                         SEQUENCE LISTING

<110>  TriBiotica LLC
       Dunn, Ian
       Lawler, Matthew
 
<120>  Methods For Split-Protein Template Assembly By Proximity-Enhanced
        Reactivity

<130>  189156.00702 (3031)

<150>  62/424,689
<151>  2016-11-21

<160>  59

<170>  PatentIn version 3.5

<210>  1
<211>  88
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal fragment

<400>  1

Ala Pro Ile Val Thr Cys Arg Lys Leu Asp Gly Arg Glu Lys Pro Phe 
1               5                   10                  15      

Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly Leu 
            20                  25                  30          

Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp His 
        35                  40                  45              

Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu Trp 
    50                  55                  60                  

Glu Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala Glu Trp Ala Lys Asp 
65                  70                  75                  80  

Val Lys Thr Ser Gln Gln Lys Gly 
                85              


<210>  2
<211>  41
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C-terminal fragment

<400>  2

Gly Pro Thr Pro Ile Arg Val Val Tyr Ala Asn Ser Arg Gly Ala Val 
1               5                   10                  15      

Gln Tyr Cys Gly Val Met Thr His Ser Lys Val Asp Lys Asn Asn Gln 
            20                  25                  30          

Gly Lys Glu Phe Phe Glu Lys Cys Asp 
        35                  40      


<210>  3
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal fragment

<400>  3

Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly 
1               5                   10          


<210>  4
<211>  118
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C-terminal fragment

<400>  4

Arg Glu Lys Pro Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala 
1               5                   10                  15      

Arg Lys Ala Gly Leu Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr 
            20                  25                  30          

Phe Ala Gly Asp His Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala 
        35                  40                  45              

Asp Ala Ile Leu Trp Glu Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala 
    50                  55                  60                  

Glu Trp Ala Lys Asp Val Lys Thr Ser Gln Gln Lys Gly Gly Pro Thr 
65                  70                  75                  80  

Pro Ile Arg Val Val Tyr Ala Asn Ser Arg Gly Ala Val Gln Tyr Cys 
                85                  90                  95      

Gly Val Met Thr His Ser Lys Val Asp Lys Asn Asn Gln Gly Lys Glu 
            100                 105                 110         

Phe Phe Glu Lys Cys Asp 
        115             


<210>  5
<211>  37
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal fragment

<400>  5

Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      

Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          

Leu Thr Thr Gly Lys 
        35          


<210>  6
<211>  93
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C-terminal fragment

<400>  6

Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp His Ile Arg Trp Gly 
1               5                   10                  15      

Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu Trp Glu Tyr Pro Ile 
            20                  25                  30          

Tyr Trp Val Gly Lys Asn Ala Glu Trp Ala Lys Asp Val Lys Thr Ser 
        35                  40                  45              

Gln Gln Lys Gly Gly Pro Thr Pro Ile Arg Val Val Tyr Ala Asn Ser 
    50                  55                  60                  

Arg Gly Ala Val Gln Tyr Cys Gly Val Met Thr His Ser Lys Val Asp 
65                  70                  75                  80  

Lys Asn Asn Gln Gly Lys Glu Phe Phe Glu Lys Cys Asp 
                85                  90              


<210>  7
<211>  61
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal fragment

<400>  7

Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      

Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          

Leu Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp 
        35                  40                  45              

His Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp 
    50                  55                  60      


<210>  8
<211>  69
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C-terminal fragment

<400>  8

Ala Ile Leu Trp Glu Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala Glu 
1               5                   10                  15      

Trp Ala Lys Asp Val Lys Thr Ser Gln Gln Lys Gly Gly Pro Thr Pro 
            20                  25                  30          

Ile Arg Val Val Tyr Ala Asn Ser Arg Gly Ala Val Gln Tyr Cys Gly 
        35                  40                  45              

Val Met Thr His Ser Lys Val Asp Lys Asn Asn Gln Gly Lys Glu Phe 
    50                  55                  60                  

Phe Glu Lys Cys Asp 
65                  


<210>  9
<211>  73
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal fragment

<400>  9

Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      

Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          

Leu Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp 
        35                  40                  45              

His Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu 
    50                  55                  60                  

Trp Glu Tyr Pro Ile Tyr Trp Val Gly 
65                  70              


<210>  10
<211>  57
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C-terminal fragment

<400>  10

Lys Asn Ala Glu Trp Ala Lys Asp Val Lys Thr Ser Gln Gln Lys Gly 
1               5                   10                  15      

