                         SEQUENCE LISTING

<110>  Danmarks Tekniske Universitet
 
<120>  Methods for producing fatty alcohols and derivatives thereof in 
       yeast

<130>  P4260PC00

<160>  114   

<170>  PatentIn version 3.5

<210>  1
<211>  981
<212>  DNA
<213>  Artificial sequence

<220>
<223>  codon-optimized nucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(981)
<223>  S. cerevisiae-codon-optimized nucleotide sequence of A. 
       transitella delta11-desaturase; mRNA-coding sequence.

<400>  1
atggttccaa acaagggttc ctctgatgtt ttgtctgaac attctgaacc acaattcacc       60

aagttgattg ctccacaagc tggtccaaga aagtacaaaa tcgtttacag aaacttgttg      120

accttcggtt actggcattt gtctgctgtt tatggtttgt acttgtgttt cacttgtgct      180

aagtgggcta ctattttgtt cgctttcttc ttgtacgtta tcgccgaaat tggtattact      240

ggtggtgctc atagattatg ggctcataga acttacaaag ccaagttgcc attggaaatc      300

ttgttgttga tcatgaactc cattgccttc caagatactg cttttacttg ggctagagat      360

catagattgc atcacaagta ctctgatact gatgctgatc cacataatgc tactagaggt      420

ttcttctact ctcatgttgg ttggttgttg gttaagaaac acccagaagt taaggctaga      480

ggtaagtact tgtctttgga tgacttgaag aacaaccctt tgttgaagtt ccaaaagaag      540

tacgccattt tggtcattgg tactttgtgc tttttgatgc caactttcgt tccagtttac      600

ttttggggtg aaggtatttc tactgcctgg aacattaact tgttaagata cgtcatgaac      660

ttgaacatga cctttttggt taactccgct gctcatattt ttggtaacaa gccatacgat      720

aagtctatcg cctctgttca aaacatctct gtttctttgg ctactttcgg tgaaggtttc      780

cataactacc atcatactta tccatgggat tacagagctg ctgaattggg taacaataga      840

ttgaatatga ccaccgcctt cattgatttc tttgcttgga ttggttgggc ctacgatttg      900

aaatctgttc cacaagaagc tattgctaag agatgtgcta aaactggtga tggtactgat      960

atgtggggta gaaagagatg a                                                981


<210>  2
<211>  326
<212>  PRT
<213>  Amyelois transitella


<220>
<221>  MISC_FEATURE
<222>  (1)..(326)
<223>  Amino acid sequence of A. transitella delta-11-desaturase 
       (translation)

<400>  2

Met Val Pro Asn Lys Gly Ser Ser Asp Val Leu Ser Glu His Ser Glu 
1               5                   10                  15      


Pro Gln Phe Thr Lys Leu Ile Ala Pro Gln Ala Gly Pro Arg Lys Tyr 
            20                  25                  30          


Lys Ile Val Tyr Arg Asn Leu Leu Thr Phe Gly Tyr Trp His Leu Ser 
        35                  40                  45              


Ala Val Tyr Gly Leu Tyr Leu Cys Phe Thr Cys Ala Lys Trp Ala Thr 
    50                  55                  60                  


Ile Leu Phe Ala Phe Phe Leu Tyr Val Ile Ala Glu Ile Gly Ile Thr 
65                  70                  75                  80  


Gly Gly Ala His Arg Leu Trp Ala His Arg Thr Tyr Lys Ala Lys Leu 
                85                  90                  95      


Pro Leu Glu Ile Leu Leu Leu Ile Met Asn Ser Ile Ala Phe Gln Asp 
            100                 105                 110         


Thr Ala Phe Thr Trp Ala Arg Asp His Arg Leu His His Lys Tyr Ser 
        115                 120                 125             


Asp Thr Asp Ala Asp Pro His Asn Ala Thr Arg Gly Phe Phe Tyr Ser 
    130                 135                 140                 


His Val Gly Trp Leu Leu Val Lys Lys His Pro Glu Val Lys Ala Arg 
145                 150                 155                 160 


Gly Lys Tyr Leu Ser Leu Asp Asp Leu Lys Asn Asn Pro Leu Leu Lys 
                165                 170                 175     


Phe Gln Lys Lys Tyr Ala Ile Leu Val Ile Gly Thr Leu Cys Phe Leu 
            180                 185                 190         


Met Pro Thr Phe Val Pro Val Tyr Phe Trp Gly Glu Gly Ile Ser Thr 
        195                 200                 205             


Ala Trp Asn Ile Asn Leu Leu Arg Tyr Val Met Asn Leu Asn Met Thr 
    210                 215                 220                 


Phe Leu Val Asn Ser Ala Ala His Ile Phe Gly Asn Lys Pro Tyr Asp 
225                 230                 235                 240 


Lys Ser Ile Ala Ser Val Gln Asn Ile Ser Val Ser Leu Ala Thr Phe 
                245                 250                 255     


Gly Glu Gly Phe His Asn Tyr His His Thr Tyr Pro Trp Asp Tyr Arg 
            260                 265                 270         


Ala Ala Glu Leu Gly Asn Asn Arg Leu Asn Met Thr Thr Ala Phe Ile 
        275                 280                 285             


Asp Phe Phe Ala Trp Ile Gly Trp Ala Tyr Asp Leu Lys Ser Val Pro 
    290                 295                 300                 


Gln Glu Ala Ile Ala Lys Arg Cys Ala Lys Thr Gly Asp Gly Thr Asp 
305                 310                 315                 320 


Met Trp Gly Arg Lys Arg 
                325     


<210>  3
<211>  1560
<212>  DNA
<213>  Yarrowia lipolytica

<400>  3
atgtctacct ttgattggga atccattgtg cctgccactc ctctcgacca gattcctggc       60

gacatccagc gactgcgaaa gggcttccga tccggaaaga ccctcgatct caactaccga      120

ctggaccaga ttcgaaactt gcactacgtc ctcagagaca atgtcgaggc catcaaggac      180

gccgtgtaca aggatctcgg ccgacccaag cacgagactg acctgtgcga ggtgggtttc      240

ctgtggggcg agtttaacaa cgtggttgcc aacctcaaga agtgggccgc cgacgaggac      300

gtcaagacca acctgcagta ctccatctcc tcccccaaga tccgaaagcg acctcttgga      360

aacgtgctca tcatctcgcc ctggaactac ccctttatgc tgaccgtgtc tcctctcatt      420

ggagctctgg ctgccggtaa cactgtggct gtcaagttct ccgaaatggc cccccacact      480

tccaaaattg ttggcgactt gtgcaccaag gccctcgacc ccgacgtctt ccaggccatc      540

cagggaggtg tccccgtcgt caccaagacc ctcgagcaga agttcgacaa gattatgtac      600

actggtaacc acactgtcgg taagatcatt gccactgccg ccaacaagta cctgacaccc      660

gtcatcctcg agctcggagg taagtcgccc gtttttgtca ccaagaactg caagaacatc      720

aagcttgccg ctaagcgagc cctgtggggt aaggtggtaa acgctggcca gacctgtgtg      780

gctcccgact acgtgattgt cgagcccgag gtggagcagg agtttatcga cgcctgcaag      840

tactggatta acgagttcta cagtggtaag attgaccagt acaaccccga ctttgccaag      900

atcgccaccc ccaaccactg gaaccgactt acctccatgt tgagcaagtc caagggagag      960

atcattactg gaggtaacac tgacgagaag actcgattca tcgctcctac tgtcgtcgca     1020

aaggtccccg acaatgattc cctgatggag gacgagattt tcggccctct tctgcccatt     1080

ctcactgccc gatccgtcga ggagggtatc aagtacgtgc acgagaacca cgacacccct     1140

cttgccatgt acgtcttcac tgacaaggcc tctgagggcg actacatcca gtcccagatc     1200

aactctggtg gccttatctt caatgacact ctgatccacg ttggatgtgt ccaggctccg     1260

tttggtggtg tcggcatgtc cggttacggt gcttaccatg gcgaggactc cttcctggcc     1320

ttcacccacc gacaaaccta cctcaaccag cccaagcttc tggagcctct tcaggacgtg     1380

cgatacgccc cctacaccaa aaccaagcga agcatggtca agaacctgct gctggtcggc     1440

cccattttcc cccgaaccgg ctccgtatac cccaacgtgc tgatccgaat cttccgaaag     1500

atttggttct gggtccttat tgtcgccatc ggagctgctg gtgccaaggc tctgctctag     1560


<210>  4
<211>  519
<212>  PRT
<213>  Yarrowia lipolytica

<400>  4

Met Ser Thr Phe Asp Trp Glu Ser Ile Val Pro Ala Thr Pro Leu Asp 
1               5                   10                  15      


Gln Ile Pro Gly Asp Ile Gln Arg Leu Arg Lys Gly Phe Arg Ser Gly 
            20                  25                  30          


Lys Thr Leu Asp Leu Asn Tyr Arg Leu Asp Gln Ile Arg Asn Leu His 
        35                  40                  45              


Tyr Val Leu Arg Asp Asn Val Glu Ala Ile Lys Asp Ala Val Tyr Lys 
    50                  55                  60                  


Asp Leu Gly Arg Pro Lys His Glu Thr Asp Leu Cys Glu Val Gly Phe 
65                  70                  75                  80  


Leu Trp Gly Glu Phe Asn Asn Val Val Ala Asn Leu Lys Lys Trp Ala 
                85                  90                  95      


Ala Asp Glu Asp Val Lys Thr Asn Leu Gln Tyr Ser Ile Ser Ser Pro 
            100                 105                 110         


Lys Ile Arg Lys Arg Pro Leu Gly Asn Val Leu Ile Ile Ser Pro Trp 
        115                 120                 125             


Asn Tyr Pro Phe Met Leu Thr Val Ser Pro Leu Ile Gly Ala Leu Ala 
    130                 135                 140                 


Ala Gly Asn Thr Val Ala Val Lys Phe Ser Glu Met Ala Pro His Thr 
145                 150                 155                 160 


Ser Lys Ile Val Gly Asp Leu Cys Thr Lys Ala Leu Asp Pro Asp Val 
                165                 170                 175     


Phe Gln Ala Ile Gln Gly Gly Val Pro Val Val Thr Lys Thr Leu Glu 
            180                 185                 190         


Gln Lys Phe Asp Lys Ile Met Tyr Thr Gly Asn His Thr Val Gly Lys 
        195                 200                 205             


Ile Ile Ala Thr Ala Ala Asn Lys Tyr Leu Thr Pro Val Ile Leu Glu 
    210                 215                 220                 


Leu Gly Gly Lys Ser Pro Val Phe Val Thr Lys Asn Cys Lys Asn Ile 
225                 230                 235                 240 


Lys Leu Ala Ala Lys Arg Ala Leu Trp Gly Lys Val Val Asn Ala Gly 
                245                 250                 255     


Gln Thr Cys Val Ala Pro Asp Tyr Val Ile Val Glu Pro Glu Val Glu 
            260                 265                 270         


Gln Glu Phe Ile Asp Ala Cys Lys Tyr Trp Ile Asn Glu Phe Tyr Ser 
        275                 280                 285             


Gly Lys Ile Asp Gln Tyr Asn Pro Asp Phe Ala Lys Ile Ala Thr Pro 
    290                 295                 300                 


Asn His Trp Asn Arg Leu Thr Ser Met Leu Ser Lys Ser Lys Gly Glu 
305                 310                 315                 320 


Ile Ile Thr Gly Gly Asn Thr Asp Glu Lys Thr Arg Phe Ile Ala Pro 
                325                 330                 335     


Thr Val Val Ala Lys Val Pro Asp Asn Asp Ser Leu Met Glu Asp Glu 
            340                 345                 350         


Ile Phe Gly Pro Leu Leu Pro Ile Leu Thr Ala Arg Ser Val Glu Glu 
        355                 360                 365             


Gly Ile Lys Tyr Val His Glu Asn His Asp Thr Pro Leu Ala Met Tyr 
    370                 375                 380                 


Val Phe Thr Asp Lys Ala Ser Glu Gly Asp Tyr Ile Gln Ser Gln Ile 
385                 390                 395                 400 


Asn Ser Gly Gly Leu Ile Phe Asn Asp Thr Leu Ile His Val Gly Cys 
                405                 410                 415     


Val Gln Ala Pro Phe Gly Gly Val Gly Met Ser Gly Tyr Gly Ala Tyr 
            420                 425                 430         


His Gly Glu Asp Ser Phe Leu Ala Phe Thr His Arg Gln Thr Tyr Leu 
        435                 440                 445             


Asn Gln Pro Lys Leu Leu Glu Pro Leu Gln Asp Val Arg Tyr Ala Pro 
    450                 455                 460                 


Tyr Thr Lys Thr Lys Arg Ser Met Val Lys Asn Leu Leu Leu Val Gly 
465                 470                 475                 480 


Pro Ile Phe Pro Arg Thr Gly Ser Val Tyr Pro Asn Val Leu Ile Arg 
                485                 490                 495     


Ile Phe Arg Lys Ile Trp Phe Trp Val Leu Ile Val Ala Ile Gly Ala 
            500                 505                 510         


Ala Gly Ala Lys Ala Leu Leu 
        515                 


<210>  5
<211>  1590
<212>  DNA
<213>  Yarrowia lipolytica

<400>  5
atgactacca ctgccacaga gacccccacg acaaacgtga cccccaccac gtcactgccc       60

aaggagaccg cctccccagg agggaccgct tctgtcaaca cgtcattcga ctgggagagc      120

atctgcggca agacgccgtt ggaggagatc gagtcggaca tttcgcgtct caaaaagacc      180

ttccgatcgg gcaaaactct ggatctggac taccgactcg accagatccg aaacctggcg      240

tatgcgatcc gcgataacga aaacaagatc cgcgacgcca tcaaggcgga cctgaaacga      300

cctgacttcg aaaccatggc ggccgagttc tcggtccaga tgggcgaatt caactacgtg      360

gtcaaaaacc tgccgaaatg ggtcaaggac gaaaaagtca agggaaccag catggcgtac      420

tggaactcgt cgccaaagat ccggaaacgg cccctgggct ccgtgcttgt catcacgccc      480

tggaactacc cactgattct ggccgtgtcg cctgttctgg gcgccattgc cgcaggcaac      540

accgtggcgc tgaaaatgtc agaaatgtca cccaacgcgt caaaggtgat tggcgacatt      600

atgacagctg ccctggaccc ccagctcttt caatgcttct tcggaggagt ccccgaaacc      660

accgagatcc tcaaacacag atgggacaag atcatgtaca ccggaaacgg caaagtgggc      720

cgaatcatct gtgaggctgc caacaagtac ttgacacctg tggagctcga actcggagga      780

aagtcgcctg ttttcgtcac caaacactgc tccaacctgg aaatggccgc ccgccgaatc      840

atctggggca aattcgtcaa cggaggacaa acctgcgtgg ctccagacta cgttctggtg      900

tgtcccgagg tccacgacaa atttgtggct gcctgtcaaa aggtgctgga caagttctac      960

cctaacaact ctgccgagtc cgagatggcc catatcgcca cccctctcca ttacgagcgt     1020

ttgacgggcc tgctcaattc cacccgaggt aaggtcgttg ctggaggcac tttcaactcg     1080

gccacccggt tcattgctcc tacgattgtc gacggagtgg atgccaacga ttctctgatg     1140

cagggagaac tgtttggtcc tcttctcccc attgtcaagg ccatgagcac cgaggctgcc     1200

tgcaactttg tgcttgagca ccaccccacc cccctggcag agtacatctt ttcagataac     1260

aattctgaga ttgattacat ccgagatcga gtgtcgtctg gaggtctcgt gatcaacgac     1320

actctgatcc acgtgggatg cgtacaggcg ccctttggag gtgtcggaga cagtggaaat     1380

ggaggatacc atggcaagca cactttcgat ttgttcagcc attctcagac ggtcctcaga     1440

caacccggat gggtcgaaat gctgcagaag aaacggtatc ctccgtacaa caagagcaac     1500

gagaagtttg tccggagaat ggtggtcccc agccctggtt ttccccggga gggtgacgtg     1560

agaggatttt ggtcgagact cttcaactag                                      1590


<210>  6
<211>  529
<212>  PRT
<213>  Yarrowia lipolytica

<400>  6

Met Thr Thr Thr Ala Thr Glu Thr Pro Thr Thr Asn Val Thr Pro Thr 
1               5                   10                  15      


Thr Ser Leu Pro Lys Glu Thr Ala Ser Pro Gly Gly Thr Ala Ser Val 
            20                  25                  30          


Asn Thr Ser Phe Asp Trp Glu Ser Ile Cys Gly Lys Thr Pro Leu Glu 
        35                  40                  45              


Glu Ile Glu Ser Asp Ile Ser Arg Leu Lys Lys Thr Phe Arg Ser Gly 
    50                  55                  60                  


