﻿               SEQUENCE LISTING

<110> Friedrich-Alexander-Universität Erlangen-Nürnberg
      Westend-Innovation UG     
	   
<120> A novel CD33-, CD16- and CD123-specific single chain triplebody

<130> Z2341 PCT

<150> EP16 20 2026.7
<151> 2016-12-02

<160> 8

<170> BiSSAP 1.2

<210> 1
<211> 793
<212> PRT
<213> Artificial Sequence

<220> 
<223> SPM-2 with HIS tag (without leader)

<400> 1
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Ile Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Pro Leu Thr Ser Tyr 
            20                  25                  30          
Gly Val Ser Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              
Gly Val Ile Trp Gly Asp Gly Ser Thr Asn Tyr His Ser Ala Leu Ile 
    50                  55                  60                  
Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  
Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      
Arg Asp Thr Tyr Tyr Pro Tyr Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         
Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             
Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
    130                 135                 140                 
Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser 
145                 150                 155                 160 
Gln Asp Val Ser Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
                165                 170                 175     
Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val 
            180                 185                 190         
Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
        195                 200                 205             
Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln 
    210                 215                 220                 
His Tyr Ser Thr Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile 
225                 230                 235                 240 
Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
                245                 250                 255     
Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ser Ser 
            260                 265                 270         
Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser 
        275                 280                 285             
Gln Ser Val Asp Phe Asp Gly Asp Ser Phe Met Asn Trp Tyr Gln Gln 
    290                 295                 300                 
Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Thr Thr Ser Asn Leu 
305                 310                 315                 320 
Glu Ser Gly Val Pro Ser Arg Phe Ser Ala Ser Gly Ser Gly Thr Asp 
                325                 330                 335     
Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
            340                 345                 350         
Tyr Cys Gln Gln Ser Asn Glu Asp Pro Tyr Thr Phe Gly Cys Gly Thr 
        355                 360                 365             
Lys Val Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    370                 375                 380                 
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu 
385                 390                 395                 400 
Ser Gly Gly Gly Asp Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys 
                405                 410                 415     
Ala Phe Ser Gly Phe Ser Leu Arg Thr Ser Gly Met Gly Val Gly Trp 
            420                 425                 430         
Ile Arg Gln Ala Pro Gly Lys Cys Leu Glu Trp Val Ala His Ile Trp 
        435                 440                 445             
Trp Asp Asp Asp Lys Arg Tyr Asn Pro Ser Val Lys Gly Arg Phe Thr 
    450                 455                 460                 
Ile Ser Lys Asp Thr Ser Ser Asn Thr Val Tyr Leu Gln Met Asn Ser 
465                 470                 475                 480 
Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Gln Ile Asn Pro 
                485                 490                 495     
Ala Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
            500                 505                 510         
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
        515                 520                 525             
Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
    530                 535                 540                 
Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr 
545                 550                 555                 560 
Phe Thr Asp Tyr Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Cys 
                565                 570                 575     
Leu Glu Trp Leu Ala Leu Ile Arg Ser Lys Ala Asp Gly Tyr Thr Thr 
            580                 585                 590         
Glu Tyr Ser Ala Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp 
        595                 600                 605             
Ser Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp 
    610                 615                 620                 
Thr Ala Val Tyr Tyr Cys Ala Arg Asp Ala Ala Tyr Tyr Ser Tyr Tyr 
625                 630                 635                 640 
Ser Pro Glu Gly Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr 
                645                 650                 655     
Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
            660                 665                 670         
Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
        675                 680                 685             
Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Val Asp 
    690                 695                 700                 
Ser Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Ala 
705                 710                 715                 720 
Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Ser Gly Val Pro Ser Arg Phe 
                725                 730                 735     
Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
            740                 745                 750         
Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Ser Thr 
        755                 760                 765             
Pro Trp Thr Phe Gly Cys Gly Thr Lys Val Glu Ile Lys Arg Gly Gly 
    770                 775                 780                 
Gly Gly Ser His His His His His His 
785                 790             

<210> 2
<211> 2382
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..2382
<223> /mol_type="unassigned DNA"
      /note="SPM-2 with HIS tag (without leader)"
      /organism="Artificial Sequence"

