               SEQUENCE LISTING

<110> INSERM
      Université Paris Descartes

<120> Antidotes to anti-coagulant drugs allowing diagnosis and treatment

<130> PR76626

<160> 6

<170> BiSSAP 1.3

<210> 1
<211> 139
<212> PRT
<213> Homo sapiens

<220> 
<223> FX light chain

<400> 1
Ala Asn Ser Phe Leu Glu Glu Met Lys Lys Gly His Leu Glu Arg Glu 
1               5                   10                  15      
Cys Met Glu Glu Thr Cys Ser Tyr Glu Glu Ala Arg Glu Val Phe Glu 
            20                  25                  30          
Asp Ser Asp Lys Thr Asn Glu Phe Trp Asn Lys Tyr Lys Asp Gly Asp 
        35                  40                  45              
Gln Cys Glu Thr Ser Pro Cys Gln Asn Gln Gly Lys Cys Lys Asp Gly 
    50                  55                  60                  
Leu Gly Glu Tyr Thr Cys Thr Cys Leu Glu Gly Phe Glu Gly Lys Asn 
65                  70                  75                  80  
Cys Glu Leu Phe Thr Arg Lys Leu Cys Ser Leu Asp Asn Gly Asp Cys 
                85                  90                  95      
Asp Gln Phe Cys His Glu Glu Gln Asn Ser Val Val Cys Ser Cys Ala 
            100                 105                 110         
Arg Gly Tyr Thr Leu Ala Asp Asn Gly Lys Ala Cys Ile Pro Thr Gly 
        115                 120                 125             
Pro Tyr Pro Cys Gly Lys Gln Thr Leu Glu Arg 
    130                 135                 

<210> 2
<211> 306
<212> PRT
<213> Homo sapiens

<220> 
<223> FX heavy chain

<400> 2
Ser Val Ala Gln Ala Thr Ser Ser Ser Gly Glu Ala Pro Asp Ser Ile 
1               5                   10                  15      
Thr Trp Lys Pro Tyr Asp Ala Ala Asp Leu Asp Pro Thr Glu Asn Pro 
            20                  25                  30          
Phe Asp Leu Leu Asp Phe Asn Gln Thr Gln Pro Glu Arg Gly Asp Asn 
        35                  40                  45              
Asn Leu Thr Arg Ile Val Gly Gly Gln Glu Cys Lys Asp Gly Glu Cys 
    50                  55                  60                  
Pro Trp Gln Ala Leu Leu Ile Asn Glu Glu Asn Glu Gly Phe Cys Gly 
65                  70                  75                  80  
Gly Thr Ile Leu Ser Glu Phe Tyr Ile Leu Thr Ala Ala His Cys Leu 
                85                  90                  95      
Tyr Gln Ala Lys Arg Phe Lys Val Arg Val Gly Asp Arg Asn Thr Glu 
            100                 105                 110         
Gln Glu Glu Gly Gly Glu Ala Val His Glu Val Glu Val Val Ile Lys 
        115                 120                 125             
His Asn Arg Phe Thr Lys Glu Thr Tyr Asp Phe Asp Ile Ala Val Leu 
    130                 135                 140                 
Arg Leu Lys Thr Pro Ile Thr Phe Arg Met Asn Val Ala Pro Ala Cys 
145                 150                 155                 160 
Leu Pro Glu Arg Asp Trp Ala Glu Ser Thr Leu Met Thr Gln Lys Thr 
                165                 170                 175     
Gly Ile Val Ser Gly Phe Gly Arg Thr His Glu Lys Gly Arg Gln Ser 
            180                 185                 190         
Thr Arg Leu Lys Met Leu Glu Val Pro Tyr Val Asp Arg Asn Ser Cys 
        195                 200                 205             
Lys Leu Ser Ser Ser Phe Ile Ile Thr Gln Asn Met Phe Cys Ala Gly 
    210                 215                 220                 
Tyr Asp Thr Lys Gln Glu Asp Ala Cys Gln Gly Asp Ser Gly Gly Pro 
225                 230                 235                 240 
His Val Thr Arg Phe Lys Asp Thr Tyr Phe Val Thr Gly Ile Val Ser 
                245                 250                 255     
Trp Gly Glu Gly Cys Ala Arg Lys Gly Lys Tyr Gly Ile Tyr Thr Lys 
            260                 265                 270         
Val Thr Ala Phe Leu Lys Trp Ile Asp Arg Ser Met Lys Thr Arg Gly 
        275                 280                 285             
Leu Pro Lys Ala Lys Ser His Ala Pro Glu Val Ile Thr Ser Ser Pro 
    290                 295                 300                 
Leu Lys 
305     