Lys Thr Leu Asp Leu Asp Tyr Arg Leu Asp Gln Ile Arg Asn Leu Ala 
65                  70                  75                  80  


Tyr Ala Ile Arg Asp Asn Glu Asn Lys Ile Arg Asp Ala Ile Lys Ala 
                85                  90                  95      


Asp Leu Lys Arg Pro Asp Phe Glu Thr Met Ala Ala Glu Phe Ser Val 
            100                 105                 110         


Gln Met Gly Glu Phe Asn Tyr Val Val Lys Asn Leu Pro Lys Trp Val 
        115                 120                 125             


Lys Asp Glu Lys Val Lys Gly Thr Ser Met Ala Tyr Trp Asn Ser Ser 
    130                 135                 140                 


Pro Lys Ile Arg Lys Arg Pro Leu Gly Ser Val Leu Val Ile Thr Pro 
145                 150                 155                 160 


Trp Asn Tyr Pro Leu Ile Leu Ala Val Ser Pro Val Leu Gly Ala Ile 
                165                 170                 175     


Ala Ala Gly Asn Thr Val Ala Leu Lys Met Ser Glu Met Ser Pro Asn 
            180                 185                 190         


Ala Ser Lys Val Ile Gly Asp Ile Met Thr Ala Ala Leu Asp Pro Gln 
        195                 200                 205             


Leu Phe Gln Cys Phe Phe Gly Gly Val Pro Glu Thr Thr Glu Ile Leu 
    210                 215                 220                 


Lys His Arg Trp Asp Lys Ile Met Tyr Thr Gly Asn Gly Lys Val Gly 
225                 230                 235                 240 


Arg Ile Ile Cys Glu Ala Ala Asn Lys Tyr Leu Thr Pro Val Glu Leu 
                245                 250                 255     


Glu Leu Gly Gly Lys Ser Pro Val Phe Val Thr Lys His Cys Ser Asn 
            260                 265                 270         


Leu Glu Met Ala Ala Arg Arg Ile Ile Trp Gly Lys Phe Val Asn Gly 
        275                 280                 285             


Gly Gln Thr Cys Val Ala Pro Asp Tyr Val Leu Val Cys Pro Glu Val 
    290                 295                 300                 


His Asp Lys Phe Val Ala Ala Cys Gln Lys Val Leu Asp Lys Phe Tyr 
305                 310                 315                 320 


Pro Asn Asn Ser Ala Glu Ser Glu Met Ala His Ile Ala Thr Pro Leu 
                325                 330                 335     


His Tyr Glu Arg Leu Thr Gly Leu Leu Asn Ser Thr Arg Gly Lys Val 
            340                 345                 350         


Val Ala Gly Gly Thr Phe Asn Ser Ala Thr Arg Phe Ile Ala Pro Thr 
        355                 360                 365             


Ile Val Asp Gly Val Asp Ala Asn Asp Ser Leu Met Gln Gly Glu Leu 
    370                 375                 380                 


Phe Gly Pro Leu Leu Pro Ile Val Lys Ala Met Ser Thr Glu Ala Ala 
385                 390                 395                 400 


Cys Asn Phe Val Leu Glu His His Pro Thr Pro Leu Ala Glu Tyr Ile 
                405                 410                 415     


Phe Ser Asp Asn Asn Ser Glu Ile Asp Tyr Ile Arg Asp Arg Val Ser 
            420                 425                 430         


Ser Gly Gly Leu Val Ile Asn Asp Thr Leu Ile His Val Gly Cys Val 
        435                 440                 445             


Gln Ala Pro Phe Gly Gly Val Gly Asp Ser Gly Asn Gly Gly Tyr His 
    450                 455                 460                 


Gly Lys His Thr Phe Asp Leu Phe Ser His Ser Gln Thr Val Leu Arg 
465                 470                 475                 480 


Gln Pro Gly Trp Val Glu Met Leu Gln Lys Lys Arg Tyr Pro Pro Tyr 
                485                 490                 495     


Asn Lys Ser Asn Glu Lys Phe Val Arg Arg Met Val Val Pro Ser Pro 
            500                 505                 510         


Gly Phe Pro Arg Glu Gly Asp Val Arg Gly Phe Trp Ser Arg Leu Phe 
        515                 520                 525             


Asn 
    


<210>  7
<211>  1134
<212>  DNA
<213>  Yarrowia lipolytica

<400>  7
atgtggggaa gttcacatgc attcgctggt gaatctgatc tgacactaca actacacacc       60

aggtccaaca tgagcgacaa tacgacaatc aaaaagccga tccgacccaa accgatccgg      120

acggaacgcc tgccttacgc tggggccgca gaaatcatcc gagccaacca gaaagaccac      180

tactttgagt ccgtgcttga acagcatctc gtcacgtttc tgcagaaatg gaagggagta      240

cgatttatcc accagtacaa ggaggagctg gagacggcgt ccaagtttgc atatctcggt      300

ttgtgtacgc ttgtgggctc caagactctc ggagaagagt acaccaatct catgtacact      360

atcagagacc gaacagctct accgggggtg gtgagacggt ttggctacgt gctttccaac      420

actctgtttc catacctgtt tgtgcgctac atgggcaagt tgcgcgccaa actgatgcgc      480

gagtatcccc atctggtgga gtacgacgaa gatgagcctg tgcccagccc ggaaacatgg      540

aaggagcggg tcatcaagac gtttgtgaac aagtttgaca agttcacggc gctggagggg      600

tttaccgcga tccacttggc gattttctac gtctacggct cgtactacca gctcagtaag      660

cggatctggg gcatgcgtta tgtatttgga caccgactgg acaagaatga gcctcgaatc      720

ggttacgaga tgctcggtct gctgattttc gcccggtttg ccacgtcatt tgtgcagacg      780

ggaagagagt acctcggagc gctgctggaa aagagcgtgg agaaagaggc aggggagaag      840

gaagatgaaa aggaagcggt tgtgccgaaa aagaagtcgt caattccgtt cattgaggat      900

acagaagggg agacggaaga caagatcgat ctggaggacc ctcgacagct caagttcatt      960

cctgaggcgt ccagagcgtg cactctgtgt ctgtcataca ttagtgcgcc ggcatgtacg     1020

ccatgtggac actttttctg ttgggactgt atttccgaat gggtgagaga gaagcccgag     1080

tgtcccttgt gtcggcaggg tgtgagagag cagaacttgt tgcctatcag ataa           1134


<210>  8
<211>  377
<212>  PRT
<213>  Yarrowia lipolytica

<400>  8

Met Trp Gly Ser Ser His Ala Phe Ala Gly Glu Ser Asp Leu Thr Leu 
1               5                   10                  15      


Gln Leu His Thr Arg Ser Asn Met Ser Asp Asn Thr Thr Ile Lys Lys 
            20                  25                  30          


Pro Ile Arg Pro Lys Pro Ile Arg Thr Glu Arg Leu Pro Tyr Ala Gly 
        35                  40                  45              


Ala Ala Glu Ile Ile Arg Ala Asn Gln Lys Asp His Tyr Phe Glu Ser 
    50                  55                  60                  


Val Leu Glu Gln His Leu Val Thr Phe Leu Gln Lys Trp Lys Gly Val 
65                  70                  75                  80  


Arg Phe Ile His Gln Tyr Lys Glu Glu Leu Glu Thr Ala Ser Lys Phe 
                85                  90                  95      


Ala Tyr Leu Gly Leu Cys Thr Leu Val Gly Ser Lys Thr Leu Gly Glu 
            100                 105                 110         


Glu Tyr Thr Asn Leu Met Tyr Thr Ile Arg Asp Arg Thr Ala Leu Pro 
        115                 120                 125             


Gly Val Val Arg Arg Phe Gly Tyr Val Leu Ser Asn Thr Leu Phe Pro 
    130                 135                 140                 


Tyr Leu Phe Val Arg Tyr Met Gly Lys Leu Arg Ala Lys Leu Met Arg 
145                 150                 155                 160 


Glu Tyr Pro His Leu Val Glu Tyr Asp Glu Asp Glu Pro Val Pro Ser 
                165                 170                 175     


Pro Glu Thr Trp Lys Glu Arg Val Ile Lys Thr Phe Val Asn Lys Phe 
            180                 185                 190         


Asp Lys Phe Thr Ala Leu Glu Gly Phe Thr Ala Ile His Leu Ala Ile 
        195                 200                 205             


Phe Tyr Val Tyr Gly Ser Tyr Tyr Gln Leu Ser Lys Arg Ile Trp Gly 
    210                 215                 220                 


Met Arg Tyr Val Phe Gly His Arg Leu Asp Lys Asn Glu Pro Arg Ile 
225                 230                 235                 240 


Gly Tyr Glu Met Leu Gly Leu Leu Ile Phe Ala Arg Phe Ala Thr Ser 
                245                 250                 255     


Phe Val Gln Thr Gly Arg Glu Tyr Leu Gly Ala Leu Leu Glu Lys Ser 
            260                 265                 270         


Val Glu Lys Glu Ala Gly Glu Lys Glu Asp Glu Lys Glu Ala Val Val 
        275                 280                 285             


Pro Lys Lys Lys Ser Ser Ile Pro Phe Ile Glu Asp Thr Glu Gly Glu 
    290                 295                 300                 


Thr Glu Asp Lys Ile Asp Leu Glu Asp Pro Arg Gln Leu Lys Phe Ile 
305                 310                 315                 320 


Pro Glu Ala Ser Arg Ala Cys Thr Leu Cys Leu Ser Tyr Ile Ser Ala 
                325                 330                 335     


Pro Ala Cys Thr Pro Cys Gly His Phe Phe Cys Trp Asp Cys Ile Ser 
            340                 345                 350         


Glu Trp Val Arg Glu Lys Pro Glu Cys Pro Leu Cys Arg Gln Gly Val 
        355                 360                 365             


Arg Glu Gln Asn Leu Leu Pro Ile Arg 
    370                 375         


<210>  9
<211>  2184
<212>  DNA
<213>  Yarrowia lipolytica

<400>  9
atgtccgaaa ccgaccatct gctggccgcc gagcccgtgg ctgagtaccc ccagtacacg       60

ccttggccca actcccgaaa atcagtggac acggagtttt ccgcaacctc gtggatttac      120

gacttggttc tgtggatttt cacggcttgc tttgacattt ttttcagaga aatccggcca      180

cgtggtgcct tccgaatccc cagaaagggc cccgtgctgt tcgtggctgc cccccacgca      240

aaccagtttg tggaccccgt catcctcatg aaccaggtca aacaggaggc cggacgacga      300

atctccttcc ttgtggccga gaagtccatg cgacgagctg cagtcggacg aatggcccga      360

agcatgaact caattcctgt cgtgcgagct caggacaatg caaaaaaggg agagggaaag      420

atttacgtcg acgcagagga ccccacaaag atccacggaa tcggcaccca gttcacgaag      480

cagtgcgagg tgcgaggcct cgtggtctgc tcgtcctctg tcggctcaat tgacgtggct      540

gagattgtgt ccgacactct gctcattgca agaaaggaat tcaagggccc caaagccaag      600

gaggctctca aggaatccaa cggaggaatc acatacaagt acgccgacta cgtcaaccag      660

gccacagtct accgatccgt attcgacaaa ttgcaccatg gaggctgtgt gggtatcttc      720

ccagagggag gatctcatga ccgaaccgaa ctgctgcccc ttaaggccgg tgttgctatc      780

atggctctgg gggctctcgc agaggacccc tcttgtggtg tgcgaatcgt cccctgtggt      840

ctcaactact tccacgccca caagttccga tctcgggccg tggtggagtt tggctctcct      900

attgccattc ctccggatct cgtggagaag tacaaggcag gaggagaggc caagcgggag      960

gctgtcaaga ccgttctaga cattactgcc gctggtctca agtctgtgac tgttcaggtg     1020

caggatttcg acaccctgat gctgatccag gccattcgac gactctaccg acctcccgga     1080

aagaagattc ctctgcccat ggttgtagag ctcaaccgtc gacttgtata cgcctacaac     1140

cactacaagg acgatccccg tatcgaggag atgaagcagg agattcgaaa gtacaacaag     1200

ttcctgcagg ccatgggtct caaggaccat caggtagaga aggcccgaat ctccaagatt     1260

gagattctgg gccggcttct gtaccggtcc atcaagcttg tgttcttgtc cattggctgt     1320

ctccccggtc tgcttttgtt ttctcccatc ttcatcattt ctaagtccat ttccaaaacc     1380

aaggccaagg aggctctcaa ggcctccagt gtcaaaatca aggctaacga tgtggttgcc     1440

acttggaagg tgctggttgc aatgggtctg accccagttc tttacattct ctattcactg     1500

gttggatctg tggtgattcg aaagctcgat ctcatctcct ggttccccac aattcttctt     1560

cccggcctcg ttttaagcat catcatcaca acctcatacg ccgccctggc tatgggagag     1620

gccggtatgg acattttcaa gtctcttcga ccacttgcat tggctctcaa cccttccacc     1680

aaaaactctc tgctcaagct gcaaaatgaa cgaaagcgac ttgtgctcaa gtcttccgag     1740

ctcgttacct ctttgggccc tgagctgttc cccgacttcc ccgagaactc cattctgcag     1800

ggaagcgata agtttgagga cgaggagaac tacgaaaacg agaagcgatc gcattccaga     1860

tccacttctg ccacttctct atctgccatg agcgagggag acggtgatga gcttgttcgg     1920

gaggtccgaa agggtgctag ctacttccct gtgagtacca tttctgagga cgaagaccaa     1980

gccatctcgc gagtgggctc tgaggcatct cttgctgaca ttcctctgtt tggtatgtcc     2040

cgatcacaat ctggagcttc tctttcggaa gcctccacac acggctcttc tactggagct     2100

gatgccgagg aggctaagac ggaggtgact cgcagaattg cattggcgat ggaggaaaaa     2160

cgacgagagc aggatgagga ataa                                            2184


<210>  10
<211>  727
<212>  PRT
<213>  Yarrowia lipolytica

<400>  10

Met Ser Glu Thr Asp His Leu Leu Ala Ala Glu Pro Val Ala Glu Tyr 
1               5                   10                  15      


Pro Gln Tyr Thr Pro Trp Pro Asn Ser Arg Lys Ser Val Asp Thr Glu 
            20                  25                  30          


Phe Ser Ala Thr Ser Trp Ile Tyr Asp Leu Val Leu Trp Ile Phe Thr 
        35                  40                  45              


Ala Cys Phe Asp Ile Phe Phe Arg Glu Ile Arg Pro Arg Gly Ala Phe 
    50                  55                  60                  


Arg Ile Pro Arg Lys Gly Pro Val Leu Phe Val Ala Ala Pro His Ala 
65                  70                  75                  80  


Asn Gln Phe Val Asp Pro Val Ile Leu Met Asn Gln Val Lys Gln Glu 
                85                  90                  95      


Ala Gly Arg Arg Ile Ser Phe Leu Val Ala Glu Lys Ser Met Arg Arg 
            100                 105                 110         


Ala Ala Val Gly Arg Met Ala Arg Ser Met Asn Ser Ile Pro Val Val 
        115                 120                 125             


Arg Ala Gln Asp Asn Ala Lys Lys Gly Glu Gly Lys Ile Tyr Val Asp 
    130                 135                 140                 


Ala Glu Asp Pro Thr Lys Ile His Gly Ile Gly Thr Gln Phe Thr Lys 
145                 150                 155                 160 


Gln Cys Glu Val Arg Gly Leu Val Val Cys Ser Ser Ser Val Gly Ser 
                165                 170                 175     


Ile Asp Val Ala Glu Ile Val Ser Asp Thr Leu Leu Ile Ala Arg Lys 
            180                 185                 190         


Glu Phe Lys Gly Pro Lys Ala Lys Glu Ala Leu Lys Glu Ser Asn Gly 
        195                 200                 205             


Gly Ile Thr Tyr Lys Tyr Ala Asp Tyr Val Asn Gln Ala Thr Val Tyr 
    210                 215                 220                 


Arg Ser Val Phe Asp Lys Leu His His Gly Gly Cys Val Gly Ile Phe 
225                 230                 235                 240 


Pro Glu Gly Gly Ser His Asp Arg Thr Glu Leu Leu Pro Leu Lys Ala 
                245                 250                 255     


Gly Val Ala Ile Met Ala Leu Gly Ala Leu Ala Glu Asp Pro Ser Cys 
            260                 265                 270         


Gly Val Arg Ile Val Pro Cys Gly Leu Asn Tyr Phe His Ala His Lys 
        275                 280                 285             


Phe Arg Ser Arg Ala Val Val Glu Phe Gly Ser Pro Ile Ala Ile Pro 
    290                 295                 300                 


Pro Asp Leu Val Glu Lys Tyr Lys Ala Gly Gly Glu Ala Lys Arg Glu 
305                 310                 315                 320 


