                         SEQUENCE LISTING

<110>  PETIVA PVT. LTD.
 
<120>  D-PSICOSE 3-EPIMERASE MUTANT AND USES THEREOF

<130>  IP36784

<150>  201641044105
<151>  2016-12-23

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  870
<212>  DNA
<213>  Agrobacterium tumefaciens

<400>  1
atgaaacacg gcatctatta ttcttactgg gaacatgagt ggagcgccaa gttcggtccc       60

tatatcgaga aggtcgccaa gctcggtttc gacatcatcg aagtcgccgc ccaccatatc      120

aacgaataca gcgacgccga actcgcgacc atcaggaaga gcgcgaagga taacggcatc      180

atcctcaccg ccggcatcgg tccgtcgaaa accaagaacc tgtcgtcgga agatgctgcg      240

gtgcgtgcgg ccggcaaggc gttctttgaa agaacccttt cgaacgtcgc caagctcgat      300

atccacacca tcggcggcgc attgcattcc tattggccaa tcgattattc gcagcccgtc      360

gacaaggcag gcgattatgc gcgcggcgtc gagggtatca acggcattgc cgatttcgcc      420

aatgatctcg gcatcaacct gtgcatcgaa gtcctcaacc gctttgaaaa ccacgtcctc      480

aacacggcgg cggaaggcgt cgcttttgtg aaggatgtcg gcaagaacaa tgtgaaagtc      540

atgctggata ccttccacat gaacatcgag gaagacagtt tcggtgacgc catccgcacg      600

gccggcccgc ttctggggca cttccatacc ggtgaaagca atcgccgcgt accgggcaag      660

ggcagaatgc cgtggcacga aatcggcctt gcgctgcgtg atatcaacta caccggcgcg      720

gtaatcatgg agcctttcgt caagacaggc ggcaccatcg gctcggatat caaggtgtgg      780

cgcgacctga gcggtggcgc cgacatcgcg aaaatggatg aagatgcccg caatgcgctg      840

gcattctccc gcttcgttct tggtggctga                                       870


<210>  2
<211>  870
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified nucleotide sequence encoding D-psicose 3-epimerase of 
       Agrobacterium tumefaciens

<400>  2
atgaaacatg gtatctatta cagttattgg gaacacgaat ggagcgcgaa atttggtccg       60

tacattgaaa aagttgccaa actgggcttc gatattatcg aagtggcggc ccatcacatc      120

aatgaatata gtgatgccga actggcaacc attcgtaaaa gcgcaaaaga taatggtatt      180

atcctgaccg cgggcatcgg tccgagcaaa acgaaaaacc tgagctctga agatgcagcg      240

gttcgtgccg caggcaaagc atttttcgaa cgcaccctgt ctaatgtggc caaactggat      300

attcatacga tcggcggtgc gctgcactct tattggccga ttgattacag tcagccggtt      360

gataaagccg gtgattacgc acgtggcgtg gaaggtatta acggcatcgc ggattttgcc      420

aatgatctgg gcattaacct gtgcatcgaa gttctgaacc gctttgaaaa tcatgtgctg      480

aacaccgcgg ccgagggtgt ggcgttcgtt aaagatgtgg gcaaaaacaa tgtgaaagtt      540

atgctggata cgtttcacat gaatatcgaa gaagattctt tcggtgatgc aattcgtacc      600

gcaggtccgc tgctgggtca ttttcacacg ggcgaaagta atcgtcgcgt tccgggcaaa      660

ggtcgtatgc cgtggcatga aattggtctg gccctgcgcg atatcaacta taccggcgca      720

gttattatgg aaccgttcgt gaaaaccggc ggtacgattg gcagcgatat caaagtttgg      780

cgcgatctgt ctggcggtgc agatattgcg aaaatggatg aagatgcgcg taacgcactg      840

gcgtttagcc gcttcgtgct gggcggttaa                                       870


<210>  3
<211>  870
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of mutant D-psicose 3-epimerase

