                         SEQUENCE LISTING

<110>  Centre National de la Recherche Scientifique (CNRS)
       Universit Pierre et Marie Curie (UPMC)
       FICKO-BLEAN, Elizabeth
       LAROCQUE, Robert
       CORREC, Galle
       CZJZEK, Mirjam
       MICHEL, Gurvan
 
<120>  Alpha-1,3-(3,6-ANHYDRO)-D-GALACTOSIDASES ET LEUR UTILISATION POUR
        HYDROLYSER DES POLYSACCHARIDES

<130>  BNT222046PC00

<150>  FR1661157
<151>  2016-11-17

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  2082
<212>  DNA
<213>  Zobellia_3152


<220>
<221>  CDS
<222>  (1)..(2082)

<400>  1
atg aaa aac aat agc aca gta gct aaa agc acc ctt gta ctc ttg gtt         48
Met Lys Asn Asn Ser Thr Val Ala Lys Ser Thr Leu Val Leu Leu Val           
1               5                   10                  15                

gtc acc tgt ttg acc gca ttc aaa ggc ctt gcc ttt gac tcc att agc         96
Val Thr Cys Leu Thr Ala Phe Lys Gly Leu Ala Phe Asp Ser Ile Ser           
            20                  25                  30                    

cca gac cct att gtt ttg gag aac gga aaa ttg aat atc aac atc gat        144
Pro Asp Pro Ile Val Leu Glu Asn Gly Lys Leu Asn Ile Asn Ile Asp           
        35                  40                  45                        

tct aaa aca gga tgc ttt tcg gtt acc gaa aaa act tcg ggc cac gtt        192
Ser Lys Thr Gly Cys Phe Ser Val Thr Glu Lys Thr Ser Gly His Val           
    50                  55                  60                            

tgg aaa tca gac cca tgg gaa aat gcg gcc ggt tta ctg acc ctg acc        240
Trp Lys Ser Asp Pro Trp Glu Asn Ala Ala Gly Leu Leu Thr Leu Thr           
65                  70                  75                  80            

gat tcc aaa gga aaa aag cag acc gtc aat att tca aag agc aaa aaa        288
Asp Ser Lys Gly Lys Lys Gln Thr Val Asn Ile Ser Lys Ser Lys Lys           
                85                  90                  95                

atc gag gtt tcc aaa acc gca aaa aat acg gta tcc cta aaa ttt atc        336
Ile Glu Val Ser Lys Thr Ala Lys Asn Thr Val Ser Leu Lys Phe Ile           
            100                 105                 110                   

gac ccg gtt ttt gaa gac ggc tcc gtt gca aaa ggc gtc tct atc gct        384
Asp Pro Val Phe Glu Asp Gly Ser Val Ala Lys Gly Val Ser Ile Ala           
        115                 120                 125                       

acg gaa ttg agg cta gac ccc aac aac gcc caa tta gac gtt gaa gtg        432
Thr Glu Leu Arg Leu Asp Pro Asn Asn Ala Gln Leu Asp Val Glu Val           
    130                 135                 140                           

acg gaa cac cgt tcg ggc aat ttt acc ctc tac gac ctg cgt tat ccc        480
Thr Glu His Arg Ser Gly Asn Phe Thr Leu Tyr Asp Leu Arg Tyr Pro           
145                 150                 155                 160           

gcc agg gcc ttc agt tta aaa acc gat gag gac aaa ggg gcg gcg gta        528
Ala Arg Ala Phe Ser Leu Lys Thr Asp Glu Asp Lys Gly Ala Ala Val           
                165                 170                 175               

atc cca caa aag cag ggc gtt att tgt cct tca tac atc ttt ccc atg        576
Ile Pro Gln Lys Gln Gly Val Ile Cys Pro Ser Tyr Ile Phe Pro Met           
            180                 185                 190                   

aac ggg gga cgt ttt tgc aaa tgg gac gac gcc acc tac aac aac aaa        624
Asn Gly Gly Arg Phe Cys Lys Trp Asp Asp Ala Thr Tyr Asn Asn Lys           
        195                 200                 205                       