Gly Pro Thr Pro Ile Arg Val Val Tyr Ala Asn Ser Arg Gly Ala Val 
            20                  25                  30          

Gln Tyr Cys Gly Val Met Thr His Ser Lys Val Asp Lys Asn Asn Gln 
        35                  40                  45              

Gly Lys Glu Phe Phe Glu Lys Cys Asp 
    50                  55          


<210>  11
<211>  81
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal fragment

<400>  11

Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      

Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          

Leu Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp 
        35                  40                  45              

His Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu 
    50                  55                  60                  

Trp Glu Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala Glu Trp Ala Lys 
65                  70                  75                  80  

Asp 
    

<210>  12
<211>  49
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C-terminal fragment

<400>  12

Val Lys Thr Ser Gln Gln Lys Gly Gly Pro Thr Pro Ile Arg Val Val 
1               5                   10                  15      

Tyr Ala Asn Ser Arg Gly Ala Val Gln Tyr Cys Gly Val Met Thr His 
            20                  25                  30          

Ser Lys Val Asp Lys Asn Asn Gln Gly Lys Glu Phe Phe Glu Lys Cys 
        35                  40                  45              

Asp 
    

<210>  13
<211>  86
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal fragment

<400>  13

Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      

Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          

Leu Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp 
        35                  40                  45              

His Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu 
    50                  55                  60                  

Trp Glu Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala Glu Trp Ala Lys 
65                  70                  75                  80  

Asp Val Lys Thr Ser Gln 
                85      


<210>  14
<211>  44
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C-terminal fragment

<400>  14

Gln Lys Gly Gly Pro Thr Pro Ile Arg Val Val Tyr Ala Asn Ser Arg 
1               5                   10                  15      

Gly Ala Val Gln Tyr Cys Gly Val Met Thr His Ser Lys Val Asp Lys 
            20                  25                  30          

Asn Asn Gln Gly Lys Glu Phe Phe Glu Lys Cys Asp 
        35                  40                  


<210>  15
<211>  103
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal fragment

<400>  15

Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      

Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          

Leu Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp 
        35                  40                  45              

His Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu 
    50                  55                  60                  

Trp Glu Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala Glu Trp Ala Lys 
65                  70                  75                  80  

Asp Val Lys Thr Ser Gln Gln Lys Gly Gly Pro Thr Pro Ile Arg Val 
                85                  90                  95      

Val Tyr Ala Asn Ser Arg Gly 
            100             


<210>  16
<211>  27
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C-terminal fragment

<400>  16

Ala Val Gln Tyr Cys Gly Val Met Thr His Ser Lys Val Asp Lys Asn 
1               5                   10                  15      

Asn Gln Gly Lys Glu Phe Phe Glu Lys Cys Asp 
            20                  25          


<210>  17
<211>  119
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal fragment

<400>  17

Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      

Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          

Leu Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp 
        35                  40                  45              

His Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu 
    50                  55                  60                  

Trp Glu Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala Glu Trp Ala Lys 
65                  70                  75                  80  

Asp Val Lys Thr Ser Gln Gln Lys Gly Gly Pro Thr Pro Ile Arg Val 
                85                  90                  95      

Val Tyr Ala Asn Ser Arg Gly Ala Val Gln Tyr Cys Gly Val Met Thr 
            100                 105                 110         

His Ser Lys Val Asp Lys Asn 
        115                 


<210>  18
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C-terminal fragment

<400>  18

Asn Gln Gly Lys Glu Phe Phe Glu Lys Cys Asp 
1               5                   10      


<210>  19
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  oligonucleotide

<400>  19

Gly Ser Gly Ser Gly His His His His 
1               5                  


<210>  20
<211>  34
<212>  DNA
<213>  Artificial sequence

<220>
<223>  polynucleotide sequence

<400>  20
aagccactgt gtcctgaaga aaagcaaaga catc             34


<210>  21
<211>  22
<212>  PRT
<213>  Artificial sequence

<220>
<223>  peptide

<400>  21

Ser Tyr Ser Met Glu His Phe Arg Trp Gly Lys Pro Val Gly Gly Gly 
1               5                   10                  15      

Ser Ser Gly Gly Gly Cys 
            20          


<210>  22
<211>  22
<212>  PRT
<213>  Artificial sequence

<220>
<223>  peptide

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa is Nle 

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Phe can be D-phenylalanine

<400>  22

Ser Tyr Ser Xaa Glu His Phe Arg Trp Gly Lys Pro Val Gly Gly Gly 
1               5                   10                  15      