<400> 2
gaagtccaac tcgttgagag cggcggaggg cttattcagc ctggggggtc attgcgcctc      60

tcatgtgccg cctctggttt ccctctgacg agctacggcg tgtcctgggt gaggcagcct     120

ccaggaaaag gtcttgaatg gttgggcgtg atctggggcg atggcagcac taactatcac     180

tccgcactga taagtagatt caccatttcc cgggacaatt ccaagaacac gctctatctt     240

cagatgaatt ccctgagagc cgaggacaca gcagtttact attgtgctcg ggatacctac     300

tatccctact acgccatgga ttactggggc caagggacaa ctgttaccgt gagctccggc     360

ggtggaggaa gtggtggagg tggcagtggc ggcggtgggt ccgacattca aatgactcag     420

agcccttcta gcctctctgc cagcgtgggc gaccgggtga ccatcacatg caaggccagt     480

caggatgtgt ccaccgccgt ggcttggtat cagcaaaagc ctggcaaggc tcccaagctg     540

ctcatctact ctgccagtta tcgatatacc ggggttccct cacgtttcag cggctctggg     600

tcagggactg actttactct gaccatttct agcctccaac cagaggattt tgcaacttac     660

tattgtcagc agcactactc taccccactc acatttggcc agggaaccaa attggagatc     720

aagcggggcg gtggtgggtc cggcggcgga ggcagtggcg ggggaggtag cggtggcggc     780

gggagtgata tagtcttgac ccagtctcca tcctccctgt ccgcttcagt gggcgacaga     840

gtcacaatca cgtgcaaggc cagccagtct gtcgactttg acggcgactc ctttatgaat     900

tggtaccaac agaagccagg gaaagctccc aagttgctga tctataccac ttccaatctg     960

gaaagcggtg ttccctcacg cttctcagca tcaggaagcg ggacagactt tacgctgacc    1020

attagctcac tgcagccaga ggatttcgct acatactact gccagcaatc aaacgaggat    1080

ccttatacct ttggctgcgg aacaaaggtc gaaatcaaga gaggcggagg tgggtcaggt    1140

ggaggcggct ctggcggagg aggtagcgga ggaggcggat ccgaggttca gctggtggaa    1200

tccggtggcg gagatgtgca gcccggagga tctctgaggc tcagttgtgc tttctccggc    1260

ttcagtctgc gtacttcagg catgggcgtg gggtggatta ggcaggcacc tggtaagtgc    1320

cttgagtggg tcgcccacat ttggtgggat gacgacaaac ggtacaatcc ctcagtcaag    1380

ggtaggttta ccatttccaa ggacacatcc agcaacacag tatatctgca gatgaacagc    1440

ttgagagccg aggatacagc tgtctactac tgcgctcaga tcaaccccgc ttggttcgcc    1500

tattggggcc aggggaccct ggtgacagtg agttctggtg gaggtggctc aggggggggc    1560

ggttctggcg gtggggggtc cggggggggt ggtagtgaag tccagctggt ggagagtgga    1620

ggagggctgg tgcaacccgg agggtcactt aggctgagtt gcgctgcatc tggcttcaca    1680

ttcactgact actacatgtc ctgggtgcgc caggcacccg gtaagtgcct ggaatggctt    1740

gctctgattc gttctaaagc cgatggatac actaccgaat atagtgcaag cgtcaaagga    1800

aggttcacaa tctcccgaga tgactccaag aatagtctgt atctgcaaat gaactccctc    1860

aaaacagagg acaccgctgt atattactgt gcccgcgacg ccgcttacta cagttattac    1920

tctccagaag gagccatgga ttattggggt caggggacct ccgttaccgt atctagtggc    1980

ggaggcggtt ctggaggagg tggatccggg ggaggcggca gcgacatcca gatgacacag    2040

tcccctagct ccctgtctgc atccgtggga gatcgagtga ctatcacttg caaagcaagt    2100

cagaacgtgg atagcgccgt tgcatggtat cagcagaagc ccggaaaggc cccaaaagcc    2160

ttgatctact ccgcctccta ccggtattct ggggtaccat cacgcttctc tgggtctggc    2220

agcggaaccg actttaccct gactataagc agcctgcagc ccgaggactt tgctacctat    2280

tactgtcagc agtattacag cactccttgg acttttggct gtgggacgaa agtggagatc    2340

aaacgagggg gcggtggctc tcaccatcat caccatcatt ga                       2382


<210> 3
<211> 782
<212> PRT
<213> Artificial Sequence

<220> 
<223> SPM-2 without HIS tag (without leader)

