                         SEQUENCE LISTING

<110>  The Broad Institute, Inc.
 
<120>  METHODS OF TREATING AUTISM SPECTRUM DISORDERS

<130>  01180-0001-00PCT

<150>  US 62559765
<151>  2017-09-18

<150>  US 62582472
<151>  2017-11-07

<150>  US 62435986
<151>  2016-12-19

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA1 designed for Kctd13

<400>  1
gccggctgcg gccgaatgct                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA2 designed for Kctd13

<400>  2
aggggcttca gactgtacga                                                   20


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA3 designed for Kctd13

<400>  3
caccacgctg cgcaccctca                                                   20


<210>  4
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kctd13 forward primer 1

<400>  4
cggagtagct gtggagagtg g                                                 21


<210>  5
<211>  125
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kctd13 reverse primer 1

<400>  5
aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa       60

cttgctattt ctagctctaa aacagcattc ggccgcagcc ggcggtgttt cgtcctttcc      120

acaag                                                                  125


<210>  6
<211>  126
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kctd13 reverse primer 2

<400>  6
aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa       60

cttgctattt ctagctctaa aactcgtaca gtctgaagcc cctcggtgtt tcgtcctttc      120

cacaag                                                                 126


<210>  7
<211>  126
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kctd13 reverse primer 3

<400>  7
aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa       60

cttgctattt ctagctctaa aactgagggt gcgcagcgtg gtgcggtgtt tcgtcctttc      120

cacaag                                                                 126


<210>  8
<211>  121
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse sgRNA IVT primer 1

<400>  8
aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc ttattttaac       60

ttgctatttc tagctctaaa acagcattcg gccgcagccg gccctatagt gagtcgtatt      120

a                                                                      121


<210>  9
<211>  122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse sgRNA IVT primer 2

<400>  9
aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc ttattttaac       60

ttgctatttc tagctctaaa actcgtacag tctgaagccc ctccctatag tgagtcgtat      120

ta                                                                     122


<210>  10
<211>  122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse sgRNA IVT primer 3

<400>  10
aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc ttattttaac       60

ttgctatttc tagctctaaa actgagggtg cgcagcgtgg tgccctatag tgagtcgtat      120

ta                                                                     122


<210>  11
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminal peptide of KCTD13

<400>  11

Cys Val Arg Arg His Ile Thr His Asp Glu Arg Pro His Gly Gln Gln 
1               5                   10                  15      


Ile Val Phe Lys Asp 
            20      


<210>  12
<211>  456
<212>  PRT
<213>  Homo sapiens

<400>  12

Met Ala Phe Ala Glu Thr Tyr Pro Ala Ala Ser Ser Leu Pro Asn Gly 
1               5                   10                  15      


Asp Cys Gly Arg Pro Arg Ala Arg Pro Gly Gly Asn Arg Val Thr Val 
            20                  25                  30          


Val Leu Gly Ala Gln Trp Gly Asp Glu Gly Lys Gly Lys Val Val Asp 
        35                  40                  45              


Leu Leu Ala Gln Asp Ala Asp Ile Val Cys Arg Cys Gln Gly Gly Asn 
    50                  55                  60                  


Asn Ala Gly His Thr Val Val Val Asp Ser Val Glu Tyr Asp Phe His 
65                  70                  75                  80  


Leu Leu Pro Ser Gly Ile Ile Asn Pro Asn Val Thr Ala Phe Ile Gly 
                85                  90                  95      


Asn Gly Val Val Ile His Leu Pro Gly Leu Phe Glu Glu Ala Glu Lys 
            100                 105                 110         


Asn Val Gln Lys Gly Lys Gly Leu Glu Gly Trp Glu Lys Arg Leu Ile 
        115                 120                 125             


Ile Ser Asp Arg Ala His Ile Val Phe Asp Phe His Gln Ala Ala Asp 
    130                 135                 140                 


Gly Ile Gln Glu Gln Gln Arg Gln Glu Gln Ala Gly Lys Asn Leu Gly 
145                 150                 155                 160 


Thr Thr Lys Lys Gly Ile Gly Pro Val Tyr Ser Ser Lys Ala Ala Arg 
                165                 170                 175     


Ser Gly Leu Arg Met Cys Asp Leu Val Ser Asp Phe Asp Gly Phe Ser 
            180                 185                 190         


Glu Arg Phe Lys Val Leu Ala Asn Gln Tyr Lys Ser Ile Tyr Pro Thr 
        195                 200                 205             