<210> 3
<211> 52
<212> PRT
<213> Homo sapiens

<220> 
<223> N-terminal part of FX heavy chain

<400> 3
Ser Val Ala Gln Ala Thr Ser Ser Ser Gly Glu Ala Pro Asp Ser Ile 
1               5                   10                  15      
Thr Trp Lys Pro Tyr Asp Ala Ala Asp Leu Asp Pro Thr Glu Asn Pro 
            20                  25                  30          
Phe Asp Leu Leu Asp Phe Asn Gln Thr Gln Pro Glu Arg Gly Asp Asn 
        35                  40                  45              
Asn Leu Thr Arg 
    50          

<210> 4
<211> 254
<212> PRT
<213> Homo sapiens

<220> 
<223> FXa heavy chain

<400> 4
Ile Val Gly Gly Gln Glu Cys Lys Asp Gly Glu Cys Pro Trp Gln Ala 
1               5                   10                  15      
Leu Leu Ile Asn Glu Glu Asn Glu Gly Phe Cys Gly Gly Thr Ile Leu 
            20                  25                  30          
Ser Glu Phe Tyr Ile Leu Thr Ala Ala His Cys Leu Tyr Gln Ala Lys 
        35                  40                  45              
Arg Phe Lys Val Arg Val Gly Asp Arg Asn Thr Glu Gln Glu Glu Gly 
    50                  55                  60                  
Gly Glu Ala Val His Glu Val Glu Val Val Ile Lys His Asn Arg Phe 
65                  70                  75                  80  
Thr Lys Glu Thr Tyr Asp Phe Asp Ile Ala Val Leu Arg Leu Lys Thr 
                85                  90                  95      
Pro Ile Thr Phe Arg Met Asn Val Ala Pro Ala Cys Leu Pro Glu Arg 
            100                 105                 110         
Asp Trp Ala Glu Ser Thr Leu Met Thr Gln Lys Thr Gly Ile Val Ser 
        115                 120                 125             
Gly Phe Gly Arg Thr His Glu Lys Gly Arg Gln Ser Thr Arg Leu Lys 
    130                 135                 140                 
Met Leu Glu Val Pro Tyr Val Asp Arg Asn Ser Cys Lys Leu Ser Ser 
145                 150                 155                 160 
Ser Phe Ile Ile Thr Gln Asn Met Phe Cys Ala Gly Tyr Asp Thr Lys 
                165                 170                 175     
Gln Glu Asp Ala Cys Gln Gly Asp Ser Gly Gly Pro His Val Thr Arg 
            180                 185                 190         
Phe Lys Asp Thr Tyr Phe Val Thr Gly Ile Val Ser Trp Gly Glu Gly 
        195                 200                 205             
Cys Ala Arg Lys Gly Lys Tyr Gly Ile Tyr Thr Lys Val Thr Ala Phe 
    210                 215                 220                 
Leu Lys Trp Ile Asp Arg Ser Met Lys Thr Arg Gly Leu Pro Lys Ala 
225                 230                 235                 240 
Lys Ser His Ala Pro Glu Val Ile Thr Ser Ser Pro Leu Lys 
                245                 250                 

<210> 5
<211> 95
<212> PRT
<213> Homo sapiens

<220> 
<223> Light chain of Gla domain less FXa (GDFXa) resulting from
      chymotrypsin digestion

<400> 5
Lys Asp Gly Asp Gln Cys Glu Thr Ser Pro Cys Gln Asn Gln Gly Lys 
1               5                   10                  15      
Cys Lys Asp Gly Leu Gly Glu Tyr Thr Cys Thr Cys Leu Glu Gly Phe 
            20                  25                  30          
Glu Gly Lys Asn Cys Glu Leu Phe Thr Arg Lys Leu Cys Ser Leu Asp 
        35                  40                  45              
Asn Gly Asp Cys Asp Gln Phe Cys His Glu Glu Gln Asn Ser Val Val 
    50                  55                  60                  
Cys Ser Cys Ala Arg Gly Tyr Thr Leu Ala Asp Asn Gly Lys Ala Cys 
65                  70                  75                  80  
Ile Pro Thr Gly Pro Tyr Pro Cys Gly Lys Gln Thr Leu Glu Arg 
                85                  90                  95  

