                         SEQUENCE LISTING

<110>  UPMC
       CNRS
       INSERM
       Institut Pasteur de Tunis
 
<120>  Lebecetin, a C-type lectin, as neovascularization inhibitor

<130>  B2383PC

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  156
<212>  PRT
<213>  Macrovipera lebetina

<400>  1

Met Gly Arg Ser Ile Ser Val Ser Phe Gly Leu Leu Val Val Phe Leu 
1               5                   10                  15      


Ser Leu Ser Gly Thr Gly Ala Asp Gln Asp Cys Leu Pro Gly Trp Ser 
            20                  25                  30          


Ser His Glu Gly His Cys Tyr Lys Val Phe Asn Leu Asp Lys Thr Trp 
        35                  40                  45              


Glu Asp Ala Glu Lys Phe Cys Thr Glu Gln Ala Asn Ser Gly His Leu 
    50                  55                  60                  


Val Ser Ile Asp Ser Lys Lys Glu Ala Asn Phe Val Ala Glu Leu Val 
65                  70                  75                  80  


Ser Gln Asn Ile Lys Glu Thr Arg Arg Thr Asp Phe Val Trp Ile Gly 
                85                  90                  95      


Leu Arg Ala Glu Asp Lys Arg Gln His Cys Ser Ser Glu Trp Ser Asp 
            100                 105                 110         


Gly Ser Ser Ile Asn Tyr Gln Asn Trp Ile Glu Ala Glu Ser Lys Lys 
        115                 120                 125             


Cys Leu Gly Leu Glu Lys Gln Thr Arg Tyr Arg Lys Trp Val Asn Leu 
    130                 135                 140                 


Asn Cys Gly Gln Pro Tyr Arg Phe Thr Cys Glu Ile 
145                 150                 155     


<210>  2
<211>  133
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Lebecetin alpha subunit without peptide signal sequence

<400>  2

Asp Gln Asp Cys Leu Pro Gly Trp Ser Ser His Glu Gly His Cys Tyr 
1               5                   10                  15      


Lys Val Phe Asn Leu Asp Lys Thr Trp Glu Asp Ala Glu Lys Phe Cys 
            20                  25                  30          


Thr Glu Gln Ala Asn Ser Gly His Leu Val Ser Ile Asp Ser Lys Lys 
        35                  40                  45              


Glu Ala Asn Phe Val Ala Glu Leu Val Ser Gln Asn Ile Lys Glu Thr 
    50                  55                  60                  


Arg Arg Thr Asp Phe Val Trp Ile Gly Leu Arg Ala Glu Asp Lys Arg 
65                  70                  75                  80  


Gln His Cys Ser Ser Glu Trp Ser Asp Gly Ser Ser Ile Asn Tyr Gln 
                85                  90                  95      


Asn Trp Ile Glu Ala Glu Ser Lys Lys Cys Leu Gly Leu Glu Lys Gln 
            100                 105                 110         


Thr Arg Tyr Arg Lys Trp Val Asn Leu Asn Cys Gly Gln Pro Tyr Arg 
        115                 120                 125             


Phe Thr Cys Glu Ile 
    130             


<210>  3
<211>  148
<212>  PRT
<213>  Macrovipera lebetina

<400>  3

Met Gly Arg Ile Ile Phe Val Ser Phe Gly Leu Leu Val Val Phe Leu 
1               5                   10                  15      


Ser Leu Ser Gly Thr Gly Ala Ala Leu Asn Cys Ala Ser Gly Trp Ser 
            20                  25                  30          


Gly Tyr Asp Gln His Cys Tyr Lys Val Phe Asp Lys Pro Lys Ser Trp 
        35                  40                  45              


Ala Asp Ala Glu Lys Phe Cys Lys Lys Gln Thr Ser Gly Gly His Leu 
    50                  55                  60                  


Val Ser Phe His Ser Ser Glu Glu Thr Asp Phe Val Val Glu Leu Val 
65                  70                  75                  80  


Ser Gln Thr Leu Glu Ser Gln Ile Leu Trp Met Gly Leu Ser Lys Val 
                85                  90                  95      


Trp Asn Gln Cys Asp Trp Gly Trp Ser Asn Gly Ala Lys Leu Lys Tyr 
            100                 105                 110         


Lys Ala Trp Ala Glu Glu Ser Tyr Cys Val Tyr Phe Ser Ser Thr Lys 
        115                 120                 125             


Lys Gly Trp Arg Ser Arg Ala Cys Arg Leu Leu Gly His Phe Val Cys 
    130                 135                 140                 


Lys Ser Pro Ala 
145             


<210>  4
<211>  114
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Lebecetin beta subunit without peptide signal sequence

<400>  4

Asp Gln His Cys Tyr Lys Val Phe Asp Lys Pro Lys Ser Trp Ala Asp 
1               5                   10                  15      


Ala Glu Lys Phe Cys Lys Lys Gln Thr Ser Gly Gly His Leu Val Ser 
            20                  25                  30          


Phe His Ser Ser Glu Glu Thr Asp Phe Val Val Glu Leu Val Ser Gln 
        35                  40                  45              


Thr Leu Glu Ser Gln Ile Leu Trp Met Gly Leu Ser Lys Val Trp Asn 
    50                  55                  60                  


Gln Cys Asp Trp Gly Trp Ser Asn Gly Ala Lys Leu Lys Tyr Lys Ala 
65                  70                  75                  80  


Trp Ala Glu Glu Ser Tyr Cys Val Tyr Phe Ser Ser Thr Lys Lys Gly 
                85                  90                  95      


Trp Arg Ser Arg Ala Cys Arg Leu Leu Gly His Phe Val Cys Lys Ser 
            100                 105                 110         