Ala Val Lys Thr Val Leu Asp Ile Thr Ala Ala Gly Leu Lys Ser Val 
                325                 330                 335     


Thr Val Gln Val Gln Asp Phe Asp Thr Leu Met Leu Ile Gln Ala Ile 
            340                 345                 350         


Arg Arg Leu Tyr Arg Pro Pro Gly Lys Lys Ile Pro Leu Pro Met Val 
        355                 360                 365             


Val Glu Leu Asn Arg Arg Leu Val Tyr Ala Tyr Asn His Tyr Lys Asp 
    370                 375                 380                 


Asp Pro Arg Ile Glu Glu Met Lys Gln Glu Ile Arg Lys Tyr Asn Lys 
385                 390                 395                 400 


Phe Leu Gln Ala Met Gly Leu Lys Asp His Gln Val Glu Lys Ala Arg 
                405                 410                 415     


Ile Ser Lys Ile Glu Ile Leu Gly Arg Leu Leu Tyr Arg Ser Ile Lys 
            420                 425                 430         


Leu Val Phe Leu Ser Ile Gly Cys Leu Pro Gly Leu Leu Leu Phe Ser 
        435                 440                 445             


Pro Ile Phe Ile Ile Ser Lys Ser Ile Ser Lys Thr Lys Ala Lys Glu 
    450                 455                 460                 


Ala Leu Lys Ala Ser Ser Val Lys Ile Lys Ala Asn Asp Val Val Ala 
465                 470                 475                 480 


Thr Trp Lys Val Leu Val Ala Met Gly Leu Thr Pro Val Leu Tyr Ile 
                485                 490                 495     


Leu Tyr Ser Leu Val Gly Ser Val Val Ile Arg Lys Leu Asp Leu Ile 
            500                 505                 510         


Ser Trp Phe Pro Thr Ile Leu Leu Pro Gly Leu Val Leu Ser Ile Ile 
        515                 520                 525             


Ile Thr Thr Ser Tyr Ala Ala Leu Ala Met Gly Glu Ala Gly Met Asp 
    530                 535                 540                 


Ile Phe Lys Ser Leu Arg Pro Leu Ala Leu Ala Leu Asn Pro Ser Thr 
545                 550                 555                 560 


Lys Asn Ser Leu Leu Lys Leu Gln Asn Glu Arg Lys Arg Leu Val Leu 
                565                 570                 575     


Lys Ser Ser Glu Leu Val Thr Ser Leu Gly Pro Glu Leu Phe Pro Asp 
            580                 585                 590         


Phe Pro Glu Asn Ser Ile Leu Gln Gly Ser Asp Lys Phe Glu Asp Glu 
        595                 600                 605             


Glu Asn Tyr Glu Asn Glu Lys Arg Ser His Ser Arg Ser Thr Ser Ala 
    610                 615                 620                 


Thr Ser Leu Ser Ala Met Ser Glu Gly Asp Gly Asp Glu Leu Val Arg 
625                 630                 635                 640 


Glu Val Arg Lys Gly Ala Ser Tyr Phe Pro Val Ser Thr Ile Ser Glu 
                645                 650                 655     


Asp Glu Asp Gln Ala Ile Ser Arg Val Gly Ser Glu Ala Ser Leu Ala 
            660                 665                 670         


Asp Ile Pro Leu Phe Gly Met Ser Arg Ser Gln Ser Gly Ala Ser Leu 
        675                 680                 685             


Ser Glu Ala Ser Thr His Gly Ser Ser Thr Gly Ala Asp Ala Glu Glu 
    690                 695                 700                 


Ala Lys Thr Glu Val Thr Arg Arg Ile Ala Leu Ala Met Glu Glu Lys 
705                 710                 715                 720 


Arg Arg Glu Gln Asp Glu Glu 
                725         


<210>  11
<211>  1362
<212>  DNA
<213>  Artificial sequence

<220>
<223>  codon-optimized nucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(1362)
<223>  S. cerevisiae-codon-optimized nucleotide sequence of H. subflexa 
       fatty acyl reductase; mRNA-coding sequence.

<400>  11
atggttgtct tgacctccaa agaaactaag ccatctgttg ctgaatttta cgctggtaag       60

tctgttttca ttactggtgg tactggtttc ttgggtaagg ttttcattga aaagttgttg      120

tactcctgcc cagatatcgg taatatctac atgttgatca gagaaaagaa gggtttgtcc      180

gtttccgaaa gaatcaagca ctttttggat gatcctttgt tcaccagatt gaaagaaaaa      240

agaccagccg acttggaaaa gatcgttttg attccaggtg atattactgc tccagatttg      300

ggtattacct ccgaaaacga aaagatgttg atcgaaaagg tcagtgtcat tattcattct      360

gctgctaccg ttaagttcaa cgaaccattg ccaactgctt ggaagattaa cgttgaaggt      420

actagaatga tgttggcctt gtctagaaga atgaagagaa tcgaagtttt catccatatc      480

tctaccgctt acactaacac caacagagaa gttgttgacg aaatcttgta tccagctcca      540

gctgatattg atcaagttca ccaatatgtt aaggacggta tctctgaaga agaaactgaa      600

aaaatcttga acggtagacc aaacacttac actttcacta aggctttgac cgaacatttg      660

gttgctgaaa atcaagctta cgttccaacc attatcgtta gaccatcagt tgttgctgcc      720

attaaggatg aacctattaa gggttggttg ggtaattggt atggtgctac aggtttgact      780

gtttttactg ctaagggttt gaacagagtt atctacggtc actcttctaa catcgttgat      840

ttgatcccag ttgattacgt tgccaacttg gttattgctg ctggtgctaa atcttctaag      900

tctactgaat tgaaggtcta caactgctgt tcttctgctt gtaacccaat tactatcggt      960

aagttgatgt ccatgtttgc tgaagatgct atcaagcaaa agtcttacgc tatgccattg     1020

ccaggttggt acatttttac taagtacaag tggttggtct tgttgttgac cattttgttc     1080

caagttattc cagcctacat taccgacttg tacagacatt tgattggtaa gaacccaaga     1140

tatatcaagt tgcaatcctt ggtcaatcaa accagatcct ccattgattt cttcaccaac     1200

cattcttggg ttatgaaggc tgatagagtc agagaattat tcgcttcttt gtctccagca     1260

gataagtact tgtttccatg tgatccagtc aacatcaatt ggagacaata tatccaagat     1320

tactgctggg gtgttagaca tttcttggaa aaaaagactt aa                        1362


<210>  12
<211>  453
<212>  PRT
<213>  Heliothis subflexa

<400>  12

Met Val Val Leu Thr Ser Lys Glu Thr Lys Pro Ser Val Ala Glu Phe 
1               5                   10                  15      


Tyr Ala Gly Lys Ser Val Phe Ile Thr Gly Gly Thr Gly Phe Leu Gly 
            20                  25                  30          


Lys Val Phe Ile Glu Lys Leu Leu Tyr Ser Cys Pro Asp Ile Gly Asn 
        35                  40                  45              


Ile Tyr Met Leu Ile Arg Glu Lys Lys Gly Leu Ser Val Ser Glu Arg 
    50                  55                  60                  


Ile Lys His Phe Leu Asp Asp Pro Leu Phe Thr Arg Leu Lys Glu Lys 
65                  70                  75                  80  


Arg Pro Ala Asp Leu Glu Lys Ile Val Leu Ile Pro Gly Asp Ile Thr 
                85                  90                  95      


Ala Pro Asp Leu Gly Ile Thr Ser Glu Asn Glu Lys Met Leu Ile Glu 
            100                 105                 110         


Lys Val Ser Val Ile Ile His Ser Ala Ala Thr Val Lys Phe Asn Glu 
        115                 120                 125             


Pro Leu Pro Thr Ala Trp Lys Ile Asn Val Glu Gly Thr Arg Met Met 
    130                 135                 140                 


Leu Ala Leu Ser Arg Arg Met Lys Arg Ile Glu Val Phe Ile His Ile 
145                 150                 155                 160 


Ser Thr Ala Tyr Thr Asn Thr Asn Arg Glu Val Val Asp Glu Ile Leu 
                165                 170                 175     


Tyr Pro Ala Pro Ala Asp Ile Asp Gln Val His Gln Tyr Val Lys Asp 
            180                 185                 190         


Gly Ile Ser Glu Glu Glu Thr Glu Lys Ile Leu Asn Gly Arg Pro Asn 
        195                 200                 205             


Thr Tyr Thr Phe Thr Lys Ala Leu Thr Glu His Leu Val Ala Glu Asn 
    210                 215                 220                 


Gln Ala Tyr Val Pro Thr Ile Ile Val Arg Pro Ser Val Val Ala Ala 
225                 230                 235                 240 


Ile Lys Asp Glu Pro Ile Lys Gly Trp Leu Gly Asn Trp Tyr Gly Ala 
                245                 250                 255     


Thr Gly Leu Thr Val Phe Thr Ala Lys Gly Leu Asn Arg Val Ile Tyr 
            260                 265                 270         


Gly His Ser Ser Asn Ile Val Asp Leu Ile Pro Val Asp Tyr Val Ala 
        275                 280                 285             


Asn Leu Val Ile Ala Ala Gly Ala Lys Ser Ser Lys Ser Thr Glu Leu 
    290                 295                 300                 


Lys Val Tyr Asn Cys Cys Ser Ser Ala Cys Asn Pro Ile Thr Ile Gly 
305                 310                 315                 320 


Lys Leu Met Ser Met Phe Ala Glu Asp Ala Ile Lys Gln Lys Ser Tyr 
                325                 330                 335     


Ala Met Pro Leu Pro Gly Trp Tyr Ile Phe Thr Lys Tyr Lys Trp Leu 
            340                 345                 350         


Val Leu Leu Leu Thr Ile Leu Phe Gln Val Ile Pro Ala Tyr Ile Thr 
        355                 360                 365             


Asp Leu Tyr Arg His Leu Ile Gly Lys Asn Pro Arg Tyr Ile Lys Leu 
    370                 375                 380                 


Gln Ser Leu Val Asn Gln Thr Arg Ser Ser Ile Asp Phe Phe Thr Asn 
385                 390                 395                 400 


His Ser Trp Val Met Lys Ala Asp Arg Val Arg Glu Leu Phe Ala Ser 
                405                 410                 415     


Leu Ser Pro Ala Asp Lys Tyr Leu Phe Pro Cys Asp Pro Val Asn Ile 
            420                 425                 430         


Asn Trp Arg Gln Tyr Ile Gln Asp Tyr Cys Trp Gly Val Arg His Phe 
        435                 440                 445             


Leu Glu Lys Lys Thr 
    450             


<210>  13
<211>  1368
<212>  DNA
<213>  Artificial sequence

<220>
<223>  codon-optimized nucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(1368)
<223>  S. cerevisiae-codon-optimized nucleotide sequence of Helicoverpa 
       armigera fatty acyl reductase; mRNA-coding sequence

<400>  13
atggttgtct tgacctccaa agaaactaag ccatctgttg ctgaatttta cgctggtaag       60

tctgttttca ttactggtgg tactggtttc ttgggtaagg ttttcattga aaagttgttg      120

tactcctgcc cagatatcgg taatatctac atgttgatca gagaaaagaa gggtttgtcc      180

gtttccgaaa gaatcaagca ctttttggat gatcctttgt tcaccagatt gaaagaaaaa      240

agaccagccg acttggaaaa gatcgttttg attccaggtg atattactgc tccagatttg      300

ggtattacct ccgaaaacga aaagatgttg atcgaaaagg tcagtgtcat tattcattct      360

gctgctaccg ttaagttcaa cgaaccattg ccaactgctt ggaagattaa cgttgaaggt      420

actagaatga tgttggcctt gtctagaaga atgaagagaa tcgaagtttt catccatatc      480

tctaccgctt acactaacac caacagagaa gttgttgacg aaatcttgta tccagctcca      540

gctgatattg atcaagttca cagatatgtt aaggacggta tctctgaaga agaaactgaa      600

aaaatcttga acggtagacc aaacacttac actttcacta aggctttgac cgaacatttg      660

gttgctgaaa atcaagctta cgttccaacc attatcgtta gaccatcagt tgttgctgcc      720

attaaggatg aacctattaa gggttggttg ggtaattggt atggtgctac aggtttgact      780

gtttttactg ctaagggttt gaacagagtt atctacggtc actcttctaa catcgttgat      840

ttgatcccag ttgattacgt tgccaacttg gttattgctg ctggtgctaa atcttctaag      900

tctactgaat tgaaggtcta caactgctgt tcttctgctt gtaacccaat tactatcggt      960

aagttgatgt ccatgtttgc tgaagatgct atcaagcaaa agtcttacgc tatgccattg     1020

ccaggttggt acatttttac taagtacaag tggttggtct tgttgttgac cattttgttc     1080

caagttattc cagcctacat taccgacttg tacagacatt tgattggtaa gaacccaaga     1140

tatatcaagt tgcaatcctt ggtcaatcaa accagatcct ccattgattt cttcacctct     1200

cattcttggg ttatgaaggc tgatagagtc agagaattat tcgcttcttt gtctccagca     1260

gataagtact tgtttccatg tgatccaacc gatattaact ggacccatta cattcaagat     1320

tactgctggg gtgttagaca tttcttggaa aaaaaaagct acgaataa                  1368


<210>  14
<211>  455
<212>  PRT
<213>  Helicoverpa armigera

<400>  14

Met Val Val Leu Thr Ser Lys Glu Thr Lys Pro Ser Val Ala Glu Phe 
1               5                   10                  15      


Tyr Ala Gly Lys Ser Val Phe Ile Thr Gly Gly Thr Gly Phe Leu Gly 
            20                  25                  30          


Lys Val Phe Ile Glu Lys Leu Leu Tyr Ser Cys Pro Asp Ile Gly Asn 
        35                  40                  45              


Ile Tyr Met Leu Ile Arg Glu Lys Lys Gly Leu Ser Val Ser Glu Arg 
    50                  55                  60                  


Ile Lys His Phe Leu Asp Asp Pro Leu Phe Thr Arg Leu Lys Glu Lys 
65                  70                  75                  80  


Arg Pro Ala Asp Leu Glu Lys Ile Val Leu Ile Pro Gly Asp Ile Thr 
                85                  90                  95      


Ala Pro Asp Leu Gly Ile Thr Ser Glu Asn Glu Lys Met Leu Ile Glu 
            100                 105                 110         


Lys Val Ser Val Ile Ile His Ser Ala Ala Thr Val Lys Phe Asn Glu 
        115                 120                 125             


Pro Leu Pro Thr Ala Trp Lys Ile Asn Val Glu Gly Thr Arg Met Met 
    130                 135                 140                 


Leu Ala Leu Ser Arg Arg Met Lys Arg Ile Glu Val Phe Ile His Ile 
145                 150                 155                 160 


Ser Thr Ala Tyr Thr Asn Thr Asn Arg Glu Val Val Asp Glu Ile Leu 
                165                 170                 175     


Tyr Pro Ala Pro Ala Asp Ile Asp Gln Val His Arg Tyr Val Lys Asp 
            180                 185                 190         


Gly Ile Ser Glu Glu Glu Thr Glu Lys Ile Leu Asn Gly Arg Pro Asn 
        195                 200                 205             


Thr Tyr Thr Phe Thr Lys Ala Leu Thr Glu His Leu Val Ala Glu Asn 
    210                 215                 220                 


Gln Ala Tyr Val Pro Thr Ile Ile Val Arg Pro Ser Val Val Ala Ala 
225                 230                 235                 240 


Ile Lys Asp Glu Pro Ile Lys Gly Trp Leu Gly Asn Trp Tyr Gly Ala 
                245                 250                 255     


Thr Gly Leu Thr Val Phe Thr Ala Lys Gly Leu Asn Arg Val Ile Tyr 
            260                 265                 270         


Gly His Ser Ser Asn Ile Val Asp Leu Ile Pro Val Asp Tyr Val Ala 
        275                 280                 285             


Asn Leu Val Ile Ala Ala Gly Ala Lys Ser Ser Lys Ser Thr Glu Leu 
    290                 295                 300                 


Lys Val Tyr Asn Cys Cys Ser Ser Ala Cys Asn Pro Ile Thr Ile Gly 
305                 310                 315                 320 


Lys Leu Met Ser Met Phe Ala Glu Asp Ala Ile Lys Gln Lys Ser Tyr 
                325                 330                 335     


Ala Met Pro Leu Pro Gly Trp Tyr Ile Phe Thr Lys Tyr Lys Trp Leu 
            340                 345                 350         


Val Leu Leu Leu Thr Ile Leu Phe Gln Val Ile Pro Ala Tyr Ile Thr 
        355                 360                 365             


Asp Leu Tyr Arg His Leu Ile Gly Lys Asn Pro Arg Tyr Ile Lys Leu 
    370                 375                 380                 


Gln Ser Leu Val Asn Gln Thr Arg Ser Ser Ile Asp Phe Phe Thr Ser 
385                 390                 395                 400 


His Ser Trp Val Met Lys Ala Asp Arg Val Arg Glu Leu Phe Ala Ser 
                405                 410                 415     


Leu Ser Pro Ala Asp Lys Tyr Leu Phe Pro Cys Asp Pro Thr Asp Ile 
            420                 425                 430         


Asn Trp Thr His Tyr Ile Gln Asp Tyr Cys Trp Gly Val Arg His Phe 
        435                 440                 445             


Leu Glu Lys Lys Ser Tyr Glu 
    450                 455 


<210>  15
<211>  1371
<212>  DNA
<213>  Artificial sequence

<220>
<223>  codon-optimized nucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(1371)
<223>  S. cerevisiae-codon-optimized nucleotide sequence of H. assulta 
       fatty acyl reductase; mRNA-coding sequence.