Ser Ser Gly Gly Gly Cys 
            20          


<210>  23
<211>  23
<212>  PRT
<213>  Artificial sequence

<220>
<223>  peptide

<220>
<221>   misc_feature
<222>  (17)..(17)
<223>  Phe can be D-phenylalanine

<400>  23

Cys Ser Gly Gly Gly Ser Ser Gly Gly Gly Ser Tyr Ser Met Glu His 
1               5                   10                  15      

Phe Arg Trp Gly Lys Pro Val
            20                  


<210>  24
<211>  23
<212>  PRT
<213>  Artificial sequence

<220>
<223>  peptide

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Xaa is Nle 

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Phe can be D-phenylalanine

<400>  24

Cys Ser Gly Gly Gly Ser Ser Gly Gly Gly Ser Tyr Ser Xaa Glu His 
1               5                   10                  15      


Phe Arg Trp Gly Lys Pro Val
            20                  


<210>  25
<211>  90
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal protein fragment

<400>  25

Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      

Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          

Leu Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp 
        35                  40                  45              

His Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu 
    50                  55                  60                  

Trp Glu Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala Glu Trp Ala Lys 
65                  70                  75                  80  

Asp Val Lys Thr Ser Gln Gln Lys Gly Cys 
                85                  90  


<210>  26
<211>  42
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C-terminal protein fragment

<400>  26

Cys Gly Pro Thr Pro Ile Arg Val Val Tyr Ala Asn Ser Arg Gly Ala 
1               5                   10                  15      

Val Gln Tyr Cys Gly Val Met Thr His Ser Lys Val Asp Lys Asn Asn 
            20                  25                  30          

Gln Gly Lys Glu Phe Phe Glu Lys Cys Asp 
        35                  40          


<210>  27
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Factor Xa protease cleavage site

<400>  27

Ile Glu Gly Arg 
1               


<210>  28
<211>  60
<212>  DNA
<213>  Artificial sequence

<220>
<223>  bottle haplomer nucleotide sequence

<400>  28
actcgagacg tctccttgtc tttgcttttc ttcaggacac agtggcgaga cgtctcgagt 60


<210>  29
<211>  51
<212>  DNA
<213>  Artificial sequence

<220>
<223>  bottle haplomer nucleotide sequence

<400>  29
actcgagacg tctccttcct gcccctcctc ctgctccgag acgtctcgag t       51


<210>  30
<211>  11
<212>  DNA
<213>  Artificial sequence

<220>
<223>  second haplomer nucleotide sequence

<400>  30
agctctcgag t                                    11


<210>  31
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  second haplomer nucleotide sequence

<400>  31
gacgtctcga gt                                   12


<210>  32
<211>  60
<212>  DNA
<213>  Artificial sequence

<220>
<223>  polynucleotide of the bottle haplomer nucleotide sequence

<400>  32
actcgagacg tctccttgtc tttgcttttc ttcaggacac agtggcgaga cgtctcgagt   60


<210>  33
<211>  157
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal fragment of the superfolder GFP

<400>  33

Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      

Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly Glu 
            20                  25                  30          

Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              

Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  

Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Arg 
65                  70                  75                  80  

His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      

Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         

Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             

Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 

Phe Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln 
145                 150                 155         


<210>  34
<211>  81
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C-terminal fragment of the superfolder GFP

<400>  34

Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Val Glu Asp 
1               5                   10                  15      

Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly 
            20                  25                  30          

Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser 
        35                  40                  45              

Val Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu 
    50                  55                  60                  

Glu Phe Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr 
65                  70                  75                  80  

Lys 
    

<210>  35
<211>  157
<212>  PRT
<213>  Artificial sequence

<220>
<223>  fragment of superfolder GFP (sfGFP)

<400>  35

Met Arg Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      

Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly Glu 
            20                  25                  30          

Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              

Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  

Thr Tyr Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  

His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      

Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         

Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             

Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 

Phe Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln 
145                 150                 155         


<210>  36
<211>  230
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal fragment of the Renilla luciferase

<400>  36

Met Ala Ser Lys Val Tyr Asp Pro Glu Gln Arg Lys Arg Met Ile Thr 
1               5                   10                  15      

Gly Pro Gln Trp Trp Ala Arg Cys Lys Gln Met Asn Val Leu Asp Ser 
            20                  25                  30          

