                         SEQUENCE LISTING

<110>  Lysando AG
 
<120>  New antimicrobial agents against Staphylococcus aureus

<130>  LYS-043 PCT

<150>  EP16199528.7
<151>  2016-11-18

<160>  111   

<170>  PatentIn version 3.5

<210>  1
<211>  93
<212>  PRT
<213>  Unknown

<220>
<223>  CHAP domain of  LysK endolysin

<400>  1

Ile Asp Ala Asp Gly Tyr Tyr His Ala Gln Cys Gln Asp Leu Ile Thr 
1               5                   10                  15      


Asp Tyr Val Leu Trp Leu Thr Asp Asn Lys Val Arg Thr Trp Gly Asn 
            20                  25                  30          


Ala Lys Asp Gln Ile Lys Gln Ser Tyr Gly Thr Gly Phe Lys Ile His 
        35                  40                  45              


Glu Asn Lys Pro Ser Thr Val Pro Lys Lys Gly Trp Ile Ala Val Phe 
    50                  55                  60                  


Thr Ser Gly Ser Tyr Glu Gln Trp Gly His Ile Gly Ile Val Tyr Asp 
65                  70                  75                  80  


Gly Gly Asn Thr Ser Thr Phe Thr Ile Leu Glu Gln Asn 
                85                  90              


<210>  2
<211>  177
<212>  PRT
<213>  unknown

<220>
<223>  N-terminus of LysK endolysin including CHAP domain; w/o 
       N-terminal methionine

<400>  2

Ala Lys Thr Gln Ala Glu Ile Asn Lys Arg Leu Asp Ala Tyr Ala Lys 
1               5                   10                  15      


Gly Thr Val Asp Ser Pro Tyr Arg Val Lys Lys Ala Thr Ser Tyr Asp 
            20                  25                  30          


Pro Ser Phe Gly Val Met Glu Ala Gly Ala Ile Asp Ala Asp Gly Tyr 
        35                  40                  45              


Tyr His Ala Gln Cys Gln Asp Leu Ile Thr Asp Tyr Val Leu Trp Leu 
    50                  55                  60                  


Thr Asp Asn Lys Val Arg Thr Trp Gly Asn Ala Lys Asp Gln Ile Lys 
65                  70                  75                  80  


Gln Ser Tyr Gly Thr Gly Phe Lys Ile His Glu Asn Lys Pro Ser Thr 
                85                  90                  95      


Val Pro Lys Lys Gly Trp Ile Ala Val Phe Thr Ser Gly Ser Tyr Glu 
            100                 105                 110         


Gln Trp Gly His Ile Gly Ile Val Tyr Asp Gly Gly Asn Thr Ser Thr 
        115                 120                 125             


Phe Thr Ile Leu Glu Gln Asn Trp Asn Gly Tyr Ala Asn Lys Lys Pro 
    130                 135                 140                 


Thr Lys Arg Val Asp Asn Tyr Tyr Gly Leu Thr His Phe Ile Glu Ile 
145                 150                 155                 160 


Pro Val Lys Ala Gly Thr Thr Val Lys Lys Glu Thr Ala Lys Lys Ser 
                165                 170                 175     


Ala 
    


<210>  3
<211>  102
<212>  PRT
<213>  Staphylococcus simulans

<400>  3

Gly Met His Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val 
1               5                   10                  15      


Lys Ala Ile Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr 
            20                  25                  30          


Gly Gly Gly Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg 
        35                  40                  45              


Gln Trp Tyr Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr 
    50                  55                  60                  


Val Lys Ala Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser 
65                  70                  75                  80  


Thr Ala Pro His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn 
                85                  90                  95      


Ser Thr Ala Gln Asp Pro 
            100         


<210>  4
<211>  149
<212>  PRT
<213>  Staphylococcus simulans

<400>  4

His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys Lys Gly Tyr Gly 
1               5                   10                  15      


Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His Tyr Gly Val 
            20                  25                  30          


Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile Ser Ser Gly 
        35                  40                  45              


Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly Asn Gln Ile 
    50                  55                  60                  


Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp Tyr Met His Leu 
65                  70                  75                  80  


Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala Gly Gln Ile 
                85                  90                  95      


Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro His Leu His 
            100                 105                 110         


Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala Gln Asp Pro 
        115                 120                 125             


Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly Gly Thr Val 
    130                 135                 140                 


Thr Pro Thr Pro Asn 
145                 


<210>  5
<211>  69
<212>  PRT
<213>  Staphylococcus capitis EPK1

<400>  5

Ser Glu Ser Ala Ser Phe Thr Ala Asn Thr Asp Ile Ile Thr Arg Leu 
1               5                   10                  15      


Thr Gly Pro Phe Arg Ser Met Pro Gln Ser Gly Val Leu Arg Lys Gly 
            20                  25                  30          


Leu Thr Ile Lys Tyr Asp Glu Val Met Lys Gln Asp Gly His Val Trp 
        35                  40                  45              


Val Gly Tyr Asn Thr Asn Ser Gly Lys Arg Val Tyr Leu Pro Val Arg 
    50                  55                  60                  


Thr Trp Asn Glu Ser 
65                  


<210>  6
<211>  94
<212>  PRT
<213>  Staphylococcus capitis EPK1

<400>  6

Asn Gly Tyr Lys Thr Asn Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser 
1               5                   10                  15      


Ala Ser Phe Thr Ala Asn Thr Asp Ile Ile Thr Arg Leu Thr Gly Pro 
            20                  25                  30          


Phe Arg Ser Met Pro Gln Ser Gly Val Leu Arg Lys Gly Leu Thr Ile 
        35                  40                  45              


Lys Tyr Asp Glu Val Met Lys Gln Asp Gly His Val Trp Val Gly Tyr 
    50                  55                  60                  


Asn Thr Asn Ser Gly Lys Arg Val Tyr Leu Pro Val Arg Thr Trp Asn 
65                  70                  75                  80  


Glu Ser Thr Gly Glu Leu Gly Pro Leu Trp Gly Thr Ile Lys 
                85                  90                  


<210>  7
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  Linker sequence

<400>  7

Gly Gly Gly Gly Ser 
1               5   


<210>  8
<211>  245
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Sequence comprising M23 endopeptidase domain of lysostaphin and 
       the cell wall binding domain of ALE-1 endolysin

<400>  8

His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys Lys Gly Tyr Gly 
1               5                   10                  15      


Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His Tyr Gly Val 
            20                  25                  30          


Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile Ser Ser Gly 
        35                  40                  45              


Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly Asn Gln Ile 
    50                  55                  60                  


Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp Tyr Met His Leu 
65                  70                  75                  80  


Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala Gly Gln Ile 
                85                  90                  95      


Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro His Leu His 
            100                 105                 110         


Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala Gln Asp Pro 
        115                 120                 125             


Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly Gly Thr Val 
    130                 135                 140                 


Thr Pro Thr Pro Asn Pro Gly Asn Gly Tyr Lys Thr Asn Lys Tyr Gly 
145                 150                 155                 160 


Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr Ala Asn Thr Asp Ile 
                165                 170                 175     


Ile Thr Arg Leu Thr Gly Pro Phe Arg Ser Met Pro Gln Ser Gly Val 
            180                 185                 190         


Leu Arg Lys Gly Leu Thr Ile Lys Tyr Asp Glu Val Met Lys Gln Asp 
        195                 200                 205             


Gly His Val Trp Val Gly Tyr Asn Thr Asn Ser Gly Lys Arg Val Tyr 
    210                 215                 220                 


Leu Pro Val Arg Thr Trp Asn Glu Ser Thr Gly Glu Leu Gly Pro Leu 
225                 230                 235                 240 


Trp Gly Thr Ile Lys 
                245 


<210>  9
<211>  424
<212>  PRT
<213>  artificial sequence

<220>
<223>  Fusion protein comprising CHAP domain of LysK endolysin, M23 
       endopeptidase domain of lysostaphin and the cell wall binding 
       domain of ALE-1 endolysin; w/o N-terminal Methionine

<400>  9

Ala Lys Thr Gln Ala Glu Ile Asn Lys Arg Leu Asp Ala Tyr Ala Lys 
1               5                   10                  15      


Gly Thr Val Asp Ser Pro Tyr Arg Val Lys Lys Ala Thr Ser Tyr Asp 
            20                  25                  30          


Pro Ser Phe Gly Val Met Glu Ala Gly Ala Ile Asp Ala Asp Gly Tyr 
        35                  40                  45              


Tyr His Ala Gln Cys Gln Asp Leu Ile Thr Asp Tyr Val Leu Trp Leu 
    50                  55                  60                  


Thr Asp Asn Lys Val Arg Thr Trp Gly Asn Ala Lys Asp Gln Ile Lys 
65                  70                  75                  80  


Gln Ser Tyr Gly Thr Gly Phe Lys Ile His Glu Asn Lys Pro Ser Thr 
                85                  90                  95      


Val Pro Lys Lys Gly Trp Ile Ala Val Phe Thr Ser Gly Ser Tyr Glu 
            100                 105                 110         


Gln Trp Gly His Ile Gly Ile Val Tyr Asp Gly Gly Asn Thr Ser Thr 
        115                 120                 125             


Phe Thr Ile Leu Glu Gln Asn Trp Asn Gly Tyr Ala Asn Lys Lys Pro 
    130                 135                 140                 


