     1140

gcagaatggc atcgttccgg tggtgggcgt tgatggctgg tcccgcatca tcaatgaaaa     1200

ccagcagttt gatggcatgg actttgagca ggacaatgaa tcctgtacat gccggattta     1260

ccgcaaggac cgtaatcatc cgatctgcgt taccgaatgg atggatgaat gccgccgcga     1320

accattcaaa actcgcgaag gcagagaaat cacggggccg tggcagtcgc atcccaaacg     1380

gatgttacgt cataaagcca tgattcagtg tgcccgtctg gccttcggat ttgctggtat     1440

ctatgacaag gatgaagccg agcgcattgt cgaaaatact gcatacactg cagaacgtca     1500

gccggaacgc gacatcactc cggttaacga tgaaaccatg caggagatta acactctgct     1560

gatcgccctg gataaaacat gggatgacga cttattgccg ctctgttccc agatatttcg     1620

ccgcgacatt cgtgcatcgt cagaactgac acaggccgaa gcagtaaaag ctcttggatt     1680

cctgaaacag aaagccgcag agcagaaggt ggcagcatga tagtgagatc cgcggccgca     1740

tagataactg atccagtgtg ctggaattaa ttcgctgtct gcgagggcca gctgttgggg     1800

tgagtactcc ctctcaaaag cgggcatgac ttctgcgcta agattgtcag tttccaaaaa     1860

cgaggaggat ttgatattca cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat     1920

ctggtcagaa aagacaatct ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg     1980

gccatacact tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc     2040

caggtccaac tgcaggtcga gcatgcatct agggcggcca attccgcccc tctccccccc     2100

acccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt     2160

gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc     2220

ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag     2280

gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac     2340

aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc     2400

tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc     2460

acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca     2520

aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt     2580

gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg     2640

gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaaccca caaggagacg     2700

accttccgcc accatgacac cggacattat cctgcagcgt accgggatcg atgtgagagc     2760

tgtcgaacag ggggatgatg cgtggcacaa attacggctc ggcgtcatca ccgcttcaga     2820

agttcacaac gtgatagcaa aaccccgctc cggaaagaag tggcctgaca tgaaaatgtc     2880

ctacttccac accctgcttg ctgaggtttg caccggtgtg gctccggaag ttaacgctaa     2940

agcactggcc tggggaaaac agtacgagaa cgacgccaga accctgtttg aattcacttc     3000

cggcgtgaat gttactgaat ccccgatcat ctatcgcgac gaaagtatgc gtaccgcctg     3060

ctctcccgat ggtttatgca gtgacggcaa cggccttgaa ctgaaatgcc cgtttacctc     3120

ccgggatttc atgaagttcc ggctcggtgg tttcgaggcc ataaagtcag cttacatggc     3180

ccaggtgcag tacagcatgt gggtgacgcg aaaaaatgcc tggtactttg ccaactatga     3240

cccgcgtatg aagcgtgaag gcctgcatta tgtcgtgatt gagcgggatg aaaagtacat     3300

ggcgagtttt gacgagatcg tgccggagtt catcgaaaaa atggacgagg cactggctga     3360

aattggtttt gtatttgggg agcaatggcg atgatctaga gatccgcggc cgcatagata     3420

actgatccag tgtgctggaa ttaattcgct gtctgcgagg gccagctgtt ggggtgagta     3480

ctccctctca aaagcgggca tgacttctgc gctaagattg tcagtttcca aaaacgagga     3540

ggatttgata ttcacctggc ccgcggtgat gcctttgagg gtggccgcgt ccatctggtc     3600

agaaaagaca atctttttgt tgtcaagctt gaggtgtggc aggcttgaga tctggccata     3660

cacttgagtg acaatgacat ccactttgcc tttctctcca caggtgtcca ctcccaggtc     3720

caactgcagg tcgagcatgc atctagggcg gccaattccg cccctctccc ccccacccct     3780

ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt     3840

ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac     3900

ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc     3960

aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa     4020

cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg     4080

gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg     4140

tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc     4200

tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat     4260

gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg     4320

tggttttcct ttgaaaaaca cgatgataag cttgccacaa cccacaagga gacgaccttc     4380

cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg     4440

cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg     4500

cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc     4560

cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt     4620

gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg     4680

ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct     4740

gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg     4800

ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga     4860

ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta     4920

cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta     4980

caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca     5040

gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaatc     5100

tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc     5160

tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct     5220

ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg     5280

gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg     5340

ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta     5400

tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt     5460

gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct     5520

cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg     5580

atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag     5640

tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa     5700

tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga     5760

tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa     5820

atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc     5880

tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga     5940

aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca     6000

accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat     6060

tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc     6120

tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag     6180

ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt     6240

cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta     6300

ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg     6360

tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa     6420

ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct     6480

gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg     6540

caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca     6600

atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat     6660

cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac     6720

gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc     6780

gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa     6840

aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag     6900

gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc     6960

ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt     7020

cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca     7080

acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa     7140

tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct     7200

tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca     7260

caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca     7320

tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat     7380

ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag     7440

ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg     7500

cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa     7560

tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca     7620

ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg     7680

taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc     7740

agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc     7800

cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac     7860

tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc     7920

tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata     7980

gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc     8040

acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca     8100

acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag     8160

cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta     8220

gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg     8280

gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc     8340

agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg     8400

acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga     8460

tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg     8520

agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct     8580

gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg     8640

agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc     8700

cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa     8760

ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc     8820

cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt     8880

cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc     8940

ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt     9000

tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc     9060

catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt     9120

gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata     9180

gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga     9240

tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag     9300

catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa     9360

aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt     9420

attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga     9480

aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc         9536


<210>  2
<211>  5728
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pAF-INTRON

