                         SEQUENCE LISTING

<110>  Merck Sharp & Dohme Corp.
       Meyer, Brian K.
       Bhambhani, Akhilesh
       Blue, Jeffrey T.
 
<120>  A CELL-BASED REPORTER ASSAY FOR LIVE VIRUS VACCINES

<130>  24406

<150>  62/434,675
<151>  2016-12-15

<160>  5

<170>  PatentIn version 3.5

<210>  1
<211>  1378
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NanoLuc Reporter Gene

<400>  1
aagcttattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata       60

cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc      120

caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg      180

gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca      240

tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc      300

ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt      360

attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata      420

gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt      480

ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca      540

aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag      600

agaacccact gcttactggc ttatcgaaat taatacgact cactataggg gcaatccggt      660

actgttggta aagccaccat ggtcttcaca ctcgaagatt tcgttgggga ctggcgacag      720

acagccggct acaacctgga ccaagtcctt gaacagggag gtgtgtccag tttgtttcag      780

aatctcgggg tgtccgtaac tccgatccaa aggattgtcc tgagcggtga aaatgggctg      840

aagatcgaca tccatgtcat catcccgtat gaaggtctga gcggcgacca aatgggccag      900

atcgaaaaaa tttttaaggt ggtgtaccct gtggatgatc atcactttaa ggtgatcctg      960

cactatggca cactggtaat cgacggggtt acgccgaaca tgatcgacta tttcggacgg     1020

ccgtatgaag gcatcgccgt gttcgacggc aaaaagatca ctgtaacagg gaccctgtgg     1080

aacggcaaca aaattatcga cgagcgcctg atcaaccccg acggctccct gctgttccga     1140

gtaaccatca acggagtgac cggctggcgg ctgtgcgaac gcattctggc gtaaggccgc     1200

gactctagag tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt     1260

ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg     1320

ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattg       1378


<210>  2
<211>  578
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CMV immediate/early enhancer/promoter

<400>  2
attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat       60

ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc      120

ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca      180

ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta      240

tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta      300

tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat      360

cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga      420

ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca      480

aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg      540

taggcgtgta cggtgggagg tctatataag cagagctc                              578


<210>  3
<211>  516
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NanoLuc Enzyme (DNA)

<400>  3
atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg       60

gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta      120

actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc      180

atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag      240

gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta      300

atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc      360

gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc      420

gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg      480

accggctggc ggctgtgcga acgcattctg gcgtaa                                516


<210>  4
<211>  171
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NanoLuc Enzyme (Protein)

<400>  4

Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 


Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala 
                165                 170     


<210>  5
<211>  550
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Luc2 Enzyme (Protein)

<400>  5

Met Glu Asp Ala Lys Asn Ile Lys Lys Gly Pro Ala Pro Phe Tyr Pro 
1               5                   10                  15      


Leu Glu Asp Gly Thr Ala Gly Glu Gln Leu His Lys Ala Met Lys Arg 
            20                  25                  30          


Tyr Ala Leu Val Pro Gly Thr Ile Ala Phe Thr Asp Ala His Ile Glu 
        35                  40                  45              


Val Asp Ile Thr Tyr Ala Glu Tyr Phe Glu Met Ser Val Arg Leu Ala 
    50                  55                  60                  


Glu Ala Met Lys Arg Tyr Gly Leu Asn Thr Asn His Arg Ile Val Val 
65                  70                  75                  80  


Cys Ser Glu Asn Ser Leu Gln Phe Phe Met Pro Val Leu Gly Ala Leu 
                85                  90                  95      


Phe Ile Gly Val Ala Val Ala Pro Ala Asn Asp Ile Tyr Asn Glu Arg 
            100                 105                 110         


Glu Leu Leu Asn Ser Met Gly Ile Ser Gln Pro Thr Val Val Phe Val 
        115                 120                 125             