Thr Lys Arg Val Asp Asn Tyr Tyr Gly Leu Thr His Phe Ile Glu Ile 
145                 150                 155                 160 


Pro Val Lys Ala Gly Thr Thr Val Lys Lys Glu Thr Ala Lys Lys Ser 
                165                 170                 175     


Ala Thr Ser His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys Lys 
            180                 185                 190         


Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His 
        195                 200                 205             


Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile 
    210                 215                 220                 


Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly 
225                 230                 235                 240 


Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp Tyr 
                245                 250                 255     


Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala 
            260                 265                 270         


Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro 
        275                 280                 285             


His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala 
    290                 295                 300                 


Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly 
305                 310                 315                 320 


Gly Thr Val Thr Pro Thr Pro Asn Pro Gly Asn Gly Tyr Lys Thr Asn 
                325                 330                 335     


Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr Ala Asn 
            340                 345                 350         


Thr Asp Ile Ile Thr Arg Leu Thr Gly Pro Phe Arg Ser Met Pro Gln 
        355                 360                 365             


Ser Gly Val Leu Arg Lys Gly Leu Thr Ile Lys Tyr Asp Glu Val Met 
    370                 375                 380                 


Lys Gln Asp Gly His Val Trp Val Gly Tyr Asn Thr Asn Ser Gly Lys 
385                 390                 395                 400 


Arg Val Tyr Leu Pro Val Arg Thr Trp Asn Glu Ser Thr Gly Glu Leu 
                405                 410                 415     


Gly Pro Leu Trp Gly Thr Ile Lys 
            420                 


<210>  10
<211>  425
<212>  PRT
<213>  artificial sequence

<220>
<223>  Fusion protein comprising CHAP domain of LysK endolysin, M23 
       endopeptidase domain of lysostaphin and the cell wall binding 
       domain of ALE-1 endolysin

<400>  10

Met Ala Lys Thr Gln Ala Glu Ile Asn Lys Arg Leu Asp Ala Tyr Ala 
1               5                   10                  15      


Lys Gly Thr Val Asp Ser Pro Tyr Arg Val Lys Lys Ala Thr Ser Tyr 
            20                  25                  30          


Asp Pro Ser Phe Gly Val Met Glu Ala Gly Ala Ile Asp Ala Asp Gly 
        35                  40                  45              


Tyr Tyr His Ala Gln Cys Gln Asp Leu Ile Thr Asp Tyr Val Leu Trp 
    50                  55                  60                  


Leu Thr Asp Asn Lys Val Arg Thr Trp Gly Asn Ala Lys Asp Gln Ile 
65                  70                  75                  80  


Lys Gln Ser Tyr Gly Thr Gly Phe Lys Ile His Glu Asn Lys Pro Ser 
                85                  90                  95      


Thr Val Pro Lys Lys Gly Trp Ile Ala Val Phe Thr Ser Gly Ser Tyr 
            100                 105                 110         


Glu Gln Trp Gly His Ile Gly Ile Val Tyr Asp Gly Gly Asn Thr Ser 
        115                 120                 125             


Thr Phe Thr Ile Leu Glu Gln Asn Trp Asn Gly Tyr Ala Asn Lys Lys 
    130                 135                 140                 


Pro Thr Lys Arg Val Asp Asn Tyr Tyr Gly Leu Thr His Phe Ile Glu 
145                 150                 155                 160 


Ile Pro Val Lys Ala Gly Thr Thr Val Lys Lys Glu Thr Ala Lys Lys 
                165                 170                 175     


Ser Ala Thr Ser His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys 
            180                 185                 190         


Lys Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met 
        195                 200                 205             


His Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala 
    210                 215                 220                 


Ile Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly 
225                 230                 235                 240 


Gly Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp 
                245                 250                 255     


Tyr Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys 
            260                 265                 270         


Ala Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala 
        275                 280                 285             


Pro His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr 
    290                 295                 300                 


Ala Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala 
305                 310                 315                 320 


Gly Gly Thr Val Thr Pro Thr Pro Asn Pro Gly Asn Gly Tyr Lys Thr 
                325                 330                 335     


Asn Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr Ala 
            340                 345                 350         


Asn Thr Asp Ile Ile Thr Arg Leu Thr Gly Pro Phe Arg Ser Met Pro 
        355                 360                 365             


Gln Ser Gly Val Leu Arg Lys Gly Leu Thr Ile Lys Tyr Asp Glu Val 
    370                 375                 380                 


Met Lys Gln Asp Gly His Val Trp Val Gly Tyr Asn Thr Asn Ser Gly 
385                 390                 395                 400 


Lys Arg Val Tyr Leu Pro Val Arg Thr Trp Asn Glu Ser Thr Gly Glu 
                405                 410                 415     


Leu Gly Pro Leu Trp Gly Thr Ile Lys 
            420                 425 


<210>  11
<211>  6
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  11

Lys Arg Lys Lys Arg Lys 
1               5       


<210>  12
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  synethtic sequence


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  12

Lys Arg Xaa Lys Arg 
1               5   


<210>  13
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  13

Lys Arg Ser Lys Arg 
1               5   


<210>  14
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  14

Lys Arg Gly Ser Gly 
1               5   


<210>  15
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  15

Lys Arg Lys Lys Arg Lys Lys Arg Lys 
1               5                   


<210>  16
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  16

Arg Arg Arg Arg Arg Arg Arg Arg Arg 
1               5                   


<210>  17
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  17

Lys Lys Lys Lys Lys Lys Lys Lys 
1               5               


<210>  18
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  18

Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys 
1               5                   10  


<210>  19
<211>  12
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  19

Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys 
1               5                   10          


<210>  20
<211>  14
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  20

Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg 
1               5                   10                  


<210>  21
<211>  16
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  21

Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys 
1               5                   10                  15      


<210>  22
<211>  18
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  22

Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys 
1               5                   10                  15      


Arg Lys 
        


<210>  23
<211>  19
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  23

Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys 
1               5                   10                  15      


Arg Lys Lys 
            


<210>  24
<211>  19
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  24

Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg 
1               5                   10                  15      


Arg Arg Arg 
            


<210>  25
<211>  19
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  25

Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys 
1               5                   10                  15      


Lys Lys Lys 
            


<210>  26
<211>  20
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  26

Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Ser Lys Arg Lys Lys Arg 
1               5                   10                  15      


Lys Lys Arg Lys 
            20  


<210>  27
<211>  21
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  27

Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Ser Lys Arg Lys Lys Arg 
1               5                   10                  15      


Lys Lys Arg Lys Lys 
            20      


<210>  28
<211>  21
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  28

Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys 
1               5                   10                  15      


Arg Lys Lys Arg Lys 
            20      


<210>  29
<211>  22
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  29

Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Gly Ser Gly Lys Arg Lys 
1               5                   10                  15      


Lys Arg Lys Lys Arg Lys 
            20          


<210>  30
<211>  24
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  30

Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Gly Ser Gly Ser Gly Lys 
1               5                   10                  15      


Arg Lys Lys Arg Lys Lys Arg Lys 
            20                  


<210>  31
<211>  25
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  31

Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys 
1               5                   10                  15      


Arg Lys Lys Arg Lys Lys Arg Lys Lys 
            20                  25  


<210>  32
<211>  31
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  32

Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Ser Lys Arg Lys Lys Arg 
1               5                   10                  15      


Lys Lys Arg Lys Arg Ser Lys Arg Lys Lys Arg Lys Lys Arg Lys 
            20                  25                  30      


<210>  33
<211>  38
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  33

Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Gly Ser Gly Ser Gly Lys 
1               5                   10                  15      


Arg Lys Lys Arg Lys Lys Arg Lys Gly Ser Gly Ser Gly Lys Arg Lys 
            20                  25                  30          


Lys Arg Lys Lys Arg Lys 
        35              


<210>  34
<211>  39
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  34

Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys 
1               5                   10                  15      


Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg 
            20                  25                  30          


Lys Lys Arg Lys Lys Arg Lys 
        35                  


<210>  35
<211>  42
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  35

Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Ser Lys Arg Lys Lys Arg 
1               5                   10                  15      


Lys Lys Arg Lys Arg Ser Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg 
            20                  25                  30          


Ser Lys Arg Lys Lys Arg Lys Lys Arg Lys 
        35                  40          


<210>  36
<211>  3
<212>  PRT
<213>  Artificial sequence

<220>
<223>  cationic peptide KNA

<400>  36

Lys Asn Ala 
1           


<210>  37
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  cationic peptide GGSKNA

<400>  37

Gly Gly Ser Lys Asn Ala 
1               5       


<210>  38
<211>  3
<212>  PRT
<213>  Artificial sequence

<220>
<223>  cationic peptide KNK

<400>  38

Lys Asn Lys 
1           


<210>  39
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  cationic peptide GGSKNK

<400>  39

Gly Gly Ser Lys Asn Lys 
1               5       


<210>  40
<211>  37
<212>  PRT
<213>  Homo sapiens

<400>  40

Leu Leu Gly Asp Phe Phe Arg Lys Ser Lys Glu Lys Ile Gly Lys Glu 
1               5                   10                  15      


Phe Lys Arg Ile Val Gln Arg Ile Lys Asp Phe Leu Arg Asn Leu Val 
            20                  25                  30          