<400>  15
atggttgtct tgacctccaa agaaactaag ccatctgttg ctgaatttta cgctggtaag       60

tctgttttca ttactggtgg tactggtttc ttgggtaaga tcttcattga aaagttgttg      120

tactcctgcc cagatatcgg taatatctac atgttgatca gagaaaagaa gggtttgtcc      180

gtttccgaaa gaatcaagca atttttggat gaccctttgt tcaccagatt gaaagaaaaa      240

agaccagccg acttggaaaa gatcgttttg attccaggtg atattactgc tccagatttg      300

ggtattacct ccgaaaacga aaagatgttg atcgaaaagg tcagtgtcat tattcattct      360

gctgctaccg ttaagttcaa cgaaccattg ccaactgctt ggaagattaa cgttgaaggt      420

actagaatga tgttggcctt gtctagaaga atgaagagaa tcgaagtttt catccatatc      480

tctaccgctt acactaacac caacagagaa gttgttgacg aaatcttgta tccagctcca      540

gctgatattg atcaagttca ccaatatgtt aaggacggta tctctgaaga agaaactgaa      600

aaaatcttga acggtagacc aaacacttac actttcacta aggctttgac cgaacatttg      660

gttgctgaaa atcaagctta cgttccaacc attatcgtta gaccatcagt tgttgctgcc      720

attaaggatg aacctattaa gggttggttg ggtaattggt atggtgctac aggtttgact      780

gtttttactg ctaagggttt gaacagagtt atctacggtc attcctctta catcgttgat      840

ttgatcccag ttgattacgt tgccaacttg gttattgctg ctggtgctaa atcttctaag      900

tctactgaat tgaaggtcta caactgctgt tcttctgctt gtaacccaat tactatcggt      960

aagttgatgt ccatgtttgc tgaagatgct atcaagcaaa agtcttacgc tatgccattg     1020

ccaggttggt atgtttttac aaagtacaag tggttggtct tgttgttgac cattttgttc     1080

caagttattc cagcctacat taccgacttg tacagacatt tgattggtaa gaacccaaga     1140

tatatcaagt tgcaatcctt ggtcaatcaa accagatcct ccattgattt cttcacctct     1200

cattcttggg ttatgaaggc tgatagagtc agagaattat tcgcttcttt gtctccagca     1260

gataagtact tgtttccatg tgatccaacc gatattaact ggacccatta cattcaagat     1320

tactgctggg gtgttagaca cttcttggaa aaaaagacta ccaacaagta a              1371


<210>  16
<211>  456
<212>  PRT
<213>  Helicoverpa assulta

<400>  16

Met Val Val Leu Thr Ser Lys Glu Thr Lys Pro Ser Val Ala Glu Phe 
1               5                   10                  15      


Tyr Ala Gly Lys Ser Val Phe Ile Thr Gly Gly Thr Gly Phe Leu Gly 
            20                  25                  30          


Lys Ile Phe Ile Glu Lys Leu Leu Tyr Ser Cys Pro Asp Ile Gly Asn 
        35                  40                  45              


Ile Tyr Met Leu Ile Arg Glu Lys Lys Gly Leu Ser Val Ser Glu Arg 
    50                  55                  60                  


Ile Lys Gln Phe Leu Asp Asp Pro Leu Phe Thr Arg Leu Lys Glu Lys 
65                  70                  75                  80  


Arg Pro Ala Asp Leu Glu Lys Ile Val Leu Ile Pro Gly Asp Ile Thr 
                85                  90                  95      


Ala Pro Asp Leu Gly Ile Thr Ser Glu Asn Glu Lys Met Leu Ile Glu 
            100                 105                 110         


Lys Val Ser Val Ile Ile His Ser Ala Ala Thr Val Lys Phe Asn Glu 
        115                 120                 125             


Pro Leu Pro Thr Ala Trp Lys Ile Asn Val Glu Gly Thr Arg Met Met 
    130                 135                 140                 


Leu Ala Leu Ser Arg Arg Met Lys Arg Ile Glu Val Phe Ile His Ile 
145                 150                 155                 160 


Ser Thr Ala Tyr Thr Asn Thr Asn Arg Glu Val Val Asp Glu Ile Leu 
                165                 170                 175     


Tyr Pro Ala Pro Ala Asp Ile Asp Gln Val His Gln Tyr Val Lys Asp 
            180                 185                 190         


Gly Ile Ser Glu Glu Glu Thr Glu Lys Ile Leu Asn Gly Arg Pro Asn 
        195                 200                 205             


Thr Tyr Thr Phe Thr Lys Ala Leu Thr Glu His Leu Val Ala Glu Asn 
    210                 215                 220                 


Gln Ala Tyr Val Pro Thr Ile Ile Val Arg Pro Ser Val Val Ala Ala 
225                 230                 235                 240 


Ile Lys Asp Glu Pro Ile Lys Gly Trp Leu Gly Asn Trp Tyr Gly Ala 
                245                 250                 255     


Thr Gly Leu Thr Val Phe Thr Ala Lys Gly Leu Asn Arg Val Ile Tyr 
            260                 265                 270         


Gly His Ser Ser Tyr Ile Val Asp Leu Ile Pro Val Asp Tyr Val Ala 
        275                 280                 285             


Asn Leu Val Ile Ala Ala Gly Ala Lys Ser Ser Lys Ser Thr Glu Leu 
    290                 295                 300                 


Lys Val Tyr Asn Cys Cys Ser Ser Ala Cys Asn Pro Ile Thr Ile Gly 
305                 310                 315                 320 


Lys Leu Met Ser Met Phe Ala Glu Asp Ala Ile Lys Gln Lys Ser Tyr 
                325                 330                 335     


Ala Met Pro Leu Pro Gly Trp Tyr Val Phe Thr Lys Tyr Lys Trp Leu 
            340                 345                 350         


Val Leu Leu Leu Thr Ile Leu Phe Gln Val Ile Pro Ala Tyr Ile Thr 
        355                 360                 365             


Asp Leu Tyr Arg His Leu Ile Gly Lys Asn Pro Arg Tyr Ile Lys Leu 
    370                 375                 380                 


Gln Ser Leu Val Asn Gln Thr Arg Ser Ser Ile Asp Phe Phe Thr Ser 
385                 390                 395                 400 


His Ser Trp Val Met Lys Ala Asp Arg Val Arg Glu Leu Phe Ala Ser 
                405                 410                 415     


Leu Ser Pro Ala Asp Lys Tyr Leu Phe Pro Cys Asp Pro Thr Asp Ile 
            420                 425                 430         


Asn Trp Thr His Tyr Ile Gln Asp Tyr Cys Trp Gly Val Arg His Phe 
        435                 440                 445             


Leu Glu Lys Lys Thr Thr Asn Lys 
    450                 455     


<210>  17
<211>  1086
<212>  DNA
<213>  Artificial sequence

<220>
<223>  codon-optimized nucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(1086)
<223>  S. cerevisiae codon-optimized nucleotide sequence of delat9 
       desaturase from Drosophila melanogaster Dmd9

<400>  17
atggctccat actctagaat ctaccaccaa gataagtcct ctagagaaac tggtgttttg       60

ttcgaagatg atgctcaaac cgttgattct gatttgacta ccgatagatt ccaattgaag      120

agagccgaaa aaagaagatt gccattggtt tggagaaaca tcatcttgtt cgctttggtt      180

catttggctg ccttgtatgg tttacattcc attttcacta gagctaagtt ggctactact      240

ttgtttgctg ctggtttgta cattatcggt atgttgggtg ttactgctgg tgctcataga      300

ttgtgggctc atagaactta caaagctaaa tggcctttga gattgttgtt ggtcatcttc      360

aacaccattg ctttccaaga tgctgtttat cattgggcca gagatcatag agttcatcac      420

aaatactctg aaaccgatgc tgatccacat aatgctacta gaggtttctt cttctctcat      480

gttggttggt tgttgtgcaa gaaacaccca gatatcaaag aaaagggtag aggtttggat      540

ttgtccgatt tgagagctga tccaatcttg atgtttcaaa gaaagcacta ctacatcttg      600

atgccattgg cttgttttgt tttgccaacc gttattccaa tggtctactg gaacgaaact      660

ttggcttctt cttggtttgt tgctactatg ttcagatggt gcttccaatt gaatatgacc      720

tggttggtta attccgctgc tcataagttt ggtaatagac catacgataa gaccatgaac      780

ccaactcaaa atgctttcgt ttctgctttc acttttggtg aaggttggca taattaccat      840

catgcttttc catgggatta caagactgct gaatggggtt gttactcttt gaacattact      900

accgccttca ttgatttgtt cgctaaaatt ggttgggcct acgatttgaa aactgttgct      960

ccagatgtta tccaaagaag agttttgaga actggtgatg gttctcatga attgtggggt     1020

tggggtgata aggatttgac cgctgaagat gctagaaacg ttttgttggt tgacaagtcc     1080

agataa                                                                1086


<210>  18
<211>  361
<212>  PRT
<213>  Drosophila melanogaster

<400>  18

Met Ala Pro Tyr Ser Arg Ile Tyr His Gln Asp Lys Ser Ser Arg Glu 
1               5                   10                  15      


Thr Gly Val Leu Phe Glu Asp Asp Ala Gln Thr Val Asp Ser Asp Leu 
            20                  25                  30          


Thr Thr Asp Arg Phe Gln Leu Lys Arg Ala Glu Lys Arg Arg Leu Pro 
        35                  40                  45              


Leu Val Trp Arg Asn Ile Ile Leu Phe Ala Leu Val His Leu Ala Ala 
    50                  55                  60                  


Leu Tyr Gly Leu His Ser Ile Phe Thr Arg Ala Lys Leu Ala Thr Thr 
65                  70                  75                  80  


Leu Phe Ala Ala Gly Leu Tyr Ile Ile Gly Met Leu Gly Val Thr Ala 
                85                  90                  95      


Gly Ala His Arg Leu Trp Ala His Arg Thr Tyr Lys Ala Lys Trp Pro 
            100                 105                 110         


Leu Arg Leu Leu Leu Val Ile Phe Asn Thr Ile Ala Phe Gln Asp Ala 
        115                 120                 125             


Val Tyr His Trp Ala Arg Asp His Arg Val His His Lys Tyr Ser Glu 
    130                 135                 140                 


Thr Asp Ala Asp Pro His Asn Ala Thr Arg Gly Phe Phe Phe Ser His 
145                 150                 155                 160 


Val Gly Trp Leu Leu Cys Lys Lys His Pro Asp Ile Lys Glu Lys Gly 
                165                 170                 175     


Arg Gly Leu Asp Leu Ser Asp Leu Arg Ala Asp Pro Ile Leu Met Phe 
            180                 185                 190         


Gln Arg Lys His Tyr Tyr Ile Leu Met Pro Leu Ala Cys Phe Val Leu 
        195                 200                 205             


Pro Thr Val Ile Pro Met Val Tyr Trp Asn Glu Thr Leu Ala Ser Ser 
    210                 215                 220                 


Trp Phe Val Ala Thr Met Phe Arg Trp Cys Phe Gln Leu Asn Met Thr 
225                 230                 235                 240 


Trp Leu Val Asn Ser Ala Ala His Lys Phe Gly Asn Arg Pro Tyr Asp 
                245                 250                 255     


Lys Thr Met Asn Pro Thr Gln Asn Ala Phe Val Ser Ala Phe Thr Phe 
            260                 265                 270         


Gly Glu Gly Trp His Asn Tyr His His Ala Phe Pro Trp Asp Tyr Lys 
        275                 280                 285             


Thr Ala Glu Trp Gly Cys Tyr Ser Leu Asn Ile Thr Thr Ala Phe Ile 
    290                 295                 300                 


Asp Leu Phe Ala Lys Ile Gly Trp Ala Tyr Asp Leu Lys Thr Val Ala 
305                 310                 315                 320 


Pro Asp Val Ile Gln Arg Arg Val Leu Arg Thr Gly Asp Gly Ser His 
                325                 330                 335     


Glu Leu Trp Gly Trp Gly Asp Lys Asp Leu Thr Ala Glu Asp Ala Arg 
            340                 345                 350         


Asn Val Leu Leu Val Asp Lys Ser Arg 
        355                 360     


<210>  19
<211>  335
<212>  PRT
<213>  Choristoneura rosaceana

<400>  19

Met Ala Pro Asn Val Glu Asp Met Glu Ser Asp Leu Pro Glu Ser Glu 
1               5                   10                  15      


Glu Lys Leu Glu Lys Leu Val Ala Pro Gln Ala Ala Pro Arg Lys Tyr 
            20                  25                  30          


Gln Ile Ile Tyr Thr Asn Leu Leu Thr Phe Gly Tyr Trp His Ile Ala 
        35                  40                  45              


Gly Leu Tyr Gly Leu Tyr Leu Cys Phe Thr Ser Ala Lys Trp Gln Thr 
    50                  55                  60                  


Ile Ile Leu Ala Leu Ile Leu Asn Glu Met Ala Ile Leu Gly Ile Thr 
65                  70                  75                  80  


Ala Gly Ala His Arg Leu Trp Ala His Arg Ser Tyr Lys Ala Thr Val 
                85                  90                  95      


Pro Leu Gln Ile Ile Leu Ile Ile Phe Asn Ser Leu Ser Phe Gln Asn 
            100                 105                 110         


Ser Ala Ile His Trp Ile Arg Asp His Arg Met His His Lys Tyr Ser 
        115                 120                 125             


Asp Thr Asp Gly Asp Pro His Asn Ala Ser Arg Gly Phe Phe Tyr Ser 
    130                 135                 140                 


His Val Gly Trp Leu Leu Val Lys Lys His Pro Glu Val Lys Lys Arg 
145                 150                 155                 160 


Ala Lys Thr Ile Asp Met Ser Asp Ile Tyr Ser Asn Pro Ile Leu Arg 
                165                 170                 175     


Phe Gln Lys Lys Tyr Ala Ile Pro Phe Ile Gly Met Ile Cys Phe Val 
            180                 185                 190         


Leu Pro Thr Ile Ile Pro Met Tyr Phe Trp Gly Glu Thr Leu Ser Asn 
        195                 200                 205             


Ala Trp His Ile Thr Met Leu Arg Tyr Val Phe Ser Leu Asn Ser Ile 
    210                 215                 220                 


Phe Leu Val Asn Ser Ala Ala His Leu Tyr Gly Tyr Arg Pro Tyr Asp 
225                 230                 235                 240 


Lys Asn Ile Leu Pro Ala Glu Asn Lys Met Thr Phe Ile Ala Cys Leu 
                245                 250                 255     


Gly Glu Asn Phe His Asn Tyr His His Val Phe Pro Trp Asp Tyr Arg 
            260                 265                 270         


Ala Ser Glu Leu Gly Asn Ile Gly Met Asn Trp Thr Ala Lys Phe Ile 
        275                 280                 285             


Asp Phe Phe Ala Trp Ile Gly Trp Ala Tyr Asp Leu Lys Thr Ala Ser 
    290                 295                 300                 


Asp Glu Asn Ile Lys Ser Arg Met Lys Arg Thr Gly Asp Gly Thr Asp 
305                 310                 315                 320 


Val Ser Gly Gln Lys Tyr Ser Cys Glu Ser Ser Glu Val Leu Gln 
                325                 330                 335 


<210>  20
<211>  334
<212>  PRT
<213>  Choristoneura parallela

<400>  20

Met Ala Pro Asn Val Glu Asp Met Glu Ser Asp Met Pro Glu Ser Glu 
1               5                   10                  15      


Lys Trp Glu Lys Leu Val Ala Pro Gln Ala Ala Pro Arg Lys Tyr Glu 
            20                  25                  30          


Ile Ile Tyr Thr Asn Leu Leu Thr Phe Gly Tyr Gly His Ile Ala Gly 
        35                  40                  45              


Leu Tyr Gly Leu Tyr Leu Cys Phe Thr Ser Ala Lys Trp Gln Thr Val 
    50                  55                  60                  


Ile Leu Ala Ile Ile Leu Asn Glu Met Ala Ile Leu Gly Ile Thr Ala 
65                  70                  75                  80  


