                         SEQUENCE LISTING

<110>  Katholieke Universiteit Leuven
 
<120>  RECOMBINANT PROTEIN PRODUCTION SYSTEM

<130>  ZL916048

<150>  GB16220073.3
<151>  2016-12-23

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  645
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(645)
<223>  Human RPL10 cDNA sequence

<220>
<221>  CDS
<222>  (1)..(645)

<400>  1
atg ggc cgc cgc ccc gcc cgt tgt tac cgg tat tgt aag aac aag ccg         48
Met Gly Arg Arg Pro Ala Arg Cys Tyr Arg Tyr Cys Lys Asn Lys Pro           
1               5                   10                  15                

tac cca aag tct cgc ttc tgc cga ggt gtc cct gat gcc aag att cgc         96
Tyr Pro Lys Ser Arg Phe Cys Arg Gly Val Pro Asp Ala Lys Ile Arg           
            20                  25                  30                    

att ttt gac ctg ggg cgg aaa aag gca aaa gtg gat gag ttt ccg ctt        144
Ile Phe Asp Leu Gly Arg Lys Lys Ala Lys Val Asp Glu Phe Pro Leu           
        35                  40                  45                        

tgt ggc cac atg gtg tca gat gaa tat gag cag ctg tcc tct gaa gcc        192
Cys Gly His Met Val Ser Asp Glu Tyr Glu Gln Leu Ser Ser Glu Ala           
    50                  55                  60                            

ctg gag gct gcc cga att tgt gcc aat aag tac atg gta aaa agt tgt        240
Leu Glu Ala Ala Arg Ile Cys Ala Asn Lys Tyr Met Val Lys Ser Cys           
65                  70                  75                  80            

ggc aaa gat ggc ttc cat atc cgg gtg cgg ctc cac ccc ttc cac gtc        288
Gly Lys Asp Gly Phe His Ile Arg Val Arg Leu His Pro Phe His Val           
                85                  90                  95                

atc cgc atc aac aag atg ttg tcc tgt gct ggg gct gac agg ctc caa        336
Ile Arg Ile Asn Lys Met Leu Ser Cys Ala Gly Ala Asp Arg Leu Gln           
            100                 105                 110                   

aca ggc atg cga ggt gcc ttt gga aag ccc cag ggc act gtg gcc agg        384
Thr Gly Met Arg Gly Ala Phe Gly Lys Pro Gln Gly Thr Val Ala Arg           
        115                 120                 125                       

gtt cac att ggc caa gtt atc atg tcc atc cgc acc aag ctg cag aac        432
Val His Ile Gly Gln Val Ile Met Ser Ile Arg Thr Lys Leu Gln Asn           
    130                 135                 140                           

aag gag cat gtg att gag gcc ctg cgc agg gcc aag ttc aag ttt cct        480
Lys Glu His Val Ile Glu Ala Leu Arg Arg Ala Lys Phe Lys Phe Pro           
145                 150                 155                 160           

ggc cgc cag aag atc cac atc tca aag aag tgg ggc ttc acc aag ttc        528
Gly Arg Gln Lys Ile His Ile Ser Lys Lys Trp Gly Phe Thr Lys Phe           
                165                 170                 175               

aat gct gat gaa ttt gaa gac atg gtg gct gaa aag cgg ctc atc cca        576
Asn Ala Asp Glu Phe Glu Asp Met Val Ala Glu Lys Arg Leu Ile Pro           
            180                 185                 190                   

gat ggc tgt ggg gtc aag tac atc ccc agt cgt ggc cct ctg gac aag        624
Asp Gly Cys Gly Val Lys Tyr Ile Pro Ser Arg Gly Pro Leu Asp Lys           
        195                 200                 205                       

tgg cgg gcc ctg cac tca tga                                            645
Trp Arg Ala Leu His Ser                                                   
    210                                                                   


<210>  2
<211>  214
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Gly Arg Arg Pro Ala Arg Cys Tyr Arg Tyr Cys Lys Asn Lys Pro 
1               5                   10                  15      


Tyr Pro Lys Ser Arg Phe Cys Arg Gly Val Pro Asp Ala Lys Ile Arg 
            20                  25                  30          


Ile Phe Asp Leu Gly Arg Lys Lys Ala Lys Val Asp Glu Phe Pro Leu 
        35                  40                  45              


Cys Gly His Met Val Ser Asp Glu Tyr Glu Gln Leu Ser Ser Glu Ala 
    50                  55                  60                  