Pro Arg Thr Glu Ser 
        35          


<210>  41
<211>  29
<212>  PRT
<213>  unknown

<220>
<223>  SMAP-29 sheep

<400>  41

Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys Lys 
1               5                   10                  15      


Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly 
            20                  25                  


<210>  42
<211>  13
<212>  PRT
<213>  unknown

<220>
<223>  Indolicidine bovine

<400>  42

Ile Leu Pro Trp Lys Trp Pro Trp Trp Pro Trp Arg Arg 
1               5                   10              


<210>  43
<211>  18
<212>  PRT
<213>  unknown

<220>
<223>  Protegrin Porcine

<400>  43

Arg Gly Gly Arg Leu Cys Tyr Cys Arg Arg Arg Phe Cys Val Cys Val 
1               5                   10                  15      


Gly Arg 
        


<210>  44
<211>  31
<212>  PRT
<213>  unknown

<220>
<223>  Cecropin P1 Mammal (pig)

<400>  44

Ser Trp Leu Ser Lys Thr Ala Lys Lys Leu Glu Asn Ser Ala Lys Lys 
1               5                   10                  15      


Arg Ile Ser Glu Gly Ile Ala Ile Ala Ile Gln Gly Gly Pro Arg 
            20                  25                  30      


<210>  45
<211>  23
<212>  PRT
<213>  unknown

<220>
<223>  Magainin frog

<400>  45

Gly Ile Gly Lys Phe Leu His Ser Ala Lys Lys Phe Gly Lys Ala Phe 
1               5                   10                  15      


Val Gly Glu Ile Met Asn Ser 
            20              


<210>  46
<211>  25
<212>  PRT
<213>  unknown

<220>
<223>  Pleurocidin fish

<400>  46

Gly Trp Gly Ser Phe Phe Lys Lys Ala Ala His Val Gly Lys His Val 
1               5                   10                  15      


Gly Lys Ala Ala Leu Thr His Tyr Leu 
            20                  25  


<210>  47
<211>  36
<212>  PRT
<213>  Aedes aegypti

<400>  47

Gly Gly Leu Lys Lys Leu Gly Lys Lys Leu Glu Gly Ala Gly Lys Arg 
1               5                   10                  15      


Val Phe Asn Ala Ala Glu Lys Ala Leu Pro Val Val Ala Gly Ala Lys 
            20                  25                  30          


Ala Leu Arg Lys 
        35      


<210>  48
<211>  40
<212>  PRT
<213>  Drosophila melanogaster

<400>  48

Gly Trp Leu Lys Lys Ile Gly Lys Lys Ile Glu Arg Val Gly Gln His 
1               5                   10                  15      


Thr Arg Asp Ala Thr Ile Gln Gly Leu Gly Ile Pro Gln Gln Ala Ala 
            20                  25                  30          


Asn Val Ala Ala Thr Ala Arg Gly 
        35                  40  


<210>  49
<211>  21
<212>  PRT
<213>  unknown

<220>
<223>  Buforin II vertebrate

<400>  49

Thr Arg Ser Ser Arg Ala Gly Leu Gln Phe Pro Val Gly Arg Val His 
1               5                   10                  15      


Arg Leu Leu Arg Lys 
            20      


<210>  50
<211>  39
<212>  PRT
<213>  unknown

<220>
<223>  Sarcotoxin IA Fly

<400>  50

Gly Trp Leu Lys Lys Ile Gly Lys Lys Ile Glu Arg Val Gly Gln His 
1               5                   10                  15      


Thr Arg Asp Ala Thr Ile Gln Gly Leu Gly Ile Ala Gln Gln Ala Ala 
            20                  25                  30          


Asn Val Ala Ala Thr Ala Arg 
        35                  


<210>  51
<211>  17
<212>  PRT
<213>  Apis mellifera

<400>  51

Ala Asn Arg Pro Val Tyr Ile Pro Pro Pro Arg Pro Pro His Pro Arg 
1               5                   10                  15      


Leu 
    


<210>  52
<211>  24
<212>  PRT
<213>  unknown

<220>
<223>  Ascaphine 5 Frog

<400>  52

Gly Ile Lys Asp Trp Ile Lys Gly Ala Ala Lys Lys Leu Ile Lys Thr 
1               5                   10                  15      


Val Ala Ser His Ile Ala Asn Gln 
            20                  


<210>  53
<211>  22
<212>  PRT
<213>  unknown

<220>
<223>  Nigrocine 2 Frog

<400>  53

Gly Leu Leu Ser Lys Val Leu Gly Val Gly Lys Lys Val Leu Cys Gly 
1               5                   10                  15      


Val Ser Gly Leu Val Cys 
            20          


<210>  54
<211>  24
<212>  PRT
<213>  unknown

<220>
<223>  Pseudin 1 Rana Frog

<400>  54

Gly Leu Asn Thr Leu Lys Lys Val Phe Gln Gly Leu His Glu Ala Ile 
1               5                   10                  15      


Lys Leu Ile Asn Asn His Val Gln 
            20                  


<210>  55
<211>  18
<212>  PRT
<213>  unknown

<220>
<223>  Ranalexin Frog

<400>  55

Phe Leu Gly Gly Leu Ile Val Pro Ala Met Ile Cys Ala Val Thr Lys 
1               5                   10                  15      


Lys Cys 
        


<210>  56
<211>  26
<212>  PRT
<213>  unknown

<220>
<223>  Melittin bee

<400>  56

Gly Ile Gly Ala Val Leu Lys Val Leu Thr Thr Gly Leu Pro Ala Leu 
1               5                   10                  15      


Ile Ser Trp Ile Lys Arg Lys Arg Gln Gln 
            20                  25      


<210>  57
<211>  25
<212>  PRT
<213>  unknown

<220>
<223>  Lycotoxin 1 Spider

<400>  57

Ile Trp Leu Thr Ala Leu Lys Phe Leu Gly Lys His Ala Ala Lys Lys 
1               5                   10                  15      


Leu Ala Lys Gln Gln Leu Ser Lys Leu 
            20                  25  


<210>  58
<211>  19
<212>  PRT
<213>  unknown

<220>
<223>  Parasin 1 Fish

<400>  58

Lys Gly Arg Gly Lys Gln Gly Gly Lys Val Arg Ala Lys Ala Lys Thr 
1               5                   10                  15      


Arg Ser Ser 
            


<210>  59
<211>  39
<212>  PRT
<213>  unknown

<220>
<223>  Buforin I Toad

<400>  59

Ala Gly Arg Gly Lys Gln Gly Gly Lys Val Arg Ala Lys Ala Lys Thr 
1               5                   10                  15      


Arg Ser Ser Arg Ala Gly Leu Gln Phe Pro Val Gly Arg Val His Arg 
            20                  25                  30          


Leu Leu Arg Lys Gly Asn Tyr 
        35                  


<210>  60
<211>  34
<212>  PRT
<213>  unknown

<220>
<223>  Dermaseptin 1 Frog

<400>  60

Ala Leu Trp Lys Thr Met Leu Lys Lys Leu Gly Thr Met Ala Leu His 
1               5                   10                  15      


Ala Gly Lys Ala Ala Leu Gly Ala Ala Ala Asp Thr Ile Ser Gln Gly 
            20                  25                  30          


Thr Gln 
        


<210>  61
<211>  12
<212>  PRT
<213>  unknown

<220>
<223>  Bactenecin 1 Cow

<400>  61

Arg Leu Cys Arg Ile Val Val Ile Arg Val Cys Arg 
1               5                   10          


<210>  62
<211>  21
<212>  PRT
<213>  unknown

<220>
<223>  Thanatin Insect

<400>  62

Gly Ser Lys Lys Pro Val Pro Ile Ile Tyr Cys Asn Arg Arg Thr Gly 
1               5                   10                  15      


Lys Cys Gln Arg Met 
            20      


<210>  63
<211>  19
<212>  PRT
<213>  unknown

<220>
<223>  Brevinin 1T Rana frogs

<400>  63

Val Asn Pro Ile Ile Leu Gly Val Leu Pro Lys Val Cys Leu Ile Thr 
1               5                   10                  15      


Lys Lys Cys 
            


<210>  64
<211>  26
<212>  PRT
<213>  unknown

<220>
<223>  Ranateurin 1 Rana frog

<400>  64

Ser Met Leu Ser Val Leu Lys Asn Leu Gly Lys Val Gly Leu Gly Phe 
1               5                   10                  15      


Val Ala Cys Lys Ile Asn Ile Lys Gln Cys 
            20                  25      


<210>  65
<211>  46
<212>  PRT
<213>  unknown

<220>
<223>  Esculentin 1 Rana frogs

<400>  65

Gly Ile Phe Ser Lys Leu Gly Arg Lys Lys Ile Lys Asn Leu Leu Ile 
1               5                   10                  15      


Ser Gly Leu Lys Asn Val Gly Lys Glu Val Gly Met Asp Val Val Arg 
            20                  25                  30          


Thr Gly Ile Lys Ile Ala Gly Cys Lys Ile Lys Gly Glu Cys 
        35                  40                  45      


<210>  66
<211>  17
<212>  PRT
<213>  Limulus polyphemus

<400>  66

Arg Trp Cys Phe Arg Val Cys Tyr Arg Gly Ile Cys Tyr Arg Lys Cys 
1               5                   10                  15      


