               SEQUENCE LISTING

<110> DSM IP Assets B.V.

<120> Lipolytic enzyme variants

<130> 32419-WO-PCT

<150> EP17157059.1
<151> 2017-02-21
<150> EP16205903.4
<151> 2016-12-21

<160> 7

<170> BiSSAP 1.3.6

<210> 1
<211> 1038
<212> DNA
<213> Artificial sequence


<220> 
<223> DNA lipolytic enzyme gene

<400> 1
atgcttctcc tctccctcct ctccattgtc accctcgctg ttgcttctcc tctgtccgtt      60

gaggagtacg ccaaggccct cgaggagcgt gccgtcaccg tctcctcctc cgagctcaac     120

aacttcaagt tctacatcca gcacggtgct gctgcctact gcaactccga gactgctgct     180

ggtgccaacg tcacctgcac tggcaacgcc tgccccgaga ttgaggccaa cggtgtcacc     240

gttgttgcct ccttcactgg taccaagact ggtatcggtg gctacgtctc caccgacaac     300

accaacaagg agatcgtcct ttctttccgt ggcagcatca acatccgcaa ctggctgacc     360

aacctggact tcggccagga tgactgctct ctgacctccg gctgcggtgt ccactccggt     420

ttccagcgtg cctgggagga gattgccgac aacctgaccg ctgctgttgc caaggccaag     480

actgccaacc ccgactacaa ggttgttgcc actggccact ccctgggtgg tgctgttgcc     540

accctggctg gtgccaacct ccgtgctgct ggtacccccc tcgacatcta cacctacggc     600

tctccccgtg tcggcaacgc cgagcttgct gagttcatct ccaaccagac tggtggtgag     660

ttccgtgtca cccacggtga tgaccccgtc ccccgtcttc ctcctctgat cttcggctac     720

cgccacacct cccccgagta ctggctcgat ggcagcggtg gtgacaagat caactacacc     780

atcaacgaca tcaaggtctg cgagggtgct gccaacctgc agtgcaacgg tggtaccctg     840

ggtctcgaca ttgctgctca cctgcactac ttccaggcca ctgatgcctg caacgccggt     900

ggtttcagct ggcgccgcta ccgctctgct gagagcgttg acaagcgtgc caccatgact     960

gatgctgagc tcgagaagaa gctcaacagc tacgtgcaga tggacaagga gtacgtcaag    1020

aacaaccagg ctcgctcc                                                  1038


<210> 2
<211> 346
<212> PRT
<213> Artificial sequence


<220> 
<223> Lipolytic enzyme protein

<400> 2
Met Leu Leu Leu Ser Leu Leu Ser Ile Val Thr Leu Ala Val Ala Ser 
1               5                   10                  15      
Pro Leu Ser Val Glu Glu Tyr Ala Lys Ala Leu Glu Glu Arg Ala Val 
            20                  25                  30          
Thr Val Ser Ser Ser Glu Leu Asn Asn Phe Lys Phe Tyr Ile Gln His 
        35                  40                  45              
Gly Ala Ala Ala Tyr Cys Asn Ser Glu Thr Ala Ala Gly Ala Asn Val 
    50                  55                  60                  
Thr Cys Thr Gly Asn Ala Cys Pro Glu Ile Glu Ala Asn Gly Val Thr 
65                  70                  75                  80  
Val Val Ala Ser Phe Thr Gly Thr Lys Thr Gly Ile Gly Gly Tyr Val 
                85                  90                  95      
Ser Thr Asp Asn Thr Asn Lys Glu Ile Val Leu Ser Phe Arg Gly Ser 
            100                 105                 110         
Ile Asn Ile Arg Asn Trp Leu Thr Asn Leu Asp Phe Gly Gln Asp Asp 
        115                 120                 125             
Cys Ser Leu Thr Ser Gly Cys Gly Val His Ser Gly Phe Gln Arg Ala 
    130                 135                 140                 
Trp Glu Glu Ile Ala Asp Asn Leu Thr Ala Ala Val Ala Lys Ala Lys 
145                 150                 155                 160 
Thr Ala Asn Pro Asp Tyr Lys Val Val Ala Thr Gly His Ser Leu Gly 
                165                 170                 175     
Gly Ala Val Ala Thr Leu Ala Gly Ala Asn Leu Arg Ala Ala Gly Thr 
            180                 185                 190         
Pro Leu Asp Ile Tyr Thr Tyr Gly Ser Pro Arg Val Gly Asn Ala Glu 
        195                 200                 205             
Leu Ala Glu Phe Ile Ser Asn Gln Thr Gly Gly Glu Phe Arg Val Thr 
    210                 215                 220                 
His Gly Asp Asp Pro Val Pro Arg Leu Pro Pro Leu Ile Phe Gly Tyr 
225                 230                 235                 240 
Arg His Thr Ser Pro Glu Tyr Trp Leu Asp Gly Ser Gly Gly Asp Lys 
                245                 250                 255     
Ile Asn Tyr Thr Ile Asn Asp Ile Lys Val Cys Glu Gly Ala Ala Asn 
            260                 265                 270         
Leu Gln Cys Asn Gly Gly Thr Leu Gly Leu Asp Ile Ala Ala His Leu 
        275                 280                 285             
His Tyr Phe Gln Ala Thr Asp Ala Cys Asn Ala Gly Gly Phe Ser Trp 
    290                 295                 300                 
Arg Arg Tyr Arg Ser Ala Glu Ser Val Asp Lys Arg Ala Thr Met Thr 
305                 310                 315                 320 
Asp Ala Glu Leu Glu Lys Lys Leu Asn Ser Tyr Val Gln Met Asp Lys 
                325                 330                 335     
Glu Tyr Val Lys Asn Asn Gln Ala Arg Ser 
            340                 345     

<210> 3
<211> 13
<212> DNA
<213> Artificial Sequence


<220> 
<223> Sets out a modified translational initiation sequence of the
      glucoamylase glaA promoter