<210> 6
<211> 1474
<212> PRT
<213> Homo sapiens

<220> 
<223> Precursor Protein of human α2M

<400> 6
Met Gly Lys Asn Lys Leu Leu His Pro Ser Leu Val Leu Leu Leu Leu 
1               5                   10                  15      
Val Leu Leu Pro Thr Asp Ala Ser Val Ser Gly Lys Pro Gln Tyr Met 
            20                  25                  30          
Val Leu Val Pro Ser Leu Leu His Thr Glu Thr Thr Glu Lys Gly Cys 
        35                  40                  45              
Val Leu Leu Ser Tyr Leu Asn Glu Thr Val Thr Val Ser Ala Ser Leu 
    50                  55                  60                  
Glu Ser Val Arg Gly Asn Arg Ser Leu Phe Thr Asp Leu Glu Ala Glu 
65                  70                  75                  80  
Asn Asp Val Leu His Cys Val Ala Phe Ala Val Pro Lys Ser Ser Ser 
                85                  90                  95      
Asn Glu Glu Val Met Phe Leu Thr Val Gln Val Lys Gly Pro Thr Gln 
            100                 105                 110         
Glu Phe Lys Lys Arg Thr Thr Val Met Val Lys Asn Glu Asp Ser Leu 
        115                 120                 125             
Val Phe Val Gln Thr Asp Lys Ser Ile Tyr Lys Pro Gly Gln Thr Val 
    130                 135                 140                 
Lys Phe Arg Val Val Ser Met Asp Glu Asn Phe His Pro Leu Asn Glu 
145                 150                 155                 160 
Leu Ile Pro Leu Val Tyr Ile Gln Asp Pro Lys Gly Asn Arg Ile Ala 
                165                 170                 175     
Gln Trp Gln Ser Phe Gln Leu Glu Gly Gly Leu Lys Gln Phe Ser Phe 
            180                 185                 190         
Pro Leu Ser Ser Glu Pro Phe Gln Gly Ser Tyr Lys Val Val Val Gln 
        195                 200                 205             
Lys Lys Ser Gly Gly Arg Thr Glu His Pro Phe Thr Val Glu Glu Phe 
    210                 215                 220                 
Val Leu Pro Lys Phe Glu Val Gln Val Thr Val Pro Lys Ile Ile Thr 
225                 230                 235                 240 
Ile Leu Glu Glu Glu Met Asn Val Ser Val Cys Gly Leu Tyr Thr Tyr 
                245                 250                 255     
Gly Lys Pro Val Pro Gly His Val Thr Val Ser Ile Cys Arg Lys Tyr 
            260                 265                 270         
Ser Asp Ala Ser Asp Cys His Gly Glu Asp Ser Gln Ala Phe Cys Glu 
        275                 280                 285             
Lys Phe Ser Gly Gln Leu Asn Ser His Gly Cys Phe Tyr Gln Gln Val 
    290                 295                 300                 
Lys Thr Lys Val Phe Gln Leu Lys Arg Lys Glu Tyr Glu Met Lys Leu 
305                 310                 315                 320 
His Thr Glu Ala Gln Ile Gln Glu Glu Gly Thr Val Val Glu Leu Thr 
                325                 330                 335     
Gly Arg Gln Ser Ser Glu Ile Thr Arg Thr Ile Thr Lys Leu Ser Phe 
            340                 345                 350         
Val Lys Val Asp Ser His Phe Arg Gln Gly Ile Pro Phe Phe Gly Gln 
        355                 360                 365             
Val Arg Leu Val Asp Gly Lys Gly Val Pro Ile Pro Asn Lys Val Ile 
    370                 375                 380                 
Phe Ile Arg Gly Asn Glu Ala Asn Tyr Tyr Ser Asn Ala Thr Thr Asp 
385                 390                 395                 400 
Glu His Gly Leu Val Gln Phe Ser Ile Asn Thr Thr Asn Val Met Gly 
                405                 410                 415     
Thr Ser Leu Thr Val Arg Val Asn Tyr Lys Asp Arg Ser Pro Cys Tyr 
            420                 425                 430         
Gly Tyr Gln Trp Val Ser Glu Glu His Glu Glu Ala His His Thr Ala 
        435                 440                 445             
Tyr Leu Val Phe Ser Pro Ser Lys Ser Phe Val His Leu Glu Pro Met 
    450                 455                 460                 
Ser His Glu Leu Pro Cys Gly His Thr Gln Thr Val Gln Ala His Tyr 
465                 470                 475                 480 