Phe Ile Asn Tyr Tyr Asp Ser Glu Lys His Ala Glu Asn Ala Val Ile 
        35                  40                  45              

Phe Leu His Gly Asn Ala Ala Ser Ser Tyr Leu Trp Arg His Val Val 
    50                  55                  60                  

Pro His Ile Glu Pro Val Ala Arg Cys Ile Ile Pro Asp Leu Ile Gly 
65                  70                  75                  80  

Met Gly Lys Ser Gly Lys Ser Gly Asn Gly Ser Tyr Arg Leu Leu Asp 
                85                  90                  95      

His Tyr Lys Tyr Leu Thr Ala Trp Phe Glu Leu Leu Asn Leu Pro Lys 
            100                 105                 110         

Lys Ile Ile Phe Val Gly His Asp Trp Gly Ala Cys Leu Ala Phe His 
        115                 120                 125             

Tyr Ser Tyr Glu His Gln Asp Lys Ile Lys Ala Ile Val His Ala Glu 
    130                 135                 140                 

Ser Val Val Asp Val Ile Glu Ser Trp Asp Glu Trp Pro Asp Ile Glu 
145                 150                 155                 160 

Glu Asp Ile Ala Leu Ile Lys Ser Glu Glu Gly Glu Lys Met Val Leu 
                165                 170                 175     

Glu Asn Asn Phe Phe Val Glu Thr Met Leu Pro Ser Lys Ile Met Arg 
            180                 185                 190         

Lys Leu Glu Pro Glu Glu Phe Ala Ala Tyr Leu Glu Pro Phe Lys Glu 
        195                 200                 205             

Lys Gly Glu Val Arg Arg Pro Thr Leu Ser Trp Pro Arg Glu Ile Pro 
    210                 215                 220                 

Leu Val Lys Gly Gly Tyr 
225                 230 


<210>  37
<211>  83
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C-terminal fragment of the Renilla luciferase

<400>  37

Lys Pro Asp Val Val Gln Ile Val Arg Asn Tyr Asn Ala Tyr Leu Arg 
1               5                   10                  15      

Ala Ser Asp Asp Leu Pro Lys Met Phe Ile Glu Ser Asp Pro Gly Phe 
            20                  25                  30          

Phe Ser Asn Ala Ile Val Glu Gly Ala Lys Lys Phe Pro Asn Thr Glu 
        35                  40                  45              

Phe Val Lys Val Lys Gly Leu His Phe Ser Gln Glu Asp Ala Pro Asp 
    50                  55                  60                  

Glu Met Gly Lys Tyr Ile Lys Ser Phe Val Glu Arg Val Leu Lys Asn 
65                  70                  75                  80  

Glu Gln Glx 
            

<210>  38
<211>  229
<212>  PRT
<213>  Artificial sequence

<220>
<223>  fragment of Renilla luciferase

<400>  38

Met Ala Ser Lys Val Tyr Asp Pro Glu Gln Arg Lys Arg Met Ile Thr 
1               5                   10                  15      

Gly Pro Gln Trp Trp Ala Arg Cys Lys Gln Met Asn Val Leu Asp Ser 
            20                  25                  30          

Phe Ile Asn Tyr Tyr Asp Ser Glu Lys His Ala Glu Asn Ala Val Ile 
        35                  40                  45              

Phe Leu His Gly Asn Ala Ala Ser Ser Tyr Leu Trp Arg His Val Val 
    50                  55                  60                  

Pro His Ile Glu Pro Val Ala Arg Cys Ile Ile Pro Asp Leu Ile Gly 
65                  70                  75                  80  

Met Gly Lys Ser Gly Lys Ser Gly Asn Gly Ser Tyr Arg Leu Leu Asp 
                85                  90                  95      

His Tyr Lys Tyr Leu Thr Ala Trp Phe Glu Leu Leu Asn Leu Pro Lys 
            100                 105                 110         

Lys Ile Ile Phe Val Gly His Asp Trp Gly Ala Cys Leu Ala Phe His 
        115                 120                 125             

Tyr Ser Tyr Glu His Gln Asp Lys Ile Lys Ala Ile Val His Ala Glu 
    130                 135                 140                 

Ser Val Val Asp Val Ile Glu Ser Trp Asp Glu Trp Pro Asp Ile Glu 
145                 150                 155                 160 

Glu Asp Ile Ala Leu Ile Lys Ser Glu Glu Gly Glu Lys Met Val Leu 
                165                 170                 175     