Arg 
    


<210>  67
<211>  25
<212>  PRT
<213>  unknown

<220>
<223>  Androctonin Scorpion

<400>  67

Arg Ser Val Cys Arg Gln Ile Lys Ile Cys Arg Arg Arg Gly Gly Cys 
1               5                   10                  15      


Tyr Tyr Lys Cys Thr Asn Arg Pro Tyr 
            20                  25  


<210>  68
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  68

Asp Cys Tyr Cys Arg Ile Pro Ala Cys Ile Ala Gly Glu Arg Arg Tyr 
1               5                   10                  15      


Gly Thr Cys Ile Tyr Gln Gly Arg Leu Trp Ala Phe Cys Cys 
            20                  25                  30  


<210>  69
<211>  38
<212>  PRT
<213>  unknown

<220>
<223>  beta-defensin cow

<400>  69

Asn Pro Val Ser Cys Val Arg Asn Lys Gly Ile Cys Val Pro Ile Arg 
1               5                   10                  15      


Cys Pro Gly Ser Met Lys Gln Ile Gly Thr Cys Val Gly Arg Ala Val 
            20                  25                  30          


Lys Cys Cys Arg Lys Lys 
        35              


<210>  70
<211>  18
<212>  PRT
<213>  unknown

<220>
<223>  theta-defensin monkey

<400>  70

Gly Phe Cys Arg Cys Leu Cys Arg Arg Gly Val Cys Arg Cys Ile Cys 
1               5                   10                  15      


Thr Arg 
        


<210>  71
<211>  40
<212>  PRT
<213>  unknown

<220>
<223>  defensin (sapecin A) insect

<400>  71

Ala Thr Cys Asp Leu Leu Ser Gly Thr Gly Ile Asn His Ser Ala Cys 
1               5                   10                  15      


Ala Ala His Cys Leu Leu Arg Gly Asn Arg Gly Gly Tyr Cys Asn Gly 
            20                  25                  30          


Lys Ala Val Cys Val Cys Arg Asn 
        35                  40  


<210>  72
<211>  46
<212>  PRT
<213>  unknown

<220>
<223>  Thionin (crambin) plant

<400>  72

Thr Thr Cys Cys Pro Ser Ile Val Ala Arg Ser Asn Phe Asn Val Cys 
1               5                   10                  15      


Arg Ile Pro Gly Thr Pro Glu Ala Ile Cys Ala Thr Tyr Thr Gly Cys 
            20                  25                  30          


Ile Ile Ile Pro Gly Ala Thr Cys Pro Gly Asp Tyr Ala Asn 
        35                  40                  45      


<210>  73
<211>  50
<212>  PRT
<213>  unknown

<220>
<223>  defensin from radish

<400>  73

Gln Lys Leu Cys Gln Arg Pro Ser Gly Thr Trp Ser Gly Val Cys Gly 
1               5                   10                  15      


Asn Asn Asn Ala Cys Lys Asn Gln Cys Ile Arg Leu Glu Lys Ala Arg 
            20                  25                  30          


His Gly Ser Cys Asn Tyr Val Phe Pro Ala His Cys Ile Cys Tyr Phe 
        35                  40                  45              


Pro Cys 
    50  


<210>  74
<211>  44
<212>  PRT
<213>  Drosophila melanogaster

<400>  74

Asp Cys Leu Ser Gly Arg Tyr Lys Gly Pro Cys Ala Val Trp Asp Asn 
1               5                   10                  15      


Glu Thr Cys Arg Arg Val Cys Lys Glu Glu Gly Arg Ser Ser Gly His 
            20                  25                  30          


Cys Ser Pro Ser Leu Lys Cys Trp Cys Glu Gly Cys 
        35                  40                  


<210>  75
<211>  25
<212>  PRT
<213>  Homo sapiens

<400>  75

Asp Thr His Phe Pro Ile Cys Ile Phe Cys Cys Gly Cys Cys His Arg 
1               5                   10                  15      


Ser Lys Cys Gly Met Cys Cys Lys Thr 
            20                  25  


<210>  76
<211>  44
<212>  PRT
<213>  unknown

<220>
<223>  Bac 5 Cow

<400>  76

Arg Phe Arg Pro Pro Ile Arg Arg Pro Pro Ile Arg Pro Pro Phe Tyr 
1               5                   10                  15      


Pro Pro Phe Arg Pro Pro Ile Arg Pro Pro Ile Phe Pro Pro Ile Arg 
            20                  25                  30          


Pro Pro Phe Arg Pro Pro Leu Gly Arg Pro Phe Pro 
        35                  40                  


<210>  77
<211>  39
<212>  PRT
<213>  unknown

<220>
<223>  PR-39 Pig

<400>  77

Arg Arg Arg Pro Arg Pro Pro Tyr Leu Pro Arg Pro Arg Pro Pro Pro 
1               5                   10                  15      


Phe Phe Pro Pro Arg Leu Pro Pro Arg Ile Pro Pro Gly Phe Pro Pro 
            20                  25                  30          


Arg Phe Pro Pro Arg Phe Pro 
        35                  


<210>  78
<211>  20
<212>  PRT
<213>  unknown

<220>
<223>  Pyrrhocoricin Insect

<400>  78

Val Asp Lys Gly Ser Tyr Leu Pro Arg Pro Thr Pro Pro Arg Pro Ile 
1               5                   10                  15      


Tyr Asn Arg Asn 
            20  


<210>  79
<211>  24
<212>  PRT
<213>  Homo sapiens

<400>  79

Asp Ser His Ala Lys Arg His His Gly Tyr Lys Arg Lys Phe His Glu 
1               5                   10                  15      


Lys His His Ser His Arg Gly Tyr 
            20                  


<210>  80
<211>  19
<212>  PRT
<213>  Unknown

<220>
<223>  ECP19

<400>  80

Arg Pro Pro Gln Phe Thr Arg Ala Gln Trp Phe Ala Ile Gln His Ile 
1               5                   10                  15      


Ser Leu Asn 
            


<210>  81
<211>  23
<212>  PRT
<213>  Unknown

<220>
<223>  MSI-594

<400>  81

Gly Ile Gly Lys Phe Leu Lys Lys Ala Lys Lys Gly Ile Gly Ala Val 
1               5                   10                  15      


Leu Lys Val Leu Thr Thr Gly 
            20              


<210>  82
<211>  35
<212>  PRT
<213>  Unknown

<220>
<223>  TL-ColM

<400>  82

Met Glu Thr Leu Thr Val His Ala Pro Ser Pro Ser Thr Asn Leu Pro 
1               5                   10                  15      


Ser Tyr Gly Asn Gly Ala Phe Ser Leu Ser Ala Pro His Val Pro Gly 
            20                  25                  30          


Ala Gly Pro 
        35  


<210>  83
<211>  18
<212>  PRT
<213>  Unknown

<220>
<223>  SBO

<400>  83

Lys Leu Lys Lys Ile Ala Gln Lys Ile Lys Asn Phe Phe Ala Lys Leu 
1               5                   10                  15      


Val Ala 
        


<210>  84
<211>  26
<212>  PRT
<213>  unknown

<220>
<223>  Macedocin

<400>  84

Gly Lys Asn Gly Val Phe Lys Thr Ile Ser His Glu Cys His Leu Asn 
1               5                   10                  15      


Thr Trp Ala Phe Leu Ala Thr Cys Cys Ser 
            20                  25      


<210>  85
<211>  22
<212>  PRT
<213>  unknown

<220>
<223>  Macedocin (Trunc)

<400>  85

Gly Lys Asn Gly Val Phe Lys Thr Ile Ser His Glu Cys His Leu Asn 
1               5                   10                  15      


Thr Trp Ala Phe Leu Ala 
            20          


<210>  86
<211>  28
<212>  PRT
<213>  unknown

<220>
<223>  D16

<400>  86

Ala Cys Lys Leu Lys Ser Leu Leu Lys Thr Leu Ser Lys Ala Lys Lys 
1               5                   10                  15      


Lys Lys Leu Lys Thr Leu Leu Lys Ala Leu Ser Lys 
            20                  25              


<210>  87
<211>  17
<212>  PRT
<213>  unknown

<220>
<223>  CPF-C1

<400>  87

Gly Phe Gly Ser Leu Leu Gly Lys Ala Leu Arg Leu Gly Ala Asn Val 
1               5                   10                  15      


Leu 
    


<210>  88
<211>  34
<212>  PRT
<213>  unknown

<220>
<223>  TL-ColM

<400>  88

Glu Thr Leu Thr Val His Ala Pro Ser Pro Ser Thr Asn Leu Pro Ser 
1               5                   10                  15      


Tyr Gly Asn Gly Ala Phe Ser Leu Ser Ala Pro His Val Pro Gly Ala 
            20                  25                  30          


Gly Pro 
        


<210>  89
<211>  26
<212>  PRT
<213>  unknown

<220>
<223>  TM-174E

<400>  89

Leu Ile Ser Lys Gly Trp Pro Tyr Leu Leu Val Val Val Leu Gly Ala 
1               5                   10                  15      


Thr Ile Tyr Phe Trp Gly Asn Ser Asn Gly 
            20                  25      


<210>  90
<211>  45
<212>  PRT
<213>  unknown

<220>
<223>  ECP45

<400>  90

Arg Pro Pro Gln Phe Thr Arg Ala Gln Trp Phe Ala Ile Gln His Ile 
1               5                   10                  15      