Leu Glu Ile Asp Ile Glu Gly Glu Leu Gln Lys Leu Lys Gly Tyr Met 
    210                 215                 220                 


Glu Lys Ile Lys Pro Met Val Arg Asp Gly Val Tyr Phe Leu Tyr Glu 
225                 230                 235                 240 


Ala Leu His Gly Pro Pro Lys Lys Ile Leu Val Glu Gly Ala Asn Ala 
                245                 250                 255     


Ala Leu Leu Asp Ile Asp Phe Gly Thr Tyr Pro Phe Val Thr Ser Ser 
            260                 265                 270         


Asn Cys Thr Val Gly Gly Val Cys Thr Gly Leu Gly Met Pro Pro Gln 
        275                 280                 285             


Asn Val Gly Glu Val Tyr Gly Val Val Lys Ala Tyr Thr Thr Arg Val 
    290                 295                 300                 


Gly Ile Gly Ala Phe Pro Thr Glu Gln Asp Asn Glu Ile Gly Glu Leu 
305                 310                 315                 320 


Leu Gln Thr Arg Gly Arg Glu Phe Gly Val Thr Thr Gly Arg Lys Arg 
                325                 330                 335     


Arg Cys Gly Trp Leu Asp Leu Val Leu Leu Lys Tyr Ala His Met Ile 
            340                 345                 350         


Asn Gly Phe Thr Ala Leu Ala Leu Thr Lys Leu Asp Ile Leu Asp Met 
        355                 360                 365             


Phe Thr Glu Ile Lys Val Gly Val Ala Tyr Lys Leu Asp Gly Glu Ile 
    370                 375                 380                 


Ile Pro His Ile Pro Ala Asn Gln Glu Val Leu Asn Lys Val Glu Val 
385                 390                 395                 400 


Gln Tyr Lys Thr Leu Pro Gly Trp Asn Thr Asp Ile Ser Asn Ala Arg 
                405                 410                 415     


Ala Phe Lys Glu Leu Pro Val Asn Ala Gln Asn Tyr Val Arg Phe Ile 
            420                 425                 430         


Glu Asp Glu Leu Gln Ile Pro Val Lys Trp Ile Gly Val Gly Lys Ser 
        435                 440                 445             


Arg Glu Ser Met Ile Gln Leu Phe 
    450                 455     


<210>  13
<211>  484
<212>  PRT
<213>  Homo sapiens

<400>  13

Met Ala Ala Gly Gly Asp His Gly Ser Pro Asp Ser Tyr Arg Ser Pro 
1               5                   10                  15      


Leu Ala Ser Arg Tyr Ala Ser Pro Glu Met Cys Phe Val Phe Ser Asp 
            20                  25                  30          


Arg Tyr Lys Phe Arg Thr Trp Arg Gln Leu Trp Leu Trp Leu Ala Glu 
        35                  40                  45              


Ala Glu Gln Thr Leu Gly Leu Pro Ile Thr Asp Glu Gln Ile Gln Glu 
    50                  55                  60                  


Met Lys Ser Asn Leu Glu Asn Ile Asp Phe Lys Met Ala Ala Glu Glu 
65                  70                  75                  80  


Glu Lys Arg Leu Arg His Asp Val Met Ala His Val His Thr Phe Gly 
                85                  90                  95      


His Cys Cys Pro Lys Ala Ala Gly Ile Ile His Leu Gly Ala Thr Ser 
            100                 105                 110         


Cys Tyr Val Gly Asp Asn Thr Asp Leu Ile Ile Leu Arg Asn Ala Leu 
        115                 120                 125             


Asp Leu Leu Leu Pro Lys Leu Ala Arg Val Ile Ser Arg Leu Ala Asp 
    130                 135                 140                 


Phe Ala Lys Glu Arg Ala Ser Leu Pro Thr Leu Gly Phe Thr His Phe 
145                 150                 155                 160 


Gln Pro Ala Gln Leu Thr Thr Val Gly Lys Arg Cys Cys Leu Trp Ile 
                165                 170                 175     


Gln Asp Leu Cys Met Asp Leu Gln Asn Leu Lys Arg Val Arg Asp Asp 
            180                 185                 190         


Leu Arg Phe Arg Gly Val Lys Gly Thr Thr Gly Thr Gln Ala Ser Phe 
        195                 200                 205             


Leu Gln Leu Phe Glu Gly Asp Asp His Lys Val Glu Gln Leu Asp Lys 
    210                 215                 220                 


Met Val Thr Glu Lys Ala Gly Phe Lys Arg Ala Phe Ile Ile Thr Gly 
225                 230                 235                 240 