Glu Asn Asn Phe Phe Val Glu Thr Met Leu Pro Ser Lys Ile Met Arg 
            180                 185                 190         

Lys Leu Glu Pro Glu Glu Phe Ala Ala Tyr Leu Glu Pro Phe Lys Glu 
        195                 200                 205             

Lys Gly Glu Val Arg Arg Pro Thr Leu Ser Trp Pro Arg Glu Ile Pro 
    210                 215                 220                 

Leu Val Lys Gly Gly 
225                 


<210>  39
<211>  82
<212>  PRT
<213>  Artificial sequence

<220>
<223>  fragment of Renilla luciferase

<400>  39

Lys Pro Asp Val Val Gln Ile Val Arg Asn Tyr Asn Ala Tyr Leu Arg 
1               5                   10                  15      

Ala Ser Asp Asp Leu Pro Lys Met Phe Ile Glu Ser Asp Pro Gly Phe 
            20                  25                  30          

Phe Ser Asn Ala Ile Val Glu Gly Ala Lys Lys Phe Pro Asn Thr Glu 
        35                  40                  45              

Phe Val Lys Val Lys Gly Leu His Phe Ser Gln Glu Asp Ala Pro Asp 
    50                  55                  60                  

Glu Met Gly Lys Tyr Ile Lys Ser Phe Val Glu Arg Val Leu Lys Asn 
65                  70                  75                  80  

Glu Gln 
        

<210>  40
<211>  34
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal fragment

<400>  40

Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      

Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          

Leu Thr 
        

<210>  41
<211>  96
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C-terminal fragment

<400>  41

Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp His Ile 
1               5                   10                  15      

Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu Trp Glu 
            20                  25                  30          

Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala Glu Trp Ala Lys Asp Val 
        35                  40                  45              

Lys Thr Ser Gln Gln Lys Gly Gly Pro Thr Pro Ile Arg Val Val Tyr 
    50                  55                  60                  

Ala Asn Ser Arg Gly Ala Val Gln Tyr Cys Gly Val Met Thr His Ser 
65                  70                  75                  80  

Lys Val Asp Lys Asn Asn Gln Gly Lys Glu Phe Phe Glu Lys Cys Asp 
                85                  90                  95      


<210>  42
<211>  93
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C-terminal fragment of the Gaussia princeps luciferase

<400>  42

Met Lys Pro Thr Glu Asn Asn Glu Asp Phe Asn Ile Val Ala Val Ala 
1               5                   10                  15      

Ser Asn Phe Ala Thr Thr Asp Leu Asp Ala Asp Arg Gly Lys Leu Pro 
            20                  25                  30          

Gly Lys Lys Leu Pro Leu Glu Val Leu Lys Glu Met Glu Ala Asn Ala 
        35                  40                  45              

Arg Lys Ala Gly Cys Thr Arg Gly Cys Leu Ile Cys Leu Ser His Ile 
    50                  55                  60                  

Lys Cys Thr Pro Lys Met Lys Lys Phe Ile Pro Gly Arg Cys His Thr 
65                  70                  75                  80  

Tyr Glu Gly Asp Lys Glu Ser Ala Gln Gly Gly Ile Gly 
                85                  90              


<210>  43
<211>  76
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C-terminal fragment of the Gaussia princeps luciferase

<400>  43

Glu Ala Ile Val Asp Ile Pro Glu Ile Pro Gly Phe Lys Asp Leu Glu 
1               5                   10                  15      

Pro Met Glu Gln Phe Ile Ala Gln Val Asp Leu Cys Val Asp Cys Thr 
            20                  25                  30          

Thr Gly Cys Leu Lys Gly Leu Ala Asn Val Gln Cys Ser Asp Leu Leu 
        35                  40                  45              

Lys Lys Trp Leu Pro Gln Arg Cys Ala Thr Phe Ala Ser Lys Ile Gln 
    50                  55                  60                  

Gly Gln Val Asp Lys Ile Lys Gly Ala Gly Gly Asp 
65                  70                  75      


<210>  44
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  enterokinase recognition signal

<400>  44

Asp Asp Asp Asp Lys 
1               5   


<210>  45
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  oligonucleotide

<400>  45
tttcttcagg acacagc                                            17


<210>  46
<211>  34
<212>  DNA
<213>  Artificial sequence

<220>
<223>  appended template tag

<400>  46
gatgtctttg cttttcttca ggacacagtg gctt                           34


<210>  47
<211>  89
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminal

<400>  47

Ala Pro Ile Val Thr Cys Arg Lys Leu Asp Gly Arg Glu Lys Pro Phe 
1               5                   10                  15      

Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly Leu 
            20                  25                  30          

Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp His 
        35                  40                  45              

Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu Trp 
    50                  55                  60                  

Glu Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala Glu Trp Ala Lys Asp 
65                  70                  75                  80  

Val Lys Thr Ser Gln Gln Lys Gly Cys 
                85                  


<210>  48
<211>  35
<212>  RNA
<213>  Artificial sequence

<220>
<223>  synthetic 35-mer corresponding to sarcin-ricin loop in 28S RNA

<400>  48
gguaauccug cucaguacga gaggaaccgc agguu                  35


<210>  49
<211>  55
<212>  DNA
<213>  Artificial sequence

<220>
<223>  scrambled oligonucleotide

<400>  49
taactgtcaa aagccacaag cggaataatg acttcccagg gatagatcaa aaagc     55


<210>  50
<211>  130
<212>  PRT
<213>  Hirsutellin A

<400>  50

Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      

Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          

Leu Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp 
        35                  40                  45              

His Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu 
    50                  55                  60                  

Trp Glu Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala Glu Trp Ala Lys 
65                  70                  75                  80  

Asp Val Lys Thr Ser Gln Gln Lys Gly Gly Pro Thr Pro Ile Arg Val 
                85                  90                  95

Val Tyr Ala Asn Ser Arg Gly Ala Val Gln Tyr Cys Gly Val Met Thr 
            100                 105                 110

His Ser Lys Val Asp Lys Asn Asn Gln Gly Lys Glu Phe Phe Glu Lys 
        115                 120                 125

Cys Asp
    130


<210>  51
<211>  34
<212>  PRT
<213>  Hirsutellin A dithreonine site N-terminal fragment

<400>  51

Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      

Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          

Leu Thr 


<210>  52
<211>  89
<212>  PRT
<213>  Hirsutellin A diglycine site N-terminal fragment 

<400>  52

Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      

Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          

Leu Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp 
        35                  40                  45              

His Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu 
    50                  55                  60                  

Trp Glu Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala Glu Trp Ala Lys 
65                  70                  75                  80  

Asp Val Lys Thr Ser Gln Gln Lys Gly 
                85                  


<210>  53
<211>  167
<212>  PRT
<213>  sfGFP N-terminal fragment

<400>  53

Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val
1               5                   10                  15      

Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly Glu 
            20                  25                  30          

Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              

Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  

Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Arg 
65                  70                  75                  80  

His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95

Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110

Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125

Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140

Phe Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Gly Gly Ser 
145                 150                 155                 160

Gly His His His His His His
                165


<210>  54
<211>  92
<212>  PRT
<213>  sfGFP C-terminal fragment

<400>  54

Met His His His His His His Gly Gly Ser Gly Lys Asn Gly Ile Lys 
1               5                   10                  15      

Ala Asn Phe Lys Ile Arg His Asn Val Glu Asp Gly Ser Val Gln Leu 
            20                  25                  30          

Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 
        35                  40                  45              

Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Val Leu Ser Lys Asp 
    50                  55                  60                  

Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala 
65                  70                  75                  80  

Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys
                85                  90     



<210>  55
<211>  34
<212>  PRT
<213>  S. cerevisiae ubiquitin amino acids 1-34

<400>  55

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu
1               5                   10                  15      

Val Glu Ser Ser Asp Thr Ile Asp Asn Val Lys Ser Lys Ile Gln Asp 
            20                  25                  30          

Lys Glu

<210>  56
<211>  42
<212>  PRT
<213>  S. cerevisiae ubiquitin amino acids 35-76

<400>  56

Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln Leu 
1               5                   10                  15      

Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser Thr 
            20                  25                  30          

Leu His Leu Val Leu Arg Leu Arg Gly Gly
        35                  40

<210>  57
<211>  15
<212>  PRT
<213>  serine-glycine linker 

<400>  57

Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly  
1               5                   10                  15      


<210>  58
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  HPV-B2-TrisAm 

<400>  58
tttgacgtct cgagt                       15


<210>  59
<211>  56
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Lk-SP-haplomer 

<400>  59
taactgtcaa aagccactgt gtcctgaaga aaagcaaaga catctggaca aaaagc     56