tcg caa ggt tct tta gaa ctg ttc aac aac ggt acc gga ctc acc atg        672
Ser Gln Gly Ser Leu Glu Leu Phe Asn Asn Gly Thr Gly Leu Thr Met           
    210                 215                 220                           

cct tgg tgg ggt acc tat aat gaa aaa tcg gcc gtt atg ggc att gta        720
Pro Trp Trp Gly Thr Tyr Asn Glu Lys Ser Ala Val Met Gly Ile Val           
225                 230                 235                 240           

gat gtt tcg gcc cgt ccg cac atg caa tac aac atc aac aat aac gga        768
Asp Val Ser Ala Arg Pro His Met Gln Tyr Asn Ile Asn Asn Asn Gly           
                245                 250                 255               

caa tac ctt ttc aat gcc aag gga gta atg tct ccc tac cag cgt att        816
Gln Tyr Leu Phe Asn Ala Lys Gly Val Met Ser Pro Tyr Gln Arg Ile           
            260                 265                 270                   

gta ttt ctt gat ccc att tgg aaa ttg gac caa gaa aag gga aaa atg        864
Val Phe Leu Asp Pro Ile Trp Lys Leu Asp Gln Glu Lys Gly Lys Met           
        275                 280                 285                       

aga ata agc tac cat ttt att ccc gga ggt gat tac gtt gac atg gcc        912
Arg Ile Ser Tyr His Phe Ile Pro Gly Gly Asp Tyr Val Asp Met Ala           
    290                 295                 300                           

aaa gtt tac caa aaa gag gcc aag gca cgt ggc cac ttt gtt tcg ctc        960
Lys Val Tyr Gln Lys Glu Ala Lys Ala Arg Gly His Phe Val Ser Leu           
305                 310                 315                 320           

caa gaa aaa tta aag cga aac cca aac gtc aac aaa ctt ccc ggc gct       1008
Gln Glu Lys Leu Lys Arg Asn Pro Asn Val Asn Lys Leu Pro Gly Ala           
                325                 330                 335               

atc tat ttc ggc att tac gga ggg tat ccg cat tac gtg aac atg ccc       1056
Ile Tyr Phe Gly Ile Tyr Gly Gly Tyr Pro His Tyr Val Asn Met Pro           
            340                 345                 350                   

ggc atg gcc ttt act ttc gac gaa tta aaa aac atc ata aaa acc att       1104
Gly Met Ala Phe Thr Phe Asp Glu Leu Lys Asn Ile Ile Lys Thr Ile           
        355                 360                 365                       

cac gat gat tta agg gta gac aag gcc ttt gtt cat gcg tgg ggc act       1152
His Asp Asp Leu Arg Val Asp Lys Ala Phe Val His Ala Trp Gly Thr           
    370                 375                 380                           

ttt tcg aat ttc gtt cct cac aac tac cct att agc gaa gcc ttg gga       1200
Phe Ser Asn Phe Val Pro His Asn Tyr Pro Ile Ser Glu Ala Leu Gly           
385                 390                 395                 400           

ggt cct gaa aaa ttg aaa gct gcg gta gat ttg gct aaa tct tac ggg       1248
Gly Pro Glu Lys Leu Lys Ala Ala Val Asp Leu Ala Lys Ser Tyr Gly           
                405                 410                 415               

tat ttg tat tcg tct tac cac gcc tat tcg ccc atg ctg gag aac gac       1296
Tyr Leu Tyr Ser Ser Tyr His Ala Tyr Ser Pro Met Leu Glu Asn Asp           
            420                 425                 430                   

ccg aat ttc aca acg gac ctc atg caa cgc gat gcc gaa gga aaa ctg       1344
Pro Asn Phe Thr Thr Asp Leu Met Gln Arg Asp Ala Glu Gly Lys Leu           
        435                 440                 445                       

atg aat acc ggt agc cgt tgg gca cgt gtt gac cct aaa ttc caa aaa       1392
Met Asn Thr Gly Ser Arg Trp Ala Arg Val Asp Pro Lys Phe Gln Lys           
    450                 455                 460                           

gga ttg gcc cag aaa aat att gaa aag gaa att tca tac ttg ggc cta       1440
Gly Leu Ala Gln Lys Asn Ile Glu Lys Glu Ile Ser Tyr Leu Gly Leu           
465                 470                 475                 480           