<400>  2
ctcgaggtaa gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc       60

gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag      120

atccattcga ttagtgaacg gatctcgacg gtatcgatac tagtattatg cccagtacat      180

gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat      240

ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt      300

tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga      360

ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg      420

gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca      480

tccacgctgt tttgacctcc atagaagatt ctagaaccat ggctagcgtg agcaagggcg      540

aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc      600

acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga      660

agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga      720

cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca      780

agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca      840

actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc      900

tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact      960

acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact     1020

tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga     1080

acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt     1140

ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga     1200

ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtgatccgtt caactagcag     1260

accgtttaaa caattcaagc ttttttcaat tctcgacctc gagacaaatg gcagtattca     1320

tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag     1380

acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa     1440

attttcgggt ttattacagg gacagcagag atccagtttg gccgcggctc gagggggttg     1500

gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt     1560

ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc     1620

agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg     1680

cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc     1740

gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga     1800

ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg     1860

gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg     1920

cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac     1980

cgaatcaccg acctctctcc ccagggggat ccaccggagc ttaccatgac cgagtacaag     2040

cccacggtgc gcctcgccac ccgcgacgac gtccccaggg cggtacgcac cctcgccgcc     2100

gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg     2160

gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg     2220

gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg     2280

gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg     2340

cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg     2400

gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc     2460

cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc     2520

cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc     2580

gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac     2640

ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cgttaagagc tcggtacctt     2700

taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg     2760

gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt     2820

ctctctggtt agaccagatc tgagcctggg agctctctcg ctaactaggg aacccactgc     2880

ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg     2940

actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta     3000

gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga     3060

gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa     3120

atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca     3180

atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct     3240

aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc     3300

agacgcctgg ggtaatgact ctctagcttg aggcatcaaa taaaacgaaa ggctcagtcg     3360

aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca     3420

aatccgccct ctagagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca     3480

tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc     3540

gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta     3600

gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt     3660

gcggccgctg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg     3720

ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt     3780

atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa     3840

gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc     3900

gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag     3960

gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt     4020

gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg     4080

aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg     4140

ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg     4200

taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac     4260

tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg     4320

gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt     4380

taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg     4440

tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc     4500

tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt     4560

ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt     4620

taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag     4680

tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt     4740

cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc     4800

gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc     4860

cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg     4920

ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac     4980

aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg     5040

atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc     5100

tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact     5160

gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc     5220

aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat     5280

acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc     5340

ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac     5400

tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa     5460

aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact     5520

catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg     5580

atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg     5640

aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag     5700

gcgtatcacg aggccctttc gtcttcac                                        5728


<210>  3
<211>  322
<212>  DNA
<213>  Homo sapiens


<220>
<221>  exon
<222>  (1)..(322)

<400>  3
agc aaa ccg ccc aga gta gaa gat gga ttg ggg cac gct gca gac gat         48
Ser Lys Pro Pro Arg Val Glu Asp Gly Leu Gly His Ala Ala Asp Asp           
1               5                   10                  15                

cct ggg ggg tgt gaa caa aca ctc cac cag cat tgg aaa gat ctg gct         96
Pro Gly Gly Cys Glu Gln Thr Leu His Gln His Trp Lys Asp Leu Ala           
            20                  25                  30                    

cac cgt cct ctt cat ttt tcg cat tat gat cct cgt tgt ggc tgc aaa        144
His Arg Pro Leu His Phe Ser His Tyr Asp Pro Arg Cys Gly Cys Lys           
        35                  40                  45                        

gga ggt gtg ggg aga tga gca ggc cga ctt tgt ctg caa cac cct gca        192
Gly Gly Val Gly Arg     Ala Gly Arg Leu Cys Leu Gln His Pro Ala           
    50                      55                  60                        

gcc agg ctg caa gaa cgt gtg cta cga tca cta ctt ccc cat ctc cca        240
Ala Arg Leu Gln Glu Arg Val Leu Arg Ser Leu Leu Pro His Leu Pro           
    65                  70                  75                            

cat ccg gct atg ggc cct gca gct gat ctt cgt gtc cac gcc agc gct        288
His Pro Ala Met Gly Pro Ala Ala Asp Leu Arg Val His Ala Ser Ala           
80                  85                  90                  95            

cct agt ggc cat gca cgt ggc cta ccg gag aca t                          322
Pro Ser Gly His Ala Arg Gly Leu Pro Glu Thr                               
                100                 105                                   


<210>  4
<211>  321
<212>  DNA
<213>  Homo sapiens


<220>
<221>  exon
<222>  (1)..(321)

