                               SEQUENCE LISTING

<110> PRESIDENT AND FELLOWS OF HARVARD COLLEGE
 
<120> PROCESSIVE TEMPLATE INDEPENDENT DNA POLYMERASE VARIANTS

<130> 010498.01019/WO

<140> PCT/US2017/064517
<141> 2017-12-04

<150> 62/429,344
<151> 2016-12-02

<160> 3     

<170> PatentIn version 3.5

<210> 1
<211> 755
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 1
Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro 
1               5                   10                  15      


Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu 
            20                  25                  30          


Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu 
        35                  40                  45              


Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys 
    50                  55                  60                  


Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn 
65                  70                  75                  80  


Met Leu Gly Gly Ser Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu 
                85                  90                  95      


Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser 
            100                 105                 110         


Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu 
        115                 120                 125             


Met Leu Lys Met Phe Glu Asp Arg Leu Ser His Lys Thr Tyr Leu Asn 
    130                 135                 140                 


Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp 
145                 150                 155                 160 


Val Val Leu Tyr Met Asp Pro Met Ser Leu Asp Ala Phe Pro Lys Leu 
                165                 170                 175     


Val Ser Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr 
            180                 185                 190         


Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala 
        195                 200                 205             


Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Leu Val Pro Arg 
    210                 215                 220                 


Gly Ser Pro Glu Phe Pro Gly Arg Leu Glu Arg Pro His Arg Asp Gly 
225                 230                 235                 240 


Gly Gly Ser Glu Asn Leu Tyr Phe Gln Gly Ser Gly Gly Ala Ser Arg 
                245                 250                 255     


Gly Val Asn Lys Val Ile Leu Val Gly Asn Leu Gly Gln Asp Pro Glu 
            260                 265                 270         


Val Arg Tyr Met Pro Asn Gly Gly Ala Val Ala Asn Ile Thr Leu Ala 
        275                 280                 285             


Thr Ser Glu Ser Trp Arg Asp Lys Ala Thr Gly Glu Met Lys Glu Gln 
    290                 295                 300                 


Thr Glu Trp His Arg Val Val Leu Phe Gly Lys Leu Ala Glu Val Ala 
305                 310                 315                 320 


Ser Glu Tyr Leu Arg Lys Gly Ser Gln Val Tyr Ile Glu Gly Gln Leu 
                325                 330                 335     


Arg Thr Arg Lys Trp Thr Asp Gln Ser Gly Gln Asp Arg Tyr Thr Thr 
            340                 345                 350         


Glu Val Val Val Asn Val Gly Gly Thr Met Gln Met Leu Gln Leu Gly 
        355                 360                 365             


Thr Gln Pro Glu Leu Ile Gln Asp Ala Gly Gly Gly Val Arg Met Ser 
    370                 375                 380                 


Gly Ala Gly Thr Ala Ser Arg Gly Val Asn Lys Val Ile Leu Val Gly 
385                 390                 395                 400 


Asn Leu Gly Gln Asp Pro Glu Val Arg Tyr Met Pro Asn Gly Gly Ala 
                405                 410                 415     


Val Ala Asn Ile Thr Leu Ala Thr Ser Glu Ser Trp Arg Asp Lys Ala 
            420                 425                 430         


Thr Gly Glu Met Lys Glu Gln Thr Glu Trp His Arg Val Val Leu Phe 
        435                 440                 445             


Gly Lys Leu Ala Glu Val Ala Ser Glu Tyr Leu Arg Lys Gly Ser Gln 
    450                 455                 460                 


Val Tyr Ile Glu Gly Gln Leu Arg Thr Arg Lys Trp Thr Asp Gln Ser 
465                 470                 475                 480 


Gly Gln Asp Arg Tyr Thr Thr Glu Val Val Val Asn Val Gly Gly Thr 
                485                 490                 495     


Met Gln Met Leu Ala Ser His Met Ala Ser Arg Gly Val Asn Lys Val 
            500                 505                 510         


Ile Leu Val Gly Asn Leu Gly Gln Asp Pro Glu Val Arg Tyr Met Pro 
        515                 520                 525             


Asn Gly Gly Ala Val Ala Asn Ile Thr Leu Ala Thr Ser Glu Ser Trp 
    530                 535                 540                 


Arg Asp Lys Ala Thr Gly Glu Met Lys Glu Gln Thr Glu Trp His Arg 
545                 550                 555                 560 


Val Val Leu Phe Gly Lys Leu Ala Glu Val Ala Ser Glu Tyr Leu Arg 
                565                 570                 575     


Lys Gly Ser Gln Val Tyr Ile Glu Gly Gln Leu Arg Thr Arg Lys Trp 
            580                 585                 590         


Thr Asp Gln Ser Gly Gln Asp Arg Tyr Thr Thr Glu Val Val Val Asn 
        595                 600                 605             