<400> 3
caccgtcaaa atg                                                       13


<210> 4
<211> 686
<212> PRT
<213> Alicyclobacillus pohliae


<400> 4
Ser Ser Ser Ala Ser Val Lys Gly Asp Val Ile Tyr Gln Ile Ile Ile 
1               5                   10                  15      
Asp Arg Phe Tyr Asp Gly Asp Thr Thr Asn Asn Asn Pro Ala Lys Ser 
            20                  25                  30          
Tyr Gly Leu Tyr Asp Pro Thr Lys Ser Lys Trp Lys Met Tyr Trp Gly 
        35                  40                  45              
Gly Asp Leu Glu Gly Val Arg Gln Lys Leu Pro Tyr Leu Lys Gln Leu 
    50                  55                  60                  
Gly Val Thr Thr Ile Trp Leu Ser Pro Val Leu Asp Asn Leu Asp Thr 
65                  70                  75                  80  
Leu Ala Gly Thr Asp Asn Thr Gly Tyr His Gly Tyr Trp Thr Arg Asp 
                85                  90                  95      
Phe Lys Gln Ile Glu Glu His Phe Gly Asn Trp Thr Thr Phe Asp Thr 
            100                 105                 110         
Leu Val Asn Asp Ala His Gln Asn Gly Ile Lys Val Ile Val Asp Phe 
        115                 120                 125             
Val Pro Asn His Ser Thr Pro Phe Lys Ala Asn Asp Ser Thr Phe Ala 
    130                 135                 140                 
Glu Gly Gly Ala Leu Tyr Asp Asn Gly Thr Tyr Met Gly Asn Tyr Phe 
145                 150                 155                 160 
Asp Asp Ala Thr Lys Gly Tyr Phe His His Asn Gly Asp Ile Ser Asn 
                165                 170                 175     
Trp Asp Asp Arg Tyr Glu Ala Gln Trp Lys Asn Phe Thr Asp Pro Ala 
            180                 185                 190         
Gly Phe Ser Leu Ala Asp Leu Ser Gln Glu Asn Gly Thr Ile Ala Gln 
        195                 200                 205             
Tyr Leu Thr Asp Ala Ala Val Gln Leu Val Ala His Gly Ala Asp Gly 
    210                 215                 220                 
Leu Arg Ile Asp Ala Val Lys His Phe Asn Ser Gly Phe Ser Lys Ser 
225                 230                 235                 240 
Leu Ala Asp Lys Leu Tyr Gln Lys Lys Asp Ile Phe Leu Val Gly Glu 
                245                 250                 255     
Trp Tyr Gly Asp Asp Pro Gly Ala Ala Asn His Leu Glu Lys Val Arg 
            260                 265                 270         
Tyr Ala Asn Asn Ser Gly Val Asn Val Leu Asp Phe Asp Leu Asn Thr 
        275                 280                 285             
Val Ile Arg Asn Val Phe Gly Thr Phe Thr Gln Thr Met Tyr Asp Leu 
    290                 295                 300                 
Asn Asn Met Val Asn Gln Thr Gly Asn Glu Tyr Lys Tyr Lys Glu Asn 
305                 310                 315                 320 
Leu Ile Thr Phe Ile Asp Asn His Asp Met Ser Arg Phe Leu Thr Val 
                325                 330                 335     
