                         SEQUENCE LISTING

<110>  INTERNATIONAL CROPS RESEARCH INSTITUTE FOR THE SEMI_ARID 
       TROPICS (ICRISAT)
 
<120>  CYTOPLASMIC MALE STERILE GENE ORF147 OF PIGEON PEA, AND USES 
       THEREOF

<130>  PD022598IN-SC

<160>  46    

<170>  PatentIn version 3.5

<210>  1
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  orf147 Polypeptide

<400>  1

Met His Leu Val Leu Ser Phe Phe Pro Val Cys Arg Ser Ala Ser Lys 
1               5                   10                  15      


Glu Arg Lys Leu Lys Ala Asn Lys Asp Lys Met Thr Arg Glu Ile Lys 
            20                  25                  30          


Leu Tyr Val Asp Thr Thr Pro Ser Asp Leu Asp Phe Met Met Asn Ser 
        35                  40                  45              


Asp Thr Asp Leu Gln Ser Leu Ser Ser Pro Asp Ser Ser Asp Ala Gln 
    50                  55                  60                  


Ser Ala Ser Pro Asp Leu Asp Leu Leu Trp Asp Gln Val Cys Gly Glu 
65                  70                  75                  80  


Tyr His Lys Cys Val His Glu Ser Gly Arg Val Leu Pro Pro Glu Trp 
                85                  90                  95      


Thr Met Pro Asp Leu Val Arg Ala Val Ile Ser Asp Asp Glu Ala Ile 
            100                 105                 110         


Glu Gln Gly Phe Leu Thr Asp Ala Tyr Tyr Asp Val Met Leu Cys Gly 
        115                 120                 125             


Thr His Ser Trp Val Cys Glu Glu Leu Leu Asn Phe Leu Asp Leu Ile 
    130                 135                 140                 


His Tyr Gly 
145         


<210>  2
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mitochondrial transit peptide

<400>  2

Met Leu Ser Leu Arg Gln Ser Ile Arg Phe Phe Lys Pro Ala Thr Arg 
1               5                   10                  15      


Thr Leu Cys Ser Ser Arg Tyr Leu Leu Gln Gln Lys Pro 
            20                  25                  


<210>  3
<211>  444
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  orf17 DNA

<400>  3
atgcatctgg ttctatcttt ttttccggta tgccgctccg ccagcaagga gcgaaaacta       60

aaagcaaaca aagataagat gaccagagag atcaagctat atgtggatac cacccctagt      120

gatttggatt ttatgatgaa tagtgatacg gatttgcagt ccttgtcttc cccggattcg      180

tccgacgcac agagtgcttc accggacttg gacctattat gggatcaagt ttgtggtgaa      240

taccacaagt gtgtgcatga atccgggagg gtcttacccc cggaatggac gatgcccgac      300

cttgttcggg ctgttatttc cgacgatgaa gctattgagc agggctttct gacggatgcc      360

tactatgatg tcatgttatg tggcactcat agttgggtat gcgaggagct gcttaatttc      420

ctcgatctaa tccactatgg ctga                                             444


<210>  4
<211>  87
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mitochondrial transit DNA

<400>  4
atgttgtcac tacgtcaatc tataagattt ttcaagccag ccacaagaac tttgtgtagc       60

tctagatatc tgcttcagca aaaaccc                                           87


<210>  5
<211>  869
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Arabidopsis promoter sequence

<400>  5
cagtaactgt ggccaactta gttttgaaac aacactaact ggtcgaagca aaaagaaaaa       60

agagtttcat catatatctg atttgatgga ctgtttggag ttaggaccaa acattatcta      120

caaacaaaga cttttctcct aacttgtgat tccttcttaa accctagggg taatattcta      180

ttttccaagg atctttagtt aaaggcaaat ccgggaaatt attgtaatca tttggggaaa      240

catataaaag atttgagtta gatggaagtg acgattaatc caaacatata tatctctttc      300

ttcttatttc ccaaattaac agacaaaagt agaatattgg cttttaacac caatataaaa      360

acttgttcac acctaaacac ttttgtttac tttagggtaa gtgtaaaaag ccaaccaaat      420

ccacctgcac tgatttgacg tttacaaacg ccgttaagtt tgtcaccgtc taaacaaaaa      480

caaagtagaa gctaacggag ctccgttaat aaattgacga aaagcaaacc aagtttttag      540

ctttggtccc cctcttttac caagtgacaa ttgatttaag cagtgtcttg taattataca      600

accatcgatg tccgttgatt taaacagtgt cttgtaatta aaaaaatcag tttacataaa      660

tggaaaattt atcacttagt tttcatcaac ttctgaactt acctttcatg gattaggcaa      720

tactttccat ttttagtaac tcaagtggac cctttacttc ttcaactcca tctctctctt      780

tctatttcac ttctttcttc tcattatatc tcttgtcctc tccaccaaat ctcttcaaca      840

aaaagattaa acaaagagag aagaatcat                                        869


<210>  6
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mitochonrial transit peptide fused to orf147