Pro Ala 
        


<210>  5
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Sense primer for GAPDH amplification

<400>  5
acggccgcat cttcttgtgc a                                                 21


<210>  6
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Antisense primer for GAPDH amplification

<400>  6
caggcgccca atacggccaa                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Sense primer for ITGAV amplification

<400>  7
caccctcaga gagggagatg                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Antisense primer for ITGAV amplification

<400>  8
acgtacagga ttgcgctctt                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Sense primer for ITGA5 amplification

<400>  9
agtacgcacc ttgccgctca                                                   20


<210>  10
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Antisense primer for ITGA5 amplification

<400>  10
acacggccag tcttggtgaa c                                                 21


<210>  11
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Sense primer for ITGB3 amplification

<400>  11
aaccggggaa cgctccatga                                                   20


<210>  12
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Antisense primer for ITGB3 amplification

<400>  12
cggcgttttt gccagtatcc g                                                 21


<210>  13
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Sense primer for ITGB5 amplification

<400>  13
agcctttggg gagacgtgtg a                                                 21


<210>  14
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Antisense primer for ITGB5 amplification

<400>  14
tggtggtggc aggtctggtt                                                   20


<210>  15
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lebecetin alpha subunit with peptide signal sequence

<400>  15

Met Ala Cys Pro Gly Phe Leu Trp Ala Leu Val Ile Ser Thr Cys Leu 
1               5                   10                  15      


Glu Phe Ser Met Ala Asp Gln Asp Cys Leu Pro Gly Trp Ser Ser His 
            20                  25                  30          


Glu Gly His Cys Tyr Lys Val Phe Asn Leu Asp Lys Thr Trp Glu Asp 
        35                  40                  45              


Ala Glu Lys Phe Cys Thr Glu Gln Ala Asn Ser Gly His Leu Val Ser 
    50                  55                  60                  


Ile Asp Ser Lys Lys Glu Ala Asn Phe Val Ala Glu Leu Val Ser Gln 
65                  70                  75                  80  


Asn Ile Lys Glu Thr Arg Arg Thr Asp Phe Val Trp Ile Gly Leu Arg 
                85                  90                  95      


Ala Glu Asp Lys Arg Gln His Cys Ser Ser Glu Trp Ser Asp Gly Ser 
            100                 105                 110         


Ser Ile Asn Tyr Gln Asn Trp Ile Glu Ala Glu Ser Lys Lys Cys Leu 
        115                 120                 125             


Gly Leu Glu Lys Gln Thr Arg Tyr Arg Lys Trp Val Asn Leu Asn Cys 
    130                 135                 140                 


Gly Gln Pro Tyr Arg Phe Thr Cys Glu Ile 
145                 150                 


<210>  16
<211>  146
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lebecetin beta subunit with peptide signal sequence

<400>  16

Met Ala Cys Pro Gly Phe Leu Trp Ala Leu Val Ile Ser Thr Cys Leu 
1               5                   10                  15      


Glu Phe Ser Met Ala Ala Leu Asn Cys Ala Ser Gly Trp Ser Gly Tyr 
            20                  25                  30          


Asp Gln His Cys Tyr Lys Val Phe Asp Lys Pro Lys Ser Trp Ala Asp 
        35                  40                  45              


Ala Glu Lys Phe Cys Lys Lys Gln Thr Ser Gly Gly His Leu Val Ser 
    50                  55                  60                  


Phe His Ser Ser Glu Glu Thr Asp Phe Val Val Lys Leu Val Ser Gln 
65                  70                  75                  80  


Thr Leu Glu Ser Gln Ile Leu Trp Met Gly Leu Ser Lys Val Trp Asn 
                85                  90                  95      


Gln Cys Asp Trp Gly Trp Ser Asn Gly Ala Lys Leu Lys Tyr Lys Ala 
            100                 105                 110         


Trp Ala Glu Glu Ser Tyr Cys Val Tyr Phe Ser Ser Thr Lys Lys Gly 
        115                 120                 125             


Trp Arg Ser Arg Ala Cys Arg Leu Leu Gly His Phe Val Cys Lys Ser 
    130                 135                 140                 


Pro Ala 
145     


<210>  17
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide signal sequence

<400>  17

Met Ala Cys Pro Gly Phe Leu Trp Ala Leu Val Ile Ser Thr Cys Leu 
1               5                   10                  15      


Glu Phe Ser Met Ala 
            20      


<210>  18
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lebecetin beta subunit without  peptide signal sequence

<400>  18

Ala Leu Asn Cys Ala Ser Gly Trp Ser Gly Tyr Asp Gln His Cys Tyr 
1               5                   10                  15      


Lys Val Phe Asp Lys Pro Lys Ser Trp Ala Asp Ala Glu Lys Phe Cys 
            20                  25                  30          


Lys Lys Gln Thr Ser Gly Gly His Leu Val Ser Phe His Ser Ser Glu 
        35                  40                  45              


Glu Thr Asp Phe Val Val Lys Leu Val Ser Gln Thr Leu Glu Ser Gln 
    50                  55                  60                  


Ile Leu Trp Met Gly Leu Ser Lys Val Trp Asn Gln Cys Asp Trp Gly 
65                  70                  75                  80  


Trp Ser Asn Gly Ala Lys Leu Lys Tyr Lys Ala Trp Ala Glu Glu Ser 
                85                  90                  95      


Tyr Cys Val Tyr Phe Ser Ser Thr Lys Lys Gly Trp Arg Ser Arg Ala 
            100                 105                 110         


Cys Arg Leu Leu Gly His Phe Val Cys Lys Ser Pro Ala 
        115                 120                 125 