<400>  3
atgaaacatg gtatctacta tgcatactgg gaacaggagt gggaagctaa atttggtcca       60

tatattgaaa aggtcgccaa gcttggcttc gatattctag aagtggcggc ccatcacatc      120

aatgaatata gtgatgccga actggcaacc attcgtaaaa gcgcaaaaga taatggtatt      180

atcctgacag ctggcatcgg cccgagcaaa acgaaaaacc tgagctctga agatgcagcg      240

gttcgtgccg caggcaaagc atttttcgaa cgcaccctgt ctaatgtggc caaactggat      300

attcatacga tcggcggtgc gctgcactct tattggccga ttgattacag tcagccggtt      360

gataaagccg gtgattacgc acgtggcgtg gaaggtatta acggcatcgc ggattttgcc      420

aatgatctgg gcattaacct gtgcatcgaa gttctgaacc gctttgaaaa tcatgtgctg      480

aacaccgcgg ccgagggtgt ggcgttcgtt aaagatgtgg gcaaaaacaa tgtgaaagtt      540

atgctggata cgtttcacat gaatatcgaa gaagattctt tcggtgatgc aattcgtact      600

gcaggtccgc tgctgggtca ttttcacacg ggcgaaagta atcgtcgcgt cccgggcaaa      660

ggtcgtatgc cgtggcatga aattggtctg gccctgcgcg atatcaacta taccggcgca      720

gttattatgg aaccgttcgt gaaaaccggc ggtacgattg gcagcgatat caaagtttgg      780

cgcgatctgt ctggcggtgc agatattgcg aaaatggatg aagatgcgcg taacgcactg      840

gcgtttagcc gcttcgtgct gggcggttaa                                       870


<210>  4
<211>  289
<212>  PRT
<213>  Agrobacterium tumefaciens

<400>  4

Met Lys His Gly Ile Tyr Tyr Ser Tyr Trp Glu His Glu Trp Ser Ala 
1               5                   10                  15      


Lys Phe Gly Pro Tyr Ile Glu Lys Val Ala Lys Leu Gly Phe Asp Ile 
            20                  25                  30          


Ile Glu Val Ala Ala His His Ile Asn Glu Tyr Ser Asp Ala Glu Leu 
        35                  40                  45              


Ala Thr Ile Arg Lys Ser Ala Lys Asp Asn Gly Ile Ile Leu Thr Ala 
    50                  55                  60                  


Gly Ile Gly Pro Ser Lys Thr Lys Asn Leu Ser Ser Glu Asp Ala Ala 
65                  70                  75                  80  


Val Arg Ala Ala Gly Lys Ala Phe Phe Glu Arg Thr Leu Ser Asn Val 
                85                  90                  95      


Ala Lys Leu Asp Ile His Thr Ile Gly Gly Ala Leu His Ser Tyr Trp 
            100                 105                 110         


Pro Ile Asp Tyr Ser Gln Pro Val Asp Lys Ala Gly Asp Tyr Ala Arg 
        115                 120                 125             


Gly Val Glu Gly Ile Asn Gly Ile Ala Asp Phe Ala Asn Asp Leu Gly 
    130                 135                 140                 


Ile Asn Leu Cys Ile Glu Val Leu Asn Arg Phe Glu Asn His Val Leu 
145                 150                 155                 160 


Asn Thr Ala Ala Glu Gly Val Ala Phe Val Lys Asp Val Gly Lys Asn 
                165                 170                 175     


Asn Val Lys Val Met Leu Asp Thr Phe His Met Asn Ile Glu Glu Asp 
            180                 185                 190         


Ser Phe Gly Asp Ala Ile Arg Thr Ala Gly Pro Leu Leu Gly His Phe 
        195                 200                 205             


His Thr Gly Glu Ser Asn Arg Arg Val Pro Gly Lys Gly Arg Met Pro 
    210                 215                 220                 


Trp His Glu Ile Gly Leu Ala Leu Arg Asp Ile Asn Tyr Thr Gly Ala 
225                 230                 235                 240 


Val Ile Met Glu Pro Phe Val Lys Thr Gly Gly Thr Ile Gly Ser Asp 
                245                 250                 255     


