                         SEQUENCE LISTING

<110>  Horizon Discovery Ltd
 
<120>  METHODS FOR CONDITIONAL GENE KNOCK-OUT

<130>  P8715WO1

<150>  GB1620091.7
<151>  2016-11-28

<150>  GB1717695.9
<151>  2017-10-27

<160>  44    

<170>  PatentIn version 3.5

<210>  1
<211>  133
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chimeric intron sequence

<400>  1
gtaagtatca aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga       60

cagagaagac tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc      120

tttctctcca cag                                                         133


<210>  2
<211>  201
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Version 1: Artificial intron with loxP sites

<400>  2
gtaagtatca aggttagaag acaggtttaa ggagaccaat agaaactggg cttataactt       60

cgtatagcat acattatacg aagttatgtc gagacagaga agactcttgc gtttctgata      120

ggcacataac ttcgtatagc atacattata cgaagttatc tattggtctt actgacatcc      180

actttgcctt tctctccaca g                                                201


<210>  3
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Version 1: Expected sequence after Cre treatment

<400>  3
gtaagtatca aggttagaag acaggtttaa ggagaccaat agaaactggg cttataactt       60

cgtatagcat acattatacg aagttatcta ttggtcttac tgacatccac tttgcctttc      120

tctccacag                                                              129


<210>  4
<211>  201
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Version 2: Synthetic intron with loxP sites

<400>  4
gtaagtaata acttcgtata gcatacatta tacgaagtta ttcaaggtta gaagacaggt       60

ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata      120

ggcacataac ttcgtatagc atacattata cgaagttatc tattggtctt actgacatcc      180

actttgcctt tctctccaca g                                                201


<210>  5
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Version 2: Expected sequence after Cre treatment

<400>  5
gtaagtaata acttcgtata gcatacatta tacgaagtta tctattggtc ttactgacat       60

ccactttgcc tttctctcca cag                                               83


<210>  6
<211>  201
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Version 3: Synthetic intron with loxP sites

<400>  6
gtaagtaata acttcgtata gcatacatta tacgaagtta ttcaaggtta gaagacaggt       60

ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata      120

ggcacctatt ggtcttactg acatccactt tgcctttctc tccaataact tcgtatagca      180

tacattatac gaagttatca g                                                201


<210>  7
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Version 3: Expected sequence after Cre treatment

<400>  7
gtaagtaata acttcgtata gcatacatta tacgaagtta tcag                        44


<210>  8
<211>  201
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Version 4: Synthetic intron with loxP sites

<400>  8
gtaagtaata acttcgtata gcatacatta tacgaagtta ttcaaggtta gaagacaggt       60

ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata      120

ggcacctatt ggtcttactg acatccactt tgccataact tcgtatagca tacattatac      180

gaagttattt tctctccaca g                                                201


<210>  9
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Version 4: Expected sequence after Cre-treatment

<400>  9
gtaagtaata acttcgtata gcatacatta tacgaagtta ttttctctcc acag             54


<210>  10
<211>  201
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Version 5: Synthetic intron with loxP sites

<400>  10
gtaagtaata acttcgtata gcatacatta tacgaagtta ttcaaggtta gaagacaggt       60

ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata      120

ggcacctatt ggtcttactg acaataactt cgtatagcat acattatacg aagttattcc      180

actttgcctt tctctccaca g                                                201


<210>  11
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Version 5: Expected sequence after Cre treatment

<400>  11
gtaagtaata acttcgtata gcatacatta tacgaagtta ttccactttg cctttctctc       60

cacag                                                                   65


<210>  12
<211>  516
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NanoLuc sequence with intron insertion sites marked

<400>  12
atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg       60

gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta      120

actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga tatccatgtc      180

atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag      240

gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta      300

atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc      360

gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc      420

gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg      480

accggctggc ggctgtgcga acgcattctg gcgtaa                                516


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CDK4 guide RNA coding strand

<400>  13
ctgatgcgcc agtttctaag                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  METTL16 guide RNA coding strand

<400>  14
tctgacgtgt actctcctaa                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CDK4_genotyping_primer_fwd

<400>  15
tgagaggtgg attgggacct                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CDK4_genotyping _primer _rev

<400>  16
ccagcttgac tgttccacca                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  METTL16_genotyping _primer _fwd

<400>  17
ttctgcctgt ttgccgtaga                                                   20


<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  METTL16_genotyping _primer _rev

<400>  18
ttgtcagaat cctggtgacc g                                                 21