<400>  4
agc aaa ccg ccc aga gta gaa gat gga ttg ggg cac gct gca gac gat         48
Ser Lys Pro Pro Arg Val Glu Asp Gly Leu Gly His Ala Ala Asp Asp           
1               5                   10                  15                

cct ggg ggt gtg aac aaa cac tcc acc agc att gga aag atc tgg ctc         96
Pro Gly Gly Val Asn Lys His Ser Thr Ser Ile Gly Lys Ile Trp Leu           
            20                  25                  30                    

acc gtc ctc ttc att ttt cgc att atg atc ctc gtt gtg gct gca aag        144
Thr Val Leu Phe Ile Phe Arg Ile Met Ile Leu Val Val Ala Ala Lys           
        35                  40                  45                        

gag gtg tgg gga gat gag cag gcc gac ttt gtc tgc aac acc ctg cag        192
Glu Val Trp Gly Asp Glu Gln Ala Asp Phe Val Cys Asn Thr Leu Gln           
    50                  55                  60                            

cca ggc tgc aag aac gtg tgc tac gat cac tac ttc ccc atc tcc cac        240
Pro Gly Cys Lys Asn Val Cys Tyr Asp His Tyr Phe Pro Ile Ser His           
65                  70                  75                  80            

atc cgg cta tgg gcc ctg cag ctg atc ttc gtg tcc acg cca gcg ctc        288
Ile Arg Leu Trp Ala Leu Gln Leu Ile Phe Val Ser Thr Pro Ala Leu           
                85                  90                  95                

cta gtg gcc atg cac gtg gcc tac cgg aga cat                            321
Leu Val Ala Met His Val Ala Tyr Arg Arg His                               
            100                 105                                       


<210>  5
<211>  322
<212>  DNA
<213>  Homo sapiens


<220>
<221>  exon
<222>  (1)..(322)

<400>  5
agc aaa ccg ccc aga gta gaa gat gga ttg ggg cac gct gca gac gat         48
Ser Lys Pro Pro Arg Val Glu Asp Gly Leu Gly His Ala Ala Asp Asp           
1               5                   10                  15                

cct ggg ggg tgt gaa caa aca ctc cac cag cat tgg aaa gat ctg gct         96
Pro Gly Gly Cys Glu Gln Thr Leu His Gln His Trp Lys Asp Leu Ala           
            20                  25                  30                    

cac cgt cct ctt cat ttt tcg cat tat gat cct cat tgt ggc tgc aaa        144
His Arg Pro Leu His Phe Ser His Tyr Asp Pro His Cys Gly Cys Lys           
        35                  40                  45                        

gga ggt gtg ggg aga tga gca ggc cga ctt tgt ctg caa cac cct gca        192
Gly Gly Val Gly Arg     Ala Gly Arg Leu Cys Leu Gln His Pro Ala           
    50                      55                  60                        

gcc agg ctg caa gaa cgt gtg cta cga tca cta ctt ccc cat ctc cca        240
Ala Arg Leu Gln Glu Arg Val Leu Arg Ser Leu Leu Pro His Leu Pro           
    65                  70                  75                            

cat ccg gct atg ggc cct gca gct gat ctt cgt gtc cac gcc agc gct        288
His Pro Ala Met Gly Pro Ala Ala Asp Leu Arg Val His Ala Ser Ala           
80                  85                  90                  95            

cct agt ggc cat gca cgt ggc cta ccg gag aca t                          322
Pro Ser Gly His Ala Arg Gly Leu Pro Glu Thr                               
                100                 105                                   


<210>  6
<211>  3815
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GJB2-EX-AF


<220>
<221>  prim_transcript
<222>  (1)..(3815)

<400>  6
ggggtgcggt taaaaggcgc cacggcggga gacaggtgtt gcggccccgc agcgcccgcg       60

cgctcctctc cccgactcgg agcccctcgg cggcgcccgg cccaggaccc gcctaggagc      120

gcaggagccc cagcgcagag accccaacgc cgagaccccc gccccggccc cgccgcgctt      180

cctcccgacg caggtaggta agttaggcag ggatattcac cattatcgtt tcagacccac      240

ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga      300

gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata ctagtattat      360

gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc      420

gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac      480

tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa      540

aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt      600

aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc      660

tggagacgcc atccacgctg ttttgacctc catagaagat tctagaacca tggctagcgt      720

gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga      780

cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa      840

gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt      900

gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca      960

cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa     1020

ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa     1080

ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct     1140

ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat     1200

caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca     1260

ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct     1320

gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct     1380

ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtgatccgt     1440

tcaactagca gaccgtttaa acaattcaag cttttttcaa ttctcgacct cgagacaaat     1500

ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga     1560

aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac     1620

aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggccgcggct     1680

cgagggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct     1740

gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca     1800

cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc     1860

ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac     1920

aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg     1980

cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga     2040

gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt     2100

tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct     2160

ccctcgttga ccgaatcacc gacctctctc cccaggggga tccaccggag cttaccatga     2220

ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gcggtacgca     2280

ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc     2340

acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg     2400

gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg     2460

tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc     2520

ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg     2580

cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg     2640

ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga     2700

cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg     2760

tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc     2820

cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcgttaagag     2880

ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa     2940

agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc     3000

ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctc gctaactagg     3060

gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg     3120

tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat     3180

ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg     3240

aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa     3300

tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc     3360

caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc     3420

atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt     3480

tttatttatg cagagcaaac cgcccagagt agaagatgga ttggggcacg ctgcagacga     3540

tcctgggggg tgtgaacaaa cactccacca gcattggaaa gatctggctc accgtcctct     3600

tcatttttcg cattatgatc ctcgttgtgg ctgcaaagga ggtgtgggga gatgagcagg     3660

ccgactttgt ctgcaacacc ctgcagccag gctgcaagaa cgtgtgctac gatcactact     3720

tccccatctc ccacatccgg ctatgggccc tgcagctgat cttcgtgtcc acgccagcgc     3780

tcctagtggc catgcacgtg gcctaccgga gacat                                3815


<210>  7
<211>  3814
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GJB2-EX35-AF