Gly Ala His Arg Leu Trp Ser His Arg Ser Tyr Lys Ala Ala Val Pro 
                85                  90                  95      


Leu Gln Ile Ile Leu Met Ile Phe Asn Ser Leu Ala Phe Gln Asn Ser 
            100                 105                 110         


Ala Ile Asn Trp Val Arg Asp His Arg Met His His Lys Tyr Ser Asp 
        115                 120                 125             


Thr Asp Gly Asp Pro His Asn Ala Ser Arg Gly Phe Phe Tyr Ser His 
    130                 135                 140                 


Val Gly Trp Leu Leu Val Lys Lys His Pro Glu Val Lys Lys Arg Gly 
145                 150                 155                 160 


Lys Met Ile Asp Met Ser Asp Ile Tyr Ser Asn Pro Val Leu Arg Phe 
                165                 170                 175     


Gln Lys Lys Tyr Ala Ile Pro Phe Ile Gly Met Ile Cys Phe Val Leu 
            180                 185                 190         


Pro Thr Ile Ile Pro Met Tyr Phe Trp Gly Glu Thr Leu Ser Asn Ala 
        195                 200                 205             


Trp His Ile Thr Met Leu Arg Tyr Val Phe Ser Leu Asn Ser Ile Phe 
    210                 215                 220                 


Leu Val Asn Ser Ala Ala His Leu Tyr Gly Tyr Arg Pro Tyr Asp Lys 
225                 230                 235                 240 


Asn Ile Leu Pro Ala Glu Asn Lys Ile Ala Leu Ile Ala Cys Leu Gly 
                245                 250                 255     


Asp Ser Phe His Asn Tyr His His Val Phe Pro Trp Asp Tyr Arg Ala 
            260                 265                 270         


Ser Glu Leu Gly Asn Ile Gly Met Asn Trp Thr Ala Gln Phe Ile Asp 
        275                 280                 285             


Phe Phe Ala Trp Ile Gly Trp Ala Tyr Asp Leu Lys Thr Ala Ser Asp 
    290                 295                 300                 


Glu Asn Ile Asn Ser Arg Met Lys Arg Thr Gly Asp Gly Thr Asp Ile 
305                 310                 315                 320 


Ser Gly Gln Lys Tyr Ser Cys Glu Ser Ser Glu Val Leu Gln 
                325                 330                 


<210>  21
<211>  1830
<212>  DNA
<213>  Yarrowia lipolytica

<400>  21
atgtctgacg acaagcacac tttcgacttt atcattgtcg gtggaggaac cgccggcccc       60

actctcgccc ggcgactggc cgatgcctgg atctccggta agaagctcaa ggtgctcctg      120

ctcgagtccg gcccctcttc cgagggtgtt gatgatattc gatgccccgg taactgggtc      180

aacaccatcc actccgagta cgactggtcc tacgaggtcg acgagcctta cctgtctact      240

gatggcgagg agcgacgact ctgtggtatc ccccgaggcc attgtctggg tggatcctct      300

tgtctgaaca cctctttcgt catccgagga acccgaggtg atttcgaccg aatcgaagag      360

gagaccggcg ctaagggctg gggttgggat gatctgttcc cctacttccg aaagcacgag      420

tgttacgtgc cccagggatc tgcccacgag cccaagctca ttgacttcga cacctacgac      480

tacaagaagt tccacggtga ctctggtcct atcaaggtcc agccttacga ctacgcgccc      540

atctccaaga agttctctga gtctctggct tctttcggct acccttataa ccccgagatc      600

ttcgtcaacg gaggagcccc ccagggttgg ggtcacgttg ttcgttccac ctccaacggt      660

gttcgatcca ccggctacga cgctcttgtc cacgccccca agaacctcga cattgtgact      720

ggccacgctg tcaccaagat tctctttgag aagatcggtg gcaagcagac cgccgttggt      780

gtcgagacct acaaccgagc tgccgaggag gctggcccta cctacaaggc ccgatacgag      840

gtggttgtgt gctgcggctc ttatgcctct ccccagcttc tgatggtttc cggtgttgga      900

cccaagaagg agctcgagga ggttggtgtc aaggacatca ttttggactc tccttacgtt      960

ggaaagaacc tgcaggacca tcttatctgc ggtatctttg tcgaaattaa ggagcccgga     1020

tacacccgag accaccagtt cttcgacgac gagggactcg acaagtccac cgaggagtgg     1080

aagaccaagc gaaccggttt cttctccaat cctccccagg gcattttctc ttacggccga     1140

atcgacaacc tgctcaagga tgatcccgtc tggaaggagg cctgcgagaa gcagaaggct     1200

ctcaaccctc gacgagaccc catgggtaac gatccctctc agccccattt cgagatctgg     1260

aatgctgagc tctacatcga gctagagatg acccaggctc ccgacgaggg ccagtccgtc     1320

atgaccgtca tcggtgagat tcttcctcct cgatccaagg gttacgtcaa gctgctgtcc     1380

cccgacccta tggagaaccc cgagattgtc cacaactacc tgcaggaccc tgttgacgct     1440

cgagtcttcg ctgccatcat gaagcacgcc gccgacgttg ccaccaacgg tgctggcacc     1500

aaggacctcg tcaaggctcg atggcccccg gagtccaagc ccttcgagga aatgtccatc     1560

gaggaatggg agacttacgt ccgagacaag tctcacacct gtttccaccc ctgtggtact     1620

gtcaagcttg gtggtgctaa tgataaggag gccgttgttg acgagcgact ccgagtcaag     1680

ggtgtcgacg gcctgcgagt tgccgacgtc tctgtccttc cccgagtccc caacggacac     1740

acccaggctt ttgcctacgc tgttggtgag aaggctgccg acctcatcct tgccgacatt     1800

gctggaaagg atctccgacc tcgaatctaa                                      1830


<210>  22
<211>  609
<212>  PRT
<213>  Yarrowia lipolytica

<400>  22

Met Ser Asp Asp Lys His Thr Phe Asp Phe Ile Ile Val Gly Gly Gly 
1               5                   10                  15      


Thr Ala Gly Pro Thr Leu Ala Arg Arg Leu Ala Asp Ala Trp Ile Ser 
            20                  25                  30          


Gly Lys Lys Leu Lys Val Leu Leu Leu Glu Ser Gly Pro Ser Ser Glu 
        35                  40                  45              


Gly Val Asp Asp Ile Arg Cys Pro Gly Asn Trp Val Asn Thr Ile His 
    50                  55                  60                  


Ser Glu Tyr Asp Trp Ser Tyr Glu Val Asp Glu Pro Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Gly Glu Glu Arg Arg Leu Cys Gly Ile Pro Arg Gly His Cys Leu 
                85                  90                  95      


Gly Gly Ser Ser Cys Leu Asn Thr Ser Phe Val Ile Arg Gly Thr Arg 
            100                 105                 110         


Gly Asp Phe Asp Arg Ile Glu Glu Glu Thr Gly Ala Lys Gly Trp Gly 
        115                 120                 125             


Trp Asp Asp Leu Phe Pro Tyr Phe Arg Lys His Glu Cys Tyr Val Pro 
    130                 135                 140                 


Gln Gly Ser Ala His Glu Pro Lys Leu Ile Asp Phe Asp Thr Tyr Asp 
145                 150                 155                 160 


Tyr Lys Lys Phe His Gly Asp Ser Gly Pro Ile Lys Val Gln Pro Tyr 
                165                 170                 175     


Asp Tyr Ala Pro Ile Ser Lys Lys Phe Ser Glu Ser Leu Ala Ser Phe 
            180                 185                 190         


Gly Tyr Pro Tyr Asn Pro Glu Ile Phe Val Asn Gly Gly Ala Pro Gln 
        195                 200                 205             


Gly Trp Gly His Val Val Arg Ser Thr Ser Asn Gly Val Arg Ser Thr 
    210                 215                 220                 


Gly Tyr Asp Ala Leu Val His Ala Pro Lys Asn Leu Asp Ile Val Thr 
225                 230                 235                 240 


Gly His Ala Val Thr Lys Ile Leu Phe Glu Lys Ile Gly Gly Lys Gln 
                245                 250                 255     


Thr Ala Val Gly Val Glu Thr Tyr Asn Arg Ala Ala Glu Glu Ala Gly 
            260                 265                 270         


Pro Thr Tyr Lys Ala Arg Tyr Glu Val Val Val Cys Cys Gly Ser Tyr 
        275                 280                 285             


Ala Ser Pro Gln Leu Leu Met Val Ser Gly Val Gly Pro Lys Lys Glu 
    290                 295                 300                 


Leu Glu Glu Val Gly Val Lys Asp Ile Ile Leu Asp Ser Pro Tyr Val 
305                 310                 315                 320 


Gly Lys Asn Leu Gln Asp His Leu Ile Cys Gly Ile Phe Val Glu Ile 
                325                 330                 335     


Lys Glu Pro Gly Tyr Thr Arg Asp His Gln Phe Phe Asp Asp Glu Gly 
            340                 345                 350         


Leu Asp Lys Ser Thr Glu Glu Trp Lys Thr Lys Arg Thr Gly Phe Phe 
        355                 360                 365             


Ser Asn Pro Pro Gln Gly Ile Phe Ser Tyr Gly Arg Ile Asp Asn Leu 
    370                 375                 380                 


Leu Lys Asp Asp Pro Val Trp Lys Glu Ala Cys Glu Lys Gln Lys Ala 
385                 390                 395                 400 


Leu Asn Pro Arg Arg Asp Pro Met Gly Asn Asp Pro Ser Gln Pro His 
                405                 410                 415     


Phe Glu Ile Trp Asn Ala Glu Leu Tyr Ile Glu Leu Glu Met Thr Gln 
            420                 425                 430         


Ala Pro Asp Glu Gly Gln Ser Val Met Thr Val Ile Gly Glu Ile Leu 
        435                 440                 445             


Pro Pro Arg Ser Lys Gly Tyr Val Lys Leu Leu Ser Pro Asp Pro Met 
    450                 455                 460                 


Glu Asn Pro Glu Ile Val His Asn Tyr Leu Gln Asp Pro Val Asp Ala 
465                 470                 475                 480 


Arg Val Phe Ala Ala Ile Met Lys His Ala Ala Asp Val Ala Thr Asn 
                485                 490                 495     


Gly Ala Gly Thr Lys Asp Leu Val Lys Ala Arg Trp Pro Pro Glu Ser 
            500                 505                 510         


Lys Pro Phe Glu Glu Met Ser Ile Glu Glu Trp Glu Thr Tyr Val Arg 
        515                 520                 525             


Asp Lys Ser His Thr Cys Phe His Pro Cys Gly Thr Val Lys Leu Gly 
    530                 535                 540                 


Gly Ala Asn Asp Lys Glu Ala Val Val Asp Glu Arg Leu Arg Val Lys 
545                 550                 555                 560 


Gly Val Asp Gly Leu Arg Val Ala Asp Val Ser Val Leu Pro Arg Val 
                565                 570                 575     


Pro Asn Gly His Thr Gln Ala Phe Ala Tyr Ala Val Gly Glu Lys Ala 
            580                 585                 590         


Ala Asp Leu Ile Leu Ala Asp Ile Ala Gly Lys Asp Leu Arg Pro Arg 
        595                 600                 605             


Ile 
    


<210>  23
<211>  1437
<212>  DNA
<213>  Artificial sequence

<220>
<223>  codon-optimized nucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(1437)
<223>  Y.lipolytica codon-optimized nucleotide sequence of fatty acyl 
       reductase from Bicyclus anynana Ban-wFAR2

<400>  23
atgtaccgaa acgtgaacaa caactacaag ggccactctg tgtacaccaa cggcgctggc       60

tctcgagtga agtctctgct gtcctctgcc accgacaaca ccaacgagta ccagtctatc      120

gccgagtgct acaagggaca gtccgtgttc atcaccggcg gcaccggctt cgtcggcaag      180

gtgctgctcg agaagctgct gtactcttgc cccggcatcg acaaggtgta cctgctggtg      240

cgagagactc agggcgccac cgctcaccag cgaatgcaga agctcctgga agaacccgcc      300

ttctcgcgaa tcaaggaaga gaaccctcag gccttcgaga aggtgatccc catcgtgggc      360

gacatcaccc agcctcagct gggcatcatg gccgagaacg aggaactgct gattaaggaa      420

gtgtctttcg tctaccacgt ggccgccacc accaagttca acgagactct ggacattgcc      480

atgaacgtga acgtggccgg aaccggacga gtgctggacc tgtctaagcg aatggaaaac      540

atcaaggcct tcgtgtacgt gtctaccgcc tactctaaca ccgaccgaga ggtggtggaa      600

gaggtgctgt accccgctcc tgtgtctctg aacgaggtgc acaagctgct gaagatcggc      660

atcaccgacg ctcaggtgaa ggaactgatc aagggacgac ccaacaccta caccttcacc      720

aaggctctgg ctgagaacct ggtggccgac aaccacggac acgtgcccgc catcatcgtg      780

cgaccctcta tcgtgtcctc gtctaagaag gaacccatca ccggatggat cgactcttgg      840

tacggcgcca ccttcctggc caccgtgacc atgaagggct tcaaccgagt gttcgtgtcg      900

tcttacgagt acaacctgga cttcatcccc gtggactacg tgtccaacct gatcatcgtg      960

gccgctgctc gatgcaagtg ctctgacaag gtggacgtgt acaactcttc tacctccggc     1020

gaaaaccctc tgaagattgg cgccttcttc gacgacatca ttgcctactc ttgcaagcac     1080

aagttctacg acatccctct gcctatggcc tacctgactc gataccgatg ggtcatgttc     1140

ctgatcaccc tgctgctgca gaccctgcct gcctatatcg ccgacctgtt cctgctgatc     1200

gtgggcaaga agccccgata cgtcaagctg gcctctaaga tctctgccgc tcacgaggtc     1260

ctggactact tcccctctcg aacctggtct atgtctgccc gacagaccac cgctctgttc     1320

cagtctctgt ctccctcgga ccgagatcag tttccttgcg accccaccga catcgactgg     1380

aaggagtaca tcgtcaccta ctgccaggga atccgacagt tcctgtgcaa gtcttaa        1437


<210>  24
<211>  478
<212>  PRT
<213>  Bicyclus anynana

<400>  24

Met Tyr Arg Asn Val Asn Asn Asn Tyr Lys Gly His Ser Val Tyr Thr 
1               5                   10                  15      


Asn Gly Ala Gly Ser Arg Val Lys Ser Leu Leu Ser Ser Ala Thr Asp 
            20                  25                  30          


Asn Thr Asn Glu Tyr Gln Ser Ile Ala Glu Cys Tyr Lys Gly Gln Ser 
        35                  40                  45              


Val Phe Ile Thr Gly Gly Thr Gly Phe Val Gly Lys Val Leu Leu Glu 
    50                  55                  60                  


Lys Leu Leu Tyr Ser Cys Pro Gly Ile Asp Lys Val Tyr Leu Leu Val 
65                  70                  75                  80  


Arg Glu Thr Gln Gly Ala Thr Ala His Gln Arg Met Gln Lys Leu Leu 
                85                  90                  95      


Glu Glu Pro Ala Phe Ser Arg Ile Lys Glu Glu Asn Pro Gln Ala Phe 
            100                 105                 110         


Glu Lys Val Ile Pro Ile Val Gly Asp Ile Thr Gln Pro Gln Leu Gly 
        115                 120                 125             


Ile Met Ala Glu Asn Glu Glu Leu Leu Ile Lys Glu Val Ser Phe Val 
    130                 135                 140                 


Tyr His Val Ala Ala Thr Thr Lys Phe Asn Glu Thr Leu Asp Ile Ala 
145                 150                 155                 160 


Met Asn Val Asn Val Ala Gly Thr Gly Arg Val Leu Asp Leu Ser Lys 
                165                 170                 175     


Arg Met Glu Asn Ile Lys Ala Phe Val Tyr Val Ser Thr Ala Tyr Ser 
            180                 185                 190         


Asn Thr Asp Arg Glu Val Val Glu Glu Val Leu Tyr Pro Ala Pro Val 
        195                 200                 205             


Ser Leu Asn Glu Val His Lys Leu Leu Lys Ile Gly Ile Thr Asp Ala 
    210                 215                 220                 


Gln Val Lys Glu Leu Ile Lys Gly Arg Pro Asn Thr Tyr Thr Phe Thr 
225                 230                 235                 240 


Lys Ala Leu Ala Glu Asn Leu Val Ala Asp Asn His Gly His Val Pro 
                245                 250                 255     


Ala Ile Ile Val Arg Pro Ser Ile Val Ser Ser Ser Lys Lys Glu Pro 
            260                 265                 270         


Ile Thr Gly Trp Ile Asp Ser Trp Tyr Gly Ala Thr Phe Leu Ala Thr 
        275                 280                 285             