Val Gly Gly Thr Met Gln Met Leu Gln Leu Gly Thr Gln Pro Glu Leu 
    610                 615                 620                 


Ile Gln Asp Ala Gly Gly Gly Val Arg Met Ser Gly Ala Gly Thr Ala 
625                 630                 635                 640 


Ser Arg Gly Val Asn Lys Val Ile Leu Val Gly Asn Leu Gly Gln Asp 
                645                 650                 655     


Pro Glu Val Arg Tyr Met Pro Asn Gly Gly Ala Val Ala Asn Ile Thr 
            660                 665                 670         


Leu Ala Thr Ser Glu Ser Trp Arg Asp Lys Ala Thr Gly Glu Met Lys 
        675                 680                 685             


Glu Gln Thr Glu Trp His Arg Val Val Leu Phe Gly Lys Leu Ala Glu 
    690                 695                 700                 


Val Ala Ser Glu Tyr Leu Arg Lys Gly Ser Gln Val Tyr Ile Glu Gly 
705                 710                 715                 720 


Gln Leu Arg Thr Arg Lys Trp Thr Asp Gln Ser Gly Gln Asp Arg Tyr 
                725                 730                 735     


Thr Thr Glu Val Val Val Asn Val Gly Gly Thr Met Gln Met Leu Gly 
            740                 745                 750         


Gly Ser Cys 
        755 


<210> 2
<211> 400
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 2
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Ser Pro Ser Pro Val Pro Gly Ser Gln Asn Val 
            20                  25                  30          


Pro Ala Pro Ala Val Lys Lys Ile Ser Gln Tyr Ala Ala Gln Arg Arg 
        35                  40                  45              


Thr Thr Leu Asn Asn Tyr Asn Gln Leu Phe Thr Asp Ala Leu Asp Ile 
    50                  55                  60                  


Leu Ala Glu Asn Asp Glu Leu Arg Glu Asn Glu Gly Ser Ala Leu Ala 
65                  70                  75                  80  


Phe Met Arg Ala Ser Ser Val Leu Lys Ser Leu Pro Phe Pro Ile Thr 
                85                  90                  95      


Ser Met Lys Asp Thr Glu Gly Ile Pro Asn Leu Gly Asp Lys Val Lys 
            100                 105                 110         


Ser Ile Ile Glu Gly Ile Ile Glu Asp Gly Glu Ser Ser Glu Ala Lys 
        115                 120                 125             


Ala Val Leu Asn Asp Glu Arg Tyr Lys Ser Phe Lys Leu Phe Thr Ser 
    130                 135                 140                 


Val Phe Gly Val Gly Leu Lys Thr Ala Glu Lys Trp Phe Arg Met Gly 
145                 150                 155                 160 


Phe Arg Thr Leu Ser Lys Ile Gln Ser Asp Lys Ser Leu Arg Phe Thr 
                165                 170                 175     


Gln Met Gln Lys Ala Gly Phe Leu Tyr Tyr Glu Asp Leu Val Ser Ala 
            180                 185                 190         


Val Asn Arg Pro Glu Ala Glu Ala Val Ser Met Leu Val Lys Glu Ala 
        195                 200                 205             


Val Val Thr Phe Leu Pro Asp Ala Leu Val Thr Met Thr Gly Gly Phe 
    210                 215                 220                 


Arg Arg Gly Lys Met Thr Gly His Asp Val Asp Phe Leu Ile Thr Ser 
225                 230                 235                 240 


Pro Glu Ala Thr Glu Asp Glu Glu Gln Gln Leu Leu His Lys Val Thr 
                245                 250                 255     


Asp Phe Trp Lys Gln Gln Gly Leu Leu Leu Tyr Gly Asp Ile Leu Glu 
            260                 265                 270         


Ser Thr Phe Glu Lys Phe Lys Gln Pro Ser Arg Lys Val Asp Ala Leu 
        275                 280                 285             


Asp His Phe Gln Lys Ala Phe Leu Ile Leu Lys Leu Asp His Gly Arg 
    290                 295                 300                 


Val His Ser Glu Lys Ser Gly Gln Gln Glu Gly Lys Gly Trp Lys Ala 
305                 310                 315                 320 


Ile Arg Val Asp Leu Val Met Ala Pro Tyr Asp Arg Arg Ala Phe Ala 
                325                 330                 335     


Leu Leu Gly Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Leu Arg Arg 
            340                 345                 350         


Tyr Ala Thr His Glu Arg Lys Met Met Leu Asp Asn His Ala Leu Tyr 
        355                 360                 365             


Asp Arg Thr Lys Arg Val Phe Leu Glu Ala Glu Ser Glu Glu Glu Ile 
    370                 375                 380                 


Phe Ala His Leu Gly Leu Asp Tyr Ile Glu Pro Trp Glu Arg Asn Ala 
385                 390                 395                 400 


<210> 3
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 3
tttttttttt tttttttt                                                     18