gag gca gat ata acc gat att acc ttt gcc gcg tat cgt gag aac gga       1488
Glu Ala Asp Ile Thr Asp Ile Thr Phe Ala Ala Tyr Arg Glu Asn Gly           
                485                 490                 495               

aaa gaa ggt cgt att gaa ctg gcc aaa tac atc gat agt ttc aac ctt       1536
Lys Glu Gly Arg Ile Glu Leu Ala Lys Tyr Ile Asp Ser Phe Asn Leu           
            500                 505                 510                   

gtc aac gga acc gag cac ggc caa gag caa tgg atc ccc tac ttt gat       1584
Val Asn Gly Thr Glu His Gly Gln Glu Gln Trp Ile Pro Tyr Phe Asp           
        515                 520                 525                       

atg ttc gaa gga atg acc tat ttg gaa gac cgc cca ctt tcg gtc atc       1632
Met Phe Glu Gly Met Thr Tyr Leu Glu Asp Arg Pro Leu Ser Val Ile           
    530                 535                 540                           

tca cac cct gcc ccg ctt ttc aat tta gtg tac cat gaa gcg att gcc       1680
Ser His Pro Ala Pro Leu Phe Asn Leu Val Tyr His Glu Ala Ile Ala           
545                 550                 555                 560           

aat ttc ggt aag atc caa gac ccc gat aat gag gtt acc gct aac ggt       1728
Asn Phe Gly Lys Ile Gln Asp Pro Asp Asn Glu Val Thr Ala Asn Gly           
                565                 570                 575               

gat ttt aga atc aaa gcc ttg aga agc atg tta ttt ggc agg ggc aca       1776
Asp Phe Arg Ile Lys Ala Leu Arg Ser Met Leu Phe Gly Arg Gly Thr           
            580                 585                 590                   

aca atc ttc ttt gct ccc tat gaa ttt gaa ggc atg cgc ccc atg ata       1824
Thr Ile Phe Phe Ala Pro Tyr Glu Phe Glu Gly Met Arg Pro Met Ile           
        595                 600                 605                       

gaa atg gca agg gac tta gta agt ccg gtt cac aaa gaa acc ttt tat       1872
Glu Met Ala Arg Asp Leu Val Ser Pro Val His Lys Glu Thr Phe Tyr           
    610                 615                 620                           

tca gag tta aaa agc cat gaa tac ctc agc gcc gat tac aag gta caa       1920
Ser Glu Leu Lys Ser His Glu Tyr Leu Ser Ala Asp Tyr Lys Val Gln           
625                 630                 635                 640           

cga agc cgt ttt tcc agc gga acc gaa gtc att gca aat tta ggt ccc       1968
Arg Ser Arg Phe Ser Ser Gly Thr Glu Val Ile Ala Asn Leu Gly Pro           
                645                 650                 655               

gta gcc caa aaa atc gaa gga ggc att agc att cct ggc tac ggc tat       2016
Val Ala Gln Lys Ile Glu Gly Gly Ile Ser Ile Pro Gly Tyr Gly Tyr           
            660                 665                 670                   

cga ata caa atg aag gat ggc agt ctt aaa acc ggt cat ttc caa gtg       2064
Arg Ile Gln Met Lys Asp Gly Ser Leu Lys Thr Gly His Phe Gln Val           
        675                 680                 685                       

agt tta cat atg gat taa                                               2082
Ser Leu His Met Asp                                                       
    690                                                                   


<210>  2
<211>  693
<212>  PRT
<213>  Zobellia_3152

<400>  2

Met Lys Asn Asn Ser Thr Val Ala Lys Ser Thr Leu Val Leu Leu Val 
1               5                   10                  15      


Val Thr Cys Leu Thr Ala Phe Lys Gly Leu Ala Phe Asp Ser Ile Ser 
            20                  25                  30          


Pro Asp Pro Ile Val Leu Glu Asn Gly Lys Leu Asn Ile Asn Ile Asp 
        35                  40                  45              


Ser Lys Thr Gly Cys Phe Ser Val Thr Glu Lys Thr Ser Gly His Val 
    50                  55                  60                  