<400> 3
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Ile Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Pro Leu Thr Ser Tyr 
            20                  25                  30          
Gly Val Ser Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              
Gly Val Ile Trp Gly Asp Gly Ser Thr Asn Tyr His Ser Ala Leu Ile 
    50                  55                  60                  
Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  
Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      
Arg Asp Thr Tyr Tyr Pro Tyr Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         
Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             
Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
    130                 135                 140                 
Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser 
145                 150                 155                 160 
Gln Asp Val Ser Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
                165                 170                 175     
Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val 
            180                 185                 190         
Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
        195                 200                 205             
Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln 
    210                 215                 220                 
His Tyr Ser Thr Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile 
225                 230                 235                 240 
Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
                245                 250                 255     
Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ser Ser 
            260                 265                 270         
Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser 
        275                 280                 285             
Gln Ser Val Asp Phe Asp Gly Asp Ser Phe Met Asn Trp Tyr Gln Gln 
    290                 295                 300                 
Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Thr Thr Ser Asn Leu 
305                 310                 315                 320 
Glu Ser Gly Val Pro Ser Arg Phe Ser Ala Ser Gly Ser Gly Thr Asp 
                325                 330                 335     
Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
            340                 345                 350         
Tyr Cys Gln Gln Ser Asn Glu Asp Pro Tyr Thr Phe Gly Cys Gly Thr 
        355                 360                 365             
Lys Val Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    370                 375                 380                 
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu 
385                 390                 395                 400 
Ser Gly Gly Gly Asp Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys 
                405                 410                 415     
Ala Phe Ser Gly Phe Ser Leu Arg Thr Ser Gly Met Gly Val Gly Trp 
            420                 425                 430         
Ile Arg Gln Ala Pro Gly Lys Cys Leu Glu Trp Val Ala His Ile Trp 
        435                 440                 445             
Trp Asp Asp Asp Lys Arg Tyr Asn Pro Ser Val Lys Gly Arg Phe Thr 
    450                 455                 460                 
Ile Ser Lys Asp Thr Ser Ser Asn Thr Val Tyr Leu Gln Met Asn Ser 
465                 470                 475                 480 
Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Gln Ile Asn Pro 
                485                 490                 495     
Ala Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
            500                 505                 510         
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
        515                 520                 525             
Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
    530                 535                 540                 
Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr 
545                 550                 555                 560 
Phe Thr Asp Tyr Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Cys 
                565                 570                 575     
Leu Glu Trp Leu Ala Leu Ile Arg Ser Lys Ala Asp Gly Tyr Thr Thr 
            580                 585                 590         
Glu Tyr Ser Ala Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp 
        595                 600                 605             
Ser Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp 
    610                 615                 620                 
Thr Ala Val Tyr Tyr Cys Ala Arg Asp Ala Ala Tyr Tyr Ser Tyr Tyr 
625                 630                 635                 640 
Ser Pro Glu Gly Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr 
                645                 650                 655     
Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
            660                 665                 670         
Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
        675                 680                 685             
Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Val Asp 
    690                 695                 700                 
Ser Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Ala 
705                 710                 715                 720 
Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Ser Gly Val Pro Ser Arg Phe 
                725                 730                 735     
Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
            740                 745                 750         
Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Ser Thr 
        755                 760                 765             
Pro Trp Thr Phe Gly Cys Gly Thr Lys Val Glu Ile Lys Arg 
    770                 775                 780         

<210> 4
<211> 2349
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..2349
<223> /mol_type="unassigned DNA"
      /note="SPM-2 without HIS tag (without leader)"
      /organism="Artificial Sequence"

<400> 4
gaagtccaac tcgttgagag cggcggaggg cttattcagc ctggggggtc attgcgcctc      60