<210>  19
<211>  861
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CDK4

<400>  19
aggtggggtg tgatgatctg tagagaagtg gggaccctga ggaaataatg agaggccatg       60

ttgggttaaa ggggattgaa aagtgagcat ttactctggt caggctgatg gacgtctgtg      120

ccacatcccg aactgaccgg gagatcaagg taaccctggt gtttgagcat gtagaccagg      180

acctaaggac atatctggac aaggcacccc caccaggctt gccagccgaa acgatcaagg      240

tgagtggggt tggtaggcat tgagaggtgg attgggacct ttgtagtaga accttctggg      300

atttcaggta tggtgcctag tttccagtgc atctgtacct cccctttgaa actaggatct      360

gatgcgccag tttctaagag gcctagattt ccttcatgcc aattgcatcg ttcaccgaga      420

tctgaagcca gagaacattc tggtgacaag tggtggaaca gtcaagctgg ctgactttgg      480

cctggccaga atctacagct accagatggc acttacaccc gtggtcagta gaaagatggt      540

accaaaatgg gttctggttg ggagtaggag agtgattgcc cgtagcaatt gagaagtcat      600

gtgcttcatg tgttcagtca agcaagttgt gtttcatggt aacccatggg gtccccatcc      660

attcttccta ttccctttag gttgttacac tctggtaccg agctcccgaa gttcttctgc      720

agtccacata tgcaacacct gtggacatgt ggagtgttgg ctgtatcttt gcagagatgt      780

ttcgtcgaaa gtatgggacc cacataccct ggactacctt gaattcccca aatcgcttgt      840

tcataaacca catccatacc t                                                861


<210>  20
<211>  800
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  METTL16

<400>  20
acttgttcaa agtcacacag tgaggagacg acgagacagg aatttgagcc gagtcattta       60

atctctgtgc tccatatacg gatatgcagt gtttttggtg gaggattgta ctgattttca      120

taagctgtga ctgtgtgaaa cactcgtgta tgtgccactg ggatgagtgc tctgtgtaac      180

tgtgtgtgat ctccctggga ggttcggatg ggtatttggt tctgcctgtt tgccgtagag      240

cttgtacaac atagtgaatc ttactaatca gttttacttc cttttcttta ttcctcagcc      300

ttaattttaa agaccccgaa gcagtcagag ctctgacgtg tactctccta agggaagatt      360

ttggactttc tattgatatt ccattggaga gactaattcc cacagttccc ttgagactca      420

actatattca ctgggtagaa gatctgatcg gtcaccagga ttctgacaaa agtactctcc      480

gaagaggaat tgacataggt atatcatttt aaattctttt ttggctaaac agttttcata      540

agttttgcga gatcaaattc tttgtaaact tttctactgg gtatttgttg ttgtacaaaa      600

gaagctggct acagatacag gttgagcatc cttaatccga aaatccgaaa tgcttcaaaa      660

tctgcaacct ttgagcactg atgtgttgct caaaggcaat gtgcgttgga gcgttccaga      720

tttgggggtt agggatgttc agcctgtaaa tatagtgcac atatttcaaa atctgaaaaa      780

aaaattgaaa tccaaaacac                                                  800


<210>  21
<211>  1062
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CDK4

<400>  21
aggtggggtg tgatgatctg tagagaagtg gggaccctga ggaaataatg agaggccatg       60