Gln Thr Tyr Thr Arg Lys Val Asp Ile Glu Val Leu Ser Val Leu Ala 
                245                 250                 255     


Ser Leu Gly Ala Ser Val His Lys Ile Cys Thr Asp Ile Arg Leu Leu 
            260                 265                 270         


Ala Asn Leu Lys Glu Met Glu Glu Pro Phe Glu Lys Gln Gln Ile Gly 
        275                 280                 285             


Ser Ser Ala Met Pro Tyr Lys Arg Asn Pro Met Arg Ser Glu Arg Cys 
    290                 295                 300                 


Cys Ser Leu Ala Arg His Leu Met Thr Leu Val Met Asp Pro Leu Gln 
305                 310                 315                 320 


Thr Ala Ser Val Gln Trp Phe Glu Arg Thr Leu Asp Asp Ser Ala Asn 
                325                 330                 335     


Arg Arg Ile Cys Leu Ala Glu Ala Phe Leu Thr Ala Asp Thr Ile Leu 
            340                 345                 350         


Asn Thr Leu Gln Asn Ile Ser Glu Gly Leu Val Val Tyr Pro Lys Val 
        355                 360                 365             


Ile Glu Arg Arg Ile Arg Gln Glu Leu Pro Phe Met Ala Thr Glu Asn 
    370                 375                 380                 


Ile Ile Met Ala Met Val Lys Ala Gly Gly Ser Arg Gln Asp Cys His 
385                 390                 395                 400 


Glu Lys Ile Arg Val Leu Ser Gln Gln Ala Ala Ser Val Val Lys Gln 
                405                 410                 415     


Glu Gly Gly Asp Asn Asp Leu Ile Glu Arg Ile Gln Val Asp Ala Tyr 
            420                 425                 430         


Phe Ser Pro Ile His Ser Gln Leu Asp His Leu Leu Asp Pro Ser Ser 
        435                 440                 445             


Phe Thr Gly Arg Ala Ser Gln Gln Val Gln Arg Phe Leu Glu Glu Glu 
    450                 455                 460                 


Val Tyr Pro Leu Leu Lys Pro Tyr Glu Ser Val Met Lys Val Lys Ala 
465                 470                 475                 480 


Glu Leu Cys Leu 
                


<210>  14
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genotyping primer Kctd13 F 47_64nt

<400>  14
tccgctcact ggcatgtc                                                     18


<210>  15
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genotyping primer Kctd13 R 47_64nt

<400>  15
cacactcgag gggctagg                                                     18


<210>  16
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genotyping probe Kctd13 probe 47_64nt


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n = 56-FAM

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  n = ZEN

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  n = 3IABkFQ

<400>  16
ntgcggccga natgcttgga gtccn                                             25


<210>  17
<211>  559
<212>  PRT
<213>  Homo sapiens

<400>  17

Met Arg Arg Leu Ser Ser Trp Arg Lys Met Ala Thr Ala Glu Lys Gln 
1               5                   10                  15      


Lys His Asp Gly Arg Val Lys Ile Gly His Tyr Ile Leu Gly Asp Thr 
            20                  25                  30          


Leu Gly Val Gly Thr Phe Gly Lys Val Lys Val Gly Lys His Glu Leu 
        35                  40                  45              


Thr Gly His Lys Val Ala Val Lys Ile Leu Asn Arg Gln Lys Ile Arg 
    50                  55                  60                  


Ser Leu Asp Val Val Gly Lys Ile Arg Arg Glu Ile Gln Asn Leu Lys 
65                  70                  75                  80  


Leu Phe Arg His Pro His Ile Ile Lys Leu Tyr Gln Val Ile Ser Thr 
                85                  90                  95      


Pro Ser Asp Ile Phe Met Val Met Glu Tyr Val Ser Gly Gly Glu Leu 
            100                 105                 110         


Phe Asp Tyr Ile Cys Lys Asn Gly Arg Leu Asp Glu Lys Glu Ser Arg 
        115                 120                 125             


Arg Leu Phe Gln Gln Ile Leu Ser Gly Val Asp Tyr Cys His Arg His 
    130                 135                 140                 


Met Val Val His Arg Asp Leu Lys Pro Glu Asn Val Leu Leu Asp Ala 
145                 150                 155                 160 


His Met Asn Ala Lys Ile Ala Asp Phe Gly Leu Ser Asn Met Met Ser 
                165                 170                 175     


Asp Gly Glu Phe Leu Arg Thr Ser Cys Gly Ser Pro Asn Tyr Ala Ala 
            180                 185                 190         