Leu Glu Ala Ala Arg Ile Cys Ala Asn Lys Tyr Met Val Lys Ser Cys 
65                  70                  75                  80  


Gly Lys Asp Gly Phe His Ile Arg Val Arg Leu His Pro Phe His Val 
                85                  90                  95      


Ile Arg Ile Asn Lys Met Leu Ser Cys Ala Gly Ala Asp Arg Leu Gln 
            100                 105                 110         


Thr Gly Met Arg Gly Ala Phe Gly Lys Pro Gln Gly Thr Val Ala Arg 
        115                 120                 125             


Val His Ile Gly Gln Val Ile Met Ser Ile Arg Thr Lys Leu Gln Asn 
    130                 135                 140                 


Lys Glu His Val Ile Glu Ala Leu Arg Arg Ala Lys Phe Lys Phe Pro 
145                 150                 155                 160 


Gly Arg Gln Lys Ile His Ile Ser Lys Lys Trp Gly Phe Thr Lys Phe 
                165                 170                 175     


Asn Ala Asp Glu Phe Glu Asp Met Val Ala Glu Lys Arg Leu Ile Pro 
            180                 185                 190         


Asp Gly Cys Gly Val Lys Tyr Ile Pro Ser Arg Gly Pro Leu Asp Lys 
        195                 200                 205             


Trp Arg Ala Leu His Ser 
    210                 


<210>  3
<211>  213
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(213)
<223>  mouse RPL10 protein sequence

<400>  3

Met Gly Arg Arg Pro Ala Arg Cys Tyr Arg Tyr Cys Lys Asn Lys Pro 
1               5                   10                  15      


Tyr Pro Lys Ser Arg Phe Cys Arg Gly Val Pro Asp Ala Lys Ile Arg 
            20                  25                  30          


Ile Phe Asp Leu Gly Arg Lys Lys Ala Lys Val Asp Glu Phe Pro Leu 
        35                  40                  45              


Cys Gly His Met Val Ser Asp Glu Tyr Glu Gln Leu Ser Ser Glu Ala 
    50                  55                  60                  


Leu Glu Ala Ala Arg Ile Cys Ala Asn Lys Tyr Met Val Lys Ser Cys 
65                  70                  75                  80  


Gly Lys Asp Gly Phe His Ile Arg Val Arg Leu His Pro Phe His Val 
                85                  90                  95      


Ile Arg Ile Asn Lys Met Leu Ser Cys Ala Gly Ala Asp Arg Leu Gln 
            100                 105                 110         


Thr Gly Met Arg Gly Ala Phe Gly Lys Pro Gln Gly Thr Val Ala Arg 
        115                 120                 125             


Val His Ile Gly Gln Val Ile Met Ser Ile Arg Thr Lys Leu Gln Asn 
    130                 135                 140                 


Lys Glu His Val Ile Glu Ala Leu Arg Arg Ala Lys Phe Lys Phe Pro 
145                 150                 155                 160 


Gly Arg Gln Lys Ile His Ile Ser Lys Lys Trp Gly Phe Thr Lys Phe 
                165                 170                 175     


Asn Ala Asp Glu Phe Glu Asp Met Val Ala Glu Lys Arg Leu Ile Pro 
            180                 185                 190         


Asp Gly Cys Gly Val Lys Tyr Ile Pro Asn Arg Gly Pro Leu Asp Lys 
        195                 200                 205             


Trp Ala Leu His Ser 
    210             


<210>  4
<211>  221
<212>  PRT
<213>  Saccharomyces cerevisiae


<220>
<221>  MISC_FEATURE
<222>  (1)..(221)
<223>  yeast RPL10 protein sequence

<400>  4

Met Ala Arg Arg Pro Ala Arg Cys Tyr Arg Tyr Gln Lys Asn Lys Pro 
1               5                   10                  15      


Tyr Pro Lys Ser Arg Tyr Asn Arg Ala Val Pro Asp Ser Lys Ile Arg 
            20                  25                  30          


Ile Tyr Asp Leu Gly Lys Lys Lys Ala Thr Val Asp Glu Phe Pro Leu 
        35                  40                  45              


Cys Val His Leu Val Ser Asn Glu Leu Glu Gln Leu Ser Ser Glu Ala 
    50                  55                  60                  


Leu Glu Ala Ala Arg Ile Cys Ala Asn Lys Tyr Met Thr Thr Val Ser 
65                  70                  75                  80  