Asn Ser Asn Lys Ala Asn Leu His Gln Ala Leu Ala Phe Ile Leu Thr 
            340                 345                 350         
Ser Arg Gly Thr Pro Ser Ile Tyr Tyr Gly Thr Glu Gln Tyr Met Ala 
        355                 360                 365             
Gly Gly Asn Asp Pro Tyr Asn Arg Gly Met Met Pro Ala Phe Asp Thr 
    370                 375                 380                 
Thr Thr Thr Ala Phe Lys Glu Val Ser Thr Leu Ala Gly Leu Arg Arg 
385                 390                 395                 400 
Asn Asn Ala Ala Ile Gln Tyr Gly Thr Thr Thr Gln Arg Trp Ile Asn 
                405                 410                 415     
Asn Asp Val Tyr Ile Tyr Glu Arg Lys Phe Phe Asn Asp Val Val Leu 
            420                 425                 430         
Val Ala Ile Asn Arg Asn Thr Gln Ser Ser Tyr Ser Ile Ser Gly Leu 
        435                 440                 445             
Gln Thr Ala Leu Pro Asn Gly Asn Tyr Ala Asp Tyr Leu Ser Gly Leu 
    450                 455                 460                 
Leu Gly Gly Asn Gly Ile Ser Val Ser Asn Gly Ser Val Ala Ser Phe 
465                 470                 475                 480 
Thr Leu Ala Pro Gly Ala Val Ser Val Trp Gln Tyr Ser Thr Ser Ala 
                485                 490                 495     
Ser Ala Pro Gln Ile Gly Ser Val Ala Pro Asn Met Gly Ile Pro Gly 
            500                 505                 510         
Asn Val Val Thr Ile Asp Gly Lys Gly Phe Gly Thr Thr Gln Gly Thr 
        515                 520                 525             
Val Thr Phe Gly Gly Val Thr Ala Thr Val Lys Ser Trp Thr Ser Asn 
    530                 535                 540                 
Arg Ile Glu Val Tyr Val Pro Asn Met Ala Ala Gly Leu Thr Asp Val 
545                 550                 555                 560 
Lys Val Thr Ala Gly Gly Val Ser Ser Asn Leu Tyr Ser Tyr Asn Ile 
                565                 570                 575     
Leu Ser Gly Thr Gln Thr Ser Val Val Phe Thr Val Lys Ser Ala Pro 
            580                 585                 590         
Pro Thr Asn Leu Gly Asp Lys Ile Tyr Leu Thr Gly Asn Ile Pro Glu 
        595                 600                 605             
Leu Gly Asn Trp Ser Thr Asp Thr Ser Gly Ala Val Asn Asn Ala Gln 
    610                 615                 620                 
Gly Pro Leu Leu Ala Pro Asn Tyr Pro Asp Trp Phe Tyr Val Phe Ser 
625                 630                 635                 640 
Val Pro Ala Gly Lys Thr Ile Gln Phe Lys Phe Phe Ile Lys Arg Ala 
                645                 650                 655     
Asp Gly Thr Ile Gln Trp Glu Asn Gly Ser Asn His Val Ala Thr Thr 
            660                 665                 670         
Pro Thr Gly Ala Thr Gly Asn Ile Thr Val Thr Trp Gln Asn 
        675                 680                 685     