Ile Lys Val Trp Arg Asp Leu Ser Gly Gly Ala Asp Ile Ala Lys Met 
            260                 265                 270         


Asp Glu Asp Ala Arg Asn Ala Leu Ala Phe Ser Arg Phe Val Leu Gly 
        275                 280                 285             


Gly 
    


<210>  5
<211>  289
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mutant D-psicose 3-epimerase

<400>  5

Met Lys His Gly Ile Tyr Tyr Ala Tyr Trp Glu Gln Glu Trp Glu Ala 
1               5                   10                  15      


Lys Phe Gly Pro Tyr Ile Glu Lys Val Ala Lys Leu Gly Phe Asp Ile 
            20                  25                  30          


Leu Glu Val Ala Ala His His Ile Asn Glu Tyr Ser Asp Ala Glu Leu 
        35                  40                  45              


Ala Thr Ile Arg Lys Ser Ala Lys Asp Asn Gly Ile Ile Leu Thr Ala 
    50                  55                  60                  


Gly Ile Gly Pro Ser Lys Thr Lys Asn Leu Ser Ser Glu Asp Ala Ala 
65                  70                  75                  80  


Val Arg Ala Ala Gly Lys Ala Phe Phe Glu Arg Thr Leu Ser Asn Val 
                85                  90                  95      


Ala Lys Leu Asp Ile His Thr Ile Gly Gly Ala Leu His Ser Tyr Trp 
            100                 105                 110         


Pro Ile Asp Tyr Ser Gln Pro Val Asp Lys Ala Gly Asp Tyr Ala Arg 
        115                 120                 125             


Gly Val Glu Gly Ile Asn Gly Ile Ala Asp Phe Ala Asn Asp Leu Gly 
    130                 135                 140                 


Ile Asn Leu Cys Ile Glu Val Leu Asn Arg Phe Glu Asn His Val Leu 
145                 150                 155                 160 


Asn Thr Ala Ala Glu Gly Val Ala Phe Val Lys Asp Val Gly Lys Asn 
                165                 170                 175     


Asn Val Lys Val Met Leu Asp Thr Phe His Met Asn Ile Glu Glu Asp 
            180                 185                 190         


Ser Phe Gly Asp Ala Ile Arg Thr Ala Gly Pro Leu Leu Gly His Phe 
        195                 200                 205             


His Thr Gly Glu Ser Asn Arg Arg Val Pro Gly Lys Gly Arg Met Pro 
    210                 215                 220                 


Trp His Glu Ile Gly Leu Ala Leu Arg Asp Ile Asn Tyr Thr Gly Ala 
225                 230                 235                 240 


Val Ile Met Glu Pro Phe Val Lys Thr Gly Gly Thr Ile Gly Ser Asp 
                245                 250                 255     


Ile Lys Val Trp Arg Asp Leu Ser Gly Gly Ala Asp Ile Ala Lys Met 
            260                 265                 270         


Asp Glu Asp Ala Arg Asn Ala Leu Ala Phe Ser Arg Phe Val Leu Gly 
        275                 280                 285             


Gly 
    


<210>  6
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for amplification of nucleotide sequence coding 
       for Ara A and D of the BAD operon

<400>  6
gcccgggaga tctatgacga tttttgataa ttatg                                  35


<210>  7
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for amplification of nucleotide sequence coding 
       for Ara A and D of the BAD operon

<400>  7
gcggaattct tactgcccgt aatatgcct                                         29


<210>  8
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for amplification of nucleotide sequence encoding 
       for D-psicose 3-epimerase gene expression cassette including Ara 
       BAD promoter and rrnB terminator

<400>  8
ccgggctagc gaagaaacca attgtccata ttgcatc                                37


<210>  9
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for amplification of nucleotide sequence encoding 
       for D-psicose 3-epimerase gene expression cassette including Ara 
       BAD promoter and rrnB terminator

<400>  9
ccgggtcgac agagtttgta gaaacgcaaa aaggcc                                 36