tcatgtgccg cctctggttt ccctctgacg agctacggcg tgtcctgggt gaggcagcct     120

ccaggaaaag gtcttgaatg gttgggcgtg atctggggcg atggcagcac taactatcac     180

tccgcactga taagtagatt caccatttcc cgggacaatt ccaagaacac gctctatctt     240

cagatgaatt ccctgagagc cgaggacaca gcagtttact attgtgctcg ggatacctac     300

tatccctact acgccatgga ttactggggc caagggacaa ctgttaccgt gagctccggc     360

ggtggaggaa gtggtggagg tggcagtggc ggcggtgggt ccgacattca aatgactcag     420

agcccttcta gcctctctgc cagcgtgggc gaccgggtga ccatcacatg caaggccagt     480

caggatgtgt ccaccgccgt ggcttggtat cagcaaaagc ctggcaaggc tcccaagctg     540

ctcatctact ctgccagtta tcgatatacc ggggttccct cacgtttcag cggctctggg     600

tcagggactg actttactct gaccatttct agcctccaac cagaggattt tgcaacttac     660

tattgtcagc agcactactc taccccactc acatttggcc agggaaccaa attggagatc     720

aagcggggcg gtggtgggtc cggcggcgga ggcagtggcg ggggaggtag cggtggcggc     780

gggagtgata tagtcttgac ccagtctcca tcctccctgt ccgcttcagt gggcgacaga     840

gtcacaatca cgtgcaaggc cagccagtct gtcgactttg acggcgactc ctttatgaat     900

tggtaccaac agaagccagg gaaagctccc aagttgctga tctataccac ttccaatctg     960

gaaagcggtg ttccctcacg cttctcagca tcaggaagcg ggacagactt tacgctgacc    1020

attagctcac tgcagccaga ggatttcgct acatactact gccagcaatc aaacgaggat    1080

ccttatacct ttggctgcgg aacaaaggtc gaaatcaaga gaggcggagg tgggtcaggt    1140

ggaggcggct ctggcggagg aggtagcgga ggaggcggat ccgaggttca gctggtggaa    1200

tccggtggcg gagatgtgca gcccggagga tctctgaggc tcagttgtgc tttctccggc    1260

ttcagtctgc gtacttcagg catgggcgtg gggtggatta ggcaggcacc tggtaagtgc    1320

cttgagtggg tcgcccacat ttggtgggat gacgacaaac ggtacaatcc ctcagtcaag    1380

ggtaggttta ccatttccaa ggacacatcc agcaacacag tatatctgca gatgaacagc    1440

ttgagagccg aggatacagc tgtctactac tgcgctcaga tcaaccccgc ttggttcgcc    1500

tattggggcc aggggaccct ggtgacagtg agttctggtg gaggtggctc aggggggggc    1560

ggttctggcg gtggggggtc cggggggggt ggtagtgaag tccagctggt ggagagtgga    1620

ggagggctgg tgcaacccgg agggtcactt aggctgagtt gcgctgcatc tggcttcaca    1680

ttcactgact actacatgtc ctgggtgcgc caggcacccg gtaagtgcct ggaatggctt    1740

gctctgattc gttctaaagc cgatggatac actaccgaat atagtgcaag cgtcaaagga    1800

aggttcacaa tctcccgaga tgactccaag aatagtctgt atctgcaaat gaactccctc    1860

aaaacagagg acaccgctgt atattactgt gcccgcgacg ccgcttacta cagttattac    1920

tctccagaag gagccatgga ttattggggt caggggacct ccgttaccgt atctagtggc    1980

ggaggcggtt ctggaggagg tggatccggg ggaggcggca gcgacatcca gatgacacag    2040

tcccctagct ccctgtctgc atccgtggga gatcgagtga ctatcacttg caaagcaagt    2100

cagaacgtgg atagcgccgt tgcatggtat cagcagaagc ccggaaaggc cccaaaagcc    2160

ttgatctact ccgcctccta ccggtattct ggggtaccat cacgcttctc tgggtctggc    2220

agcggaaccg actttaccct gactataagc agcctgcagc ccgaggactt tgctacctat    2280

tactgtcagc agtattacag cactccttgg acttttggct gtgggacgaa agtggagatc    2340

aaacgatga                                                            2349


<210> 5
<211> 813
<212> PRT
<213> Artificial Sequence

<220> 
<223> SPM-2 with leader sequence and HIS tag

<400> 5
Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      
Gly Ser Thr Gly Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Ile 
            20                  25                  30          
Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Pro 
        35                  40                  45              
Leu Thr Ser Tyr Gly Val Ser Trp Val Arg Gln Pro Pro Gly Lys Gly 
    50                  55                  60                  
Leu Glu Trp Leu Gly Val Ile Trp Gly Asp Gly Ser Thr Asn Tyr His 
65                  70                  75                  80  
Ser Ala Leu Ile Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
                85                  90                  95      
Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            100                 105                 110         
Tyr Tyr Cys Ala Arg Asp Thr Tyr Tyr Pro Tyr Tyr Ala Met Asp Tyr 
        115                 120                 125             
Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln 
145                 150                 155                 160 
Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
                165                 170                 175     
Cys Lys Ala Ser Gln Asp Val Ser Thr Ala Val Ala Trp Tyr Gln Gln 
            180                 185                 190         
Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Tyr Arg 
        195                 200                 205             
Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 
Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 
Tyr Cys Gln Gln His Tyr Ser Thr Pro Leu Thr Phe Gly Gln Gly Thr 