Ser Leu Asn Pro Pro Arg Cys Thr Ile Ala Met Arg Ala Ile Asn Asn 
            20                  25                  30          


Tyr Arg Trp Arg Cys Lys Asn Gln Asn Thr Phe Leu Arg 
        35                  40                  45  


<210>  91
<211>  50
<212>  PRT
<213>  unknown

<220>
<223>  ColicinE3_1-51 (S37F)

<400>  91

Ser Gly Gly Asp Gly Arg Gly His Asn Thr Gly Ala His Ser Thr Ser 
1               5                   10                  15      


Gly Asn Ile Asn Gly Gly Pro Thr Gly Leu Gly Val Gly Gly Gly Ala 
            20                  25                  30          


Ser Asp Gly Phe Gly Trp Ser Ser Glu Asn Asn Pro Trp Gly Gly Gly 
        35                  40                  45              


Ser Gly 
    50  


<210>  92
<211>  68
<212>  PRT
<213>  unknown

<220>
<223>  ColicinE3_1-69 (S37F)

<400>  92

Ser Gly Gly Asp Gly Arg Gly His Asn Thr Gly Ala His Ser Thr Ser 
1               5                   10                  15      


Gly Asn Ile Asn Gly Gly Pro Thr Gly Leu Gly Val Gly Gly Gly Ala 
            20                  25                  30          


Ser Asp Gly Phe Gly Trp Ser Ser Glu Asn Asn Pro Trp Gly Gly Gly 
        35                  40                  45              


Ser Gly Ser Gly Ile His Trp Gly Gly Gly Ser Gly His Gly Asn Gly 
    50                  55                  60                  


Gly Gly Asn Gly 
65              


<210>  93
<211>  52
<212>  PRT
<213>  unknown

<220>
<223>  ColicinD_1-53

<400>  93

Ser Asp Tyr Glu Gly Ser Gly Pro Thr Glu Gly Ile Asp Tyr Gly His 
1               5                   10                  15      


Ser Met Val Val Trp Pro Ser Thr Gly Leu Ile Ser Gly Gly Asp Val 
            20                  25                  30          


Lys Pro Gly Gly Ser Ser Gly Ile Ala Pro Ser Met Pro Pro Gly Trp 
        35                  40                  45              


Gly Asp Tyr Ser 
    50          


<210>  94
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  94

His Pro Gln Tyr Asn Gln Arg 
1               5           


<210>  95
<211>  34
<212>  PRT
<213>  Limulus polyphemus

<400>  95

Gly Phe Lys Leu Lys Gly Met Ala Arg Ile Ser Cys Leu Pro Asn Gly 
1               5                   10                  15      


Gln Trp Ser Asn Phe Pro Pro Lys Cys Ile Arg Glu Cys Ala Met Val 
            20                  25                  30          


Ser Ser 
        


<210>  96
<211>  18
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  96

Gly Phe Phe Ile Pro Ala Val Ile Leu Pro Ser Ile Ala Phe Leu Ile 
1               5                   10                  15      


Val Pro 
        


<210>  97
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  97

Phe Phe Val Ala Pro 
1               5   


<210>  98
<211>  13
<212>  PRT
<213>  unknown

<220>
<223>  alpha4-helix of T4 lysozyme

<400>  98

Pro Asn Arg Ala Lys Arg Val Ile Thr Thr Phe Arg Thr 
1               5                   10              


<210>  99
<211>  27
<212>  PRT
<213>  artificial

<220>
<223>  synthetic sequence

<400>  99

Lys Arg Trp Val Lys Arg Val Lys Arg Val Lys Arg Trp Val Lys Arg 
1               5                   10                  15      


Val Val Arg Val Val Lys Arg Trp Val Lys Arg 
            20                  25          


<210>  100
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic sequence; MW2

<400>  100

Gly Lys Pro Gly Trp Leu Ile Lys Val Ala Leu Lys Phe Lys Lys Leu 
1               5                   10                  15      


Ile Arg Arg Pro Leu Lys Arg Leu Ala 
            20                  25  


<210>  101
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  His-Tag (6x)

<400>  101

His His His His His His 
1               5       


<210>  102
<211>  430
<212>  PRT
<213>  artificial sequence

<220>
<223>  Fusion protein comprising CHAP domain of LysK endolysin, M23 
       endopeptidase domain of lysostaphin, cell wall binding domain of 
       ALE-1, and His6-tag; w/o N-terminal methionine

<400>  102

Ala Lys Thr Gln Ala Glu Ile Asn Lys Arg Leu Asp Ala Tyr Ala Lys 
1               5                   10                  15      


Gly Thr Val Asp Ser Pro Tyr Arg Val Lys Lys Ala Thr Ser Tyr Asp 
            20                  25                  30          


Pro Ser Phe Gly Val Met Glu Ala Gly Ala Ile Asp Ala Asp Gly Tyr 
        35                  40                  45              


Tyr His Ala Gln Cys Gln Asp Leu Ile Thr Asp Tyr Val Leu Trp Leu 
    50                  55                  60                  


Thr Asp Asn Lys Val Arg Thr Trp Gly Asn Ala Lys Asp Gln Ile Lys 
65                  70                  75                  80  


Gln Ser Tyr Gly Thr Gly Phe Lys Ile His Glu Asn Lys Pro Ser Thr 
                85                  90                  95      


Val Pro Lys Lys Gly Trp Ile Ala Val Phe Thr Ser Gly Ser Tyr Glu 
            100                 105                 110         


Gln Trp Gly His Ile Gly Ile Val Tyr Asp Gly Gly Asn Thr Ser Thr 
        115                 120                 125             


Phe Thr Ile Leu Glu Gln Asn Trp Asn Gly Tyr Ala Asn Lys Lys Pro 
    130                 135                 140                 


Thr Lys Arg Val Asp Asn Tyr Tyr Gly Leu Thr His Phe Ile Glu Ile 
145                 150                 155                 160 


Pro Val Lys Ala Gly Thr Thr Val Lys Lys Glu Thr Ala Lys Lys Ser 
                165                 170                 175     


Ala Thr Ser His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys Lys 
            180                 185                 190         


Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His 
        195                 200                 205             


Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile 
    210                 215                 220                 


Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly 
225                 230                 235                 240 


Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp Tyr 
                245                 250                 255     


Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala 
            260                 265                 270         


Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro 
        275                 280                 285             


His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala 
    290                 295                 300                 


Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly 
305                 310                 315                 320 


Gly Thr Val Thr Pro Thr Pro Asn Pro Gly Asn Gly Tyr Lys Thr Asn 
                325                 330                 335     


Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr Ala Asn 
            340                 345                 350         


Thr Asp Ile Ile Thr Arg Leu Thr Gly Pro Phe Arg Ser Met Pro Gln 
        355                 360                 365             


Ser Gly Val Leu Arg Lys Gly Leu Thr Ile Lys Tyr Asp Glu Val Met 
    370                 375                 380                 


Lys Gln Asp Gly His Val Trp Val Gly Tyr Asn Thr Asn Ser Gly Lys 
385                 390                 395                 400 


Arg Val Tyr Leu Pro Val Arg Thr Trp Asn Glu Ser Thr Gly Glu Leu 
                405                 410                 415     


Gly Pro Leu Trp Gly Thr Ile Lys His His His His His His 
            420                 425                 430 


<210>  103
<211>  432
<212>  PRT
<213>  artificial sequence

<220>
<223>  Fusion protein comprising CHAP domain of LysK endolysin, M23 
       endopeptidase domain of lysostaphin, cell wall binding domain of 
       ALE-1, and His6-tag via GS linker; w/o N-terminal methionine

<400>  103

Ala Lys Thr Gln Ala Glu Ile Asn Lys Arg Leu Asp Ala Tyr Ala Lys 
1               5                   10                  15      


Gly Thr Val Asp Ser Pro Tyr Arg Val Lys Lys Ala Thr Ser Tyr Asp 
            20                  25                  30          


Pro Ser Phe Gly Val Met Glu Ala Gly Ala Ile Asp Ala Asp Gly Tyr 
        35                  40                  45              


Tyr His Ala Gln Cys Gln Asp Leu Ile Thr Asp Tyr Val Leu Trp Leu 
    50                  55                  60                  


Thr Asp Asn Lys Val Arg Thr Trp Gly Asn Ala Lys Asp Gln Ile Lys 
65                  70                  75                  80  


Gln Ser Tyr Gly Thr Gly Phe Lys Ile His Glu Asn Lys Pro Ser Thr 
                85                  90                  95      


Val Pro Lys Lys Gly Trp Ile Ala Val Phe Thr Ser Gly Ser Tyr Glu 
            100                 105                 110         


Gln Trp Gly His Ile Gly Ile Val Tyr Asp Gly Gly Asn Thr Ser Thr 
        115                 120                 125             


Phe Thr Ile Leu Glu Gln Asn Trp Asn Gly Tyr Ala Asn Lys Lys Pro 
    130                 135                 140                 


Thr Lys Arg Val Asp Asn Tyr Tyr Gly Leu Thr His Phe Ile Glu Ile 
145                 150                 155                 160 


Pro Val Lys Ala Gly Thr Thr Val Lys Lys Glu Thr Ala Lys Lys Ser 
                165                 170                 175     


Ala Thr Ser His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys Lys 
            180                 185                 190         


Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His 
        195                 200                 205             


Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile 
    210                 215                 220                 


Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly 
225                 230                 235                 240 


Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp Tyr 
                245                 250                 255     


Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala 
            260                 265                 270         


Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro 
        275                 280                 285             


His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala 
    290                 295                 300                 


Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly 
305                 310                 315                 320 


Gly Thr Val Thr Pro Thr Pro Asn Pro Gly Asn Gly Tyr Lys Thr Asn 
                325                 330                 335     


Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr Ala Asn 
            340                 345                 350         


Thr Asp Ile Ile Thr Arg Leu Thr Gly Pro Phe Arg Ser Met Pro Gln 
        355                 360                 365             


Ser Gly Val Leu Arg Lys Gly Leu Thr Ile Lys Tyr Asp Glu Val Met 
    370                 375                 380                 


Lys Gln Asp Gly His Val Trp Val Gly Tyr Asn Thr Asn Ser Gly Lys 
385                 390                 395                 400 


Arg Val Tyr Leu Pro Val Arg Thr Trp Asn Glu Ser Thr Gly Glu Leu 
                405                 410                 415     


Gly Pro Leu Trp Gly Thr Ile Lys Gly Ser His His His His His His 
            420                 425                 430         


<210>  104
<211>  439
<212>  PRT
<213>  artificial sequence

<220>
<223>  Fusion protein comprising CHAP domain of LysK endolysin, M23 
       endopeptidase domain of lysostaphin, cell wall binding domain of 
       ALE-1, His6-tag and synthetic peptide HPQYNQR; w/o N-terminal 
       methionine

<400>  104

Ala Lys Thr Gln Ala Glu Ile Asn Lys Arg Leu Asp Ala Tyr Ala Lys 
1               5                   10                  15      


Gly Thr Val Asp Ser Pro Tyr Arg Val Lys Lys Ala Thr Ser Tyr Asp 
            20                  25                  30          


Pro Ser Phe Gly Val Met Glu Ala Gly Ala Ile Asp Ala Asp Gly Tyr 
        35                  40                  45              


Tyr His Ala Gln Cys Gln Asp Leu Ile Thr Asp Tyr Val Leu Trp Leu 
    50                  55                  60                  


Thr Asp Asn Lys Val Arg Thr Trp Gly Asn Ala Lys Asp Gln Ile Lys 
65                  70                  75                  80  


Gln Ser Tyr Gly Thr Gly Phe Lys Ile His Glu Asn Lys Pro Ser Thr 
                85                  90                  95      


Val Pro Lys Lys Gly Trp Ile Ala Val Phe Thr Ser Gly Ser Tyr Glu 
            100                 105                 110         


Gln Trp Gly His Ile Gly Ile Val Tyr Asp Gly Gly Asn Thr Ser Thr 
        115                 120                 125             


Phe Thr Ile Leu Glu Gln Asn Trp Asn Gly Tyr Ala Asn Lys Lys Pro 
    130                 135                 140                 


Thr Lys Arg Val Asp Asn Tyr Tyr Gly Leu Thr His Phe Ile Glu Ile 
145                 150                 155                 160 


Pro Val Lys Ala Gly Thr Thr Val Lys Lys Glu Thr Ala Lys Lys Ser 
                165                 170                 175     


Ala Thr Ser His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys Lys 
            180                 185                 190         


Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His 
        195                 200                 205             


Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile 
    210                 215                 220                 


Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly 
225                 230                 235                 240 


Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp Tyr 
                245                 250                 255     


Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala 
            260                 265                 270         


Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro 
        275                 280                 285             


His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala 
    290                 295                 300                 


Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly 
305                 310                 315                 320 


Gly Thr Val Thr Pro Thr Pro Asn Pro Gly Asn Gly Tyr Lys Thr Asn 
                325                 330                 335     


Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr Ala Asn 
            340                 345                 350         


Thr Asp Ile Ile Thr Arg Leu Thr Gly Pro Phe Arg Ser Met Pro Gln 
        355                 360                 365             


Ser Gly Val Leu Arg Lys Gly Leu Thr Ile Lys Tyr Asp Glu Val Met 
    370                 375                 380                 


Lys Gln Asp Gly His Val Trp Val Gly Tyr Asn Thr Asn Ser Gly Lys 
385                 390                 395                 400 


Arg Val Tyr Leu Pro Val Arg Thr Trp Asn Glu Ser Thr Gly Glu Leu 
                405                 410                 415     


Gly Pro Leu Trp Gly Thr Ile Lys His His His His His His Leu Lys 
            420                 425                 430         


His Pro Gln Tyr Asn Gln Arg 
        435                 


<210>  105
<211>  440
<212>  PRT
<213>  artificial sequence

<220>
<223>  Fusion protein comprising CHAP domain of LysK endolysin, M23 
       endopeptidase domain of lysostaphin, cell wall binding domain of 
       ALE-1, His6-tag and synthetic peptide HPQYNQR

<400>  105

Met Ala Lys Thr Gln Ala Glu Ile Asn Lys Arg Leu Asp Ala Tyr Ala 
1               5                   10                  15      


Lys Gly Thr Val Asp Ser Pro Tyr Arg Val Lys Lys Ala Thr Ser Tyr 
            20                  25                  30          


Asp Pro Ser Phe Gly Val Met Glu Ala Gly Ala Ile Asp Ala Asp Gly 
        35                  40                  45              


Tyr Tyr His Ala Gln Cys Gln Asp Leu Ile Thr Asp Tyr Val Leu Trp 
    50                  55                  60                  


Leu Thr Asp Asn Lys Val Arg Thr Trp Gly Asn Ala Lys Asp Gln Ile 
65                  70                  75                  80  


Lys Gln Ser Tyr Gly Thr Gly Phe Lys Ile His Glu Asn Lys Pro Ser 
                85                  90                  95      


Thr Val Pro Lys Lys Gly Trp Ile Ala Val Phe Thr Ser Gly Ser Tyr 
            100                 105                 110         


Glu Gln Trp Gly His Ile Gly Ile Val Tyr Asp Gly Gly Asn Thr Ser 
        115                 120                 125             


Thr Phe Thr Ile Leu Glu Gln Asn Trp Asn Gly Tyr Ala Asn Lys Lys 
    130                 135                 140                 


Pro Thr Lys Arg Val Asp Asn Tyr Tyr Gly Leu Thr His Phe Ile Glu 
145                 150                 155                 160 


Ile Pro Val Lys Ala Gly Thr Thr Val Lys Lys Glu Thr Ala Lys Lys 
                165                 170                 175     


Ser Ala Thr Ser His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys 
            180                 185                 190         


Lys Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met 
        195                 200                 205             


His Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala 
    210                 215                 220                 


Ile Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly 
225                 230                 235                 240 


Gly Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp 
                245                 250                 255     


Tyr Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys 
            260                 265                 270         


Ala Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala 
        275                 280                 285             


Pro His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr 
    290                 295                 300                 


Ala Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala 
305                 310                 315                 320 


Gly Gly Thr Val Thr Pro Thr Pro Asn Pro Gly Asn Gly Tyr Lys Thr 
                325                 330                 335     


Asn Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr Ala 
            340                 345                 350         


Asn Thr Asp Ile Ile Thr Arg Leu Thr Gly Pro Phe Arg Ser Met Pro 
        355                 360                 365             


Gln Ser Gly Val Leu Arg Lys Gly Leu Thr Ile Lys Tyr Asp Glu Val 
    370                 375                 380                 


Met Lys Gln Asp Gly His Val Trp Val Gly Tyr Asn Thr Asn Ser Gly 
385                 390                 395                 400 


Lys Arg Val Tyr Leu Pro Val Arg Thr Trp Asn Glu Ser Thr Gly Glu 
                405                 410                 415     


Leu Gly Pro Leu Trp Gly Thr Ile Lys His His His His His His Leu 
            420                 425                 430         


Lys His Pro Gln Tyr Asn Gln Arg 
        435                 440 


<210>  106
<211>  438
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Fusion protein comprising CHAP domain of LysK endolysin, M23 
       endopeptidase domain of lysostaphin, cell wall binding domain of 
       ALE-1, His6-tag and synthetic peptide KNK; w/o N-terminal 
       methionine

<400>  106

Ala Lys Thr Gln Ala Glu Ile Asn Lys Arg Leu Asp Ala Tyr Ala Lys 
1               5                   10                  15      


Gly Thr Val Asp Ser Pro Tyr Arg Val Lys Lys Ala Thr Ser Tyr Asp 
            20                  25                  30          


Pro Ser Phe Gly Val Met Glu Ala Gly Ala Ile Asp Ala Asp Gly Tyr 
        35                  40                  45              


Tyr His Ala Gln Cys Gln Asp Leu Ile Thr Asp Tyr Val Leu Trp Leu 
    50                  55                  60                  


Thr Asp Asn Lys Val Arg Thr Trp Gly Asn Ala Lys Asp Gln Ile Lys 
65                  70                  75                  80  


Gln Ser Tyr Gly Thr Gly Phe Lys Ile His Glu Asn Lys Pro Ser Thr 
                85                  90                  95      


Val Pro Lys Lys Gly Trp Ile Ala Val Phe Thr Ser Gly Ser Tyr Glu 
            100                 105                 110         


Gln Trp Gly His Ile Gly Ile Val Tyr Asp Gly Gly Asn Thr Ser Thr 
        115                 120                 125             


