                         SEQUENCE LISTING

<110>  Dow AgroSciences
       Corbin, David
       Chen, Wei
       Lee, Ryan
       Novak, Stephen
       Kumar, Sandeep
       Worden, Andrew
       Asberry, Andrew
 
<120>  Reconstruction of Intact DNA Recognition Sites

<130>  78591-US-PSP

<160>  37    

<170>  PatentIn version 3.5

<210>  1
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  Unique binding site for site specific nuclease

<400>  1
aagactcccg cccatctctc tatgcccggg acaagtg                                37


<210>  2
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Primer sequence

<400>  2
tgacacaaag ctgaaagact at                                                22


<210>  3
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Primer sequence

<400>  3
ttgaggatgt agtaaggagt gg                                                22


<210>  4
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  Primer/probe sequence

<400>  4
acgctggtgc cgtgtat                                                      17


<210>  5
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer sequence

<400>  5
tgttcggttc cctctaccaa                                                   20


<210>  6
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Primer sequence

<400>  6
caacatccat caccttgact ga                                                22


<210>  7
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  primer/probe sequence

<400>  7
cacagaaccg tcgcttcagc aaca                                              24


<210>  8
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Primer sequence

<400>  8
tggttatgac aggctccgtt ta                                                22


<210>  9
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  Primer sequence

<400>  9
aacaaacctc ctggctactt caa                                               23


<210>  10
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  Primer/probe sequence

<400>  10
cttgctggtg ttatgtg                                                      17


<210>  11
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  Primer sequence

<400>  11
acaagagtgg attgatgatc tagagaggt                                         29


<210>  12
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  Primer sequence

<400>  12
ctttgatgcc tatgtgacac gtaaacagt                                         29


<210>  13
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  Primer/probe sequence

<400>  13
ggtgttgtgg ctggtattgc ttacgctgg                                         29


<210>  14
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  Primer sequence

<400>  14
tggcggacga cgacttgt                                                     18


<210>  15
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Primer sequence

<400>  15
aaagtttgga ggctgccgt                                                    19


<210>  16
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Primer/probe sequence

<400>  16
cgagcagacc gccgtgtact t                                                 21


<210>  17
<211>  74
<212>  DNA
<213>  Artificial

<220>
<223>  Sequencing results of the repaired binding site that was cut by a
       site specific nuclease and recombined to produce a binding site 
       that can be recleaved by the same site specific nuclease


<220>
<221>  eZFN2 Binding Site
<222>  (21)..(57)

<400>  17
tggatcgttc tttcatgtag aagactcccg cccatctctc tatgcccggg acaagtggta       60

catgataacc tcag                                                         74


<210>  18
<211>  3453
<212>  DNA
<213>  artificial sequence

<220>
<223>  The AAD-1 Gene expression Cassette that was flanked by the eZFN2 
       Binding Sites


<220>
<221>  eZFN2 binding site
<222>  (1)..(37)

<220>
<221>  eZFN2 binding site
<222>  (3417)..(3453)

<400>  18
aagactcccg cccatctctc tatgcccggg acaagtgggc gcgccaagct tgcatgcctg       60

cagatccccg gggatctccg cggagtgtgc agcgtgaccc ggtcgtgccc ctctctagag      120

ataatgagca ttgcatgtct aagttataaa aaattaccac atattttttt tgtcacactt      180

gtttgaagtg cagtttatct atctttatac atatatttaa actttactct acgaataata      240

taatctatag tactacaata atatcagtgt tttagagaat catataaatg aacagttaga      300

catggtctaa aggacaattg agtattttga caacaggact ctacagtttt atctttttag      360

tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc tatataatac ttcatccatt      420