<220>
<221>  prim_transcript
<222>  (1)..(3814)

<400>  7
ggggtgcggt taaaaggcgc cacggcggga gacaggtgtt gcggccccgc agcgcccgcg       60

cgctcctctc cccgactcgg agcccctcgg cggcgcccgg cccaggaccc gcctaggagc      120

gcaggagccc cagcgcagag accccaacgc cgagaccccc gccccggccc cgccgcgctt      180

cctcccgacg caggtaggta agttaggcag ggatattcac cattatcgtt tcagacccac      240

ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga      300

gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata ctagtattat      360

gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc      420

gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac      480

tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa      540

aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt      600

aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc      660

tggagacgcc atccacgctg ttttgacctc catagaagat tctagaacca tggctagcgt      720

gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga      780

cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa      840

gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt      900

gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca      960

cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa     1020

ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa     1080

ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct     1140

ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat     1200

caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca     1260

ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct     1320

gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct     1380

ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtgatccgt     1440

tcaactagca gaccgtttaa acaattcaag cttttttcaa ttctcgacct cgagacaaat     1500

ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga     1560

aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac     1620

aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggccgcggct     1680

cgagggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct     1740

gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca     1800

cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc     1860

ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac     1920

aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg     1980

cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga     2040

gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt     2100

tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct     2160

ccctcgttga ccgaatcacc gacctctctc cccaggggga tccaccggag cttaccatga     2220

ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gcggtacgca     2280

ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc     2340

acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg     2400

gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg     2460

tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc     2520

ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg     2580

cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg     2640

ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga     2700

cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg     2760

tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc     2820

cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcgttaagag     2880

ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa     2940

agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc     3000

ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctc gctaactagg     3060

gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg     3120

tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat     3180

ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg     3240

aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa     3300

tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc     3360

caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc     3420

atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt     3480

tttatttatg cagagcaaac cgcccagagt agaagatgga ttggggcacg ctgcagacga     3540

tcctgggggt gtgaacaaac actccaccag cattggaaag atctggctca ccgtcctctt     3600

catttttcgc attatgatcc tcgttgtggc tgcaaaggag gtgtggggag atgagcaggc     3660

cgactttgtc tgcaacaccc tgcagccagg ctgcaagaac gtgtgctacg atcactactt     3720

ccccatctcc cacatccggc tatgggccct gcagctgatc ttcgtgtcca cgccagcgct     3780

cctagtggcc atgcacgtgg cctaccggag acat                                 3814


<210>  8
<211>  3815
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GJB2-EX109-AF


<220>
<221>  prim_transcript
<222>  (1)..(3815)

<400>  8
ggggtgcggt taaaaggcgc cacggcggga gacaggtgtt gcggccccgc agcgcccgcg       60

cgctcctctc cccgactcgg agcccctcgg cggcgcccgg cccaggaccc gcctaggagc      120

gcaggagccc cagcgcagag accccaacgc cgagaccccc gccccggccc cgccgcgctt      180

cctcccgacg caggtaggta agttaggcag ggatattcac cattatcgtt tcagacccac      240

ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga      300

gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata ctagtattat      360

gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc      420

gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac      480

tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa      540

aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt      600

aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc      660

tggagacgcc atccacgctg ttttgacctc catagaagat tctagaacca tggctagcgt      720

gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga      780

cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa      840

gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt      900

gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca      960

cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa     1020

ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa     1080

ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct     1140

ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat     1200

caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca     1260

ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct     1320

gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct     1380

ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtgatccgt     1440

tcaactagca gaccgtttaa acaattcaag cttttttcaa ttctcgacct cgagacaaat     1500

ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga     1560

aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac     1620

aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggccgcggct     1680

cgagggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct     1740

gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca     1800

cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc     1860

ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac     1920

aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg     1980

cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga     2040

gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt     2100

tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct     2160

ccctcgttga ccgaatcacc gacctctctc cccaggggga tccaccggag cttaccatga     2220

ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gcggtacgca     2280

ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc     2340

acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg     2400

gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg     2460

tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc     2520

ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg     2580

cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg     2640

ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga     2700

cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg     2760

tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc     2820

cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcgttaagag     2880

ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa     2940

agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc     3000

ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctc gctaactagg     3060

gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg     3120

tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat     3180

ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg     3240

aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa     3300

tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc     3360

caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc     3420

atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt     3480

tttatttatg cagagcaaac cgcccagagt agaagatgga ttggggcacg ctgcagacga     3540

tcctgggggg tgtgaacaaa cactccacca gcattggaaa gatctggctc accgtcctct     3600

tcatttttcg cattatgatc ctcattgtgg ctgcaaagga ggtgtgggga gatgagcagg     3660

ccgactttgt ctgcaacacc ctgcagccag gctgcaagaa cgtgtgctac gatcactact     3720

tccccatctc ccacatccgg ctatgggccc tgcagctgat cttcgtgtcc acgccagcgc     3780

tcctagtggc catgcacgtg gcctaccgga gacat                                3815


<210>  9
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CMVpro-r


<220>
<221>  primer_bind
<222>  (1)..(39)

<400>  9
gcagtactca tggtggcgag ctcggtacca agcttaagt                              39


<210>  10
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cher-f


<220>
<221>  primer_bind
<222>  (1)..(39)

<400>  10
agacgacctt ccgccaccat ggtgagcaag ggcgaggag                              39