Trp Lys Ser Asp Pro Trp Glu Asn Ala Ala Gly Leu Leu Thr Leu Thr 
65                  70                  75                  80  


Asp Ser Lys Gly Lys Lys Gln Thr Val Asn Ile Ser Lys Ser Lys Lys 
                85                  90                  95      


Ile Glu Val Ser Lys Thr Ala Lys Asn Thr Val Ser Leu Lys Phe Ile 
            100                 105                 110         


Asp Pro Val Phe Glu Asp Gly Ser Val Ala Lys Gly Val Ser Ile Ala 
        115                 120                 125             


Thr Glu Leu Arg Leu Asp Pro Asn Asn Ala Gln Leu Asp Val Glu Val 
    130                 135                 140                 


Thr Glu His Arg Ser Gly Asn Phe Thr Leu Tyr Asp Leu Arg Tyr Pro 
145                 150                 155                 160 


Ala Arg Ala Phe Ser Leu Lys Thr Asp Glu Asp Lys Gly Ala Ala Val 
                165                 170                 175     


Ile Pro Gln Lys Gln Gly Val Ile Cys Pro Ser Tyr Ile Phe Pro Met 
            180                 185                 190         


Asn Gly Gly Arg Phe Cys Lys Trp Asp Asp Ala Thr Tyr Asn Asn Lys 
        195                 200                 205             


Ser Gln Gly Ser Leu Glu Leu Phe Asn Asn Gly Thr Gly Leu Thr Met 
    210                 215                 220                 


Pro Trp Trp Gly Thr Tyr Asn Glu Lys Ser Ala Val Met Gly Ile Val 
225                 230                 235                 240 


Asp Val Ser Ala Arg Pro His Met Gln Tyr Asn Ile Asn Asn Asn Gly 
                245                 250                 255     


Gln Tyr Leu Phe Asn Ala Lys Gly Val Met Ser Pro Tyr Gln Arg Ile 
            260                 265                 270         


Val Phe Leu Asp Pro Ile Trp Lys Leu Asp Gln Glu Lys Gly Lys Met 
        275                 280                 285             


Arg Ile Ser Tyr His Phe Ile Pro Gly Gly Asp Tyr Val Asp Met Ala 
    290                 295                 300                 


Lys Val Tyr Gln Lys Glu Ala Lys Ala Arg Gly His Phe Val Ser Leu 
305                 310                 315                 320 


Gln Glu Lys Leu Lys Arg Asn Pro Asn Val Asn Lys Leu Pro Gly Ala 
                325                 330                 335     


Ile Tyr Phe Gly Ile Tyr Gly Gly Tyr Pro His Tyr Val Asn Met Pro 
            340                 345                 350         


Gly Met Ala Phe Thr Phe Asp Glu Leu Lys Asn Ile Ile Lys Thr Ile 
        355                 360                 365             


His Asp Asp Leu Arg Val Asp Lys Ala Phe Val His Ala Trp Gly Thr 
    370                 375                 380                 


Phe Ser Asn Phe Val Pro His Asn Tyr Pro Ile Ser Glu Ala Leu Gly 
385                 390                 395                 400 


Gly Pro Glu Lys Leu Lys Ala Ala Val Asp Leu Ala Lys Ser Tyr Gly 
                405                 410                 415     


Tyr Leu Tyr Ser Ser Tyr His Ala Tyr Ser Pro Met Leu Glu Asn Asp 
            420                 425                 430         


Pro Asn Phe Thr Thr Asp Leu Met Gln Arg Asp Ala Glu Gly Lys Leu 
        435                 440                 445             


Met Asn Thr Gly Ser Arg Trp Ala Arg Val Asp Pro Lys Phe Gln Lys 
    450                 455                 460                 


Gly Leu Ala Gln Lys Asn Ile Glu Lys Glu Ile Ser Tyr Leu Gly Leu 
465                 470                 475                 480 


Glu Ala Asp Ile Thr Asp Ile Thr Phe Ala Ala Tyr Arg Glu Asn Gly 
                485                 490                 495     