ttattagtac atccatttag ggtttagggt taatggtttt tatagactaa tttttttagt      480

acatctattt tattctattt tagcctctaa attaagaaaa ctaaaactct attttagttt      540

ttttatttaa tagtttagat ataaaataga ataaaataaa gtgactaaaa attaaacaaa      600

taccctttaa gaaattaaaa aaactaagga aacatttttc ttgtttcgag tagataatgc      660

cagcctgtta aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg      720

cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg acccctctcg      780

agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt gcgtggcgga      840

gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc accggcagct      900

acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc gtaataaata      960

gacaccccct ccacaccctc tttccccaac ctcgtgttgt tcggagcgca cacacacaca     1020

accagatctc ccccaaatcc acccgtcggc acctccgctt caaggtacgc cgctcgtcct     1080

cccccccccc ccccctctct accttctcta gatcggcgtt ccggtccatg catggttagg     1140

gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg     1200

ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat tgctaacttg     1260

ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga cgggatcgat     1320

ttcatgattt tttttgtttc gttgcatagg gtttggtttg cccttttcct ttatttcaat     1380

atatgccgtg cacttgtttg tcgggtcatc ttttcatgct tttttttgtc ttggttgtga     1440

tgatgtggtc tggttgggcg gtcgttctag atcggagtag aattctgttt caaactacct     1500

ggtggattta ttaattttgg atctgtatgt gtgtgccata catattcata gttacgaatt     1560

gaagatgatg gatggaaata tcgatctagg ataggtatac atgttgatgc gggttttact     1620

gatgcatata cagagatgct ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg     1680

gtcgttcatt cgttctagat cggagtagaa tactgtttca aactacctgg tgtatttatt     1740

aattttggaa ctgtatgtgt gtgtcataca tcttcatagt tacgagttta agatggatgg     1800

aaatatcgat ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga     1860

tggcatatgc agcatctatt catatgctct aaccttgagt acctatctat tataataaac     1920

aagtatgttt tataattatt tcgatcttga tatacttgga tgatggcata tgcagcagct     1980

atatgtggat ttttttagcc ctgccttcat acgctattta tttgcttggt actgtttctt     2040

ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag gtacagtagt tagttgaggt     2100

accggatcca cacgacacca tggctcatgc tgccctcagc cctctctccc aacgctttga     2160

gagaatagct gtccagccac tcactggtgt ccttggtgct gagatcactg gagtggactt     2220

gagggaacca cttgatgaca gcacctggaa tgagatattg gatgccttcc acacttacca     2280

agtcatctac tttcctggcc aagcaatcac caatgagcag cacattgcat tctcaagaag     2340

gtttggacca gttgatccag tgcctcttct caagagcatt gaaggctatc cagaggttca     2400

gatgatccgc agagaagcca atgagtctgg aagggtgatt ggtgatgact ggcacacaga     2460

ctccactttc cttgatgcac ctccagctgc tgttgtgatg agggccatag atgttcctga     2520

gcatggcgga gacactgggt tcctttcaat gtacacagct tgggagacct tgtctccaac     2580

catgcaagcc accatcgaag ggctcaacgt tgtgcactct gccacacgtg tgttcggttc     2640

cctctaccaa gcacagaacc gtcgcttcag caacacctca gtcaaggtga tggatgttga     2700

tgctggtgac agagagacag tccatccctt ggttgtgact catcctggct ctggaaggaa     2760

aggcctttat gtgaatcaag tctactgtca gagaattgag ggcatgacag atgcagaatc     2820

aaagccattg cttcagttcc tctatgagca tgccaccaga tttgacttca cttgccgtgt     2880

gaggtggaag aaagaccaag tccttgtctg ggacaacttg tgcaccatgc accgtgctgt     2940

tcctgactat gctggcaagt tcagatactt gactcgcacc acagttggtg gagttaggcc     3000

tgcccgctga gtagttagct taatcaccta gagctcggtc gcagcgtgtg cgtgtccgtc     3060

gtacgttctg gccggccggg ccttgggcgc gcgatcagaa gcgttgcgtt ggcgtgtgtg     3120

tgcttctggt ttgctttaat tttaccaagt ttgtttcaag gtggatcgcg tggtcaaggc     3180

ccgtgtgctt taaagaccca ccggcactgg cagtgagtgt tgctgcttgt gtaggctttg     3240

gtacgtatgg gctttatttg cttctggatg ttgtgtacta cttgggtttg ttgaattatt     3300

atgagcagtt gcgtattgta attcagctgg gctacctgga cattgttatg tattaataaa     3360

tgctttgctt tcttctaaag atctttaagt gctgaattca tatttcctcc tgcaggaaga     3420

ctcccgccca tctctctatg cccgggacaa gtg                                  3453


<210>  19
<211>  585
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TRAP4 and Cry34ab1 fusion gene