<400>  6

Met Leu Ser Leu Arg Gln Ser Ile Arg Phe Phe Lys Pro Ala Thr Arg 
1               5                   10                  15      


Thr Leu Cys Ser Ser Arg Tyr Leu Leu Gln Gln Lys Pro Met His Leu 
            20                  25                  30          


Val Leu Ser Phe Phe Pro Val Cys Arg Ser Ala Ser Lys Glu Arg Lys 
        35                  40                  45              


Leu Lys Ala Asn Lys Asp Lys Met Thr Arg Glu Ile Lys Leu Tyr Val 
    50                  55                  60                  


Asp Thr Thr Pro Ser Asp Leu Asp Phe Met Met Asn Ser Asp Thr Asp 
65                  70                  75                  80  


Leu Gln Ser Leu Ser Phe Pro Asp Ser Ser Asp Ala Gln Ser Ala Ser 
                85                  90                  95      


Pro Asp Leu Asp Leu Leu Trp Asp Arg Val Cys Gly Glu Tyr His Lys 
            100                 105                 110         


Cys Val His Glu Ser Gly Arg Val Leu Pro Pro Glu Trp Thr Met Pro 
        115                 120                 125             


Asp Leu Val Arg Ala Val Ile Ser Asp Asp Glu Ala Ile Glu Gln Gly 
    130                 135                 140                 


Phe Leu Thr Asp Ala Tyr Tyr Asp Val Met Leu Cys Gly Thr His Ser 
145                 150                 155                 160 


Trp Val Cys Glu Glu Leu Leu Asn Phe Leu Asp Leu Ile His Tyr Gly 
                165                 170                 175     


<210>  7
<211>  531
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mitochondrial transit peprtide fused to orf147 DNA

<400>  7
atgttgtcac tacgtcaatc tataagattt ttcaagccag ccacaagaac tttgtgtagc       60

tctagatatc tgcttcagca aaaacccatg catctggttc tatctttttt tccggtatgc      120

cgctccgcca gcaaggagcg aaaactaaaa gcaaacaaag ataagatgac cagagagatc      180

aagctatatg tggataccac ccctagtgat ttggatttta tgatgaatag tgatacggat      240

ttgcagtcct tgtctttccc ggattcgtct gacgcacaga gtgcttcacc ggacttggac      300

ctattatggg atcgagtttg tggtgaatac cacaagtgtg tgcatgaatc cgggagggtc      360

ttacccccgg aatggacgat gcccgacctt gttcgggctg ttatttccga cgatgaagct      420

attgagcagg gctttctgac ggatgcctac tatgatgtca tgttatgtgg cactcatagt      480

tgggtatgcg aggagctgct taatttcctc gatctaatcc actatggctg a               531


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Antisense primer sequence for genome walking

<400>  8
aattcaaagt gaaatttttg                                                   20


<210>  9
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer sequence orf133

<400>  9
atgcagttac tctttgagtt gga                                               23


<210>  10
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer sequence orf133

<400>  10
tcatgctctt aacttacctt ctg                                               23


<210>  11
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer sequence orf147

<400>  11
atgcatctgg ttctatctt                                                    19


<210>  12
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer sequence orf147

<400>  12
tcagccatag tggattagat cgag                                              24


<210>  13
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer sequence with Ndel restriction site for orf133

<400>  13
tcagccatag tggattagat cgag                                              24


<210>  14
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer sequence with SalI restriction site for orf133

<400>  14
atagtcgact catgctctta acttaccttc tg                                     32


<210>  15
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer sequence with Ndel restriction site for orf147

<400>  15
tatcatatgc atctggttct atctt                                             25


<210>  16
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer sequence with SalI restriction site for orf147

<400>  16
tatgtcgact cagccatagt ggattagatc g                                      31