Pro Glu Val Ile Ser Gly Arg Leu Tyr Ala Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Trp Ser Ser Gly Val Ile Leu Tyr Ala Leu Leu Cys Gly Thr Leu Pro 
    210                 215                 220                 


Phe Asp Asp Asp His Val Pro Thr Leu Phe Lys Lys Ile Cys Asp Gly 
225                 230                 235                 240 


Ile Phe Tyr Thr Pro Gln Tyr Leu Asn Pro Ser Val Ile Ser Leu Leu 
                245                 250                 255     


Lys His Met Leu Gln Val Asp Pro Met Lys Arg Ala Thr Ile Lys Asp 
            260                 265                 270         


Ile Arg Glu His Glu Trp Phe Lys Gln Asp Leu Pro Lys Tyr Leu Phe 
        275                 280                 285             


Pro Glu Asp Pro Ser Tyr Ser Ser Thr Met Ile Asp Asp Glu Ala Leu 
    290                 295                 300                 


Lys Glu Val Cys Glu Lys Phe Glu Cys Ser Glu Glu Glu Val Leu Ser 
305                 310                 315                 320 


Cys Leu Tyr Asn Arg Asn His Gln Asp Pro Leu Ala Val Ala Tyr His 
                325                 330                 335     


Leu Ile Ile Asp Asn Arg Arg Ile Met Asn Glu Ala Lys Asp Phe Tyr 
            340                 345                 350         


Leu Ala Thr Ser Pro Pro Asp Ser Phe Leu Asp Asp His His Leu Thr 
        355                 360                 365             


Arg Pro His Pro Glu Arg Val Pro Phe Leu Val Ala Glu Thr Pro Arg 
    370                 375                 380                 


Ala Arg His Thr Leu Asp Glu Leu Asn Pro Gln Lys Ser Lys His Gln 
385                 390                 395                 400 


Gly Val Arg Lys Ala Lys Trp His Leu Gly Ile Arg Ser Gln Ser Arg 
                405                 410                 415     


Pro Asn Asp Ile Met Ala Glu Val Cys Arg Ala Ile Lys Gln Leu Asp 
            420                 425                 430         


Tyr Glu Trp Lys Val Val Asn Pro Tyr Tyr Leu Arg Val Arg Arg Lys 
        435                 440                 445             


Asn Pro Val Thr Ser Thr Tyr Ser Lys Met Ser Leu Gln Leu Tyr Gln 
    450                 455                 460                 


Val Asp Ser Arg Thr Tyr Leu Leu Asp Phe Arg Ser Ile Asp Asp Glu 
465                 470                 475                 480 


Ile Thr Glu Ala Lys Ser Gly Thr Ala Thr Pro Gln Arg Ser Gly Ser 
                485                 490                 495     


Val Ser Asn Tyr Arg Ser Cys Gln Arg Ser Asp Ser Asp Ala Glu Ala 
            500                 505                 510         


Gln Gly Lys Ser Ser Glu Val Ser Leu Thr Ser Ser Val Thr Ser Leu 
        515                 520                 525             


Asp Ser Ser Pro Val Asp Leu Thr Pro Arg Pro Gly Ser His Thr Ile 
    530                 535                 540                 


Glu Phe Phe Glu Met Cys Ala Asn Leu Ile Lys Ile Leu Ala Gln 
545                 550                 555                 


<210>  18
<211>  270
<212>  PRT
<213>  Homo sapiens

<400>  18

Met Gly Asn Thr Ser Ser Glu Arg Ala Ala Leu Glu Arg His Gly Gly 
1               5                   10                  15      


His Lys Thr Pro Arg Arg Asp Ser Ser Gly Gly Thr Lys Asp Gly Asp 
            20                  25                  30          


Arg Pro Lys Ile Leu Met Asp Ser Pro Glu Asp Ala Asp Leu Phe His 
        35                  40                  45              


Ser Glu Glu Ile Lys Ala Pro Glu Lys Glu Glu Phe Leu Ala Trp Gln 
    50                  55                  60                  


His Asp Leu Glu Val Asn Asp Lys Ala Pro Ala Gln Ala Arg Pro Thr 
65                  70                  75                  80  


Val Phe Arg Trp Thr Gly Gly Gly Lys Glu Val Tyr Leu Ser Gly Ser 
                85                  90                  95      


Phe Asn Asn Trp Ser Lys Leu Pro Leu Thr Arg Ser His Asn Asn Phe 
            100                 105                 110         