Val Thr Met Lys Gly Phe Asn Arg Val Phe Val Ser Ser Tyr Glu Tyr 
    290                 295                 300                 


Asn Leu Asp Phe Ile Pro Val Asp Tyr Val Ser Asn Leu Ile Ile Val 
305                 310                 315                 320 


Ala Ala Ala Arg Cys Lys Cys Ser Asp Lys Val Asp Val Tyr Asn Ser 
                325                 330                 335     


Ser Thr Ser Gly Glu Asn Pro Leu Lys Ile Gly Ala Phe Phe Asp Asp 
            340                 345                 350         


Ile Ile Ala Tyr Ser Cys Lys His Lys Phe Tyr Asp Ile Pro Leu Pro 
        355                 360                 365             


Met Ala Tyr Leu Thr Arg Tyr Arg Trp Val Met Phe Leu Ile Thr Leu 
    370                 375                 380                 


Leu Leu Gln Thr Leu Pro Ala Tyr Ile Ala Asp Leu Phe Leu Leu Ile 
385                 390                 395                 400 


Val Gly Lys Lys Pro Arg Tyr Val Lys Leu Ala Ser Lys Ile Ser Ala 
                405                 410                 415     


Ala His Glu Val Leu Asp Tyr Phe Pro Ser Arg Thr Trp Ser Met Ser 
            420                 425                 430         


Ala Arg Gln Thr Thr Ala Leu Phe Gln Ser Leu Ser Pro Ser Asp Arg 
        435                 440                 445             


Asp Gln Phe Pro Cys Asp Pro Thr Asp Ile Asp Trp Lys Glu Tyr Ile 
    450                 455                 460                 


Val Thr Tyr Cys Gln Gly Ile Arg Gln Phe Leu Cys Lys Ser 
465                 470                 475             


<210>  25
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(22)
<223>  SEQ ID NO: 25 PR-141n = uracil

<400>  25
agaacagcng aagcttcgta cg                                                22


<210>  26
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  PR-142n = uracil

<400>  26
aggccacnag tggatctgat atcac                                             25


<210>  27
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(29)
<223>  PR-10851 (Atrd11 expression cassette _fw) n = uracil

<400>  27
agtgcaggng acgcagtagg atgtcctgc                                         29


<210>  28
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(23)
<223>  PR-10853 (Hs_Far expression cassette _fw)n = uracil

<400>  28
acctgcacna gagaccgggt tgg                                               23


<210>  29
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  PR-10655 (EpiVecYL _fw)n = uracil

<400>  29
acccattgcn gtagatatgt cttgtgtgta agg                                    33


<210>  30
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(32)
<223>  PR-10656 (EpiVecYL _rev)n = uracil

<400>  30
atcatgtaan tagttatgtc acgcttacat tc                                     32


<210>  31
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(22)
<223>  PR-10702 (delta-pex10YL_up _fw)

<400>  31
cattgtaact agtcctggag gg                                                22


<210>  32
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  PR-10703 (delta-pex10YL_up _rev)n = uracil

<400>  32
acgaagttan tttgagccga ggcagatttg                                        30


<210>  33
<211>  31
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(31)
<223>  PR-10704 (dekta-pex10YL_down _fw)n = uracil

<400>  33
acgaagttat ntgacgaggt ctggatggaa g                                      31


<210>  34
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  PR-10705 (delta-pex10YL_down _rev)

<400>  34
cattgctaag aatccaaact ggag                                              24


<210>  35
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  PR-10767 (NatMxSynYL-start _rev_new)

<400>  35
tcatggacat ggcatagac                                                    19


<210>  36
<211>  51
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(51)
<223>  PR-11047 (NatMxSynYL-end _rev_new)n = uracil

<400>  36
aataacttcg natagcatac attatacgaa gttatcgagc gtcccaaaac c                51


<210>  37
<211>  31
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(31)
<223>  PR-11110 (E.coli backboneUSER _fw)

<400>  37
atcgcgtgca ttcgcggccg catttaaatc c                                      31


<210>  38
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(36)
<223>  PR-11111 (E.coli backboneUSER _rev)

<400>  38
tcgcacgcat tcgcggccgc aaatttaaat aaaatg                                 36


<210>  39
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  PR-11138 (Hphsyn _fw)n = uracil

<400>  39
agcaatgggn aaaaagcctg aactcaccgc                                        30


<210>  40
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  PR-11139 (Hphsyn _rev)n = uracil

<400>  40
attacatgan tattcctttg ccctcggacg                                        30


<210>  41
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  PR-11694 (GPAT_up_USER _fw)n = uracil

<400>  41
cgtgcgangc atctaggagc tccattcagc                                        30


<210>  42
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  PR-11695 (GPAT_down _USER_rev)n = uracil

<400>  42
cacgcgangg acgagcagac cacg                                              24


<210>  43
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  PR-13494 (Nat-Tcyc-loxP_fw)n = uracil

<400>  43
agggtacnac tttggatgat actgc                                             25


<210>  44
<211>  54
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(54)
<223>  PR-13549 (loxP-PrTefIntron _fw)n= uracil

<400>  44
ataacttcgn ataatgtatg ctatacgaag ttatagagac cgggttggcg gcgc             54


<210>  45
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  PR-14269 (UraYL_fw)

<400>  45
atgccctcct acgaggcccg                                                   20


<210>  46
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(22)
<223>  PR-14270 (UraYL_rev)

<400>  46
ctagcagttg atcttctggt ag                                                22


<210>  47
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  PR-15426 (?hfd1_up _fw)n = uracil

<400>  47
cgtgcganat aagaaaaaaa acag                                              24


<210>  48
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(26)
<223>  PR-15427 (delta-hfd1_up _rev)n = uracil

<400>  48
agctgttcna ctaaccctac ttcctc                                            26


<210>  49
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  PR-15428 (delta-hfd1_down _fw)n = uracil

<400>  49
agtggccntt ttattggtgg tgtg                                              24


<210>  50
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  PR-15429 (delta-hfd1_down _rev)n = uracil

<400>  50
cacgcgangc atagtgcttt tcatattc                                          28


<210>  51
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(32)
<223>  PR-15438 (delta-hfd4_up _fw)n = uracil

<400>  51
cgtgcganag tatcgctact gtactaaaat tg                                     32


<210>  52
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(29)
<223>  PR-15439 (delta-hfd4_up _rev)n = uracil

<400>  52
agctgttcna gcggacaagt gtcaatgtt                                         29


<210>  53
<211>  31
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(31)
<223>  PR-15440 (delta-hfd4_down _fw)n = uracil

<400>  53
agtggccnat gtattttatc agtagtatct c                                      31


<210>  54
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  PR-15441 (delta-hfd4_down _rev)n = uracil

<400>  54
cacgcganat tggataatac atttccta                                          28


<210>  55
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  PR-16463 (delta-fao1YL_up _fw)n = uracil

<400>  55
cgtgcgantg ggggaggatt gcgatggg                                          28


<210>  56
<211>  31
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(31)
<223>  PR-16464 (delta-fao1YL_up _rev)n = uracil

<400>  56
agctgttcnt gtcaagtaat caagctaatg c                                      31


<210>  57
<211>  31
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(31)
<223>  PR-16465 (delta-fao1YL_down _fw)n = uracil

<400>  57
agtggccngc aagagacgag tttagaaata g                                      31


<210>  58
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(29)
<223>  PR-16466 (delta-fao1YL_down _rev)n = uracil

<400>  58
cacgcgangt gttagttcct tgtagtgtg                                         29


<210>  59
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(29)
<223>  PR-16696 (GPAT_up _rev)n = uracil

<400>  59
agctgttcnt accgcacttc cggaacatc                                         29


<210>  60
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  PR-16698 (GPAT_100bpPr_down _fw)n = uracil

<400>  60
agtggccncc gatacttgtt tgtgtgac                                          28


<210>  61
<211>  1578
<212>  DNA
<213>  Artificial sequence

<220>
<223>  codon-optimized nucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(1578)
<223>  Y.lipolytica codon-optimized nucleotide sequence of alcohol 
       acetyltransferase from S. cerevisiae ATF1

<400>  61
atgaacgaga tcgacgagaa gaaccaggct cctgtgcagc aagagtgcct gaaggaaatg       60

atccagaacg gacacgcccg acgaatgggc tctgtcgagg acctgtacgt ggccctgaac      120

cgacagaacc tgtaccgaaa cttctgcacc tacggcgagc tgtctgacta ctgcacccga      180

gatcagctga ccctggctct gcgagagatc tgcctgaaga accctactct gctgcatatc      240

gtgctgccca ctcgatggcc caaccacgag aactactacc gatcttctga gtactactct      300

cgaccccatc ctgtgcacga ctacatctcc gtgctgcaag agctgaagct gtctggcgtg      360

gtgctgaacg agcagcccga gtactctgcc gtgatgaagc agatcctgga agagttcaag      420

aactctaagg gctcttacac cgccaagatc ttcaagctga ctactaccct gaccattcct      480

tacttcggcc ccactggacc ctcttggcga ctgatctgtc tgcccgagga acacaccgag      540

aagtggaaga agttcatctt cgtttctaac cactgcatgt ctgacggacg atcctctatc      600

cacttctttc acgacctgcg agatgagctg aacaacatca agacccctcc aaagaagctg      660

gactacattt tcaagtacga agaggactac cagctgctgc gaaagctgcc cgagcctatc      720

gagaaggtga tcgacttccg acctccttac ctgttcatcc ccaagtctct gctgtctgga      780

ttcatctaca accacctccg attctcttcg aagggcgtgt gcatgcgaat ggacgacgtg      840

gaaaagaccg acgacgttgt gaccgagatc atcaacatct ctcccaccga gttccaggcc      900

atcaaggcca acattaagtc taacatccag ggcaagtgta ccatcactcc ctttctgcac      960

gtgtgctggt tcgtgtctct gcacaagtgg ggcaagttct ttaagcccct gaacttcgag     1020

tggctgaccg acatcttcat ccccgccgac tgccgatctc agctgcctga cgacgacgag     1080

atgcgacaga tgtaccgata cggcgccaac gtgggcttca tcgacttcac cccttggatc     1140

tctgagttcg acatgaacga caacaaggaa aacttctggc ccctgatcga gcactaccac     1200

gaggtgattt ctgaggccct gcgaaacaag aagcacctcc acggcctggg cttcaacatt     1260

cagggcttcg tccagaagta cgtcaacatt gacaaggtga tgtgcgaccg agccatcggc     1320

aagcgacgag gcggcaccct gctgtctaac gtgggcctgt tcaaccagct cgaggaaccc     1380

gacgccaagt actctatctg cgacctggcc ttcggccagt tccaaggctc ttggcaccag     1440

gctttctccc tgggcgtgtg ttctaccaac gtgaagggca tgaacatcgt ggtggcctct     1500

accaagaacg tggtgggctc tcaagagtct ctggaagaac tgtgctctat ctacaaggcc     1560

ctgctgctgg gcccctaa                                                   1578


<210>  62
<211>  525
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  62

Met Asn Glu Ile Asp Glu Lys Asn Gln Ala Pro Val Gln Gln Glu Cys 
1               5                   10                  15      


Leu Lys Glu Met Ile Gln Asn Gly His Ala Arg Arg Met Gly Ser Val 
            20                  25                  30          


Glu Asp Leu Tyr Val Ala Leu Asn Arg Gln Asn Leu Tyr Arg Asn Phe 
        35                  40                  45              


Cys Thr Tyr Gly Glu Leu Ser Asp Tyr Cys Thr Arg Asp Gln Leu Thr 
    50                  55                  60                  


Leu Ala Leu Arg Glu Ile Cys Leu Lys Asn Pro Thr Leu Leu His Ile 
65                  70                  75                  80  


Val Leu Pro Thr Arg Trp Pro Asn His Glu Asn Tyr Tyr Arg Ser Ser 
                85                  90                  95      


Glu Tyr Tyr Ser Arg Pro His Pro Val His Asp Tyr Ile Ser Val Leu 
            100                 105                 110         


Gln Glu Leu Lys Leu Ser Gly Val Val Leu Asn Glu Gln Pro Glu Tyr 
        115                 120                 125             


Ser Ala Val Met Lys Gln Ile Leu Glu Glu Phe Lys Asn Ser Lys Gly 
    130                 135                 140                 


Ser Tyr Thr Ala Lys Ile Phe Lys Leu Thr Thr Thr Leu Thr Ile Pro 
145                 150                 155                 160 


Tyr Phe Gly Pro Thr Gly Pro Ser Trp Arg Leu Ile Cys Leu Pro Glu 
                165                 170                 175     


Glu His Thr Glu Lys Trp Lys Lys Phe Ile Phe Val Ser Asn His Cys 
            180                 185                 190         


Met Ser Asp Gly Arg Ser Ser Ile His Phe Phe His Asp Leu Arg Asp 
        195                 200                 205             


Glu Leu Asn Asn Ile Lys Thr Pro Pro Lys Lys Leu Asp Tyr Ile Phe 
    210                 215                 220                 


Lys Tyr Glu Glu Asp Tyr Gln Leu Leu Arg Lys Leu Pro Glu Pro Ile 
225                 230                 235                 240 


Glu Lys Val Ile Asp Phe Arg Pro Pro Tyr Leu Phe Ile Pro Lys Ser 
                245                 250                 255     


Leu Leu Ser Gly Phe Ile Tyr Asn His Leu Arg Phe Ser Ser Lys Gly 
            260                 265                 270         


Val Cys Met Arg Met Asp Asp Val Glu Lys Thr Asp Asp Val Val Thr 
        275                 280                 285             


Glu Ile Ile Asn Ile Ser Pro Thr Glu Phe Gln Ala Ile Lys Ala Asn 
    290                 295                 300                 


Ile Lys Ser Asn Ile Gln Gly Lys Cys Thr Ile Thr Pro Phe Leu His 
305                 310                 315                 320 


Val Cys Trp Phe Val Ser Leu His Lys Trp Gly Lys Phe Phe Lys Pro 
                325                 330                 335     


Leu Asn Phe Glu Trp Leu Thr Asp Ile Phe Ile Pro Ala Asp Cys Arg 
            340                 345                 350         


Ser Gln Leu Pro Asp Asp Asp Glu Met Arg Gln Met Tyr Arg Tyr Gly 
        355                 360                 365             


Ala Asn Val Gly Phe Ile Asp Phe Thr Pro Trp Ile Ser Glu Phe Asp 
    370                 375                 380                 


Met Asn Asp Asn Lys Glu Asn Phe Trp Pro Leu Ile Glu His Tyr His 
385                 390                 395                 400 


Glu Val Ile Ser Glu Ala Leu Arg Asn Lys Lys His Leu His Gly Leu 
                405                 410                 415     


Gly Phe Asn Ile Gln Gly Phe Val Gln Lys Tyr Val Asn Ile Asp Lys 
            420                 425                 430         


Val Met Cys Asp Arg Ala Ile Gly Lys Arg Arg Gly Gly Thr Leu Leu 
        435                 440                 445             


Ser Asn Val Gly Leu Phe Asn Gln Leu Glu Glu Pro Asp Ala Lys Tyr 
    450                 455                 460                 


Ser Ile Cys Asp Leu Ala Phe Gly Gln Phe Gln Gly Ser Trp His Gln 
465                 470                 475                 480 


Ala Phe Ser Leu Gly Val Cys Ser Thr Asn Val Lys Gly Met Asn Ile 
                485                 490                 495     


Val Val Ala Ser Thr Lys Asn Val Val Gly Ser Gln Glu Ser Leu Glu 
            500                 505                 510         


Glu Leu Cys Ser Ile Tyr Lys Ala Leu Leu Leu Gly Pro 
        515                 520                 525 


<210>  63
<211>  1086
<212>  DNA
<213>  Artificial sequence

<220>
<223>  codon-optimized nucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(1086)
<223>  Y. lipolytica codon-optimized nucleotide sequence of delta9 
       desaturase from Drosophila melanogaster Dmd9