Phe Thr Ile Leu Glu Gln Asn Trp Asn Gly Tyr Ala Asn Lys Lys Pro 
    130                 135                 140                 


Thr Lys Arg Val Asp Asn Tyr Tyr Gly Leu Thr His Phe Ile Glu Ile 
145                 150                 155                 160 


Pro Val Lys Ala Gly Thr Thr Val Lys Lys Glu Thr Ala Lys Lys Ser 
                165                 170                 175     


Ala Thr Ser His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys Lys 
            180                 185                 190         


Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His 
        195                 200                 205             


Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile 
    210                 215                 220                 


Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly 
225                 230                 235                 240 


Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp Tyr 
                245                 250                 255     


Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala 
            260                 265                 270         


Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro 
        275                 280                 285             


His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala 
    290                 295                 300                 


Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly 
305                 310                 315                 320 


Gly Thr Val Thr Pro Thr Pro Asn Pro Gly Asn Gly Tyr Lys Thr Asn 
                325                 330                 335     


Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr Ala Asn 
            340                 345                 350         


Thr Asp Ile Ile Thr Arg Leu Thr Gly Pro Phe Arg Ser Met Pro Gln 
        355                 360                 365             


Ser Gly Val Leu Arg Lys Gly Leu Thr Ile Lys Tyr Asp Glu Val Met 
    370                 375                 380                 


Lys Gln Asp Gly His Val Trp Val Gly Tyr Asn Thr Asn Ser Gly Lys 
385                 390                 395                 400 


Arg Val Tyr Leu Pro Val Arg Thr Trp Asn Glu Ser Thr Gly Glu Leu 
                405                 410                 415     


Gly Pro Leu Trp Gly Thr Ile Lys Gly Ser His His His His His His 
            420                 425                 430         


Gly Gly Ser Lys Asn Lys 
        435             


<210>  107
<211>  439
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Fusion protein comprising CHAP domain of LysK endolysin, M23 
       endopeptidase domain of lysostaphin, cell wall binding domain of 
       ALE-1, His6-tag and synthetic peptide KNK

<400>  107

Met Ala Lys Thr Gln Ala Glu Ile Asn Lys Arg Leu Asp Ala Tyr Ala 
1               5                   10                  15      


Lys Gly Thr Val Asp Ser Pro Tyr Arg Val Lys Lys Ala Thr Ser Tyr 
            20                  25                  30          


Asp Pro Ser Phe Gly Val Met Glu Ala Gly Ala Ile Asp Ala Asp Gly 
        35                  40                  45              


Tyr Tyr His Ala Gln Cys Gln Asp Leu Ile Thr Asp Tyr Val Leu Trp 
    50                  55                  60                  


Leu Thr Asp Asn Lys Val Arg Thr Trp Gly Asn Ala Lys Asp Gln Ile 
65                  70                  75                  80  


Lys Gln Ser Tyr Gly Thr Gly Phe Lys Ile His Glu Asn Lys Pro Ser 
                85                  90                  95      


Thr Val Pro Lys Lys Gly Trp Ile Ala Val Phe Thr Ser Gly Ser Tyr 
            100                 105                 110         


Glu Gln Trp Gly His Ile Gly Ile Val Tyr Asp Gly Gly Asn Thr Ser 
        115                 120                 125             


Thr Phe Thr Ile Leu Glu Gln Asn Trp Asn Gly Tyr Ala Asn Lys Lys 
    130                 135                 140                 


Pro Thr Lys Arg Val Asp Asn Tyr Tyr Gly Leu Thr His Phe Ile Glu 
145                 150                 155                 160 


Ile Pro Val Lys Ala Gly Thr Thr Val Lys Lys Glu Thr Ala Lys Lys 
                165                 170                 175     


Ser Ala Thr Ser His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys 
            180                 185                 190         


Lys Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met 
        195                 200                 205             


His Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala 
    210                 215                 220                 


Ile Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly 
225                 230                 235                 240 


Gly Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp 
                245                 250                 255     


Tyr Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys 
            260                 265                 270         


Ala Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala 
        275                 280                 285             


Pro His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr 
    290                 295                 300                 


Ala Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala 
305                 310                 315                 320 


Gly Gly Thr Val Thr Pro Thr Pro Asn Pro Gly Asn Gly Tyr Lys Thr 
                325                 330                 335     


Asn Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr Ala 
            340                 345                 350         


Asn Thr Asp Ile Ile Thr Arg Leu Thr Gly Pro Phe Arg Ser Met Pro 
        355                 360                 365             


Gln Ser Gly Val Leu Arg Lys Gly Leu Thr Ile Lys Tyr Asp Glu Val 
    370                 375                 380                 


Met Lys Gln Asp Gly His Val Trp Val Gly Tyr Asn Thr Asn Ser Gly 
385                 390                 395                 400 


Lys Arg Val Tyr Leu Pro Val Arg Thr Trp Asn Glu Ser Thr Gly Glu 
                405                 410                 415     


Leu Gly Pro Leu Trp Gly Thr Ile Lys Gly Ser His His His His His 
            420                 425                 430         


His Gly Gly Ser Lys Asn Lys 
        435                 


<210>  108
<211>  438
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Fusion protein comprising CHAP domain of LysK endolysin, M23 
       endopeptidase domain of lysostaphin, cell wall binding domain of 
       ALE-1, His6-tag and synthetic peptide KNK; incl. mutations K17E 
       and H51Q; w/o N-terminal methionine

<400>  108

Ala Lys Thr Gln Ala Glu Ile Asn Lys Arg Leu Asp Ala Tyr Ala Glu 
1               5                   10                  15      


Gly Thr Val Asp Ser Pro Tyr Arg Val Lys Lys Ala Thr Ser Tyr Asp 
            20                  25                  30          


Pro Ser Phe Gly Val Met Glu Ala Gly Ala Ile Asp Ala Asp Gly Tyr 
        35                  40                  45              


Tyr Gln Ala Gln Cys Gln Asp Leu Ile Thr Asp Tyr Val Leu Trp Leu 
    50                  55                  60                  


Thr Asp Asn Lys Val Arg Thr Trp Gly Asn Ala Lys Asp Gln Ile Lys 
65                  70                  75                  80  


Gln Ser Tyr Gly Thr Gly Phe Lys Ile His Glu Asn Lys Pro Ser Thr 
                85                  90                  95      


Val Pro Lys Lys Gly Trp Ile Ala Val Phe Thr Ser Gly Ser Tyr Glu 
            100                 105                 110         


Gln Trp Gly His Ile Gly Ile Val Tyr Asp Gly Gly Asn Thr Ser Thr 
        115                 120                 125             


Phe Thr Ile Leu Glu Gln Asn Trp Asn Gly Tyr Ala Asn Lys Lys Pro 
    130                 135                 140                 


Thr Lys Arg Val Asp Asn Tyr Tyr Gly Leu Thr His Phe Ile Glu Ile 
145                 150                 155                 160 


Pro Val Lys Ala Gly Thr Thr Val Lys Lys Glu Thr Ala Lys Lys Ser 
                165                 170                 175     


Ala Thr Ser His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys Lys 
            180                 185                 190         


Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His 
        195                 200                 205             


Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile 
    210                 215                 220                 


Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly 
225                 230                 235                 240 


Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp Tyr 
                245                 250                 255     


Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala 
            260                 265                 270         


Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro 
        275                 280                 285             


His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala 
    290                 295                 300                 


Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly 
305                 310                 315                 320 


Gly Thr Val Thr Pro Thr Pro Asn Pro Gly Asn Gly Tyr Lys Thr Asn 
                325                 330                 335     


Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr Ala Asn 
            340                 345                 350         


Thr Asp Ile Ile Thr Arg Leu Thr Gly Pro Phe Arg Ser Met Pro Gln 
        355                 360                 365             


Ser Gly Val Leu Arg Lys Gly Leu Thr Ile Lys Tyr Asp Glu Val Met 
    370                 375                 380                 


Lys Gln Asp Gly His Val Trp Val Gly Tyr Asn Thr Asn Ser Gly Lys 
385                 390                 395                 400 


Arg Val Tyr Leu Pro Val Arg Thr Trp Asn Glu Ser Thr Gly Glu Leu 
                405                 410                 415     


Gly Pro Leu Trp Gly Thr Ile Lys Gly Ser His His His His His His 
            420                 425                 430         


Gly Gly Ser Lys Asn Lys 
        435             


<210>  109
<211>  439
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein comprising CHAP domain of LysK endolysin, M23 
       endopeptidase domain of lysostaphin, cell wall binding domain of 
       ALE-1, His6-tag and synthetic peptide KNK; incl. mutations K17E 
       and H51Q

<400>  109

Met Ala Lys Thr Gln Ala Glu Ile Asn Lys Arg Leu Asp Ala Tyr Ala 
1               5                   10                  15      


Glu Gly Thr Val Asp Ser Pro Tyr Arg Val Lys Lys Ala Thr Ser Tyr 
            20                  25                  30          


Asp Pro Ser Phe Gly Val Met Glu Ala Gly Ala Ile Asp Ala Asp Gly 
        35                  40                  45              


Tyr Tyr Gln Ala Gln Cys Gln Asp Leu Ile Thr Asp Tyr Val Leu Trp 
    50                  55                  60                  


Leu Thr Asp Asn Lys Val Arg Thr Trp Gly Asn Ala Lys Asp Gln Ile 
65                  70                  75                  80  