ttgggttaaa ggggattgaa aagtgagcat ttactctggt caggctgatg gacgtctgtg      120

ccacatcccg aactgaccgg gagatcaagg taaccctggt gtttgagcat gtagaccagg      180

acctaaggac atatctggac aaggcacccc caccaggctt gccagccgaa acgatcaagg      240

tgagtggggt tggtaggcat tgagaggtgg attgggacct ttgtagtaga accttctggg      300

atttcaggta tggtgcctag tttccagtgc atctgtacct cccctttgaa actaggatct      360

gatgcgccag tttctaaggt aagtaataac ttcgtatagc atacattata cgaagttatt      420

caaggttaga agacaggttt aaggagacca atagaaactg ggcttgtcga gacagagaag      480

actcttgcgt ttctgatagg cacctattgg tcttactgac atccactttg ccataacttc      540

gtatagcata cattatacga agttattttc tctccacaga ggcctagatt tccttcatgc      600

caattgcatc gttcaccgag atctgaagcc agagaacatt ctggtgacaa gtggtggaac      660

agtcaagctg gctgactttg gcctggccag aatctacagc taccagatgg cacttacacc      720

cgtggtcagt agaaagatgg taccaaaatg ggttctggtt gggagtagga gagtgattgc      780

ccgtagcaat tgagaagtca tgtgcttcat gtgttcagtc aagcaagttg tgtttcatgg      840

taacccatgg ggtccccatc cattcttcct attcccttta ggttgttaca ctctggtacc      900

gagctcccga agttcttctg cagtccacat atgcaacacc tgtggacatg tggagtgttg      960

gctgtatctt tgcagagatg tttcgtcgaa agtatgggac ccacataccc tggactacct     1020

tgaattcccc aaatcgcttg ttcataaacc acatccatac ct                        1062


<210>  22
<211>  1001
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  METTL16

<400>  22
acttgttcaa agtcacacag tgaggagacg acgagacagg aatttgagcc gagtcattta       60

atctctgtgc tccatatacg gatatgcagt gtttttggtg gaggattgta ctgattttca      120

taagctgtga ctgtgtgaaa cactcgtgta tgtgccactg ggatgagtgc tctgtgtaac      180

tgtgtgtgat ctccctggga ggttcggatg ggtatttggt tctgcctgtt tgccgtagag      240

cttgtacaac atagtgaatc ttactaatca gttttacttc cttttcttta ttcctcagcc      300

ttaattttaa agaccccgaa gcagtcagag ctctgacgtg tactctccta aggtaagtaa      360

taacttcgta tagcatacat tatacgaagt tattcaaggt tagaagacag gtttaaggag      420

accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta      480

ttggtcttac tgacatccac tttgccataa cttcgtatag catacattat acgaagttat      540

tttctctcca cagggaagat tttggacttt ctattgatat tccattggag agactaattc      600

ccacagttcc cttgagactc aactatattc actgggtaga agatctgatc ggtcaccagg      660

attctgacaa aagtactctc cgaagaggaa ttgacatagg tatatcattt taaattcttt      720

tttggctaaa cagttttcat aagttttgcg agatcaaatt ctttgtaaac ttttctactg      780

ggtatttgtt gttgtacaaa agaagctggc tacagataca ggttgagcat ccttaatccg      840

aaaatccgaa atgcttcaaa atctgcaacc tttgagcact gatgtgttgc tcaaaggcaa      900

tgtgcgttgg agcgttccag atttgggggt tagggatgtt cagcctgtaa atatagtgca      960

catatttcaa aatctgaaaa aaaaattgaa atccaaaaca c                         1001


<210>  23
<211>  387
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  METTL16-intron

<400>  23
tctttattcc tcagccttaa ttttaaagac cccgaagcag tcagagctct gacgtgtact       60

ctcctaaggt aagtaataac ttcgtatagc atacattata cgaagttatt caaggttaga      120

agacaggttt aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt      180

ttctgatagg cacctattgg tcttactgac atccactttg ccataacttc gtatagcata      240

cattatacga agttattttc tctccacagg gaagattttg gactttctat tgatattcca      300

ttggagagac taattcccac agttcccttg agactcaact atattcactg ggtagaagat      360

ctgatcggtc accaggattc tgacaaa                                          387


<210>  24
<211>  359
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CDK4-intron

<400>  24
ggtgcctagt ttccagtgca tctgtacctc ccctttgaaa ctaggatctg atgcgccagt       60

ttctaaggta agtaataact tcgtatagca tacattatac gaagttattc aaggttagaa      120

gacaggttta aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt      180

tctgataggc acctattggt cttactgaca tccactttgc cataacttcg tatagcatac      240

attatacgaa gttattttct ctccacagag gcctagattt ccttcatgcc aattgcatcg      300

ttcaccgaga tctgaagcca gagaacattc tggtgacaag tggtggaaca gtcaagctg       359


<210>  25
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting CD46

<400>  25
gauaaggguu uuuaccucga                                                   20


<210>  26
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting METTL16

<400>  26
ucugacgugu acucuccuaa                                                   20


<210>  27
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting Oct4/ Pou5F1 site#1

<400>  27
gcacuagccc cacuccaacc                                                   20


<210>  28
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting Oct4/ Pou5F1 site#2

<400>  28
accaccugga gggggcgaga                                                   20


<210>  29
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD46-DECAI genomic DNA fwd primer

<400>  29
tgcattccat tccttgtctc tg                                                22


<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD46-DECAI genomic DNA rev primer

<400>  30
aagacacttt ggaactgggg g                                                 21


<210>  31
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oct4-DECAI#1 fwd primer