Ser Lys Lys Gly Leu Gln Lys Ile Leu Asn Val Gln Lys Lys Leu Pro 
    130                 135                 140                 


Ile Ile Gln Lys Ile Ile Ile Met Asp Ser Lys Thr Asp Tyr Gln Gly 
145                 150                 155                 160 


Phe Gln Ser Met Tyr Thr Phe Val Thr Ser His Leu Pro Pro Gly Phe 
                165                 170                 175     


Asn Glu Tyr Asp Phe Val Pro Glu Ser Phe Asp Arg Asp Lys Thr Ile 
            180                 185                 190         


Ala Leu Ile Met Asn Ser Ser Gly Ser Thr Gly Leu Pro Lys Gly Val 
        195                 200                 205             


Ala Leu Pro His Arg Thr Ala Cys Val Arg Phe Ser His Ala Arg Asp 
    210                 215                 220                 


Pro Ile Phe Gly Asn Gln Ile Ile Pro Asp Thr Ala Ile Leu Ser Val 
225                 230                 235                 240 


Val Pro Phe His His Gly Phe Gly Met Phe Thr Thr Leu Gly Tyr Leu 
                245                 250                 255     


Ile Cys Gly Phe Arg Val Val Leu Met Tyr Arg Phe Glu Glu Glu Leu 
            260                 265                 270         


Phe Leu Arg Ser Leu Gln Asp Tyr Lys Ile Gln Ser Ala Leu Leu Val 
        275                 280                 285             


Pro Thr Leu Phe Ser Phe Phe Ala Lys Ser Thr Leu Ile Asp Lys Tyr 
    290                 295                 300                 


Asp Leu Ser Asn Leu His Glu Ile Ala Ser Gly Gly Ala Pro Leu Ser 
305                 310                 315                 320 


Lys Glu Val Gly Glu Ala Val Ala Lys Arg Phe His Leu Pro Gly Ile 
                325                 330                 335     


Arg Gln Gly Tyr Gly Leu Thr Glu Thr Thr Ser Ala Ile Leu Ile Thr 
            340                 345                 350         


Pro Glu Gly Asp Asp Lys Pro Gly Ala Val Gly Lys Val Val Pro Phe 
        355                 360                 365             


Phe Glu Ala Lys Val Val Asp Leu Asp Thr Gly Lys Thr Leu Gly Val 
    370                 375                 380                 


Asn Gln Arg Gly Glu Leu Cys Val Arg Gly Pro Met Ile Met Ser Gly 
385                 390                 395                 400 


Tyr Val Asn Asn Pro Glu Ala Thr Asn Ala Leu Ile Asp Lys Asp Gly 
                405                 410                 415     


Trp Leu His Ser Gly Asp Ile Ala Tyr Trp Asp Glu Asp Glu His Phe 
            420                 425                 430         


Phe Ile Val Asp Arg Leu Lys Ser Leu Ile Lys Tyr Lys Gly Tyr Gln 
        435                 440                 445             


Val Ala Pro Ala Glu Leu Glu Ser Ile Leu Leu Gln His Pro Asn Ile 
    450                 455                 460                 


Phe Asp Ala Gly Val Ala Gly Leu Pro Asp Asp Asp Ala Gly Glu Leu 
465                 470                 475                 480 


Pro Ala Ala Val Val Val Leu Glu His Gly Lys Thr Met Thr Glu Lys 
                485                 490                 495     


Glu Ile Val Asp Tyr Val Ala Ser Gln Val Thr Thr Ala Lys Lys Leu 
            500                 505                 510         


Arg Gly Gly Val Val Phe Val Asp Glu Val Pro Lys Gly Leu Thr Gly 
        515                 520                 525             


Lys Leu Asp Ala Arg Lys Ile Arg Glu Ile Leu Ile Lys Ala Lys Lys 
    530                 535                 540                 


Gly Gly Lys Ile Ala Val 
545                 550 