Gly Arg Asp Ala Phe His Leu Arg Val Arg Val His Pro Phe His Val 
                85                  90                  95      


Leu Arg Ile Asn Lys Met Leu Ser Cys Ala Gly Ala Asp Arg Leu Gln 
            100                 105                 110         


Gln Gly Met Arg Gly Ala Trp Gly Lys Pro His Gly Leu Ala Ala Arg 
        115                 120                 125             


Val Asp Ile Gly Gln Ile Ile Phe Ser Val Arg Thr Lys Asp Ser Asn 
    130                 135                 140                 


Lys Asp Val Val Val Glu Gly Leu Arg Arg Ala Arg Tyr Lys Phe Pro 
145                 150                 155                 160 


Gly Gln Gln Lys Ile Ile Leu Ser Lys Lys Trp Gly Phe Thr Asn Leu 
                165                 170                 175     


Asp Arg Pro Glu Tyr Leu Lys Lys Arg Glu Ala Gly Glu Val Lys Asp 
            180                 185                 190         


Asp Gly Ala Phe Val Lys Phe Leu Ser Lys Lys Gly Ser Leu Glu Asn 
        195                 200                 205             


Asn Ile Arg Glu Phe Pro Glu Tyr Phe Ala Ala Gln Ala 
    210                 215                 220     


<210>  5
<211>  2772
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct comprising human intracellular NOTCH


<220>
<221>  misc_feature
<222>  (1)..(2772)
<223>  human Notch

<220>
<221>  CDS
<222>  (1)..(2772)

<400>  5
atg ccg ccg ctc ctg gcg ccc ctg ctc tgc ctg gcg ctg ctg ccc gcg         48
Met Pro Pro Leu Leu Ala Pro Leu Leu Cys Leu Ala Leu Leu Pro Ala           
1               5                   10                  15                

ctc gcc gca cga ggc ccg cga gga tcc atc gtc tac ctg gag att gac         96
Leu Ala Ala Arg Gly Pro Arg Gly Ser Ile Val Tyr Leu Glu Ile Asp           
            20                  25                  30                    

aac cgg cag tgt gtg cag gcc tcc tcg cag tgc ttc cag agt gcc acc        144
Asn Arg Gln Cys Val Gln Ala Ser Ser Gln Cys Phe Gln Ser Ala Thr           
        35                  40                  45                        

gac gtg gcc gca ttc ctg gga gcg ctc gcc tcg ctg ggc agc ctc aac        192
Asp Val Ala Ala Phe Leu Gly Ala Leu Ala Ser Leu Gly Ser Leu Asn           
    50                  55                  60                            

atc ccc tac aag atc gag gcc gtg cag agt gag acc gtg gag ccg ccc        240
Ile Pro Tyr Lys Ile Glu Ala Val Gln Ser Glu Thr Val Glu Pro Pro           
65                  70                  75                  80            

ccg ccg gcg cag ctg cac ttc atg tac gtg gcg cag gcc gtg gag ccg        288
Pro Pro Ala Gln Leu His Phe Met Tyr Val Ala Gln Ala Val Glu Pro           
                85                  90                  95                

ccc ccg ccg gcg cag ctg cac ttc atg tac gtg gcg gcg gcc gcc ttt        336
Pro Pro Pro Ala Gln Leu His Phe Met Tyr Val Ala Ala Ala Ala Phe           
            100                 105                 110                   

gtg ctt ctg ttc ttc gtg ggc tgc ggg gtg ctg ctg tcc cgc aag cgc        384
Val Leu Leu Phe Phe Val Gly Cys Gly Val Leu Leu Ser Arg Lys Arg           
        115                 120                 125                       

cgg cgg cag cat ggc cag ctc tgg ttc cct gag ggc ttc aaa gtg tct        432
Arg Arg Gln His Gly Gln Leu Trp Phe Pro Glu Gly Phe Lys Val Ser           
    130                 135                 140                           

gag gcc agc aag aag aag cgg cgg gag ccc ctc ggc gag gac tcc gtg        480
Glu Ala Ser Lys Lys Lys Arg Arg Glu Pro Leu Gly Glu Asp Ser Val           
145                 150                 155                 160           

ggc ctc aag ccc ctg aag aac gct tca gac ggt gcc ctc atg gac gac        528
Gly Leu Lys Pro Leu Lys Asn Ala Ser Asp Gly Ala Leu Met Asp Asp           
                165                 170                 175               