Ile Leu Asn Gly Gly Thr Leu Leu Gly Leu Lys Lys Leu Ser Phe Tyr 
                485                 490                 495     
Tyr Leu Ile Met Ala Lys Gly Gly Ile Val Arg Thr Gly Thr His Gly 
            500                 505                 510         
Leu Leu Val Lys Gln Glu Asp Met Lys Gly His Phe Ser Ile Ser Ile 
        515                 520                 525             
Pro Val Lys Ser Asp Ile Ala Pro Val Ala Arg Leu Leu Ile Tyr Ala 
    530                 535                 540                 
Val Leu Pro Thr Gly Asp Val Ile Gly Asp Ser Ala Lys Tyr Asp Val 
545                 550                 555                 560 
Glu Asn Cys Leu Ala Asn Lys Val Asp Leu Ser Phe Ser Pro Ser Gln 
                565                 570                 575     
Ser Leu Pro Ala Ser His Ala His Leu Arg Val Thr Ala Ala Pro Gln 
            580                 585                 590         
Ser Val Cys Ala Leu Arg Ala Val Asp Gln Ser Val Leu Leu Met Lys 
        595                 600                 605             
Pro Asp Ala Glu Leu Ser Ala Ser Ser Val Tyr Asn Leu Leu Pro Glu 
    610                 615                 620                 
Lys Asp Leu Thr Gly Phe Pro Gly Pro Leu Asn Asp Gln Asp Asn Glu 
625                 630                 635                 640 
Asp Cys Ile Asn Arg His Asn Val Tyr Ile Asn Gly Ile Thr Tyr Thr 
                645                 650                 655     
Pro Val Ser Ser Thr Asn Glu Lys Asp Met Tyr Ser Phe Leu Glu Asp 
            660                 665                 670         
Met Gly Leu Lys Ala Phe Thr Asn Ser Lys Ile Arg Lys Pro Lys Met 
        675                 680                 685             
Cys Pro Gln Leu Gln Gln Tyr Glu Met His Gly Pro Glu Gly Leu Arg 
    690                 695                 700                 
Val Gly Phe Tyr Glu Ser Asp Val Met Gly Arg Gly His Ala Arg Leu 
705                 710                 715                 720 
Val His Val Glu Glu Pro His Thr Glu Thr Val Arg Lys Tyr Phe Pro 
                725                 730                 735     
Glu Thr Trp Ile Trp Asp Leu Val Val Val Asn Ser Ala Gly Val Ala 
            740                 745                 750         
Glu Val Gly Val Thr Val Pro Asp Thr Ile Thr Glu Trp Lys Ala Gly 
        755                 760                 765             
Ala Phe Cys Leu Ser Glu Asp Ala Gly Leu Gly Ile Ser Ser Thr Ala 
    770                 775                 780                 
Ser Leu Arg Ala Phe Gln Pro Phe Phe Val Glu Leu Thr Met Pro Tyr 
785                 790                 795                 800 
Ser Val Ile Arg Gly Glu Ala Phe Thr Leu Lys Ala Thr Val Leu Asn 
                805                 810                 815     
Tyr Leu Pro Lys Cys Ile Arg Val Ser Val Gln Leu Glu Ala Ser Pro 
            820                 825                 830         
Ala Phe Leu Ala Val Pro Val Glu Lys Glu Gln Ala Pro His Cys Ile 
        835                 840                 845             
Cys Ala Asn Gly Arg Gln Thr Val Ser Trp Ala Val Thr Pro Lys Ser 
    850                 855                 860                 
Leu Gly Asn Val Asn Phe Thr Val Ser Ala Glu Ala Leu Glu Ser Gln 
865                 870                 875                 880 
Glu Leu Cys Gly Thr Glu Val Pro Ser Val Pro Glu His Gly Arg Lys 
                885                 890                 895     
Asp Thr Val Ile Lys Pro Leu Leu Val Glu Pro Glu Gly Leu Glu Lys 
            900                 905                 910         
Glu Thr Thr Phe Asn Ser Leu Leu Cys Pro Ser Gly Gly Glu Val Ser 
        915                 920                 925             
Glu Glu Leu Ser Leu Lys Leu Pro Pro Asn Val Val Glu Glu Ser Ala 
    930                 935                 940                 
Arg Ala Ser Val Ser Val Leu Gly Asp Ile Leu Gly Ser Ala Met Gln 
945                 950                 955                 960 
Asn Thr Gln Asn Leu Leu Gln Met Pro Tyr Gly Cys Gly Glu Gln Asn 
                965                 970                 975     