<210> 5
<211> 686
<212> PRT
<213> Bacillus stearothermophilus


<400> 5
Ser Ser Ser Ala Ser Val Lys Gly Asp Val Ile Tyr Gln Ile Ile Ile 
1               5                   10                  15      
Asp Arg Phe Tyr Asp Gly Asp Thr Thr Asn Asn Asn Pro Ala Lys Ser 
            20                  25                  30          
Tyr Gly Leu Tyr Asp Pro Thr Lys Ser Lys Trp Lys Met Tyr Trp Gly 
        35                  40                  45              
Gly Asp Leu Glu Gly Val Arg Gln Lys Leu Pro Tyr Leu Lys Gln Leu 
    50                  55                  60                  
Gly Val Thr Thr Ile Trp Leu Ser Pro Val Leu Asp Asn Leu Asp Thr 
65                  70                  75                  80  
Leu Ala Gly Thr Asp Asn Thr Gly Tyr His Gly Tyr Trp Thr Arg Asp 
                85                  90                  95      
Phe Lys Gln Ile Glu Glu His Phe Gly Asn Trp Thr Thr Phe Asp Thr 
            100                 105                 110         
Leu Val Asn Asp Ala His Gln Asn Gly Ile Lys Val Ile Val Asp Phe 
        115                 120                 125             
Val Pro Asn His Ser Thr Pro Phe Lys Ala Asn Asp Ser Thr Phe Ala 
    130                 135                 140                 
Glu Gly Gly Ala Leu Tyr Asn Asn Gly Thr Tyr Met Gly Asn Tyr Phe 
145                 150                 155                 160 
Asp Asp Ala Thr Lys Gly Tyr Phe His His Asn Gly Asp Ile Ser Asn 
                165                 170                 175     
Trp Asp Asp Arg Tyr Glu Ala Gln Trp Lys Asn Phe Thr Asp Pro Ala 
            180                 185                 190         
Gly Phe Ser Leu Ala Asp Leu Ser Gln Glu Asn Gly Thr Ile Ala Gln 
        195                 200                 205             
Tyr Leu Thr Asp Ala Ala Val Gln Leu Val Ala His Gly Ala Asp Gly 
    210                 215                 220                 
Leu Arg Ile Asp Ala Val Lys His Phe Asn Ser Gly Phe Ser Lys Ser 
225                 230                 235                 240 
Leu Ala Asp Lys Leu Tyr Gln Lys Lys Asp Ile Phe Leu Val Gly Glu 
                245                 250                 255     
Trp Tyr Gly Asp Asp Pro Gly Thr Ala Asn His Leu Glu Lys Val Arg 
            260                 265                 270         
Tyr Ala Asn Asn Ser Gly Val Asn Val Leu Asp Phe Asp Leu Asn Thr 
        275                 280                 285             
Val Ile Arg Asn Val Phe Gly Thr Phe Thr Gln Thr Met Tyr Asp Leu 
    290                 295                 300                 
Asn Asn Met Val Asn Gln Thr Gly Asn Glu Tyr Lys Tyr Lys Glu Asn 
305                 310                 315                 320 
Leu Ile Thr Phe Ile Asp Asn His Asp Met Ser Arg Phe Leu Ser Val 
                325                 330                 335     