<400>  63
atggctccct actctcgaat ctaccaccag gacaagtcgt cccgagagac tggcgtgctg       60

ttcgaggacg acgcccagac cgtggactct gacctgacca ccgaccgatt ccagctgaag      120

cgagccgaga agcgacgact gcccctggtg tggcgaaaca tcatcctgtt cgccctggtg      180

cacctggccg ctctgtacgg cctgcactct atcttcaccc gagccaagct ggccaccact      240

ctgttcgctg ccggcctgta catcatcggc atgctgggcg tgaccgctgg cgcccaccga      300

ctgtgggctc accgaaccta caaggccaag tggcccctgc gactgctgct ggtgatcttc      360

aacaccattg ccttccagga cgccgtgtac cactgggccc gagatcaccg agtgcaccac      420

aagtactctg agactgacgc tgaccctcac aacgctaccc gaggcttctt cttctctcac      480

gtcggctggc tgctgtgcaa gaagcacccc gacatcaagg aaaagggccg aggcctggac      540

ctgtctgacc tgcgagctga ccccatcctg atgttccagc gaaagcacta ctacattctg      600

atgcccctgg cctgcttcgt gctgcccacc gtgattccca tggtgtactg gaacgagact      660

ctggcctctt cctggttcgt ggccaccatg ttccgatggt gcttccagct caacatgacc      720

tggctggtga actctgccgc tcacaagttc ggcaaccgac cttacgacaa gactatgaac      780

cccactcaga acgccttcgt gtctgccttc accttcggcg aaggctggca caactaccac      840

cacgcattcc cttgggacta caagaccgcc gagtggggct gctactctct gaacatcacc      900

accgccttca tcgacctgtt cgctaagatc ggctgggcct acgacctcaa gaccgtggct      960

cccgacgtga tccagcgacg agtgctgcga accggcgacg gctctcacga gctgtggggc     1020

tggggcgaca aggacctgac cgctgaggac gcccgaaacg tcctgctggt ggacaagtct     1080

cgataa                                                                1086


<210>  64
<211>  1368
<212>  DNA
<213>  Artificial sequence

<220>
<223>  codon-optimized nucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(1368)
<223>  Y.lipolytica codon-optimized nucleotide sequence of fatty acyl 
       reductase from H. armigera Har_FAR

<400>  64
atggtggtcc tgacctctaa ggagactaag ccctccgtgg ccgagttcta cgctggcaag       60

tctgtcttca tcaccggcgg aaccggtttc ctgggcaagg tcttcattga gaagctgctg      120

tactcctgtc ccgacatcgg caacatctac atgctgatcc gagagaagaa gggactgtct      180

gtgtccgagc gaattaagca cttcctggac gaccccctgt tcacccgact gaaggagaag      240

cgacccgccg acctggagaa gatcgtgctg attcccggag acatcaccgc tcccgacctg      300

ggtattacct ctgagaacga gaagatgctg atcgagaagg tgtctgtcat cattcactcc      360

gccgctaccg tcaagttcaa cgagcccctg cccaccgcct ggaagatcaa cgtggaggga      420

acccgaatga tgctggctct gtctcgacga atgaagcgaa ttgaggtctt catccacatt      480

tccaccgcct acaccaacac caaccgagag gtggtggacg agatcctgta ccctgctcct      540

gctgacattg accaggtgca ccgatacgtc aaggacggta tctctgagga agagactgag      600

aagattctga acggccgacc caacacctac accttcacca aggccctgac cgagcacctg      660

gtggctgaga accaggctta cgtgcccacc atcattgtcc gaccctccgt ggtcgccgct      720

atcaaggacg agcccattaa gggatggctg ggtaactggt acggagctac cggactgacc      780

gtgttcaccg ctaagggtct gaaccgagtc atctacggcc actcttccaa catcgtggac      840

ctgattcccg tggactacgt cgccaacctg gtcattgccg ctggcgctaa gtcttccaag      900

tccaccgagc tgaaggtgta caactgttgc tcttccgcct gcaaccccat caccattgga      960

aagctgatgt ctatgttcgc cgaggacgct atcaagcaga agtcctacgc tatgcccctg     1020

cccggttggt acatcttcac caagtacaag tggctggtcc tgctgctgac cattctgttc     1080

caggtcatcc ccgcctacat taccgacctg taccgacacc tgatcggcaa gaacccccga     1140

tacattaagc tgcagtctct ggtcaaccag acccgatctt ccattgactt cttcacctct     1200

cactcctggg tcatgaaggc tgaccgagtc cgagagctgt tcgcctctct gtcccccgct     1260

gacaagtacc tgttcccctg tgaccccacc gacatcaact ggacccacta cattcaggac     1320

tactgctggg gagtgcgaca cttcctggag aagaagtcct acgagtag                  1368


<210>  65
<211>  1062
<212>  DNA
<213>  Spodoptera litura

<400>  65
atggctccca acatctctga ggacgtgaac ggcgtgctgt tcgagtctga cgccgctact       60

cccgacctgg ctctggcccg acctcctgtg cagaaggccg acaacaagcc caagcagctg      120

gtgtggcgaa acatcatcct gttcgcctac ctgcacctgg ccgctctgta cggcggctac      180

ctgttcctgt tctctgccaa gtggcagacc gacatcttcg cctacatcct gtacgtgatc      240

tctggcctgg gcatcaccgc tggcgcccac cgactgtggg ctcacaagtc ttacaaggct      300

aagtggcccc tgaaggtgat cctgatcatc ttcaacaccg tggccttcca ggacgccgcc      360

atggactggg cccgagatca ccgaatgcac cacaagtact ctgagactga cgctgaccct      420

cacaacgcta cccgaggctt cttcttctct cacatcggct ggctgctggt gcgaaagcac      480

cccgacctga aggaaaaggg caagggcctc gacatgtctg acctgctggc tgaccccgtg      540

ctgcgattcc agaagaagta ctacctgctg ctgatgcccc tggcctgctt cgtcatgccc      600

accgtgattc ccgtgtacct gtggggcgag acttggacca acgccttctt cgtggccgcc      660

atgttccgat acgccttcat tctgaacgtg acctggctgg tgaactctgc tgcccacaag      720

tggggcgaca agccctacga caagtctatc aagccctctg agaacatgtc tgtggccatg      780

ttcgccctcg gcgagggatt ccacaactac caccacacat tcccttggga ctacaagacc      840

gccgagttcg gcaacaacaa gctgaacttc actaccgcct tcatcaactt tttcgccaag      900

atcggctggg cttacgacat gaagaccgtg tctgaggaca tcgtgaagaa ccgagtgaag      960

cgaaccggcg acggctctca ccacctctgg ggctggggcg acgagaacca gcctaaggaa     1020

gagatcgagg ccgccatccg aatcaacccc aaggacgact aa                        1062


<210>  66
<211>  353
<212>  PRT
<213>  Spodoptera litura

<400>  66

Met Ala Pro Asn Ile Ser Glu Asp Val Asn Gly Val Leu Phe Glu Ser 
1               5                   10                  15      


Asp Ala Ala Thr Pro Asp Leu Ala Leu Ala Arg Pro Pro Val Gln Lys 
            20                  25                  30          


Ala Asp Asn Lys Pro Lys Gln Leu Val Trp Arg Asn Ile Ile Leu Phe 
        35                  40                  45              


Ala Tyr Leu His Leu Ala Ala Leu Tyr Gly Gly Tyr Leu Phe Leu Phe 
    50                  55                  60                  


Ser Ala Lys Trp Gln Thr Asp Ile Phe Ala Tyr Ile Leu Tyr Val Ile 
65                  70                  75                  80  


Ser Gly Leu Gly Ile Thr Ala Gly Ala His Arg Leu Trp Ala His Lys 
                85                  90                  95      


Ser Tyr Lys Ala Lys Trp Pro Leu Lys Val Ile Leu Ile Ile Phe Asn 
            100                 105                 110         


Thr Val Ala Phe Gln Asp Ala Ala Met Asp Trp Ala Arg Asp His Arg 
        115                 120                 125             


Met His His Lys Tyr Ser Glu Thr Asp Ala Asp Pro His Asn Ala Thr 
    130                 135                 140                 


Arg Gly Phe Phe Phe Ser His Ile Gly Trp Leu Leu Val Arg Lys His 
145                 150                 155                 160 


Pro Asp Leu Lys Glu Lys Gly Lys Gly Leu Asp Met Ser Asp Leu Leu 
                165                 170                 175     


Ala Asp Pro Val Leu Arg Phe Gln Lys Lys Tyr Tyr Leu Leu Leu Met 
            180                 185                 190         


Pro Leu Ala Cys Phe Val Met Pro Thr Val Ile Pro Val Tyr Leu Trp 
        195                 200                 205             


Gly Glu Thr Trp Thr Asn Ala Phe Phe Val Ala Ala Met Phe Arg Tyr 
    210                 215                 220                 


Ala Phe Ile Leu Asn Val Thr Trp Leu Val Asn Ser Ala Ala His Lys 
225                 230                 235                 240 


Trp Gly Asp Lys Pro Tyr Asp Lys Ser Ile Lys Pro Ser Glu Asn Met 
                245                 250                 255     


Ser Val Ala Met Phe Ala Leu Gly Glu Gly Phe His Asn Tyr His His 
            260                 265                 270         


Thr Phe Pro Trp Asp Tyr Lys Thr Ala Glu Phe Gly Asn Asn Lys Leu 
        275                 280                 285             


Asn Phe Thr Thr Ala Phe Ile Asn Phe Phe Ala Lys Ile Gly Trp Ala 
    290                 295                 300                 


Tyr Asp Met Lys Thr Val Ser Glu Asp Ile Val Lys Asn Arg Val Lys 
305                 310                 315                 320 


Arg Thr Gly Asp Gly Ser His His Leu Trp Gly Trp Gly Asp Glu Asn 
                325                 330                 335     


Gln Pro Lys Glu Glu Ile Glu Ala Ala Ile Arg Ile Asn Pro Lys Asp 
            340                 345                 350         


Asp 
    


<210>  67
<211>  981
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Codon-optimised sequence


<220>
<221>  CDS
<222>  (1)..(981)
<223>  Y. lipolytica codon-optimized nucleotide sequence of ?11 
       desaturase from Amyelois transitella

<400>  67
atg gtg ccc aac aag ggt tct tcc gac gtc ctg tct gag cac tcc gag         48
Met Val Pro Asn Lys Gly Ser Ser Asp Val Leu Ser Glu His Ser Glu           
1               5                   10                  15                

ccc cag ttc acc aag ctg att gct ccc cag gct ggc ccc cga aag tac         96
Pro Gln Phe Thr Lys Leu Ile Ala Pro Gln Ala Gly Pro Arg Lys Tyr           
            20                  25                  30                    

aag atc gtg tac cga aac ctg ctg acc ttc gga tac tgg cac ctg tct        144
Lys Ile Val Tyr Arg Asn Leu Leu Thr Phe Gly Tyr Trp His Leu Ser           
        35                  40                  45                        

gcc gtc tac ggt ctg tac ctg tgt ttc acc tgc gcc aag tgg gct acc        192
Ala Val Tyr Gly Leu Tyr Leu Cys Phe Thr Cys Ala Lys Trp Ala Thr           
    50                  55                  60                            

att ctg ttc gcc ttc ttc ctg tac gtg atc gct gag atc ggc att acc        240
Ile Leu Phe Ala Phe Phe Leu Tyr Val Ile Ala Glu Ile Gly Ile Thr           
65                  70                  75                  80            

ggc gga gcc cac cga ctg tgg gct cac cga acc tac aag gcc aag ctg        288
Gly Gly Ala His Arg Leu Trp Ala His Arg Thr Tyr Lys Ala Lys Leu           
                85                  90                  95                

ccc ctg gag atc ctg ctg ctg att atg aac tct atc gct ttc cag gac        336
Pro Leu Glu Ile Leu Leu Leu Ile Met Asn Ser Ile Ala Phe Gln Asp           
            100                 105                 110                   

acc gcc ttc acc tgg gct cga gat cac cga ctg cac cac aag tac tct        384
Thr Ala Phe Thr Trp Ala Arg Asp His Arg Leu His His Lys Tyr Ser           
        115                 120                 125                       

gac acc gac gct gac cct cac aac gct acc cga ggt ttc ttc tac tcc        432
Asp Thr Asp Ala Asp Pro His Asn Ala Thr Arg Gly Phe Phe Tyr Ser           
    130                 135                 140                           

cac gtg ggc tgg ctg ctg gtc aag aag cac ccc gag gtg aag gcc cgg        480
His Val Gly Trp Leu Leu Val Lys Lys His Pro Glu Val Lys Ala Arg           
145                 150                 155                 160           

gga aag tac ctg tcc ctg gac gac ctg aag aac aac ccc ctg ctg aag        528
Gly Lys Tyr Leu Ser Leu Asp Asp Leu Lys Asn Asn Pro Leu Leu Lys           
                165                 170                 175               

ttc cag aag aag tac gct atc ctg gtc att ggc acc ctg tgt ttc ctg        576
Phe Gln Lys Lys Tyr Ala Ile Leu Val Ile Gly Thr Leu Cys Phe Leu           
            180                 185                 190                   

atg ccc acc ttc gtg ccc gtc tac ttc tgg ggt gag ggc att tct acc        624
Met Pro Thr Phe Val Pro Val Tyr Phe Trp Gly Glu Gly Ile Ser Thr           
        195                 200                 205                       

gcc tgg aac atc aac ctg ctg cga tac gtg atg aac ctg aac atg acc        672
Ala Trp Asn Ile Asn Leu Leu Arg Tyr Val Met Asn Leu Asn Met Thr           
    210                 215                 220                           

ttc ctg gtc aac tcc gcc gct cac att ttc ggc aac aag ccc tac gac        720
Phe Leu Val Asn Ser Ala Ala His Ile Phe Gly Asn Lys Pro Tyr Asp           
225                 230                 235                 240           

aag tct att gcc tcc gtg cag aac atc tct gtc tcc ctg gct acc ttc        768
Lys Ser Ile Ala Ser Val Gln Asn Ile Ser Val Ser Leu Ala Thr Phe           
                245                 250                 255               

gga gag ggt ttc cac aac tac cac cac acc tac cct tgg gac tac cga        816
Gly Glu Gly Phe His Asn Tyr His His Thr Tyr Pro Trp Asp Tyr Arg           
            260                 265                 270                   

gct gct gag ctg ggc aac aac cga ctg aac atg acc acc gcc ttc att        864
Ala Ala Glu Leu Gly Asn Asn Arg Leu Asn Met Thr Thr Ala Phe Ile           
        275                 280                 285                       

gac ttc ttc gcc tgg atc gga tgg gct tac gac ctg aag tcc gtc ccc        912
Asp Phe Phe Ala Trp Ile Gly Trp Ala Tyr Asp Leu Lys Ser Val Pro           
    290                 295                 300                           

cag gaa gcc atc gct aag cga tgc gct aag acc ggc gac gga acc gac        960
Gln Glu Ala Ile Ala Lys Arg Cys Ala Lys Thr Gly Asp Gly Thr Asp           
305                 310                 315                 320           

atg tgg gga cga aag cga tag                                            981
Met Trp Gly Arg Lys Arg                                                   
                325                                                       


<210>  68
<211>  326
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  68

Met Val Pro Asn Lys Gly Ser Ser Asp Val Leu Ser Glu His Ser Glu 
1               5                   10                  15      


Pro Gln Phe Thr Lys Leu Ile Ala Pro Gln Ala Gly Pro Arg Lys Tyr 
            20                  25                  30          


Lys Ile Val Tyr Arg Asn Leu Leu Thr Phe Gly Tyr Trp His Leu Ser 
        35                  40                  45              


Ala Val Tyr Gly Leu Tyr Leu Cys Phe Thr Cys Ala Lys Trp Ala Thr 
    50                  55                  60                  


Ile Leu Phe Ala Phe Phe Leu Tyr Val Ile Ala Glu Ile Gly Ile Thr 
65                  70                  75                  80  


Gly Gly Ala His Arg Leu Trp Ala His Arg Thr Tyr Lys Ala Lys Leu 
                85                  90                  95      


Pro Leu Glu Ile Leu Leu Leu Ile Met Asn Ser Ile Ala Phe Gln Asp 
            100                 105                 110         


Thr Ala Phe Thr Trp Ala Arg Asp His Arg Leu His His Lys Tyr Ser 
        115                 120                 125             


Asp Thr Asp Ala Asp Pro His Asn Ala Thr Arg Gly Phe Phe Tyr Ser 
    130                 135                 140                 


His Val Gly Trp Leu Leu Val Lys Lys His Pro Glu Val Lys Ala Arg 
145                 150                 155                 160 


Gly Lys Tyr Leu Ser Leu Asp Asp Leu Lys Asn Asn Pro Leu Leu Lys 
                165                 170                 175     


Phe Gln Lys Lys Tyr Ala Ile Leu Val Ile Gly Thr Leu Cys Phe Leu 
            180                 185                 190         


Met Pro Thr Phe Val Pro Val Tyr Phe Trp Gly Glu Gly Ile Ser Thr 
        195                 200                 205             


Ala Trp Asn Ile Asn Leu Leu Arg Tyr Val Met Asn Leu Asn Met Thr 
    210                 215                 220                 


Phe Leu Val Asn Ser Ala Ala His Ile Phe Gly Asn Lys Pro Tyr Asp 
225                 230                 235                 240 


Lys Ser Ile Ala Ser Val Gln Asn Ile Ser Val Ser Leu Ala Thr Phe 
                245                 250                 255     


Gly Glu Gly Phe His Asn Tyr His His Thr Tyr Pro Trp Asp Tyr Arg 
            260                 265                 270         


Ala Ala Glu Leu Gly Asn Asn Arg Leu Asn Met Thr Thr Ala Phe Ile 
        275                 280                 285             