<400>  19
tcaggattta tggccgtagc ggctggaggt ggtctggata gtataggtca cgtgggattg       60

attgccgctg ccaccttggg tgatgatttc atactgggat ttattggagt ggccgtcgta      120

tttattgctg ccagcgaacg ggttgtcgaa gtacaggctg atctccgcct cgccgttgat      180

ggagtagtag atggtgccct cggtgccggt catgaagccg tttgattcgg ccacgaaggt      240

tttaatttgg tcgttcgcca cgttggtcgg gctggtcctc cagcggccac cgtcaagttt      300

cgttttatcc tccagctgca atgtatggcc agtcttatta ttcacgtcga tgtgcacctc      360

cctcgcggac atggagcaga cggttgtctg aagtctgcga gtcgagcgga tggatgactt      420

ggagcacgac ctcctcgcga cgctcagagg agccgaatag acatggggaa ccacgtcgtt      480

gacgcttatg ggtctcggaa caaccaacgc tcccacctcc acgcgtctcg cgaggccagc      540

gttacactga gaggctctca gtgatcctcc ttggcgagcc aacat                      585


<210>  20
<211>  570
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Trap8 and Cry34Ab1 fusion gene

<400>  20
atggctcaat ctagcagaat ctgccacggt gtgcagaacc catgtgtgat catttccaat       60

ctctccaaat ccaaccagaa caaatctcct ttctcagtca gcctcaagac tcaccagcag      120

cagcgtcgtg cttaccagat atctagctgg ggattgaaga agtcaaacaa cgggtccgtg      180

attcgtccgg ttaaggcaat gtccgcgagg gaggtgcaca tcgacgtgaa taataagact      240

ggccatacat tgcagctgga ggataaaacg aaacttgacg gtggccgctg gaggaccagc      300

ccgaccaacg tggcgaacga ccaaattaaa accttcgtgg ccgaatcaaa cggcttcatg      360

accggcaccg agggcaccat ctactactcc atcaacggcg aggcggagat cagcctgtac      420

ttcgacaacc cgttcgctgg cagcaataaa tacgacggcc actccaataa atcccagtat      480

gaaatcatca cccaaggtgg cagcggcaat caatcccacg tgacctatac tatccagacc      540

acctccagcc gctacggcca taaatcctga                                       570


<210>  21
<211>  594
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TRAP12 and cry34ab1 fusion gene

<400>  21
atggcgcagt cctctaggat ctgccacggc gtgcagaacc cgtgcgtgat catctcgaac       60

ctgtccaagt ccaaccagaa caagtccccg ttctccgtga gcctcaagac ccaccagcac      120

ccgagggcct acccgatcag cagctcctgg ggcctgaaga agagcggcat gaccctgatc      180

ggctccgagc tgaggccgct gaaggtgatg tcaagcgtga gcatgtccgc gagggaggtg      240

cacatcgacg tgaataataa gactggccat acattgcagc tggaggataa aacgaaactt      300

gacggtggcc gctggaggac cagcccgacc aacgtggcga acgaccaaat taaaaccttc      360

gtggccgaat caaacggctt catgaccggc accgagggca ccatctacta ctccatcaac      420

ggcgaggcgg agatcagcct gtacttcgac aacccgttcg ctggcagcaa taaatacgac      480

ggccactcca ataaatccca gtatgaaatc atcacccaag gtggcagcgg caatcaatcc      540

cacgtgacct atactatcca gaccacctcc agccgctacg gccataaatc ctga            594


<210>  22
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  eZFN4 binding site

<400>  22
gccttttgca gtttatctct atgcccggga caagtg                                 36


<210>  23
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  eZFN14 binding site

<400>  23
tggtcatcct catcctgtct atgcccggga caagtg                                 36


<210>  24
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer for AAD-12

<400>  24
aacccgtgct cttgttc                                                      17


<210>  25
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe for AAD-12

<400>  25
caggccgggt cagcct                                                       16


<210>  26
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer for AAD-12

<400>  26
ggatgcacct tgaccaat                                                     18


<210>  27
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer for YFP

<400>  27
cgtgttggga aagaacttgg a                                                 21


<210>  28
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe for YFP

<400>  28
cactccccac tgcct                                                        15


<210>  29
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer for YFP

<400>  29
ccgtggttgg cttggtct                                                     18


<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer for FokI

<400>  30
tgaatggtgg aaggtgtatc c                                                 21


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe for FokI

<400>  31
catctgttac agagttcaaa                                                   20


<210>  32
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer for FokI

<400>  32
aagctgtgct ttgtagttac cctta                                             25


<210>  33
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer for GMS116

<400>  33
gtaatatggg ctcagaggaa tggt                                              24


<210>  34
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe for GMS116

<400>  34
ccatggcccg gtaccatctg gtc                                               23


<210>  35
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer for GMS116

<400>  35
atggagaaga acattggaat tgc                                               23


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3176F forward primer for out-out PCR

<400>  36
attgagggga taaggccaac                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  9779R reverse primer for out-out PCR

<400>  37
tactgccgtg acgtagcatc                                                   20