<210>  17
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer with KpnI site for amplifying AtAP3 promoter

<400>  17
taggtaccca gtaactgtgg ccaacttagt t                                      31


<210>  18
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer with NdeI site for amplifying AtAP3 promoter

<400>  18
tcagatcata tgattcttct ctctttgttt aatct                                  35


<210>  19
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for amplifying CoxIV mitochondrial signal peptide

<400>  19
atgttgtcac tacgtcaatc tataag                                            26


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for amplifying CoxIV mitochondrial signal peptide

<400>  20
gggtttttgc tgaagcagat                                                   20


<210>  21
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for amplifying CoxIV orf147 fusion

<400>  21
atctgcttca gcaaaaaccc atgcatctgg ttctatcttt ttttc                       45


<210>  22
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for amplifying CoxIV orf147 fusion

<400>  22
gaaaaaaaga tagaaccaga tgcatgggtt tttgctgaag cagat                       45


<210>  23
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer with NdeI restriction site for amplifying CoxIV 
       orf147 fusion

<400>  23
tatcatatgt tgtcactacg tcaatctata ag                                     32


<210>  24
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer with NotI restriction site for amplifying CoxIV 
       orf147 fusion

<400>  24
gcggccgctc agccatagtg gattagatcg                                        30


<210>  25
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer sequence for amplifying DYT1

<400>  25
gaagctcctc ctgagattga tg                                                22


<210>  26
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer sequence for amplifying DYT1

<400>  26
cttcctctcc ccaatcttac ac                                                22


<210>  27
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer sequence for amplifying AMS

<400>  27
aggctctatg caaaacgaaa ag                                                22


<210>  28
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer sequence for amplifying AMS

<400>  28
ggttgtggta atggttgatg tg                                                22


<210>  29
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer sequence for amplifying SAND

<400>  29
gtgcagacac aaggttgtca gt                                                22


<210>  30
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer sequence for amplifying SAND

<400>  30
ggtaggcaga ttggtgagaa ag                                                22


<210>  31
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer sequence for amplifying TIP41

<400>  31
gaagatgagg caccaactgt tc                                                22


<210>  32
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer sequence for amplifying TIP41

<400>  32
gcttaatcac tggaagcctc tg                                                22


<210>  33
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer sequence for amplifying UNK

<400>  33
gctgagaagc atgttcagga gt                                                22


<210>  34
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer sequence for amplifying UNK

<400>  34
gttcatgagc tcagagagac ca                                                22


<210>  35
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer sequence for amplifying C3H

<400>  35
agttcgacag agtggttgga ct                                                22


<210>  36
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer sequence for amplifying C3H

<400>  36
gcttcggtga ggtagcatta ga                                                22


<210>  37
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer sequence for amplifying CCoAOMT

<400>  37
ctggctatgg atgtcaacag ag                                                22


<210>  38
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer sequence for amplifying CCoAOMT

<400>  38
gttccatggt tcttctcgtc ag                                                22


<210>  39
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer sequence for amplifying 4CL

<400>  39
aggaaccttt tccggttaag tc                                                22


<210>  40
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer sequence for amplifying 4CL

<400>  40
gatctggtga ccacgaatac aa                                                22


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nad7pGER

<400>  41
ctatccacct ctccagacac                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nad7p1R

<400>  42
caaaaatttc acttcgaatt                                                   20


<210>  43
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RACEF1

<400>  43
atgacgacta ggaacgggca aatc                                              24


<210>  44
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RACE R1

<400>  44
gatcgaggaa attaagcagc tc                                                22


<210>  45
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RACE R2

<400>  45
cccgacagag agggaaaag                                                    19


<210>  46
<211>  402
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  orf 133

<400>  46
atgcagttac tctttgagtt ggagtctctt ccggggtttt gctccagcct gtcccttaca       60

gatccagtag ttgcagttcc cccttttctg tcgtcggaaa gaggctctaa caagacctcg      120

aaccagcacc tctgggagaa aaagcgaact gcccttcgtt cttatcgtga caaaagttct      180

gttaggttct tagtagcaat cggcgacctt ttccttcttc tttccctttt ccctctctgt      240

cggggtcagg ggtgtcttcc ctttgctggc catcttggaa gaaatagttt aggaaggtca      300

gggatacgat ttgaatcccg agccccgata gccaatccga ataaagcggc agatctgcgg      360

actcaggaaa gaaagctttc agaaggtaag ttaagagcat ga                         402