Met Val Leu Phe Ala Pro Asn Ile Tyr Val Leu Asp Tyr Leu Asn Glu 
            980                 985                 990         
Thr Gln Gln Leu Thr Pro Glu Ile Lys Ser Lys Ala Ile Gly Tyr Leu 
        995                 1000                1005            
Asn Thr Gly Tyr Gln Arg Gln Leu Asn Tyr Lys His Tyr Asp Gly Ser 
    1010                1015                1020                
Tyr Ser Thr Phe Gly Glu Arg Tyr Gly Arg Asn Gln Gly Asn Thr Trp 
1025                1030                1035                1040
Leu Thr Ala Phe Val Leu Lys Thr Phe Ala Gln Ala Arg Ala Tyr Ile 
                1045                1050                1055    
Phe Ile Asp Glu Ala His Ile Thr Gln Ala Leu Ile Trp Leu Ser Gln 
            1060                1065                1070        
Arg Gln Lys Asp Asn Gly Cys Phe Arg Ser Ser Gly Ser Leu Leu Asn 
        1075                1080                1085            
Asn Ala Ile Lys Gly Gly Val Glu Asp Glu Val Thr Leu Ser Ala Tyr 
    1090                1095                1100                
Ile Thr Ile Ala Leu Leu Glu Ile Pro Leu Thr Val Thr His Pro Val 
1105                1110                1115                1120
Val Arg Asn Ala Leu Phe Cys Leu Glu Ser Ala Trp Lys Thr Ala Gln 
                1125                1130                1135    
Glu Gly Asp His Gly Ser His Val Tyr Thr Lys Ala Leu Leu Ala Tyr 
            1140                1145                1150        
Ala Phe Ala Leu Ala Gly Asn Gln Asp Lys Arg Lys Glu Val Leu Lys 
        1155                1160                1165            
Ser Leu Asn Glu Glu Ala Val Lys Lys Asp Asn Ser Val His Trp Glu 
    1170                1175                1180                
Arg Pro Gln Lys Pro Lys Ala Pro Val Gly His Phe Tyr Glu Pro Gln 
1185                1190                1195                1200
Ala Pro Ser Ala Glu Val Glu Met Thr Ser Tyr Val Leu Leu Ala Tyr 
                1205                1210                1215    
Leu Thr Ala Gln Pro Ala Pro Thr Ser Glu Asp Leu Thr Ser Ala Thr 
            1220                1225                1230        
Asn Ile Val Lys Trp Ile Thr Lys Gln Gln Asn Ala Gln Gly Gly Phe 
        1235                1240                1245            
Ser Ser Thr Gln Asp Thr Val Val Ala Leu His Ala Leu Ser Lys Tyr 
    1250                1255                1260                
Gly Ala Ala Thr Phe Thr Arg Thr Gly Lys Ala Ala Gln Val Thr Ile 
1265                1270                1275                1280
Gln Ser Ser Gly Thr Phe Ser Ser Lys Phe Gln Val Asp Asn Asn Asn 
                1285                1290                1295    
Arg Leu Leu Leu Gln Gln Val Ser Leu Pro Glu Leu Pro Gly Glu Tyr 
            1300                1305                1310        
Ser Met Lys Val Thr Gly Glu Gly Cys Val Tyr Leu Gln Thr Ser Leu 
        1315                1320                1325            
Lys Tyr Asn Ile Leu Pro Glu Lys Glu Glu Phe Pro Phe Ala Leu Gly 
    1330                1335                1340                
Val Gln Thr Leu Pro Gln Thr Cys Asp Glu Pro Lys Ala His Thr Ser 
1345                1350                1355                1360
Phe Gln Ile Ser Leu Ser Val Ser Tyr Thr Gly Ser Arg Ser Ala Ser 
                1365                1370                1375    
Asn Met Ala Ile Val Asp Val Lys Met Val Ser Gly Phe Ile Pro Leu 
            1380                1385                1390        
Lys Pro Thr Val Lys Met Leu Glu Arg Ser Asn His Val Ser Arg Thr 
        1395                1400                1405            
Glu Val Ser Ser Asn His Val Leu Ile Tyr Leu Asp Lys Val Ser Asn 
    1410                1415                1420                
Gln Thr Leu Ser Leu Phe Phe Thr Val Leu Gln Asp Val Pro Val Arg 
1425                1430                1435                1440
Asp Leu Lys Pro Ala Ile Val Lys Val Tyr Asp Tyr Tyr Glu Thr Asp 
                1445                1450                1455    
Glu Phe Ala Ile Ala Glu Tyr Asn Ala Pro Cys Ser Lys Asp Leu Gly 
            1460                1465                1470        
Asn Ala 
        