Lys Glu Gly Arg Ile Glu Leu Ala Lys Tyr Ile Asp Ser Phe Asn Leu 
            500                 505                 510         


Val Asn Gly Thr Glu His Gly Gln Glu Gln Trp Ile Pro Tyr Phe Asp 
        515                 520                 525             


Met Phe Glu Gly Met Thr Tyr Leu Glu Asp Arg Pro Leu Ser Val Ile 
    530                 535                 540                 


Ser His Pro Ala Pro Leu Phe Asn Leu Val Tyr His Glu Ala Ile Ala 
545                 550                 555                 560 


Asn Phe Gly Lys Ile Gln Asp Pro Asp Asn Glu Val Thr Ala Asn Gly 
                565                 570                 575     


Asp Phe Arg Ile Lys Ala Leu Arg Ser Met Leu Phe Gly Arg Gly Thr 
            580                 585                 590         


Thr Ile Phe Phe Ala Pro Tyr Glu Phe Glu Gly Met Arg Pro Met Ile 
        595                 600                 605             


Glu Met Ala Arg Asp Leu Val Ser Pro Val His Lys Glu Thr Phe Tyr 
    610                 615                 620                 


Ser Glu Leu Lys Ser His Glu Tyr Leu Ser Ala Asp Tyr Lys Val Gln 
625                 630                 635                 640 


Arg Ser Arg Phe Ser Ser Gly Thr Glu Val Ile Ala Asn Leu Gly Pro 
                645                 650                 655     


Val Ala Gln Lys Ile Glu Gly Gly Ile Ser Ile Pro Gly Tyr Gly Tyr 
            660                 665                 670         


Arg Ile Gln Met Lys Asp Gly Ser Leu Lys Thr Gly His Phe Gln Val 
        675                 680                 685             


Ser Leu His Met Asp 
    690             


<210>  3
<211>  677
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Zobellia_3152 recombinante

<400>  3

Met Gly Ser Ser His His His His His His Gly Ser Leu Glu Ala Ser 
1               5                   10                  15      


Pro Asp Pro Ile Val Leu Glu Asn Gly Lys Leu Asn Ile Asn Ile Asp 
            20                  25                  30          


Ser Lys Thr Gly Cys Phe Ser Val Thr Glu Lys Thr Ser Gly His Val 
        35                  40                  45              


Trp Lys Ser Asp Pro Trp Glu Asn Ala Ala Gly Leu Leu Thr Leu Thr 
    50                  55                  60                  


Asp Ser Lys Gly Lys Lys Gln Thr Val Asn Ile Ser Lys Ser Lys Lys 
65                  70                  75                  80  


Ile Glu Val Ser Lys Thr Ala Lys Asn Thr Val Ser Leu Lys Phe Ile 
                85                  90                  95      


Asp Pro Val Phe Glu Asp Gly Ser Val Ala Lys Gly Val Ser Ile Ala 
            100                 105                 110         


Thr Glu Leu Arg Leu Asp Pro Asn Asn Ala Gln Leu Asp Val Glu Val 
        115                 120                 125             


Thr Glu His Arg Ser Gly Asn Phe Thr Leu Tyr Asp Leu Arg Tyr Pro 
    130                 135                 140                 


Ala Arg Ala Phe Ser Leu Lys Thr Asp Glu Asp Lys Gly Ala Ala Val 
145                 150                 155                 160 


Ile Pro Gln Lys Gln Gly Val Ile Cys Pro Ser Tyr Ile Phe Pro Met 
                165                 170                 175     


Asn Gly Gly Arg Phe Cys Lys Trp Asp Asp Ala Thr Tyr Asn Asn Lys 
            180                 185                 190         


Ser Gln Gly Ser Leu Glu Leu Phe Asn Asn Gly Thr Gly Leu Thr Met 
        195                 200                 205             


Pro Trp Trp Gly Thr Tyr Asn Glu Lys Ser Ala Val Met Gly Ile Val 
    210                 215                 220                 


Asp Val Ser Ala Arg Pro His Met Gln Tyr Asn Ile Asn Asn Asn Gly 
225                 230                 235                 240 


Gln Tyr Leu Phe Asn Ala Lys Gly Val Met Ser Pro Tyr Gln Arg Ile 
                245                 250                 255     