                245                 250                 255     
Lys Leu Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            260                 265                 270         
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln 
        275                 280                 285             
Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
    290                 295                 300                 
Cys Lys Ala Ser Gln Ser Val Asp Phe Asp Gly Asp Ser Phe Met Asn 
305                 310                 315                 320 
Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Thr 
                325                 330                 335     
Thr Ser Asn Leu Glu Ser Gly Val Pro Ser Arg Phe Ser Ala Ser Gly 
            340                 345                 350         
Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp 
        355                 360                 365             
Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Asn Glu Asp Pro Tyr Thr Phe 
    370                 375                 380                 
Gly Cys Gly Thr Lys Val Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly 
385                 390                 395                 400 
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val 
                405                 410                 415     
Gln Leu Val Glu Ser Gly Gly Gly Asp Val Gln Pro Gly Gly Ser Leu 
            420                 425                 430         
Arg Leu Ser Cys Ala Phe Ser Gly Phe Ser Leu Arg Thr Ser Gly Met 
        435                 440                 445             
Gly Val Gly Trp Ile Arg Gln Ala Pro Gly Lys Cys Leu Glu Trp Val 
    450                 455                 460                 
Ala His Ile Trp Trp Asp Asp Asp Lys Arg Tyr Asn Pro Ser Val Lys 
465                 470                 475                 480 
Gly Arg Phe Thr Ile Ser Lys Asp Thr Ser Ser Asn Thr Val Tyr Leu 
                485                 490                 495     
Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
            500                 505                 510         
Gln Ile Asn Pro Ala Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val 
        515                 520                 525             
Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    530                 535                 540                 
Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly 
545                 550                 555                 560 
Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala 
                565                 570                 575     
Ser Gly Phe Thr Phe Thr Asp Tyr Tyr Met Ser Trp Val Arg Gln Ala 
            580                 585                 590         
Pro Gly Lys Cys Leu Glu Trp Leu Ala Leu Ile Arg Ser Lys Ala Asp 
        595                 600                 605             
Gly Tyr Thr Thr Glu Tyr Ser Ala Ser Val Lys Gly Arg Phe Thr Ile 
    610                 615                 620                 
Ser Arg Asp Asp Ser Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu 
625                 630                 635                 640 
Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Ala Ala Tyr 
                645                 650                 655     
Tyr Ser Tyr Tyr Ser Pro Glu Gly Ala Met Asp Tyr Trp Gly Gln Gly 
            660                 665                 670         
Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        675                 680                 685             
Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
    690                 695                 700                 
Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser 
705                 710                 715                 720 
Gln Asn Val Asp Ser Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
                725                 730                 735     
Ala Pro Lys Ala Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Ser Gly Val 
            740                 745                 750         
Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
        755                 760                 765             
Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln 
    770                 775                 780                 
Tyr Tyr Ser Thr Pro Trp Thr Phe Gly Cys Gly Thr Lys Val Glu Ile 
785                 790                 795                 800 
Lys Arg Gly Gly Gly Gly Ser His His His His His His 
                805                 810             

<210> 6
<211> 2442
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..2442
<223> /mol_type="unassigned DNA"
      /note="SPM-2 with leader sequence and HIS tag"
      /organism="Artificial Sequence"