aac cag aat gag tgg ggg gac gag gac ctg gag acc aag aag ttc cgg        576
Asn Gln Asn Glu Trp Gly Asp Glu Asp Leu Glu Thr Lys Lys Phe Arg           
            180                 185                 190                   

ttc gag gag ccc gtg gtt ctg cct gac ctg gac gac cag aca gac cac        624
Phe Glu Glu Pro Val Val Leu Pro Asp Leu Asp Asp Gln Thr Asp His           
        195                 200                 205                       

cgg cag tgg act cag cag cac ctg gat gcc gct gac ctg cgc atg tct        672
Arg Gln Trp Thr Gln Gln His Leu Asp Ala Ala Asp Leu Arg Met Ser           
    210                 215                 220                           

gcc atg gcc ccc aca ccg ccc cag ggt gag gtt gac gcc gac tgc atg        720
Ala Met Ala Pro Thr Pro Pro Gln Gly Glu Val Asp Ala Asp Cys Met           
225                 230                 235                 240           

gac gtc aat gtc cgc ggg cct gat ggc ttc acc ccg ctc atg atc gcc        768
Asp Val Asn Val Arg Gly Pro Asp Gly Phe Thr Pro Leu Met Ile Ala           
                245                 250                 255               

tcc tgc agc ggg ggc ggc ctg gag acg ggc aac agc gag gaa gag gag        816
Ser Cys Ser Gly Gly Gly Leu Glu Thr Gly Asn Ser Glu Glu Glu Glu           
            260                 265                 270                   

gac gcg ccg gcc gtc atc tcc gac ttc atc tac cag ggc gcc agc ctg        864
Asp Ala Pro Ala Val Ile Ser Asp Phe Ile Tyr Gln Gly Ala Ser Leu           
        275                 280                 285                       

cac aac cag aca gac cgc acg ggc gag acc gcc ttg cac ctg gcc gcc        912
His Asn Gln Thr Asp Arg Thr Gly Glu Thr Ala Leu His Leu Ala Ala           
    290                 295                 300                           

cgc tac tca cgc tct gat gcc gcc aag cgc ctg ctg gag gcc agc gca        960
Arg Tyr Ser Arg Ser Asp Ala Ala Lys Arg Leu Leu Glu Ala Ser Ala           
305                 310                 315                 320           

gat gcc aac atc cag gac aac atg ggc cgc acc ccg ctg cat gcg gct       1008
Asp Ala Asn Ile Gln Asp Asn Met Gly Arg Thr Pro Leu His Ala Ala           
                325                 330                 335               

gtg tct gcc gac gca caa ggt gtc ttc cag atc ctg atc cgg aac cga       1056
Val Ser Ala Asp Ala Gln Gly Val Phe Gln Ile Leu Ile Arg Asn Arg           
            340                 345                 350                   

gcc aca gac ctg gat gcc cgc atg cat gat ggc acg acg cca ctg atc       1104
Ala Thr Asp Leu Asp Ala Arg Met His Asp Gly Thr Thr Pro Leu Ile           
        355                 360                 365                       

ctg gct gcc cgc ctg gcc gtg gag ggc atg ctg gag gac ctc atc aac       1152
Leu Ala Ala Arg Leu Ala Val Glu Gly Met Leu Glu Asp Leu Ile Asn           
    370                 375                 380                           

tca cac gcc gac gtc aac gcc gta gat gac ctg ggc aag tcc gcc ctg       1200
Ser His Ala Asp Val Asn Ala Val Asp Asp Leu Gly Lys Ser Ala Leu           
385                 390                 395                 400           

cac tgg gcc gcc gcc gtg aac aat gtg gat gcc gca gtt gtg ctc ctg       1248
His Trp Ala Ala Ala Val Asn Asn Val Asp Ala Ala Val Val Leu Leu           
                405                 410                 415               

aag aac ggg gct aac aaa gat atg cag aac aac agg gag gag aca ccc       1296
Lys Asn Gly Ala Asn Lys Asp Met Gln Asn Asn Arg Glu Glu Thr Pro           
            420                 425                 430                   

ctg ttt ctg gcc gcc cgg gag ggc agc tac gag acc gcc aag gtg ctg       1344
Leu Phe Leu Ala Ala Arg Glu Gly Ser Tyr Glu Thr Ala Lys Val Leu           
        435                 440                 445                       