Asn Ser Asn Lys Ala Asn Leu His Gln Ala Leu Ala Phe Ile Leu Thr 
            340                 345                 350         
Ser Arg Gly Thr Pro Ser Ile Tyr Tyr Gly Thr Glu Gln Tyr Met Ala 
        355                 360                 365             
Gly Gly Asn Asp Pro Tyr Asn Arg Gly Met Met Pro Ala Phe Asp Thr 
    370                 375                 380                 
Thr Thr Thr Ala Phe Lys Glu Val Ser Thr Leu Ala Gly Leu Arg Arg 
385                 390                 395                 400 
Asn Asn Ala Ala Ile Gln Tyr Gly Thr Thr Thr Gln Arg Trp Ile Asn 
                405                 410                 415     
Asn Asp Val Tyr Ile Tyr Glu Arg Lys Phe Phe Asn Asp Val Val Leu 
            420                 425                 430         
Val Ala Ile Asn Arg Asn Thr Gln Ser Ser Tyr Ser Ile Ser Gly Leu 
        435                 440                 445             
Gln Thr Ala Leu Pro Asn Gly Ser Tyr Ala Asp Tyr Leu Ser Gly Leu 
    450                 455                 460                 
Leu Gly Gly Asn Gly Ile Ser Val Ser Asn Gly Ser Val Ala Ser Phe 
465                 470                 475                 480 
Thr Leu Ala Pro Gly Ala Val Ser Val Trp Gln Tyr Ser Thr Ser Ala 
                485                 490                 495     
Ser Ala Pro Gln Ile Gly Ser Val Ala Pro Asn Met Gly Ile Pro Gly 
            500                 505                 510         
Asn Val Val Thr Ile Asp Gly Lys Gly Phe Gly Thr Thr Gln Gly Thr 
        515                 520                 525             
Val Thr Phe Gly Gly Val Thr Ala Thr Val Lys Ser Trp Thr Ser Asn 
    530                 535                 540                 
Arg Ile Glu Val Tyr Val Pro Asn Met Ala Ala Gly Leu Thr Asp Val 
545                 550                 555                 560 
Lys Val Thr Ala Gly Gly Val Ser Ser Asn Leu Tyr Ser Tyr Asn Ile 
                565                 570                 575     
Leu Ser Gly Thr Gln Thr Ser Val Val Phe Thr Val Lys Ser Ala Pro 
            580                 585                 590         
Pro Thr Asn Leu Gly Asp Lys Ile Tyr Leu Thr Gly Asn Ile Pro Glu 
        595                 600                 605             
Leu Gly Asn Trp Ser Thr Asp Thr Ser Gly Ala Val Asn Asn Ala Gln 
    610                 615                 620                 
Gly Pro Leu Leu Ala Pro Asn Tyr Pro Asp Trp Phe Tyr Val Phe Ser 
625                 630                 635                 640 
Val Pro Ala Gly Lys Thr Ile Gln Phe Lys Phe Phe Ile Lys Arg Ala 
                645                 650                 655     
Asp Gly Thr Ile Gln Trp Glu Asn Gly Ser Asn His Val Ala Thr Thr 
            660                 665                 670         
Pro Thr Gly Ala Thr Gly Asn Ile Thr Val Thr Trp Gln Asn 
        675                 680                 685     