<400> 6
atggagactg atacattgct gctctgggta ctgcttctgt gggtccctgg ttctaccggc      60

gaagtccaac tcgttgagag cggcggaggg cttattcagc ctggggggtc attgcgcctc     120

tcatgtgccg cctctggttt ccctctgacg agctacggcg tgtcctgggt gaggcagcct     180

ccaggaaaag gtcttgaatg gttgggcgtg atctggggcg atggcagcac taactatcac     240

tccgcactga taagtagatt caccatttcc cgggacaatt ccaagaacac gctctatctt     300

cagatgaatt ccctgagagc cgaggacaca gcagtttact attgtgctcg ggatacctac     360

tatccctact acgccatgga ttactggggc caagggacaa ctgttaccgt gagctccggc     420

ggtggaggaa gtggtggagg tggcagtggc ggcggtgggt ccgacattca aatgactcag     480

agcccttcta gcctctctgc cagcgtgggc gaccgggtga ccatcacatg caaggccagt     540

caggatgtgt ccaccgccgt ggcttggtat cagcaaaagc ctggcaaggc tcccaagctg     600

ctcatctact ctgccagtta tcgatatacc ggggttccct cacgtttcag cggctctggg     660

tcagggactg actttactct gaccatttct agcctccaac cagaggattt tgcaacttac     720

tattgtcagc agcactactc taccccactc acatttggcc agggaaccaa attggagatc     780

aagcggggcg gtggtgggtc cggcggcgga ggcagtggcg ggggaggtag cggtggcggc     840

gggagtgata tagtcttgac ccagtctcca tcctccctgt ccgcttcagt gggcgacaga     900

gtcacaatca cgtgcaaggc cagccagtct gtcgactttg acggcgactc ctttatgaat     960

tggtaccaac agaagccagg gaaagctccc aagttgctga tctataccac ttccaatctg    1020

gaaagcggtg ttccctcacg cttctcagca tcaggaagcg ggacagactt tacgctgacc    1080

attagctcac tgcagccaga ggatttcgct acatactact gccagcaatc aaacgaggat    1140

ccttatacct ttggctgcgg aacaaaggtc gaaatcaaga gaggcggagg tgggtcaggt    1200

ggaggcggct ctggcggagg aggtagcgga ggaggcggat ccgaggttca gctggtggaa    1260

tccggtggcg gagatgtgca gcccggagga tctctgaggc tcagttgtgc tttctccggc    1320

ttcagtctgc gtacttcagg catgggcgtg gggtggatta ggcaggcacc tggtaagtgc    1380

cttgagtggg tcgcccacat ttggtgggat gacgacaaac ggtacaatcc ctcagtcaag    1440

ggtaggttta ccatttccaa ggacacatcc agcaacacag tatatctgca gatgaacagc    1500

ttgagagccg aggatacagc tgtctactac tgcgctcaga tcaaccccgc ttggttcgcc    1560

tattggggcc aggggaccct ggtgacagtg agttctggtg gaggtggctc aggggggggc    1620

ggttctggcg gtggggggtc cggggggggt ggtagtgaag tccagctggt ggagagtgga    1680

ggagggctgg tgcaacccgg agggtcactt aggctgagtt gcgctgcatc tggcttcaca    1740

ttcactgact actacatgtc ctgggtgcgc caggcacccg gtaagtgcct ggaatggctt    1800

gctctgattc gttctaaagc cgatggatac actaccgaat atagtgcaag cgtcaaagga    1860

aggttcacaa tctcccgaga tgactccaag aatagtctgt atctgcaaat gaactccctc    1920

aaaacagagg acaccgctgt atattactgt gcccgcgacg ccgcttacta cagttattac    1980

tctccagaag gagccatgga ttattggggt caggggacct ccgttaccgt atctagtggc    2040

ggaggcggtt ctggaggagg tggatccggg ggaggcggca gcgacatcca gatgacacag    2100

tcccctagct ccctgtctgc atccgtggga gatcgagtga ctatcacttg caaagcaagt    2160

cagaacgtgg atagcgccgt tgcatggtat cagcagaagc ccggaaaggc cccaaaagcc    2220

ttgatctact ccgcctccta ccggtattct ggggtaccat cacgcttctc tgggtctggc    2280

agcggaaccg actttaccct gactataagc agcctgcagc ccgaggactt tgctacctat    2340

tactgtcagc agtattacag cactccttgg acttttggct gtgggacgaa agtggagatc    2400

aaacgagggg gcggtggctc tcaccatcat caccatcatt ga                       2442


<210> 7
<211> 802
<212> PRT
<213> Artificial Sequence

<220> 
<223> SPM-2 with leader sequence and without HIS tag

<400> 7
Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      
Gly Ser Thr Gly Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Ile 
            20                  25                  30          
Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Pro 
        35                  40                  45              
Leu Thr Ser Tyr Gly Val Ser Trp Val Arg Gln Pro Pro Gly Lys Gly 
    50                  55                  60                  
Leu Glu Trp Leu Gly Val Ile Trp Gly Asp Gly Ser Thr Asn Tyr His 
65                  70                  75                  80  
Ser Ala Leu Ile Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
                85                  90                  95      
Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            100                 105                 110         
Tyr Tyr Cys Ala Arg Asp Thr Tyr Tyr Pro Tyr Tyr Ala Met Asp Tyr 
        115                 120                 125             
Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln 
145                 150                 155                 160 
Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
                165                 170                 175     
Cys Lys Ala Ser Gln Asp Val Ser Thr Ala Val Ala Trp Tyr Gln Gln 
            180                 185                 190         
Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Tyr Arg 
        195                 200                 205             
Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 
Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 