<210>  11
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  bet-f


<220>
<221>  primer_bind
<222>  (1)..(42)

<400>  11
cttggtaccg agctcgccac catgagtact gcactcgcaa cg                          42


<210>  12
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  bet-r


<220>
<221>  primer_bind
<222>  (1)..(37)

<400>  12
ccgcggatct cactatcatg ctgccacctt ctgctct                                37


<210>  13
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IRES-I-f


<220>
<221>  primer_bind
<222>  (1)..(35)

<400>  13
aaggtggcag catgatagtg agatccgcgg ccgca                                  35


<210>  14
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IRES-I-r


<220>
<221>  primer_bind
<222>  (1)..(36)

<400>  14
cggtgtcatg gtggcggaag gtcgtctcct tgtggg                                 36


<210>  15
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exo-f


<220>
<221>  primer_bind
<222>  (1)..(40)

<400>  15
agacgacctt ccgccaccat gacaccggac attatcctgc                             40


<210>  16
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exo-r


<220>
<221>  primer_bind
<222>  (1)..(38)

<400>  16
gccgcggatc tctagatcat cgccattgct ccccaaat                               38


<210>  17
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IRES-II-f


<220>
<221>  primer_bind
<222>  (1)..(35)

<400>  17
gagcaatggc gatgatctag agatccgcgg ccgca                                  35


<210>  18
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IRES-II-r


<220>
<221>  primer_bind
<222>  (1)..(40)

<400>  18
ccttgctcac catggtggcg gaaggtcgtc tccttgtggg                             40


<210>  19
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EB-VEC-f


<220>
<221>  primer_bind
<222>  (1)..(22)

<400>  19
acgcctgggg taatgactct ct                                                22


<210>  20
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EB-VEC-r


<220>
<221>  primer_bind
<222>  (1)..(21)

<400>  20
ctcgaggtga agacgaaagg g                                                 21


<210>  21
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  L122-VEC-f


<220>
<221>  primer_bind
<222>  (1)..(47)

<400>  21
ccctttcgtc ttcacctcga ggtaagttag gcagggatat tcaccat                     47


<210>  22
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  L122-U-r


<220>
<221>  primer_bind
<222>  (1)..(25)

<400>  22
tccctagtta gcgagagagc tccca                                             25


<210>  23
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  L122-D-f


<220>
<221>  primer_bind
<222>  (1)..(25)

<400>  23
gggagctctc tcgctaacta gggaa                                             25


<210>  24
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  L122-VEC-r


<220>
<221>  primer_bind
<222>  (1)..(49)

<400>  24
agagagtcat taccccaggc gtctgcataa ataaaaaaaa ttagtcagc                   49


<210>  25
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GJB-EX1-f


<220>
<221>  primer_bind
<222>  (1)..(19)

<400>  25
ggggtgcggt taaaaggcg                                                    19


<210>  26
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GJB-EX1-FU-r


<220>
<221>  primer_bind
<222>  (1)..(40)

<400>  26
gtgaatatcc ctgcctaact tacctgcgtc gggaggaagc                             40


<210>  27
<211>  9177
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCRISPR/Cas9n(D10A)L

<400>  27
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg cccaatccat cttctactcg ttttagagct agaaatagca agttaaaata      300

aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag      360

ctagaaatag caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc aattctgcag      420

acaaatggct ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc      480

gcccaacgac ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg      540

tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat      600

gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca      660

gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat      720

taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc      780

acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg      840

gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg      900

gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag      960

gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgac     1020

gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac     1080

tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt     1140

agctgagcaa gaggtaaggg tttaagggat ggttggttgg tggggtatta atgtttaatt     1200

acctggagca cctgcctgaa atcacttttt ttcaggttgg accggtgcca ccatggacta     1260

taaggaccac gacggagact acaaggatca tgatattgat tacaaagacg atgacgataa     1320

gatggcccca aagaagaagc ggaaggtcgg tatccacgga gtcccagcag ccgacaagaa     1380

gtacagcatc ggcctggcca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga     1440

gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa     1500

gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct     1560

gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga     1620

gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc     1680

cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga     1740

cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga     1800

cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt     1860

ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct     1920

gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag     1980

cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa     2040

tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggcaacc tgattgccct     2100

gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact     2160

gcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga     2220

ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga     2280

catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag     2340

atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc     2400

tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga     2460

cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga     2520

cggcaccgag gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac     2580

cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg     2640

gcggcaggaa gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct     2700

gaccttccgc atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg     2760

gatgaccaga aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa     2820

gggcgcttcc gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa     2880

cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct     2940

gaccaaagtg aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca     3000

gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct     3060

gaaagaggac tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga     3120

agatcggttc aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa     3180

ggacttcctg gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac     3240

actgtttgag gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga     3300

cgacaaagtg atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg     3360

gaagctgatc aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa     3420

gtccgacggc ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt     3480

taaagaggac atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat     3540

tgccaatctg gccggcagcc ccgccattaa gaagggcatc ctgcagacag tgaaggtggt     3600

ggacgagctc gtgaaagtga tgggccggca caagcccgag aacatcgtga tcgaaatggc     3660

cagagagaac cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat     3720

cgaagagggc atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac     3780

ccagctgcag aacgagaagc tgtacctgta ctacctgcag aatgggcggg atatgtacgt     3840

ggaccaggaa ctggacatca accggctgtc cgactacgat gtggaccata tcgtgcctca     3900

gagctttctg aaggacgact ccatcgacaa caaggtgctg accagaagcg acaagaaccg     3960

gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg     4020

gcagctgctg aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga     4080

gagaggcggc ctgagcgaac tggataaggc cggcttcatc aagagacagc tggtggaaac     4140

ccggcagatc acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga     4200

cgagaatgac aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc     4260

cgatttccgg aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc     4320

ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct     4380

ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa     4440

gagcgagcag gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa     4500

ctttttcaag accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga     4560

gacaaacggc gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg     4620

gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg     4680

cttcagcaaa gagtctatcc tgcccaagag gaacagcgat aagctgatcg ccagaaagaa     4740

ggactgggac cctaagaagt acggcggctt cgacagcccc accgtggcct attctgtgct     4800

ggtggtggcc aaagtggaaa agggcaagtc caagaaactg aagagtgtga aagagctgct     4860

ggggatcacc atcatggaaa gaagcagctt cgagaagaat cccatcgact ttctggaagc     4920

caagggctac aaagaagtga aaaaggacct gatcatcaag ctgcctaagt actccctgtt     4980

cgagctggaa aacggccgga agagaatgct ggcctctgcc ggcgaactgc agaagggaaa     5040

cgaactggcc ctgccctcca aatatgtgaa cttcctgtac ctggccagcc actatgagaa     5100

gctgaagggc tcccccgagg ataatgagca gaaacagctg tttgtggaac agcacaagca     5160

ctacctggac gagatcatcg agcagatcag cgagttctcc aagagagtga tcctggccga     5220

cgctaatctg gacaaagtgc tgtccgccta caacaagcac cgggataagc ccatcagaga     5280

gcaggccgag aatatcatcc acctgtttac cctgaccaat ctgggagccc ctgccgcctt     5340

caagtacttt gacaccacca tcgaccggaa gaggtacacc agcaccaaag aggtgctgga     5400

cgccaccctg atccaccaga gcatcaccgg cctgtacgag acacggatcg acctgtctca     5460

gctgggaggc gacaaaaggc cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa     5520

ggaattcggc agtggagagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa     5580

tcctggccca atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc     5640

cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt     5700

cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt     5760

cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac     5820

cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga     5880

gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg     5940

gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa     6000

gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc     6060

cgccttcctg gagacctccg cgccccacaa cctccccttc tacgagcggc tcggcttcac     6120

cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc     6180

cggtgcctga gaattctaac tagagctcgc tgatcagcct cgactgtgcc ttctagttgc     6240

cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc     6300

actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct     6360

attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga gaatagcagg     6420

catgctgggg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc     6480

gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg     6540

gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt     6600

ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc     6660

cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac     6720

ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg     6780

ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt     6840

tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc     6900

cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct     6960

tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga     7020

ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga     7080

attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg     7140

atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg     7200

cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt     7260

gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc     7320

tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc     7380

ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc     7440

cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga     7500

gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt     7560

ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag     7620

tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag     7680

aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta     7740

ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg     7800

agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca     7860

gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag     7920

gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc     7980

gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg     8040

tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc     8100

ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg     8160

cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt ggaagccgcg     8220

gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga     8280

cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac     8340

tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa     8400

aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca     8460

aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag     8520

gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac     8580

cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa     8640

ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc     8700

accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag     8760

tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac     8820

cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc     8880

gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc     8940

ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca     9000

cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc     9060

tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg     9120

ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt        9177


<210>  28
<211>  8508
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCRISPR/Cas9n(D10A)R

<400>  28
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg acaaacactc caccagcatg ttttagagct agaaatagca agttaaaata      300

aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag      360

ctagaaatag caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc aattctgcag      420

acaaatggct ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc      480

gcccaacgac ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg      540

tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat      600

gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca      660

gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat      720

taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc      780

acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg      840

gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg      900

gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag      960

gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgac     1020

gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac     1080

tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt     1140

agctgagcaa gaggtaaggg tttaagggat ggttggttgg tggggtatta atgtttaatt     1200

acctggagca cctgcctgaa atcacttttt ttcaggttgg accggtgcca ccatggacta     1260

taaggaccac gacggagact acaaggatca tgatattgat tacaaagacg atgacgataa     1320

gatggcccca aagaagaagc ggaaggtcgg tatccacgga gtcccagcag ccgacaagaa     1380

gtacagcatc ggcctggcca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga     1440

gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa     1500

gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct     1560

gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga     1620

gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc     1680

cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga     1740

cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga     1800

cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt     1860

ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct     1920

gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag     1980

cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa     2040

tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggcaacc tgattgccct     2100

gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact     2160

gcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga     2220

ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga     2280

catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag     2340

atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc     2400

tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga     2460

cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga     2520

cggcaccgag gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac     2580

cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg     2640

gcggcaggaa gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct     2700

gaccttccgc atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg     2760

gatgaccaga aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa     2820

gggcgcttcc gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa     2880

cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct     2940

gaccaaagtg aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca     3000

gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct     3060

gaaagaggac tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga     3120

agatcggttc aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa     3180

ggacttcctg gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac     3240

actgtttgag gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga     3300

cgacaaagtg atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg     3360

gaagctgatc aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa     3420

gtccgacggc ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt     3480

taaagaggac atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat     3540

tgccaatctg gccggcagcc ccgccattaa gaagggcatc ctgcagacag tgaaggtggt     3600

ggacgagctc gtgaaagtga tgggccggca caagcccgag aacatcgtga tcgaaatggc     3660

cagagagaac cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat     3720

cgaagagggc atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac     3780

ccagctgcag aacgagaagc tgtacctgta ctacctgcag aatgggcggg atatgtacgt     3840

ggaccaggaa ctggacatca accggctgtc cgactacgat gtggaccata tcgtgcctca     3900

gagctttctg aaggacgact ccatcgacaa caaggtgctg accagaagcg acaagaaccg     3960

gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg     4020

gcagctgctg aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga     4080

gagaggcggc ctgagcgaac tggataaggc cggcttcatc aagagacagc tggtggaaac     4140

ccggcagatc acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga     4200

cgagaatgac aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc     4260

cgatttccgg aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc     4320

ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct     4380

ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa     4440

gagcgagcag gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa     4500

ctttttcaag accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga     4560

gacaaacggc gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg     4620

gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg     4680

cttcagcaaa gagtctatcc tgcccaagag gaacagcgat aagctgatcg ccagaaagaa     4740

ggactgggac cctaagaagt acggcggctt cgacagcccc accgtggcct attctgtgct     4800

ggtggtggcc aaagtggaaa agggcaagtc caagaaactg aagagtgtga aagagctgct     4860

ggggatcacc atcatggaaa gaagcagctt cgagaagaat cccatcgact ttctggaagc     4920

caagggctac aaagaagtga aaaaggacct gatcatcaag ctgcctaagt actccctgtt     4980

cgagctggaa aacggccgga agagaatgct ggcctctgcc ggcgaactgc agaagggaaa     5040

cgaactggcc ctgccctcca aatatgtgaa cttcctgtac ctggccagcc actatgagaa     5100

gctgaagggc tcccccgagg ataatgagca gaaacagctg tttgtggaac agcacaagca     5160

ctacctggac gagatcatcg agcagatcag cgagttctcc aagagagtga tcctggccga     5220

cgctaatctg gacaaagtgc tgtccgccta caacaagcac cgggataagc ccatcagaga     5280

gcaggccgag aatatcatcc acctgtttac cctgaccaat ctgggagccc ctgccgcctt     5340

caagtacttt gacaccacca tcgaccggaa gaggtacacc agcaccaaag aggtgctgga     5400

cgccaccctg atccaccaga gcatcaccgg cctgtacgag acacggatcg acctgtctca     5460

gctgggaggc gacaaaaggc cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa     5520

ggaattctaa ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct     5580

gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt     5640

tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg     5700

ggtggggtgg ggcaggacag caagggggag gattgggaag agaatagcag gcatgctggg     5760

gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg     5820

ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca     5880

gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg     5940

catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg     6000

gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg     6060

ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc     6120

cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc     6180

tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga     6240

cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa     6300

ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga     6360

tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca     6420

aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat     6480

agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc     6540

tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt     6600

tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat     6660

aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg     6720

tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga     6780

gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac     6840

atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc     6900

cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca     6960

tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc     7020

caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg     7080

ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac     7140

cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca     7200

taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg     7260

agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac     7320

cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg     7380

caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat     7440

taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg     7500

ctggctggtt tattgctgat aaatctggag ccggtgagcg tggaagccgc ggtatcattg     7560

cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc     7620

aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc     7680

attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt     7740

tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt     7800

aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt     7860

gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag     7920

cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca     7980

gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca     8040

agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg     8100

ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg     8160

cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct     8220

acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga     8280

gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc     8340

ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg     8400

agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg     8460

cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt                  8508


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA C35R

<400>  29
gacaaacact ccaccagcat                                                   20


<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA C35L

<400>  30
gcccaatcca tcttctactc t                                                 21


<210>  31
<211>  8088
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCRISPR/Cas9n(D10A)DN

<400>  31
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca      240

gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat      300

taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc      360

acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg      420

gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg      480

gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag      540

gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgac      600

gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac      660

tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt      720

agctgagcaa gaggtaaggg tttaagggat ggttggttgg tggggtatta atgtttaatt      780

acctggagca cctgcctgaa atcacttttt ttcaggttgg accggtgcca ccatggacta      840

taaggaccac gacggagact acaaggatca tgatattgat tacaaagacg atgacgataa      900

gatggcccca aagaagaagc ggaaggtcgg tatccacgga gtcccagcag ccgacaagaa      960

gtacagcatc ggcctggcca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga     1020

gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa     1080

gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct     1140

gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga     1200

gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc     1260

cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga     1320

cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga     1380

cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt     1440

ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct     1500

gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag     1560

cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa     1620

tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggcaacc tgattgccct     1680

gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact     1740

gcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga     1800

ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga     1860

catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag     1920

atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc     1980

tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga     2040

cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga     2100

cggcaccgag gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac     2160

cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg     2220

gcggcaggaa gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct     2280

gaccttccgc atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg     2340

gatgaccaga aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa     2400

gggcgcttcc gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa     2460

cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct     2520

gaccaaagtg aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca     2580

gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct     2640

gaaagaggac tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga     2700

agatcggttc aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa     2760

ggacttcctg gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac     2820

actgtttgag gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga     2880

cgacaaagtg atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg     2940

gaagctgatc aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa     3000

gtccgacggc ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt     3060

taaagaggac atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat     3120

tgccaatctg gccggcagcc ccgccattaa gaagggcatc ctgcagacag tgaaggtggt     3180

ggacgagctc gtgaaagtga tgggccggca caagcccgag aacatcgtga tcgaaatggc     3240

cagagagaac cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat     3300

cgaagagggc atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac     3360

ccagctgcag aacgagaagc tgtacctgta ctacctgcag aatgggcggg atatgtacgt     3420

ggaccaggaa ctggacatca accggctgtc cgactacgat gtggaccata tcgtgcctca     3480

gagctttctg aaggacgact ccatcgacaa caaggtgctg accagaagcg acaagaaccg     3540

gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg     3600

gcagctgctg aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga     3660

gagaggcggc ctgagcgaac tggataaggc cggcttcatc aagagacagc tggtggaaac     3720

ccggcagatc acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga     3780

cgagaatgac aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc     3840

cgatttccgg aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc     3900

ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct     3960

ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa     4020

gagcgagcag gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa     4080

ctttttcaag accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga     4140

gacaaacggc gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg     4200

gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg     4260

cttcagcaaa gagtctatcc tgcccaagag gaacagcgat aagctgatcg ccagaaagaa     4320

ggactgggac cctaagaagt acggcggctt cgacagcccc accgtggcct attctgtgct     4380

ggtggtggcc aaagtggaaa agggcaagtc caagaaactg aagagtgtga aagagctgct     4440

ggggatcacc atcatggaaa gaagcagctt cgagaagaat cccatcgact ttctggaagc     4500

caagggctac aaagaagtga aaaaggacct gatcatcaag ctgcctaagt actccctgtt     4560

cgagctggaa aacggccgga agagaatgct ggcctctgcc ggcgaactgc agaagggaaa     4620

cgaactggcc ctgccctcca aatatgtgaa cttcctgtac ctggccagcc actatgagaa     4680

gctgaagggc tcccccgagg ataatgagca gaaacagctg tttgtggaac agcacaagca     4740

ctacctggac gagatcatcg agcagatcag cgagttctcc aagagagtga tcctggccga     4800

cgctaatctg gacaaagtgc tgtccgccta caacaagcac cgggataagc ccatcagaga     4860

gcaggccgag aatatcatcc acctgtttac cctgaccaat ctgggagccc ctgccgcctt     4920

caagtacttt gacaccacca tcgaccggaa gaggtacacc agcaccaaag aggtgctgga     4980

cgccaccctg atccaccaga gcatcaccgg cctgtacgag acacggatcg acctgtctca     5040

gctgggaggc gacaaaaggc cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa     5100

ggaattctaa ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct     5160

gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt     5220

tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg     5280

ggtggggtgg ggcaggacag caagggggag gattgggaag agaatagcag gcatgctggg     5340

gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg     5400

ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca     5460

gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg     5520

catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg     5580

gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg     5640

ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc     5700

cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc     5760

tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga     5820

cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa     5880

ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga     5940

tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca     6000

aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat     6060

agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc     6120

tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt     6180

tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat     6240

aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg     6300

tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga     6360

gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac     6420

atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc     6480

cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca     6540

tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc     6600

caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg     6660

ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac     6720

cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca     6780

taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg     6840

agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac     6900

cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg     6960

caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat     7020

taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg     7080

ctggctggtt tattgctgat aaatctggag ccggtgagcg tggaagccgc ggtatcattg     7140

cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc     7200

aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc     7260

attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt     7320

tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt     7380

aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt     7440

gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag     7500

cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca     7560

gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca     7620

agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg     7680

ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg     7740

cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct     7800

acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga     7860

gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc     7920

ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg     7980

agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg     8040

cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt                  8088


<210>  32
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GJB-EX2-FU-f


<220>
<221>  primer_bind
<222>  (1)..(44)

<400>  32
tgactaattt tttttattta tgcagagcaa accgcccaga gtag                        44


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GJB-EX2-r


<220>
<221>  primer_bind
<222>  (1)..(20)

<400>  33
atgtctccgg taggccacgt                                                   20


<210>  34
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AF-INTRON-f


<220>
<221>  primer_bind
<222>  (1)..(23)

<400>  34
aggtaagtta ggcagggata ttc                                               23


<210>  35
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AF-INTRON-r


<220>
<221>  primer_bind
<222>  (1)..(27)

<400>  35
ctgcataaat aaaaaaaatt agtcagc                                           27


<210>  36
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M3-GJ-f


<220>
<221>  primer_bind
<222>  (1)..(38)

<400>  36
gaagttcatc aagggcagct cactcaaagg cggtaata                               38


<210>  37
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M3-GJ-r


<220>
<221>  primer_bind
<222>  (1)..(36)

<400>  37
tcggtgaatt taaaactcga ggtgaagacg aaaggg                                 36


<210>  38
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GJB-35delG-f


<220>
<221>  primer_bind
<222>  (1)..(21)

<400>  38
ctgggggtgt gaacaaacac t                                                 21


<210>  39
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GJB-35delG-r


<220>
<221>  primer_bind
<222>  (1)..(21)

<400>  39
tttgttcaca cccccaggat c                                                 21


<210>  40
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GJB-109G/A-f


<220>
<221>  primer_bind
<222>  (1)..(22)

<400>  40
tgatcctcat tgtggctgca aa                                                22


<210>  41
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GJB-109G/A-r


<220>
<221>  primer_bind
<222>  (1)..(23)

<400>  41
gcagccacaa tgaggatcat aat                                               23