<400>  31
ctctgaggtg tgggggatt                                                    19


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oct4-DECAI#1 rev primer

<400>  32
tgctccagct tctccttctc                                                   20


<210>  33
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oct4-DECAI#2 fwd primer

<400>  33
gacacctggc ttcggattt                                                    19


<210>  34
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oct4-DECAI#2 rev primer

<400>  34
ccccacagaa ctcatacgg                                                    19


<210>  35
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD46-DECAI RT-PCR fwd primer

<400>  35
ggtcaaatgt cgatttccag tagt                                              24


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD46-DECAI RT-PCR rev primer

<400>  36
gacactttgg aactggggga                                                   20


<210>  37
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAPDH RT-PCR fwd primer

<400>  37
gaaggtgaag gtcggagt                                                     18


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAPDH RT-PCR rev primer

<400>  38
gaagatggtg atgggatttc                                                   20


<210>  39
<211>  1271
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD46-DECAI donor sequence

<400>  39
ttaccaccac caccaccccc ctgcagtagt ttggtaaaaa ctaccaaaca aaattttaaa       60

aagcagccag tgagtgggga aaacaacaaa aacccgtatt acatttagga gggcagggac      120

aagtaagaat gacagccagc caagttttct taagaaaatg tggttacaat atggttatct      180

ctgttagtgc tgaaagaaaa ataaaacacc ctagaattct gtatgaagga gaatatatta      240

gttaacattt aaagtctttt aaaaaatgtt tttaacatct tgcattccat tccttgtctc      300

tgttcacact ggaaattact actttgtact actttttctg ctaaagcaga tatccattaa      360

ttctgaggtt tctctaattt tccagtggtc aaatgtcgat ttccagtagt cgaaaatgga      420

aaacagatat caggatttgg aaaaaaattt tactacaaag caacagttat gtttgaatgc      480

gataaggtaa gtaataactt cgtatagcat acattatacg aagttattca aggttagaag      540

acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac tcttgcgttt      600

ctgataggca cctattggtc ttactgacat ccactttgcc ataacttcgt atagcataca      660

ttatacgaag ttattttctc tccacagggt ttttacctcg atggcagcga cacaattgtc      720

tgtgacagta acagtacttg ggatccccca gttccaaagt gtcttaaagg tacaaaggtt      780

atcttttttc tgtcttggtt tgttattgtt gttgctgttc attttagact ttatttcttt      840

gatattaact atcagtcata caaaataact gaaaagaaac aattttagta tttaactctg      900

tcttgtattc atttctatgc cagatgaatg acacgaaatt cacataaaat tctgctgttg      960

tgattttttg tgcttttcca gggttcttag cacgttatgt acattgcatg ggtatatgct     1020

tttaatattt ttatgtataa aaagtgaatt acaacaactt tttggaattg aaacatgggc     1080

atttttatct aagtaagtca acaatggcat aattcatata aatgaaatga gagcaataac     1140

tcccaagtgg ttgatcttct aacattattt tgtttcctag tgctgcctcc atctagtaca     1200

aaacctccag ctttgagtca ttcaggttta gtagcttctt ccttatatgt cttcttcctt     1260

atatgttaca a                                                          1271


<210>  40
<211>  1000
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  METTL16-DECAI donor sequence

<400>  40
acttgttcaa agtcacacag tgaggagacg acgagacagg aatttgagcc gagtcattta       60

atctctgtgc tccatatacg gatatgcagt gtttttggtg gaggattgta ctgattttca      120

taagctgtga ctgtgtgaaa cactcgtgta tgtgccactg ggatgagtgc tctgtgtaac      180

tgtgtgtgat ctccctggga ggttcggatg ggtatttggt tctgcctgtt tgccgtagag      240

cttgtacaac atagtgaatc ttactaatca gttttacttc cttttcttta ttcctcagcc      300

ttaattttaa agaccccgaa gcagtcagag ctctgacgtg tactctccta aggtaagtaa      360

taacttcgta tagcatacat tatacgaagt tattcaaggt tagaagacag gtttaaggag      420

accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta      480

ttggtcttac tgacatccac tttgccataa cttcgtatag catacattat acgaagttat      540

tttctctcca cagggaagat tttggacttt ctattgatat tccattggag agactaattc      600

ccacagttcc cttgagactc aactatattc actgggtaga agatctgatc ggtcaccagg      660

attctgacaa aagtactctc cgaagaggaa ttgacatagg tatatcattt taaattcttt      720

tttggctaaa cagttttcat aagttttgcg agatcaaatt ctttgtaaac ttttctactg      780

ggtatttgtt gttgtacaaa agaagctggc tacagataca ggttgagcat ccttaatccg      840

aaaatccgaa atgcttcaaa atctgcaacc tttgagcact gatgtgttgc tcaaaggcaa      900

tgtgcgttgg agcgttccag atttgggggt tagggatgtt cagcctgtaa atatagtgca      960

catatttcaa aatctgaaaa aaaaattgaa atccaaaaca                           1000


<210>  41
<211>  1005
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oct4-DECAI#1 donor sequence