ctg gac cac ttt gcc aac cgg gac atc acg gat cat atg gac cgc ctg       1392
Leu Asp His Phe Ala Asn Arg Asp Ile Thr Asp His Met Asp Arg Leu           
    450                 455                 460                           

ccg cgc gac atc gca cag gag cgc atg cat cac gac atc gtg agg ctg       1440
Pro Arg Asp Ile Ala Gln Glu Arg Met His His Asp Ile Val Arg Leu           
465                 470                 475                 480           

ctg gac gag tac aac ctg gtg cgc agc ccg cag ctg cac gga gcc ccg       1488
Leu Asp Glu Tyr Asn Leu Val Arg Ser Pro Gln Leu His Gly Ala Pro           
                485                 490                 495               

ctg ggg ggc acg ccc acc ctg tcg ccc ccg ctc tgc tcg ccc aac ggc       1536
Leu Gly Gly Thr Pro Thr Leu Ser Pro Pro Leu Cys Ser Pro Asn Gly           
            500                 505                 510                   

tac ctg ggc agc ctc aag ccc ggc gtg cag ggc aag aag gtc cgc aag       1584
Tyr Leu Gly Ser Leu Lys Pro Gly Val Gln Gly Lys Lys Val Arg Lys           
        515                 520                 525                       

ccc agc agc aaa ggc ctg gcc tgt gga agc aag gag gcc aag gac ctc       1632
Pro Ser Ser Lys Gly Leu Ala Cys Gly Ser Lys Glu Ala Lys Asp Leu           
    530                 535                 540                           

aag gca cgg agg aag aag tcc cag gac ggc aag ggc tgc ctg ctg gac       1680
Lys Ala Arg Arg Lys Lys Ser Gln Asp Gly Lys Gly Cys Leu Leu Asp           
545                 550                 555                 560           

agc tcc ggc atg ctc tcg ccc gtg gac tcc ctg gag tca ccc cat ggc       1728
Ser Ser Gly Met Leu Ser Pro Val Asp Ser Leu Glu Ser Pro His Gly           
                565                 570                 575               

tac ctg tca gac gtg gcc tcg ccg cca ctg ctg ccc tcc ccg ttc cag       1776
Tyr Leu Ser Asp Val Ala Ser Pro Pro Leu Leu Pro Ser Pro Phe Gln           
            580                 585                 590                   

cag tct ccg tcc gtg ccc ctc aac cac ctg cct ggg atg ccc gac acc       1824
Gln Ser Pro Ser Val Pro Leu Asn His Leu Pro Gly Met Pro Asp Thr           
        595                 600                 605                       

cac ctg ggc atc ggg cac ctg aac gtg gcg gcc aag ccc gag atg gcg       1872
His Leu Gly Ile Gly His Leu Asn Val Ala Ala Lys Pro Glu Met Ala           
    610                 615                 620                           

gcg ctg ggt ggg ggc ggc cgg ctg gcc ttt gag act ggc cca cct cgt       1920
Ala Leu Gly Gly Gly Gly Arg Leu Ala Phe Glu Thr Gly Pro Pro Arg           
625                 630                 635                 640           

ctc tcc cac ctg cct gtg gcc tct ggc acc agc acc gtc ctg ggc tcc       1968
Leu Ser His Leu Pro Val Ala Ser Gly Thr Ser Thr Val Leu Gly Ser           
                645                 650                 655               

agc agc gga ggg gcc ctg aat ttc act gtg ggc ggg tcc acc agt ttg       2016
Ser Ser Gly Gly Ala Leu Asn Phe Thr Val Gly Gly Ser Thr Ser Leu           
            660                 665                 670                   

aat ggt caa tgc gag tgg ctg tcc cgg ctg cag agc ggc atg gtg ccg       2064
Asn Gly Gln Cys Glu Trp Leu Ser Arg Leu Gln Ser Gly Met Val Pro           
        675                 680                 685                       

aac caa tac aac cct ctg cgg ggg agt gtg gca cca ggc ccc ctg agc       2112
Asn Gln Tyr Asn Pro Leu Arg Gly Ser Val Ala Pro Gly Pro Leu Ser           
    690                 695                 700                           

aca cag gcc ccc tcc ctg cag cat ggc atg gta ggc ccg ctg cac agt       2160
Thr Gln Ala Pro Ser Leu Gln His Gly Met Val Gly Pro Leu His Ser           
705                 710                 715                 720           