Val Ala Ile Leu Asp Leu Pro Glu Gly Glu His Gln Tyr Lys Phe Phe 
        115                 120                 125             


Val Asp Gly Gln Trp Thr His Asp Pro Ser Glu Pro Ile Val Thr Ser 
    130                 135                 140                 


Gln Leu Gly Thr Val Asn Asn Ile Ile Gln Val Lys Lys Thr Asp Phe 
145                 150                 155                 160 


Glu Val Phe Asp Ala Leu Met Val Asp Ser Gln Lys Cys Ser Asp Val 
                165                 170                 175     


Ser Glu Leu Ser Ser Ser Pro Pro Gly Pro Tyr His Gln Glu Pro Tyr 
            180                 185                 190         


Val Cys Lys Pro Glu Glu Arg Phe Arg Ala Pro Pro Ile Leu Pro Pro 
        195                 200                 205             


His Leu Leu Gln Val Ile Leu Asn Lys Asp Thr Gly Ile Ser Cys Asp 
    210                 215                 220                 


Pro Ala Leu Leu Pro Glu Pro Asn His Val Met Leu Asn His Leu Tyr 
225                 230                 235                 240 


Ala Leu Ser Ile Lys Asp Gly Val Met Val Leu Ser Ala Thr His Arg 
                245                 250                 255     


Tyr Lys Lys Lys Tyr Val Thr Thr Leu Leu Tyr Lys Pro Ile 
            260                 265                 270 


<210>  19
<211>  331
<212>  PRT
<213>  Homo sapiens

<400>  19

Met Glu Thr Val Ile Ser Ser Asp Ser Ser Pro Ala Val Glu Asn Glu 
1               5                   10                  15      


His Pro Gln Glu Thr Pro Glu Ser Asn Asn Ser Val Tyr Thr Ser Phe 
            20                  25                  30          


Met Lys Ser His Arg Cys Tyr Asp Leu Ile Pro Thr Ser Ser Lys Leu 
        35                  40                  45              


Val Val Phe Asp Thr Ser Leu Gln Val Lys Lys Ala Phe Phe Ala Leu 
    50                  55                  60                  


Val Thr Asn Gly Val Arg Ala Ala Pro Leu Trp Asp Ser Lys Lys Gln 
65                  70                  75                  80  


Ser Phe Val Gly Met Leu Thr Ile Thr Asp Phe Ile Asn Ile Leu His 
                85                  90                  95      


Arg Tyr Tyr Lys Ser Ala Leu Val Gln Ile Tyr Glu Leu Glu Glu His 
            100                 105                 110         


Lys Ile Glu Thr Trp Arg Glu Val Tyr Leu Gln Asp Ser Phe Lys Pro 
        115                 120                 125             


Leu Val Cys Ile Ser Pro Asn Ala Ser Leu Phe Asp Ala Val Ser Ser 
    130                 135                 140                 


Leu Ile Arg Asn Lys Ile His Arg Leu Pro Val Ile Asp Pro Glu Ser 
145                 150                 155                 160 


Gly Asn Thr Leu Tyr Ile Leu Thr His Lys Arg Ile Leu Lys Phe Leu 
                165                 170                 175     


Lys Leu Phe Ile Thr Glu Phe Pro Lys Pro Glu Phe Met Ser Lys Ser 
            180                 185                 190         


Leu Glu Glu Leu Gln Ile Gly Thr Tyr Ala Asn Ile Ala Met Val Arg 
        195                 200                 205             


Thr Thr Thr Pro Val Tyr Val Ala Leu Gly Ile Phe Val Gln His Arg 
    210                 215                 220                 


Val Ser Ala Leu Pro Val Val Asp Glu Lys Gly Arg Val Val Asp Ile 
225                 230                 235                 240 


Tyr Ser Lys Phe Asp Val Ile Asn Leu Ala Ala Glu Lys Thr Tyr Asn 
                245                 250                 255     


Asn Leu Asp Val Ser Val Thr Lys Ala Leu Gln His Arg Ser His Tyr 
            260                 265                 270         


Phe Glu Gly Val Leu Lys Cys Tyr Leu His Glu Thr Leu Glu Thr Ile 
        275                 280                 285             


Ile Asn Arg Leu Val Glu Ala Glu Val His Arg Leu Val Val Val Asp 
    290                 295                 300                 


Glu Asn Asp Val Val Lys Gly Ile Val Ser Leu Ser Asp Ile Leu Gln 
305                 310                 315                 320 


Ala Leu Val Leu Thr Gly Gly Glu Lys Lys Pro 
                325                 330     