Tyr Cys Gln Gln His Tyr Ser Thr Pro Leu Thr Phe Gly Gln Gly Thr 
                245                 250                 255     
Lys Leu Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            260                 265                 270         
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln 
        275                 280                 285             
Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
    290                 295                 300                 
Cys Lys Ala Ser Gln Ser Val Asp Phe Asp Gly Asp Ser Phe Met Asn 
305                 310                 315                 320 
Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Thr 
                325                 330                 335     
Thr Ser Asn Leu Glu Ser Gly Val Pro Ser Arg Phe Ser Ala Ser Gly 
            340                 345                 350         
Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp 
        355                 360                 365             
Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Asn Glu Asp Pro Tyr Thr Phe 
    370                 375                 380                 
Gly Cys Gly Thr Lys Val Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly 
385                 390                 395                 400 
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val 
                405                 410                 415     
Gln Leu Val Glu Ser Gly Gly Gly Asp Val Gln Pro Gly Gly Ser Leu 
            420                 425                 430         
Arg Leu Ser Cys Ala Phe Ser Gly Phe Ser Leu Arg Thr Ser Gly Met 
        435                 440                 445             
Gly Val Gly Trp Ile Arg Gln Ala Pro Gly Lys Cys Leu Glu Trp Val 
    450                 455                 460                 
Ala His Ile Trp Trp Asp Asp Asp Lys Arg Tyr Asn Pro Ser Val Lys 
465                 470                 475                 480 
Gly Arg Phe Thr Ile Ser Lys Asp Thr Ser Ser Asn Thr Val Tyr Leu 
                485                 490                 495     
Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
            500                 505                 510         
Gln Ile Asn Pro Ala Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val 
        515                 520                 525             
Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    530                 535                 540                 
Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly 
545                 550                 555                 560 
Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala 
                565                 570                 575     
Ser Gly Phe Thr Phe Thr Asp Tyr Tyr Met Ser Trp Val Arg Gln Ala 
            580                 585                 590         
Pro Gly Lys Cys Leu Glu Trp Leu Ala Leu Ile Arg Ser Lys Ala Asp 
        595                 600                 605             
Gly Tyr Thr Thr Glu Tyr Ser Ala Ser Val Lys Gly Arg Phe Thr Ile 
    610                 615                 620                 
Ser Arg Asp Asp Ser Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu 
625                 630                 635                 640 
Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Ala Ala Tyr 
                645                 650                 655     
Tyr Ser Tyr Tyr Ser Pro Glu Gly Ala Met Asp Tyr Trp Gly Gln Gly 
            660                 665                 670         
Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        675                 680                 685             
Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
    690                 695                 700                 
Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser 
705                 710                 715                 720 
Gln Asn Val Asp Ser Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
                725                 730                 735     
Ala Pro Lys Ala Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Ser Gly Val 
            740                 745                 750         
Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
        755                 760                 765             
Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln 
    770                 775                 780                 
Tyr Tyr Ser Thr Pro Trp Thr Phe Gly Cys Gly Thr Lys Val Glu Ile 
785                 790                 795                 800 
Lys Arg 
        

<210> 8
<211> 2409
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..2409
<223> /mol_type="unassigned DNA"
      /note="SPM-2 with leader sequence and without HIS tag"
      /organism="Artificial Sequence"