<210> 6
<211> 429
<212> PRT
<213> Pseudomonas saccharophilia


<400> 6
Asp Gln Ala Gly Lys Ser Pro Ala Gly Val Arg Tyr His Gly Gly Asp 
1               5                   10                  15      
Glu Ile Ile Leu Gln Gly Phe His Trp Asn Val Val Arg Glu Ala Pro 
            20                  25                  30          
Asn Asp Trp Tyr Asn Ile Leu Arg Gln Gln Ala Ser Thr Ile Ala Ala 
        35                  40                  45              
Asp Gly Phe Ser Ala Ile Trp Met Pro Val Pro Trp Arg Asp Phe Ser 
    50                  55                  60                  
Ser Trp Thr Asp Gly Gly Lys Ser Gly Gly Gly Glu Gly Tyr Phe Trp 
65                  70                  75                  80  
His Asp Phe Asn Lys Asn Gly Arg Tyr Gly Ser Asp Ala Gln Leu Arg 
                85                  90                  95      
Gln Ala Ala Gly Ala Leu Gly Gly Ala Gly Val Lys Val Leu Tyr Asp 
            100                 105                 110         
Val Val Pro Asn His Met Asn Arg Gly Tyr Pro Asp Lys Glu Ile Asn 
        115                 120                 125             
Leu Pro Ala Gly Gln Gly Phe Trp Arg Asn Asp Cys Ala Asp Pro Gly 
    130                 135                 140                 
Asn Tyr Pro Asn Asp Cys Asp Asp Gly Asp Arg Phe Ile Gly Gly Glu 
145                 150                 155                 160 
Ser Asp Leu Asn Thr Gly His Pro Gln Ile Tyr Gly Met Phe Arg Asp 
                165                 170                 175     
Glu Leu Ala Asn Leu Arg Ser Gly Tyr Gly Ala Gly Gly Phe Arg Phe 
            180                 185                 190         
Asp Phe Val Arg Gly Tyr Ala Pro Glu Arg Val Asp Ser Trp Met Ser 
        195                 200                 205             
Asp Ser Ala Asp Ser Ser Phe Cys Val Gly Glu Leu Trp Lys Gly Pro 
    210                 215                 220                 
Ser Glu Tyr Pro Ser Trp Asp Trp Arg Asn Thr Ala Ser Trp Gln Gln 
225                 230                 235                 240 
Ile Ile Lys Asp Trp Ser Asp Arg Ala Lys Cys Pro Val Phe Asp Phe 
                245                 250                 255     
Ala Leu Lys Glu Arg Met Gln Asn Gly Ser Val Ala Asp Trp Lys His 
            260                 265                 270         
Gly Leu Asn Gly Asn Pro Asp Pro Arg Trp Arg Glu Val Ala Val Thr 
        275                 280                 285             
Phe Val Asp Asn His Asp Thr Gly Tyr Ser Pro Gly Gln Asn Gly Gly 
    290                 295                 300                 
Gln His His Trp Ala Leu Gln Asp Gly Leu Ile Arg Gln Ala Tyr Ala 
305                 310                 315                 320 
Tyr Ile Leu Thr Ser Pro Gly Thr Pro Val Val Tyr Trp Ser His Met 
                325                 330                 335     
Tyr Asp Trp Gly Tyr Gly Asp Phe Ile Arg Gln Leu Ile Gln Val Arg 
            340                 345                 350         
Arg Thr Ala Gly Val Arg Ala Asp Ser Ala Ile Ser Phe His Ser Gly 
        355                 360                 365             
Tyr Ser Gly Leu Val Ala Thr Val Ser Gly Ser Gln Gln Thr Leu Val 
    370                 375                 380                 
Val Ala Leu Asn Ser Asp Leu Ala Asn Pro Gly Gln Val Ala Ser Gly 
385                 390                 395                 400 
Ser Phe Ser Glu Ala Val Asn Ala Ser Asn Gly Gln Val Arg Val Trp 
                405                 410                 415     
Arg Ser Gly Ser Gly Asp Gly Gly Gly Asn Asp Gly Gly 
            420                 425                 

<210> 7
<211> 429
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic polypeptide