<400>  41
ccttcgcctc agtttctccc cccacctccc tctcctccac ccatccaggg ggcggggcca       60

gaggtcaagg ctagtgggtg ggactgggga gggagagagg ggttgagtag tcccttcgca      120

agccctcatt tcaccaggcc cccggcttgg ggcgccttcc ttccccatgg cgggacacct      180

ggcttcggat ttcgccttct cgccccctcc aggtggtgga ggtgatgggc caggggggcc      240

ggagccgggc tgggttgatc ctcggacctg gctaagcttc caaggccctc ctggagggcc      300

aggaatcggg ccgggggttg ggccaggctc tgaggtgtgg gggattcccc catgcccccc      360

gccgtatgag ttctgtgggg ggatggcgta ctgtgggccc caggtaagta ataacttcgt      420

atagcataca ttatacgaag ttattcaagg ttagaagaca ggtttaagga gaccaataga      480

aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta      540

ctgacatcca ctttgccata acttcgtata gcatacatta tacgaagtta ttttctctcc      600

acaggttgga gtggggctag tgccccaagg cggcttggag acctctcagc ctgagggcga      660

agcaggagtc ggggtggaga gcaactccga tggggcctcc ccggagccct gcaccgtcac      720

ccctggtgcc gtgaagctgg agaaggagaa gctggagcaa aacccggagg aggcaagtga      780

gcttcgacgg ggttggggtg tggggaggtg gtcatgacag ggcagcctga tggggaagtg      840

gtcacctgca gctgcccaga cctggcaccc aggagaggag caggcagggt cagctgccct      900

ggccagggag gggtgtgtat caactgctgg cagccctggc aggcaggggc caggtgggaa      960

gtggaagctg gatttcgaag agacaactgc cggtgagggc agagc                     1005


<210>  42
<211>  1005
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oct4-DECAI#2 donor sequence

<400>  42
gaggctacat tgagccatca ttgtactcca ctgcactcca gtctgggcaa caaagtgaga       60

ccctgtctta aaaaataaaa ataaaaaaag tttctgtggg ggacctgcac tgaggtcctg      120

gaggggcgcc agttgtgtct cccggttttc cccttccaca gacaccattg ccaccaccat      180

taggcaaaca tccttcgcct cagtttctcc ccccacctcc ctctcctcca cccatccagg      240

gggcggggcc agaggtcaag gctagtgggt gggactgggg agggagagag gggttgagta      300

gtcccttcgc aagccctcat ttcaccaggc ccccggcttg gggcgccttc cttccccatg      360

gcgggacacc tggcttcgga tttcgccttc tcgccccctc caggtaagta ataacttcgt      420

atagcataca ttatacgaag ttattcaagg ttagaagaca ggtttaagga gaccaataga      480

aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta      540

ctgacatcca ctttgccata acttcgtata gcatacatta tacgaagtta ttttctctcc      600

acaggtggtg gaggtgatgg gccagggggg ccggagccgg gctgggttga tcctcggacc      660

tggctaagct tccaaggccc tcctggaggg ccaggaatcg ggccgggggt tgggccaggc      720

tctgaggtgt gggggattcc cccatgcccc ccgccgtatg agttctgtgg ggggatggcg      780

tactgtgggc cccaggttgg agtggggcta gtgccccaag gcggcttgga gacctctcag      840

cctgagggcg aagcaggagt cggggtggag agcaactccg atggggcctc cccggagccc      900

tgcaccgtca cccctggtgc cgtgaagctg gagaaggaga agctggagca aaacccggag      960

gaggcaagtg agcttcgacg gggttggggt gtggggaggt ggtca                     1005


<210>  43
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  CDK4 guide RNA

<400>  43
cugaugcgcc aguuucuaag                                                   20


<210>  44
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  METTL16 guide RNA

<400>  44
ucugacgugu acucuccuaa                                                   20