Asp Phe Phe Ala Trp Ile Gly Trp Ala Tyr Asp Leu Lys Ser Val Pro 
    290                 295                 300                 


Gln Glu Ala Ile Ala Lys Arg Cys Ala Lys Thr Gly Asp Gly Thr Asp 
305                 310                 315                 320 


Met Trp Gly Arg Lys Arg 
                325     


<210>  69
<211>  1368
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Codon-optimised sequence


<220>
<221>  CDS
<222>  (1)..(1368)
<223>  Y. lipolytica codon-optimized nucleotide sequence of fatty acyl 
       reductase from Helicoverpa armigera

<400>  69
atg gtg gtc ctg acc tct aag gag act aag ccc tcc gtg gcc gag ttc         48
Met Val Val Leu Thr Ser Lys Glu Thr Lys Pro Ser Val Ala Glu Phe           
1               5                   10                  15                

tac gct ggc aag tct gtc ttc atc acc ggc gga acc ggt ttc ctg ggc         96
Tyr Ala Gly Lys Ser Val Phe Ile Thr Gly Gly Thr Gly Phe Leu Gly           
            20                  25                  30                    

aag gtc ttc att gag aag ctg ctg tac tcc tgt ccc gac atc ggc aac        144
Lys Val Phe Ile Glu Lys Leu Leu Tyr Ser Cys Pro Asp Ile Gly Asn           
        35                  40                  45                        

atc tac atg ctg atc cga gag aag aag gga ctg tct gtg tcc gag cga        192
Ile Tyr Met Leu Ile Arg Glu Lys Lys Gly Leu Ser Val Ser Glu Arg           
    50                  55                  60                            

att aag cac ttc ctg gac gac ccc ctg ttc acc cga ctg aag gag aag        240
Ile Lys His Phe Leu Asp Asp Pro Leu Phe Thr Arg Leu Lys Glu Lys           
65                  70                  75                  80            

cga ccc gcc gac ctg gag aag atc gtg ctg att ccc gga gac atc acc        288
Arg Pro Ala Asp Leu Glu Lys Ile Val Leu Ile Pro Gly Asp Ile Thr           
                85                  90                  95                

gct ccc gac ctg ggt att acc tct gag aac gag aag atg ctg atc gag        336
Ala Pro Asp Leu Gly Ile Thr Ser Glu Asn Glu Lys Met Leu Ile Glu           
            100                 105                 110                   

aag gtg tct gtc atc att cac tcc gcc gct acc gtc aag ttc aac gag        384
Lys Val Ser Val Ile Ile His Ser Ala Ala Thr Val Lys Phe Asn Glu           
        115                 120                 125                       

ccc ctg ccc acc gcc tgg aag atc aac gtg gag gga acc cga atg atg        432
Pro Leu Pro Thr Ala Trp Lys Ile Asn Val Glu Gly Thr Arg Met Met           
    130                 135                 140                           

ctg gct ctg tct cga cga atg aag cga att gag gtc ttc atc cac att        480
Leu Ala Leu Ser Arg Arg Met Lys Arg Ile Glu Val Phe Ile His Ile           
145                 150                 155                 160           

tcc acc gcc tac acc aac acc aac cga gag gtg gtg gac gag atc ctg        528
Ser Thr Ala Tyr Thr Asn Thr Asn Arg Glu Val Val Asp Glu Ile Leu           
                165                 170                 175               

tac cct gct cct gct gac att gac cag gtg cac cga tac gtc aag gac        576
Tyr Pro Ala Pro Ala Asp Ile Asp Gln Val His Arg Tyr Val Lys Asp           
            180                 185                 190                   

ggt atc tct gag gaa gag act gag aag att ctg aac ggc cga ccc aac        624
Gly Ile Ser Glu Glu Glu Thr Glu Lys Ile Leu Asn Gly Arg Pro Asn           
        195                 200                 205                       

acc tac acc ttc acc aag gcc ctg acc gag cac ctg gtg gct gag aac        672
Thr Tyr Thr Phe Thr Lys Ala Leu Thr Glu His Leu Val Ala Glu Asn           
    210                 215                 220                           

cag gct tac gtg ccc acc atc att gtc cga ccc tcc gtg gtc gcc gct        720
Gln Ala Tyr Val Pro Thr Ile Ile Val Arg Pro Ser Val Val Ala Ala           
225                 230                 235                 240           

atc aag gac gag ccc att aag gga tgg ctg ggt aac tgg tac gga gct        768
Ile Lys Asp Glu Pro Ile Lys Gly Trp Leu Gly Asn Trp Tyr Gly Ala           
                245                 250                 255               

acc gga ctg acc gtg ttc acc gct aag ggt ctg aac cga gtc atc tac        816
Thr Gly Leu Thr Val Phe Thr Ala Lys Gly Leu Asn Arg Val Ile Tyr           
            260                 265                 270                   

ggc cac tct tcc aac atc gtg gac ctg att ccc gtg gac tac gtc gcc        864
Gly His Ser Ser Asn Ile Val Asp Leu Ile Pro Val Asp Tyr Val Ala           
        275                 280                 285                       

aac ctg gtc att gcc gct ggc gct aag tct tcc aag tcc acc gag ctg        912
Asn Leu Val Ile Ala Ala Gly Ala Lys Ser Ser Lys Ser Thr Glu Leu           
    290                 295                 300                           

aag gtg tac aac tgt tgc tct tcc gcc tgc aac ccc atc acc att gga        960
Lys Val Tyr Asn Cys Cys Ser Ser Ala Cys Asn Pro Ile Thr Ile Gly           
305                 310                 315                 320           

aag ctg atg tct atg ttc gcc gag gac gct atc aag cag aag tcc tac       1008
Lys Leu Met Ser Met Phe Ala Glu Asp Ala Ile Lys Gln Lys Ser Tyr           
                325                 330                 335               

gct atg ccc ctg ccc ggt tgg tac atc ttc acc aag tac aag tgg ctg       1056
Ala Met Pro Leu Pro Gly Trp Tyr Ile Phe Thr Lys Tyr Lys Trp Leu           
            340                 345                 350                   

gtc ctg ctg ctg acc att ctg ttc cag gtc atc ccc gcc tac att acc       1104
Val Leu Leu Leu Thr Ile Leu Phe Gln Val Ile Pro Ala Tyr Ile Thr           
        355                 360                 365                       

gac ctg tac cga cac ctg atc ggc aag aac ccc cga tac att aag ctg       1152
Asp Leu Tyr Arg His Leu Ile Gly Lys Asn Pro Arg Tyr Ile Lys Leu           
    370                 375                 380                           

cag tct ctg gtc aac cag acc cga tct tcc att gac ttc ttc acc tct       1200
Gln Ser Leu Val Asn Gln Thr Arg Ser Ser Ile Asp Phe Phe Thr Ser           
385                 390                 395                 400           

cac tcc tgg gtc atg aag gct gac cga gtc cga gag ctg ttc gcc tct       1248
His Ser Trp Val Met Lys Ala Asp Arg Val Arg Glu Leu Phe Ala Ser           
                405                 410                 415               

ctg tcc ccc gct gac aag tac ctg ttc ccc tgt gac ccc acc gac atc       1296
Leu Ser Pro Ala Asp Lys Tyr Leu Phe Pro Cys Asp Pro Thr Asp Ile           
            420                 425                 430                   

aac tgg acc cac tac att cag gac tac tgc tgg gga gtg cga cac ttc       1344
Asn Trp Thr His Tyr Ile Gln Asp Tyr Cys Trp Gly Val Arg His Phe           
        435                 440                 445                       

ctg gag aag aag tcc tac gag tag                                       1368
Leu Glu Lys Lys Ser Tyr Glu                                               
    450                 455                                               


<210>  70
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  70

Met Val Val Leu Thr Ser Lys Glu Thr Lys Pro Ser Val Ala Glu Phe 
1               5                   10                  15      


Tyr Ala Gly Lys Ser Val Phe Ile Thr Gly Gly Thr Gly Phe Leu Gly 
            20                  25                  30          


Lys Val Phe Ile Glu Lys Leu Leu Tyr Ser Cys Pro Asp Ile Gly Asn 
        35                  40                  45              


Ile Tyr Met Leu Ile Arg Glu Lys Lys Gly Leu Ser Val Ser Glu Arg 
    50                  55                  60                  


Ile Lys His Phe Leu Asp Asp Pro Leu Phe Thr Arg Leu Lys Glu Lys 
65                  70                  75                  80  


Arg Pro Ala Asp Leu Glu Lys Ile Val Leu Ile Pro Gly Asp Ile Thr 
                85                  90                  95      


Ala Pro Asp Leu Gly Ile Thr Ser Glu Asn Glu Lys Met Leu Ile Glu 
            100                 105                 110         


Lys Val Ser Val Ile Ile His Ser Ala Ala Thr Val Lys Phe Asn Glu 
        115                 120                 125             


Pro Leu Pro Thr Ala Trp Lys Ile Asn Val Glu Gly Thr Arg Met Met 
    130                 135                 140                 


Leu Ala Leu Ser Arg Arg Met Lys Arg Ile Glu Val Phe Ile His Ile 
145                 150                 155                 160 


Ser Thr Ala Tyr Thr Asn Thr Asn Arg Glu Val Val Asp Glu Ile Leu 
                165                 170                 175     


Tyr Pro Ala Pro Ala Asp Ile Asp Gln Val His Arg Tyr Val Lys Asp 
            180                 185                 190         


Gly Ile Ser Glu Glu Glu Thr Glu Lys Ile Leu Asn Gly Arg Pro Asn 
        195                 200                 205             


Thr Tyr Thr Phe Thr Lys Ala Leu Thr Glu His Leu Val Ala Glu Asn 
    210                 215                 220                 


Gln Ala Tyr Val Pro Thr Ile Ile Val Arg Pro Ser Val Val Ala Ala 
225                 230                 235                 240 


Ile Lys Asp Glu Pro Ile Lys Gly Trp Leu Gly Asn Trp Tyr Gly Ala 
                245                 250                 255     


Thr Gly Leu Thr Val Phe Thr Ala Lys Gly Leu Asn Arg Val Ile Tyr 
            260                 265                 270         


Gly His Ser Ser Asn Ile Val Asp Leu Ile Pro Val Asp Tyr Val Ala 
        275                 280                 285             


Asn Leu Val Ile Ala Ala Gly Ala Lys Ser Ser Lys Ser Thr Glu Leu 
    290                 295                 300                 


Lys Val Tyr Asn Cys Cys Ser Ser Ala Cys Asn Pro Ile Thr Ile Gly 
305                 310                 315                 320 


Lys Leu Met Ser Met Phe Ala Glu Asp Ala Ile Lys Gln Lys Ser Tyr 
                325                 330                 335     


Ala Met Pro Leu Pro Gly Trp Tyr Ile Phe Thr Lys Tyr Lys Trp Leu 
            340                 345                 350         


Val Leu Leu Leu Thr Ile Leu Phe Gln Val Ile Pro Ala Tyr Ile Thr 
        355                 360                 365             


Asp Leu Tyr Arg His Leu Ile Gly Lys Asn Pro Arg Tyr Ile Lys Leu 
    370                 375                 380                 


Gln Ser Leu Val Asn Gln Thr Arg Ser Ser Ile Asp Phe Phe Thr Ser 
385                 390                 395                 400 


His Ser Trp Val Met Lys Ala Asp Arg Val Arg Glu Leu Phe Ala Ser 
                405                 410                 415     


Leu Ser Pro Ala Asp Lys Tyr Leu Phe Pro Cys Asp Pro Thr Asp Ile 
            420                 425                 430         


Asn Trp Thr His Tyr Ile Gln Asp Tyr Cys Trp Gly Val Arg His Phe 
        435                 440                 445             


Leu Glu Lys Lys Ser Tyr Glu 
    450                 455 


<210>  71
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  PR-10714

<400>  71
ttgcttgcga acctaattcc                                                   20


<210>  72
<211>  51
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(51)
<223>  PR-10766

<400>  72
ataacttcgu ataatgtatg ctatacgaag ttataaggag tttggcgccc g                51


<210>  73
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  PR-10767

<400>  73
tcatggacat ggcatagac                                                    19


<210>  74
<211>  51
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(51)
<223>  PR-11047

<400>  74
aataacttcg uatagcatac attatacgaa gttatcgagc gtcccaaaac c                51


<210>  75
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  PR-13338

<400>  75
acctgcacug ttgatgtgtg tttaattc                                          28


<210>  76
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(22)
<223>  PR-141

<400>  76
agaacagcug aagcttcgta cg                                                22


<210>  77
<211>  31
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(31)
<223>  PR-14148

<400>  77
acgtgcaacg ctuacgcaac taacatgaat g                                      31


<210>  78
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  PR-14149

<400>  78
tgacttcaac attatatcgc tctga                                             25


<210>  79
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  PR-142

<400>  79
aggccacuag tggatctgat atcac                                             25


<210>  80
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  PR-14565

<400>  80
agtggccugg ggctggcgtg tgaaggag                                          28


<210>  81
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(27)
<223>  PR-14566

<400>  81
acgcgaucag acctctcaca cggcatc                                           27


<210>  82
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  PR-14567

<400>  82
taatacgact cactataggg c                                                 21


<210>  83
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  PR-14568

<400>  83
cacgcgaucc ttgagacgtt accagagc                                          28


<210>  84
<211>  37
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(37)
<223>  PR-14589

<400>  84
cgtgcgaugc ggaggagcaa tagacatacg atttgac                                37


<210>  85
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<223>  PR-14590

<400>  85
aagcgttgca cgutcccctc cccacggtg                                         29


<210>  86
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  PR-14591

<400>  86
agtggccuca ccgagggata gggaacac                                          28


<210>  87
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(27)
<223>  PR-14592

<400>  87
acgcgautta acactggacc gtactgc                                           27


<210>  88
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(23)
<223>  PR-15430

<400>  88
cgtgcgaugg ttctatacga tgg                                               23


<210>  89
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  PR-15431

<400>  89
agctgttcut atgatatttt tactaacc                                          28


<210>  90
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(29)
<223>  PR-15432

<400>  90
agtggccuga ttggcgttgt gttcaaatg                                         29


<210>  91
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  PR-15433

<400>  91
cacgcgautt gctcattcac cagaaaag                                          28


<210>  92
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  PR-15434

<400>  92
cgtgcgauat gtacaagtat ttctattg                                          28


<210>  93
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(29)
<223>  PR-15435

<400>  93
agctgttcug aatgataaag agataacag                                         29


<210>  94
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  PR-15436

<400>  94
agtggccutg tggcggaagt tgtacacc                                          28


<210>  95
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  PR-15437

<400>  95
acctgcggtu agtactgcaa aaagtgctgg                                        30


<210>  96
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  PR-15930

<400>  96
acctgcggtu agtactgcaa aaagtgctgg                                        30


<210>  97
<211>  35
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(35)
<223>  PR-16592

<400>  97
agtgcaggug ccacaatggt gcccaacaag ggttc                                  35


<210>  98
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(27)
<223>  PR-16593

<400>  98
cgtgcgauct atcgctttcg tccccac                                           27


<210>  99
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(26)
<223>  PR-16594

<400>  99
aaccgcaggu ggtcctgacc tctaag                                            26


<210>  100
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(29)
<223>  PR-16595

<400>  100
cacgcgauct actcgtagga cttcttctc                                         29


<210>  101
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  PR-14279

<400>  101
cgtgcgauag agaccgggtt                                                   20


<210>  102
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  PR-16618

<400>  102
agcctgcggu tagtactgca aaaagtgctg                                        30


<210>  103
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(27)
<223>  PR-14395

<400>  103
cgtgcgautt gatatggtgt aacaatg                                           27


<210>  104
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  PR-14396

<400>  104
aagcgttgca cguaagcact atcctctgct gcg                                    33


<210>  105
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  PR-14397

<400>  105
agtggccucc gagcgtcgac aagcatac                                          28


<210>  106
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  PR-14398

<400>  106
cacgcgaugt tagaagcaat tggagaag                                          28


<210>  107
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(27)
<223>  PR-15521

<400>  107
cgtgcgauaa ggagtttggc gcccgtt                                           27


<210>  108
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  PR-15522

<400>  108
atgacagaut gctgtagata tgtcttgt                                          28


<210>  109
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(29)
<223>  PR-18486

<400>  109
agtgcaggug ccacaatgaa cgagatc                                           27


<210>  110
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  PR-18487

<400>  110
cgtgcgautt aggggcccag                                                   20


<210>  111
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(23)
<223>  PR-18490

<400>  111
accgcaggcu ccctactctc gaa                                               23


<210>  112
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  PR-18489

<400>  112
cacgcgautt atcgagactt gtcc                                              24


<210>  113
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  PR-18499

<400>  113
atctgtcaug ccacaatggc tccca                                             25


<210>  114
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  PR-18500

<400>  114
cacgcgautt agtcgtcctt gggg                                              24