<400> 8
atggagactg atacattgct gctctgggta ctgcttctgt gggtccctgg ttctaccggc      60

gaagtccaac tcgttgagag cggcggaggg cttattcagc ctggggggtc attgcgcctc     120

tcatgtgccg cctctggttt ccctctgacg agctacggcg tgtcctgggt gaggcagcct     180

ccaggaaaag gtcttgaatg gttgggcgtg atctggggcg atggcagcac taactatcac     240

tccgcactga taagtagatt caccatttcc cgggacaatt ccaagaacac gctctatctt     300

cagatgaatt ccctgagagc cgaggacaca gcagtttact attgtgctcg ggatacctac     360

tatccctact acgccatgga ttactggggc caagggacaa ctgttaccgt gagctccggc     420

ggtggaggaa gtggtggagg tggcagtggc ggcggtgggt ccgacattca aatgactcag     480

agcccttcta gcctctctgc cagcgtgggc gaccgggtga ccatcacatg caaggccagt     540

caggatgtgt ccaccgccgt ggcttggtat cagcaaaagc ctggcaaggc tcccaagctg     600

ctcatctact ctgccagtta tcgatatacc ggggttccct cacgtttcag cggctctggg     660

tcagggactg actttactct gaccatttct agcctccaac cagaggattt tgcaacttac     720

tattgtcagc agcactactc taccccactc acatttggcc agggaaccaa attggagatc     780

aagcggggcg gtggtgggtc cggcggcgga ggcagtggcg ggggaggtag cggtggcggc     840

gggagtgata tagtcttgac ccagtctcca tcctccctgt ccgcttcagt gggcgacaga     900

gtcacaatca cgtgcaaggc cagccagtct gtcgactttg acggcgactc ctttatgaat     960

tggtaccaac agaagccagg gaaagctccc aagttgctga tctataccac ttccaatctg    1020

gaaagcggtg ttccctcacg cttctcagca tcaggaagcg ggacagactt tacgctgacc    1080

attagctcac tgcagccaga ggatttcgct acatactact gccagcaatc aaacgaggat    1140

ccttatacct ttggctgcgg aacaaaggtc gaaatcaaga gaggcggagg tgggtcaggt    1200

ggaggcggct ctggcggagg aggtagcgga ggaggcggat ccgaggttca gctggtggaa    1260

tccggtggcg gagatgtgca gcccggagga tctctgaggc tcagttgtgc tttctccggc    1320

ttcagtctgc gtacttcagg catgggcgtg gggtggatta ggcaggcacc tggtaagtgc    1380

cttgagtggg tcgcccacat ttggtgggat gacgacaaac ggtacaatcc ctcagtcaag    1440

ggtaggttta ccatttccaa ggacacatcc agcaacacag tatatctgca gatgaacagc    1500

ttgagagccg aggatacagc tgtctactac tgcgctcaga tcaaccccgc ttggttcgcc    1560

tattggggcc aggggaccct ggtgacagtg agttctggtg gaggtggctc aggggggggc    1620

ggttctggcg gtggggggtc cggggggggt ggtagtgaag tccagctggt ggagagtgga    1680

ggagggctgg tgcaacccgg agggtcactt aggctgagtt gcgctgcatc tggcttcaca    1740

ttcactgact actacatgtc ctgggtgcgc caggcacccg gtaagtgcct ggaatggctt    1800

gctctgattc gttctaaagc cgatggatac actaccgaat atagtgcaag cgtcaaagga    1860

aggttcacaa tctcccgaga tgactccaag aatagtctgt atctgcaaat gaactccctc    1920

aaaacagagg acaccgctgt atattactgt gcccgcgacg ccgcttacta cagttattac    1980

tctccagaag gagccatgga ttattggggt caggggacct ccgttaccgt atctagtggc    2040

ggaggcggtt ctggaggagg tggatccggg ggaggcggca gcgacatcca gatgacacag    2100

tcccctagct ccctgtctgc atccgtggga gatcgagtga ctatcacttg caaagcaagt    2160

cagaacgtgg atagcgccgt tgcatggtat cagcagaagc ccggaaaggc cccaaaagcc    2220

ttgatctact ccgcctccta ccggtattct ggggtaccat cacgcttctc tgggtctggc    2280

agcggaaccg actttaccct gactataagc agcctgcagc ccgaggactt tgctacctat    2340

tactgtcagc agtattacag cactccttgg acttttggct gtgggacgaa agtggagatc    2400

aaacgatga                                                            2409