Lys Gln Ser Tyr Gly Thr Gly Phe Lys Ile His Glu Asn Lys Pro Ser 
                85                  90                  95      


Thr Val Pro Lys Lys Gly Trp Ile Ala Val Phe Thr Ser Gly Ser Tyr 
            100                 105                 110         


Glu Gln Trp Gly His Ile Gly Ile Val Tyr Asp Gly Gly Asn Thr Ser 
        115                 120                 125             


Thr Phe Thr Ile Leu Glu Gln Asn Trp Asn Gly Tyr Ala Asn Lys Lys 
    130                 135                 140                 


Pro Thr Lys Arg Val Asp Asn Tyr Tyr Gly Leu Thr His Phe Ile Glu 
145                 150                 155                 160 


Ile Pro Val Lys Ala Gly Thr Thr Val Lys Lys Glu Thr Ala Lys Lys 
                165                 170                 175     


Ser Ala Thr Ser His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys 
            180                 185                 190         


Lys Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met 
        195                 200                 205             


His Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala 
    210                 215                 220                 


Ile Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly 
225                 230                 235                 240 


Gly Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp 
                245                 250                 255     


Tyr Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys 
            260                 265                 270         


Ala Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala 
        275                 280                 285             


Pro His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr 
    290                 295                 300                 


Ala Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala 
305                 310                 315                 320 


Gly Gly Thr Val Thr Pro Thr Pro Asn Pro Gly Asn Gly Tyr Lys Thr 
                325                 330                 335     


Asn Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr Ala 
            340                 345                 350         


Asn Thr Asp Ile Ile Thr Arg Leu Thr Gly Pro Phe Arg Ser Met Pro 
        355                 360                 365             


Gln Ser Gly Val Leu Arg Lys Gly Leu Thr Ile Lys Tyr Asp Glu Val 
    370                 375                 380                 


Met Lys Gln Asp Gly His Val Trp Val Gly Tyr Asn Thr Asn Ser Gly 
385                 390                 395                 400 


Lys Arg Val Tyr Leu Pro Val Arg Thr Trp Asn Glu Ser Thr Gly Glu 
                405                 410                 415     


Leu Gly Pro Leu Trp Gly Thr Ile Lys Gly Ser His His His His His 
            420                 425                 430         


His Gly Gly Ser Lys Asn Lys 
        435                 


<210>  110
<211>  438
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Fusion protein comprising CHAP domain of LysK endolysin, M23 
       endopeptidase domain of lysostaphin, cell wall binding domain of 
       ALE-1, His6-tag and synthetic peptide KNK; incl. mutations 
       K17E,H51Q, K28N, T86A and G154S; w/o N-terminal methionine

<400>  110

Ala Lys Thr Gln Ala Glu Ile Asn Lys Arg Leu Asp Ala Tyr Ala Glu 
1               5                   10                  15      


Gly Thr Val Asp Ser Pro Tyr Arg Val Lys Asn Ala Thr Ser Tyr Asp 
            20                  25                  30          


Pro Ser Phe Gly Val Met Glu Ala Gly Ala Ile Asp Ala Asp Gly Tyr 
        35                  40                  45              


Tyr Gln Ala Gln Cys Gln Asp Leu Ile Thr Asp Tyr Val Leu Trp Leu 
    50                  55                  60                  


Thr Asp Asn Lys Val Arg Thr Trp Gly Asn Ala Lys Asp Gln Ile Lys 
65                  70                  75                  80  


Gln Ser Tyr Gly Ala Gly Phe Lys Ile His Glu Asn Lys Pro Ser Thr 
                85                  90                  95      


Val Pro Lys Lys Gly Trp Ile Ala Val Phe Thr Ser Gly Ser Tyr Glu 
            100                 105                 110         


Gln Trp Gly His Ile Gly Ile Val Tyr Asp Gly Gly Asn Thr Ser Thr 
        115                 120                 125             


Phe Thr Ile Leu Glu Gln Asn Trp Asn Gly Tyr Ala Asn Lys Lys Pro 
    130                 135                 140                 


Thr Lys Arg Val Asp Asn Tyr Tyr Ser Leu Thr His Phe Ile Glu Ile 
145                 150                 155                 160 


Pro Val Lys Ala Gly Thr Thr Val Lys Lys Glu Thr Ala Lys Lys Ser 
                165                 170                 175     


Ala Thr Ser His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys Lys 
            180                 185                 190         


Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His 
        195                 200                 205             


Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile 
    210                 215                 220                 


Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly 
225                 230                 235                 240 


Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp Tyr 
                245                 250                 255     


Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala 
            260                 265                 270         


Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro 
        275                 280                 285             


His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala 
    290                 295                 300                 


Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly 
305                 310                 315                 320 


Gly Thr Val Thr Pro Thr Pro Asn Pro Gly Asn Gly Tyr Lys Thr Asn 
                325                 330                 335     


Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr Ala Asn 
            340                 345                 350         


Thr Asp Ile Ile Thr Arg Leu Thr Gly Pro Phe Arg Ser Met Pro Gln 
        355                 360                 365             


Ser Gly Val Leu Arg Lys Gly Leu Thr Ile Lys Tyr Asp Glu Val Met 
    370                 375                 380                 


Lys Gln Asp Gly His Val Trp Val Gly Tyr Asn Thr Asn Ser Gly Lys 
385                 390                 395                 400 


Arg Val Tyr Leu Pro Val Arg Thr Trp Asn Glu Ser Thr Gly Glu Leu 
                405                 410                 415     


Gly Pro Leu Trp Gly Thr Ile Lys Gly Ser His His His His His His 
            420                 425                 430         


Gly Gly Ser Lys Asn Lys 
        435             


<210>  111
<211>  439
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Fusion protein comprising CHAP domain of LysK endolysin, M23 
       endopeptidase domain of lysostaphin, cell wall binding domain of 
       ALE-1, His6-tag and synthetic peptide KNK; incl. mutations 
       K17E,H51Q, K28N, T86A and G154S

<400>  111

Met Ala Lys Thr Gln Ala Glu Ile Asn Lys Arg Leu Asp Ala Tyr Ala 
1               5                   10                  15      


Glu Gly Thr Val Asp Ser Pro Tyr Arg Val Lys Asn Ala Thr Ser Tyr 
            20                  25                  30          


Asp Pro Ser Phe Gly Val Met Glu Ala Gly Ala Ile Asp Ala Asp Gly 
        35                  40                  45              


Tyr Tyr Gln Ala Gln Cys Gln Asp Leu Ile Thr Asp Tyr Val Leu Trp 
    50                  55                  60                  


Leu Thr Asp Asn Lys Val Arg Thr Trp Gly Asn Ala Lys Asp Gln Ile 
65                  70                  75                  80  


Lys Gln Ser Tyr Gly Ala Gly Phe Lys Ile His Glu Asn Lys Pro Ser 
                85                  90                  95      


Thr Val Pro Lys Lys Gly Trp Ile Ala Val Phe Thr Ser Gly Ser Tyr 
            100                 105                 110         


Glu Gln Trp Gly His Ile Gly Ile Val Tyr Asp Gly Gly Asn Thr Ser 
        115                 120                 125             


Thr Phe Thr Ile Leu Glu Gln Asn Trp Asn Gly Tyr Ala Asn Lys Lys 
    130                 135                 140                 


Pro Thr Lys Arg Val Asp Asn Tyr Tyr Ser Leu Thr His Phe Ile Glu 
145                 150                 155                 160 


Ile Pro Val Lys Ala Gly Thr Thr Val Lys Lys Glu Thr Ala Lys Lys 
                165                 170                 175     


Ser Ala Thr Ser His Glu His Ser Ala Gln Trp Leu Asn Asn Tyr Lys 
            180                 185                 190         


Lys Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met 
        195                 200                 205             


His Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala 
    210                 215                 220                 


Ile Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly 
225                 230                 235                 240 


Gly Asn Gln Ile Gly Leu Ile Glu Asn Asp Gly Val His Arg Gln Trp 
                245                 250                 255     


Tyr Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys 
            260                 265                 270         


Ala Gly Gln Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala 
        275                 280                 285             


Pro His Leu His Phe Gln Arg Met Val Asn Ser Phe Ser Asn Ser Thr 
    290                 295                 300                 


Ala Gln Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala 
305                 310                 315                 320 


Gly Gly Thr Val Thr Pro Thr Pro Asn Pro Gly Asn Gly Tyr Lys Thr 
                325                 330                 335     


Asn Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr Ala 
            340                 345                 350         


Asn Thr Asp Ile Ile Thr Arg Leu Thr Gly Pro Phe Arg Ser Met Pro 
        355                 360                 365             


Gln Ser Gly Val Leu Arg Lys Gly Leu Thr Ile Lys Tyr Asp Glu Val 
    370                 375                 380                 


Met Lys Gln Asp Gly His Val Trp Val Gly Tyr Asn Thr Asn Ser Gly 
385                 390                 395                 400 


Lys Arg Val Tyr Leu Pro Val Arg Thr Trp Asn Glu Ser Thr Gly Glu 
                405                 410                 415     


Leu Gly Pro Leu Trp Gly Thr Ile Lys Gly Ser His His His His His 
            420                 425                 430         


His Gly Gly Ser Lys Asn Lys 
        435                 