agc ctt gct gcc agc gcc ctg tcc cag atg atg agc tac cag ggc ctg       2208
Ser Leu Ala Ala Ser Ala Leu Ser Gln Met Met Ser Tyr Gln Gly Leu           
                725                 730                 735               

ccc agc acc cgg ctg gcc acc cag cct cac ctg gtg cag acc cag cag       2256
Pro Ser Thr Arg Leu Ala Thr Gln Pro His Leu Val Gln Thr Gln Gln           
            740                 745                 750                   

gtg cag cca caa aac tta cag atg cag cag cag aac ctg cag cca gca       2304
Val Gln Pro Gln Asn Leu Gln Met Gln Gln Gln Asn Leu Gln Pro Ala           
        755                 760                 765                       

aac atc cag cag cag caa agc ctg cag ccg cca cca cca cca cca cag       2352
Asn Ile Gln Gln Gln Gln Ser Leu Gln Pro Pro Pro Pro Pro Pro Gln           
    770                 775                 780                           

ccg cac ctt ggc gtg agc tca gca gcc agc ggc cac ctg ggc cgg agc       2400
Pro His Leu Gly Val Ser Ser Ala Ala Ser Gly His Leu Gly Arg Ser           
785                 790                 795                 800           

ttc ctg agt gga gag ccg agc cag gca gac gtg cag cca ctg ggc ccc       2448
Phe Leu Ser Gly Glu Pro Ser Gln Ala Asp Val Gln Pro Leu Gly Pro           
                805                 810                 815               

agc agc ctg gcg gtg cac act att ctg ccc cag gag agc ccc gcc ctg       2496
Ser Ser Leu Ala Val His Thr Ile Leu Pro Gln Glu Ser Pro Ala Leu           
            820                 825                 830                   

ccc acg tcg ctg cca tcc tcg ctg gtc cca ccc gtg acc gca gcc cag       2544
Pro Thr Ser Leu Pro Ser Ser Leu Val Pro Pro Val Thr Ala Ala Gln           
        835                 840                 845                       

ttc ctg acg ccc ccc tcg cag cac ags tac tcc tyg cct gtg gac aac       2592
Phe Leu Thr Pro Pro Ser Gln His Xaa Tyr Ser Xaa Pro Val Asp Asn           
    850                 855                 860                           

acc ccc agc cac cag cta cag gtg cct gag cac ccc ttc ctc acc ccg       2640
Thr Pro Ser His Gln Leu Gln Val Pro Glu His Pro Phe Leu Thr Pro           
865                 870                 875                 880           

tcc cct gag tcc cct gac cag tgg tcc agc tyg tcc ccg cat tcc aac       2688
Ser Pro Glu Ser Pro Asp Gln Trp Ser Ser Xaa Ser Pro His Ser Asn           
                885                 890                 895               

gtc tcc gac tgg tcc gag ggc gtc tcc agc cct ccc acc agc atg cag       2736
Val Ser Asp Trp Ser Glu Gly Val Ser Ser Pro Pro Thr Ser Met Gln           
            900                 905                 910                   

tcc cag atc gcc cgc att ccg gag gcc ttc aag taa                       2772
Ser Gln Ile Ala Arg Ile Pro Glu Ala Phe Lys                               
        915                 920                                           


<210>  6
<211>  923
<212>  PRT
<213>  Artificial Sequence

<220>
<221>  misc_feature
<222>  (857)..(857)
<223>  The 'Xaa' at location 857 stands for Arg, or Ser.

<220>
<221>  misc_feature
<222>  (860)..(860)
<223>  The 'Xaa' at location 860 stands for Ser, or Leu.

<220>
<221>  misc_feature
<222>  (891)..(891)
<223>  The 'Xaa' at location 891 stands for Ser, or Leu.