<400> 7
Asp Gln Ala Gly Lys Ser Pro Ala Gly Val Arg Tyr His Gly Gly Asp 
1               5                   10                  15      
Glu Ile Ile Leu Gln Gly Phe His Trp Asn Val Val Arg Glu Ala Pro 
            20                  25                  30          
Tyr Asn Trp Tyr Asn Ile Leu Arg Gln Gln Ala Ser Thr Ile Ala Ala 
        35                  40                  45              
Asp Gly Phe Ser Ala Ile Trp Met Pro Val Pro Trp Arg Asp Phe Ser 
    50                  55                  60                  
Ser Trp Thr Asp Gly Asp Lys Ser Gly Gly Gly Glu Gly Tyr Phe Trp 
65                  70                  75                  80  
His Asp Phe Asn Lys Asn Gly Arg Tyr Gly Ser Asp Ala Gln Leu Arg 
                85                  90                  95      
Gln Ala Ala Gly Ala Leu Gly Gly Ala Gly Val Lys Val Leu Tyr Asp 
            100                 105                 110         
Val Val Pro Asn His Met Asn Arg Phe Tyr Pro Asp Lys Glu Ile Asn 
        115                 120                 125             
Leu Pro Ala Gly Gln Arg Phe Trp Arg Asn Asp Cys Pro Asp Pro Gly 
    130                 135                 140                 
Asn Gly Pro Asn Asp Cys Asp Asp Gly Asp Arg Phe Leu Gly Gly Glu 
145                 150                 155                 160 
Ala Asp Leu Asn Thr Gly His Pro Gln Ile Tyr Gly Met Phe Arg Asp 
                165                 170                 175     
Glu Phe Thr Asn Leu Arg Ser Gly Tyr Gly Ala Gly Gly Phe Arg Phe 
            180                 185                 190         
Asp Phe Val Arg Gly Tyr Ala Pro Glu Arg Val Asp Ser Trp Met Ser 
        195                 200                 205             
Asp Ser Ala Asp Ser Ser Phe Cys Val Gly Glu Leu Trp Lys Glu Pro 
    210                 215                 220                 
Ser Glu Tyr Pro Pro Trp Asp Trp Arg Asn Thr Ala Ser Trp Gln Gln 
225                 230                 235                 240 
Ile Ile Lys Asp Trp Ser Asp Arg Ala Lys Cys Pro Val Phe Asp Phe 
                245                 250                 255     
Ala Leu Lys Glu Arg Met Gln Asn Gly Ser Val Ala Asp Trp Lys His 
            260                 265                 270         
Gly Leu Asn Gly Asn Pro Asp Pro Arg Trp Arg Glu Val Ala Val Thr 
        275                 280                 285             
Phe Val Asp Asn His Asp Thr Gly Tyr Ser Pro Gly Gln Asn Gly Gly 
    290                 295                 300                 
Gln His Lys Trp Pro Leu Gln Asp Gly Leu Ile Arg Gln Ala Tyr Ala 
305                 310                 315                 320 
Tyr Ile Leu Thr Ser Pro Gly Thr Pro Val Val Tyr Trp Pro His Met 
                325                 330                 335     
Tyr Asp Trp Gly Tyr Gly Asp Phe Ile Arg Gln Leu Ile Gln Val Arg 
            340                 345                 350         
Arg Thr Ala Gly Val Arg Ala Asp Ser Ala Ile Ser Phe His Ser Gly 
        355                 360                 365             
Tyr Ser Gly Leu Val Ala Thr Val Ser Gly Ser Gln Gln Thr Leu Val 
    370                 375                 380                 
Val Ala Leu Asn Ser Asp Leu Ala Asn Pro Gly Gln Val Ala Ser Gly 
385                 390                 395                 400 
Ser Phe Ser Glu Ala Val Asn Ala Ser Asn Gly Gln Val Arg Val Trp 
                405                 410                 415     
Arg Ser Gly Ser Gly Asp Gly Gly Gly Asn Asp Gly Gly 
            420                 425                 