Val Phe Leu Asp Pro Ile Trp Lys Leu Asp Gln Glu Lys Gly Lys Met 
            260                 265                 270         


Arg Ile Ser Tyr His Phe Ile Pro Gly Gly Asp Tyr Val Asp Met Ala 
        275                 280                 285             


Lys Val Tyr Gln Lys Glu Ala Lys Ala Arg Gly His Phe Val Ser Leu 
    290                 295                 300                 


Gln Glu Lys Leu Lys Arg Asn Pro Asn Val Asn Lys Leu Pro Gly Ala 
305                 310                 315                 320 


Ile Tyr Phe Gly Ile Tyr Gly Gly Tyr Pro His Tyr Val Asn Met Pro 
                325                 330                 335     


Gly Met Ala Phe Thr Phe Asp Glu Leu Lys Asn Ile Ile Lys Thr Ile 
            340                 345                 350         


His Asp Asp Leu Arg Val Asp Lys Ala Phe Val His Ala Trp Gly Thr 
        355                 360                 365             


Phe Ser Asn Phe Val Pro His Asn Tyr Pro Ile Ser Glu Ala Leu Gly 
    370                 375                 380                 


Gly Pro Glu Lys Leu Lys Ala Ala Val Asp Leu Ala Lys Ser Tyr Gly 
385                 390                 395                 400 


Tyr Leu Tyr Ser Ser Tyr His Ala Tyr Ser Pro Met Leu Glu Asn Asp 
                405                 410                 415     


Pro Asn Phe Thr Thr Asp Leu Met Gln Arg Asp Ala Glu Gly Lys Leu 
            420                 425                 430         


Met Asn Thr Gly Ser Arg Trp Ala Arg Val Asp Pro Lys Phe Gln Lys 
        435                 440                 445             


Gly Leu Ala Gln Lys Asn Ile Glu Lys Glu Ile Ser Tyr Leu Gly Leu 
    450                 455                 460                 


Glu Ala Asp Ile Thr Asp Ile Thr Phe Ala Ala Tyr Arg Glu Asn Gly 
465                 470                 475                 480 


Lys Glu Gly Arg Ile Glu Leu Ala Lys Tyr Ile Asp Ser Phe Asn Leu 
                485                 490                 495     


Val Asn Gly Thr Glu His Gly Gln Glu Gln Trp Ile Pro Tyr Phe Asp 
            500                 505                 510         


Met Phe Glu Gly Met Thr Tyr Leu Glu Asp Arg Pro Leu Ser Val Ile 
        515                 520                 525             


Ser His Pro Ala Pro Leu Phe Asn Leu Val Tyr His Glu Ala Ile Ala 
    530                 535                 540                 


Asn Phe Gly Lys Ile Gln Asp Pro Asp Asn Glu Val Thr Ala Asn Gly 
545                 550                 555                 560 


Asp Phe Arg Ile Lys Ala Leu Arg Ser Met Leu Phe Gly Arg Gly Thr 
                565                 570                 575     


Thr Ile Phe Phe Ala Pro Tyr Glu Phe Glu Gly Met Arg Pro Met Ile 
            580                 585                 590         


Glu Met Ala Arg Asp Leu Val Ser Pro Val His Lys Glu Thr Phe Tyr 
        595                 600                 605             


Ser Glu Leu Lys Ser His Glu Tyr Leu Ser Ala Asp Tyr Lys Val Gln 
    610                 615                 620                 


Arg Ser Arg Phe Ser Ser Gly Thr Glu Val Ile Ala Asn Leu Gly Pro 
625                 630                 635                 640 


Val Ala Gln Lys Ile Glu Gly Gly Ile Ser Ile Pro Gly Tyr Gly Tyr 
                645                 650                 655     


Arg Ile Gln Met Lys Asp Gly Ser Leu Lys Thr Gly His Phe Gln Val 
            660                 665                 670         


Ser Leu His Met Asp 
        675         


<210>  4
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  4
ttttttgtcg acagcccaga ccctattgtt ttggag                                 36


<210>  5
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  5
ttttttctgc agttaatcca tatgtaaact cacttggaa                              39