<220>
<223>  Synthetic Construct

<400>  6

Met Pro Pro Leu Leu Ala Pro Leu Leu Cys Leu Ala Leu Leu Pro Ala 
1               5                   10                  15      


Leu Ala Ala Arg Gly Pro Arg Gly Ser Ile Val Tyr Leu Glu Ile Asp 
            20                  25                  30          


Asn Arg Gln Cys Val Gln Ala Ser Ser Gln Cys Phe Gln Ser Ala Thr 
        35                  40                  45              


Asp Val Ala Ala Phe Leu Gly Ala Leu Ala Ser Leu Gly Ser Leu Asn 
    50                  55                  60                  


Ile Pro Tyr Lys Ile Glu Ala Val Gln Ser Glu Thr Val Glu Pro Pro 
65                  70                  75                  80  


Pro Pro Ala Gln Leu His Phe Met Tyr Val Ala Gln Ala Val Glu Pro 
                85                  90                  95      


Pro Pro Pro Ala Gln Leu His Phe Met Tyr Val Ala Ala Ala Ala Phe 
            100                 105                 110         


Val Leu Leu Phe Phe Val Gly Cys Gly Val Leu Leu Ser Arg Lys Arg 
        115                 120                 125             


Arg Arg Gln His Gly Gln Leu Trp Phe Pro Glu Gly Phe Lys Val Ser 
    130                 135                 140                 


Glu Ala Ser Lys Lys Lys Arg Arg Glu Pro Leu Gly Glu Asp Ser Val 
145                 150                 155                 160 


Gly Leu Lys Pro Leu Lys Asn Ala Ser Asp Gly Ala Leu Met Asp Asp 
                165                 170                 175     


Asn Gln Asn Glu Trp Gly Asp Glu Asp Leu Glu Thr Lys Lys Phe Arg 
            180                 185                 190         


Phe Glu Glu Pro Val Val Leu Pro Asp Leu Asp Asp Gln Thr Asp His 
        195                 200                 205             


Arg Gln Trp Thr Gln Gln His Leu Asp Ala Ala Asp Leu Arg Met Ser 
    210                 215                 220                 


Ala Met Ala Pro Thr Pro Pro Gln Gly Glu Val Asp Ala Asp Cys Met 
225                 230                 235                 240 


Asp Val Asn Val Arg Gly Pro Asp Gly Phe Thr Pro Leu Met Ile Ala 
                245                 250                 255     


Ser Cys Ser Gly Gly Gly Leu Glu Thr Gly Asn Ser Glu Glu Glu Glu 
            260                 265                 270         


Asp Ala Pro Ala Val Ile Ser Asp Phe Ile Tyr Gln Gly Ala Ser Leu 
        275                 280                 285             


His Asn Gln Thr Asp Arg Thr Gly Glu Thr Ala Leu His Leu Ala Ala 
    290                 295                 300                 


Arg Tyr Ser Arg Ser Asp Ala Ala Lys Arg Leu Leu Glu Ala Ser Ala 
305                 310                 315                 320 


Asp Ala Asn Ile Gln Asp Asn Met Gly Arg Thr Pro Leu His Ala Ala 
                325                 330                 335     


Val Ser Ala Asp Ala Gln Gly Val Phe Gln Ile Leu Ile Arg Asn Arg 
            340                 345                 350         


Ala Thr Asp Leu Asp Ala Arg Met His Asp Gly Thr Thr Pro Leu Ile 
        355                 360                 365             


Leu Ala Ala Arg Leu Ala Val Glu Gly Met Leu Glu Asp Leu Ile Asn 
    370                 375                 380                 


Ser His Ala Asp Val Asn Ala Val Asp Asp Leu Gly Lys Ser Ala Leu 
385                 390                 395                 400 


His Trp Ala Ala Ala Val Asn Asn Val Asp Ala Ala Val Val Leu Leu 
                405                 410                 415     


Lys Asn Gly Ala Asn Lys Asp Met Gln Asn Asn Arg Glu Glu Thr Pro 
            420                 425                 430         


Leu Phe Leu Ala Ala Arg Glu Gly Ser Tyr Glu Thr Ala Lys Val Leu 
        435                 440                 445             


Leu Asp His Phe Ala Asn Arg Asp Ile Thr Asp His Met Asp Arg Leu 
    450                 455                 460                 


Pro Arg Asp Ile Ala Gln Glu Arg Met His His Asp Ile Val Arg Leu 
465                 470                 475                 480 


Leu Asp Glu Tyr Asn Leu Val Arg Ser Pro Gln Leu His Gly Ala Pro 
                485                 490                 495     


Leu Gly Gly Thr Pro Thr Leu Ser Pro Pro Leu Cys Ser Pro Asn Gly 
            500                 505                 510         


Tyr Leu Gly Ser Leu Lys Pro Gly Val Gln Gly Lys Lys Val Arg Lys 
        515                 520                 525             


Pro Ser Ser Lys Gly Leu Ala Cys Gly Ser Lys Glu Ala Lys Asp Leu 
    530                 535                 540                 


Lys Ala Arg Arg Lys Lys Ser Gln Asp Gly Lys Gly Cys Leu Leu Asp 
545                 550                 555                 560 


Ser Ser Gly Met Leu Ser Pro Val Asp Ser Leu Glu Ser Pro His Gly 
                565                 570                 575     


Tyr Leu Ser Asp Val Ala Ser Pro Pro Leu Leu Pro Ser Pro Phe Gln 
            580                 585                 590         


Gln Ser Pro Ser Val Pro Leu Asn His Leu Pro Gly Met Pro Asp Thr 
        595                 600                 605             


His Leu Gly Ile Gly His Leu Asn Val Ala Ala Lys Pro Glu Met Ala 
    610                 615                 620                 


Ala Leu Gly Gly Gly Gly Arg Leu Ala Phe Glu Thr Gly Pro Pro Arg 
625                 630                 635                 640 


Leu Ser His Leu Pro Val Ala Ser Gly Thr Ser Thr Val Leu Gly Ser 
                645                 650                 655     


Ser Ser Gly Gly Ala Leu Asn Phe Thr Val Gly Gly Ser Thr Ser Leu 
            660                 665                 670         


Asn Gly Gln Cys Glu Trp Leu Ser Arg Leu Gln Ser Gly Met Val Pro 
        675                 680                 685             


Asn Gln Tyr Asn Pro Leu Arg Gly Ser Val Ala Pro Gly Pro Leu Ser 
    690                 695                 700                 


Thr Gln Ala Pro Ser Leu Gln His Gly Met Val Gly Pro Leu His Ser 
705                 710                 715                 720 


Ser Leu Ala Ala Ser Ala Leu Ser Gln Met Met Ser Tyr Gln Gly Leu 
                725                 730                 735     


Pro Ser Thr Arg Leu Ala Thr Gln Pro His Leu Val Gln Thr Gln Gln 
            740                 745                 750         


Val Gln Pro Gln Asn Leu Gln Met Gln Gln Gln Asn Leu Gln Pro Ala 
        755                 760                 765             


Asn Ile Gln Gln Gln Gln Ser Leu Gln Pro Pro Pro Pro Pro Pro Gln 
    770                 775                 780                 


Pro His Leu Gly Val Ser Ser Ala Ala Ser Gly His Leu Gly Arg Ser 
785                 790                 795                 800 


Phe Leu Ser Gly Glu Pro Ser Gln Ala Asp Val Gln Pro Leu Gly Pro 
                805                 810                 815     


Ser Ser Leu Ala Val His Thr Ile Leu Pro Gln Glu Ser Pro Ala Leu 
            820                 825                 830         


Pro Thr Ser Leu Pro Ser Ser Leu Val Pro Pro Val Thr Ala Ala Gln 
        835                 840                 845             


Phe Leu Thr Pro Pro Ser Gln His Xaa Tyr Ser Xaa Pro Val Asp Asn 
    850                 855                 860                 


Thr Pro Ser His Gln Leu Gln Val Pro Glu His Pro Phe Leu Thr Pro 
865                 870                 875                 880 


Ser Pro Glu Ser Pro Asp Gln Trp Ser Ser Xaa Ser Pro His Ser Asn 
                885                 890                 895     


Val Ser Asp Trp Ser Glu Gly Val Ser Ser Pro Pro Thr Ser Met Gln 
            900                 905                 910         


Ser Gln Ile Ala Arg Ile Pro Glu Ala Phe Lys 
        915                 920             


<210>  7
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sh oligonucleotide for mouse RPL10

<400>  7
aaccgacgat cctattgtca tc                                                22


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RPL10 targeting gRNA

<400>  8
tcttgttgat gcggatgacg                                                   20


<210>  9
<211>  130
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA-Cas9 complex

<400>  9
cctgtcagcc ccagcacagg acaacatctt gttaatgctg atcacgtgaa aggggtggag       60

ccgcacccgg atatggaagc catctttgcc acaacttttt accatgtact tattggcaca      120

aattcgggca                                                             130


<210>  10
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RPL10 WT specific forward primer

<400>  10
cttccacgtc atccgcatc                                                    19


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RPL10 R98S specific forward primer

<400>  11
cctttcacgt gatcagcatt                                                   20


<210>  12
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RPL10 reverse primer

<400>  12
gctctgataa aataatgcaa gccta                                             25


